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Articles 91 - 103 of 103
Full-Text Articles in Life Sciences
Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin
Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin
Turkish Journal of Biology
Wheat landraces could be unique sources of favorable genes for agronomic traits like yield and insect, pest, and disease resistances, as well as quality characteristics that are important for breeding programs. Molecular marker systems offer great opportunities to characterize wheat accessions, which in particular are not morphologically identifiable. In this study, 20 bread wheat landraces collected from different regions of Turkey were characterized by using microsatellite markers (SSRs) and morphological characters. Seventeen morphological characters were used. Fifteen SSR primers were prescreened and the 7 most polymorphic primers were employed in characterization. The most polymorphic SSR loci were Xgwm 95 and …
Utilization Of Gold Nanostructures In Biomedical Applications, Gamze Tan, Mehmet Ali̇ Onur, Necdet Sağlam
Utilization Of Gold Nanostructures In Biomedical Applications, Gamze Tan, Mehmet Ali̇ Onur, Necdet Sağlam
Turkish Journal of Biology
Every living organism on earth owes its viability to its different sizes of nanostructures and the interaction of these structures at the nano size. Nanotechnology gives us an opportunity to understand nanoscale processes in living organisms and interfere with and manipulate them. Today, biocompatible nanosized structures are designed by applying developments in nanotechnology to biomedicine; thus, therapeutic agents are available to reach diseased tissues and even cells. However, it is essential that nanomaterials that would be used for therapeutic aims be targetable to diseased areas and have low toxicity. In addition, these nanomaterials must have high biocirculation and pharmacokinetic properties. …
Effect Of Exogenous Application Of Ascorbic Acid On Antioxidant Enzyme Activities, Proline Contents, And Growth Parameters Of Saccharum Spp. Hybrid Cv. Hsf-240 Under Salt Stress, Batool Ejaz, Zahoor Ahmad Sajid, Faheem Aftab
Effect Of Exogenous Application Of Ascorbic Acid On Antioxidant Enzyme Activities, Proline Contents, And Growth Parameters Of Saccharum Spp. Hybrid Cv. Hsf-240 Under Salt Stress, Batool Ejaz, Zahoor Ahmad Sajid, Faheem Aftab
Turkish Journal of Biology
The present study was undertaken to examine the effects of exogenous application of ascorbic acid (AA) through different modes on growth and associated biochemical parameters in Saccharum spp. hybrid cv. HSF-240, under salt stress. In a pot experiment, AA was applied through irrigation or foliar-spray at the concentrations of 0.1, 0.5, and 1 mM with or without 100 mM NaCl concentration. Vegetative growth measurements, antioxidant enzyme activities (POD and SOD), and protein and proline contents of plants were recorded to study the effects of these treatments. The presence of salt reduced the growth of sugarcane plants. The AA application not …
Dipeptidyl Peptidase Iv Production By Solid State Fermentation Using Alternative Fungal Sources, Gaye Öngen, Sayit Sargin, Özlem Üstün, Ceren Kutlu, Mesut Yücel
Dipeptidyl Peptidase Iv Production By Solid State Fermentation Using Alternative Fungal Sources, Gaye Öngen, Sayit Sargin, Özlem Üstün, Ceren Kutlu, Mesut Yücel
Turkish Journal of Biology
The present work was carried out for the production of dipeptidyl peptidase IV (DPP IV; EC 3.4.14.5) using Aspergillus, Penicillium, and Rhizopus strains under solid state fermentation conditions. Response surface methodology was applied for the optimization of the selected operational variables (corn flour, initial moisture content, and cultivation time) for DPP IV activity as the response. The optimal parameters of DPP IV activity for the independent variables, namely the amount of corn flour (% w/w), initial moisture content (% w/w), and cultivation time (days), were evaluated to be 2.44%, 60.85%, and 4.69 days, respectively, using Aspergillus awamori T116. The response …
Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur
Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur
Turkish Journal of Biology
In this study, our aim was to develop a new simple technique for isolation of mesenchymal stem cells from adipose tissue. For this purpose, mesenchymal stem cells were isolated from rat adipose tissue by using the primary explant culture technique. When the cells became confluent, they were passaged 4 times by using the standard trypsinization method with trypsin/EDTA solution. Cells at second passage were characterized by using immunofluorescence staining against CD13 and CD29 markers. The results showed that these cultured cells were positive for CD13 and CD29 markers. Flow cytometry analysis was also done against CD29, CD90, CD54, MHC Class …
Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem
Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem
Turkish Journal of Biology
An extracellular endoglucanase (EG) from Aspergillus niger VTCC-F021 was purified 2.09-fold to homogeneity with a yield of 18.4%. The enzyme had a molecular mass of 31 kDa and a specific activity of 14.122 U/mg protein. Optimum temperature was observed at 55 °C and optimum pH at 5. The enzyme was stable up to 50 °C and from pH 5 to 6 with residual activity >80% and 60%, respectively. The kinetic constants K_m and V_{max} determined for EG, with carboxyl methyl cellulose as a substrate, were 8.5815 mg CMC/mL and 20.121 U/mg protein, respectively. EDTA increased EG activity by 35% at …
Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun
Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun
Turkish Journal of Biology
Different parts and products of Juniperus species have been used as folk medicine for various infectious diseases, rheumatism, and wounds in Turkey. In order to evaluate this traditional usage, antiinflammatory and antinociceptive activities of leaf and berry extracts of 3 Juniperus species (J. excelsa, J. foetidissima, and J. sabina) were investigated. Additionally, antiinflammatory and antinociceptive activities of subextracts of J. oxycedrus subsp. oxycedrus (Joso) berries and leaves were evaluated. The p-benzoquinone-induced writhing test for antinociceptive activity and the carrageenan-induced hind paw edema model for antiinflammatory activity in mice were employed. Ethanol extract prepared from berries of J. foetidissima provided a …
Evaluation Of Nested Pcr Method For Diagnosis Of Meningitis Due To Neisseria Meningitidis And Haemophilus Influenzae, Zahra Hojjati Bonab, Safar Farajnia, Reza Ghotaslou, Elhameh Nikkhah
Evaluation Of Nested Pcr Method For Diagnosis Of Meningitis Due To Neisseria Meningitidis And Haemophilus Influenzae, Zahra Hojjati Bonab, Safar Farajnia, Reza Ghotaslou, Elhameh Nikkhah
Turkish Journal of Biology
Meningitis due to Neisseria meningitidis and Haemophilus influenzae is a common infectious disease among infants and children that can be fatal in the absence of rapid and proper diagnosis and treatment. Therefore, a rapid and sensitive method for diagnosis is a high priority. The aim of this study was to compare the performance of culture, conventional polymerase chain reaction (PCR), and nested PCR methods for diagnosis of meningitis caused by N. meningitidis and H. influenzae. A total of 106 cerebrospinal fluid samples were collected from infants and children suspected of having meningitis. A portion of these samples were used for …
Identification And Isolation Of Salt-Stress-Responsive Transcripts From Gossypium Arboreum L., Muhammad Naveed Shahid, Adil Jamal, Bushra Rashid, Beenish Aftab, Tayyab Husnain
Identification And Isolation Of Salt-Stress-Responsive Transcripts From Gossypium Arboreum L., Muhammad Naveed Shahid, Adil Jamal, Bushra Rashid, Beenish Aftab, Tayyab Husnain
Turkish Journal of Biology
Salinity has adverse effects on plant development and ultimately reduces production. Very few reports on the genes induced by salt stress in Gossypium arboreum are available. In this report salt-stress-responsive genes were screened from cotton leaves using the differential display (DD) technique, and gene expression was compared under control and salt stressed conditions. By using a 107 primer combination, 25 gene fragments were found to be up-regulated in response to salt stress. Out of 25 induced fragments, 12 were rejected as false positives after reamplification and quality control assay. The remaining 13 fragments were selected for cloning and genetic transformation. …
Full Issue: Volume 5, Issue 2
The Science Journal of the Lander College of Arts and Sciences
No abstract provided.
Full Issue: Volume 6, Number 1
Full Issue: Volume 6, Number 1
The Science Journal of the Lander College of Arts and Sciences
No abstract provided.
Current Theories On The Human Sex Ratio, Yisroel Cofsky
Current Theories On The Human Sex Ratio, Yisroel Cofsky
The Science Journal of the Lander College of Arts and Sciences
The human sex ratio is skewed toward males, and this is the subject of much research. A review of some of the theories currently available was conducted, searching and analyzing the current scientific literature. Suggestions to explain the ratio include unequal proportions of X and Y sperm, the effect of maternal diet, maternal testosterone levels, and the natural fluctuations in the consistency of the cervical mucus. Although the difference in X/Y sperm proportions does not adequately explain all the data, the other theories do seem to explain the greater percentage of male births. However, there is still not enough information …
Management In A Neotropical Show Cave: Planning For Invertebrates Conservation, Thais Giovannini Pellegrini, Rodrigo Lopes Ferreira
Management In A Neotropical Show Cave: Planning For Invertebrates Conservation, Thais Giovannini Pellegrini, Rodrigo Lopes Ferreira
International Journal of Speleology
Lapa Nova is a dolomitic cave about 4.5 km long located in northwestern Minas Gerais state, Brazil. The cave experiences intense tourism, concentrated over a single period of the year, during the Feast of Our Lady of Lapa. In order to evaluate the impacts felt by the invertebrate community from this tourism, a new methodology was proposed. Four types of areas (intense visitation area, outlying visitation areas, moderate visitation areas and no-visitation areas) were sampled for invertebrates. There was one sampling prior and another on the last day of the 128th feast, to evaluate the effects of visitation on …