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Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu
Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu
Turkish Journal of Biology
Salt-tolerant mutants of potato (Solanum tuberosum L. 'Marfona') were obtained via gamma irradiation. Node explants of the Marfona potato plant were treated with various dosages of gamma irradiation, and the M_1V_2 and M_1V_3 clonal generations were developed. Selection of salt-tolerant mutants was accomplished by in vitro selection media containing 50, 100, and 125 mM NaCl. Molecular-level differences between the control and mutant plants were elucidated using RAPD-PCR method, and the polymorphism rate according to the selected primers was calculated as 89.66%. Genetic distances between the controls and mutants were also calculated, and related dendrograms were produced. On average the mutants …
Determination Of Genetic Relationships Among Velezia L. Caryophyllaceae) Species Using Rapd Markers, İlham Eröz Poyraz, Emel Sözen, Ebru Ataşlar, İsmai̇l Poyraz
Determination Of Genetic Relationships Among Velezia L. Caryophyllaceae) Species Using Rapd Markers, İlham Eröz Poyraz, Emel Sözen, Ebru Ataşlar, İsmai̇l Poyraz
Turkish Journal of Biology
Random amplified polymorphic DNA (RAPD) markers were used to determine genetic relationships among Velezia L. species from Turkey. A total of 432 amplified bands were obtained using 14 RAPD primers. The polymorphism in RAPD markers was high (98.61%) and was sufficient to distinguish each species. The degree of band-sharing was used for evaluating the genetic similarity between species and for constructing a dendrogram by the unweighted pair group method with arithmetic mean (UPGMA). Genetic relationships among the species were found to be fully consistent with those obtained by the use of morphological characters. Data obtained from our study demonstrated that …