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A Preliminary Attempt For Efficient Genetic Transformation And Regeneration Of Legume Mucuna Pruriens L. Mediated By Agrobacterium Tumefaciens, Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyl.Kumaran Ramesh, Mahadevappa Paresha, Mahaboob Habeebulla Moinuddin Khan
A Preliminary Attempt For Efficient Genetic Transformation And Regeneration Of Legume Mucuna Pruriens L. Mediated By Agrobacterium Tumefaciens, Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyl.Kumaran Ramesh, Mahadevappa Paresha, Mahaboob Habeebulla Moinuddin Khan
Turkish Journal of Biology
Mucuna pruriens belongs to the family Papillonaceae. The seeds and leaves of these plants contain a large amount of L-DOPA. L-DOPA (L-3,4-dihydroxyphenylalanine) is one of the most widely used drugs in the treatment of Parkinson disease. Development of an efficient gene transfer method is an absolute requirement for genetically improving Mucuna pruriens and creating plants with more desirable traits. A simple protocol was developed for the Agrobacterium-mediated stable genetic transformation of M. pruriens. Agrobacterium tumefaciens strain EHA 101, containing the vector pCAMBIA1305 and the hptII and GUS plus genes, was used for the gene transfer experiments. Putative transgenic shoots were …
Evaluation Of Nested Pcr Method For Diagnosis Of Meningitis Due To Neisseria Meningitidis And Haemophilus Influenzae, Zahra Hojjati Bonab, Safar Farajnia, Reza Ghotaslou, Elhameh Nikkhah
Evaluation Of Nested Pcr Method For Diagnosis Of Meningitis Due To Neisseria Meningitidis And Haemophilus Influenzae, Zahra Hojjati Bonab, Safar Farajnia, Reza Ghotaslou, Elhameh Nikkhah
Turkish Journal of Biology
Meningitis due to Neisseria meningitidis and Haemophilus influenzae is a common infectious disease among infants and children that can be fatal in the absence of rapid and proper diagnosis and treatment. Therefore, a rapid and sensitive method for diagnosis is a high priority. The aim of this study was to compare the performance of culture, conventional polymerase chain reaction (PCR), and nested PCR methods for diagnosis of meningitis caused by N. meningitidis and H. influenzae. A total of 106 cerebrospinal fluid samples were collected from infants and children suspected of having meningitis. A portion of these samples were used for …