Schirmer Tear Test 1 As A Method To Measure Tear Production Value In Normal Kintamani Dogs,
2021
Chulalongkorn University
Schirmer Tear Test 1 As A Method To Measure Tear Production Value In Normal Kintamani Dogs, Steven Dwi Purbantoro, Erika Kadek Yudha Ariawan, Endah Rahmawati, Zefanya Christiani, Sri Kayati Widyastuti
The Thai Journal of Veterinary Medicine
Keratoconjunctivitis sicca (KCS) is a dry eye condition due to an inadequacy of the eyes to produce tears. This condition can be diagnosed using the Schirmer Tear Test 1 (STT1). The Kintamani dog, a Chinese Chow Chow-derivate, is now popular globally to be kept by dog lovers. This study aims to determine the normal value of tear production in Kintamani dogs. Forty eyes from above one-year-old twenty Kintamani dogs were used in this study. Tear production value was measured using STT1 strip. The result showed that the mean of tear production in Kintamani dogs was 23.37±4.415 mm/min. Statistically, there was …
Immunostimulatory Effect Of Disinfectant On Bees,
2021
Chulalongkorn University
Immunostimulatory Effect Of Disinfectant On Bees, Tatiana Romanishina, Svitlana Guralska, Tetiana Kot, Svitlana Furman, Oleh Pinsky, Diana Feshchenko, Zhanna Rybachuk, Olexiy Tkachenko, Olga Zastulka, Svitlana Tushak
The Thai Journal of Veterinary Medicine
The article presents a modern interpretation of the immunostimulatory effect of the disinfectant “Biocontact Plus” on the bee organism. The minimum threshold concentrations of the disinfectant with bactericidal effect were determined in vitro. The stimulating effect of 0.15 % of the concentration of active substances of the disinfectant has been confirmed in vivo. The immunostimulatory effect of this concentration on the immune status of bee colonies is interpreted (the number of young and mature immunocytes). The immunomodulatory effect of 0.15 % disinfectant “Biocontact Plus” on bee haemolymph allows for the use of alternative therapies for bacterial diseases of bees in …
Retrospective Survey Of The Patterns Of Prescription And Request For Vitamins, Minerals And Vitamin-Mineral Formulations For Dogs In A Veterinary Hospital In Nigeria,
2021
Chulalongkorn University
Retrospective Survey Of The Patterns Of Prescription And Request For Vitamins, Minerals And Vitamin-Mineral Formulations For Dogs In A Veterinary Hospital In Nigeria, Thelma Ebele Ihedioha, Isaac Uzoma Asuzu, John Anaelom Nwanta
The Thai Journal of Veterinary Medicine
This study evaluated the patterns of prescription/request of vitamins, minerals and vitamin-mineral formulations (V-MF) for dogs presented for veterinary care at the Veterinary Teaching Hospital Nsukka (VTHN), Nigeria, between January 2013 and December 2017. Hospital records of a total of 4074 dogs were used for the study. Overall, vitamins, minerals and V-MF were prescribed/requested for 3096 out of the 4074 cases (75.33%). The frequency of prescription/requests for vitamins, minerals and V-MF significantly (p =0.000) increased from 54.1% in 2013 to 87.8% (highest) recorded in 2016 and the effect size of the variations across time was very strong (ɸc=0.268). Vitamin B-Complex …
Reversible Pulmonary Hypertension Secondary To Bronchopneumonia In A Kitten,
2021
Chulalongkorn University
Reversible Pulmonary Hypertension Secondary To Bronchopneumonia In A Kitten, Jidapa Tosuwan, Sirilak Disatian Surachetpong
The Thai Journal of Veterinary Medicine
A five-month-old Siamese kitten was presented with acute respiratory distress. The kitten had respiratory acidosis. The result of the feline N-terminal prohormone of brain natriuretic peptide (NT-proBNP) test kit was abnormal. Thoracic radiography revealed patchy lung infiltration and cardiomegaly. Severe right heart enlargement and an intermediate probability of pulmonary hypertension were suggested from echocardiography. Pulmonary hypertension secondary to severe pneumonia was suspected. The kitten was treated with oxygen supplementation, antibiotics and pimobendan. The clinical signs, radiography and echocardiography returned to normal after 30 days of treatment.
The Efficiency Of Herbal On Blood Clotting And Wound Healing Of Ear Notched Pigs,
2021
Chulalongkorn University
The Efficiency Of Herbal On Blood Clotting And Wound Healing Of Ear Notched Pigs, Kittiphong Tippaya, Ruttayaporn Ngasaman, Kridda Chukiatsiri
The Thai Journal of Veterinary Medicine
This study aimed to determine the efficiency of Jatropha podagrica Hook. f., Chromolaena odorata (L.) and Centella asiatica (L.) on the reduction of blood clotting and promoting wound healing in notched piglets. Thirty-four notched piglets aged 2–3 days were selected for applying fresh exudes of J. podagrica Hook. f. to the right ear and povidone iodine to the left ear as a control. In the experiment of C. odorata (L.) and C. asiatica (L.), fifty notched piglets were divided into five groups and 10, 20, 30, 40 and 50% each of herbal gel was applied to the right ear and …
Zinc Supplementation Improves Semen Quality In Boars,
2021
Chulalongkorn University
Zinc Supplementation Improves Semen Quality In Boars, Supitcha Kaewma, Sanya Suphappornchai, Junpen Suwimonteerabutr, Nutthee Am-In, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
One of the causes of infertility in boars is nutritional deficiency. Interestingly, zinc has been used for improving semen quality in boars. Thus, the objective of this study was to investigate the effect of zinc as feed supplementation in order to improve semen quality in infertile boars. Three purebred Pietrain boars with a poor semen quality history were selected for the experiment. The semen samples were collected once a week for 4 weeks before the treatment period and the semen evaluated immediately. Fifteen grams (a total concentration of 150 ppm) of zinc were supplemented daily in the normal feed. The …
The Effects Of Endometrial Epithelium-Derived Exosomes On The Recruitment And Activation Of T Lymphocytes In The Uteri Of Dairy Cows,
2021
Chulalongkorn University
The Effects Of Endometrial Epithelium-Derived Exosomes On The Recruitment And Activation Of T Lymphocytes In The Uteri Of Dairy Cows, Hong An, Zhe Shi, Bofan Fu, Xinxin Yao, Hemin Ni, Yong Guo, Longfei Xiao, Xiangguo Wang
The Thai Journal of Veterinary Medicine
Endometritis is caused by endometrial cell injury and chronic inflammation of uteri infected by pathogens, leading to reproductive failure in dairy cows. Changes of the endometrial immune response regulates the development of endometritis. In a recent discovery, exosomes acted as a functional regulator secreted by a variety of cells, however, its regulatory mechanism in the local immune response of the uteri is still unclear. In this study, flow cytometry was performed to identify the increased number of T lymphocytes, namely Tc cells, in the uteri with endometritis while the Th and Treg cells decreased very significantly. Moreover, the functional marker …
The First Report Of Antimicrobial Residues In Cultured Tilapia Fillets In Three Provinces Of Lao Pdr,
2021
Chulalongkorn University
The First Report Of Antimicrobial Residues In Cultured Tilapia Fillets In Three Provinces Of Lao Pdr, Seangphed Phommachanh, Bundit Tengjaroenkul, Peerapol Sukon, Lamyai Neeratanaphan, Kanit Chukanhom
The Thai Journal of Veterinary Medicine
The objective of this study was to investigate antimicrobial residues in the fillets of Nile tilapia fish (n=180) from 3 provinces (Vientiane, Savannakhet Province and Champasak Province) in Lao PDR in the summer, rainy and winter seasons. Clean meat test (CMT) was used for screening the antimicrobial residue samples. Then, the antimicrobial residue groups were examined and identified by the european six-plate test (ESPT), and the concentration of each positive antimicrobial residue from the ESPT was further assessed by high-performance liquid chromatography (HPLC). The evaluation results of the three antimicrobial detection methods were 19.44% (35/180) for the CMT, 22.86% (8/35) …
Fluid Resuscitation With Ringer’S And Trometamol-Balanced Solutions In A Rat Septic Model,
2021
Chulalongkorn University
Fluid Resuscitation With Ringer’S And Trometamol-Balanced Solutions In A Rat Septic Model, Wen-Ting Ting, Chih-Hsien Wang, Yih-Sharng Chen, Jih-Jong Lee
The Thai Journal of Veterinary Medicine
Resuscitation solution is one of the main interventions provided for septic shock patients, although there is no general consensus regarding the optimal type of solution. Therefore, a trometamol-balanced solution (TBS) was designed as a resuscitation solution. The aim of this study is to assess the effects of TBS versus Ringer’s solution (RS) in a rat septic model. Septic shock was induced in 16 male Wistar-Kyoto rats through lipopolysaccharide (LPS) induction; these rats were assigned at a ratio of 1:1 to RS and TBS groups. Blood examinations were performed using an Abbott i-STAT analyzer with CG4+ (for pH, pressure of carbon …
Expression Profile Analysis Of Micrornas During The Oestrous Cycle Of Qira Black Sheep,
2021
Chulalongkorn University
Expression Profile Analysis Of Micrornas During The Oestrous Cycle Of Qira Black Sheep, Xi Chen, Hanying Chen, Song Jiang, Chaocheng Li, Xiancun Zeng
The Thai Journal of Veterinary Medicine
Ovarian follicular development is closely regulated by a multitude of genes and specific non-coding RNAs (ncRNAs), which are typically altered during the oestrous cycle. Identification of the microRNAs (miRNA) expression profiles in sheep ovaries during the oestrous cycle is a prerequisite to further understanding their functional status. This study presents a genome-wide analysis of miRNAs from three groups of Qira black sheep: oestrus (day 0), dioestrus (day 10) and pro-oestrus (day 14). A total of 545 conserved miRNAs and 206 novel miRNAs were identified. Furthermore, 229 differentially expressed miRNAs were screened in three comparisons and 16 miRNAs were found to …
Evaluation Of Poly (Ethylene Glycol–Propylene Glycol) Copolymer Bone Hemostatic Agent Containing Gentamicin For Enhancing Local Antibacterial And Anti-Inflammatory Effects In An Infected Calvarial Defect Rat Model,
2021
Chulalongkorn University
Evaluation Of Poly (Ethylene Glycol–Propylene Glycol) Copolymer Bone Hemostatic Agent Containing Gentamicin For Enhancing Local Antibacterial And Anti-Inflammatory Effects In An Infected Calvarial Defect Rat Model, Jeon-Mo Kim, Hun-Young Yoon
The Thai Journal of Veterinary Medicine
Bone wax is the most commonly used hemostatic agent in surgery; however, it is non-absorbable and may act as a nidus of infection. Thus, new hemostatic agents that are absorbable and have antibiotic effects are needed. In this study, we assessed the hemostatic ability, bioabsorption, and local antibiotic and anti-inflammatory effects of a newly developed poly(ethylene glycol–propylene glycol) (PEG-PPG) copolymer containing gentamicin (PEG-PPGg) in a calvarial defect rat model. A total of 100 Sprague Dawley rats were used in this study. Twenty were used to determine time to hemostasis and bioabsorption of PEG-PPGg. The remaining rats were assigned to four …
Systemic Aspergillosis Involving The Mediastinum Associated With Antifungal Therapy In A Dog,
2021
Chulalongkorn University
Systemic Aspergillosis Involving The Mediastinum Associated With Antifungal Therapy In A Dog, Nawat Sannamwong, Saikaew Sutayatram, Nardtiwa Chaivoravitsakul, Patharakrit Teewasutrakul, Sawang Kesdangsakonwut, Chollada Buranakarl
The Thai Journal of Veterinary Medicine
A four-year old, intact female Labrador Retriever dog was presented with a history and clinical signs of vomiting, anorexia and mild dyspnea. Further laboratory examination, by specific canine pancreatic lipase (cPL) test kit and abdominal ultrasound, indicated acute pancreatitis. Results from thoracic radiography and computerized tomography scan showed masses in the cranial and middle mediastinal areas. A cytology examination revealed pyogranulomatous inflammation with the presence of fungal hyphae whereas Aspergillus niger was isolated and identified. Systemic aspergillosis was diagnosed. Many antifungal drugs were introduced and the clinical outcomes were discussed with careful consideration.
Carp Edema Virus A Rising Threat To Global Carp Population,
2021
Chulalongkorn University
Carp Edema Virus A Rising Threat To Global Carp Population, Xolani Phaya, Jing-Xiang Zhou, Zhuo Yu Wang, Hao Wang, Jie Liang
The Thai Journal of Veterinary Medicine
Carp edema virus (CEV), a poxvirus from the family Poxviridae, was first detected in 1974 in Japan and has since spread to most parts of the world. CEV is the etiological agent of koi sleepy disease (KSD), which has adverse health effects on both koi and common carp fish species. Equally, KSD leads to detrimental financial distress to carp farmers and further threatens both food security and ecology. The onset and severity of CEV/KSD is influenced by water temperature and handling stress. The prominent clinical sign of KSD in both koi and common carp is lethargy, which is manifested when …
In Vitro Biomechanical Comparison Of Clamped Suture Knot Techniques As A Model Of Extra-Capsular Stabilization Of Canine Cruciate Ligament Repair,
2021
Chulalongkorn University
In Vitro Biomechanical Comparison Of Clamped Suture Knot Techniques As A Model Of Extra-Capsular Stabilization Of Canine Cruciate Ligament Repair, Jung-Jin Lee, Mu-Young Kim, Hun-Young Yoon
The Thai Journal of Veterinary Medicine
Six clamped suture knot techniques were compared to identify the effects on the biomechanic properties used as a model of extra-capsular stabilization. Six clamped suture knot techniques included square knot formed by clamping the first throw with toothed mosquito forceps (SQ-TM), non-toothed mosquito forceps (SQ-NTM), or needle holder (SQ-NH) and surgeon's knot formed by clamping the first throw with toothed mosquito forceps (SG-TM), non-toothed mosquito forceps (SG-NTM), or needle holder (SG-NH). Monotonic loading test was performed on each suture-loop. Initial loop tension, ultimate load, load at 3 mm elongation, elongation at failure, stiffness, and failure mode were compared among techniques. …
Molecular Identification Of Paramphistomum Epiclitum (Trematoda: Paramphistomidae) Infecting Buffaloes In An Endemic Area Of Pakistan,
2021
Chulalongkorn University
Molecular Identification Of Paramphistomum Epiclitum (Trematoda: Paramphistomidae) Infecting Buffaloes In An Endemic Area Of Pakistan, Maria Komal, Kiran Afshan, Sabika Firasat, Màrius V. Fuentes
The Thai Journal of Veterinary Medicine
This study determined the molecular characterization of Paramphistomum epiclitum using the second internal transcribed spacer (ITS-2) grouping and secondary structure analysis from buffaloes in Khyber Pakhtunkhwa province, Pakistan. Paramphistomes were collected from the rumen of 25 infected buffaloes and their DNA was separated. DNA was intensified, followed by sequencing. Phylogenetic examinations were carried out using the Maximum Composite Likelihood approach. The results revealed extensive intraspecific and interspecific varieties among different paramphistomid species. The sequences in the present study (GenBank accession number: MW481321 and MW481322) were compared to 46 reference sequences of Paramphistomum and other trematode data from GenBank. The results …
Cryopreservation Methods Of Thai Native Chicken (Pradu-Hangdum) Semen: Effect Of Different Freezing Containers On Motion And Kinetic Parameters Of Frozen-Thawed Sperm,
2021
Chulalongkorn University
Cryopreservation Methods Of Thai Native Chicken (Pradu-Hangdum) Semen: Effect Of Different Freezing Containers On Motion And Kinetic Parameters Of Frozen-Thawed Sperm, Chakorn Khunkaew, Prapas Patchanee, Sasithorn Panasophonkul, Zubing Cao, Yunhai Zhang, Anucha Sathanawongs
The Thai Journal of Veterinary Medicine
Artificial Insemination (AI) programs using qualified semen are designed to revolutionize the poultry farming sector by targeting genetic improvement along with appropriate health strategies. Hence, a prolonged preservation method of quality sperm conserves is important for successful AI outcomes. The objective of this study was to compare the effects of different type of containers; straw, cryovial tubes and pellet on the quality of the frozen-thawed Thai native chicken (Pradu-hangdum) semen. High-quality pooled semen was collected and divided into 4 groups with 12 replicates: semen preserved with BHSV extender in 1) 0.25 mL straw, 2) 0.5 mL straw, 3) 2 mL …
Major Bacterial Pathogens Of Bovine Respiratory Disease And Lung Lesions In Calves From Selected Areas Of Ethiopia,
2021
Chulalongkorn University
Major Bacterial Pathogens Of Bovine Respiratory Disease And Lung Lesions In Calves From Selected Areas Of Ethiopia, Mirtneh Akalu, Behadra Murthy, Takele Abayeneh, Esayas Gelaye
The Thai Journal of Veterinary Medicine
Bovine respiratory disease (BRD) is a potential threat and an economically detrimental disease of calf-rearing in Ethiopia. This study was designed to isolate the major bacterial pathogens associated with respiratory disease of calves and the lung lesions involved. A crosssectional study with a purposive sampling method was employed in 170 calves during the study period from April 2018 to October 2018. The Bacteriological and molecular assay revealed overall isolation of 156 (91.76%) bacterial pathogens. Gross inspection of pneumonic lungs revealed one or more lesions of hydatidosis (46.67%), emphysema (44.44%), congestion (35.56%), hemorrhage (20.0%) and atelectasis (15.56%). Bacterial pathogen identification showed …
Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus,
2021
Chulalongkorn University
Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus, Paisan Tienthai, Padet Tummaruk
The Thai Journal of Veterinary Medicine
In female pigs, the anti-Müllerian hormone (AMH) produced by granulosa cells of developing follicles plays an important role in ovarian reserves of follicles and serum AMH levels are the biological marker of gilt reproductive performance. In the present study, we determined the AMH expression during follicular growth in replacement gilts culled due to anestrus by immunohistochemical technique. A total of 20 reproductive organs from crossbred Landrace Yorkshire gilts were collected from swine commercial farms in Thailand and categorized by the reasons for culling, i.e., the anestrus group (n=12) and other causes not related to reproduction as the control group …
Dynamics And Evolution Of Genotype 1 Porcine Reproductive And Respiratory Syndrome Virus Following Its Introduction Into A Herd Concurrently Infected With Genotypes 1 And 2,
2021
Chulalongkorn University
Dynamics And Evolution Of Genotype 1 Porcine Reproductive And Respiratory Syndrome Virus Following Its Introduction Into A Herd Concurrently Infected With Genotypes 1 And 2, Thitima Tripipat, Kepalee Saeng-Chuto, Adthakorn Madapong, Christopher James Stott, Angkana Tantituvanont, Pavita Kaewprommal, Jittima Piriyapongsa, Dachrit Nilubol
The Thai Journal of Veterinary Medicine
This study was conducted to investigate the dynamics and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) genotype 1 following its introduction into a herd concurrently infected with both PRRSV genotypes. The results demonstrated that 2 novel genotype 1 groups emerged, termed clusters II and III. The emergence of cluster II suggested that this cluster of genotype 1 virus potentially correlated with the outbreak. Then, cluster III was detected with the disappearance of cluster II. The evolution of the genotype 1 virus from cluster II to cluster III is likely to be associated with immune evasion induced by amino …
The Feasibility And Image Quality Of Using Soft Embalming Cadaver Dogs And Cats For Radiographic And Ultrasonographic Training,
2021
Chulalongkorn University
The Feasibility And Image Quality Of Using Soft Embalming Cadaver Dogs And Cats For Radiographic And Ultrasonographic Training, Chutimon Thanaboonnipat, Nan Choisunirachon, Chalika Wangdee, Srireepong Kiertkrittikhoon, Voraphan Na Songkhla, Sirakarnt Dhitavat, Chanyaratt Chaichareonchon, Lampetch Vimuktalop, Kiatpichet Komin
The Thai Journal of Veterinary Medicine
Diagnostic imaging techniques, especially radiography and ultrasonography, have been applied as routine diagnostic tools in clinical practice of both human and veterinary medicine. Soft embalmed cadavers by Thiel’s method is a new method for preserving dead bodies that has been increasingly used in medical education especially in diagnostic imaging fields. The aims of this study were, first, to examine the feasibility of using canine and feline soft cadavers in radiographic and ultrasonographic study and training. Second, to evaluate the procedure score and image quality score of radiography and ultrasonography using the soft cadaver dogs and cats as the model. All …
