Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex,
2021
Chulalongkorn University
Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex, S. Kanwichai, S. Panasophonkul, J. K. Bernard, W. Suriyasathaporn
The Thai Journal of Veterinary Medicine
The objectives of this study were to determine the differences in the expression of heat shock protein, and steroidogenic and apoptosis genes by cumulus-oocyte complex (COC) of beef cattle in a tropical environment as affected by seasonal and day effect of temperature and relative humidity. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to measure gene expression in COC in different seasons including cool (Jan to Feb), summer (Mar to Apr) and rainy (May to Jul) seasons. Ambient temperature and relative humidity recorded on the day before oocyte collection were used to classify environment groups as: low temperature and low …
Boar Sperm Production In A Tropical Environment,
2021
Chulalongkorn University
Boar Sperm Production In A Tropical Environment, Janyaporn Rungruangsak, Somchai Sangkaphet, Kakanang Buranaamnuay, Panupan Pongpeng, Padet Tummaruk
The Thai Journal of Veterinary Medicine
In a tropical environment, sow productivity is strongly associated with boar sperm production. Regular monitoring of semen quality in boars that produce semen for artificial insemination is important. The objective of the present study was to investigate the boar sperm production in an evaporative cooling system in a tropical environment in association with breed and season. A total of 2878 ejaculates from 68 boars were collected from a boar stud in the western region of Thailand. The breeds consisted of 22 Duroc, 22 Yorkshire, 18 Landrace and six hybrid boars. Seasons were classified as cool, hot or rainy. Semen volume, …
Nutrient Digestibility, Fermentation Pattern, Blood Biochemical Level And Inflammatory Response Of Nulliparous Dairy Goats Fed With Various Levels Of Oil Palm Fronds,
2021
Chulalongkorn University
Nutrient Digestibility, Fermentation Pattern, Blood Biochemical Level And Inflammatory Response Of Nulliparous Dairy Goats Fed With Various Levels Of Oil Palm Fronds, Ulia Renfelia Baysi, Pramote Paengkoum, Wanwisa Ngampongsai, Chaiyawan Wattanachant, Pin Chanjula, Pitunart Noosen
The Thai Journal of Veterinary Medicine
This study was conducted to investigate the effect of oil palm frond (OPF) inclusion in total mixed ration (TMR) diets on nutrient digestibility, fermentation pattern, blood biochemical level and inflammatory response in nulliparous dairy goats. Three nulliparous female dairy goats (Saanen x Thai native, 33.88±1.78 kg) were randomly assigned in a 3x3 Latin square design. The diets consisted of 0% OPF (CON), 20% OPF (LOPF) and 40% OPF (HOPF) on a dry matter (DM) basis. The inclusion of OPF in the diets significantly increased the DM and nutrient intake of the dairy goats (p<0.05). There was no significant difference in the digestibility of DM, organic matter and crude protein of LOPF and HOPF, (p>0.05) but those were significantly higher …
Generalized Enamel Hypoplasia In A Dog: A Case Report,
2021
Chulalongkorn University
Generalized Enamel Hypoplasia In A Dog: A Case Report, Issarin Sittijarusub, Panithi Sukho, Patchamon Supannawapat, Namphung Suemanotham
The Thai Journal of Veterinary Medicine
A one-year-old, spayed female Welsh Corgi dog referred to the dental and oral clinic with chief complaint of apparent pitted, rough teeth surfaces, stained a tan to dark brown color. The dog was diagnosed with diffused enamel hypoplasia with no evidence of endodontic disease or malformation of tooth root. A restorative procedure with nanohybrid composite resin was performed. After treatment, the dog showed a good outcome with a smooth tooth surface, restorative material replaced to cover the previous defect and no evidence of pain or discomfort. Even though enamel hypoplasia is a rare, this condition remains an important problem that …
Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province,
2021
Chulalongkorn University
Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province, Patchanee Sringam, Peerapol Sukon, Sarawut Sringam, Sunpetch Angkititrakul
The Thai Journal of Veterinary Medicine
Considering the impact of Salmonella in turtles and the role of turtles as a source of Salmonella outbreaks in humans, knowledge of Salmonella status in turtles is important. A cross-sectional study was conducted to investigate the prevalence, risk factors and antimicrobial resistance of Salmonella infections in turtles. Cloacal swabs were collected from 378 turtles in Turtle Villages located in Khon Kaen province of northeastern Thailand between January 2018 and April 2019. All samples were examined for Salmonella spp. isolation and identification by ISO 6597:2002. The overall prevalence of Salmonella in the turtles was 37.57% (142/378). Habitat, season and sex were …
Expression Of Recombinant S2 Domain Of Spike Protein Of Porcine Epidemic Diarrhea Virus,
2021
Chulalongkorn University
Expression Of Recombinant S2 Domain Of Spike Protein Of Porcine Epidemic Diarrhea Virus, Jiraporn Sritun, Natnaree Inthong, Siriluk Jala, Sakuna Phatthanakunanan, Kaitkanoke Sirinarumitr, Preeda Lertwatcharasarakul, Theerapol Sirinarumitr
The Thai Journal of Veterinary Medicine
Porcine epidemic diarrhea virus (PEDV) is the causative agent of severe watery diarrhea in suckling piglets. PEDV outbreaks have been consistently reported in Europe, Asian countries and Thailand. The recombinant protein, especially the spike (S) protein, from field strain PEDV could be used to develop a sensitive diagnostic test or an effective vaccine. In this study, the recombinant S2 domain protein from the PEDV Thai isolate was expressed using the E. coli expression system. The full length S2 domain of the S gene of the PEDV G2b Thai isolate was amplified using a PCR technique and was cloned into the …
Identification And Characterization Of A Novel Protective Antigen, Sesec_00396 Of Streptococcus Equi Ssp. Zooepidemicus,
2021
Chulalongkorn University
Identification And Characterization Of A Novel Protective Antigen, Sesec_00396 Of Streptococcus Equi Ssp. Zooepidemicus, Li Yi, Weiping Yang, Jinpeng Li, Shaohui Wang, Yang Wang
The Thai Journal of Veterinary Medicine
Streptococcus equi ssp. zooepidemicus (SEZ) is responsible for a wide variety of infections in many animal species. Studies aimed at developing safe and effective vaccines against SEZ infections are thus of great interest. SeseC_00396 was a novel protein identified in the previous study. In this study, recombinant SeseC_00396 of SEZ was expressed and showed a strong immunoreactivity with mini-pig convalescent sera. Studies in mice have revealed that the recombinant protein induced a marked antibody response which was associated with protection against SEZ infection. The hyperimmune sera against SeseC_00396 could efficiently kill the bacteria. In addition, it was also found that …
Investigation Of Avian Polyomavirus And Psittacine Beak And Feather Disease Virus In Parrots In Taiwan,
2021
Chulalongkorn University
Investigation Of Avian Polyomavirus And Psittacine Beak And Feather Disease Virus In Parrots In Taiwan, Sheng-Chang Chung, Ching-Dong Chang, Perng-Chih Shen, Wei Lin, Chiu-Huang Ting, Hung-Yi Wu
The Thai Journal of Veterinary Medicine
This study aimed to understand the related statistics of APV and PBFDV in parrots, such as the positive percentage and the distributions of various ages, seasons, breeds and areas, in order to obtain more information on the health care of captive parrots in Taiwan today. Blood samples were collected from parrots that were household pets and from commercial aviaries in the northern (e.g. Taipei), central (e.g. Taichung) and southern (e.g. Kaohsiung) Taiwan regions randomly. Collection targets were mainly small and medium species such as Psittacula krameri and others such as Psittacus erithacus. Sixty samples were collected every month and a …
Prevalence And Risk Factors Of Haemotropic Mycoplasma Ovis Infection In Selected Smallholder Sheep And Goat Flocks In Malaysia,
2021
Chulalongkorn University
Prevalence And Risk Factors Of Haemotropic Mycoplasma Ovis Infection In Selected Smallholder Sheep And Goat Flocks In Malaysia, Bura Thlama Paul, Faez Firdaus Abdullah Jesse, Eric Lim Teik Chung, Azlan Che-Amat, Mohd Lila Mohd-Azmi
The Thai Journal of Veterinary Medicine
Outbreaks of haemotropic Mycoplasma ovis cause haemolytic anaemia with decreased production outcomes and mortality in sheep and goats worldwide but there is a lack of published data on the epidemiology of M. ovis in Malaysia's small ruminant flocks. This preliminary study investigates the prevalence and risk factors of Mycoplasma ovis infection in selected smallholder farms in Negeri Sembilan state. A total of 341 blood samples was randomly collected from 5 farms between January and December 2019. The farmers also completed a structured questionnaire to provide farm management data and environmental information. Giemsa stained blood smears were examined microscopically to detect …
Immune-Responsiveness, Performance And Blood Chemistry Of Broiler Chickens Fed Black Cumin Seed And/Or Turmeric,
2021
Chulalongkorn University
Immune-Responsiveness, Performance And Blood Chemistry Of Broiler Chickens Fed Black Cumin Seed And/Or Turmeric, Sabry M. El-Bahr, A. Al-Ankari, Saad Shousha
The Thai Journal of Veterinary Medicine
Two hundred and fifty one-day old broiler chicks were used to evaluate the effect of black seeds and/or turmeric on performance and immune response to Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV) and Infectious Bursal Disease virus (IBDV) in broiler chickens of six weeks of age. The birds were divided into five groups (50 birds in each) with 5 replicates (10 birds each). Birds in the first group were fed a basal diet and served as a control. Birds of groups 2-5 were fed a basal diet mixed with black seed (20g/ kg basal diet), turmeric (20g/ kg basal …
The Effect Of Low-Level Laser Therapy On Rat Unicortical Femoral Defect,
2021
Chulalongkorn University
The Effect Of Low-Level Laser Therapy On Rat Unicortical Femoral Defect, Latif Emrah Yanmaz, Ugur Ersoz, Sitkican Okur, Betul Apaydin Yildirim, Selim Comakli, Yavuz Selim Sağlam, Elif Dogan, Ozhan Karatas, Ferda Turgut, Zafer Okumuş
The Thai Journal of Veterinary Medicine
The aim of this study was to determine the effectiveness of low-level laser therapy (LLLT) in the healing of experimentally created unicortical defect (UCD) of rat femurs. Twenty-four adult Wister rats were randomly divided into 2 groups: UCD and UCD+LLLT. The animals were subdivided into 3 groups (each group had 4 animals) and then they sacrificed at day 8, 15 and 22 for radiological, pathological and biochemical analysis. Bone healing was significantly different between the groups at day 15 and 22 (P < 0.05), while bone formation was significantly increased in the UCD+LLLT group at day 22 compared to the UCD group (P < 0.05). On histopathological examination, significant difference was found between the UCD and UCD+LLLT groups on the 22nd day (P < 0.05) while there was no difference on the 8th and 15th day. In the UCD group, the plasma Alkaline phosphatase activity decreased on the 15th and 22nd days, Calcium on the 15th day and Phosphorus on the 8th and 22nd days compared to the UCD+LLLT group. In addition, Glutathione levels, Plasma Catalase, Glutathione Peroxidase and Superoxide Dismutase activity significantly reduced in the UCD group compared with UCD+LLLT on the 8th, 15th and 22nd day (P < 0.05). In conclusion, LLLT applied at a dose of 904µm accelerates fracture healing in UCD of a rat’s femur. However, this effect shows an increase after the 22nd day of LLLT application.
Retrospective Analysis Of The Incidence, Aetiology And Risk Factors Of Sepsis Based On Post-Mortem Examination Records In Ruminants,
2021
Chulalongkorn University
Retrospective Analysis Of The Incidence, Aetiology And Risk Factors Of Sepsis Based On Post-Mortem Examination Records In Ruminants, Batrisyia Syazana Faridon, Mohd Zamri-Saad, Annas Salleh
The Thai Journal of Veterinary Medicine
Sepsis is an important disease in both humans and animals. In animals, it is usually caused by septicaemia after infection and has not been well-studied. This article describes a retrospective analysis of sepsis cases in small and large ruminants submitted to the Post-Mortem Laboratory, Faculty of Veterinary Medicine, Universiti Putra Malaysia between the years 2007 and 2017. The incidence of sepsis was found to be at 40.5% in small ruminants and 38.6% in large ruminants. In both small and large ruminants, sepsis was mainly caused by bacterial infection, which was significantly (p<0.05) higher compared to parasitic and viral infections. Most cases of sepsis occur due to infections originating from the gastrointestinal tract and this is followed by the respiratory tract. Escherichia coli has been found to be the most common bacterial pathogen of sepsis in ruminants; significantly (p<0.05) higher compared to other bacterial pathogens like Klebsiella pneumoniae, Pasteurella sp., and Staphylococcus aureus. Sepsis involving E. coli has often resulted in pure isolation of the pathogen, thus being suggestive of a single infection. Underlying or concurrent infection is the most important risk factor leading to sepsis in ruminants, with significant difference (p<0.05) compared to other risk factors such as stress or malnutrition. Associations between the gastrointestinal system as the point of entry and sepsis caused by either E. coli or K. pneumoniae have been noted. This study provides an insight into a basic understanding of sepsis in ruminants.
Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs,
2021
Chulalongkorn University
Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
A total of 292 E. coli isolates obtained from fecal samples from pigs in Central (n = 103) and Northeastern (n = 189) provinces of Thailand were included in this study. Eighty-six E. coli isolates were phenotypically confirmed to be β- lactamases producers and screened for the presence of β-lactamase genes. The CTX-M family was most frequently identified (90.7%). The blaCTX-M-15 gene belonging to CTX-M group I (59.3%) was the most predominantly identified CTX-M genotype followed by blaCTX-M-14 (31.4%) and blaCTX-M-4 (25.6%). The blaTEM-1 gene was prevalent (75.6%). The blaCTX-M-4 and blaCTX-M-14 genes were located on conjugative plasmid. The results …
Effects Of Medium-Chain Free Fatty Acids On Performance, Some Biochemical Parameters And Meat Fatty Acids Profile Of Broiler Chickens,
2021
Chulalongkorn University
Effects Of Medium-Chain Free Fatty Acids On Performance, Some Biochemical Parameters And Meat Fatty Acids Profile Of Broiler Chickens, Mehmet Demi̇rci̇, Mehmet Başalan
The Thai Journal of Veterinary Medicine
In this study, it was aimed to investigate the effects of the addition of caprylic (octanoic, C8:0), capric (decanoic, C10:0) and lauric (dodecanoic, C12:0) acids from medium-chain free fatty acids to broiler diets. A total of 120 one-dayold male broiler chicks (Ross 308) were used and the study was conducted on 4 main groups of broilers; one control and three trials. The birds in the control group was fed an unadulterated basal diet and those in the experimental groups were fed with 0.2% of caprylic, capric and lauric acids (in addition to a basal diet) respectively. In the study, there …
Downregulation Of Kynurenine 3-Monooxygenase Inhibits Cancer Stemness-Related Genes In Both Human And Canine Breast Tumor Cell Lines,
2021
Chulalongkorn University
Downregulation Of Kynurenine 3-Monooxygenase Inhibits Cancer Stemness-Related Genes In Both Human And Canine Breast Tumor Cell Lines, Chia-Tang Chen, Chiao-Hsu Ke, Hsin-Pei Weng, Chi-Hsun Liao, Chen-Si Lin
The Thai Journal of Veterinary Medicine
Canine mammary tumors (CMT) and human breast cancer (HBC) are the most frequent cancers in both female dogs and women worldwide, and they share an urgency for the development of effective markers. Overexpression of kynurenine 3-monooxygenase (KMO), a secondary enzyme of the kynurenine pathway, has been shown to indicate poor prognosis in CMT patients. To further investigate the role of KMO in mammary tumor development, we first analyzed the significant pathways related to KMO from the Database for Annotation, Visualization and Integrated Discovery (DAVID). The results revealed that several Kyoto Encyclopedias of Genes and Genomes (KEGG) pathways might correlate with …
A Novel Custom-Made External Fixator For Treating Femur Fractures In Goats,
2021
Chulalongkorn University
A Novel Custom-Made External Fixator For Treating Femur Fractures In Goats, Hsu-Hsun Lee, Ting-Sheng Lin, Lee-Shuan Lin, Cheng-Shu Chung, Shyh-Shyan Liu, Huan-Yu Hsu, Ching-Hou Ma, Jai-Wei Lee
The Thai Journal of Veterinary Medicine
External fixation is commonly used to treat long bone fractures in goats. It provides good enough stabilization on fractures to improve healing, minimizes soft tissue injury and only causes mild obstruction of the blood supply around the wounds. However, the large size and heavy weight of traditional external fixators (TEFs) cause them leave something to be desired. In this study, the feasibility of a novel custom-made external fixator (CMEF) for treating femoral fractures in goats was evaluated. Three one-year-old Anglo-Nubian female goats were subjected to experimental femoral fractures to their left femur with a CMEF installed for fixation. To evaluate …
Left Lateral Liver Lobe Torsion In A Siamese Cat,
2021
Chulalongkorn University
Left Lateral Liver Lobe Torsion In A Siamese Cat, Dongwook Kim, Ki-Jeong Na, Dongwoo Chang, Gonhyung Kim
The Thai Journal of Veterinary Medicine
A 6-year-old neutered male Siamese cat was presented with an acute onset of vomiting, anorexia and lethargy lasting for four days. An abdominal mass was palpated on physical examination. The cat showed severe anemia, elevated liver enzymes and hypoalbuminemia. Abdominal ultrasonography revealed a suspected hepatic mass without blood flow within the affected region. Computed tomography was performed to better visualize the abdominal cavity and blood flow. Prompt diagnosis of torsion of the left lateral liver lobe was made and immediate surgery for liver lobectomy was performed. Histopathological examination revealed no evidence of neoplasia. The cat remained clinically normal during the …
The Use Of Human Suprapubic Bladder Catheter To Manage Obstructive Urolithiasis In A Saanen Buck,
2021
Chulalongkorn University
The Use Of Human Suprapubic Bladder Catheter To Manage Obstructive Urolithiasis In A Saanen Buck, Nurul Hayah Khairuddin, Mark Wen Han Hiew, Syahirah Ahmad Affandi, Azim Salahudin Muhamad, Nur Asyikin Haron, Rozanaliza Radzi, Chen Hui Cheng, Ganesh Vythilingam
The Thai Journal of Veterinary Medicine
A three-year-old Saanen buck presented with a complaint of stranguria for 24 hours. A physical examination revealed that the perineal urethra was turgid, swollen, and tender, and the urinary bladder was distended. The goat was diagnosed with obstructive urolithiasis. A percutaneous tube cystotomy technique was performed to relieve progressive urinary bladder distension. Using a human suprapubic urinary bladder catheter system, the technique was performed twice due to the recurrence of the blocked urethra. Following drainage of the urine, all vital parameters returned to normal. After surgical cystotomy, an intraoperative observation revealed the urinary bladder was filled with white sandy urolith, …
Radiographic And Ultrasonographic Appearances Of Chronic Tenosynovitis Of The Lateral Extensor Tendon At The Right Tarsus In An Argentinian Polo Pony,
2021
Chulalongkorn University
Radiographic And Ultrasonographic Appearances Of Chronic Tenosynovitis Of The Lateral Extensor Tendon At The Right Tarsus In An Argentinian Polo Pony, Metha Chanda, Ananya Pongpradit, Wutthiwong Theerapan, Attapon Kamlangdee, Chanikarn Puangthong, Thapana Jarutummasiri
The Thai Journal of Veterinary Medicine
A 19-year-old polo pony presented with mild lameness alongside a lateral protuberance of the right tarsus. Radiographic findings revealed various defects, including an osteochondral lesion of the medial trochlear ridge of the talus, a sclerotic lesion of the tarsometatarsal joint, a radiolucent area on the distal border of the fourth tarsal bone and an enthesophyte at the proximolateral aspect of the metatarsal bone. Ultrasonography demonstrated the interesting finding of synovial sheath thickening of the lateral extensor tendon with synovial fluid accumulation. In addition, the presence of neutrophils and a granular mucinous background, according to cytological analysis of the fluid, indicated …
Case Report: Peripheral Iridectomy And Sclerectomy For Uveitic Glaucoma In A Labrador Retriever,
2021
Chulalongkorn University
Case Report: Peripheral Iridectomy And Sclerectomy For Uveitic Glaucoma In A Labrador Retriever, Natthanet Sritrakoon, Aree Thayananuphat
The Thai Journal of Veterinary Medicine
A 4-year-old male Labrador retriever dog presented with a history of conjunctivitis OU and mild buphthalmos OS for one week. Ophthalmic examination revealed corneal edema, conjunctivitis, iritis, aqueous flare, posterior synechia, fibrin on the anterior lens capsule, increased intraocular pressure (IOP =30 mmHg OD; IOP = 48 mmHg OS), weak positive menace response, brisk positive dazzle reflex and negative pupillary light reflex OU. The iridocorneal angle could not be visualized due to corneal edema. Physical examination, hematology and blood chemistry results were normal. Both eyes were diagnosed as having glaucoma and uveitis. Topical prednisolone acetate, atropine sulfate, brinzolamide and timolol …
