Veterinary Medicine Commons^™

Sex And Nest Type Influence Avian Blood Parasite Prevalence In A High-Elevation Bird Community, Marina D. Rodriguez, Paul F. Doherty, Antoinette J. Piaggio, Kathryn P. Huyvaert

United States Department of Agriculture Wildlife Services: Staff Publications

Background: The prevalence of avian haemosporidian parasites and the factors influencing infection in the Colorado Rocky Mountains are largely unknown. With climate change expected to promote the expansion of vector and avian blood parasite distributions, baseline knowledge and continued monitoring of the prevalence and diversity of these parasites is needed.

Methods: Using an occupancy modeling framework, we conducted a survey of haemosporidian parasite species infecting an avian community in the Colorado Rocky Mountains in order to estimate the prevalence and diversity of blood parasites and to investigate species-level and individual-level characteristics that may influence infection.

Results: We estimated the prevalence …

Potential Use For Serosurveillance Of Feral Swine To Map Risk For Anthrax Exposure, Texas, Usa, Rachel M. Maison, Courtney F. Pierce, Izabela K. Ragan, Vienna R. Brown, Michael J. Bodenchuk, Richard A. Bowen, Angela M. Bosco-Lauth

United States Department of Agriculture Wildlife Services: Staff Publications

Anthrax is a disease of concern in many mammals, including humans. Management primarily consists of prevention through vaccination and tracking clinical-level observations because environmental isolation is laborious and bacterial distribution across large geographic areas diffi cult to confi rm. Feral swine (Sus scrofa) are an invasive species with an extensive range in the southern United States that rarely succumbs to anthrax. We present evidence that feral swine might serve as biosentinels based on comparative seroprevalence in swine from historically defi ned anthrax-endemic and non–anthraxendemic regions of Texas. Overall seropositivity was 43.7% (n = 478), and logistic regression revealed county endemicity …

Successful Treatment Of Demodicosis Due To Demodex Cati In A Cat Using Spot – On Formulation Of Fluralaner, Piyarat Chansiripornchai, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

This case report describes the successful use of fluralaner in treating demodicosis caused by Demodex cati in a cat. A 4.2 kg 10 – year – old spayed female Siamese short – haired cat was presented with a 5 – month history of severe dermatitis. The cat had been treated at previous animal hospitals with a variety of drugs such as antimicrobials, glucocorticoids and ectoparasiticides on separate occasions but the clinical signs remained severe. Microscopic examination of skin samples revealed the presence of D. cati. The cat was treated with a single dose of spot – on formulation of fluralaner …

Calcinosis Circumscripta Associated With Surgical Site Of The Right Femur In A Belgian Sheepdog: Imaging Diagnosis And Surgical Treatment, Naparee Srisowanna, Kanittha Darawiroj, Somporn Techangamsuwan, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

A nine-month, intact male, Belgian Sheepdog was presented to the hospital due to a history of rapidly progressive growth of a solitary calcified mass at the right hip area. The dog has a history of an internal implant fixation for the right femoral fracture at 5 months ago. Physical examination revealed a dome shape, hard consistency, non-movable, painless, subcutaneous mass with size 5x6 cm. Radiography and ultrasonography showed a well-circumscribed, calcified soft tissue mass at craniolateral aspect of the right proximal femur without cortical bone reaction. Computed tomography (CT) scan was not performed in this case because metal fixation was …

Diagnosis Of Toothpick-Induced Chronic Intramuscular Abscess Using Fistulography In A Dog, Pei-Chun Hsieh, Kuan-Sheng Chen, Wei-Ming Lee, Wei-Yau Shia

The Thai Journal of Veterinary Medicine

A ten-year-old, 16.7 kg mixed-breed dog presented with chronic lameness and delayed healing of discharging wounds in the right pelvic limb following a puncture injury for approximately 6 months. Debridement surgery had been performed, but the tissue infection was persistent. Plain radiographic examination showed neither increased soft tissue opacity nor foreign body (FB) existence near the discharging wound of the right pelvic limb. After the second debridement surgery, closed drainage tubes were placed, and susceptible antibiotic therapy was prescribed in an attempt to control the persistent infection, but the fluid draining from the wound was still purulent. Therefore, fistulography was …

The Influence Of Intranasal Peste Des Petits Ruminants Vaccine Application Methods On The Induction Of Immune Responses In Goats: Clinicopathological And Immunohistochemical Findings, Chukwunonso Kenechukwu Ezeasor, Shodeinde Vincent Shoyinka, Benjamin Obukowho E Mikpe, Ikechukwu John Udeani, Nick Nwankpa, Charles Sanne Bodjo, Mohd Yusoff Sabri

The Thai Journal of Veterinary Medicine

Intranasal administration of peste des petits ruminants (PPR) vaccine may induce mucosal and systemic immune responses in goats but little is known about the influence of the vaccine application methods. This study reports the influence of two different intranasal vaccine application methods on the immune responses in goats following intranasal PPR vaccination. Twenty, male west African dwarf goats were divided into 4 groups (n=5). Groups A and B were vaccinated intranasally (IN) with live attenuated PPR vaccine (Nigeria 75/1) by either nasal dropper (Group A) or nasal spray (Group B) methods and compared with the subcutaneous route of vaccination (Group …

Diagnostic Utility Of Basal Serum T4 And Tsh Concentrations In Dogs With Hypothyroidism Confirmed By Tsh Stimulation Test, Minkeun Shin, Dohee Lee, Taesik Yun, Yoonhoi Koo, Byeong-Teck Kang, Mhan-Pyo Yang, Hakhyun Kim

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate the diagnostic performance of a basal serum thyroxine (T4) and thyrotropin (TSH) combination test for the diagnosis of canine hypothyroidism in clinical practice. It was hypothesized that the usefulness of basal T4 and TSH combination test in dogs with suspected non-thyroidal illness syndrome would be different with that in previous studies. The data of this study were searched for dogs who underwent both TSH stimulation test and basal serum T4 and TSH combination test. Forty-four cases were classified into either of three groups (hypothyroid, intermediate, or euthyroid) according to the results of …

The Alterations Of Fecal Microbiota In Dogs With Acute Diarrhea, Thailand, Jeerawat Soonthornsit, Natharin Ngamwongsatit, Panpanga Sangsuriya, Nlin Arya

The Thai Journal of Veterinary Medicine

Acute diarrhea is a common clinical sign in dogs which the pathogenesis has associated with altered gut microbiome compositions and influenced by diet, environment, and medication. However, the impact of gut microbiome on dog health has still not been well elucidated in Thailand. This study aims to investigate the alterations of the fecal microbiome in dogs with diarrhea (<3 days) compared to healthy dogs. Fresh feces were collected once from each dog, and DNA extraction was performed following the QIAamp DNA stool mini kit protocol. The 16s rRNA gene, then, amplified and analyzed by the next-generation DNA sequencing technology. The results showed a significant reduction of the evenness and Shannon index in dogs with the diarrhea group. The most dominant phylum in healthy dogs, Firmicutes, was markedly declined. Additionally, significant decreases in bacterial abundances were observed in twelve genera. To the best of our knowledge, seven of them were first described in their alterations including Clostridium sensu stricto 1, Lachnospiraceae NKA4136 group, Erysipelatoclostridium, Tyzzerella 3, Alloprevotella and Slackia. Therefore, these findings disclose the new possible biomarkers for intestinal diseases and the effect of ordinary diets on the fecal microbiome in dog health, Thailand.

Comparison Of The Effects Of Different Thawing Methods On Post-Thaw Sperm Characteristics Of Buffalo Bull Semen, Eser Akal, Murat Selçuk, Burcu Esi̇n, Melih Akar, Cumali Kaya

The Thai Journal of Veterinary Medicine

Application proper procedures for thawing of cryopreserved sperm are required for successful artificial insemination. The study was aimed to compare the effects of watery and dry thawing systems on motility, acrosome integrity, plasma membrane integrity, morphologic and kinematic characteristics of buffalo bull spermatozoa. Anatolian Buffalo bull spermatozoa, cryopreserved in volumes of 0.25 mL straws, were thawed by using 1) watery thawing system; a total of 10 straws were thawed in a water bath at 37 °C for 30 sec and 2) dry thawing system; a total of 10 straws were thawed in a dry thawing device at 37 °C for …

Evaluation Of The Hematological And Serum Protein Profiles Of Blood Parasite Coinfection In Naturally Infected Dogs, Sariya Asawakarn, Sirakarnt Dhitavat, Piyanan Taweethavonsawat

The Thai Journal of Veterinary Medicine

Canine vector-borne diseases (CVBDs) are prevalent worldwide, including Thailand. Vectors can transmit more than one pathogen, and coinfections between blood parasites and/or filariae have been reported. Pathogenesis may affect the health status of infected dogs. Hematological, blood chemistry, and serum protein profile abnormalities can be used to screen for underlining causes of CVBDs. The aim of this study is to analyze the hematological and serum protein profiles as well as CRP in CVBD coinfection cases. In this study, 22 blood parasite coinfection cases are examined and classified into two groups: blood parasite coinfection (group 1: n = 16) and blood …

A Seroepidemiological Investigation On Major Viral And Bacterial Pathogens In Small-Scale Chicken Flocks In The Mekong Delta Region Of Vietnam, Nguyen Thi Bich Van, Nguyen Van Cuong, Nguyen Thi Nhung, Nguyen Thi Phuong Yen, Le Vy, Bach Tuan Kiet, Nguyen Van Hoang, Vo Be Hien, Ho Thi Viet Thu, Niwat Chansiripornchai, Guy E. Thwaites, Marc Choisy, Juan Carrique-Mas

The Thai Journal of Veterinary Medicine

Small-scale commercial chicken farming represents an important source of income to rural households in the Mekong Delta region of Vietnam. A wide range of vaccines are currently administered to flocks, but is not based on empirical knowledge on pathogen circulation. We collected serum samples and vaccination data from a random sample of 267 small-scale native chicken flocks in Dong Thap to determine serological profiles by ELISA against 8 pathogens: Avian Influenza (AI), Chicken Anaemia virus (CAV), Infectious Bursal Disease (IBD) virus, Infectious Bronchitis virus (IBV), Newcastle Disease virus (NDV), O. rhinotracheale (ORT), P. multocida (PM) and M. gallisepticum (MG). The …

Bacterial Counts And Prevalence Of Salmonella And Extended-Spectrum Β-Lactamase Producing Bacteria In Prawn And Shrimp Imported Into Japan, Yukio Morita, Su Myo Thwe, Satoshi Morita, Sumalee Boonmar

The Thai Journal of Veterinary Medicine

The prevalence of Non-typhoidal Salmonella (NTS) and extended-spectrum β-lactamase (ESBL) producing bacteria was assessed in samples of imported prawn and shrimp obtained from retail markets in Japan. The overall prevalence of NTS and ESBL producing bacteria was 4%, and 14%, respectively. We identified 4 NTS isolates, including S. enterica subspecies salamae, and 3 serovars of S. enterica subspecies enterica, including Weltevreden, Abony, and Stanley. ESBL producing bacteria were isolated from 14 of 100 samples, thirteen of which harbored Stenotrophomonas maltophilia and that harbored Chryseobacterium indologenes. Among the S. maltophilia-positive samples, six had been imported from India, five samples came from …

Estimation Of Percentage Of Morbidity And Mortality Due To Avian Influenza (H9) At Commercial Poultry Layer Farms Of Lahore, Afeefa Shafiq, Aisha Arshad, Zafar-Ul-Ahsan Qurashi, Zain Khalid, Noor Ul Huda, Muhammad Noman, Muhammad Ilyas, Hamad Bin Rashid, Asim Khalid Mehmood, Saima Hasan, Quratul Ain, Sobia Amir, Sheraz Shahid, Shakera Sadiq, Mamoona Chaudhry

The Thai Journal of Veterinary Medicine

The current study was aimed at the estimation of the percentage of morbidity, mortality and potential risk factors associated with the avian influenza virus (AIV) H9 at commercial layer farms of District Lahore, Pakistan. Samples collected included blood samples and tracheal/oropharyngeal swabs from sick birds and tissue samples (trachea & lungs) of dead birds. Sera were tested by Hemagglutination Inhibition Test (HI) using the positive control antigen of H9. Tracheal/oropharyngeal swabs and tissue samples were processed by real-time RT-PCR (rRT-PCR). Out of 50 layer farms, 16 farms were positive for antibody titer against the AIV H9. Out of a total …

Effects Of Senktide On Kisspeptin/Neurokinin B/Dynorphin Peptide Mrna Expression And Luteinizing Hormone Secretion In Fasted Female Goats: A Pilot Study, Pongpak Intaravichai, Somchai Sajapitak, Sittipoj Krasaesub, Tatchapon Mongkonwattanaporn, Theera Rukkwamsuk

The Thai Journal of Veterinary Medicine

This pilot study aimed to examine KNDy peptide mRNA (Kiss1/Tac3/Pdyn) expression and to determine the effects of peripheral neurokinin 3 receptor agonist (senktide) administration on the ARC Kiss1 mRNA expression and LH secretion that was affected by fasting condition in intact female goats during the luteal phase of the estrus cycle. During the mid to late luteal phase of the estrus cycle, the goats were randomly divided into three groups; the fed group (n = 2), which was fed ad libitum throughout the experimental period, the fasted group (n = 2) and the fasted + senktide group (n = 2) …

Surgical Correction Of Bilateral Soft Palate Hypoplasia Using Soft Palate Hinged-Flap And Bilateral Buccal Mucosal Rotation Flaps In A Dog, Wongsuda Yala, Chaiyasit Suksawakhon, Wanchart Yippaditr, Julaluk Kruafu

The Thai Journal of Veterinary Medicine

A 3-month-old 13 kg intact female American pit bull dog was presented with a history of chronic sneezing, nasal discharge, oronasal reflux, and aspiration with food and water. Oral examination under general anesthesia revealed a shortened soft palate extended caudally from the hard palate. As a result, bilateral soft palate hypoplasia was diagnosed. The dog was operated upon by using soft palate hinged-flap combined with bilateral buccal mucosal rotation flaps techniques. Ten days after surgery, the dog was eating well and the nasal discharge was resolved. Oral examination showed that the new soft palate had healed well. A follow-up after …

Impact Of Fish Oil On Intestinal Permeability, Inflammation, And Performance In Swine, Anthony Fernando Alberto

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Our research examined the effects of fish oil supplementation on intestinal permeability, systemic inflammation and performance in piglets. An lipopolysaccharide (LPS) challenge was done to stimulate the synthesis and release of the cytokines, in an effort to mimic the immune response that piglets would have when facing stress or pathogen challenge. Fish oil increased feed intake but did not affect growth when compared to control. Total fatty acid digestibility increased by 6% when fish oil was included in the diet. Also, we observed a 16% increase on 16-carbon fatty acids digestibility. Fish oil did not affect the plasma …

Genetic Characterization Of Banteng (Bos Javanicus) Populations In Thailand For Conservation, Sirinart Chaichanathong, Worata Klinsawat, Manakorn Sukmak, Apichaya Sakulthai, Worawidh Wajjwalku, Supaphen Sripiboon, Nongnid Kaolim, Seree Nakbhun, Burachat Tunpradit, Tarasak Nipanunt, Wanlaya Tipkantha, Marnoch Yindee, Nikorn Thongtip

The Thai Journal of Veterinary Medicine

Banteng (Bos javanicus), an endangered, wild ungulate, plays a major role in seed dispersal and as a prey animal in Thailand. The population of wild banteng is threatened by poaching and habitat losses. Captive breeding management of banteng has been established and reintroduction of banteng has been successful in some areas. This study investigated the genetic variation of wild and captive banteng, based on mitochondrial DNA (mtDNA) and the Y-chromosome. The mtDNA analysis revealed three novel maternal haplotypes. The Y-chromosome analysis showed two Y-chromosome haplotypes based on the SRY region in the Thai population. This region may be useful as …

Evaluating The Effects Of Prunus Laurocerasus Seed, Fruit And Leaf Extracts On Hyperglycaemia, Insulin Sensitivity And Anti-Oxidative Activities In Experimental Diabetes In Rat, Hamit Uslu, Gözde Atila Uslu

The Thai Journal of Veterinary Medicine

Although insulin resistance is widely accepted as one of the main characteristics of type 2 diabetes, there are studies suggesting that there is a link between the development of type 1 diabetes and insulin resistance in recent years. Therefore, in this study, it was aimed to investigate the effects of Prunus laurocerasus (PL) seeds, fruit and leaf extracts on hyperglycemia, adiponectin and irisin levels which are known to be effective in the mechanisms of insulin sensitivity, and oxidative stress in rats with type 1 diabetes. The study groups were as follows: normoglycaemic (NC), diabetic control (DC), diabetic + seed extract …

Prevalence Of Shiga Toxin-Producing Escherichia Coli In Buffaloes On Smallholdings In Coastal Area In Bangladesh, Md Shahriar Mannan, Farzana Rabbi, Md Gholam Kibria, Sirazul Islam, Md. Bayzid, Zamila Bueaza Bupasha, Md Zohorul Islam, Md Samun Sarker, Paritosh Kumar Biswas

The Thai Journal of Veterinary Medicine

Shiga toxin-producing Escherichia coli (STEC) is an important causal agent of food borne diseases. Although the presence of STEC has been reported in cattle, goat, and sheep in previous studies in Bangladesh, there is seemingly no information on sorbitol non-fermenting STEC (SN-F STEC) carriage in buffalo. We aimed to assess the prevalence of SN-F STEC and to identify common virulence genes in SN-F STEC strains from apparently healthy buffaloes on smallholdings in coastal area in Bangladesh. Total 308 buffaloes on smallholdings in Patenga and Anwara Upazila, Chattogram division, Bangladesh were randomly sampled by collecting a swab from the recto-anal junction …

Antifungal Activity Of Green Sulfur Nanoparticles Synthesized Using Catharanthus Roseus Extract Against Microsporum Canis, Akarapon Chantongsri, Patchara Phuektes, Glenn N. Borlace, Jareerat Aiemsaard

The Thai Journal of Veterinary Medicine

Elemental sulfur has been used for a long time to treat superficial mycoses in both medical and veterinary practices, but effective treatment requires high concentrations of sulfur. Encapsulation of sulfur in nanoparticles can potentially allow the delivery of high concentrations of sulfur to infections. Sulfur nanoparticles were synthesized from Catharanthus roseus extract and sodium sulfide at various pH conditions (4, 6, and 7). Particle identification and characterizations were performed by X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), and dynamic light scattering analysis. Broth microdilution and time-kill assays were used to determine the antifungal effect against Microsporum canis DMST29297. The …