The Alterations Of Fecal Microbiota In Dogs With Acute Diarrhea, Thailand,
2021
Chulalongkorn University
The Alterations Of Fecal Microbiota In Dogs With Acute Diarrhea, Thailand, Jeerawat Soonthornsit, Natharin Ngamwongsatit, Panpanga Sangsuriya, Nlin Arya
The Thai Journal of Veterinary Medicine
Acute diarrhea is a common clinical sign in dogs which the pathogenesis has associated with altered gut microbiome compositions and influenced by diet, environment, and medication. However, the impact of gut microbiome on dog health has still not been well elucidated in Thailand. This study aims to investigate the alterations of the fecal microbiome in dogs with diarrhea (<3 days) compared to healthy dogs. Fresh feces were collected once from each dog, and DNA extraction was performed following the QIAamp DNA stool mini kit protocol. The 16s rRNA gene, then, amplified and analyzed by the next-generation DNA sequencing technology. The results showed a significant reduction of the evenness and Shannon index in dogs with the diarrhea group. The most dominant phylum in healthy dogs, Firmicutes, was markedly declined. Additionally, significant decreases in bacterial abundances were observed in twelve genera. To the best of our knowledge, seven of them were first described in their alterations including Clostridium sensu stricto 1, Lachnospiraceae NKA4136 group, Erysipelatoclostridium, Tyzzerella 3, Alloprevotella and Slackia. Therefore, these findings disclose the new possible biomarkers for intestinal diseases and the effect of ordinary diets on the fecal microbiome in dog health, Thailand.
Comparison Of The Effects Of Different Thawing Methods On Post-Thaw Sperm Characteristics Of Buffalo Bull Semen,
2021
Chulalongkorn University
Comparison Of The Effects Of Different Thawing Methods On Post-Thaw Sperm Characteristics Of Buffalo Bull Semen, Eser Akal, Murat Selçuk, Burcu Esi̇n, Melih Akar, Cumali Kaya
The Thai Journal of Veterinary Medicine
Application proper procedures for thawing of cryopreserved sperm are required for successful artificial insemination. The study was aimed to compare the effects of watery and dry thawing systems on motility, acrosome integrity, plasma membrane integrity, morphologic and kinematic characteristics of buffalo bull spermatozoa. Anatolian Buffalo bull spermatozoa, cryopreserved in volumes of 0.25 mL straws, were thawed by using 1) watery thawing system; a total of 10 straws were thawed in a water bath at 37 °C for 30 sec and 2) dry thawing system; a total of 10 straws were thawed in a dry thawing device at 37 °C for …
Antifungal Activity Of Green Sulfur Nanoparticles Synthesized Using Catharanthus Roseus Extract Against Microsporum Canis,
2021
Chulalongkorn University
Antifungal Activity Of Green Sulfur Nanoparticles Synthesized Using Catharanthus Roseus Extract Against Microsporum Canis, Akarapon Chantongsri, Patchara Phuektes, Glenn N. Borlace, Jareerat Aiemsaard
The Thai Journal of Veterinary Medicine
Elemental sulfur has been used for a long time to treat superficial mycoses in both medical and veterinary practices, but effective treatment requires high concentrations of sulfur. Encapsulation of sulfur in nanoparticles can potentially allow the delivery of high concentrations of sulfur to infections. Sulfur nanoparticles were synthesized from Catharanthus roseus extract and sodium sulfide at various pH conditions (4, 6, and 7). Particle identification and characterizations were performed by X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), and dynamic light scattering analysis. Broth microdilution and time-kill assays were used to determine the antifungal effect against Microsporum canis DMST29297. The …
Potential Risk Factors For Brachyspira Hyodysenteriae, Lawsonia Intracellularis And Salmonella Spp. Infection And Their Prevalence In Commercial Swine Farms In Thailand,
2021
Chulalongkorn University
Potential Risk Factors For Brachyspira Hyodysenteriae, Lawsonia Intracellularis And Salmonella Spp. Infection And Their Prevalence In Commercial Swine Farms In Thailand, Morakot Nuntapaitoon, Sawang Katedangsakulwut, Rachod Tantilertcharoen, Napawan Bunpapong, Nanthiya Iampraphat, Suphadtra Therarachatamongkol, Jeremy Gatine
The Thai Journal of Veterinary Medicine
The aims of the present study were to evaluate the prevalence for Brachyspira hyodysenteriae, Lawsonia intracellularis and Salmonella spp. in pig feces and to identify their risk factors in 20 commercial swine farms in Thailand. The cross-sectional study was carried out from 20 farrow-to-finishing commercial swine farms in part of Thailand including the north (n=5), northeast (n=4), east (n=5), west (n=4) and south (n=1). A total of 589 fecal samples were randomly collected from 12 (n=200), 16 (n=196) and 20 (n=193) aged a week-old in each farm. All samples were analyzed for the detection of B. hyodysenteriae, L. intracellularis and …
Evaluation Of The Hematological And Serum Protein Profiles Of Blood Parasite Coinfection In Naturally Infected Dogs,
2021
Chulalongkorn University
Evaluation Of The Hematological And Serum Protein Profiles Of Blood Parasite Coinfection In Naturally Infected Dogs, Sariya Asawakarn, Sirakarnt Dhitavat, Piyanan Taweethavonsawat
The Thai Journal of Veterinary Medicine
Canine vector-borne diseases (CVBDs) are prevalent worldwide, including Thailand. Vectors can transmit more than one pathogen, and coinfections between blood parasites and/or filariae have been reported. Pathogenesis may affect the health status of infected dogs. Hematological, blood chemistry, and serum protein profile abnormalities can be used to screen for underlining causes of CVBDs. The aim of this study is to analyze the hematological and serum protein profiles as well as CRP in CVBD coinfection cases. In this study, 22 blood parasite coinfection cases are examined and classified into two groups: blood parasite coinfection (group 1: n = 16) and blood …
A Seroepidemiological Investigation On Major Viral And Bacterial Pathogens In Small-Scale Chicken Flocks In The Mekong Delta Region Of Vietnam,
2021
Chulalongkorn University
A Seroepidemiological Investigation On Major Viral And Bacterial Pathogens In Small-Scale Chicken Flocks In The Mekong Delta Region Of Vietnam, Nguyen Thi Bich Van, Nguyen Van Cuong, Nguyen Thi Nhung, Nguyen Thi Phuong Yen, Le Vy, Bach Tuan Kiet, Nguyen Van Hoang, Vo Be Hien, Ho Thi Viet Thu, Niwat Chansiripornchai, Guy E. Thwaites, Marc Choisy, Juan Carrique-Mas
The Thai Journal of Veterinary Medicine
Small-scale commercial chicken farming represents an important source of income to rural households in the Mekong Delta region of Vietnam. A wide range of vaccines are currently administered to flocks, but is not based on empirical knowledge on pathogen circulation. We collected serum samples and vaccination data from a random sample of 267 small-scale native chicken flocks in Dong Thap to determine serological profiles by ELISA against 8 pathogens: Avian Influenza (AI), Chicken Anaemia virus (CAV), Infectious Bursal Disease (IBD) virus, Infectious Bronchitis virus (IBV), Newcastle Disease virus (NDV), O. rhinotracheale (ORT), P. multocida (PM) and M. gallisepticum (MG). The …
Bacterial Counts And Prevalence Of Salmonella And Extended-Spectrum Β-Lactamase Producing Bacteria In Prawn And Shrimp Imported Into Japan,
2021
Chulalongkorn University
Bacterial Counts And Prevalence Of Salmonella And Extended-Spectrum Β-Lactamase Producing Bacteria In Prawn And Shrimp Imported Into Japan, Yukio Morita, Su Myo Thwe, Satoshi Morita, Sumalee Boonmar
The Thai Journal of Veterinary Medicine
The prevalence of Non-typhoidal Salmonella (NTS) and extended-spectrum β-lactamase (ESBL) producing bacteria was assessed in samples of imported prawn and shrimp obtained from retail markets in Japan. The overall prevalence of NTS and ESBL producing bacteria was 4%, and 14%, respectively. We identified 4 NTS isolates, including S. enterica subspecies salamae, and 3 serovars of S. enterica subspecies enterica, including Weltevreden, Abony, and Stanley. ESBL producing bacteria were isolated from 14 of 100 samples, thirteen of which harbored Stenotrophomonas maltophilia and that harbored Chryseobacterium indologenes. Among the S. maltophilia-positive samples, six had been imported from India, five samples came from …
Activation-Induced Cell Death Of Nk Cells In Canine Atopic Dermatitis Revealed By Droplet-Sequencing Data Of Peripheral Blood Mononuclear Cells,
2021
Chulalongkorn University
Activation-Induced Cell Death Of Nk Cells In Canine Atopic Dermatitis Revealed By Droplet-Sequencing Data Of Peripheral Blood Mononuclear Cells, Natthaporn Kuendee, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
The Thai Journal of Veterinary Medicine
Atopic dermatitis (AD) is a mutual allergic skin disease between humans and dogs manifesting varieties of similarities in their immune responses to the disease. Recently, activation-induced cell death (AICD) of NK cells was acknowledged as a novel disrupted immunoregulation in human AD. To gain insight into its plausible commotion in canine AD, NK cell expression profiles extracted from PBMC Drop-Sequencing (Drop-Seq) data of normal and AD dogs were analyzed along with human NK cell profiles of healthy and AD patients previously reported. According to the results, a total of 2,689 differentially expressed genes (DEG) between healthy and AD conditions (P≤0.0001) …
Estimation Of Percentage Of Morbidity And Mortality Due To Avian Influenza (H9) At Commercial Poultry Layer Farms Of Lahore,
2021
Chulalongkorn University
Estimation Of Percentage Of Morbidity And Mortality Due To Avian Influenza (H9) At Commercial Poultry Layer Farms Of Lahore, Afeefa Shafiq, Aisha Arshad, Zafar-Ul-Ahsan Qurashi, Zain Khalid, Noor Ul Huda, Muhammad Noman, Muhammad Ilyas, Hamad Bin Rashid, Asim Khalid Mehmood, Saima Hasan, Quratul Ain, Sobia Amir, Sheraz Shahid, Shakera Sadiq, Mamoona Chaudhry
The Thai Journal of Veterinary Medicine
The current study was aimed at the estimation of the percentage of morbidity, mortality and potential risk factors associated with the avian influenza virus (AIV) H9 at commercial layer farms of District Lahore, Pakistan. Samples collected included blood samples and tracheal/oropharyngeal swabs from sick birds and tissue samples (trachea & lungs) of dead birds. Sera were tested by Hemagglutination Inhibition Test (HI) using the positive control antigen of H9. Tracheal/oropharyngeal swabs and tissue samples were processed by real-time RT-PCR (rRT-PCR). Out of 50 layer farms, 16 farms were positive for antibody titer against the AIV H9. Out of a total …
Effects Of Senktide On Kisspeptin/Neurokinin B/Dynorphin Peptide Mrna Expression And Luteinizing Hormone Secretion In Fasted Female Goats: A Pilot Study,
2021
Chulalongkorn University
Effects Of Senktide On Kisspeptin/Neurokinin B/Dynorphin Peptide Mrna Expression And Luteinizing Hormone Secretion In Fasted Female Goats: A Pilot Study, Pongpak Intaravichai, Somchai Sajapitak, Sittipoj Krasaesub, Tatchapon Mongkonwattanaporn, Theera Rukkwamsuk
The Thai Journal of Veterinary Medicine
This pilot study aimed to examine KNDy peptide mRNA (Kiss1/Tac3/Pdyn) expression and to determine the effects of peripheral neurokinin 3 receptor agonist (senktide) administration on the ARC Kiss1 mRNA expression and LH secretion that was affected by fasting condition in intact female goats during the luteal phase of the estrus cycle. During the mid to late luteal phase of the estrus cycle, the goats were randomly divided into three groups; the fed group (n = 2), which was fed ad libitum throughout the experimental period, the fasted group (n = 2) and the fasted + senktide group (n = 2) …
Subtyping Of Blastocystis Infection In Common Quails (Coturnix Coturnix) In Xinxiang City Of China,
2021
Chulalongkorn University
Subtyping Of Blastocystis Infection In Common Quails (Coturnix Coturnix) In Xinxiang City Of China, Xuehan Liu, Xuefeng Yang, Zhixing An, Yaming Ge, Rongjun Wang, Haiju Dong, Huili Zhu
The Thai Journal of Veterinary Medicine
Blastocystis is a unicellular anaerobic intestinal protist primarily of various animal species worldwide, and has been reported to inhabit humans as well. Blastocystis infections and subtype identification have been documented in China, although infections by the protist in quail remain unknown. Herein, a total of 128 fecal samples were collected from captive quails (Coturnix coturnix) in Xinxiang city, central China, and tested for the prevalence and genetic characteristics of Blastocystis. Ten (7.8%) samples were positive by PCR amplification and sequencing the barcode regeion of the SSU rRNA gene. Sequence analysis showed that ST7 was the predominant subtype with max identity …
Surgical Correction Of Bilateral Soft Palate Hypoplasia Using Soft Palate Hinged-Flap And Bilateral Buccal Mucosal Rotation Flaps In A Dog,
2021
Chulalongkorn University
Surgical Correction Of Bilateral Soft Palate Hypoplasia Using Soft Palate Hinged-Flap And Bilateral Buccal Mucosal Rotation Flaps In A Dog, Wongsuda Yala, Chaiyasit Suksawakhon, Wanchart Yippaditr, Julaluk Kruafu
The Thai Journal of Veterinary Medicine
A 3-month-old 13 kg intact female American pit bull dog was presented with a history of chronic sneezing, nasal discharge, oronasal reflux, and aspiration with food and water. Oral examination under general anesthesia revealed a shortened soft palate extended caudally from the hard palate. As a result, bilateral soft palate hypoplasia was diagnosed. The dog was operated upon by using soft palate hinged-flap combined with bilateral buccal mucosal rotation flaps techniques. Ten days after surgery, the dog was eating well and the nasal discharge was resolved. Oral examination showed that the new soft palate had healed well. A follow-up after …
Successful Treatment Of Demodicosis Due To Demodex Cati In A Cat Using Spot – On Formulation Of Fluralaner,
2021
Chulalongkorn University
Successful Treatment Of Demodicosis Due To Demodex Cati In A Cat Using Spot – On Formulation Of Fluralaner, Piyarat Chansiripornchai, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
This case report describes the successful use of fluralaner in treating demodicosis caused by Demodex cati in a cat. A 4.2 kg 10 – year – old spayed female Siamese short – haired cat was presented with a 5 – month history of severe dermatitis. The cat had been treated at previous animal hospitals with a variety of drugs such as antimicrobials, glucocorticoids and ectoparasiticides on separate occasions but the clinical signs remained severe. Microscopic examination of skin samples revealed the presence of D. cati. The cat was treated with a single dose of spot – on formulation of fluralaner …
Calcinosis Circumscripta Associated With Surgical Site Of The Right Femur In A Belgian Sheepdog: Imaging Diagnosis And Surgical Treatment,
2021
Chulalongkorn University
Calcinosis Circumscripta Associated With Surgical Site Of The Right Femur In A Belgian Sheepdog: Imaging Diagnosis And Surgical Treatment, Naparee Srisowanna, Kanittha Darawiroj, Somporn Techangamsuwan, Nan Choisunirachon
The Thai Journal of Veterinary Medicine
A nine-month, intact male, Belgian Sheepdog was presented to the hospital due to a history of rapidly progressive growth of a solitary calcified mass at the right hip area. The dog has a history of an internal implant fixation for the right femoral fracture at 5 months ago. Physical examination revealed a dome shape, hard consistency, non-movable, painless, subcutaneous mass with size 5x6 cm. Radiography and ultrasonography showed a well-circumscribed, calcified soft tissue mass at craniolateral aspect of the right proximal femur without cortical bone reaction. Computed tomography (CT) scan was not performed in this case because metal fixation was …
Diagnosis Of Toothpick-Induced Chronic Intramuscular Abscess Using Fistulography In A Dog,
2021
Chulalongkorn University
Diagnosis Of Toothpick-Induced Chronic Intramuscular Abscess Using Fistulography In A Dog, Pei-Chun Hsieh, Kuan-Sheng Chen, Wei-Ming Lee, Wei-Yau Shia
The Thai Journal of Veterinary Medicine
A ten-year-old, 16.7 kg mixed-breed dog presented with chronic lameness and delayed healing of discharging wounds in the right pelvic limb following a puncture injury for approximately 6 months. Debridement surgery had been performed, but the tissue infection was persistent. Plain radiographic examination showed neither increased soft tissue opacity nor foreign body (FB) existence near the discharging wound of the right pelvic limb. After the second debridement surgery, closed drainage tubes were placed, and susceptible antibiotic therapy was prescribed in an attempt to control the persistent infection, but the fluid draining from the wound was still purulent. Therefore, fistulography was …
Computed Tomographic Appearance Of Bladder And Vaginal Leiomyoma In Dog: Case Report,
2021
Chulalongkorn University
Computed Tomographic Appearance Of Bladder And Vaginal Leiomyoma In Dog: Case Report, Sirirat Phantharangsi, Sukanya Maneein, Sekkarin Ploypetch
The Thai Journal of Veterinary Medicine
An 18-year-old, female Shih Tzu dog was presented at Prasu-Arthorn Animal Hospital, Faculty of Veterinary Science, Mahidol University with the clinical signs of abdominal enlargement, constipation and urine incontinence. The survey abdominal radiographs demonstrated only a caudal large oval soft tissue abdominal mass and abdominal ultrasonography was suggested to indicate the mass origin. However, ultrasonographic finding did not identify the mass location and tissue invasiveness so computed tomography (CT) was requested. The CT result revealed that the mass was a mixed heterogenous attenuating and fluid attenuating mass (8.56 x 5.47 x 6.72 cm) at the uterine body and involved the …
Deterring Non-Target Birds From Toxic Bait Sites For Wild Pigs,
2021
USDA APHIS Wildlife Services NWRC
Deterring Non-Target Birds From Toxic Bait Sites For Wild Pigs, Nathan P. Snow, Joseph M. Halseth, Justin A. Foster, Michael J. Lavelle, Justin W. Fischer, Michael P. Glow, Ingrid A. Messer, Seth M. Cook, Kurt C. Vercauteren
United States Department of Agriculture Wildlife Services: Staff Publications
Toxic baiting of wild pigs (Sus scrofa) is a potential new tool for population control and damage reduction in the US. Field trials testing a prototype toxic bait (HOGGONE 2 containing 5% sodium nitrite [SN]), though, revealed that wild pigs spilled small particles of toxic bait outside of bait stations which subsequently created hazards for non-target species that consumed those particles, primarily passerine birds. To deter non-target birds from consuming particles of spilled bait, we tested four deterrents at mock bait sites (i.e., baited with bird seed) in north-central Colorado, USA during April–May 2020. We found a programable, inflatable deterrent …
Towards A More Comprehensive Understanding Of Wild Pig (Sus Scrofa) Impacts On Agricultural Producers: Insights From A Texas Case Study,
2021
Warner College of Natural Resources
Towards A More Comprehensive Understanding Of Wild Pig (Sus Scrofa) Impacts On Agricultural Producers: Insights From A Texas Case Study, Keith M. Carlisle, Nicole Didero, Sophie Mckee, Julie Elser, Stephanie A. Shwiff
United States Department of Agriculture Wildlife Services: Staff Publications
This research investigates the impacts of invasive wild pigs (Sus scrofa Linneaus) on agricultural producers in Texas, with the aim of identifying and describing all categories of wild pig impacts and quantifying the extent of producers’ over- or underestimation of their total wild pig-related costs in 2018, as compared to calculations based upon data subsequently provided by the producers about individual wild pig-related costs and losses. Based on interviews with 23 producers in 16 Texas counties, we identified more than 20 discrete categories of negative impacts and negligible positive impacts associated with wild pigs. Among them were categories that have …
Landscape Transformations Produce Favorable Roosting Conditions For Turkey Vultures And Black Vultures,
2021
State University of New York College of Environmental Science and Forestry
Landscape Transformations Produce Favorable Roosting Conditions For Turkey Vultures And Black Vultures, Jacob E. Hill, Kenneth F. Kellner, Bryan M. Kluever, Michael L. Avery, John S. Humphrey, Eric A. Tillman, Travis L. Devault, Jerrold L. Belant
United States Department of Agriculture Wildlife Services: Staff Publications
Recent increases in turkey vulture (Cathartes aura) and black vulture (Coragyps atratus) populations in North America have been attributed in part to their success adapting to human-modified landscapes. However, the capacity for such landscapes to generate favorable roosting conditions for these species has not been thoroughly investigated. We assessed the role of anthropogenic and natural landscape elements on roosting habitat selection of 11 black and 7 turkey vultures in coastal South Carolina, USA using a GPS satellite transmitter dataset derived from previous research. Our dataset spanned 2006–2012 and contained data from 7916 nights of roosting. Landscape …
Reproductive Success Of Captive-Reared Allegheny Woodrats (Neotoma Magister) Released Into Genetically Depauperate Populations,
2021
Towson University
Reproductive Success Of Captive-Reared Allegheny Woodrats (Neotoma Magister) Released Into Genetically Depauperate Populations, Miracle M. Davis, Timothy J. Smyser, Scott A. Johnson, Joseph Duchamp, Jeffery L. Larkin, Robert K. Swihart, Jacqueline M. Doyle
United States Department of Agriculture Wildlife Services: Staff Publications
Habitat fragmentation and degradation have led to a proliferation of small and isolated populations that are vulnerable to genetic erosion. Reduction in habitat and concomitant declines in population connectivity can expediate the collapse of species that exist as natural metapopulations. In recent years, Allegheny woodrats (Neotoma magister) have experienced local extirpations and declines in genetic diversity across their range due to disease-related mortality, reduced food availability, and disruption of connectivity among subpopulations. In response, we developed a captive-breeding program to facilitate genetic management of isolated woodrat populations in Indiana. Between 2010 and 2012, 27 captive-reared individuals were released to four …
