Veterinary Medicine Commons^™

Comparative Evaluation Of Clinical Samples From Naturally Infected Dogs For Early Detection Of Canine Distemper Virus, Muhammad Zubair Shabbir, Masood Rabbani, Arfan Ahmad, Ali̇ Ahmed, Khushi Muhammad, Ikram Anwar

Turkish Journal of Veterinary & Animal Sciences

Early detection of canine distemper (CD) is critical for appropriate and timely treatment. The present study included 45 suspected cases with respiratory signs for a comparative evaluation of clinical samples in the early detection of canine distemper virus (CDV). Thirty-four dogs exhibited digestive system involvement, but none showed any neurological symptoms. Clinical progression of CD, based on age, sex, vaccination status, disease, and clinical findings, was observed. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect viral nucleoprotein RNA in plasma, conjunctival and nasal swabs. Only 10 dogs were positive for CDV infection. Among the 45 dogs, CDV RNA …

Gene Delivery In The Equine Cornea: A Novel Therapeutic Strategy, Dylan G. Buss, Ajay Sharma, Elizabeth A. Giuliano, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To determine if hybrid adeno-associated virus serotype 2/5 (AAV5) vector can effectively deliver foreign genes into the equine cornea without causing adverse side effects. The aims of this study were to: (i) evaluate efficacy of AAV5 to deliver therapeutic genes into equine corneal fibroblasts (ECFs) using enhanced green fluorescent protein (EGFP) marker gene and (ii) establish the safety of AAV5 vector for equine corneal gene therapy.

Animal Material—Primary ECF cultures were harvested from healthy donor equine corneas. Cultures were maintained at 370C in humidified atmosphere with 5% CO2.

Procedure—AAV5 vector expressing EGFP under control of hybrid cytomegalovirus (CMV) + chicken …

Collection And Preparation Of Milk Samples For Microbiological Culturing, Jeffrey M. Bewley, Robert J. Harmon, Stephan Locke

Agriculture and Natural Resources Publications

In developing individual farm mastitis control and treatment strategies, it is often necessary to characterize the types of bacteria that are present on your farm. To answer this question, a microbiological analysis, or milk culture, must be performed on milk samples collected from cows showing clinical or subclinical signs of mastitis. Results of the milk cultures will help identify which bacteria are causing the mastitis. In turn, this information can be used to alter mastitis control, prevention, and treatment options to fit your herd’s conditions.

During an investigation of a herd dealing with high somatic cell counts or a high …

Isolation And Cultivation Of Equine Corneal Keratocytes, Fibroblasts And Myofibroblasts, Dylan G. Buss, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To establish an in vitro model for the investigation of equine corneal wound healing. To accomplish this goal, a protocol to isolate and culture equine corneal keratocytes, fibroblasts and myofibroblasts was developed.

Animal material—Equine corneal buttons were aseptically harvested from healthy research horses undergoing humane euthanasia for reasons unrelated to this study. Slit-lamp biomicroscopy was performed prior to euthanasia by a board-certified veterinary ophthalmologist to ensure that all samples were harvested from horses free of anterior segment disease.

Procedure—Equine corneal stroma was isolated using mechanical techniques and stromal subsections were then cultured. Customized media at different culture conditions was used …

Oxidative Stress In Shaal Sheep Of Different Age Groups, Jamileh Salar-Amoli, Ali Baghbanzadeh

Turkish Journal of Veterinary & Animal Sciences

Oxidative stress plays an important role in the etiology and/or progression of a number of diseases and aging. In this cross-sectional study, some oxidative stress biomarkers (malondialdehyde [MDA] as a lipid peroxidation biomarker, ferric reducing/antioxidant power [FRAP], and total nonprotein SH groups) in plasma samples of Shaal ewes of different age groups were assessed. For all 3 measured parameters, the youngest age group (10-30 months old) had the lowest amounts. Although FRAP levels increased slightly and age dependently, nonprotein SH group content showed a decreasing trend after a peak in the 31-50 months group. The highest MDA concentration was observed …

Ospc In The Pathogenesis Of Borrelia Burgdorferi, Sunita V. Seemanapalli

LSU Doctoral Dissertations

Lyme disease is a multisystem disorder caused by the tick vector Borrelia burgdorferi. During its life cycle between the tick vector and the mammalian host, Borrelia up- and down-regulates the expression of its surface lipoproteins. Of its many surface lipoproteins, the Outer surface protein C (OspC) is crucial for initial mammalian infection. OspC has a common role shared with other lipoproteins of protection against host innate defences and a unique function in facilitating the dissemination of B. burgdorferi in the murine host. The structure of OspC was solved in 2001 and the lipoprotein was found to be predominantly alpha-helical with …

Development Of A New Tacaribearenavirus Infection Model And Its Use To Explore Antiviral Activity Of A Novelaristeromycin Analog, Brian B. Gowen, M. H. Wong, D. Larson, W. Ye, K. H. Jung, E. J. Sefing, Ramona T. Skirpstunas, Donald F. Smee, John D. Morrey, S. W. Schneller

Animal, Dairy, and Veterinary Science Faculty Publications

Background

A growing number of arenaviruses can cause a devastating viral hemorrhagic fever (VHF) syndrome. They pose a public health threat as emerging viruses and because of their potential use as bioterror agents. All of the highly pathogenic New World arenaviruses (NWA) phylogenetically segregate into clade B and require maximum biosafety containment facilities for their study. Tacaribe virus (TCRV) is a nonpathogenic member of clade B that is closely related to the VHF arenaviruses at the amino acid level. Despite this relatedness, TCRV lacks the ability to antagonize the host interferon (IFN) response, which likely contributes to its inability to …

Alterations In Serum Tartrate-Resistant Acid Phosphatase And C-Terminal Telopeptide Of Type I Collagen In Experimental Canine Osteotomies Fixed Using 2 Different Techniques, Michail Paskalev, Svetozar Krastev

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to monitor serum tartrate-resistant acid phosphatase (TRAP) and C-terminal telopeptide fragments of type I collagen (CTX) over time in canine experimental osteotomies fixed using 2 osteosynthesis techniques, to determine the relevance of both markers in monitoring bone healing, and to investigate the influence of the 2 osteosynthesis techniques on their concentrations. Transperiosteal osteotomy of the diaphyses of the right tibia and fibula was performed in 12 dogs. The dogs were then randomly assigned to 1 of 2 groups: group 1 (6 dogs) underwent osteotomy fixed with intramedullary osteosynthesis (IMO) and group 2 (6 …

Anatomical Study Of The Brain Of The African Ostrich, Ke-Mei Peng, Yueping Feng, Gaoying Zhang, Huazhen Liu, Hui Song

Turkish Journal of Veterinary & Animal Sciences

The anatomical characteristics of the African ostrich brain were investigated in this study. The average weight, length, and width of the total brain are 26.34 g, 59.26 mm, and 42.30 mm, respectively. The cerebellum appears relatively well developed and obviously protrudes dorsally. The posterior superior part of the cerebellar vermis almost forms an angle of 130°. The ostrich brain has many more transverse fissures of the cerebellar vermis than do the brains of domestic fowls. Therefore, the surface area of the African ostrich's cerebellum is larger. The formation of the cerebrum is an obtuse triangle. Its surface is smooth, without …

The Effect Of Feeding Frequency On The Hormonal Profile, Carcass Characteristics, And Feedlot Performance In Iranian Holstein Calves, Armin Towhidi, Aboulfazl Zali, Abbas Khoshsokhan, Homayoon Khazali, Mehdi Zhandi, Kamran Rezayazdi, Reza Masoumi, Hamid Gholami

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to investigate the effect of feeding frequency on body weight, carcass characteristics, and hormonal profile in Iranian Holstein calves. The study included 12 Holstein bull calves that were assigned to 2 groups according to initial live body weight. The animals were fed a TMR ration based on live body weight. The calves were fed a standard diet for 31 weeks; control calves were fed twice a day and treatment calves were fed 7 times a day. Live body weights were recorded every 21 days. Blood samples were collected 1 h after the final …

A Comparitive Study On Traumatic Reticuloperitonitis And Traumatic Pericarditis In Egyptian Cattle, Mohamed M. Ghanem

Turkish Journal of Veterinary & Animal Sciences

This study was carried out on 15 Holstein cows divided into 3 groups of 5. Group 1 was the control, group 2 had traumatic reticuloperitonitis (TRP), and group 3 had traumatic pericarditis (TP). Comparison between the groups included clinical, hematological, biochemical, and ultrasonographic changes. TRP cows had arched backs, a sharp decrease in milk yield, and a reluctance to move, with abduction of the fore limbs. TP cows had edema of the brisket and submandibular region, with jugular vein distension and pulsation. TRP cows had a significant increase in PCV, leukocytes, and neutrophils, and a significant decrease in RBC, hemoglobin, …

Identification Of Babesia And Theileria Species In Small Ruminants In Central Anatolia (Turkey) Via Reverse Line Blotting, Abdullah İnci̇, Anil İça, Alparslan Yildirim, Önder Düzlü

Turkish Journal of Veterinary & Animal Sciences

This study was conducted between January 2006 and September 2008 to identify Babesia and Theileria species in sheep and goats in Central Anatolia via reverse line blotting (RLB). In total, 421 sheep and 152 goats were randomly selected from herds located in Kayseri, Yozgat, and Sivas provinces (all in central Turkey). The amplified hypervariable V4 region of the 18S rRNA gene of ovine piroplasm species, including Theileria lestoquardi, T. ovis, Babesia ovis, B. motasi, and B. crassa, was hybridized against species-specific probes. Theileria ovis and B. ovis were observed in the study animals. Among the 573 small ruminants included in …

The Effect Of A Single Administration Of Parenteral Oxytetracycline And Flunixin Meglumine Combination On The Reproductive Performance Of Dairy Cows With Subclinical Endometritis, Çağatay Tek, Ahmet Sabuncu, Serkan İki̇z, Funda Bağcigi̇l, Mehmet Can Gündüz, M. Ragip Kiliçarslan, Yakut Özgür

Turkish Journal of Veterinary & Animal Sciences

This study explored the effects of a single administration of flunixin meglumine (acting as a non-steroidal anti-inflammatory drug) and oxytetracycline combination on the reproductive performance of cows diagnosed with subclinical endometritis during the postpartum period. Cows aged 3 to 9 at 40 to 60 days postpartum were studied. Endometrial samples were collected from cows that had a uterine cornu (middle of the cornu) width of greater than 3.5 cm in the ultrasonographic examination, and 40 cows diagnosed with subclinical endometritis were included in the study. Cows in group 1 (n = 20) received a single dose of 20 mg of …

Evaluation Of The Efficacy Of Esterified Glucomannan, Sodium Bentonite, And Humic Acid To Ameliorate The Toxic Effects Of Aflatoxin In Broilers, Hasan Ghahri, Reza Habibian, Mehdi Abdollah Fam

Turkish Journal of Veterinary & Animal Sciences

A study was conducted to determine the efficacy of esterified glucomannan, sodium bentonite, and humic acid in counteracting the toxic effects of aflatoxin in a naturally contaminated diet fed to broilers. Seven-day-old chicks were randomly assigned to 1 of the 9 dietary treatments. The treatments were as follows: 1) control; 2) naturally contaminated diet with aflatoxin; 3, 4, 5, 6, and 7) naturally contaminated diet with aflatoxin supplemented with 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% humic acid, respectively; 8 and 9) naturally contaminated diet supplemented with 0.5% sodium bentonite and 0.1% esterified glucomannan, respectively. Compared with the control, the naturally …

Evaluation Of Different Laparoscopic Sterilization Techniques In A Canine Birth Control Program, Avijit Dutta, Swapan Maiti, Ajith Parameswaran Pillai, Naveen Kumar

Turkish Journal of Veterinary & Animal Sciences

Three laparoscopic sterilization techniques under xylazine-ketamine anesthesia were evaluated at a 10 mmHg pressure gradient of CO_2 in 60 mongrel bitches, equally divided into 3 groups (A, B, and C). Laparoscopic bilateral oopherectomy was done in group A, laparoscopic ovariohysterectomy by endoclip and electrocautery in group B, and sterilization by electrocautery in group C. All of the animals recovered from anesthesia smoothly after the completion of laparoscopic surgery. Intraoperative observations revealed good visualization of the reproductive organs at 10 mmHg of pressure in the Trendelenburg position. Comparison among the various laparoscopic techniques revealed a more effective hemostasis with titanium endoclip …

Preliminary Findings Of Behavioral Patterns In Captive Alpine Musk Deer (Moschus Sifanicus) And Prospects For Future Conservation, Xiuxiang Meng, Hong Yang, Qisen Yang, Zuojian Feng, Xu Peng, Genevieve C. Perkins

Turkish Journal of Veterinary & Animal Sciences

Captive farming of alpine musk deer (Moschus sifanicus) in China has been used for conservation and harvesting of musk since the mid 1950s. Despite this long history, management practices and captive breeding have been primarily based on trial and error due to lack of behavioral and ecological information about this vulnerable species. Understanding behavioral patterns plays a vital part in determining appropriate management systems; hence the aim of this study was to determine the effect of captivity on behavioral patterns of alpine musk deer by comparing wild-caught and the captive-born alpine musk deer. From August 2002 to January 2003, the …

Effect Of Urea And Oregano Oil Supplementation On Growth Performance And Carcass Characteristics Of Lamb Fed Diets Containing Different Amounts Of Energy And Protein, Önder Canbolat, Ali̇ Karabulut

Turkish Journal of Veterinary & Animal Sciences

The experiment was carried out using a 4 × 4 factorial design. There were 16 experimental groups. Each group consisted of 3 lambs. The experiment lasted 56 days. During the experiment, the oregano oil (0 and 5 g/kg DM) and urea (0, 6, 12, and 18 g/head per day) were given to lambs consuming low energy/low protein diets (2350 Kcal/kg DM/ 10.80% CP/DM, respectively) and normal energy/normal protein (2600 Kcal/kg DM, 14.97% CP/DM, respectively) diets. Daily body weight gain of lambs consuming normal energy/normal protein diets and supplemented with 6, 12 and 18 g/head per day urea were significantly (P …

First Reported Case Of Chironomid Midge Larva Infestations In Rainbow Trout (Oncorhynchus Mykiss Walbaum 1792) Eggs, Şevki̇ Kayiş, İlhan Altinok, Fi̇kri̇ Balta

Turkish Journal of Veterinary & Animal Sciences

Six days after transfer of eyed stage rainbow trout (Oncorhynchus mykiss) eggs into an incubator, egg mortality occurred during the winter of 2007. During the outbreak, approximately 30% of the eggs died. Rheocricotopus fuscipes (Kieffer, 1909) and Eukiefferiella claripennis (Lundbeck, 1898) (subfamily Orthocladiinae), Chironomid midge larvae were observed on the eggs by examining the eggs under the microscope. Histological sections of the infested eggs exhibited midge bite marks. This is the first report of Chironomid midges preying on fish eggs.

Blood Plasma Mineral Profile And Health Status In Postpartum Cows Fed An Anionic Diet Before Parturition, Cazim Crnkic, Salko Muratovic, Slavica Piplica, Aida Kavazovic, Stanimir Kutlaca

Turkish Journal of Veterinary & Animal Sciences

The effects of an anionic versus cationic (control) diet on plasma mineral levels and health status were investigated in 24 dry pregnant Holstein-Friesian cows during the last 3 weeks of pregnancy. The cows were in their third or fourth parity, had a moderate level of milk production in their previous lactation, and did not have a history of milk fever. The anionic diet significantly improved plasma calcium status, as compared to the control (2.18 vs. 1.99 mmol L^{-1}) and nonsignificantly reduced the incidence of subclinical hypocalcemia (1/12 vs. 5/12) without affecting other periparturient diseases. Other plasma mineral levels were unaffected, …

Expression And Tissue Distribution Of Extracellular Calcium-Sensing Receptor (Casr) Mrna In Chickens, Guangcun Deng, Jianmin Bi, Jian Qiao, Guiei He, Boning Wu, Miaojie Zhang, Nana Lv

Turkish Journal of Veterinary & Animal Sciences

Extracellular calcium-sensing receptor (CaSR) is a recently discovered G-protein-coupled receptor that may play a key role in regulating parathyroid hormone secretion and calcium homeostasis. In the present study, based on the reported sequence of the chicken CaSR gene (GenBank accession: XM_416491), specific primers were designed and synthesized, and CaSR gene mRNA expression and tissue distribution in chickens were analyzed using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) analysis. It was observed that CaSR mRNA was expressed in the kidney and parathyroid to a greater degree than in the small intestine, oviduct, and brain (P < 0.01). Sequence analysis of the PCR products obtained from the 5 tissues showed that these cDNAs shared 100% homology with each other and the corresponding region of the reported CaSR cDNA. The results may provide a basis and additional insight for understanding the physiological function of CaSR in chickens.