Effect Of A Direct-Fed Microbial (Eubios 1090) In The Presence Of Antibiotics (Carbadox Or Ctc-Denagard) On Post-Weaning Pig Growth Performance And Immune Response,
2010
University of Arkansas, Fayetteville
Effect Of A Direct-Fed Microbial (Eubios 1090) In The Presence Of Antibiotics (Carbadox Or Ctc-Denagard) On Post-Weaning Pig Growth Performance And Immune Response, Kimberley Santos, Charles Maxwell, Elizabeth Kegley, Charles Rosenkrans
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
A study was conducted to determine the effects of a probiotic (Eubios 1090), in the presence of two different antibiotics, on performance in nursery pigs. A total of 216 pigs were weaned at an average of 21 d, blocked by initial body weight (BW = 6.79 kg), and distributed into 32 pens of 6 to 7 pigs per pen in an offsite nursery facility. Pens were randomly assigned to 1 of 4 dietary treatments (8 pens per treatment) that were fed throughout post-weaning phase 1 (day (D) 0 to 10), phase 2 (D 10 to 20), and phase 3 (D …
Applicability And Integration Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Bone Screws In Equine Bone,
2010
Louisiana State University and Agricultural and Mechanical College
Applicability And Integration Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Bone Screws In Equine Bone, Timm Gudehus
LSU Master's Theses
To compare insertion temperatures and torques of Hydroxyapatite (HA) coated and uncoated 5.5 mm AO cortical screws in equine third metatarsal bones (MTIII) in vitro, and to compare insertion and extraction torques of HA coated and uncoated screws after 4, 8, 12 and 16 weeks of healing in equine third metacarpal bones (MCIII) in vivo. No significant temperature differences were recorded in cadaveric bones for AO and HA coated screws. Insertion torques were significantly higher for HA coated implants compared to uncoated screws. In vivo, the AO screws lost 50% of their initial stability within 4 weeks of healing and …
Carprofen-Induced Oxidative Stress In Mitochondria Of The Colonic Mucosa Of The Dog,
2010
Louisiana State University and Agricultural and Mechanical College
Carprofen-Induced Oxidative Stress In Mitochondria Of The Colonic Mucosa Of The Dog, Lynne A. Snow
LSU Master's Theses
Objectives 1) To measure conductance and permeability of canine colonic mucosa exposed to increasing concentrations of carprofen. 2) To compare conductance and permeability of canine colonic mucosa exposed to carprofen or 2,4-dinitrophenol (DNP) and tempol blockade. Design In vitro randomized block design Animal 20 mixed breed dogs Methods Conductance, mannitol flux, and histology were evaluated in colonic mucosa mounted in Ussing chambers. Mucosa was first exposed to increasing concentrations of carprofen. Mucosa was then exposed to either carprofen (200 μg/ml) or DNP (0.25mM) +/- tempol (1mM) pretreatment. Conductance over time, mannitol fluxes, and frequency of histologic categories were analyzed for …
The Role Of Bone Marrow In Siv Pathogenesis Using The Rhesus Macaque Model,
2010
Louisiana State University and Agricultural and Mechanical College
The Role Of Bone Marrow In Siv Pathogenesis Using The Rhesus Macaque Model, Amy F. Gill
LSU Doctoral Dissertations
CD4+ memory T cells are depleted in mucosal tissues post human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) infection without restoration to pre-infection levels during progressive course of disease. Bone marrow (BM) as a hematopoietic organ has been investigated for hematologic and morphologic changes during HIV infection. However, BM as a primary lymphoid tissue during HIV infection has been poorly characterized. We proposed BM was also a site of CD4+ T cell depletion driven by increased apoptosis during progressive HIV disease. We chose to investigate bone marrow changes using the premier non-human primate Rhesus macaque SIV model for the …
Role Of Gastrointestinal Multidrug Resistance (Mdr1) Gene And P-Glycoprotein (P-Gp) In The Oral Absorption Of Methadone In Horses,
2010
Louisiana State University and Agricultural and Mechanical College
Role Of Gastrointestinal Multidrug Resistance (Mdr1) Gene And P-Glycoprotein (P-Gp) In The Oral Absorption Of Methadone In Horses, Renata Lehn Linardi
LSU Doctoral Dissertations
Methadone is a mu-opioid receptor agonist which is a very effective analgesic used to treat moderate to severe acute and chronic pain in humans. Due to methadone’s minimal undesirable side-effects in people, we believed it could be of use in horses as an analgesic agent. As found with the majority of lipophilic drugs, absorption of methadone occurs primarily in the small intestine via transcellular transport and its absorption is regulated by P-glycoprotein. P-glycoprotein is a transmembrane transporter protein encoded by the multidrug resistance gene, which is constitutively expressed in the apical membrane of enterocytes of various species. This protein may …
In Vivo And In Vitro Pathogenesis Of Francisella Asiatica In Tilapia Nilotica (Oreochromis Niloticus),
2010
Louisiana State University and Agricultural and Mechanical College
In Vivo And In Vitro Pathogenesis Of Francisella Asiatica In Tilapia Nilotica (Oreochromis Niloticus), Esteban Soto
LSU Doctoral Dissertations
Francisella asiatica is a Gram negative, facultative intracellular pathogen that causes fish francisellosis. In this project, homologues to the F. tularensis iglABCD genes were found present in F. asiatica. As few as 23 F. asiatica bacteria injected in the peritoneum were found capable of causing mortalities in tilapia nilotica (Oreochromis niloticus), and even fewer were enough to cause pathological changes. We examined the in vivo and in vitro interaction of F. asiatica wild type (WT) and a ÄiglC strain with tilapia and tilapia head kidney derived macrophages (HKDM). The ÄiglC was found to be attenuated following intraperitoneal and immersion challenges …
In Vitro And In Vivo Evaluation Of A Brucella Putative Hemagglutinin,
2010
Louisiana State University and Agricultural and Mechanical College
In Vitro And In Vivo Evaluation Of A Brucella Putative Hemagglutinin, Lauren E. Duhon
LSU Doctoral Dissertations
Brucellosis, a zoonotic disease caused by Brucella spp., presents both health and economic difficulties for livestock, wildlife, and humans. While brucellosis is nearly eradicated in the United States, the disease remains detrimental in many countries worldwide. Attempts to produce a safe and effective small ruminant vaccine have been met with limited success. The current vaccine for bovine brucellosis in the United States is B. abortus RB51. This strain transiently colonizes the host and induces a cell-mediated immune response. Levels of protection have not been demonstrated in goats and thus it is considered a relatively poor caprine vaccine that probably does …
Ospc In The Pathogenesis Of Borrelia Burgdorferi,
2010
Louisiana State University and Agricultural and Mechanical College
Ospc In The Pathogenesis Of Borrelia Burgdorferi, Sunita V. Seemanapalli
LSU Doctoral Dissertations
Lyme disease is a multisystem disorder caused by the tick vector Borrelia burgdorferi. During its life cycle between the tick vector and the mammalian host, Borrelia up- and down-regulates the expression of its surface lipoproteins. Of its many surface lipoproteins, the Outer surface protein C (OspC) is crucial for initial mammalian infection. OspC has a common role shared with other lipoproteins of protection against host innate defences and a unique function in facilitating the dissemination of B. burgdorferi in the murine host. The structure of OspC was solved in 2001 and the lipoprotein was found to be predominantly alpha-helical with …
Pathological And Clinical Findings And Tissue Distribution Of Salmonella Gallinarum Infection In Turkey Poults,
2010
TÜBİTAK
Pathological And Clinical Findings And Tissue Distribution Of Salmonella Gallinarum Infection In Turkey Poults, Lati̇fe Beyaz, Ayhan Atasever, Fuat Aydin, K. Semi̇h Gümüşsoy, Seçi̇l Abay
Turkish Journal of Veterinary & Animal Sciences
This study aimed to experimentally induce Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Gallinarum) infection in turkey poults in order to detect S. Gallinarum using immunohistochemical, bacteriological, and histological procedures. The study included 90 white turkey poults that were divided into 3 groups (1-day-old, 3-week-old, and 2-month-old), each of which was orally inoculated with an inoculum of 0.3 mL of S. Gallinarum strain 9 broth culture that contained approximately 2 × 10^4, 1 × 10^6, and 1 × 10^9 cfu/mL, respectively. The duration of the experiment was 18 days. Clinical, pathological, and bacteriological findings were evaluated daily until 18 days …
Contemporary Grouping In Mixed-Size Dairy Herds Experiencing Four Seasons,
2010
TÜBİTAK
Contemporary Grouping In Mixed-Size Dairy Herds Experiencing Four Seasons, Mohammad Ali̇ Nilforooshan
Turkish Journal of Veterinary & Animal Sciences
Countries with different herd structures and climates use different combinations of herd, year, and season to define the effect of their climatic conditions and management. Estimates of variance components and Akaike information criterion values resulting from 8 analytical models differentiated by different combinations of herd, year and season effects were compared for milk and fat yields. Data consisted of pedigree and records of 11,850 Holsteins freshening in 38 dairy herds of Isfahan, Iran, between January 1994 and October 2001. Heritability estimates were lowest for the models that used herd-year combination as a random effect. Moreover, the use of these models …
Immune Response To Type-2 Porcine Circovirus In Weaned Pigs,
2010
TÜBİTAK
Immune Response To Type-2 Porcine Circovirus In Weaned Pigs, Hong Chen, Ke-Ying Zhang, Xue-Mei Ding, Zhi-Wen Xu, Dai-Wen Chen, Gang Tian
Turkish Journal of Veterinary & Animal Sciences
Sixteen crossbred barrows (Duroc × Landrace × Yorkshire) weaned at 28 days with an initial body weight of 5.12-8.64 kg and no antibodies to PCV2 were used in a 5-week trial to evaluate the immune response after intranasal inoculation with type-2 porcine circovirus. The percentage of peripheral blood ANAE+ T cells, PCV2 antibody titer, immunoglobulins, and serum IFN-\gamma, IL-2, and sIL-2R levels were determined. The results show that the percentage of peripheral blood ANAE+ T cells was notably higher in inoculated pigs 5 days post-inoculation (dpi) and significantly lower 21 dpi than in the control group. The anti-PCV2 titer in …
The Performance Of A Small-Scale, High-Density, Continuous System For Culturing The Rotifer Brachionus Plicatilis,
2010
TÜBİTAK
The Performance Of A Small-Scale, High-Density, Continuous System For Culturing The Rotifer Brachionus Plicatilis, Umur Önal, İhsan Çeli̇k, Sebahatti̇n Ergün
Turkish Journal of Veterinary & Animal Sciences
The performance of a high-density continuous system was evaluated as an alternative to conventional low-density batch-culture systems for culturing the rotifer Brachionus plicatilis. Rotifer densities ranging from 1700 to 3760 individuals/mL were achieved in 4 different trials that lasted 10-18 days. Problems associated with NH_3-N and pH were controlled by increasing the water exchange rate and periodic addition of ammonia remover. While NH_3-N and pH values were 0-8.8 mg/L and 6.9-9.3, respectively, the most important problem was the accumulation of TSS at concentrations of up to 85 mL/L within a very short time after start-up. Further studies are required to …
Reference Values For Some Haematological, Haematochemical, And Electrophoretic Parameters In The Girgentana Goat,
2010
TÜBİTAK
Reference Values For Some Haematological, Haematochemical, And Electrophoretic Parameters In The Girgentana Goat, Giuseppe Piccione, Stefania Casella, Lucio Lutri, Irene Vazzana, Vincenzo Ferrantelli, Giovanni Caola
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to determine haematological, haematochemical, and electrophoretic reference values for the Girgentana goat in order to form a basis for clinical interpretation. The study included 348 female Girgentana goats aged from 1 to 6 years. The animals were divided into 3 groups: goats aged 1-2 years (group A), goats aged 3-4 years (group B), and goats aged 5-6 years (group C). Blood samples were collected from each animal every morning at the same time (0800) by means of jugular venipuncture, and red blood cell (RBC), white blood cell (WBC), neutrophil (NEU), lymphocyte (LYM), monocyte (MONO), …
A Common Tortoise Tick, Hyalomma Aegyptium Linne 1758 (Acari: Ixodidae), Identified On Eastern Hedgehog (Erinaceus Concolor Martin 1838) In Central Anatolia, Serkal Gazyağci, Nursel Aşan, Yasi̇n Demi̇rbaş
Turkish Journal of Veterinary & Animal Sciences
Ticks are obligate blood sucker arthropods that infect animals and humans. A common tortoise tick, Hyalomma aegyptium, was collected from a young and an adult male hedgehog, Erinaceus concolor, from Central Anatolia in July 2008. More ticks were determined on the young one. This is the second record of tortoise tick that parasitizes a hedgehog.
Conception Rates For Standing Estrus And Fixed-Time Insemination In Dairy Heifers Synchronized With Gnrh And Pgf_{2\Alpha},
2010
TÜBİTAK
Conception Rates For Standing Estrus And Fixed-Time Insemination In Dairy Heifers Synchronized With Gnrh And Pgf_{2\Alpha}, Samir Z. El-Zarkouny
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to evaluate the effectiveness of GnRH PGF-based protocols on reproductive outcome in Holstein heifers. Heifers were randomly assigned to the control group (n = 14), or treatment groups (n = 50) treated with GnRH (day -7) and PGF_{2\alpha} (day 0). Heifers that exhibited estrus were inseminated (Select Synch, n = 34) and heifers that did not exhibit estrus up to 48 h after PGF_{2\alpha} injection received a second GnRH injection and were fixed-time inseminated (Ovsynch, n = 16). Conception rates were 40%, 55.9%, and 31.3%, and overall pregnancy rates (P < 0.05) were 64.3%, 97%, and 87.5% in the control, Select Synch, and Ovsynch groups, respectively. Ovsynch heifers had 1.8 ± 0.5 and 3.2 ± 0.6 ng mL^{–1} of serum P4, versus 3.3 ± 0.5 and 3.8 ± 0.6 ng mL–1 in the Select Synch heifers at the time of the first GnRH and PGF_{2\alpha} injection, respectively. Conception rates were 55.6% and 58.3% when P4 concentrations were high, and were 50% and 25% when P4 concentrations were low at the time of the first GnRH and PGF_{2\alpha} injection, respectively. Dairy heifers synchronized with GnRH and PGF_{2\alpha} should be inseminated when estrus is observed, and heifers that do not exhibit estrus up to 48 h after PGF_{2\alpha} injection should be given a second GnRH injection and fixed-time inseminated 16-20 h later.
Effect Of Pre-Evisceration, Skin-On Carcass Decontamination Sanitation Strategies For Reducing Bacterial Contamination Of Cattle During Skinning, Mehmet Çalicioğlu, Dennis R. Buege, John B. Luchansky
Turkish Journal of Veterinary & Animal Sciences
The effectiveness of pre-evisceration, skin-on carcass sanitation on reducing bacterial contamination of beef carcasses was tested using 3 cattle per treatment and 3 cattle as controls at each of 3 abattoirs in southern Wisconsin. The sanitation procedure included stunning, bleeding, tying off the esophagus, sealing the anus, and then sanitizing the hide with: i) 20% trisodium phosphate, ii) 200 ppm iodophor, iii) 75% ethanol, or iv) hot water (ca. 80 °C). Two sets of combined sponge samples (3 × 100 cm^2) were taken from the hide before and after the sanitation step, as well as from the carcass after the …
All-Trans Retinoic Acid Regulates Intracellular Redox Status In Marc-145 Cells Infected With Porcine Reproductive And Respiratory Syndrome Virus, Yan Lin, De Wu
Turkish Journal of Veterinary & Animal Sciences
A relationship between oxidative stress, and apoptosis and/or death has been suggested for many years. The objective of this study was to determine the influence of all-trans retinoic acid (ATRA) on the cell survival rate and the antioxidant defense system. MARC-145 cells were treated with ATRA after infection with porcine reproductive and respiratory syndrome virus (PRRSV). The cell survival rate, total antioxidant capability (T-AOC), glutathione peroxidase (GSH-P_X) activity, superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and glutathione (GSH) content were determined in samples collected from cells incubated with ATRA for 24 or 48 h. The results show that PRRSV …
Maedi-Visna Virus In Turkish Sheep: A Preliminary Serological Survey Using Elisa Tests,
2010
TÜBİTAK
Maedi-Visna Virus In Turkish Sheep: A Preliminary Serological Survey Using Elisa Tests, Silvia Preziuso, Mehmet Erman Or, Monica Giammarioli, Abdullah Kayar, Francesco Feliziani, Remzi̇ Gönül, Silvana Farneti, Cağla Parkan Yaramiş, Carlo Valente, Vincenzo Cuteri
Turkish Journal of Veterinary & Animal Sciences
The maedi-visna virus (MVV) is distributed worldwide and, to date, no epidemiological surveys have been carried out using ELISA testing in Turkey. The aims of this study were as follows: i) to verify the diagnostic performance of a home-made ELISA test (HM-ELISA) using Turkish ovine sera in comparison with other commercially available ELISA tests, and ii) to perform a preliminary epidemiological survey in İstanbul province. The MVV seroprevalence in İstanbul province was 15.3%. Older sheep were 5 times more likely to be positive than younger animals, ewes were 3 times more likely to be positive than rams, and all the …
Nasal Squamous Cell Carcinoma In A Cow,
2010
TÜBİTAK
Nasal Squamous Cell Carcinoma In A Cow, Ali Baniadam, Navid Moezzi, Babak Mohammadian
Turkish Journal of Veterinary & Animal Sciences
A patient with a turgid, hemorrhagic mass infected by myiasis in the left nostril and diagnosed as squamous cell carcinoma (SCC) is presented. Complete blood count (CBC) showed moderate neutrophilia and eosinophilia and vital signs were in normal range. After aseptic surgical preparations and local anesthesia, the mass removed en bloc and submitted to the Department of Veterinary Pathology for histopathological evaluation. SCC was recognized microscopically by identifying malignant epithelial cells demonstrating various degrees of differentiation towards keratinocytes.
Impairments In Haematological Parameters Of Saprolegnia Spp. Infected Tench,Tinca Tinca,
2010
TÜBİTAK
Impairments In Haematological Parameters Of Saprolegnia Spp. Infected Tench,Tinca Tinca, Syed Lal Shah
Turkish Journal of Veterinary & Animal Sciences
Haematological parameters of tench (Tinca tinca) infected by Saprolegnia spp. were studied. All parameters, except mean corpuscular volume (MCV), decreased in response to infection. A significant (P < 0.05) decrease was observed in haemoglobin (Hb) (-26.85%), red blood cell count (RBCc) (-20.94%), leucocrit (Lct) (-24.14%), and mean corpuscular haemoglobin concentration (MCHC) (-9.06%). Haematocrit (Hct), white blood cell count (WBCc), and mean corpuscular haemoglobin (MCH) also decreased (-7.66%, -12.44%, and -6.19%, respectively); however, differences between the infected and control groups were not significant. Among the parameters, only MCV had a non-significant increase (+7.84%) in the infected fish. The study results show that saprolegniasis caused anaemia and immunosuppression, followed by mortality in tench. Mortality was attributed to haemodilution caused by haemorrhaging and the breakdown of osmotic balance due to tissue (skin and muscular layer) destruction via the penetration of the hyphae; however, lethargy was another mortality promoting factor. Mortality among the Saprolegnia-infected fish also depended upon the initial site of infection, type of tissue destroyed, growth rate of the fungus, and ability of individual fish to withstand the stress. The present study was conducted to investigate physiological impairment in Saprolegnia-infected Tinca tinca under laboratory conditions; however, further studies at the population level in natural habitats and under similar stress conditions are needed.
