Cloning Efficiency Of Canine Mesenchymal Stem Cells Isolated From Bone Marrow Of Femoral Head And Subcutaneous Adipose Tissue,
2013
Chulalongkorn University
Cloning Efficiency Of Canine Mesenchymal Stem Cells Isolated From Bone Marrow Of Femoral Head And Subcutaneous Adipose Tissue, Nada Tanamai, Sirirak Chantakru, Monchanok Vijarnsorn
The Thai Journal of Veterinary Medicine
Most recently, adipose tissue (AD) has become an alternative source for mesenchymal stem cells (MSCs) instead of the invasive method of bone marrow (BM) aspiration both in human and canine. In this study, we compared MSCs derived from adipose tissue (AD-MSCs) and bone marrow (BM-MSCs) regarding morphology and cell yield for instant usage by using the standard protocol of counting colony forming unit-fibroblast (CFU-F). MSCs from both sources showed fibroblast-like morphology and formed colonies termed as CFU-F after culturing in plastic surface for 10 days. The colony number per mononuclear cells (MNCs) and the colony number per adherent cells derived …
Statistical Study Of The Association Between Shelter Canine Intakes And Euthanasia From 2001 To 2011 In Taiwan,
2013
Chulalongkorn University
Statistical Study Of The Association Between Shelter Canine Intakes And Euthanasia From 2001 To 2011 In Taiwan, I-Feng Yen, Hsiu-I Lin, Dang-Jie Lan, Ting-Wei Chang, Kai Pan, Chang-Young Fei
The Thai Journal of Veterinary Medicine
This study used Pearson correlation test to analyze statistical correlation between shelter canine intake and number of dogs euthanized in municipal animal shelters in 20 counties/cities in Taiwan from 2001 to 2011. Data of animal shelters were official data provided by the Council of Agriculture, the competent authority of the Animal Protection Act in Taiwan. Among the 20 counties/cities in Taiwan, Hualien County, Penghu County, Keelung City, and Chiayi County were excluded from this study due to incomplete data. Pearson correlation test was used to analyze the data of other 16 counties/cities from 2001 to 2011. Among these 16 counties/cities, …
Effect Of Wild Cape Gooseberry (Physalis Minima Linn.) On Zebra Fish (Danio Rerio) Embryo,
2013
Chulalongkorn University
Effect Of Wild Cape Gooseberry (Physalis Minima Linn.) On Zebra Fish (Danio Rerio) Embryo, Aranya Ponpornpisit, Nopadon Pirarat, Wanwipa Suthikrai, Anong Binwihok
The Thai Journal of Veterinary Medicine
The effect of crude extract of wild cape gooseberry, P. minima Linn. on zebra fish, D. rerio, embryo was investigated. Five concentrations (0.01%, 0.1%, 1%, 10% and 20%) of an aqueous crude extract of the plant leaf was prepared and tested with newly spawned embryos in 96 well plates. The observation effect was based on the toxicity endpoint composed of lethal effects, sublethal effects and malformation effects appearing within 144 hours postfertilization (hpf). The results suggested that the lowest observed effect concentrations (LOEC) for a lethal effect and a sublethal effect were 20% and 10% respectively. Not fully developed pigmentation …
Ecg Quiz,
2013
Chulalongkorn University
Ecg Quiz, Chollada Buranakarl, Suwanakiet Sawangkoon, Chutamas Benjanirut, Monkon Trisiriroj, Thanusorn Phakhawambodee
The Thai Journal of Veterinary Medicine
No abstract provided.
Ophthalmology Snapshot,
2013
Chulalongkorn University
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
Ultrasound Diagnosis,
2013
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
What Is Your Diagnosis,
2013
Chulalongkorn University
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep, Panida Pongvittayanon
The Thai Journal of Veterinary Medicine
No abstract provided.
Morphological Aspects By Light And Scanning Electron Microscopic Studies Of Swamp Buffalo Endometrium At Follicular And Mid-Luteal Phases,
2013
Chulalongkorn University
Morphological Aspects By Light And Scanning Electron Microscopic Studies Of Swamp Buffalo Endometrium At Follicular And Mid-Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong
The Thai Journal of Veterinary Medicine
This study was carried out to observe morphological changes of swamp buffalo endometrium at follicularand mid-luteal phases. Uterine horns were collected from female buffaloes at a local abattoir and the selected estrousstages were categorized into the follicular (n = 10) and mid-luteal (n = 10) phases. General histology andhistomorphometry were examined under light microscope (LM) whereas a scanning electron microscope (SEM) wasused to study surface epithelial changes. The results showed that the height of the endometrial epithelium, thenumber of superficial endometrial glands and the number of capillaries were significantly greater (p < 0.05) at thefollicular phase. By SEM examination, the ciliated and secretory cells with different patterns, i.e. abundant microvillion the apical part or secretory protrusion in various degrees, distinctly increased at the follicular phase. In themeantime, numerous secretory cells with stubby microvilli were covered throughout the endometrial surface withsecretory vesicles on endometrial glandular orifices at the mid-luteal phase in which the ciliated cells were sparselyseen. It was concluded that the swamp buffalo endometrium obviously revealed modifications during the estrouscycle for physiological events, i.e. sperm transport, early embryonic development and implantation.
Subchronic To Chronic Pulmonary Effects Of Low Dose Of Diesel Exhaust Particles In Mice After Single Intratracheal Instillation: Pathological Changes And Metallothionien Responses,
2013
Chulalongkorn University
Subchronic To Chronic Pulmonary Effects Of Low Dose Of Diesel Exhaust Particles In Mice After Single Intratracheal Instillation: Pathological Changes And Metallothionien Responses, Theerayuth Kaewamatawong, Wijit Banlunara, Anong Bintvihok, Akinori Shimada
The Thai Journal of Veterinary Medicine
To study the subchronic and chronic lung effects of low dose of Diesel Exhaust Particles (DEPs), mice were intratracheally instilled with 50 μg of DEPs. Cellular and biochemical parameters in bronchoalveolar lavage fluid (BALF) and histological alteration were determined at 90, 120, 150 and 180 days after instillation. From BALF results, exposure to 50 μg of DEPs did not cause any pulmonary inflammation. However, mild to moderate pulmonary changes evidenced by multifocal DEPs laden alveolar macrophages (AMs) aggregation, type II alveolar cell proliferation, thickening of alveolar wall, interstitial fibrosis and edema were observed in treated mouse lungs. Basement (BM) damage …
Association Among Serum Insulin–Like Growth Factor–I, Backfat Thickness, And Age At First Observed Estrus In Gilts,
2013
Chulalongkorn University
Association Among Serum Insulin–Like Growth Factor–I, Backfat Thickness, And Age At First Observed Estrus In Gilts, Atthaporn Roongsitthichai, Seri Koonjaenak, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study investigated the association among serum insulin-like growth factor-I (IGF-I), backfatthickness, and age at first observed estrus in replacement gilts. In total, 80 replacement gilt, Landrace (L, n = 48) andYorkshire (Y, n = 32), were included. The gilts were determined for age at first observed estrus between 167 to 212days of age. They were categorized according to age at first observed estrus into ≤ 200 days (n = 41) and > 200 days (n= 36) groups. Backfat thickness, body weight, and serum IGF–I concentration were measured before mating. Age atfirst mating, interval from boar exposure to first observed …
Modulating Neurogenesis In Embryoid Body Using A Selective Tgf Beta1/Alk Inhibitor Affects Gene Expression Of Embryonic Stem Cell-Derived Motor Neurons,
2013
Chulalongkorn University
Modulating Neurogenesis In Embryoid Body Using A Selective Tgf Beta1/Alk Inhibitor Affects Gene Expression Of Embryonic Stem Cell-Derived Motor Neurons, Nuttha Klincumhom, Theerawat Tharasanit, Chommanart Thongkittidilok, Narong Tiptanavattana, Andras Dinnyes, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
Mouse embryonic stem (ES) cells have been served as potential model for investigation of underlying mechanisms at cellular and molecular levels of neurological disorders. The improvement of motor neuron differentiation is prospected to gain more understanding aimed at overcoming several incurable motor neuron diseases. In this study, we examined the effects of selective inhibitor of TGF-β type I receptor on the efficacy of neuronal differentiation of mouse embryonic stem cells toward motor neuron. Pluripotent ES cells were induced to form as EB in suspension medium supplemented with retinoic acid using -4/+4 protocol. Thereafter, 8-day old EBs were further induced to …
Differentiation Of Secreted Proteins From A Virulent And An Avirulent Strain Of Streptococcus Suis Serotype 2,
2013
Chulalongkorn University
Differentiation Of Secreted Proteins From A Virulent And An Avirulent Strain Of Streptococcus Suis Serotype 2, Xiao Wei Yang, Cui Wei Li1, Zhi Biao Yang, Li Ping Wu, Xiu Guo Hua
The Thai Journal of Veterinary Medicine
The present study aimed to identify specific and upregulated proteins in virulent SS2 strain that mightconfer pathogenicity. We performed a comparative proteomic assay to analyze the difference in secretome betweenvirulent SH-08 strain and avirulent SH-YH07 strain. Virulence of tested strains was determined using porcine andzebrafish models. The supernatant from bacterial cultures was collected at early exponential phase (OD600 = 0.4).Proteomic assay identified 52 proteins from the virulent strain supernatant and 29 proteins from the avirulent strainsupernatant. Twenty-three proteins were found in the culture medium from both strains. Among these commonproteins, eleven were upregulated by the virulent strain compared to the …
Acute To Subchronic Toxicity And Reproductive Effects Of Aqueous Ethanolic Extract Of Rhizomes Of Lasia Spinosa Thw. In Male Rats,
2013
Chulalongkorn University
Acute To Subchronic Toxicity And Reproductive Effects Of Aqueous Ethanolic Extract Of Rhizomes Of Lasia Spinosa Thw. In Male Rats, Theerayuth Kaewamatawong, Wanwipa Suthikrai, Anong Bintvihok, Wijit Banlunara
The Thai Journal of Veterinary Medicine
Acute and subchronic tests on the toxicity and reproductive effects of aqueous ethanolic extract of rhizomes of Lasia spinosa Thw. was studied in male rats. In the acute test, oral administration of 5, 10, 20, 40 g/kg of the extract showed no mortality or behavioral changes. In the subchronic test, administration of 5 or 20 g/kg of the extract for 28 days revealed no mortality of the animals. No differences in hematological parameters were observed in either control or treatment groups of both tests. For blood chemistry analysis in both tests, triglycerides in treatment rats were significantly decreased compared to …
The Role Of Kisspeptin Signaling In Reproduction Of Ruminant,
2013
Chulalongkorn University
The Role Of Kisspeptin Signaling In Reproduction Of Ruminant, Thuchadaporn Chaikhun, Pongsiwa Sotthibandhu, Siriwat Suadsong
The Thai Journal of Veterinary Medicine
In the present decade, kisspeptin has been a fascinating topic in the reproductive spotlight. Kisspeptin is a neuropeptide, produced mainly in the hypothalamus from Kiss1 gene. This gene was initially discovered in 1996 as a tumor suppressor gene in human malignant melanoma. Then in the year 2003, researchers found that mutations of the G protein-coupled receptor (GPR54), which is the strongly cognate receptor of kisspeptin, were associated with hypogonadotropic hypogonadism in mice and humans. There are many kisspeptin distribution and function studies (both in vitro and in vivo) in mammals. In ruminants, kisspeptin has been studied mainly in sheep and …
Sds-Page Electrophoresis For Urinary Protein Analysis In Dogs With Chronic Kidney Disease And Urinary Tract Infection,
2013
Chulalongkorn University
Sds-Page Electrophoresis For Urinary Protein Analysis In Dogs With Chronic Kidney Disease And Urinary Tract Infection, Orapun Jaturakan, Manisin Vanichwatanaramlouk, Arunrat Kornkaew, Monkon Trisiriroj, Winai Chansaisakorn, Siripen Komolvanich, Kittipong Tachampa, Chollada Buranakarl
The Thai Journal of Veterinary Medicine
The objective of the present study was to investigate urinary protein profiles in dogs with chronic kidney disease (CKD) in comparison to dogs with urinary tract infection (UTI). Animals were divided into 4 groups: control, CKD stages II+III, CKD stage IV and UTI. Blood pressure was measured using oscillometric method. Blood was collected for determinations of packed cell volume (PCV), blood urea nitrogen (BUN) and plasma creatinine concentrations. Urine was collected for urinalysis and protein determination. Total urinary proteins were measured using semi-quantitative method by precipitation with sulfosalicylic acid and a standard SDS-polyacrylamide gel electrophoresis (SDS-PAGE) which were presented as …
Mediating Trophoblast Uptake Of Methylaminoisobutyric Acid And Glutamine By Absence Of Extra- Or Intracellular Glutamine,
2013
Chulalongkorn University
Mediating Trophoblast Uptake Of Methylaminoisobutyric Acid And Glutamine By Absence Of Extra- Or Intracellular Glutamine, Boonrit Thongsong
The Thai Journal of Veterinary Medicine
Glutamine is known to modulate the uptake activity of nutrient transporters in mammalian cells. The aim of the study was to elucidate the influence of absence of extra- or intracellular glutamine on the uptake activity of the amino acid transport system A in placental BeWo cells. Extracellular glutamine was absent by culturing the cells in regular medium without glutamine. Intracellular glutamine was depleted by culturing the cells in regular medium without glutamine and by treating the cells for 16 hours with methionine sulfoximine (MSX), an inhibitor of glutamine synthetase. When BeWo cells were treated with glutamine-free culture medium for 1-30 …
A Survey To Determine The Presence Of The N-Acetylglucosamine-6-Sulfatase (G6s) Gene Mutation In Anglo-Nubian Goats In Southern Thailand,
2013
Chulalongkorn University
A Survey To Determine The Presence Of The N-Acetylglucosamine-6-Sulfatase (G6s) Gene Mutation In Anglo-Nubian Goats In Southern Thailand, Siriwat Wasiksiri, Sirichai Sripongpun, Adisorn Ratanaphan, Panom Sookras
The Thai Journal of Veterinary Medicine
Mutation of the G6S gene in Anglo-Nubian (AN) goat causes non-function of the N-acetylglucosamide 6- sulfatase enzyme within lysosomes, which increases heparin sulfate accumulation and induces pathogenesis of the internal organs. Transversion of base C to T at nucleotide 322 changes decoding of an amino arginine to a stop codon (R322X) and produces a truncated protein. This mutation is detrimental to the health of a goat kid leading to delay in nervous development and death. It was found first time in goats of a Michigan herd in the US, but has not yet been reported in Thailand. This study aimed …
Role Of An Iron-Dependent Transcriptional Regulator In The Pathogenesis And Host Response To Infection With Streptococcus Pneumoniae.,
2013
Mississippi State University
Role Of An Iron-Dependent Transcriptional Regulator In The Pathogenesis And Host Response To Infection With Streptococcus Pneumoniae., Radha Gupta, Minny Bhatty, Edwin Swiatlo, Bindu Nanduri
CVM Publications
Iron is a critical cofactor for many enzymes and is known to regulate gene expression in many bacterial pathogens. Streptococcus pneumoniae normally inhabits the upper respiratory mucosa but can also invade and replicate in lungs and blood. These anatomic sites vary considerably in both the quantity and form of available iron. The genome of serotype 4 pneumococcal strain TIGR4 encodes a putative iron-dependent transcriptional regulator (IDTR). A mutant deleted at idtr (?idtr) exhibited growth kinetics similar to parent strain TIGR4 in vitro and in mouse blood for up to 48 hours following infection. However, ?idtr was significantly attenuated in a …
Neutering Dogs: Effects On Joint Disorders And Cancers In Golden Retrievers,
2013
University of California, Davis
Neutering Dogs: Effects On Joint Disorders And Cancers In Golden Retrievers, Gretel Torres De La Riva, Benjamin L. Hart, Thomas B. Farver, Anita M. Oberbauer, Locksley L. Mcv Messam, Neil H. Willits, Lynette A. Hart
Endocrinology Collection
In contrast to European countries, the overwhelming majority of dogs in the U.S. are neutered (including spaying), usually done before one year of age. Given the importance of gonadal hormones in growth and development, this cultural contrast invites an analysis of the multiple organ systems that may be adversely affected by neutering. Using a single breed-specific dataset, the objective was to examine the variables of gender and age at the time of neutering versus leaving dogs gonadally intact, on all diseases occurring with sufficient frequency for statistical analyses. Given its popularity and vulnerability to various cancers and joint disorders, the …
The Herpesvirus Vp1/2 Protein Is An Effector Of Dynein-Mediated
Capsid Transport And Neuroinvasion,
2013
Northwestern University Feinberg School of Medicine
The Herpesvirus Vp1/2 Protein Is An Effector Of Dynein-Mediated Capsid Transport And Neuroinvasion, Sofia V. Zaichick, Kevin P. Bohannon, Ami Hughes, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith
School of Veterinary and Biomedical Sciences: Faculty Publications
Microtubule transport of herpesvirus capsids from the cell periphery to the nucleus is imperative for viral replication and, in the case of many alphaherpesviruses, transmission into the nervous system. Using the neuroinvasive herpesvirus, pseudorabies virus (PRV), we show that the viral protein 1/2 (VP1/2) tegument protein associates with the dynein/dynactin microtubule motor complex and promotes retrograde microtubule transport of PRV capsids. Functional activation of VP1/2 requires binding to the capsid protein pUL25 or removal of the capsid-binding domain. A proline-rich sequence within VP1/2 is required for the efficient interaction with the dynein/ dynactin microtubule motor complex as well as for …
