Association Among Serum Insulin–Like Growth Factor–I, Backfat Thickness, And Age At First Observed Estrus In Gilts,
2013
Chulalongkorn University
Association Among Serum Insulin–Like Growth Factor–I, Backfat Thickness, And Age At First Observed Estrus In Gilts, Atthaporn Roongsitthichai, Seri Koonjaenak, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study investigated the association among serum insulin-like growth factor-I (IGF-I), backfatthickness, and age at first observed estrus in replacement gilts. In total, 80 replacement gilt, Landrace (L, n = 48) andYorkshire (Y, n = 32), were included. The gilts were determined for age at first observed estrus between 167 to 212days of age. They were categorized according to age at first observed estrus into ≤ 200 days (n = 41) and > 200 days (n= 36) groups. Backfat thickness, body weight, and serum IGF–I concentration were measured before mating. Age atfirst mating, interval from boar exposure to first observed …
Sds-Page Electrophoresis For Urinary Protein Analysis In Dogs With Chronic Kidney Disease And Urinary Tract Infection,
2013
Chulalongkorn University
Sds-Page Electrophoresis For Urinary Protein Analysis In Dogs With Chronic Kidney Disease And Urinary Tract Infection, Orapun Jaturakan, Manisin Vanichwatanaramlouk, Arunrat Kornkaew, Monkon Trisiriroj, Winai Chansaisakorn, Siripen Komolvanich, Kittipong Tachampa, Chollada Buranakarl
The Thai Journal of Veterinary Medicine
The objective of the present study was to investigate urinary protein profiles in dogs with chronic kidney disease (CKD) in comparison to dogs with urinary tract infection (UTI). Animals were divided into 4 groups: control, CKD stages II+III, CKD stage IV and UTI. Blood pressure was measured using oscillometric method. Blood was collected for determinations of packed cell volume (PCV), blood urea nitrogen (BUN) and plasma creatinine concentrations. Urine was collected for urinalysis and protein determination. Total urinary proteins were measured using semi-quantitative method by precipitation with sulfosalicylic acid and a standard SDS-polyacrylamide gel electrophoresis (SDS-PAGE) which were presented as …
Effect Of L-Cysteine On Chilled Carp (Cyprinus Carpio) Semen Qualities,
2013
Chulalongkorn University
Effect Of L-Cysteine On Chilled Carp (Cyprinus Carpio) Semen Qualities, Kan Kledmanee, Somrat Taweedet, Prawporn Thaijongruk, Panida Chanapiwat, Kampon Kaeoket
The Thai Journal of Veterinary Medicine
The aim of the present study was to study the effects of L-cysteine on chilled carp (Cyprinus carpio) semen qualities. Pooled semen samples were prepared from eight fish, and divided into five groups according to the concentrations of L-cysteine as follows: 0 (T1), 0.5 (T2), 1.0 (T3), 1.5 (T4) and 2 (T5) mM. The sperm motility, duration of sperm motility and sperm viability were evaluated at 0, 12, 24, 48, 72 hours after chilled storage. Comparing between treatment and control groups, the percentage of sperm motility, duration of sperm motility and the percentage of sperm viability in the treatment groups …
A Survey To Determine The Presence Of The N-Acetylglucosamine-6-Sulfatase (G6s) Gene Mutation In Anglo-Nubian Goats In Southern Thailand,
2013
Chulalongkorn University
A Survey To Determine The Presence Of The N-Acetylglucosamine-6-Sulfatase (G6s) Gene Mutation In Anglo-Nubian Goats In Southern Thailand, Siriwat Wasiksiri, Sirichai Sripongpun, Adisorn Ratanaphan, Panom Sookras
The Thai Journal of Veterinary Medicine
Mutation of the G6S gene in Anglo-Nubian (AN) goat causes non-function of the N-acetylglucosamide 6- sulfatase enzyme within lysosomes, which increases heparin sulfate accumulation and induces pathogenesis of the internal organs. Transversion of base C to T at nucleotide 322 changes decoding of an amino arginine to a stop codon (R322X) and produces a truncated protein. This mutation is detrimental to the health of a goat kid leading to delay in nervous development and death. It was found first time in goats of a Michigan herd in the US, but has not yet been reported in Thailand. This study aimed …
Histomorphometrical Variations Of Hair Follicles Of Bakhtiari Sheep At Different Skin Areas,
2013
Chulalongkorn University
Histomorphometrical Variations Of Hair Follicles Of Bakhtiari Sheep At Different Skin Areas, Behzad Mobini
The Thai Journal of Veterinary Medicine
The purpose of this study was to determine the histomorphometrical variations of hair follicles at different skin areas in Bakhtiari sheep. Twelve healthy adult sheep (6 males and 6 females) of the Bakhtiari breed, aged from 1 to 2 years, were selected from the abattoir of Shahrekord. Skin samples were taken from different areas, fixed and stained with haematoxylin and eosin. By using lattice line graticule (5x5) and ocular micrometer, the following histomorphometric indices were tested: number of primary and secondary follicles per one mm2; depth and width of primary and secondary follicles; ratios of number, depth and width of …
Survey Of Toxoplasma Gondii In Taipei: Livestock Meats, Internal Organs, Cat And Dog Sera,
2013
Chulalongkorn University
Survey Of Toxoplasma Gondii In Taipei: Livestock Meats, Internal Organs, Cat And Dog Sera, Ying-Bin Taiching Fuh, Albert Taiching Liao, Yun-Ming Taiching Pong, Meng-Chih Taiching Tung, Chang-Young Taiching Fei, Dah-Sheng Lin
The Thai Journal of Veterinary Medicine
Toxoplasma gondii (T. gondii) is an important food-borne zoonotic protozoa. Infection occurs when humans and warm-blooded animals consume raw or lightly cooked cysts containing meat or sporulated oocysts contaminated food. T. gondii infection has been observed in food animals in many countries suggesting the significance of food animals in the epidemiology of toxoplasmosis. However, there is no report regarding T. gondii infection in food animals other than pigs in Taiwan. Therefore, in this study, pork, pig livers, mutton, chicken flesh, chicken hearts, chicken livers, gizzards, and imported beef sold in the supermarkets in Taipei were examined for T. gondii deoxyribonucleic …
What Is Your Diagnosis,
2013
Chulalongkorn University
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep, Panida Pongvittayanon
The Thai Journal of Veterinary Medicine
No abstract provided.
Subchronic To Chronic Pulmonary Effects Of Low Dose Of Diesel Exhaust Particles In Mice After Single Intratracheal Instillation: Pathological Changes And Metallothionien Responses,
2013
Chulalongkorn University
Subchronic To Chronic Pulmonary Effects Of Low Dose Of Diesel Exhaust Particles In Mice After Single Intratracheal Instillation: Pathological Changes And Metallothionien Responses, Theerayuth Kaewamatawong, Wijit Banlunara, Anong Bintvihok, Akinori Shimada
The Thai Journal of Veterinary Medicine
To study the subchronic and chronic lung effects of low dose of Diesel Exhaust Particles (DEPs), mice were intratracheally instilled with 50 μg of DEPs. Cellular and biochemical parameters in bronchoalveolar lavage fluid (BALF) and histological alteration were determined at 90, 120, 150 and 180 days after instillation. From BALF results, exposure to 50 μg of DEPs did not cause any pulmonary inflammation. However, mild to moderate pulmonary changes evidenced by multifocal DEPs laden alveolar macrophages (AMs) aggregation, type II alveolar cell proliferation, thickening of alveolar wall, interstitial fibrosis and edema were observed in treated mouse lungs. Basement (BM) damage …
Modulating Neurogenesis In Embryoid Body Using A Selective Tgf Beta1/Alk Inhibitor Affects Gene Expression Of Embryonic Stem Cell-Derived Motor Neurons,
2013
Chulalongkorn University
Modulating Neurogenesis In Embryoid Body Using A Selective Tgf Beta1/Alk Inhibitor Affects Gene Expression Of Embryonic Stem Cell-Derived Motor Neurons, Nuttha Klincumhom, Theerawat Tharasanit, Chommanart Thongkittidilok, Narong Tiptanavattana, Andras Dinnyes, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
Mouse embryonic stem (ES) cells have been served as potential model for investigation of underlying mechanisms at cellular and molecular levels of neurological disorders. The improvement of motor neuron differentiation is prospected to gain more understanding aimed at overcoming several incurable motor neuron diseases. In this study, we examined the effects of selective inhibitor of TGF-β type I receptor on the efficacy of neuronal differentiation of mouse embryonic stem cells toward motor neuron. Pluripotent ES cells were induced to form as EB in suspension medium supplemented with retinoic acid using -4/+4 protocol. Thereafter, 8-day old EBs were further induced to …
Acute To Subchronic Toxicity And Reproductive Effects Of Aqueous Ethanolic Extract Of Rhizomes Of Lasia Spinosa Thw. In Male Rats,
2013
Chulalongkorn University
Acute To Subchronic Toxicity And Reproductive Effects Of Aqueous Ethanolic Extract Of Rhizomes Of Lasia Spinosa Thw. In Male Rats, Theerayuth Kaewamatawong, Wanwipa Suthikrai, Anong Bintvihok, Wijit Banlunara
The Thai Journal of Veterinary Medicine
Acute and subchronic tests on the toxicity and reproductive effects of aqueous ethanolic extract of rhizomes of Lasia spinosa Thw. was studied in male rats. In the acute test, oral administration of 5, 10, 20, 40 g/kg of the extract showed no mortality or behavioral changes. In the subchronic test, administration of 5 or 20 g/kg of the extract for 28 days revealed no mortality of the animals. No differences in hematological parameters were observed in either control or treatment groups of both tests. For blood chemistry analysis in both tests, triglycerides in treatment rats were significantly decreased compared to …
The Role Of Kisspeptin Signaling In Reproduction Of Ruminant,
2013
Chulalongkorn University
The Role Of Kisspeptin Signaling In Reproduction Of Ruminant, Thuchadaporn Chaikhun, Pongsiwa Sotthibandhu, Siriwat Suadsong
The Thai Journal of Veterinary Medicine
In the present decade, kisspeptin has been a fascinating topic in the reproductive spotlight. Kisspeptin is a neuropeptide, produced mainly in the hypothalamus from Kiss1 gene. This gene was initially discovered in 1996 as a tumor suppressor gene in human malignant melanoma. Then in the year 2003, researchers found that mutations of the G protein-coupled receptor (GPR54), which is the strongly cognate receptor of kisspeptin, were associated with hypogonadotropic hypogonadism in mice and humans. There are many kisspeptin distribution and function studies (both in vitro and in vivo) in mammals. In ruminants, kisspeptin has been studied mainly in sheep and …
Morphology Of Brain Waveforms And Electrical Activity Of Visual Evoked Potentials (Vep) Of Iguana Iguana Species Under Captivity Conditions In Mexico,
2013
Chulalongkorn University
Morphology Of Brain Waveforms And Electrical Activity Of Visual Evoked Potentials (Vep) Of Iguana Iguana Species Under Captivity Conditions In Mexico, Salvador Abraham Solís-Chávez, Alejandra Ibáñez-Contreras, Alfredo Durand-Rivera, Sergio A. Reyes-Pantoja, Alfaro Rodríguez Alfonso, Eduardo Tena-Betancourt, Braulio Hernández-Godínez
The Thai Journal of Veterinary Medicine
Reptiles have been characterized by relevant number of papers as well-known animal models in biomedical research and neuroethology due to their ancestral evolutionary relationship with birds and mammals, leading to further hypothesis that the dorsal ventricular ridge (DVR), the dorsal pallia of amphibians and the isocortex of mammalian brains share a common anatomical origin. The objective of this study was to assess the morphologic features and measurement of brain waveforms of Iguana iguana through Visual Evoked Potentials (VEP). This research employed six healthy and ophthalmologically-suited iguanas (Iguana iguana), with computerized tomography brain scans followed by VEP under simple temporary physical …
Dha Analysis In Different Types Of Egg Yolks: Its Possibility Of Being A Dha Source For Boar Semen Cryopreservation,
2013
Chulalongkorn University
Dha Analysis In Different Types Of Egg Yolks: Its Possibility Of Being A Dha Source For Boar Semen Cryopreservation, Kampon Kaeoket, Panida Chanapiwat
The Thai Journal of Veterinary Medicine
The aim of this study was to investigate the concentrations of Docosahexaenoic acid (DHA) in DHA enriched hen egg yolk (H-DHA) in comparison with egg yolk from chicken, duck, quail, ostrich and crocodile in order to find a source of DHA for boar semen cryopreservation. A pool (10 eggs in each) of DHA enriched hen egg yolk and egg yolks from chicken, duck, quail, ostrich and crocodile were analyzed for fatty acids profile at the Nutrition Laboratory, Mahidol University. Comparing all egg yolks, the highest DHA concentration in egg yolk was found in H-DHA egg yolk (3.7% of total fatty …
Cloning Efficiency Of Canine Mesenchymal Stem Cells Isolated From Bone Marrow Of Femoral Head And Subcutaneous Adipose Tissue,
2013
Chulalongkorn University
Cloning Efficiency Of Canine Mesenchymal Stem Cells Isolated From Bone Marrow Of Femoral Head And Subcutaneous Adipose Tissue, Nada Tanamai, Sirirak Chantakru, Monchanok Vijarnsorn
The Thai Journal of Veterinary Medicine
Most recently, adipose tissue (AD) has become an alternative source for mesenchymal stem cells (MSCs) instead of the invasive method of bone marrow (BM) aspiration both in human and canine. In this study, we compared MSCs derived from adipose tissue (AD-MSCs) and bone marrow (BM-MSCs) regarding morphology and cell yield for instant usage by using the standard protocol of counting colony forming unit-fibroblast (CFU-F). MSCs from both sources showed fibroblast-like morphology and formed colonies termed as CFU-F after culturing in plastic surface for 10 days. The colony number per mononuclear cells (MNCs) and the colony number per adherent cells derived …
Statistical Study Of The Association Between Shelter Canine Intakes And Euthanasia From 2001 To 2011 In Taiwan,
2013
Chulalongkorn University
Statistical Study Of The Association Between Shelter Canine Intakes And Euthanasia From 2001 To 2011 In Taiwan, I-Feng Yen, Hsiu-I Lin, Dang-Jie Lan, Ting-Wei Chang, Kai Pan, Chang-Young Fei
The Thai Journal of Veterinary Medicine
This study used Pearson correlation test to analyze statistical correlation between shelter canine intake and number of dogs euthanized in municipal animal shelters in 20 counties/cities in Taiwan from 2001 to 2011. Data of animal shelters were official data provided by the Council of Agriculture, the competent authority of the Animal Protection Act in Taiwan. Among the 20 counties/cities in Taiwan, Hualien County, Penghu County, Keelung City, and Chiayi County were excluded from this study due to incomplete data. Pearson correlation test was used to analyze the data of other 16 counties/cities from 2001 to 2011. Among these 16 counties/cities, …
Effect Of Wild Cape Gooseberry (Physalis Minima Linn.) On Zebra Fish (Danio Rerio) Embryo,
2013
Chulalongkorn University
Effect Of Wild Cape Gooseberry (Physalis Minima Linn.) On Zebra Fish (Danio Rerio) Embryo, Aranya Ponpornpisit, Nopadon Pirarat, Wanwipa Suthikrai, Anong Binwihok
The Thai Journal of Veterinary Medicine
The effect of crude extract of wild cape gooseberry, P. minima Linn. on zebra fish, D. rerio, embryo was investigated. Five concentrations (0.01%, 0.1%, 1%, 10% and 20%) of an aqueous crude extract of the plant leaf was prepared and tested with newly spawned embryos in 96 well plates. The observation effect was based on the toxicity endpoint composed of lethal effects, sublethal effects and malformation effects appearing within 144 hours postfertilization (hpf). The results suggested that the lowest observed effect concentrations (LOEC) for a lethal effect and a sublethal effect were 20% and 10% respectively. Not fully developed pigmentation …
Ultrasound Diagnosis,
2013
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Differentiation Of Secreted Proteins From A Virulent And An Avirulent Strain Of Streptococcus Suis Serotype 2,
2013
Chulalongkorn University
Differentiation Of Secreted Proteins From A Virulent And An Avirulent Strain Of Streptococcus Suis Serotype 2, Xiao Wei Yang, Cui Wei Li1, Zhi Biao Yang, Li Ping Wu, Xiu Guo Hua
The Thai Journal of Veterinary Medicine
The present study aimed to identify specific and upregulated proteins in virulent SS2 strain that mightconfer pathogenicity. We performed a comparative proteomic assay to analyze the difference in secretome betweenvirulent SH-08 strain and avirulent SH-YH07 strain. Virulence of tested strains was determined using porcine andzebrafish models. The supernatant from bacterial cultures was collected at early exponential phase (OD600 = 0.4).Proteomic assay identified 52 proteins from the virulent strain supernatant and 29 proteins from the avirulent strainsupernatant. Twenty-three proteins were found in the culture medium from both strains. Among these commonproteins, eleven were upregulated by the virulent strain compared to the …
Mediating Trophoblast Uptake Of Methylaminoisobutyric Acid And Glutamine By Absence Of Extra- Or Intracellular Glutamine,
2013
Chulalongkorn University
Mediating Trophoblast Uptake Of Methylaminoisobutyric Acid And Glutamine By Absence Of Extra- Or Intracellular Glutamine, Boonrit Thongsong
The Thai Journal of Veterinary Medicine
Glutamine is known to modulate the uptake activity of nutrient transporters in mammalian cells. The aim of the study was to elucidate the influence of absence of extra- or intracellular glutamine on the uptake activity of the amino acid transport system A in placental BeWo cells. Extracellular glutamine was absent by culturing the cells in regular medium without glutamine. Intracellular glutamine was depleted by culturing the cells in regular medium without glutamine and by treating the cells for 16 hours with methionine sulfoximine (MSX), an inhibitor of glutamine synthetase. When BeWo cells were treated with glutamine-free culture medium for 1-30 …
Role Of An Iron-Dependent Transcriptional Regulator In The Pathogenesis And Host Response To Infection With Streptococcus Pneumoniae.,
2013
Mississippi State University
Role Of An Iron-Dependent Transcriptional Regulator In The Pathogenesis And Host Response To Infection With Streptococcus Pneumoniae., Radha Gupta, Minny Bhatty, Edwin Swiatlo, Bindu Nanduri
CVM Publications
Iron is a critical cofactor for many enzymes and is known to regulate gene expression in many bacterial pathogens. Streptococcus pneumoniae normally inhabits the upper respiratory mucosa but can also invade and replicate in lungs and blood. These anatomic sites vary considerably in both the quantity and form of available iron. The genome of serotype 4 pneumococcal strain TIGR4 encodes a putative iron-dependent transcriptional regulator (IDTR). A mutant deleted at idtr (?idtr) exhibited growth kinetics similar to parent strain TIGR4 in vitro and in mouse blood for up to 48 hours following infection. However, ?idtr was significantly attenuated in a …
