Buccal Swab As A Source Of Noninvasive Technique For Genomic Dna Collection In Felidae,
2013
Chulalongkorn University
Buccal Swab As A Source Of Noninvasive Technique For Genomic Dna Collection In Felidae, Janjira Phavaphutanon, Sudtisa Laopium, Kawin Nanklang, Kaikanoke Sirinarumitr, Kornchai Kornkaewrat, Anuchai Pinyopummin, Jitrakorn Viriyarumpa, Piyawan Suthanmapinunt, Narathip Vorawattanatham
The Thai Journal of Veterinary Medicine
This study was conducted to determine whether buccal cells obtained by swabbing could be a DNA source for genetic analysis, and to compare two preservative media, distilled water and Tris-EDTA (TE), pH 8.0. Buccal cell samples were collected from 30 domestic cats (Felis catus), 4 captive fishing cats (Prionailurus viverrinus) and 8 captive tigers (Panthera tigris). The total concentration of DNA and purity were not statistically different between the preservative media. All samples could be graded as a minimum as high quality DNA (DNA found with A260/A280 ratio >2.0 or < 1.8). In addition, the quality of DNA extracted from both media was successfully amplified for PCR. However, when compared to whole blood samples, total DNA concentration from buccal swab was significantly lower (p < 0.05) .Despite the decrease in DNA yield, buccal swab could be an alternative source for obtaining DNA for genetic analyses. This non-invasive, simple and inexpensive technique eases genetic studies in this family especially in domestic cat from which whole blood samples are difficult to collect.
Ecg Quiz,
2013
Chulalongkorn University
Ecg Quiz, Chollada Buranakarl, Suwanakiet Sawangkoon, Anusak Kijtawornrat, Thanusorn Phakhawambodee
The Thai Journal of Veterinary Medicine
No abstract provided.
Ophthalmology Snapshot,
2013
Chulalongkorn University
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
Ultrasound Diagnosis,
2013
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Effects Of Environmental Factors, Ages And Breeds On Semen Characteristics In Thai Indigenous Chickens: A One-Year Study,
2013
Chulalongkorn University
Effects Of Environmental Factors, Ages And Breeds On Semen Characteristics In Thai Indigenous Chickens: A One-Year Study, Pornjit Sonseeda, Thevin Vongpralub, Banyat Laopaiboon
The Thai Journal of Veterinary Medicine
Several reports on the semen characteristics of domestic fowl have indicated that environmental factors, breed and age significantly affect semen quality. There is, however, a paucity of data on effects of environmental factor, breed and age on semen characteristic in Thai indigenous chickens. The aims of the present study were to investigate the effects of annual ambient temperature and humidity, local breeds (i.e. Lueng hang kaow, Pradoo hang dam and Chee), and ages (10 and 18 months) on sperm production and semen quality among Thai native cocks. Thirty-six Thai native cocks were housed under natural environmental conditions, and semen was …
What Is Your Diagnosis,
2013
Chulalongkorn University
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep1
The Thai Journal of Veterinary Medicine
No abstract provided.
Structural, Antigenic, And Evolutionary Characterizations Of The Envelope Protein Of Newly Emerging Duck Tembusu Virus,
2013
Yangzhou University
Structural, Antigenic, And Evolutionary Characterizations Of The Envelope Protein Of Newly Emerging Duck Tembusu Virus, Kexoang Yu, Zhi-Zhang Sheng, Bing Huang, Xiuli Ma, Yufeng Li, Xiaoyuam Yuan, Zhuoming Qin, Dan Wang, Suvobrata Chakravarty, Feng Li, Minxun Song, Huaichang Sun
Chemistry and Biochemistry Faculty Publications
Since the first reported cases of ducks infected with a previously unknown flavivirus in eastern China in April 2010, the virus, provisionally designated Duck Tembusu Virus (DTMUV), has spread widely in domestic ducks in China and caused significant economic losses to poultry industry. In this study, we examined in detail structural, antigenic, and evolutionary properties of envelope (E) proteins of six DTMUV isolates spanning 2010–2012, each being isolated from individual farms with different geographical locations where disease outbreaks were documented. Structural analysis showed that E proteins of DTMUV and its closely related flavivirus (Japanese Encephalitis Virus) shared a conserved array …
Mimicry Epitope From Ehrlichia Canis For Interphotoreceptor Retinoid-Binding Protein 201–216 Prevents Autoimmune Uveoretinitis By Acting As Altered Peptide Ligand,
2013
University of Nebraska-Lincoln
Mimicry Epitope From Ehrlichia Canis For Interphotoreceptor Retinoid-Binding Protein 201–216 Prevents Autoimmune Uveoretinitis By Acting As Altered Peptide Ligand, Arunakumar Gangaplara, Chandirasegaran Massilamany, David Steffen, Jay Reddy
Jay Reddy Publications
We report here identification of novel mimicry epitopes for interphotoreceptor retinoid-binding protein (IRBP) 201–216, a candidate ocular antigen that causes experimental autoimmune uveoretinitis (EAU) in A/J mice. One mimicry epitope from Ehrlichia canis (EHC), designated EHC 44–59, induced cross-reactive T cells for IRBP 201–216 capable of producing T helper (Th)1 and Th17 cytokines, but failed to induce EAU in A/J mice. In addition, animals first primed with suboptimal doses of IRBP 201–216 and subsequently immunized with EHC 44–59 did not develop EAU; rather, the mimicry epitope prevented the disease induced by IRBP 201–216. However, alteration in the composition of EHC …
Osmotic Stress, Not Aldose Reductase Activity, Directly Induces Growth Factors And Mapk Signaling Changes During Sugar Cataract Formation,
2013
University of Nebraska Medical Center
Osmotic Stress, Not Aldose Reductase Activity, Directly Induces Growth Factors And Mapk Signaling Changes During Sugar Cataract Formation, Peng Zhang, Kuiyi Xing, James Randazzo, Karen Blessing, Marjorie F. Lou, Peter Kador
School of Veterinary and Biomedical Sciences: Faculty Publications
In sugar cataract formation in rats, aldose reductase (AR) actitvity is not only linked to lenticular sorbitol (diabetic) or galactitol (galactosemic) formation but also to signal transduction changes, cytotoxic signals and activation of apoptosis. Using both in vitro and in vivo techniques, the interrelationship between AR activity, polyol (sorbitol and galactitol) formation, osmotic stress, growth factor induction, and cell signaling changes have been investigated. For in vitro studies, lenses from Sprague Dawley rats were cultured for up to 48 hrs in TC-199-bicarbonate media containing either 30 mM fructose (control), or 30 mM glucose or galctose with/without the aldose reductase inhibitors …
Analysis Of Unannotated Equine Transcripts Identified By Mrna Sequencing,
2013
University of Kentucky
Analysis Of Unannotated Equine Transcripts Identified By Mrna Sequencing, Stephen J. Coleman, Zheng Zeng, Matthew S. Hestand, Jinze Liu, James N. Macleod
Veterinary Science Faculty Publications
Sequencing of equine mRNA (RNA-seq) identified 428 putative transcripts which do not map to any previously annotated or predicted horse genes. Most of these encode the equine homologs of known protein-coding genes described in other species, yet the potential exists to identify novel and perhaps equine-specific gene structures. A set of 36 transcripts were prioritized for further study by filtering for levels of expression (depth of RNA-seq read coverage), distance from annotated features in the equine genome, the number of putative exons, and patterns of gene expression between tissues. From these, four were selected for further investigation based on predicted …
A Joint Matrix Completion And Filtering Model For Influenza Serological Data Integration.,
2013
Mississippi State University
A Joint Matrix Completion And Filtering Model For Influenza Serological Data Integration., Xiao-Tong Yuan, Tong Zhang, Xiu-Feng Wan
CVM Publications
Antigenic characterization based on serological data, such as Hemagglutination Inhibition (HI) assay, is one of the routine procedures for influenza vaccine strain selection. In many cases, it would be impossible to measure all pairwise antigenic correlations between testing antigens and reference antisera in each individual experiment. Thus, we have to combine and integrate the HI tables from a number of individual experiments. Measurements from different experiments may be inconsistent due to different experimental conditions. Consequently we will observe a matrix with missing data and possibly inconsistent measurements. In this paper, we develop a new mathematical model, which we refer to …
Heterogeneous Nuclear Ribonucleoprotein K Supports Vesicular
Stomatitis Virus Replication By Regulating Cell Survival And Cellular
Gene Expression,
2013
University of Nebraska-Lincoln
Heterogeneous Nuclear Ribonucleoprotein K Supports Vesicular Stomatitis Virus Replication By Regulating Cell Survival And Cellular Gene Expression, Phat X. Dinh, Anshuman Das, Rodrigo Franco, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a member of the family of hnRNPs and was recently shown in a genome-wide small interfering RNA (siRNA) screen to support vesicular stomatitis virus (VSV) growth. To decipher the role of hnRNP K in VSV infection, we conducted studies which suggest that the protein is required for VSV spreading. Virus binding to cells, entry, and nucleocapsid uncoating steps were not adversely affected in the absence of hnRNP K, whereas viral genome transcription and replication were reduced slightly. These results indicate that hnRNP K is likely involved in virus assembly and/or release from …
Growing South Dakota (Summer 2013),
2013
South Dakota State University
Growing South Dakota (Summer 2013), College Of Agriculture &. Biological Sciences
Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)
[Page] 2 Fish, Field & Forest: Natural Resource Management Research Covers the Gamut
[Page] 5 Profiles In Leadership: David Willis; Combined Department Creates Interdisciplinary Opportunities
[Page] 6 Research On the Range: Cottonwood Field Station Has Provided Research Data for 106 Years
[Page] 8 Prairie Oasis: Natural Diversity Showcased at Oak Lake Field Station
[Page] 9 Special Section: Investigating in Agriculture & Biological Sciences at SDSU: An Update On the College of Ag’s Enhanced Facilities and Continuing Efforts Important to Helping Address Future Agricultural Challenges and Opportunities.[Page] 13 South Dakota 4-H Update: Skill-a-thons, New Exhibit Hall Planned
[Page] 14 After The …
Envelope Determinants Of Equine Lentiviral Vaccine Protection,
2013
University of Pittsburgh - Main Campus
Envelope Determinants Of Equine Lentiviral Vaccine Protection, Jodi K. Craigo, Corin Ezzelarab, Sheila J. Cook, Chong Liu, David W. Horohov, Charles J. Issel, Ronald C. Montelaro
Veterinary Science Faculty Publications
Lentiviral envelope (Env) antigenic variation and associated immune evasion present major obstacles to vaccine development. The concept that Env is a critical determinant for vaccine efficacy is well accepted, however defined correlates of protection associated with Env variation have yet to be determined. We reported an attenuated equine infectious anemia virus (EIAV) vaccine study that directly examined the effect of lentiviral Env sequence variation on vaccine efficacy. The study identified a significant, inverse, linear correlation between vaccine efficacy and increasing divergence of the challenge virus Env gp90 protein compared to the vaccine virus gp90. The report demonstrated approximately 100% protection …
Tricarboxylic Acid Cycle And One-Carbon Metabolism Pathways Are Important In Edwardsiella Ictaluri Virulence.,
2013
Mississippi State University
Tricarboxylic Acid Cycle And One-Carbon Metabolism Pathways Are Important In Edwardsiella Ictaluri Virulence., Neeti Dahal, Hossam Abdelhamed, Jingjun Lu, Attila Karsi, Mark L. Lawrence
CVM Publications
Edwardsiella ictaluri is a Gram-negative facultative intracellular pathogen causing enteric septicemia of channel catfish (ESC). The disease causes considerable economic losses in the commercial catfish industry in the United States. Although antibiotics are used as feed additive, vaccination is a better alternative for prevention of the disease. Here we report the development and characterization of novel live attenuated E. ictaluri mutants. To accomplish this, several tricarboxylic acid cycle (sdhC, mdh, and frdA) and one-carbon metabolism genes (gcvP and glyA) were deleted in wild type E. ictaluri strain 93-146 by allelic exchange. Following bioluminescence tagging of the E. ictaluri ?sdhC, ?mdh, …
Indices Of Myocardial Contractility,
2013
Chulalongkorn University
Indices Of Myocardial Contractility, Anusak Kijtawornrat
The Thai Journal of Veterinary Medicine
Myocardial contractility is defined as the velocity of cycling of heavy meromyosin cross-bridges. Contractility may be defined also as the velocity of myocardial fiber shortening or change in ventricular dimension during contraction when the fiber shortens or the ventricle contracts against no load. In the clinic; however, myocardial contractility may be estimated by some parameters of systolic function adjusted for the degree of stretch on the fibers (the preload) just before contraction begins and the hindrance to ejection of blood (the afterload). Excitation-contraction coupling is a physicochemical process during which electrophysiological changes (initiated by depolarization of the cell) translate to …
Probable Pig To Duck Transmission Of The Pandemic H1n1 2009 (Ph1n1) And Its Reassortant In Commingling Experimental Condition,
2013
Chulalongkorn University
Probable Pig To Duck Transmission Of The Pandemic H1n1 2009 (Ph1n1) And Its Reassortant In Commingling Experimental Condition, Nataya Charoenvisal, Juthatip Keawcharoen, Donruethai Sreta, Siriporn Tantawet, Suphattra Jittimanee, Jirapat Arunorat, Korakrit Poonsuk, Alongkorn Amonsin, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Pandemic H1N1 2009 (pH1N1) virus is considered as a low pathogenic influenza virus, however, it rapidly spread among humans and was finally found in the swine population of 6 continents. Interspecies transmissions among different animal species are of interest. In this study, sentinel ducks were commingled with pH1N1 or pandemic H1N1 reassortment virus (rH1N1) inoculated pigs in separate groups. According to the results, both studied viruses were able to cross-species transmit to a few sentinel ducks with mild or no clinical signs. Viral shedding measured by a modified real time RT-PCR detection from the oropharyngeal and cloacal swabs were also …
Preference Testing As Environmental Enrichment Assessment For Laboratory Mice,
2013
Chulalongkorn University
Preference Testing As Environmental Enrichment Assessment For Laboratory Mice, Navakanit Sachanonta, Waridtha Sa-Ngeunreung, Somchai Sa-Ing-Kaew, Raywadee Butraporn
The Thai Journal of Veterinary Medicine
In the field of biomedical research, a wide variety of environmental enrichment items are available for laboratory mice to fulfill its physiological and behavioral needs which influence the outcome of animal experiments. However, to take a decision on the most suitable enrichment, researchers are likely to consider the cost of enrichment item more than animal preference and animal well-being. To develop a suitable environmental enrichment program for mice in Laboratory Animal Center, National Institute of Health (LAC-Thai NIH), we evaluated mouse preference for cages containing different types of nesting material and enrichment device. We carried out a simple preference test …
Mrna And Protein Expressions Of Prostaglandin E2 Receptors (Ep2 And Ep4), Cyclooxygenase-2 And Prostaglandin E Synthase In The Cervix Of Cyclic Bitches And Those With Pyometra,
2013
Chulalongkorn University
Mrna And Protein Expressions Of Prostaglandin E2 Receptors (Ep2 And Ep4), Cyclooxygenase-2 And Prostaglandin E Synthase In The Cervix Of Cyclic Bitches And Those With Pyometra, Pichanun Linharattanaruksa, Theerawat Swangchan-Uthai, Sayamon Srisuwatanasagul, Muhammad Khalid, Kaywalee Chatdarong
The Thai Journal of Veterinary Medicine
Prostaglandins play a vital role in regulation of cervical patency. Prostaglandin E2 (PGE2) synthesis is regulated by cyclooxygenase (COX) and prostaglandin E synthase (PGES). PGE2 acts through prostaglandin E receptors (EP), EP2 and EP4, to stimulate muscle relaxation. The aim of this study was to investigate mRNA expressions (EP2, EP4, COX-2, and PGES) and protein expressions (EP2, EP4, and COX-2) in the bitch cervix. Two groups of bitches, normal cyclic bitches and bitches with pyometra, were studied. Cyclic bitches were categorized into anestrus (n = 10), estrus (n = 7), and diestrus (n = 11), whereas bitches with pyometra were …
Mutations In Topoisomerase Genes And Expression Of Acrab Multidrug Efflux System In Fluoroquinolone-Resistant Salmonella Enterica From Pork And Patients,
2013
Chulalongkorn University
Mutations In Topoisomerase Genes And Expression Of Acrab Multidrug Efflux System In Fluoroquinolone-Resistant Salmonella Enterica From Pork And Patients, Wechsiri Wannaprasat, Rungtip Chuanchuen1
The Thai Journal of Veterinary Medicine
The aim of this study was to further examine the mechanisms of ciprofloxacin resistance in Salmonella enterica isolates from pork and patients. Twenty-four isolates including 18 ciprofloxacin- resistant isolates and six susceptible strains were used. Mutations in the quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and parE were examined. Expression of AcrAB-TolC efflux pump was quantitatively determined using qRT-PCR. While nucleotide modifications were found in gyrA, gyrB and parC, all mutations identified were novel. All 18 ciprofloxacin resistant isolates carried mutations in at least one target gene and all six isolates susceptible to ciprofloxacin did not carry mutations in …
