Preference Testing As Environmental Enrichment Assessment For Laboratory Mice,
2013
Chulalongkorn University
Preference Testing As Environmental Enrichment Assessment For Laboratory Mice, Navakanit Sachanonta, Waridtha Sa-Ngeunreung, Somchai Sa-Ing-Kaew, Raywadee Butraporn
The Thai Journal of Veterinary Medicine
In the field of biomedical research, a wide variety of environmental enrichment items are available for laboratory mice to fulfill its physiological and behavioral needs which influence the outcome of animal experiments. However, to take a decision on the most suitable enrichment, researchers are likely to consider the cost of enrichment item more than animal preference and animal well-being. To develop a suitable environmental enrichment program for mice in Laboratory Animal Center, National Institute of Health (LAC-Thai NIH), we evaluated mouse preference for cages containing different types of nesting material and enrichment device. We carried out a simple preference test …
Welfare Assessment Of Flight-Restrained Captive Birds: Effects Of Inhibition Of Locomotion,
2013
Chulalongkorn University
Welfare Assessment Of Flight-Restrained Captive Birds: Effects Of Inhibition Of Locomotion, Shawn Jen-Lung Peng, Fang-Chia Chang, Judy Sheng-Ting I, Andrew Chang-Young Fei
The Thai Journal of Veterinary Medicine
Morphological and physiological adaptations have allowed birds to utilize flying as part of the primary locomotion. However, birds in captivity are often deprived of this natural skill due to limited living space or wing amputation. This article aims to investigate the essentialness of flight and welfare assessment of flight restraint in captive birds, using animal-based measurement. Ten wild-caught great mynahs (Acridotheres grandis) were chosen as test subject and evaluated in three separate studies. In pathology study, bird wings were partially amputated to assess possible neuroma formation at the site of amputation. The birds involved in this study were evaluated on …
Mutations In Topoisomerase Genes And Expression Of Acrab Multidrug Efflux System In Fluoroquinolone-Resistant Salmonella Enterica From Pork And Patients,
2013
Chulalongkorn University
Mutations In Topoisomerase Genes And Expression Of Acrab Multidrug Efflux System In Fluoroquinolone-Resistant Salmonella Enterica From Pork And Patients, Wechsiri Wannaprasat, Rungtip Chuanchuen1
The Thai Journal of Veterinary Medicine
The aim of this study was to further examine the mechanisms of ciprofloxacin resistance in Salmonella enterica isolates from pork and patients. Twenty-four isolates including 18 ciprofloxacin- resistant isolates and six susceptible strains were used. Mutations in the quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and parE were examined. Expression of AcrAB-TolC efflux pump was quantitatively determined using qRT-PCR. While nucleotide modifications were found in gyrA, gyrB and parC, all mutations identified were novel. All 18 ciprofloxacin resistant isolates carried mutations in at least one target gene and all six isolates susceptible to ciprofloxacin did not carry mutations in …
Surgical Removal Of Foreign Bodies In The Gastrointestinal Tract Of Monocellate Cobra, Naja Kaouthia,
2013
Chulalongkorn University
Surgical Removal Of Foreign Bodies In The Gastrointestinal Tract Of Monocellate Cobra, Naja Kaouthia, Taksa Vasaruchapong, Lawan Chanhome
The Thai Journal of Veterinary Medicine
A wild-captured female monocellate cobra (Naja kaouthia) had abnormal posture and move with difficulty as consequences of swelling at the middle third of the body. The swelling area was firm on palpation with average size of 10x4 cm. The shape of the swelling area was similar to a small bottle which was obviously recognized on the dorsal recumbency. Therefore, the foreign body obstruction in gastrointestinal tract was diagnosed without radiographic examination. Digital manipulation was performed, but could not move the foreign bodies. Therefore, gastrotomy was considered. The foreign bodies were a plastic bottle and a piece of cloth which were …
Comparison Of Repetitive Sequence-Based Polymerase Chain Reaction (Rep-Pcr) And Pulsed-Field Gel Electrophoresis (Pfge) For Genetic Characterization Of Arcobacter Spp.,
2013
Chulalongkorn University
Comparison Of Repetitive Sequence-Based Polymerase Chain Reaction (Rep-Pcr) And Pulsed-Field Gel Electrophoresis (Pfge) For Genetic Characterization Of Arcobacter Spp., Panvipa Phasipol, Nipa Chokesajjawatee, Taradon Luangtongkum
The Thai Journal of Veterinary Medicine
Arcobacter has been associated with foodborne illness in humans. Recently, this organism has been receiving more attention as a pathogen of public health concern. The contamination of Arcobacter is frequently observed in foods of animal origin especially poultry products; however, the source of contamination as well as the molecular epidemiology of Arcobacter is not clearly understood. In the present study, we compared the use of repetitive sequence-based polymerase chain reaction (rep-PCR) and pulsed-field gel electrophoresis (PFGE) for genetic characterization of Arcobacter. Thirty Arcobacter butzleri isolates from retail chicken carcasses and 3 Arcobacter reference strains were typed with rep-PCR and PFGE. …
Susceptibility Of Clostridium Difficile Isolated From Healthy Captive Asian Elephants To Metronidazole And Vancomycin,
2013
Chulalongkorn University
Susceptibility Of Clostridium Difficile Isolated From Healthy Captive Asian Elephants To Metronidazole And Vancomycin, Nattawooti Sthitmatee, Thanya Warinrak, Waroot Wongkalasin
The Thai Journal of Veterinary Medicine
Susceptibility to metronidazole and vancomycin, drugs of choice for Clostridium difficile infection, of 15 C. difficile isolates from 6 healthy Asian elephants was determined. All of the isolates belonged to only 1 ribotype pattern and carried both toxin A and B genes. The Minimal inhibitory concentration range of metronidazole and vancomycin, drugs of choice for treatment of C. difficile infection, was 0.125-4.0 μg/ml and 0.125-2.0 μg/ml, respectively. Moreover, MIC50 and MIC90 for metronidazole were 0.75 and 1.5 μg/ml while vancomycin was 1.0 and 2.0 μg/ml. There was no evidence of resistance to these antimicrobials. These results might be a preliminary …
Ultrasound Diagnosis,
2013
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Mrna And Protein Expressions Of Prostaglandin E2 Receptors (Ep2 And Ep4), Cyclooxygenase-2 And Prostaglandin E Synthase In The Cervix Of Cyclic Bitches And Those With Pyometra,
2013
Chulalongkorn University
Mrna And Protein Expressions Of Prostaglandin E2 Receptors (Ep2 And Ep4), Cyclooxygenase-2 And Prostaglandin E Synthase In The Cervix Of Cyclic Bitches And Those With Pyometra, Pichanun Linharattanaruksa, Theerawat Swangchan-Uthai, Sayamon Srisuwatanasagul, Muhammad Khalid, Kaywalee Chatdarong
The Thai Journal of Veterinary Medicine
Prostaglandins play a vital role in regulation of cervical patency. Prostaglandin E2 (PGE2) synthesis is regulated by cyclooxygenase (COX) and prostaglandin E synthase (PGES). PGE2 acts through prostaglandin E receptors (EP), EP2 and EP4, to stimulate muscle relaxation. The aim of this study was to investigate mRNA expressions (EP2, EP4, COX-2, and PGES) and protein expressions (EP2, EP4, and COX-2) in the bitch cervix. Two groups of bitches, normal cyclic bitches and bitches with pyometra, were studied. Cyclic bitches were categorized into anestrus (n = 10), estrus (n = 7), and diestrus (n = 11), whereas bitches with pyometra were …
Coccidiosis In A Greater Slow Loris (Nycticebus Coucang),
2013
Chulalongkorn University
Coccidiosis In A Greater Slow Loris (Nycticebus Coucang), Wijit Banlunara, Sawang Kesdangsakonwut, Piyaporn Kongmekee, Angkana Sommanustweechai
The Thai Journal of Veterinary Medicine
Since Eimeria pachylepyron was firstly described in slow loris (Nycticebus coucang) in 1972, there has not been any reports of the disease throughout the world. An adult male 0.8 kg bw greater slow loris (Nycticebus coucang) had diarrhea, emaciation and dead. Severe necro-hemorrhagic jejunitis with the presence of coccidian protozoa and secondary bacterial septicemia were the causes of death. Endogenous stages of E. pachylepyron were detected exclusively in the jejunal enterocytes especially in the crypt of Lieberkühn. The typical round unsporulated oocysts with homogeneous eosinophilic thick wall were closely related to E. pachylepyron. This is the first report to confirm …
Ophthalmology Snapshot,
2013
Chulalongkorn University
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
Indices Of Myocardial Contractility,
2013
Chulalongkorn University
Indices Of Myocardial Contractility, Anusak Kijtawornrat
The Thai Journal of Veterinary Medicine
Myocardial contractility is defined as the velocity of cycling of heavy meromyosin cross-bridges. Contractility may be defined also as the velocity of myocardial fiber shortening or change in ventricular dimension during contraction when the fiber shortens or the ventricle contracts against no load. In the clinic; however, myocardial contractility may be estimated by some parameters of systolic function adjusted for the degree of stretch on the fibers (the preload) just before contraction begins and the hindrance to ejection of blood (the afterload). Excitation-contraction coupling is a physicochemical process during which electrophysiological changes (initiated by depolarization of the cell) translate to …
Expression Of Oestrogen Receptor Alpha And Progesterone Receptor In Sow Oviducts After Different Artificial Insemination,
2013
Chulalongkorn University
Expression Of Oestrogen Receptor Alpha And Progesterone Receptor In Sow Oviducts After Different Artificial Insemination, Sayamon Srisuwatanasagul, Padet Tummaruk, Annop Kunavongkrit
The Thai Journal of Veterinary Medicine
In pig production, different insemination methods which allow deposition of sperm in the uterine body (intrauterine insemination, IUI) and uterine horn (deep intrauterine insemination) have been applied in order to reduce number of spermatozoa. Earlier studies reported that steroid hormones influenced the transportation of spermatozoa, ovum and embryos in the sow reproductive tract which was related to the presence of their specific receptors; oestrogen receptor alpha (ERα) and progesterone receptor (PR). Therefore, the present study aims to evaluate the immunolocalization of ERα and PR in different parts of sow oviducts, after different artificial insemination methods. Twelve sows were divided into …
Effects Of Myogenic Factor 5 (Myf5) Gene On Carcass And Meat Quality Of Mong Cai Pigs,
2013
Chulalongkorn University
Effects Of Myogenic Factor 5 (Myf5) Gene On Carcass And Meat Quality Of Mong Cai Pigs, Thi Kim Khang Nguyen, Trong Ngu Nguyen
The Thai Journal of Veterinary Medicine
The aim of this study was to analyze the impact of Myogenic Factor 5 gene (MYF5) on carcass and meat quality of Mong Cai pigs. PCR-RFLP was applied to genotype 100 animals at three loci defined as MYF5/Hin1II, MYF5/HaeIII and MYF5/MspI. Results showed that MC pigs had two genotypes at each locus namely CD and DD,EF and EE, and GH and HH for the MYF5/Hin1II, MYF5/HaeIII and MYF5/MspI loci, respectively. The frequencies of DD, EE and HH were predominant (0.78-0.94) in the population. In addition, association analysis of three loci revealed that animals carrying CD genotype provided higher dressing percentage …
Development And Application Of Reverse Transcription Loop-Mediated Isothermal Amplification (Rt-Lamp) For Feline Coronavirus Detection,
2013
Chulalongkorn University
Development And Application Of Reverse Transcription Loop-Mediated Isothermal Amplification (Rt-Lamp) For Feline Coronavirus Detection, Somporn Techangamsuwan, Araya Radtanakatikanon, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Mutated feline coronavirus (FCoV) causes a highly fatal disease in cats, named feline infectious peritonitis (FIP). Common FIP clinical signs represent an accumulation of fluid in the thorax and/or abdomen. Screening tests currently rely on fluid analysis with cytologic examination to differentiate cellular components. Accordingly, accurate ante-mortem diagnosis is prerequisite for effective treatment strategies. This study focused on developing a rapid diagnostic tool for FCoV by using reverse transcription loop-mediated isothermal amplification (RT-LAMP). Clinical samples composed of bodily fluids, plasma and feces were obtained from FIP-suspected cats (n = 63), apparent healthy cats living with (n = 12) and without …
Effect Of Polysaccharide Gel Extracted From Durio Zibethinus Rind On Immune Responses, Bacteria Counts And Cholesterol Quantities In Chickens,
2013
Chulalongkorn University
Effect Of Polysaccharide Gel Extracted From Durio Zibethinus Rind On Immune Responses, Bacteria Counts And Cholesterol Quantities In Chickens, Nustha Kitprathaung, Nattaya Ngamrojanavanich, Piyarat Chansiripornchai, Sununta Pongsamart, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
Polysaccharide gel (PG) from the rind of durian (Durio zibethinus Murr.) is a soluble powder that gives good results as an excipient in pharmacological and food preparation. The aim of the present study was to evaluate the effects of PG as a feed-supplement diet on body weight gain, immune stimulation, total bacteria and Salmonella in feces, and cholesterol levels in broilers. Eighty, one-day-old broiler chicks were divided into 4 groups. Three experimental groups were fed a commercial diet coated with PG 1, 2 and 3 g/100 g, respectively, and the control group was fed a commercial diet without PG. The …
Production Of Black Goat Using Laparoscopic Artificial Insemination And Embryo Transfer,
2013
Chulalongkorn University
Production Of Black Goat Using Laparoscopic Artificial Insemination And Embryo Transfer, Nitira Anakkul, Junpen Suwimonteerabutr, Theerawat Tharasanit, Saritvich Panyaboriban, Sarawanee Khunmanee, Natthakarn Thanomsuksinchai, Mongkol Techakumphu1
The Thai Journal of Veterinary Medicine
Laparoscopic artificial insemination (LAI) and embryo transfer (ET) were used to produce cross-bred blackcolored goat. In Experiment 1, LAI with frozen-thawed semen of black buck (Australian Melaan) was performed in 75% Saanen cross-bred does (white color, n = 70). The total numbers of 68 kids were born from 50 does. The skin colors of kids born were black (10.29%), white (39.71%) and other colors (50%). In Experiment 2, two cross-breeding programs were tested including program I: frozen semen of Australian Melaan inseminated to Black Bengal female (n = 7) and program II: frozen semen of Black Bengal inseminated to 50% …
Prevalence Of Salmonella Enterica, Escherichia Coli And Staphylococcus Aureus In Raw Meat In Thai Self-Service Style Restaurants In Khon Kaen Municipality,
2013
Chulalongkorn University
Prevalence Of Salmonella Enterica, Escherichia Coli And Staphylococcus Aureus In Raw Meat In Thai Self-Service Style Restaurants In Khon Kaen Municipality, Sunpetch Angkititrakul, Arunee Polpakdee, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
One hundred and fifty eight raw meat samples were collected from 33 Thai self-service style restaurants in Khon Kaen municipality during July-October 2011. The fresh meat included pork, beef and chicken and was provided for self-grilling or boiling. All the samples were examined for contamination of three major foodborne pathogens including Salmonella enterica, Escherichia coli and Staphylococcus aureus using standard protocol. High prevalence of S. enterica (44%), E. coli (60%) and S. aureus (25%) was observed. Seventy-seven percent of the samples were positive for at least one of the three pathogens, while only 8% were found to carry all three …
Histological Characteristics Of Auditory Tube Diverticulum Of Domestic Donkey (Equus Asinus),
2013
Chulalongkorn University
Histological Characteristics Of Auditory Tube Diverticulum Of Domestic Donkey (Equus Asinus), Seyed Morteza Ghazi, Behzad Mobini
The Thai Journal of Veterinary Medicine
The purpose of this study was to investigate histological characteristics of auditory tube diverticulum in domestic donkeys (Equus asinus) using conventional histological and histochemical methods. Specimens were collected from twenty regions (five different locations of each compartment of auditory diverticulum). The mucosa consisted of pseudostratified columnar epithelium with brush-like cilia and forms of some non-isometric folds. The few goblet cells, which were irregularly distributed over the whole luminal surface, reacted strongly with Alcian blue pH 2.5, whereas neutral mucosubstances were not observed. The lamina muscularis mucosa was absent. The lamina propria-submucosa contained all connective tissue fibers, tubulo-acinar glands, parasympathetic ganglia, …
Antiparasitic Efficacy Of 10% W/V Fipronil Spot-On (Fiproline Spot-On) Against Experimental Tick (Rhipicephalus Sanguineus) Infestations On Dogs,
2013
Chulalongkorn University
Antiparasitic Efficacy Of 10% W/V Fipronil Spot-On (Fiproline Spot-On) Against Experimental Tick (Rhipicephalus Sanguineus) Infestations On Dogs, Sonthaya Tiawsirisup, Kanokwan Thiansirikhun, Kanoksak Thanadumkerng, Nuttika Pastarapatee, Nunaree Trirattananuwong, Wilai Rattanatayarom
The Thai Journal of Veterinary Medicine
This study was conducted to investigate the efficacy of 10% w/v fipronil spot-on for treatment and prevention of Rhipicephalus sanguineus infestations on dogs. Twelve Beagles were randomly separated into two groups which were treatment and control group (6 dogs/group). The treatment group received 10% fipronil spot-on at the dosage of 6.7 mg/kg on day 0. A group of 60 Rhipicephalus sanguineus was released to feed on each dog on day -7, -2, 7, 14, 21, and 28. The ticks were counted and removed on days-4, 3, 10, 17, 24, and 31. Mean number of tick infestations in the control group …
Different Influence Of Intracellular Glutamine Depletion On Glutamate Uptake Mediated By Amino Acid Transport Systems; Eaats And X-C, In Placental (Bewo) Cells,
2013
Chulalongkorn University
Different Influence Of Intracellular Glutamine Depletion On Glutamate Uptake Mediated By Amino Acid Transport Systems; Eaats And X-C, In Placental (Bewo) Cells, Boonrit Thongsong
The Thai Journal of Veterinary Medicine
Glutamate transporters are responsible for active transport of glutamate over the cell membrane and play an important role in the glutamate-glutamine cycle. The purpose of the present study was to investigate the influence of intracellular glutamine depletion on glutamate uptake mediated by amino acid transport systems; EAATs and X-c in placental choriocarcinoma (BeWo) cells as a model of human trophoblasts. Intracellular glutamine was depleted by culturing the cells in regular medium without glutamine and by treating with an inhibitor of glutamine synthetase (methionine sulfoximine; MSX), for 16 hours. The uptake of glutamate was measured by the use of appropriate substrates …
