Veterinary Medicine Commons^™

The Effects Of Propofol And A Diazepam/Alfentanil Combination In Dogs Aged 10 Years And Above On Heart Rate, Respiratory Rate, Pulse Oximetry Data, Intraocular Pressure, And Body Temperature, Özlem Güzel, Di̇lek Olğun Erdi̇kmen, Esma Yildar, Asli Eki̇ci̇, Murat Şaroğlu, Bülent Eki̇z

Turkish Journal of Veterinary & Animal Sciences

In older patients, it is important to minimise anaesthesia-related deaths and complications. In this study, 2 separate anaesthesia inductions were evaluated with respect to their effects on heart rate, respiratory rate, pulse oximetry data, intraocular pressure, and body temperature. A total of 22 dogs were evaluated and 2 separate anaesthesia groups were formed. Propofol (6 mg/kg) was administered to group G1 via a slow intravenous (IV) injection. In group G2, diazepam (0.5 mg/kg) was first administered via an IV injection, followed immediately by alfentanil (40 µg/kg), administered via a slow IV route. Each parameter was recorded prior to anaesthesia (T_0), …

Factors Affecting Colostrum Quality Of Ewes And Immunostimulation, Özge Yilmaz, Güven Kaşikçi

Turkish Journal of Veterinary & Animal Sciences

Maternal antibodies play a major role in the development of the immune system of a young animal. Ideally, the transmission of maternal immunity to the foetus occurs in the uterus. However, in ruminants, as there is no maternal antibody transfer from the mother to her young by way of the uterus, the antibodies present in the colostrum are a vital necessity for newborn ruminants. After birth, an unhealthy neonatal period for the young animal and an unhealthy puerperal period for the mother can cause significant financial losses. In animal husbandry, due to the budget allocated for the treatment of diseases …

Glycohistochemistry Of The Lateral Tympanic Membrane In The Syrinx Of The Denizli Cock, Nazan Keski̇n, Pinar İli̇

Turkish Journal of Veterinary & Animal Sciences

The Denizli cock is a special race that has harmonious long crowing. In this study, the syrinx of the Denizli cock was studied by using glycohistochemical analyses. Paraffin sections were stained with Alcian blue (AB) at pH 1 and pH 2.5 for demonstration of sulfated and carboxylated carbohydrates, respectively. At AB-pH 1, the lateral tympanic membrane (LTM) and also the medial tympanic membrane (MTM) exhibited a weak reactivity, whereas they were stained intensely at AB-pH 2.5. The frozen sections were incubated with 5 digoxigenin-labeled lectins for lectin histochemistry: peanut agglutinin, Maackia amurensis leucoagglutinin, Galanthus nivalis agglutinin, Datura stramonium agglutinin, and …

The Effect Of Electron Beam Irradiation On Β-Glucan Content, X-Ray Diffraction Of Starch, Protein Subunit Patterns, And In Vivo Digestibility Of Barley Grain In Cockerels, Parvin Shawrang, Ali Asghar Sadeghi, Behnam Ghorbani

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to evaluate the effect of electron beam radiation on the chemical composition, the \beta-glucan content, the water extract viscosity, the crystallinity of starch, the patterns of protein subunits, and the in vivo digestibility of barley grain. Barley samples were irradiated at doses of 10, 15, 20, 25, and 30 kGy under the electron beam. In a completely randomized design, cockerels were fed raw or irradiated samples to determine the digestibility of crude protein and gross energy. Irradiation had no effect on the total contents of protein, starch, and \beta-glucan. At doses of 10, 15, 20, …

Acute Toxicity Of Diazinon And Its Effects On Hematological Parameters In The Indian Carp, Cirrhinus Mrigala (Hamilton), Abdul Rauf, Naeemuddin Arain

Turkish Journal of Veterinary & Animal Sciences

Diazinon is a commonly used pesticide and its existence in the fresh water reservoirs of Pakistan has been reported by some authors. A semistatic experiment was conducted to find out the acute toxicity of diazinon as an aquatic pollutant on some hematological parameters of Indian carp (Cirrhinus mrigala). The 1, 24, 48, 72, and 96 h LC50 values of diazinon for Indian carp were estimated as 17.32 mg/L, 15.39 mg/L, 12.64 mg/L, 10.98 mg/L, and 8.15 mg/L respectively. A significant increase in the number of dead fish was observed as the concentration of diazinon and exposure time increased (P < 0.05). The results after acute exposure to 8.15 mg/L of diazinon for 96 h showed that compared to the control fish, the total erythrocytes, leukocytes, hemoglobin, and hematocrit values decreased significantly (P < 0.05) in exposed fish. The mean corpuscular volume and mean corpuscular hemoglobin increased significantly (P < 0.05), while the mean corpuscular hemoglobin concentration of both groups remained unchanged during the study. In conclusion, acute exposure to LC50 values of diazinon induced hematological alterations in Indian carp and offers a tool to evaluate toxicity derived alterations.

Prevalence And Antibiotic Resistance Of Vancomycin-Resistant Enterococci In Animal Originated Foods, Fi̇gen Çeti̇nkaya, Tülay Elal Muş, Gül Ece Soyutemi̇z, Recep Çibik

Turkish Journal of Veterinary & Animal Sciences

A total of 270 animal-originated foods purchased from markets, including milk and dairy products, fresh and processed meat products, and poultry and poultry products, were analysed to determine the prevalence of vancomycin-resistant enterococci (VRE). VRE were isolated from 12 out of 270 samples (4.4%): 6 (6%) from fresh meat and meat products and 6 (8.6%) from poultry and poultry products. VRE strains were identified as Enterococcus faecium (6 strains), E. avium (4 strains), E. durans (1 strain) and E. gallinarum (1 strain). Among these strains, 11 had high levels of vancomycin resistance (2 E. faecium strains had minimum inhibitory concentrations …

Rhodotorula Minuta Fungemia In A Ewe Lamb, C. G. Chitko-Mckown, K. A. Leymaster, M. P. Heaton, D. D. Griffin, J. K. Veatch, S. A. Jones, M. L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary Diagnostic Laboratory at Colorado State University for fungal diagnosis and was found negative for Aspergillus, Blastomyces, Coccidioidomycosis and Histoplasmosis. DNA was isolated from fungi collected from tissue culture supernatants and used in a set of pan-fungal PCR assays with …

Interferon Inducible Protein Ifi35 Negatively Regulates Rig-I Antiviral Signaling And Supports Vesicular Stomatitis Virus Replication, Anshuman Das, Phat X. Dinh, Debasis Panda, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

In a genome-wide siRNA screen, we recently identified the interferon (IFN) inducible protein 35 (IFI35, also known as IFP35) as a factor required for VSV infection. Studies reported here were conducted to further understand the role and requirement of IFI35 in VSV infection. Consistent with the siRNA screening data, we found that depletion of IFI35 led to reduced VSV replication at the level of viral gene expression. Although no direct interaction of IFI35 with the viral replication machinery was observed, we found that IFI35 negatively regulated the host innate immune response and rescued poly(I:C)-induced inhibition of VSV replication. Promoter-driven reporter …

Sequence-Optimized And Targeted Double-Stranded Rna As A Therapeutic Antiviral Treatment Against Infectious Myonecrosis Virus In Litopenaeus Vannamei, J. Dustin Loy, Duan S. Loy, Mark A. Mogler, Bruce Janke, Kurt Kamrud, D. L. Hank Harris, Lyric C. Bartholomay

School of Veterinary and Biomedical Sciences: Faculty Publications

Infectious myonecrosis virus (IMNV) is a significant and emerging pathogen that has a tremendous impact on the culture of the Pacific white shrimp Litopenaeus vannamei. IMNV first emerged in Brazil in 2002 and subsequently spread to Indonesia, causing large economic losses in both countries. No existing therapeutic treatments or effective interventions currently exist for IMNV. RNA interference (RNAi) is an effective technique for preventing viral disease in shrimp. Here, we describe the efficacy of a double-stranded RNA (dsRNA) applied as an antiviral therapeutic following virus challenge. The antiviral molecule is an optimized dsRNA construct that targets an IMNV sequence …

Bovine Herpes Virus 1 (Bhv-1) And Herpes Simplex Virus Type 1 (Hsv-1) Promote Survival Of Latently Infected Sensory Neurons, In Part By Inhibiting Apoptosis, Clinton Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

α-Herpesvirinae subfamily members, including herpes simplex virus type 1 (HSV-1) and bovine herpes virus 1 (BHV-1), initiate infection in mucosal surfaces. BHV-1 and HSV-1 enter sensory neurons by cell-cell spread where a burst of viral gene expression occurs. When compared to non-neuronal cells, viral gene expression is quickly extinguished in sensory neurons resulting in neuronal survival and latency. The HSV-1 latency associated transcript (LAT), which is abundantly expressed in latently infected neurons, inhibits apoptosis, viral transcription, and productive infection, and directly or indirectly enhances reactivation from latency in small animal models. Three anti-apoptosis genes can be substituted for LAT, which …

Gastroscopic Evaluation Of Gastric Ulcer Syndrome In Sport Horses With Poor Performance, Mehmet Alper Çeti̇nkaya, Alper Demi̇rutku, Mahi̇r Kaya

Turkish Journal of Veterinary & Animal Sciences

Gastric ulceration is common in performance horses. More than 90% of thoroughbred horses in the training season and approximately 60% of racing horses from other disciplines have gastric ulcers. Excessive secretion of gastric acid, impairment of gastric mucosal blood flow, weakening of prostaglandin E or the gastric mucus/bicarbonate layer, and gastric emptying disorders are important factors in the onset of ulceration. In this study, 12 horses with poor performance were evaluated using gastroscopy following clinical examinations. Gastroscopy revealed ulceration in 5 horses and ulcerative lesions in 7. A daily dose of 4 mg/kg omeprazole was administered orally for 4 weeks …

Optimization Of Feline Adipose-Derived Multipotent Stromal Cell Isolation And Canine Cranial Cruciate Ligament Regeneration With Intra-Articular Adipose-Derived Multipotent Stromal Cells, Nan Zhang

LSU Doctoral Dissertations

Adult multipotent stromal cells (MSCs) have become popular within veterinary sciences for direct administration and tissue regeneration due to their differentiation, trophic and immunosuppressive properties. However, current isolation and expansion techniques provide a heterogeneous population with other types of cells, which may affect the efficiency and efficacy of treatments. The progenitor properties and function need to be further evaluated in vitro prior to in vivo application. The first part of this dissertation was to identify the optimal MSC source for general canine orthopedic applications such as intra-articular injection and joint tissue regeneration. Based on side-by-side comparisons of cell doublings, colony …

Equine Protozoal Myeloencephalitis: Investigation Of Genetic Susceptibility And Assessment Of An Equine Infection Method, Breanna M. Gaubatz

Theses and Dissertations--Veterinary Science

Equine protozoal myeloencephalitis (EPM) is a progressive neurological disease of horses caused by Sarcocystis neurona. Two projects were conducted to identify factors involved in the development of EPM. The first study explored a possible genetic susceptibility to EPM by attempting a genome-wide association study (GWAS) on formalin-fixed, paraffin-embedded (FFPE) tissue from 24 definitively-positive EPM horses. DNA extracted from tissues older than 14 months was inadequate for SNP analysis on the Illumina Equine SNP50 BeadChip probably due to degradation and formalin cross-linking. Results were inconclusive as analysis was not possible with the small sample set. The second study evaluated an …

Determination Of Farm-Specific Lawsonia Intracellularis Seroprevalence In Central Kentucky Thoroughbreds And The Identification Of Factors Contributing To Equine Proliferative Enteropathy, Allen E. Page

Theses and Dissertations--Veterinary Science

Lawsonia intracellularis and the disease it causes in horses, equine proliferative enteropathy (EPE), is an emerging pathogen of increasing importance to the horse industry from both an economic and welfare standpoint. Long recognized as an economically important disease of swine, the hallmark of EPE is a protein-losing enteropathy, where affected horses suffer weight loss and some ultimately succumb to the disease despite aggressive treatment. There are currently no known EPE preventative measures and the epidemiology of the disease remains poorly defined. While EPE is a sporadic disease affecting less than 25% of exposed horses, some farms experience clinical cases year …

Chondrodysplasia-Like Dwarfism In The Miniature Horse, John E. Eberth

Theses and Dissertations--Veterinary Science

Dwarfism is considered one of the most recognized congenital defects of animals and humans and can be hereditary or sporadic in cause and expression. There are two general morphologic categories within this vastly diverse disease. These categories are disproportionate and proportionate dwarfism and within each of these there are numerous phenotypes which have been extensively described in humans, and to a lesser extent in dogs, cattle, mice, chickens, and other domestic species. Ponies and Miniature horses largely differ from full size horses only by their stature. Ponies are often defined as those whose height is not greater than 14.2 hands; …

Signal Transduction And Rickettsial Infection Of Tick Cells, Natthida Petchampai

LSU Doctoral Dissertations

Spotted fever group (SFG) Rickettsia are obligate intracellular bacteria that are carried by ticks. One such tick, Dermacentor variabilis is a vector for the etiologic agent of Rocky Mountain spotted fever, R. rickettsii. These ticks also carry a non-pathogenic R. montanensis, the agent used in this study. Interestingly, field data collected from infected D. variabilis throughout the United States revealed that the majority of Rickettsia in ticks are non-pathogenic species such as R. montanensis. Although ticks serve as both vector and reservoir hosts for SFG Rickettsia, many questions regarding tick-Rickettsia interaction remain unresolved. Therefore, the overall goal of this research …

Computed Tomographic Assessment Of Canine Arytenoid Lateralization, James Richard Wignall

LSU Master's Theses

Unilateral arytenoid lateralization is a commonly performed surgical treatment for laryngeal paralysis in dogs. It involves fixing the moveable arytenoid cartilage to the thyroid (TAL) or cricoid (CAL) cartilage or both (CTAL). This increases the area of the rima glottidis (RGA), to allow reduced airway pressure and laryngeal resistance in vitro and ameliorates clinical signs in vivo. It may also increase the patient’s predisposition for aspiration pneumonia, which occurs in around 20% of clinical patients. No surgical technique has been correlated with clinical outcome or risk of aspiration pneumonia. Objective analysis of the effects of surgery on the three dimensional …

Copper Oxide Wire Particles Used To Control Haemonchus Infections : Efficacy In Giraffe (Giraffa Camelopardalis) At Busch Gardens Tampa And Potential Mechanism Of Action, Allyson Kinney Moscona

LSU Master's Theses

Gastrointestinal nematode (GIN) infections affect production systems and exotic hoofstock in zoos, particularly giraffe (Giraffa camelopardalis). Anthelmintic resistance (AR) prevalence is increasing in production systems and zoos. To combat the AR that compounds GIN problems, alternative control methods are used. One such alternative is copper oxide wire particles (COWP), which control the abomasal nematode Haemonchus contortus. COWP was given to seven adult giraffe at Busch Gardens Tampa, at descending dosages: 25 g, 12.5 g, 6.3 g. Treatment administration time was determined by fecal egg count (FEC) being above 600 eggs per gram (EPG), and therefore varied with each animal. FEC …

Growing South Dakota (Winter 2013), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2012 South Dakota Agricultural Station Annual Report

CONTENTS:
[Page] 1 From The Dean
[Page] 2 Commitment To Collaboration: Working Together Adds Efficiency To Budgets: Effectiveness To Solutions
[Page] 7 Special Pull-Out Section: South Dakota Agricultural Experiment Station Annual Report: Highlighting Research Projects On Beef; Wheat; And Soybean Meal For Aquaculture
[Page] 11 Water Watchers: The South Dakota Water Resources Institute Provides Research, Education & Outreach
[Page] 12 Winter College News
[Page] 14 SDSU Extension: One Year Later: Reorganization Prompted Restructuring – And Renewed Commitment
[Page] 16 Bullseye!: South Dakota’s 4-H Shooting Sports Program Sees Continued Growth …

Effects Of Feeding Microbial Feed Additives On Growth Performance And Carcass Traits Of Steers Fed Steam-Flaked Corn-Based Diets With Wet Distillers Grains Plus Solubles, Matt K. Luebbe, Karla H. Jenkins, Stephanie A. Furman Furman, Kelly K. Kreikemeier

Nebraska Beef Cattle Reports

An experiment was conducted to determine the effects of feeding two commercially available direct-fed microbials (DFM) on finishing steer performance fed steam-flaked corn based diets. Dietary treatments included a control diet without DFM, and two commercially available products (10-G and Bovamine). No significant differences were observed among treatments for animal performance or carcass characteristics. However, numeric advantages were observed for ADG and feed efficiency when cattle were fed a DFM.