Safety And Toxicity Evaluation Of Bronopol In Striped Catfish (Pangasianodon Hypophthalmus),
2013
Chulalongkorn University
Safety And Toxicity Evaluation Of Bronopol In Striped Catfish (Pangasianodon Hypophthalmus), Patharapol Piamsomboon, Mintra Lukkana, Janenuj Wongtavatchai
The Thai Journal of Veterinary Medicine
The study was performed to evaluate the safety and toxicity of bronopol in striped catfish Pangasianodon hypophthalmus. Bronopol product is licensed in Thailand and Vietnam for control of aquatic fungi and ciliated protozoa in scaleless fish at the daily dosage of 10 mg/l, 2-hour static immersion, for 5 consecutive days. Safe concentrations were evaluated in fish exposed to bronopol at the therapeutic dosage (10 mg/l, 2 hours, 5 consecutive days) and 2 and 3 times overdose (20 and 30 mg/l, 2 hours, 5 consecutive days). Fish treated with 10-30 mg/l bronopol for 2 hours daily for 5 consecutive days appeared …
Molecular Characterization Of Chicken Infectious Anemia Virus Outbreaks During 2008-2011 In Thailand,
2013
Chulalongkorn University
Molecular Characterization Of Chicken Infectious Anemia Virus Outbreaks During 2008-2011 In Thailand, Wisanu Wanasawaeng, Jirayu Buatong, Supawadee Chaichote, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
The present study aimed to characterize chicken infectious anemia virus isolated from various outbreaks in Thailand during 2008-2011, and compare them with other strains from the GenBank database using molecular and bioinformatic techniques. For this purpose, thirteen samples were collected from eight commercial farms affected by chicken infectious anemia outbreak. All were positive by PCR and grouped in 2 clusters with phylogenetic analysis based on nucleotide sequencing, consistent with amino acid sequence analysis of the VP1 gene in the hypervariable region. All of the thirteen strains were closely related to Chinese and Japanese strains with a high percentage of sequence …
Testicular Atrophy And Its Related Changes In Culled Boars: A Pathological Investigation,
2013
Chulalongkorn University
Testicular Atrophy And Its Related Changes In Culled Boars: A Pathological Investigation, Komkrich Teankum, Padet Tummaruk, Sawang Kesdangsakonwut, Thiti Antarasena, Sithichok Lacharoj, Jinda Singlor, Annop Kunavongkrit, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Testicular atrophy is a major cause of culling in boars. Pathogenesis of this change is poorly understood. Therefore, we aimed to investigate pathological changes of the genital tracts of culled boars with testicular atrophy. Twenty-eight genital organs of culled boars collected from slaughter houses in Thailand were pathologically examined. According to testicular weight, the organs were allocated into 4 groups: severe atrophy (n = 12), moderate atrophy (n = 4), mild atrophy (n = 4), and normal control (n = 8). In the severe atrophic group, the mean weight of atrophic testes (175.1 ± 51 g) was lower than those …
Determination Of Antimicrobial Susceptibility, Antimicrobial Resistance Genes And In Vivo Testing Of Antimicrobial Susceptibility Of Avibacterium Paragallinarum,
2013
Chulalongkorn University
Determination Of Antimicrobial Susceptibility, Antimicrobial Resistance Genes And In Vivo Testing Of Antimicrobial Susceptibility Of Avibacterium Paragallinarum, Tippayaporn Noonkhokhetkong, Kridda Chukiatsiri, Jiroj Sasipreeyajan, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
Avibacterium paragallinarum is the causative agent of infectious coryza, an acute upper respiratory disease in chickens. The aim of this study was to characterize the antimicrobial susceptibility, resistance genes and the in vivo testing of antimicrobial susceptibility in chickens. Eighteen A. paragallinarum isolated in Thailand were tested for antimicrobial susceptibility by broth microdilution method. A high prevalence of resistance MIC pattern to oxytetracycline, doxycycline, streptomycin, ciprofloxacin, erythromycin and sulfamethoxazole-trimethoprim was found. Thirteen isolates showed multiple antimicrobial drug resistance (66.7%). Resistance genes of tet(A), tet(B), tet(M), erm(A), erm(B), blaROB-1 and sul2 were found and the resistance genes of tet(A), tet(B) and …
Screening And Characterization Of Lactic Acid Bacteria From Animal Faeces For Probiotic Properties,
2013
Chulalongkorn University
Screening And Characterization Of Lactic Acid Bacteria From Animal Faeces For Probiotic Properties, Tanatip Thamacharoensuk, Nuttha Thongchul, Malai Taweechotipatr, Vasana Tolieng, Kentaro Kodama, Somboon Tanasupawat
The Thai Journal of Veterinary Medicine
Twenty-two bacterial isolates from wild elephant faeces and 4 isolates from buffalo faeces in Thailand were investigated for probiotic properties and their lactic acid production. On the basis of their phenotypic characteristic clustering and 16S rRNA gene sequence analysis, the isolates were divided into seven groups. Group I (10 isolates) was identified as Enterococcus hirae, group II (4 isolates) as E. avium, group III (3 isolates) as Pediococcus pentosaceus , IV (3 isoaltes) as P. acidilactici, group V (3 isolates) as Lactobacillus pentosus, group VI (1 isolate) as Lactococcus garvieae and group VII (2 isolates) as Weissella paramesenteroides. All of …
Pathogenicity And Transmission Of Thai Hpai-H5n1 In Japanese Quails,
2013
Chulalongkorn University
Pathogenicity And Transmission Of Thai Hpai-H5n1 In Japanese Quails, Thawat Lekdumrongsak, Wijit Banlunara, Rachod Tantilertcharoen, Nutthawan Nonthabenjawan, Komkiew Pinpimai, Jiroj Sasipreeyajan, Alongkorn Amonsin
The Thai Journal of Veterinary Medicine
Pathogenicity and transmission of Highly Pathogenic Avian Influenza subtype H5N1 viruses (HPAI-H5N1) depend on the strains and infection doses of the viruses. In this study, experimental infection was performed to determine pathogenicity and transmission of the Thai HPAI-H5N1 in Japanese quails. One hundred and seven quails were divided into 4 groups, experimental groups 1-3 (n = 32 per group) and negative control group (n = 11). At the age of 9 weeks, 16 quails each from groups 1-3 were inoculated with 107, 105 and 103 EID50 of Thai HPAI-H5N1, A/chicken/Thailand/CUK2/04(CU-K2) virus. Twenty-four hours later, 16 quails were added as contact …
Critical Factors On Chemical Properties And Heavy Metals In Water For Livestock Farms In Thailand,
2013
Chulalongkorn University
Critical Factors On Chemical Properties And Heavy Metals In Water For Livestock Farms In Thailand, Teeraporn Makaroon, Suwicha Kasemsuwan, Nattavut Ratanavanichrojn, Hathairad Hananantachai, Rachaneekorn Mingkhwan, Sutha Khaodhiar, Phitsanu Tulayakul
The Thai Journal of Veterinary Medicine
This study aimed to investigate chemical quality of animal drinking used in livestock farms in Thailand. Samples and information from 68 pig farms, 35 chicken farms and 9 duckling farms were collected during March to August 2011. Water quality data were analyzed and compared with standard levels using the Mann-Whitney U and Wilcoxon Signed Rank Tests. Results revealed that median chemical values of water for livestock were generally within standard levels, except manganese and iron, which were detected in surfacewater with median values of 0.183 and 0.506 ppm, respectively, and are both higher than standard limits for drinking water. Median …
Clastogenic And Anticlastogenic Potential Of Neem Flower Extract Evaluated By Rat Liver Micronucleus Assay,
2013
Chulalongkorn University
Clastogenic And Anticlastogenic Potential Of Neem Flower Extract Evaluated By Rat Liver Micronucleus Assay, Piengchai Kupradinun, Yaninee Jarratwisarutporn, Rawiwan Wongpoomchai, Hathaitip Sritanaudomchai, Mati Rienkitjakarn
The Thai Journal of Veterinary Medicine
Clastogenic and anticlastogenic effects of neem flower (Azadirachta indica A. Juss) extract were determined using a rat liver micronucleus assay. Methanol extracts of neem flowers (MENF) in 15% Tween 80 at 100 and 500 mg/kg body weight were orally given to male Sprague Dawley rats daily for 6 weeks (2 weeks prior to and 4 weeks during repeated administration of diethylnitrosamine (DEN). All rats were anesthetized and their livers were collected. Hepatocytes were isolated from the livers using a collagenase solution without performing liver perfusion, then stained with 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI). Incidence of the micronucleated hepatocytes was evaluated by …
Field Efficacy Of Potassium Peroxymonosulfate (Pms) Oxidizing Disinfectant (Virusniptm) Against Porcine Circovirus Type 2 (Pcv2) In Gilt Acclimatization Unit,
2013
Chulalongkorn University
Field Efficacy Of Potassium Peroxymonosulfate (Pms) Oxidizing Disinfectant (Virusniptm) Against Porcine Circovirus Type 2 (Pcv2) In Gilt Acclimatization Unit, Korakrit Poonsuk, Jirapat Arunorat, Sasiwimon Talummuk, Rapeepat Kunalintip, Woravit Anuvongnukroh, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Virusnip™ is one of the commercial potassium peroxymonosulfate disinfectants widely used in swine farms. This disinfectant is well studied and proves to be excellent in antimicrobial activity previously. However, there is no information about its efficacy under field condition. Thus, the objective of this study was to determine the virucidal activity of Virusnip® against PCV2 in farm condition. Consequently, 1:200 concentration of VirusnipTM was sprayed for 15 minutes once or twice daily depending on the acclimatization period in gilts isolation units compared to the untreated group in the same building. Environmental swabs and oral fluid samples were collected and pooled …
Ecg Quiz,
2013
Chulalongkorn University
Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Thanusorn Phakhawambodee
The Thai Journal of Veterinary Medicine
No abstract provided.
Ultrasound Diagnosis,
2013
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
An In Vitro Comparative Study Of Aflatoxin B1 Adsorption By Thai Clay And Commercial Toxin Binders,
2013
Chulalongkorn University
An In Vitro Comparative Study Of Aflatoxin B1 Adsorption By Thai Clay And Commercial Toxin Binders, Bundit Tengjaroenkul, Urai Tengjaroenkul, Natapol Pumipuntu3, Komkrich Pimpukdee, Sawitree Wongtangtintan, Piyawat Saipan
The Thai Journal of Veterinary Medicine
Twenty samples of Thai clay from fourteen provinces and seven commercial toxin binders were investigated for their adsorption capacity of aflatoxin B1 (AFB1) in vitro. Each sample of 5 mg/l AFB1 solution was shaken at 250C for 24 hours and supernatants of centrifuged samples were analyzed for concentrations of AFB1 using a UV spectrophotometer. Adsorption capacity was calculated and applied to isothermic equations. The results indicated that Thai clays was capable of sequestering AFB1 from aqueous solution differently and Thai clay from Lopburi and Lamphun provinces had the highest adsorption capacity, similar to commercial binders. S-shaped isothermic curves were observed …
Efficacy And Safety Of Different Live Mycoplasma Gallisepticum Vaccines In Layer Chickens,
2013
Chulalongkorn University
Efficacy And Safety Of Different Live Mycoplasma Gallisepticum Vaccines In Layer Chickens, Somsak Pakpinyo, Suwarak Wanaratana, Kreangkrai Sangthongdang, Marcelo Paniago
The Thai Journal of Veterinary Medicine
The efficacy and safety of different live Mycoplasma gallisepticum (MG) vaccines were determined. One hundred and fifty six-week-old female layer chickens were equally divided into 6 groups. Groups 1-6 received F (two groups), 6/85 and ts-11 strains, positive and negative control, respectively. At 8 weeks old, 5 birds/group were sacrificed and gross thoracic airsac lesion scores (GTALS) and microscopic tracheal lesion scores (MTLS) were blindly evaluated. At 12 weeks old, groups 1-5 were challenged through an eye dropping with approximately 1x106 CFU of MG Thai isolated. At 13 and 14 weeks old, 10 birds/group were swabbed in the palatine fissure …
Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd,
2013
Chulalongkorn University
Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Sanipa Suradhat, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine circovirus type 2 (PCV2) causes multisystemic diseases called porcine circovirus associated disease (PCVAD) in pigs leading to a significant economic loss in the swine industry. The objective of this study was to determine the efficacy of a developed sub-unit PCV2b vaccine using a recombinant truncated capsid protein expressed in Escherichia coli in PCVAD-affected pig farms. Five-week-old conventional pigs were allocated into 2 groups: vaccinated (n = 55) and control (n = 55) groups placed in the same building. The vaccinated pigs were given two shots of a developed vaccine with 2-week interval on day 0 and 14. Serum samples …
Analysis Of Mirna-29a Expression In Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction,
2013
Chulalongkorn University
Analysis Of Mirna-29a Expression In Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction, Sunaree Nantakhruea, Porntippa Lekcharoensuk, Win Surachetpong
The Thai Journal of Veterinary Medicine
MicroRNAs (miRNAs) are small non coding RNA that regulate gene expression as post transcriptional regulators in mammalian tissues. One of the miRNAs that participates in mammalian immune response is miR-29a. Despite the limited information of miR-29a in porcine cells, it is interesting to determine whether miR-29a involves in immune regulation in pig. In this study, we developed a quantitative reverse transcription polymerase chain reaction using SYBR detection system to examine the expression of primary miR-29a (pri-miR-29a) in porcine peripheral blood mononuclear cells (PBMCs) in the presence of mitogen. PBMCs were stimulated with concanavalin A (Con A) at a concentration of …
Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China,
2013
Chulalongkorn University
Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China, Hui Lan Wu, Peng Hui Hu, Jin Zhang
The Thai Journal of Veterinary Medicine
Wenyonella philiplevinei is one of the important pathogens of duck coccidiasis. In the present study, a portion of the 18S rDNA was amplified from W. philiplevinei by Polymerase chain reaction (PCR), cloned and then sequenced. The sequences of 18S rDNA of all samples were 422 bp in size. A + T content of the 18S rDNA sequences was 58-59%. Sequence comparison revealed that the similarity in 18S rDNA sequences between Hunan isolates and W. philiplevinei available (Guangdong isolate) was more than 98%. Intra-specific sequence variations within each of the Hunan isolates were 0-1.7%. However, the inter-specific sequence differences between W. …
Ophthalmology Snapshot,
2013
Chulalongkorn University
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
What Is Your Diagnosis,
2013
Chulalongkorn University
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep, Rampaipat Tungjitpeanpong
The Thai Journal of Veterinary Medicine
No abstract provided.
Stress And Reproductive Hormones In Grizzly Bears Reflect Nutritional Benefits And Social Consequences Of A Salmon Foraging Niche,
2013
University of Victoria
Stress And Reproductive Hormones In Grizzly Bears Reflect Nutritional Benefits And Social Consequences Of A Salmon Foraging Niche, Heather M. Bryan, Chris T. Darimont, Paul C. Paquet, Katherine E. Wynne-Edwards, Judit E. G. Smits
Physiology Collection
Physiological indicators of social and nutritional stress can provide insight into the responses of species to changes in food availability. In coastal British Columbia, Canada, grizzly bears evolved with spawning salmon as an abundant but spatially and temporally constrained food source. Recent and dramatic declines in salmon might have negative consequences on bear health and ultimately fitness. To examine broadly the chronic endocrine effects of a salmon niche, we compared cortisol, progesterone, and testosterone levels in hair from salmon-eating bears from coastal BC (n = 75) with the levels in a reference population from interior BC lacking access to salmon …
Extreme Evolutionary Conservation Of Functionally Important Regions In H1n1 Influenza Proteome,
2013
Mississippi State University
Extreme Evolutionary Conservation Of Functionally Important Regions In H1n1 Influenza Proteome, Samantha Warren, Xiu-Feng Wan, Gavin Conant, Dmitry Korkin
CVM Publications
No abstract provided.
