Calving Assistance Influences The Occurrence Of Umbilical Cord Pathologies Treated Surgically In Calves,
2014
TÜBİTAK
Calving Assistance Influences The Occurrence Of Umbilical Cord Pathologies Treated Surgically In Calves, Carlos Moscuzza, Horacio Milicich, Guadalupe Alvarez, Betiana Gutierrez, Mariano Nahum
Turkish Journal of Veterinary & Animal Sciences
Ninety-two umbilical pathologies were surgically treated in Holstein, Aberdeen Angus, and Polled Hereford calves, both males and females, whose ages ranged between 2 and 8 months. The pathology diagnosis was performed considering the main anatomical elements involved in the umbilical cord, the inflammation progression, and the macroscopic characteristics of injuries. The umbilical cord pathologies were related to calving characteristics. The proportion of affected umbilical cords in calves born via assisted calving (77%) was significantly higher than that observed in calves born from normal parturition. The umbilical cord pathology of highest occurrence was omphalophlebitis (53.2%). A considerable proportion of infectious diseases …
Gallbladder Mucocele In A Dog: An Ultrasonography And Pathology Report,
2014
TÜBİTAK
Gallbladder Mucocele In A Dog: An Ultrasonography And Pathology Report, Marudhai Thangapandiyan, Ramasamy Sridhar, Kirubakaran Jeyaraja, Masilamani Sakthivelan, Thangaraj Mohanapriya, Natesan Pazhanivel
Turkish Journal of Veterinary & Animal Sciences
A 9-year old male, German shepherd dog was presented to the Madras Veterinary College Teaching Hospital with a history of a chronic ailment that had increased over the previous few months. A diagnosis of chronic renal failure and hepatitis was made and abdominal ultrasonography revealed the presence of gallbladder mucocele (GBM). The etiology, diagnosis, necropsy, and histopathological lesions were discussed.
Morphological And Morphometrical Characteristics Of The Interdigital Gland In Kivircik Sheep,
2014
TÜBİTAK
Morphological And Morphometrical Characteristics Of The Interdigital Gland In Kivircik Sheep, Yasi̇n Demi̇raslan, Yalçin Akbulut, Turgay Deprem, Ebru Karadağ Sari, Kadi̇r Aslan
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to determine the morphological and morphometric characteristics of the interdigital gland in Kivircik sheep. The interdigital glands in the forefeet and hind feet of the 16 male Kivircik sheep were examined in terms of morphometric and morphological characteristics. Four lengths (length of duct and corpus, diameter of duct and corpus) and 2 angles (angle of internal and external flexure) were measured in the interdigital glands located between 2 digits after dissection. The interdigital gland contained orifice, excretory duct, flexure, and corpus sections. Lengths and widths of corpus and excretory ducts of the interdigital gland …
Maternal Embryokines That Regulate Development Of The Bovine Preimplantation Embryo,
2014
TÜBİTAK
Maternal Embryokines That Regulate Development Of The Bovine Preimplantation Embryo, Peter James Hansen, Anna Carolina Denicol, Kyle Bradley Dobbs
Turkish Journal of Veterinary & Animal Sciences
Proper embryonic development depends upon regulatory signals from the reproductive tract called embryokines. This review uses the cow as a model to understand the developmental processes controlled by embryokines. The focus is on 3 embryokines that have been shown to increase competence of embryos to survive after transfer to recipients: CSF2, IGF1, and DKK1. Together, these molecules regulate key events required for development of the embryo to the blastocyst stage including proliferation (enhanced by IGF1), development of the inner cell mass (regulated by CSF2), control of differentiation (modified by DKK1), and inhibition of apoptosis and stress-mediated developmental arrest (IGF1 and …
Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects,
2014
TÜBİTAK
Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects, Iwona Szatkowska, Daniel Zaborski, Witold Stanislaw Proskura, Sara Tabor
Turkish Journal of Veterinary & Animal Sciences
Polledness intersex syndrome (PIS) is a phenomenon associated with 2 distinct conditions occurring in goats, namely polledness and intersexuality. The first is a trait exhibiting complete dominance, whereas the latter is recessive. The extreme case of intersexualism is complete sex reversal, which is observed only in females (60,XX). The trait PIS is inherited in a recessive manner and caused by deletion of 11.7 kbp DNA on chromosome 1q43. Female goats with PIS exhibit a broad range of developmental abnormalities of the sexual system, from only subtle changes to complete sex reversal. The phenotypic diversity in goats with PIS is most …
Application Of Dietary Fish Oil In Dairy Cow Reproduction,
2014
TÜBİTAK
Application Of Dietary Fish Oil In Dairy Cow Reproduction, Anna Zwyrzykowska, Robert Kupczynski
Turkish Journal of Veterinary & Animal Sciences
Feed fats are commonly used as a component of high-yielding cow diet rations. Many studies have been conducted on the effects of fat supplementation on metabolism, milk composition, and improvements in dairy cow reproduction. The positive effects on fat (vegetable oil) and energy supplementation on improvements in reproduction are well documented. Consumption of polyunsaturated fatty acids (PUFAs), including n-3 acids, in appropriate feed doses improves fertility of dairy cows. A fat source with high levels of acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is marine oil, especially fish and algae. By modification of cow rations, EPA and …
Molecular, Cellular, And Physiological Determinants Of Bull Fertility,
2014
TÜBİTAK
Molecular, Cellular, And Physiological Determinants Of Bull Fertility, Alexis Marie Parisi, Shannon Kate Thompson, Abdullah Kaya, Erdoğan Memi̇li̇
Turkish Journal of Veterinary & Animal Sciences
Bull fertility, defined as the ability of the spermatozoon to fertilize and activate the ovum and then support embryonic and even fetal development, is a crucial factor influencing animal reproduction and production efficiency. Despite significant influences of fertility on herd genetic improvement and efficient cattle production, the mechanisms regulating this phenotype and reliable biomarkers associated with bull fertility are poorly defined. Specifically, there is a broad lack of knowledge in bull fertility. In this paper, bull fertility is reviewed within the context of animal systems physiology, and system biology markers associated with bull fertility are provided. This mini-review is intended …
Cholesterol Efflux From Sperm: Approaches And Applications,
2014
TÜBİTAK
Cholesterol Efflux From Sperm: Approaches And Applications, Zahid Naseer, Ejaz Ahmad, Meli̇h Aksoy
Turkish Journal of Veterinary & Animal Sciences
Following ejaculation, sperm appear morphologically mature, but functionally they are inactive. To attain functional capability or capacitation in vivo, sperm must reside in the female reproductive tract for a certain period of time before fertilization. In the female reproductive tract, cholesterol efflux from the sperm plasma membrane is considered vital for the initiation of capacitation. A number of in vitro studies have used biological and nonphysiological means to understand the phenomenon of cholesterol efflux and capacitation. In this review, we present a short overview of the role of cholesterol efflux in regulating sperm capacitation using various agents. This review provides …
The Avian Ovary And Follicle Development: Some Comparative And Practical Insights,
2014
TÜBİTAK
The Avian Ovary And Follicle Development: Some Comparative And Practical Insights, Alan L. Johnson
Turkish Journal of Veterinary & Animal Sciences
This review focuses on recent research related to the avian ovary and, in particular, cellular mechanisms mediating embryonic growth of the ovary, postembryonic follicular development, and the process of follicular selection. Attention is drawn to a variety of fundamental, yet unresolved, aspects of ovarian function, several of which are uniquely related to avian reproductive strategies. Some notable differences in ovarian function between birds and the most closely related extant group, the crocodilians, are illustrated. Moreover, because the sauropsid (reptilian and avian) and synapsid (mammalian) lineages diverged shortly after the emergence of amniotes some 340 million years ago, consideration is given …
Endocrine And Metabolic Mechanisms Of Embryo And Fetal Intrauterine Growth Retardation In Dairy Cows,
2014
TÜBİTAK
Endocrine And Metabolic Mechanisms Of Embryo And Fetal Intrauterine Growth Retardation In Dairy Cows, Anatoliy Nezhdanov, Sergey Shabunin, Vitaliy Mikhalev, Nikolay Klimov, Anton Chernitskiy
Turkish Journal of Veterinary & Animal Sciences
This research was conducted to study hormonal and metabolic statuses and to determine ultrasound criteria for early diagnosis of fetal growth retardation syndrome (FGRS) in dairy cows of Black-motley (n = 41) and Red-motley (n = 56) Holstein breeds. Concentrations of progesterone, testosterone, 17beta-estradiol, dehydroepiandrosterone sulfate, cortisol, and triiodothyronine in blood serum were assessed by immune-enzyme analysis. Concentrations of plasma L-ascorbic acid and nitrogen oxides, serum immune globulin, middle molecular peptides, and bactericidal and lysozyme activity were determined by spectrophotometry. On days 38-40 and 60-65 of gestation, FGRS in cows was associated with hypoprogesteronemia, decrease of adrenal and thyroid gland …
The Effect Of Short-Term Lupin (Lupinus Angustifolius) Feed Supplementation On Serum Steroid Hormones, Insulin-Like Growth Factor I, And Ovarian Follicular Development And Atresia In Merino Ewes, Radoslava Vlckova, Jan Posivak, Igor Valocky, Zuzana Kravcova, Andrea Eibenová, Drahomia Sopkova
Turkish Journal of Veterinary & Animal Sciences
The effects of short-term lupin supplementation on ovulation rate in ewes during oestrous season are well known. In this study, we tested the effects of an 8-day lupin supplementation on folliculogenesis during anoestrus applied over the hormonal treatment of ewes. Fourteen anoestrous Merino ewes were assigned into 2 groups (n = 7 for each) in which the control group was fed a basal diet and the experimental group was supplemented with lupin groats (500 g per head per day). Both groups were treated intramuscularly with 12.5 µg/head lecirelinum (luteinising hormone releasing hormone super analogue) on the first day of supplementation …
Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability,
2014
TÜBİTAK
Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability, Babak Qasemi Panahi, Parviz Tajik, Mansoureh Movahedin, Gholamali Moghaddam, Mohammad Hossein Geranmayeh
Turkish Journal of Veterinary & Animal Sciences
Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1). During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 …
Expression Of Vascular Endothelial Growth Factor And Hypoxia-Inducible Factor-1 Alpha During The Periovulatory Period In Goats,
2014
TÜBİTAK
Expression Of Vascular Endothelial Growth Factor And Hypoxia-Inducible Factor-1 Alpha During The Periovulatory Period In Goats, Chainarong Navanukraw, Jiratti Thammasiri, Tossapol Moonmanee, Jaruk Nutthakornkul
Turkish Journal of Veterinary & Animal Sciences
To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1 alpha were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1 alpha at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1 alpha in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relationships of VEGF- and HIF-1 alpha -dependent angiogenesis during the periovulatory periods by which hypoxia during ovulation is crucial for establishing the thecal new vasculature.
Noninvasive Assessment Of Cardiac Abnormalities In Experimental Autoimmune Myocarditis By Magnetic Resonance Microscopy Imaging In The Mouse,
2014
University of Nebraska-Lincoln
Noninvasive Assessment Of Cardiac Abnormalities In Experimental Autoimmune Myocarditis By Magnetic Resonance Microscopy Imaging In The Mouse, Chandirasegara Massilamany, Vahid Khalilzad-Sharghi, Arunakumar Gangaplara, David Steffen, Shadi F. Othman, Jay Reddy
School of Veterinary and Biomedical Sciences: Faculty Publications
Myocarditis is an inflammation of the myocardium, but only ~10% of those affected show clinical manifestations of the disease. To study the immune events of myocardial injuries, various mouse models of myocarditis have been widely used. This study involved experimental autoimmune myocarditis (EAM) induced with cardiac myosin heavy chain (Myhc)-α 334-352 in A/J mice; the affected animals develop lymphocytic myocarditis but with no apparent clinical signs. In this model, the utility of magnetic resonance microscopy (MRM) as a non-invasive modality to determine the cardiac structural and functional changes in animals immunized with Myhc-α 334-352 is shown. EAM and healthy mice …
Sjl Mice Infected With Acanthamoeba Castellanii Develop Central Nervous System Autoimmunity Through The Generation Of Cross-Reactive T Cells For Myelin Antigens,
2014
School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln
Sjl Mice Infected With Acanthamoeba Castellanii Develop Central Nervous System Autoimmunity Through The Generation Of Cross-Reactive T Cells For Myelin Antigens, Jay Reddy, Chandirasegaran Massilamany, Francine Marciano-Cabral, Bruno Da Rocha-Azevedo, Melissa Jamerson, Arunakumar Gangaplara, David Steffen, Rana Zabad, Zsolt Illes, Raymond Sobel
School of Veterinary and Biomedical Sciences: Faculty Publications
We recently reported that Acanthamoeba castellanii (ACA), an opportunistic pathogen of the central nervous system (CNS) possesses mimicry epitopes for proteolipid protein (PLP) 139–151 and myelin basic protein 89–101, and that the epitopes induce experimental autoimmune encephalomyelitis (EAE) in SJL mice reminiscent of the diseases induced with their corresponding cognate peptides. We now demonstrate that mice infected with ACA also show the generation of crossreactive T cells, predominantly for PLP 139–151, as evaluated by T cell proliferation and IAs/dextramer staining. We verified that PLP 139–151-sensitized lymphocytes generated in infected mice contained a high proportion of T helper 1 cytokineproducing cells, …
Protective Humoral Immune Response Induced By An Inactivated
Porcine Reproductive And Respiratory Syndrome Virus Expressing
The Hypo-Glycosylated Glycoprotein 5,
2014
Konkuk University, Seoul
Protective Humoral Immune Response Induced By An Inactivated Porcine Reproductive And Respiratory Syndrome Virus Expressing The Hypo-Glycosylated Glycoprotein 5, Jung-Ah Lee, Byungjoon Kim, Fernando A. Osorio, Asit K. Pattnaik, Nak-Hyung Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee
School of Veterinary and Biomedical Sciences: Faculty Publications
Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry worldwide. Although inactivated and live vaccines are commercially available for the control of PRRS, both types of vaccine have not always proven successful in terms of generating a protective immune response, particularly in the case of inactivated vaccines. In this study, we tested whether an inactivated vaccine could induce a humoral immune response to PRRS during a homologous challenge. Amino acid substitutions were introduced into glycoprotein (GP) 5 of the FL12 strain of the PRRS virus (PRRSV) using site-directed mutagenesis with a pFL12 infectious clone. The …
Light Stimulates The Mouse Adrenal Through A
Retinohypothalamic Pathway Independent Of An Effect
On The Clock In The Suprachiasmatic Nucleus,
2014
University of Nebraska - Lincoln
Light Stimulates The Mouse Adrenal Through A Retinohypothalamic Pathway Independent Of An Effect On The Clock In The Suprachiasmatic Nucleus, Silke Kiessling, Patricia J. Sollars, Gary E. Pickard
School of Veterinary and Biomedical Sciences: Faculty Publications
The brain’s master circadian pacemaker resides within the hypothalamic suprachiasmatic nucleus (SCN). SCN clock neurons are entrained to the day/night cycle via the retinohypothalamic tract and the SCN provides temporal information to the central nervous system and to peripheral organs that function as secondary oscillators. The SCN clock-cell network is thought to be the hypothalamic link between the retina and descending autonomic circuits to peripheral organs such as the adrenal gland, thereby entraining those organs to the day/night cycle. However, there are at least three different routes or mechanisms by which retinal signals transmitted to the hypothalamus may be conveyed …
Moraxella Spp. Isolated From Field Outbreaks Of Infectious Bovine Keratoconjunctivitis: A Retrospective Study Of Case Submissions From 2010 To 2013,
2014
University of Nebraska-Lincoln
Moraxella Spp. Isolated From Field Outbreaks Of Infectious Bovine Keratoconjunctivitis: A Retrospective Study Of Case Submissions From 2010 To 2013, John Dustin Loy, Bruce W. Brodersen
School of Veterinary and Biomedical Sciences: Faculty Publications
Infectious bovine keratoconjunctivitis (IBK), also known as pinkeye, is the most costly eye disease of cattle. The principal etiologic agent of IBK is the Gram-negative bacterium Moraxella bovis. However, there have been reports of IBK outbreaks associated with Moraxella bovoculi. A retrospective study of IBK diagnostic cases submitted from July 1, 2010, through October 31, 2013, was conducted. Included in the study were 1,042 Moraxella isolates from 1,538 swabs of lacrimal secretions collected from 282 herds from 30 U.S. states. Moraxella isolates were identified to the species level and were composed of M. bovoculi (701 isolates), M. bovis …
Bovine Herpesvirus 1 Productive Infection Stimulates Inflammosome Formation And Caspase 1 Activity,
2014
Qingdao University
Bovine Herpesvirus 1 Productive Infection Stimulates Inflammosome Formation And Caspase 1 Activity, Jianlin Wang, Jeffrey Alexander, Matthew S. Wiebe, Clinton J. Jones
Nebraska Center for Virology: Faculty Publications
Bovine herpesvirus 1 (BoHV-1), a significant viral pathogen of cattle, causes inflammation in affected tissue during acute infection. Consequently, we tested whether productively infected bovine cells stimulate inflammasome formation. Expression of two components required for inflammasome formation, the DNA sensor IFI16 (gamma-interferon-inducible protein 16) and NLRP3 (NOD-like receptor family, pyrin domain containing 3), were induced in bovine kidney cells by eight hours after infection. IFI16 was detected in punctate granules localized to the cytoplasm and nucleus. During productive infection, more than ten times more cells were caspase 1 positive, which is activated following inflammasome formation. Two caspase 1 inhibitors had …
Experimental Infection Of Pregnant Goats With Bovine Viral Diarrhea Virus (Bvdv) 1 Or 2,
2014
Auburn University
Experimental Infection Of Pregnant Goats With Bovine Viral Diarrhea Virus (Bvdv) 1 Or 2, Thomas Passler, Kay P. Riddell, Misty A. Edmondson, Manuel F. Chamorro, John D. Neill, Bruce W. Brodersen, Heather L. Walz, Patricia K. Galik, Yijing Zhang, Paul H. Walz
School of Veterinary and Biomedical Sciences: Faculty Publications
Infections with bovine viral diarrhea virus (BVDV) of the genus pestivirus, family Flaviviridae, are not limited to cattle but occur in various artiodactyls. Persistently infected (PI) cattle are the main source of BVDV. Persistent infections also occur in heterologous hosts such as sheep and deer. BVDV infections of goats commonly result in reproductive disease, but viable PI goats are rare. Using 2 BVDV isolates, previously demonstrated to cause PI cattle and white-tailed deer, this study evaluated the outcome of experimental infection of pregnant goats. Pregnant goats (5 goats/group) were intranasally inoculated with BVDV 1b AU526 (group 1) or BVDV 2 …
