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Serological And Molecular Characterization Of Streptococcus Iniae In Cultured Nile Tilapia (Oreochromis Niloticus) In Thailand, Sasibha Jantrakajorn, Mintra Lukkana, Janenuj Wongtavatchai 2014 Chulalongkorn University

Serological And Molecular Characterization Of Streptococcus Iniae In Cultured Nile Tilapia (Oreochromis Niloticus) In Thailand, Sasibha Jantrakajorn, Mintra Lukkana, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

Streptococcus iniae infections developed in on-growing tilapia farms located in the northeastern and central regions of Thailand during 2003-2011. In the present study, S. iniae isolates recovered from diseased tilapia were investigated using biochemical, serological and molecular techniques. Biochemical characterization showed different profiles. The arginine dihydrolase (ADH) activity was distinctive between S. iniae, isolates obtained from the outbreaks in central region were ADH-positive (ADH+ve) while northeastern isolates were ADH-negative (ADH-ve). The serological assay demonstrated corresponding results to the ADH activity. Serotype I and serotype II were ascribed to the ADH+ve and ADH-ve isolates, respectively. Despite the serotypic differences, phylogenetic analysis …


Endocrine And Metabolic Status Of Dairy Cows During Transition Period, Radojica Djoković, Marko Cincović, Vladimir Kurćubić, Milun Petrović, Miroslav Lalović, Boban Jašović, Zoran Stanimirovic 2014 Chulalongkorn University

Endocrine And Metabolic Status Of Dairy Cows During Transition Period, Radojica Djoković, Marko Cincović, Vladimir Kurćubić, Milun Petrović, Miroslav Lalović, Boban Jašović, Zoran Stanimirovic

The Thai Journal of Veterinary Medicine

The objective of this study was to determine blood levels of cortisol, insulin, triiodothyronine (T3), thyroxine (T4), glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate (BHB), triglycerides (TG) and total cholesterol in dairy Holstein cows (n = 40) during the transition period. The test cows were classified into four groups: Group A (n = 10), clinically healthy late-pregnant cows from day 20 to day 10 before calving. Group B (n = 10), clinically healthy late-pregnant cows from day 10 to day 1 before calving. Group C (n = 10), clinically healthy puerperal cows. Group D (n = 10), puerperal ketotic cows. The …


Clinical Effect Of Glucosamine And Chondroitin Contained Nutraceutical On Osteoarthritis In Dogs After Anterior Cruciate Ligament Rupture Surgical Repair, Prach Maihasap, Kumpanart Soontornwipart, Natee Techaarpornkul 2014 Chulalongkorn University

Clinical Effect Of Glucosamine And Chondroitin Contained Nutraceutical On Osteoarthritis In Dogs After Anterior Cruciate Ligament Rupture Surgical Repair, Prach Maihasap, Kumpanart Soontornwipart, Natee Techaarpornkul

The Thai Journal of Veterinary Medicine

Glucosamine and chondroitin sulfates arekey structural components of extracellular fluid in articular cartilage. From previous studies, glucosamine and chondroitin sulfates have several positive effects on cartilage homeostasis and are used in osteoarthritis patients. Although glucosamine and chondroitin sulfates have some positive effects on clinical treatment of osteoarthritis, clinical results still need to be investigated. The objective of this study was to investigate the clinical outcomes on lameness score, radiological score, serum WF6 level and serum HA level of glucosamine and chondroitin sulfates in osteoarthritic dogs, using anterior cruciate ligament rupture patients as study models of osteoarthritis (n=12). The results from …


Isoflurane Concentration When Compared With Intramuscular Morphine During Patellar Luxation Repair In Dogs, Pathompong Thapun, Sumit Durongphongtorn, Marissak Kalpravidh 2014 Chulalongkorn University

Isoflurane Concentration When Compared With Intramuscular Morphine During Patellar Luxation Repair In Dogs, Pathompong Thapun, Sumit Durongphongtorn, Marissak Kalpravidh

The Thai Journal of Veterinary Medicine

This clinical trial aimed to assess the intraoperative analgesic effect, based on the required minimum alveolarconcentration (MAC) of isoflurane, of transdermal fentanyl when compared with intramuscular morphine in dogsundergoing patellar luxation repair. Seventeen healthy dogs with grade II or grade III of medial patellar luxationwere randomly assigned to group M or group F, receiving a sham patch or a transdermal fentanyl patch (25 μg/h),respectively, applied on the skin at least 24 h prior to surgery. Three dogs had bilateral medial patellar luxation andwere then enrolled in both groups, resulting in ten animals for each group. Before surgery, group M dogs …


Evaluation Of Wing Fractures On Survival And Re-Release, Katherine O'Neill 2014 California Polytechnic State University, San Luis Obispo

Evaluation Of Wing Fractures On Survival And Re-Release, Katherine O'Neill

Animal Science

No abstract provided.


Sources And Disseminations Of Salmonella Spp. In An Integrated Broiler Meat Production, Nion Boonprasert, Suphachai Nuanualsuwan, Chaiwat Pulsrikarn, Sarinya Pornaem, Nipa Chokesajjawatee 2014 Chulalongkorn University

Sources And Disseminations Of Salmonella Spp. In An Integrated Broiler Meat Production, Nion Boonprasert, Suphachai Nuanualsuwan, Chaiwat Pulsrikarn, Sarinya Pornaem, Nipa Chokesajjawatee

The Thai Journal of Veterinary Medicine

In this study, important sources and disseminations of Salmonella in integrated broiler meat production was conducted in the north-eastern part of Thailand during 2010-2012. Both environmental samples and chicken-related samples from three broiler meat production cycles, starting from breeder farm, hatchery, broiler farm, and slaughterhouse were collected. The chicken-related samples were tested to monitor Salmonella status of the chickens, eggs, or whole carcasses, whereas the environmental samples were tested to investigate the possible sources of Salmonella contamination. A total of 1,449 chicken-related samples and 802 environmental samples were analyzed. Results of this study showed that Salmonella was found in all …


Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk 2014 Chulalongkorn University

Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk

The Thai Journal of Veterinary Medicine

MicroRNAs are a group of small non-coding RNA that are involved in posttranscriptional regulation through target mRNA degradation or inhibition of protein synthesis. The objective of this present study was to characterize and determine expression level of porcine miR-29a in various tissues. Our results indicated that porcine miR-29a shared high conserved sequence with miR-29a from other organisms. Furthermore, in silico analysis suggested that the seed region of porcine miR-29a partially matched IFN-γ mRNA. RT-PCR analysis indicated that miR-29a expressed in various tissues including lymph node, spleen, peripheral blood mononuclear cells (PBMCs) and PK-15 cells. Expression profiling of miR-29a in PRRSV-infected …


Recurrent Rumen Tympany Caused By Trichobezoars In Buffaloes (Bubalus Bubalis): A Series Report, Ashraf M. Abu-Seida, Oday S. Al-Abbadi 2014 Chulalongkorn University

Recurrent Rumen Tympany Caused By Trichobezoars In Buffaloes (Bubalus Bubalis): A Series Report, Ashraf M. Abu-Seida, Oday S. Al-Abbadi

The Thai Journal of Veterinary Medicine

This series report was carried out for 3 years on 15 buffaloes suffering from recurrent rumen tympany associated with trichobezoars. Full case history, thorough clinical examination, ferroscopy, and vaginal and trans-rectal examination were carried out in all cases. The affected buffaloes were treated with rumenotomy. All cases were lactating buffaloes and had heavy hair coat. The age of affected buffaloes ranged between 5-10 years. Clinical signs included anorexia, depression, sharp decrease in milk yield, intermittent respiratory distress, recurrent rumen tympany and dehydration. Hematological and biochemical analyses revealed leukocytosis, hypokalemia and hypochloremia. Rumenotomy was curative in all cases and revealed trichobezoars …


Effects Of Effective Microorganisms On Growth Performances, Ammonia Reduction, Hematological Changes And Shedding Of Salmonella Enterica And Campylobacter Spp. In Broilers, Chackrit Nuengjamnong, Taradon Luangtongkum 2014 Chulalongkorn University

Effects Of Effective Microorganisms On Growth Performances, Ammonia Reduction, Hematological Changes And Shedding Of Salmonella Enterica And Campylobacter Spp. In Broilers, Chackrit Nuengjamnong, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

The aim of this study was to determine the effects of effective microorganisms (EM) product on ammonia reduction, growth performances, Salmonella and Campylobacter reduction and hematological changes in broilers. Five hundred and ninety four day-old female Arbor Acres were divided into 3 groups with 6 replicates in each group. Group 1 was the control group, while groups 2 and 3 received EM product in forms of daily spraying at 1:50 EM solution/m2 on litter and in drinking water at 1:800 dilution, respectively. Ammonia concentrations were measured weekly, while blood samples were collected at 21 and 42 days of age for …


Ophthalmology Snapshot, Nalinee Tuntivanich 2014 Chulalongkorn University

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan 2014 Chulalongkorn University

Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan

The Thai Journal of Veterinary Medicine

Identification of X or Y chromosome is a very useful technique to verify the sex of boar sperm, but common methods used in pig such as Fluorescence In situ Hybridisation (FISH) and whole semen Polymerase Chain Reaction (PCR) have some limitations. FISH is highly accurate, but time-consuming (>3 days). Whole semen PCR is faster than FISH (3-6 h), but not highly accurate (approximate methods). The objective of this study was to develop a fast and highly accurate protocol to identify sex of boar sperm. In the present study, our team developed an alternative sex identification protocol using single cell …


Perspectives On Antimicrobial Resistance In Livestock And Livestock Products In Asean Countries, Amornthep Archawakulathep, Chung Ta Thi Kim, Dethaloun Meunsene, Didik Handijatno, Hasliza Abu Hassim, Hope R. G. Rovira, Khin Sandar Myint, Loinda R. Baldrias, Meas Sothy, Min Aung, Nenny H. Wahyu, Rortana Chea, Sookruetai Boonmasawai, Soulasack Vannamahaxay, Sunpetch Angkititrakul, Therese M. A. Collantes, Tho Nguyen Van, Veerasak Punyapornwithaya, Zunita Zakaria, Rungtip Chuanchuen 2014 Chulalongkorn University

Perspectives On Antimicrobial Resistance In Livestock And Livestock Products In Asean Countries, Amornthep Archawakulathep, Chung Ta Thi Kim, Dethaloun Meunsene, Didik Handijatno, Hasliza Abu Hassim, Hope R. G. Rovira, Khin Sandar Myint, Loinda R. Baldrias, Meas Sothy, Min Aung, Nenny H. Wahyu, Rortana Chea, Sookruetai Boonmasawai, Soulasack Vannamahaxay, Sunpetch Angkititrakul, Therese M. A. Collantes, Tho Nguyen Van, Veerasak Punyapornwithaya, Zunita Zakaria, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

Antimicrobial resistance (AMR) in bacteria, particularly in foodborne pathogens, has increasingly become apparent in most parts of the world including ASEAN countries, creating great impact on economy, human and animal health and international food trade. The tendency of bacterial pathogens to become multidrug resistant (MDR) is the most serious concern in AMR. The ASEAN Economic Community (AEC) will be fully established in 2015 and ASEAN cooperation has acknowledged the significance of food safety, of which AMR is one of the major concerns. There is a need for all ASEAN nations to harmonize its AMR monitoring and surveillance programmes in order …


Reintroduction And Behavioral Observations Of Chinese Gorals (Naemorhedus Griseus) In Natural Conditions, Nithidol Buranapim, Narit Sitasuwan, Adisorn Kongprempoon, Keeta Korkusol, Boripat Siriaroonrat, Sumate Kamolnorranath 2014 Chulalongkorn University

Reintroduction And Behavioral Observations Of Chinese Gorals (Naemorhedus Griseus) In Natural Conditions, Nithidol Buranapim, Narit Sitasuwan, Adisorn Kongprempoon, Keeta Korkusol, Boripat Siriaroonrat, Sumate Kamolnorranath

The Thai Journal of Veterinary Medicine

The study of the ecology and behavior of gorals (Naemorhedus griseus) was conducted in Maelao-Maesae Wildlife Sanctuary, Chiang Mai Province during July 2012 – July 2013. The behavioral patterns of the gorals were observed using focal-scan sampling and descriptive methods. Results showed that the seven most common behavioral patterns were standing, walking, resting, grooming, scratching, feeding (foraging), and ruminating. Twelve plants were recognized as goral food based on direct field observations. Overall land area used by the gorals in this study was 81,124 m2. The highest number of gorals was found in dipterocarp forests, followed by deciduous forests and the …


Quantitative Microbial Risk Assessment Of Salmonella In Surface Water As A Source Of Tap Water, Paweeranut Banmairuroy, Phattharaporn Chaichana, Chaiwat Pulsrikarn, Suphachai Nuanualsuwan 2014 Chulalongkorn University

Quantitative Microbial Risk Assessment Of Salmonella In Surface Water As A Source Of Tap Water, Paweeranut Banmairuroy, Phattharaporn Chaichana, Chaiwat Pulsrikarn, Suphachai Nuanualsuwan

The Thai Journal of Veterinary Medicine

Salmonella spp. is fecal-orally transmitted and readily spread into the environment. Little is known about risk of utilizing surface water in Thailand. Quantitative microbial risk assessment has been used to estimate the risk of waterborne salmonellosis. For hazard characterization, beta-Poisson model was employed to describe the relationship between dose of Salmonella from water exposure and salmonellosis. Prevalence and concentration of Salmonella in the surface water including water exposure were used to model probability of exposure. The probabilistic prevalence was described by beta distribution and the range of mean Salmonella prevalences of the surface water along rivers were between 8.33% and …


Temperature, Hypoxia, And Mycobacteriosis: Effects On Adult Striped Bass Morone Saxatilis Metabolic Performance, Dominique Lapointe, Wolfgang K. Vogelbein, Mary C. Fabrizio, David T. Gauthier, Richard W. Brill 2014 Old Dominion University

Temperature, Hypoxia, And Mycobacteriosis: Effects On Adult Striped Bass Morone Saxatilis Metabolic Performance, Dominique Lapointe, Wolfgang K. Vogelbein, Mary C. Fabrizio, David T. Gauthier, Richard W. Brill

Biological Sciences Faculty Publications

Mycobacteriosis, a chronic bacterial disease of fishes, is prevalent in adult striped bass from Chesapeake Bay (USA). Although environmental factors may play a role in disease expression, the interaction between the disease and environmental stress remains unexplored. We therefore examined the individual and interactive effects of elevated temperature, hypoxia, and mycobacteriosis on the metabolism of wild-caught adult striped bass from Chesapeake Bay using respirometry. Because the spleen is the primary target organ of mycobacteriosis in striped bass, we hypothesized that the disease interferes with the ability of fish to increase their hemato crit in the face of increasing oxygen demands. …


Nebraska Bqa: Antibiotic Use Guidelines, Rob Eirich 2014 University of Nebraska-Lincoln

Nebraska Bqa: Antibiotic Use Guidelines, Rob Eirich

Nebraska Beef Quality Assurance Program

Cattlemen have demonstrated a commitment to animal health through vaccination programs developed carefully in cooperation with their veterinarian. When animals must be treated with an antibiotic after disease diagnosis, producers need to follow important antibiotic use guidelines established through the National Beef Quality Assurance (BQA) program.


Nebraska Bqa: Effective Cattle Handling, Rob Eirich 2014 University of Nebraska-Lincoln

Nebraska Bqa: Effective Cattle Handling, Rob Eirich

Nebraska Beef Quality Assurance Program

Cattlemen know the importance of proper cattle handling and its effect on animal health and performance. Beef Quality Assurance has been implementing effective cattle handling (low-stress) into educational programs since its establishment in the 1980s.


Nebraska Bqa: Handling Compromised Animals, Rob Eirich 2014 University of Nebraska-Lincoln

Nebraska Bqa: Handling Compromised Animals, Rob Eirich

Nebraska Beef Quality Assurance Program

Cattle producers have long recognized the need of proper husbandry and stewardship for the animals entrusted into their care. Best livestock production practices, based on research and sound science, help ensure the well-being, animal health and productivity of cattle.


Calf Branding/Processing Time And Beef Quality Assurance, Rob Eirich 2014 University of Nebraska-Lincoln

Calf Branding/Processing Time And Beef Quality Assurance, Rob Eirich

Nebraska Beef Quality Assurance Program

It is spring and that means branding time across cattle country. As producers are finishing calving season and preparing for branding/processing, it is important to review Beef Quality Assurance (BQA) guidelines. Let's take a minute to look at the key BQA principles:


Audits And Assessments In Beef Cattle Production, Rob Eirich 2014 University of Nebraska-Lincoln

Audits And Assessments In Beef Cattle Production, Rob Eirich

Nebraska Beef Quality Assurance Program

Assessments and audits are becoming a part of doing business in beef cattle production, as we are seeing beef processors implementing third-party audits. Since beginning as the Nebraska Director of Beef Quality Assurance (BQA) in July, I have been studying and gathering information on the National Cattlemen's Beef Association (NCBA) Assessment Guides and third-party audits in the beef industry.


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