Veterinary Medicine Commons^™

Approaches To Canine Health Surveillance, Dan G. O'Neill, David B. Church, Paul D. Mcgreevy, Peter C. Thomson, Dave C. Brodbelt

Epidemiology Collection

Effective canine health surveillance systems can be used to monitor disease in the general population, prioritise disorders for strategic control and focus clinical research, and to evaluate the success of these measures. The key attributes for optimal data collection systems that support canine disease surveillance are representativeness of the general population, validity of disorder data and sustainability. Limitations in these areas present as selection bias, misclassification bias and discontinuation of the system respectively. Canine health data sources are reviewed to identify their strengths and weaknesses for supporting effective canine health surveillance. Insurance data benefit from large and well-defined denominator populations …

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1- Porcine Epidemic Diarrhea Virus Testing and Research in High Demand at SDSU’s ADRDL [Page] 1- Clinical Signs Associated with Swine Deltacoronavirus in a Northeastern Nebraska Sow Herd [Page] 2- Director's Message: ADRDL Contributes to Animal Health Locally, Nationally, and Globally [Page] 3- Dr. Step Highlights Successful Bailey Herd Health Conference [Page] 4- Detecting and Discovering Viruses at SDSU’s ADRDL [Page] 6- Post Exposure Rabies Treatment for People Currently Rabies-Vaccinated [Page] 7- Antimicrobial Resistance the Subject of Latest South Dakota “One Health” Workshop Group Meeting [Page] 6- New Hires [Page] 6- New Hire: Grant Miller [Page] 7- Continuing Education …

Evidence Of Positive Selection In Mitochondrial Complexes I And V Of The African Elephant., Tabitha M. Finch, Nan Zhao, Dmitry Korkin, Katy H. Frederick, Lori S. Eggert

CVM Publications

As species evolve, they become adapted to their local environments. Detecting the genetic signature of selection and connecting that to the phenotype of the organism, however, is challenging. Here we report using an integrative approach that combines DNA sequencing with structural biology analyses to assess the effect of selection on residues in the mitochondrial DNA of the two species of African elephants. We detected evidence of positive selection acting on residues in complexes I and V, and we used homology protein structure modeling to assess the effect of the biochemical properties of the selected residues on the enzyme structure. Given …

Cd8Α Dendritic Cells Drive Establishment Of Hsv-1 Latency, Kevin R. Mott, Sariah J. Allen, Mandana Zandian, Bindu Konda, Behrooz G. Sharifi, Clinton Jones, Steven L. Wechsler, Terrence Town, Homayon Ghiasi

School of Veterinary and Biomedical Sciences: Faculty Publications

It is generally accepted that CD8 T cells play the key role to maintain HSV-1 latency in trigeminal ganglia of ocularly infected mice. Yet, comparably little is known about the role of innate immunity in establishment of viral latency. In the current study, we investigated whether CD8α DCs impact HSV-1 latency by examining latency in the trigeminal ganglia (TG) of wildtype (WT) C57BL/6 versus CD8α^‒/‒ (lack functional CD8 T cells and CD8α⁺ DCs), CD8β^‒/‒ (have functional CD8α⁺ T cells and CD8α⁺ DCs), and β2m^‒/‒ (lack functional CD8 T cells but have CD8α^{+ …}

The Role Of Sirtuin 1 And Ceramide In T10C12 Conjugated Linoleic Acid Induced Delipidation In 3t3-L1 Adipocytes, Wei Wang

Department of Animal Science: Dissertations, Theses, and Student Research

Project 1: Trans-10, cis-12 conjugated linoleic acid (t10c12 CLA) reduces triglyceride (TG) levels in adipocytes through multiple pathways, with AMP-activated protein kinase (AMPK) generally facilitating, and peroxisome proliferator-activated receptor γ (PPARγ) generally opposing these reductions. Sirtuin 1 (SIRT1), a histone/protein deacetylase that affects energy homeostasis, often functions coordinately with AMPK, and is capable of binding to PPARγ, thereby inhibiting its activity. This study investigated the role of SIRT1 in the response of 3T3-L1 adipocytes to t10c12 CLA by testing the following hypotheses: 1) SIRT1 is functionally required for robust TG reduction; …

Growing South Dakota (Spring 2014), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the SDSU Extension 2013 Annual Report.

[Page] 2 The New SDSU Extension: Two Years Later, Change Has Brought New Successes
[Page] 4 Your 24-7 Connection: iGrow.org Makes SDSU Extension Easily Accessible, Responsive
[Page] 5 SDSU Regional Extension Centers: Your Front Door To SDSU
[Pages] 6-7 Leading The Challenge: Projects To Address Food Security, Financial Knowledge Underway
[Pages] 8-9 Regional Collaboration: SET Grants & Food Networks Initiated
[Pages] 10-11 Encouraging & Empowering: Native American Program Assists Reservations In Striving For Healthy Food, Healthy Communities & Youth Science Programming
[Page] 12 Evaluating Yields: Crop Performance Testing Provides Important, Unbiased …

Overexpression Of Thioredoxin Binding Protein (Tbp-2) Increases Oxidation Sensitivity And Apoptosis In Human Lens Epithelial Cells, Yibo Yu, Kuiyi Xing, Rilwan Badamas, Charles A. Kuszynski, Hongli Wuand, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Thioredoxin (Trx) is an important redox regulator with cytosolic Trx1 and mitochondrial Trx2 isozymes. Trx has multi-physiological functions in cells and its bioavailability is negatively controlled through active site binding to a specific thioredoxin binding protein (TBP-2). This paper describes the delicate balance between TBP-2 and Trx, and the effect of overexpression of TBP-2 in the human lens epithelial cells. Cells overexpressing TBP-2 (TBP-2 OE) showed a 7- fold increase of TBP-2, and a nearly 40% suppression of Trx activity but no change in Trx expression. The TBP-2 OE cells grew slower and their population decreased to 30% by day …

Forecasting The Spread Of Raccoon Rabies Using A Purpose-Specific Group Decision-Making Process., Aaron M. Anderson, Stephanie A. Shwiff, Richard B. Chipman, Todd C. Atwood, Tyler Cozzens, Frank Fillo, Robert Hale, Brody Hatch, Joanne Maki, Olin E. Rhodes Jr., Erin E. Rees, Charles E. Rupprecht, Rowland Tinline, Kurt C. Vercauteren, Dennis Slate

United States Department of Agriculture Wildlife Services: Staff Publications

The Centers for Disease Control (CDC) and USDA Wildlife Services (WS) have been involved in an oral rabies vaccination (ORV) program for raccoons (Procyon lotor) that has slowed the westward spread of raccoon rabies. The objective of this study was to forecast the spread of the disease if an ORV zone was not maintained. A group decision-making process was designed to address the forecasting problem and was implemented using a group of 15 experts and 4 support personnel at a meeting at the USDA National Wildlife Research Center. Ten expansion regions were constructed that described the spread of …

Evaluation Of Novel Oral Vaccine Candidates And Validation Of A Caprine Model Of Johne’S Disease, Murray E. Hines Ii, Sue E. Turnquist, Marcia R.S. Ilha, Sreekumari Rajeev, Arthur L. Jones, Lisa Whittington, John P. Bannantine, Raul G. Barletta, Yrjö T. Gröhn, Robab Katani, Adel M. Talaat, Lingling Li, Vivek Kapur

School of Veterinary and Biomedical Sciences: Faculty Publications

Johne’s disease (JD) caused by Mycobacterium avium subspecies paratuberculosis (MAP) is a major threat to the dairy industry and possibly some cases of Crohn’s disease in humans. A MAP vaccine that reduced of clinical disease and/or reduced fecal shedding would aid in the control of JD. The objectives of this study were (1) to evaluate the efficacy of 5 attenuated strains of MAP as vaccine candidates compared to a commercial control vaccine using the protocol proposed by the Johne’s Disease Integrated Program (JDIP) Animal Model Standardization Committee (AMSC), and (2) to validate the AMSC Johne’s disease go at challenge model. …

Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman

Animal Science

No abstract provided.

Characterization Of A Novel Influenza Virus In Cattle And Swine: Proposal For A New Genus In The Orthomyxoviridae Family, Ben M. Hause, Emily A. Collin, Runxia Liu, Bing Huang, Zizhang Sheng, Wuxun Lu, Dan Wang, Eric A. Nelson, Feng Li

Veterinary and Biomedical Sciences Faculty Publications

We have recently reported the isolation of a novel virus, provisionally designated C/swine/Oklahoma/1334/2011 (C/OK), with 50% overall homology to human influenza C viruses (ICV), from a pig in Oklahoma. Deep RNA sequencing of C/OK virus found a matrix 1 (M1) protein expression strategy that differed from that of ICV. The novelty of C/OK virus prompted us to investigate whether C/OK virus could exist in a nonswine species. Significantly, we found that C/OK virus was widespread in U.S. bovine herds, as demonstrated by reverse transcription (RT)-PCR and serological assays. Genome sequencing of three bovine viruses isolated from two herds in different …

Prevalence Of Disorders Recorded In Dogs Attending Primary-Care Veterinary Practices In England, Dan G. O'Neill, David B. Church, Paul D. Mcgreevy, Peter C. Thomson, Dave C. Brodbelt

Epidemiology Collection

Purebred dog health is thought to be compromised by an increasing occurence of inherited diseases but inadequate prevalence data on common disorders have hampered efforts to prioritise health reforms. Analysis of primary veterinary practice clinical data has been proposed for reliable estimation of disorder prevalence in dogs. Electronic patient record (EPR) data were collected on 148,741 dogs attending 93 clinics across central and south-eastern England. Analysis in detail of a random sample of EPRs relating to 3,884 dogs from 89 clinics identified the most frequently recorded disorders as otitis externa (prevalence 10.2%, 95% CI: 9.1–11.3), periodontal disease (9.3%, 95% CI: …

Evaluation Of Wing Fractures On Survival And Re-Release, Katherine O'Neill

Animal Science

No abstract provided.

Recombinant Human Follicle Stimulating Hormone And Growth Factors Improve The Meiotic And Developmental Competence Of Cat Oocytes, Theerawat Tharasanit, Chommanart Thongkittidilok, Thanida Sananmuang, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

In vitro embryo production has proven as potential tool for understanding factors involving developmental biology of embryos. This study aimed at examining the effects of recombinant human follicle stimulating hormone (rhFSH) on meiotic resumption and also on developmental competence of cat oocytes. Experiment 1 studied mRNA expression of luteinizing hormone (LH), follicle stimulating hormone (FSH), epidermal growth factor (EGF) and insulin like growth factor-1 (IGF-1) receptors (R) in cumulus cells. mRNA expression of LH receptor in cumulus cells after oocyte maturation was additionally examined. Experiment 2 examined the effect of different rhFSH concentrations (0.01, 0.05, 0.1 and 1 IU/ml) on …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Relationship Between Rr And Qt Intervals In Normal And Pacing-Induced Heart Failure Dogs, Vudhiporn Limprasutr, Nakkawee Saengklub, Suwanakiet Sawangkoon, Chollada Buranakarl, Narongsak Chaiyabutr, Anusak Kijtawornrat

The Thai Journal of Veterinary Medicine

This study was conducted to determine the effects of rapid ventricular pacing on cardiac function and hemodynamics, and to determine the most suitable QTc formula for the effects of alterations in the RR interval on the QT interval of conscious dog with heart failure (HF). Electrocardiograms were acquired from dogs (n=18) at baseline and after rapid ventricular pacing (6, 9, 12 weeks). RR and QT intervals were measured and QTc intervals were calculated using 3 common formulae (Bazett, Fridericia and Van de Water). HF was confirmed by reduced left ventricular fractional shortening (p<0.05), significantly faster heart rate (p<0.001) and significantly changed QT and QTc intervals (p<0.001) when compared to the baseline. This model also demonstrated decreased systolic arterial pressure (p<0.01) and increased BNP (p<0.05). The appropriate regression equations were QT = 152.4+0.08RR for normal dogs and QT = 32.3 (RR) 0.29 for HF dogs. In normal dogs, the Van de Water formula reveals the least dependency of QTc on RR interval. In HF dogs, the Fridericia formula appears to be the best equation to remove the influence of heart rate.

Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan

The Thai Journal of Veterinary Medicine

Identification of X or Y chromosome is a very useful technique to verify the sex of boar sperm, but common methods used in pig such as Fluorescence In situ Hybridisation (FISH) and whole semen Polymerase Chain Reaction (PCR) have some limitations. FISH is highly accurate, but time-consuming (>3 days). Whole semen PCR is faster than FISH (3-6 h), but not highly accurate (approximate methods). The objective of this study was to develop a fast and highly accurate protocol to identify sex of boar sperm. In the present study, our team developed an alternative sex identification protocol using single cell …

Endocrine And Metabolic Status Of Dairy Cows During Transition Period, Radojica Djoković, Marko Cincović, Vladimir Kurćubić, Milun Petrović, Miroslav Lalović, Boban Jašović, Zoran Stanimirovic

The Thai Journal of Veterinary Medicine

The objective of this study was to determine blood levels of cortisol, insulin, triiodothyronine (T3), thyroxine (T4), glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate (BHB), triglycerides (TG) and total cholesterol in dairy Holstein cows (n = 40) during the transition period. The test cows were classified into four groups: Group A (n = 10), clinically healthy late-pregnant cows from day 20 to day 10 before calving. Group B (n = 10), clinically healthy late-pregnant cows from day 10 to day 1 before calving. Group C (n = 10), clinically healthy puerperal cows. Group D (n = 10), puerperal ketotic cows. The …

Clinical Effect Of Glucosamine And Chondroitin Contained Nutraceutical On Osteoarthritis In Dogs After Anterior Cruciate Ligament Rupture Surgical Repair, Prach Maihasap, Kumpanart Soontornwipart, Natee Techaarpornkul

The Thai Journal of Veterinary Medicine

Glucosamine and chondroitin sulfates arekey structural components of extracellular fluid in articular cartilage. From previous studies, glucosamine and chondroitin sulfates have several positive effects on cartilage homeostasis and are used in osteoarthritis patients. Although glucosamine and chondroitin sulfates have some positive effects on clinical treatment of osteoarthritis, clinical results still need to be investigated. The objective of this study was to investigate the clinical outcomes on lameness score, radiological score, serum WF6 level and serum HA level of glucosamine and chondroitin sulfates in osteoarthritic dogs, using anterior cruciate ligament rupture patients as study models of osteoarthritis (n=12). The results from …

Isoflurane Concentration When Compared With Intramuscular Morphine During Patellar Luxation Repair In Dogs, Pathompong Thapun, Sumit Durongphongtorn, Marissak Kalpravidh

The Thai Journal of Veterinary Medicine

This clinical trial aimed to assess the intraoperative analgesic effect, based on the required minimum alveolarconcentration (MAC) of isoflurane, of transdermal fentanyl when compared with intramuscular morphine in dogsundergoing patellar luxation repair. Seventeen healthy dogs with grade II or grade III of medial patellar luxationwere randomly assigned to group M or group F, receiving a sham patch or a transdermal fentanyl patch (25 μg/h),respectively, applied on the skin at least 24 h prior to surgery. Three dogs had bilateral medial patellar luxation andwere then enrolled in both groups, resulting in ten animals for each group. Before surgery, group M dogs …