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Prevalence And Antimicrobial Resistance Of Salmonella And Campylobacter Species Isolated From Laying Duck Flocks In Confinement And Free-Grazing Systems, Chalermkiat Saengthongpinit, Siriporn Kongsoi, Srisamai Viriyarampa, Thaweesak Songserm 2015 Chulalongkorn University

Prevalence And Antimicrobial Resistance Of Salmonella And Campylobacter Species Isolated From Laying Duck Flocks In Confinement And Free-Grazing Systems, Chalermkiat Saengthongpinit, Siriporn Kongsoi, Srisamai Viriyarampa, Thaweesak Songserm

The Thai Journal of Veterinary Medicine

Prevalence and antimicrobial resistance of Salmonella and Campylobacter species isolated from duck laying flocks in 7 confinement systems and 7 free-grazing systems were determined in central Thailand. In the confinement system, 13.8% (88/639) of Campylobacter spp. were isolated from cloacal swabs; 72.7% was identified as C. jejuni and 27.3% was identified as C. coli. From drinking and surface water samples, 60.0% of Campylobacter spp. were isolated; all was identified as C. jejuni. In the free-grazing system, 0.3% (2/700) of Campylobacter spp. were isolated from cloacal swabs; all was identified as C. jejuni. In addition, 52.9% was isolated from drinking and …


In Vitro Screening Of Crude Extracts From Thai Plants In The Moraceae And Leguminosae Families On Proliferation Of Swine Lymphocyte And Maturation Of Monocyte-Derived Dendritic Cell, Thaniwan Cheun-Arom, Tullaya Pungchaipat, Dachrit Nilubol, Pornpen Werawatganone, Boonchoo Sritularak, Angkana Tantituvanont 2015 Chulalongkorn University

In Vitro Screening Of Crude Extracts From Thai Plants In The Moraceae And Leguminosae Families On Proliferation Of Swine Lymphocyte And Maturation Of Monocyte-Derived Dendritic Cell, Thaniwan Cheun-Arom, Tullaya Pungchaipat, Dachrit Nilubol, Pornpen Werawatganone, Boonchoo Sritularak, Angkana Tantituvanont

The Thai Journal of Veterinary Medicine

In this study, nine crude extracts from Thai plants in the Moraceae and Leguminosae families were screened for their immunoadjuvant activities on the proliferation of swine lymphocytes and the maturation of monocyte-derived dendritic cells (MoDCs). The study demonstrated that the total phenolic content was significantly higher in the extract from Artocarpus gomezianus (264.17±6.5 mg of gallic acid equivalents/gm of extract) than in the extracts from the other plants. The crude extract from the root bark of A. gomezianus significantly increased the proliferative response of peripheral blood mononuclear cells measured using both MTT and CFSE assays (p < 0.05). In addition, the A. gomezianus extract induced the maturation of MoDCs through major histocompatibility (MHC) Class II and CD80 expression. Constituents of phenolic compounds, in particular tannins and flavonoids in the gamma-benzopyrone nucleus group, may be important for the immunoadjuvant effect. The results of this study suggest that the extract from A. gomezianus have immunoadjuvant activities that may be developed as vaccine adjuvants.


Alteration In Serum Matrix Metalloproteinase-9 Gelatinase Activities In Lymphoma-Bearing Dogs With Chemotherapies, Hsin-Yu Huang, Ju-Ying Hsu, Shih-Chieh Chang, Kuan-Sheng Chen, Tien-Huan Hsu, Hsien-Chi Wang 2015 Chulalongkorn University

Alteration In Serum Matrix Metalloproteinase-9 Gelatinase Activities In Lymphoma-Bearing Dogs With Chemotherapies, Hsin-Yu Huang, Ju-Ying Hsu, Shih-Chieh Chang, Kuan-Sheng Chen, Tien-Huan Hsu, Hsien-Chi Wang

The Thai Journal of Veterinary Medicine

Matrix metalloproteinase-2 and -9 (MMP-2 and -9), gelatinases among MMPs, are associated with cell migration, invasion, metastasis and angiogenesis in human solid tumor. The prognostic value of gelatinases in canine lymphoma remains equivocal. To evaluate the relation between serum gelatinases and canine lymphoma, gelatin zymography was used to detect the levels of serum MMP-2 and MMP-9 gelatinase activities in lymphoma-bearing dogs. In this retrospective study, twenty-four canine patients with different subtypes of lymphoma were identified according to the histopathogical examination, and 10 healthy dogs were included as control. Serum samples from the lymphoma-bearing dogs were collected before and after chemotherapy. …


Efficacy Of Live H120 Strain Against Variant Genotype Infectious Bronchitis Virus Isolated In Thailand In Broiler Chickens, Tawatchai Pohuang, Jiroj Sasipreeyajan 2015 Chulalongkorn University

Efficacy Of Live H120 Strain Against Variant Genotype Infectious Bronchitis Virus Isolated In Thailand In Broiler Chickens, Tawatchai Pohuang, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

This study investigated the level of protection generated by vaccination with a live attenuated infectious bronchitis virus (IBV) vaccine, H120 strain against variant genotype IBV isolated in Thailand. Sixty 1-day-old female broiler chickens were randomly divided into 4 groups of 15 chickens each. The chickens in Group 1 were vaccinated at 1 day old, whereas the chickens in Group 2 were vaccinated at 7 days old. Groups 3 and 4 did not receive IBV vaccine and served as positive and negative control groups, respectively. At 21 days of age, the chickens in Groups 1-3 were individually challenged with 104.8 EID50 …


Determination Of Serum Lactate And Glucose In Dogs During Swimming Exercise, Korakot Nganvongpanit, Parptawan Deein, Sajika See-Ngam, Terdsak Yano, Puntita Siengdee, Siriphun Kongsawasdi 2015 Chulalongkorn University

Determination Of Serum Lactate And Glucose In Dogs During Swimming Exercise, Korakot Nganvongpanit, Parptawan Deein, Sajika See-Ngam, Terdsak Yano, Puntita Siengdee, Siriphun Kongsawasdi

The Thai Journal of Veterinary Medicine

The aims of this study were to investigate the levels of lactate and glucose in dogs during swimming and to determine the maximal lactate steady state in small and large breeds of dog. Twelve healthy dogs, including small breed (n = 6) and large breed (n = 6), were the study subjects. After swimming for different periods (5, 10, 15, 20, 25 and 30 min), blood was collected from the dogs which was then used to analyze changes in lactate and glucose levels during swimming. Results showed that the mean frequency of leg movement of the small breed was 99.7 …


Ophthalmology Snapshot, Nalinee Tuntivanich 2015 Chulalongkorn University

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Development Of An Indirect Elisa, Blocking Elisa, Fluorescent Microsphere Immunoassay And Fluorescent Focus Neutralization Assay For Serologic Evaluation Of Exposure To North American Strains Of Porcine Epidemic Diarrhea Virus, Faten Okda, Xiaodong Liu, Aaron Singrey, Travis Clement, Julie Nelson, Jane Christopher-Hennings, Eric A. Nelson, Steven Lawson 2015 South Dakota State University

Development Of An Indirect Elisa, Blocking Elisa, Fluorescent Microsphere Immunoassay And Fluorescent Focus Neutralization Assay For Serologic Evaluation Of Exposure To North American Strains Of Porcine Epidemic Diarrhea Virus, Faten Okda, Xiaodong Liu, Aaron Singrey, Travis Clement, Julie Nelson, Jane Christopher-Hennings, Eric A. Nelson, Steven Lawson

Veterinary and Biomedical Sciences Faculty Publications

Recent, severe outbreaks of porcine epidemic diarrhea virus (PEDV) in Asia and North America highlight the need for well-validated diagnostic tests for the identification of PEDV infected animals and evaluation of their immune status to this virus. PEDV was first detected in the U.S. in May 2013 and spread rapidly across the country. Some serological assays for PEDV have been previously described, but few were readily available in the U.S. Several U.S. laboratories quickly developed indirect fluorescent antibody (IFA) assays for the detection of antibodies to PEDV in swine serum, indicating prior exposure. However, the IFA has several disadvantages, including …


Variation In Brain Morphology Of Intertidal Gobies: A Comparison Of Methodologies Used To Quantitatively Assess Brain Volumes In Fish, Gemma E. White, Culum Brown 2015 Macquarie University

Variation In Brain Morphology Of Intertidal Gobies: A Comparison Of Methodologies Used To Quantitatively Assess Brain Volumes In Fish, Gemma E. White, Culum Brown

Veterinary Science and Medicine Collection

When correlating brain size and structure with behavioural and environmental characteristics, a range of techniques can be utilised. This study used gobiid fishes to quantitatively compare brain volumes obtained via three different methods; these included the commonly used techniques of histology and approximating brain volume to an idealised ellipsoid, and the recently established technique of X-ray micro-computed tomography (micro-CT). It was found that all three methods differed significantly from one another in their volume estimates for most brain lobes. The ellipsoid method was prone to over- or under-estimation of lobe size, histology caused shrinkage in the telencephalon, and although micro-CT …


Prediction Of Parturition In Holstein Dairy Cattle Using Electronic Data Loggers, M. Titler, M. G. Maquivar, S. Bas, P. J. Rajala-Schultz, E. Gordon, K. McCullough, P. Federico, G. M. Schuenemann 2015 The Ohio State University

Prediction Of Parturition In Holstein Dairy Cattle Using Electronic Data Loggers, M. Titler, M. G. Maquivar, S. Bas, P. J. Rajala-Schultz, E. Gordon, K. Mccullough, P. Federico, G. M. Schuenemann

Physiology Collection

The objective of the present study was to assess the effect of parturition on behavioral activity [steps, standing time, lying time, lying bouts (LB), and duration of LB] 4 d before calving using electronic data loggers. Animals (n = 132) from 3 herds were housed in similar freestall barns using a prepartum pen 21 d before the expected calving date and were moved into a contiguous individual maternity pen for parturition. Electronic data loggers were placed on a hind leg of prepartum heifers (heifers, n = 33) and cows (cows, n = 99) at 7 ± 3 d before the …


The Cestode Community In Northern Fur Seals (Callorhinus Ursinus) On St. Paul Island, Alaska, Tetiana A Kuzmina, Jesús S. Hernández-Orts, Eugene T. Lyons, Terry R Spraker, Vadym V. Kornyushyn, Roman Kuchta 2015 Schmalhausen Institute of Zoology NAS of Ukraine, Ukraine

The Cestode Community In Northern Fur Seals (Callorhinus Ursinus) On St. Paul Island, Alaska, Tetiana A Kuzmina, Jesús S. Hernández-Orts, Eugene T. Lyons, Terry R Spraker, Vadym V. Kornyushyn, Roman Kuchta

Veterinary Science Faculty Publications

The diversity and ecology of cestodes from the northern fur seals, Callorhinus ursinus (NFS), were examined using newly collected material from 756 humanely harvested subadult males between 2011 and 2014. NFSs were collected from five different haul-outs on St. Paul Island, Alaska. A total of 14,660 tapeworms were collected with a prevalence of 98.5% and intensity up to 107 cestodes per host (mean intensity 19.7 ± 16.5 SD). Three species of tapeworms were found: Adenocephalus pacificus (Diphyllobothriidea) was the most prevalent (prevalence 97.4%), followed by Diplogonoporus tetrapterus (49.7%), and 5 immature specimens of Anophryocephalus cf. ochotensis (Tetrabothriidea) (0.5%). Most of …


Beef Cattle Management Systems For Estrus Synchronization And Heifer Development, Hazy Rae Nielson 2015 University of Nebraska-Lincoln

Beef Cattle Management Systems For Estrus Synchronization And Heifer Development, Hazy Rae Nielson

Department of Animal Science: Dissertations, Theses, and Student Research

Four experiments were conducted to evaluate estrus synchronization and heifer development systems. In the first experiment delaying AI 19 h following GnRH administration in a hybrid estrus detection and time AI protocol was evaluated. Final pregnancy rate was not different among heifers detected in estrus, AI at the time GnRH administration, or delayed AI. The second study compared the effect of melengestrol acetate (MGA) -PG and 14-d controlled internal drug release (CIDR) -PG estrus synchronization protocols on estrus response and pregnancy rates of 311 d old heifers. Final pregnancy rate was similar between CIDR and MGA treatment groups. The objective …


Repurposing Celecoxib As A Topical Antimicrobial Agent, Shankar Thangamani, Waleed Younis, Mohamed N. Seleem 2015 Purdue University

Repurposing Celecoxib As A Topical Antimicrobial Agent, Shankar Thangamani, Waleed Younis, Mohamed N. Seleem

Department of Comparative Pathobiology Faculty Publications

There is an urgent need for new antibiotics and alternative strategies to combat multidrug-resistant bacterial pathogens, which are a growing clinical issue. Repurposing existing approved drugs with known pharmacology and toxicology is an alternative strategy to accelerate antimicrobial research and development. In this study, we show that celecoxib, a marketed inhibitor of cyclooxygenase-2, exhibits broad-spectrum antimicrobial activity against Gram-positive pathogens from a variety of genera, including Staphylococcus, Streptococcus, Listeria, Bacillus, and Mycobacterium, but not against Gram-negative pathogens. However, celecoxib is active against all of the Gram-negative bacteria tested, including strains of, Acinetobacter, and Pseudomonas, when …


Diffusion Tensor Imaging Of Dolphin Brains Reveals Direct Auditory Pathway To Temporal Lobe, Gregory S. Berns, Peter F. Cook, Sean Foxley, Saad Jbabdi, Karla L. Miller, Lori Marino 2015 Emory University

Diffusion Tensor Imaging Of Dolphin Brains Reveals Direct Auditory Pathway To Temporal Lobe, Gregory S. Berns, Peter F. Cook, Sean Foxley, Saad Jbabdi, Karla L. Miller, Lori Marino

Veterinary Science and Medicine Collection

The brains of odontocetes (toothed whales) look grossly different from their terrestrial relatives. Because of their adaptation to the aquatic environment and their reliance on echolocation, the odontocetes’ auditory system is both unique and crucial to their survival. Yet, scant data exist about the functional organization of the cetacean auditory system. A predominant hypothesis is that the primary auditory cortex lies in the suprasylvian gyrus along the vertex of the hemispheres, with this position induced by expansion of ‘associative0 regions in lateral and caudal directions. However, the precise location of the auditory cortex and its connections are still unknown. Here, …


Epidemiological Associations Between Brachycephaly And Upper Respiratory Tract Disorders In Dogs Attending Veterinary Practices In England, Dan G. O'Neill, Caitlin Jackson, Jonathan H. Guy, David B. Church, Paul D. McGreevy, Peter Thomson, Dave C. Brodbelt 2015 The Royal Veterinary College

Epidemiological Associations Between Brachycephaly And Upper Respiratory Tract Disorders In Dogs Attending Veterinary Practices In England, Dan G. O'Neill, Caitlin Jackson, Jonathan H. Guy, David B. Church, Paul D. Mcgreevy, Peter Thomson, Dave C. Brodbelt

Epidemiology Collection

Background: Brachycephalic dog breeds are increasingly common. Canine brachycephaly has been associated with upper respiratory tract (URT) disorders but reliable prevalence data remain lacking. Using primary-care veterinary clinical data, this study aimed to report the prevalence and breed-type risk factors for URT disorders in dogs. Results: The sampling frame included 170,812 dogs attending 96 primary-care veterinary clinics participating within the VetCompass Programme. Two hundred dogs were randomly selected from each of three extreme brachycephalic breed types (Bulldog, French Bulldog and Pug) and three common small-to medium sized breed types (moderate brachycephalic: Yorkshire Terrier and non-brachycephalic: Border Terrier and West Highland …


Growing South Dakota (Summer 2015), College of Agriculture &. Biological Sciences 2015 South Dakota State University

Growing South Dakota (Summer 2015), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Exciting New Era Underway: New Facilities & New Degree Offerings Coming To Fruition
[Page] 5 USDA Report Touts Agriculture Among Best Fields For College Grads
[Page] 6 Critical Need: Essential Services Of ADRDL Need Support For Modernized Facility
[Page] 8 Quarterly Colloquium: State's Ag Leaders Collaborate
[Page] 9 Profiles In Leadership: Developing A Museum To Enrich And Transform Lives
[Page] 10 Productive Partnerships: Collaboration Between Producers & Researchers Guides Research Farm Efforts
[Page] 12 Coordinated Effort: SDSU's John Ball Serves Dual Role To Benefit South Dakota's Trees
[Page] 14 Progressive Perspective: Dan Oedekoven Reflects On Extension's Evolution
[Page] …


An Evaluation Of Porcine Epidemic Diarrhea Virus Survival In Individual Feed Ingredients In The Presence Or Absence Of A Liquid Antimicrobial, Scott Dee, Casey Neill, Travis Clement, Aaron Singrey, Jane Christopher-Hennings, Eric A. Nelson 2015 South Dakota State University

An Evaluation Of Porcine Epidemic Diarrhea Virus Survival In Individual Feed Ingredients In The Presence Or Absence Of A Liquid Antimicrobial, Scott Dee, Casey Neill, Travis Clement, Aaron Singrey, Jane Christopher-Hennings, Eric A. Nelson

Veterinary and Biomedical Sciences Faculty Publications

Background: Contaminated complete feed and porcine plasma are risk factors for PEDV introduction to farms and a liquid antimicrobial has been proven useful for reducing risk. This study provides information on the survivability of PEDV across common swine feed ingredients in the presence or absence of the liquid antimicrobial.
Results: Eighteen ingredients commonly included in commercial swine diets were selected, including 3 grain sources (corn, soybean meal (SBM), dried distillers grains with solubles (DDGS)), 5 porcine by-products (spray-dried plasma, purified plasma, intestinal mucosa, meat and bone meal and red blood cells (RBCs)), 3 vitamin/trace mineral (VTM) mixes (sow, nursery, finishing), …


Changes In Serum Strongylus Vulgaris-Specific Antibody Concentrations In Response To Anthelmintic Treatment Of Experimentally Infected Foals, Martin Krarup Nielsen, Jessica Scare, Holli Sullivan Gravatte, Jennifer Lynn Bellaw, Julio C. Prado, Craig Robert Reinemeyer 2015 University of Kentucky

Changes In Serum Strongylus Vulgaris-Specific Antibody Concentrations In Response To Anthelmintic Treatment Of Experimentally Infected Foals, Martin Krarup Nielsen, Jessica Scare, Holli Sullivan Gravatte, Jennifer Lynn Bellaw, Julio C. Prado, Craig Robert Reinemeyer

Maxwell H. Gluck Equine Research Center Faculty Publications

Strongylus vulgaris is the most pathogenic nematode parasite of horses. Its extensive migration in the mesenteric blood vessels can lead to life-threatening intestinal infarctions. Recent work has shown that this parasite is still identified among managed horse populations. A serum enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of migrating larvae of S. vulgaris. Previous work has documented an increase in ELISA values following larvicidal treatment with ivermectin and suggested that the target parasite antigen is primarily produced by the later larval stages. The aim of this study was to experimentally inoculate cohorts of foals with S. …


Comparison Of The Equine Reference Sequence With Its Sanger Source Data And New Illumina Reads, Jovan Rebolledo-Mendez, Matthew S. Hestand, Stephen J. Coleman, Zheng Zeng, Ludovic Orlando, James N. Macleod, Ted Kalbfleisch 2015 University of Louisville

Comparison Of The Equine Reference Sequence With Its Sanger Source Data And New Illumina Reads, Jovan Rebolledo-Mendez, Matthew S. Hestand, Stephen J. Coleman, Zheng Zeng, Ludovic Orlando, James N. Macleod, Ted Kalbfleisch

Maxwell H. Gluck Equine Research Center Faculty Publications

The reference assembly for the domestic horse, EquCab2, published in 2009, was built using approximately 30 million Sanger reads from a Thoroughbred mare named Twilight. Contiguity in the assembly was facilitated using nearly 315 thousand BAC end sequences from Twilight's half brother Bravo. Since then, it has served as the foundation for many genome-wide analyses that include not only the modern horse, but ancient horses and other equid species as well. As data mapped to this reference has accumulated, consistent variation between mapped datasets and the reference, in terms of regions with no read coverage, single nucleotide variants, and small …


Annotation Of The Protein Coding Regions Of The Equine Genome, Matthew S. Hestand, Theodore S. Kalbfleisch, Stephen J. Coleman, Zheng Zeng, Jinze Liu, Ludovic Orlando, James N. MacLeod 2015 University of Kentucky

Annotation Of The Protein Coding Regions Of The Equine Genome, Matthew S. Hestand, Theodore S. Kalbfleisch, Stephen J. Coleman, Zheng Zeng, Jinze Liu, Ludovic Orlando, James N. Macleod

Veterinary Science Faculty Publications

Current gene annotation of the horse genome is largely derived from in silico predictions and cross-species alignments. Only a small number of genes are annotated based on equine EST and mRNA sequences. To expand the number of equine genes annotated from equine experimental evidence, we sequenced mRNA from a pool of forty-three different tissues. From these, we derived the structures of 68,594 transcripts. In addition, we identified 301,829 positions with SNPs or small indels within these transcripts relative to EquCab2. Interestingly, 780 variants extend the open reading frame of the transcript and appear to be small errors in the equine …


In Vitro And In Vivo Evaluation Of Infestation Deterrents Against Lice, Kyong-Sup Yoon, Jennifer K. Ketzis, Samuel W. Andrewes, Christopher S. Wu, Kris Honraet, Dorien Staljanssens, Bart Rossel, J Marshall Clark 2015 Southern Illinois University Edwardsville

In Vitro And In Vivo Evaluation Of Infestation Deterrents Against Lice, Kyong-Sup Yoon, Jennifer K. Ketzis, Samuel W. Andrewes, Christopher S. Wu, Kris Honraet, Dorien Staljanssens, Bart Rossel, J Marshall Clark

SIUE Faculty Research, Scholarship, and Creative Activity

The human head louse is a cosmopolitan ectoparasite and frequently infests many people, particularly school-age children. Due to widespread pyrethroid resistance and the lack of efficient resistance management, there has been a considerable interest in the protection of uninfested people and prevention of reinfestation by disrupting lice transfer. In this study, two nonclinical model systems (in vitro and in vivo) were used to determine the efficacy of the infestation deterrents, Elimax lotion and Elimax shampoo, against human head lice or poultry chewing lice, respectively. With in vitro assessments, female head lice exhibited significantly higher avoidance responses to hair tufts treated …


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