Relationship Between Heat-Labile
Enterotoxin Secretion Capacity And Virulence
In Wild Type Porcine-Origin Enterotoxigenic
Escherichia Coli Strains,
2015
University of Nebraska-Lincoln
Relationship Between Heat-Labile Enterotoxin Secretion Capacity And Virulence In Wild Type Porcine-Origin Enterotoxigenic Escherichia Coli Strains, Prageeth Wijemanne, Jun Xing, Emil M. Berberov, David B. Marx, David H. Francis, Rodney A. Moxley
School of Veterinary and Biomedical Sciences: Faculty Publications
Heat-labile enterotoxin (LT) is an important virulence factor secreted by some strains of enterotoxigenic Escherichia coli (ETEC). The prototypic human-origin strain H10407 secretes LT via a type II secretion system(T2SS). We sought to determine the relationship between the capacity to secrete LT and virulence in porcine-origin wild type (WT) ETEC strains. Sixteen WT ETEC strains isolated from cases of severe diarrheal disease were analyzed by GM1ganglioside enzyme- linked immunosorbent assay tomeasure LT concentrations in culture supernatants. All strains had detectable LT in supernatants by 2 h of culture and 1 strain, which was particularly virulent in gnotobiotic piglets (3030-2), had …
Isolation And Characterization Of Potential Probiotic Bacteria Suitable For Mollusk Larvae Cultures,
2015
Chulalongkorn University
Isolation And Characterization Of Potential Probiotic Bacteria Suitable For Mollusk Larvae Cultures, Ruth Escamilla-Montes, Antonio Luna-González, Ma. Del Carmen Flores-Miranda, Píndaro Álvarez-Ruiz, Jesús Arturo Fierro-Coronado, Ana Claudia Sánchez-Ortiz, José Ávila-Leal
The Thai Journal of Veterinary Medicine
Guts of Crassostrea gigas, C. corteziensis, and Atrina maura were screened for bacilli and lactic acid bacteria with probiotic properties for bivalve mollusks. Characterization of the isolates included Gram stain, morphology, cell arrangement, catalase and hemolytic activity, hydrophobicity, autoaggregation, coaggregation against Vibrio sinaloensis VHPC23, biofilm formation, counting of colony forming units (CFU), NaCl and pH tolerance, enzymatic activity, resistance to antibiotics, antagonism against Vibrio sinaloensis VHPC23, and molecular identification. Three isolates (BCR 4-3, BALCR 5-2, and BALOR 2-3) showed no hemolytic activity, high tolerance to pH and salinity, high adhesion potential, high autoaggregation and coaggregation, biofilm formation, well growth, and …
In Vitro Suppression Against Streptococcal Bacteria And Health-Promoting Effects Of Probiotic Bacillus Polyfermenticus In Tilapia (Oreochromis Niloticus),
2015
Chulalongkorn University
In Vitro Suppression Against Streptococcal Bacteria And Health-Promoting Effects Of Probiotic Bacillus Polyfermenticus In Tilapia (Oreochromis Niloticus), Mintra Lukkana, Sasibha Jantrakajorn, Janenuj Wongtavatchai
The Thai Journal of Veterinary Medicine
In vitro antibacterial activity of Bacillus polyfermenticus (BP) against tilapia streptococcal pathogen was evaluated using the agar dilution method and co-incubation method. Both in vitro tests confirmed the inhibitory effect of B. polyfermenticus on the growth of Streptococcus agalactiae. The suppression of S. agalactiae was observed in the test system containing B. polyfermenticus and the inhibition was more evident in the system with high density of B. polyfermenticus. Subsequently, feeding administration of BP was employed in tilapia broodstocks (12 months old) and fry (37.33 ± 1.37 mg body weight) to examine the effect of BP on health performance. Significantly higher …
Intra-Articular Injections With Allogeneic Dental Pulp Stem Cells For Chronic Osteoarthritis,
2015
Chulalongkorn University
Intra-Articular Injections With Allogeneic Dental Pulp Stem Cells For Chronic Osteoarthritis, Ratikorn Bootcha, Jedee Temwichitr, Soontaree Petchdee
The Thai Journal of Veterinary Medicine
Stem cell therapies are rapidly gaining attention as a promising tool for tissue regeneration. A variety of stem cell types have been studied as potential candidates to restore the structure and function of damaged organs or tissues. Recent studies have demonstrated the use of stem cells isolated from dental pulp in dentistry and tissue engineering. In our previous studies we have shown the applications of puppy deciduous teeth stem cells (pDSCs) to restore infarcted myocardium and ulceration of the cornea. For this study, our aim was to provide a novel therapeutic application of allogeneic pDSCs in dogs with chronic osteoarthritis …
Ecg Quiz,
2015
Chulalongkorn University
Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram
The Thai Journal of Veterinary Medicine
No abstract provided.
What Is Your Diagnosis?,
2015
Chulalongkorn University
What Is Your Diagnosis?, Nan Choisunirachon
The Thai Journal of Veterinary Medicine
No abstract provided.
Morphology Of The Heart Of A White-Cheeked Gibbon (Hylobates Concolor),
2015
Chulalongkorn University
Morphology Of The Heart Of A White-Cheeked Gibbon (Hylobates Concolor), Tilladit Rung-Ruangkijkrai, Wuthichai Klomkleaw, Pakorn Prachammuang
The Thai Journal of Veterinary Medicine
The heart was recovered at post mortem from an adult white-cheeked gibbon, Hylobates concolor, for anatomical and histological studies. Macroscopically, it had oval shape, blunt and spherical apex with 3.5 cm. and 5 cm. in width and length. Smooth edged comma-shaped right auricle was markedly bigger than the jagged- edged left auricle. Pectinate muscles were found both at the internal surface of the right auricle and lateral free wall of the right atrium, while limited only in blind part of the left auricle. The coronary arteries supply was left coronary type. The left coronary artery gave circumflex and paraconal interventricular …
Environmental Microbes And Uveitis: Is Microbial
Exposure Always Bad?,
2015
University of Nebraska-Lincoln
Environmental Microbes And Uveitis: Is Microbial Exposure Always Bad?, Chandirasegaran Massilamany, A. Gangaplara, J. Reddy
School of Veterinary and Biomedical Sciences: Faculty Publications
The eye generally is considered to be an immune-privileged organ, but this notion is being increasingly challenged as ocular antigens can be expressed in the generative lymphoid organs, resulting in attainment of self-tolerance. What triggers a break in this tolerant state is a fundamental question in autoimmunity research. The general belief is that exposure to environmental microbes can break self-tolerance in genetically susceptible individuals, leading to the induction of autoimmune responses. The molecular mimicry hypothesis has been proposed as one major mechanistic, pathway through which microbes, by generating crossreactive immune responses, can induce ocular damage of the kind that might …
Effects Of Immunization Against Gonadotropin Releasing Hormone On Reproductive Functions In Male Rusa Deer (Rusa Timorensis),
2015
Chulalongkorn University
Effects Of Immunization Against Gonadotropin Releasing Hormone On Reproductive Functions In Male Rusa Deer (Rusa Timorensis), Orasa Phraluk, Worawidh Wajjwalku, Boripat Siriaroonrat, Orawan Booddee, Nikorn Thongtip
The Thai Journal of Veterinary Medicine
We performed an evaluation of the effect of gonadotropin releasing hormone (GnRH) vaccine on male rusa deer reproductive function. Mature male rusa deer (Rusa timorensis) were divided into two groups: untreated group (n=3) and treated group (n=7). The treated rusa deer were administered with two ml of the GnRH-protein conjugate vaccine (Improvac®; 200 μg/ml). The vaccine was injected subcutaneously into the treated group three times at four-week intervals (April, May and June) and an additional single dose booster was given after a decrease in GnRH antibody levels was detected (October). Blood testosterone concentrations and GnRH antibody titer were determined before …
Ultrasound Diagnosis,
2015
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Optimization Of Cell Permeabilization For Rapid Detection Of Salmonella In Pork By Fish,
2015
Chulalongkorn University
Optimization Of Cell Permeabilization For Rapid Detection Of Salmonella In Pork By Fish, Damrongsak Arlai, Sujate Chuanchom, Theerapol Sirinarumitr
The Thai Journal of Veterinary Medicine
Salmonella spp. are one of the major causes of infectious gastroenteritis. The route of infection is usually ingestion of contaminated food. Due to the severity of the disease, it is worthwhile to identify appropriate preventive measures. Bacterial culture method is the gold standard method, but it is time-consuming (at least 4-6 days). Therefore, in this research, the fluorescence in situ hybridization technique (FISH) was optimized for rapid and accurate detection of Salmonella spp. in contaminated pork using a 3¢-end tailing oligonucleotide probe. The optimum digestion condition for cell permeation with lysozyme was 1 mg/ml at 37oC for 3 min. The …
Antibiotic Resistance And Molecular Characterization Of Enterobacter Cancerogenus Isolated From Wild Birds In Taif Province, Saudi Arabia,
2015
Chulalongkorn University
Antibiotic Resistance And Molecular Characterization Of Enterobacter Cancerogenus Isolated From Wild Birds In Taif Province, Saudi Arabia, Aly E. Abo-Amer, Mohammed Y. Shobrak
The Thai Journal of Veterinary Medicine
Enterobacter infection can cause diseases such as bacteremia, lower respiratory tract infections, skin and soft-tissue infections, urinary tract infections, etc. Enterobacter could be isolated from natural environments. Enterobacter infections can require prolonged hospitalization and powerful and expensive antimicrobial agents. Increase in the number of resistant and multi-resistant strains of bacteria is a major concern to health officials worldwide. Therefore, the objective of the study was to evaluate the prevalence of antibiotic-resistant Enterobacter sp. recovered from wild birds collected in Taif province, Saudi Arabia. Fifty isolates were recovered from cloacal samples of resident wild birds and 32 isolates from cloacal samples …
Effect Of Activated Charcoal-Supplemented Diet On Growth Performance And Intestinal Morphology Of Nile Tilapia (Oreochromis Niloticus),
2015
Chulalongkorn University
Effect Of Activated Charcoal-Supplemented Diet On Growth Performance And Intestinal Morphology Of Nile Tilapia (Oreochromis Niloticus), Nopadon Pirarat, Surinton Boonananthanasarn, Laddawan Krongpong, Takayuki Katagiri, Masashi Maita
The Thai Journal of Veterinary Medicine
The effect of tilapia diet supplemented with activated charcoal (AC) on growth performance and intestinal morphology of Nile Tilapia was examined. The fish were divided into four groups: a control group and three AC-supplementation groups (1%, 2% and 3% of AC in diet), and fed approximately 3% of body weight twice a day for 30 days. Growth performance and intestinal villus height were measured. Results revealed that the 2% AC-supplemented diet group was significantly (P<0.05) better in terms of percentage weight gain, specific growth rate (SGR) and feed conversion ratio (FCR), among the experimental groups. The foregut and midgut villus height of the 3% and 2% AC-supplemented diet groups was similar, but was significantly higher than the control group (P<0.05). Consequently, the supplementation of diet with 2% AC was found to be the most suitable for improving the growth performance and intestinal morphology in tilapia.
Disseminated Oral Melanoma With Brain And Gastrointestinal Tract Involvements In A Cocker Spaniel: A Case Report,
2015
Chulalongkorn University
Disseminated Oral Melanoma With Brain And Gastrointestinal Tract Involvements In A Cocker Spaniel: A Case Report, Sawang Kesdangsakonwut, Katriya Chankow, Anudep Rungsipipat
The Thai Journal of Veterinary Medicine
A nine-year-old, male Cocker Spaniel with a history of surgical excision of oral melanoma located at the gingiva of the left maxilla was evaluated progressively over the course of 1 month post-surgery. The dog had shown signs of respiratory distress. Radiographic examination revealed diffusely pulmonary metastases. The respiratory distress progressively worsened and the dog died following excision of the oral mass. On necropsy, disseminated metastases to various organs including the lungs, heart, mediastinum, liver, kidneys, spleen, tonsil, lymph nodes, adrenal glands, thoracic wall, jejunum, pancreas, and brain were observed. Microscopically, the neoplastic cells were characterized by spindle to polyhedral phenotypes, …
Ophthalmology Snapshot,
2015
Chulalongkorn University
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
Isolation And Molecular Characterization Of Multidrug-Resistant Salmonella, Shigella And Proteus From Domestic Birds,
2015
Chulalongkorn University
Isolation And Molecular Characterization Of Multidrug-Resistant Salmonella, Shigella And Proteus From Domestic Birds, Aly E. Abo-Amer, Mohammed Y. Shobrak
The Thai Journal of Veterinary Medicine
The increase in multidrug-resistant enteric bacteria, especially Salmonella, Shigella and Proteus in poultry has become a significant public health threat. Therefore, this work was aimed to investigate whether domestic birds have a role in the emergence and transmission of multidrug-resistant (MDR) bacteria in Taif province. Salmonella, Shigella and Proteus were isolated from different types of healthy domestic birds (n=42) collected from different places. Bacterial isolates were recovered from cloacal swabs of birds by non-selective and selective pre-enrichment technique. One hundred and sixty six bacterial isolates were screened for antibiotic susceptibility such as Cefaclor, Oxacillin, Ampicillin, Chloramphenicol, Cephalexin, Neomycin, Colistin, Ciprofloxacin, …
Comparison Of Two Locations For Recording Intracardiac Conductivity In Anesthetized Dogs,
2015
Chulalongkorn University
Comparison Of Two Locations For Recording Intracardiac Conductivity In Anesthetized Dogs, Nakkawee Saengklub, Vudhiporn Limprasutr, Suwanakiet Sawangkoon, Chollada Buranakarl, Narongsak Chaiyabutr, Anusak Kijtawornrat
The Thai Journal of Veterinary Medicine
In safety pharmacological and toxicological studies, all new chemical entities should be investigated for the possibility of slow atrioventricular (AV) conduction especially at the infranodal of AV node. The objective of this study was to compare two locations of His bundle electrogram (HBE) recording, the right side (right atrium) and the left side (non-coronary cusp), of the heart in anesthetized dogs. Diltiazem was used to evaluate these two locations. The dogs (n=6) were anesthetized with morphine and a-chloralose. In order to record HBE, two catheters with multiple electrodes were placed in the right atrium via jugular vein and in the …
Antimicrobial Use And Multidrug-Resistant Salmonella Spp., Escherichia Coli, And Enterococcus Faecalis In Swine From Northern Thailand,
2015
Chulalongkorn University
Antimicrobial Use And Multidrug-Resistant Salmonella Spp., Escherichia Coli, And Enterococcus Faecalis In Swine From Northern Thailand, David C. Love, Prasit Tharavichitkul, Orapun Arjkumpa, Maho Imanishi, Soawapak Hinjoy, Kenrad Nelson, Keeve E. Nachman
The Thai Journal of Veterinary Medicine
The aim of this study was to isolate antimicrobial-resistant bacteria from intensive commercial and backyard swine operations (farms) in Northern Thailand and to characterize antimicrobial usage among swine farmers. A total of 51 swine farms in Chiang Mai, Lampoon, and Nan provinces were visited from February to October 2011 to collect swine fecal samples for bacterial isolation (Salmonella spp., Escherichia coli, Enterococcus faecalis) and antimicrobial resistance testing. A survey about production intensity, management practices, animal health, and antimicrobial usage was conducted with a sub-set of farms (n = 20) located in Nan province. The farm-level prevalence of drug-resistant E. coli, …
An Inactivated Vaccine For Prevention And Control Of Inclusion Body Hepatitis In Broiler Breeders,
2015
Chulalongkorn University
An Inactivated Vaccine For Prevention And Control Of Inclusion Body Hepatitis In Broiler Breeders, Sucheeva Junnu, Preeda Lertwatcharasarakul, Siriluk Jala, Sakuna Phattanakulanan, Angkasiya Monkong, Sittinee Kulprasertsri, Chotiga Thivalai, Warunya Chakritbudsabong, Kridsada Chaichoun, Thaweesak Songserm
The Thai Journal of Veterinary Medicine
Inclusion bodies hepatitis (IBH) in Thailand is caused by Fowl Adenovirus (FAdv) serotype 2. Vertical transmission is the important route for disease outbreak. Biosecurity and vaccination are the major keys for disease control and prevention. Vaccination of broiler breeders results in maternal antibody production that can be transferred to their progenies. Therefore, the aim of present study was to develop an inactivated FAdv serotype 2 vaccine. The FAdv was isolated from liver of infected chickens by inoculation onto chicken embryo liver cells (CEL). Virus purification was performed and then inactivated by Binary Ethylene Imine (BEI). The 107 TCID50 of virus …
In Vitro Modulatory Effects Of Quercetin On Bovine Neutrophil Effector Functions,
2015
Chulalongkorn University
In Vitro Modulatory Effects Of Quercetin On Bovine Neutrophil Effector Functions, Phongsakorn Chuammitri, Chiradet Amphaiphan, Panupong Nojit
The Thai Journal of Veterinary Medicine
Bovine neutrophils perform numerous effector functions to overcome microbial invasions by utilizing innately-equipped intracellular and extracellular killing mechanisms. In this study, we explored the modulatory effects of quercetin hydrate (QH), an herbal flavonoid compound, on bovine neutrophil functional activities. Isolated neutrophils were incubated with various concentrations of QH (0-100 mM). Neutrophil viability results showed no signs of cytotoxicity (p = 0.33). In other assays, neutrophils were stimulated with a pre-defined concentration of QH (50 mM). Results showed promising effects of QH on enhancing intracellular ROS generation (p < 0.0001) compared to control. Phagocytic activity of fluorescently labeled E.coli was also augmented by the effects of this compound (p = 0.05). However, intracellular killing of live E.coli quantified by MTT assay demonstrated a slight decrease in activity (p = 0.304). Additionally, extracellular killing driven by NET-DNA release was remarkably higher in QH-stimulated cells (p = 0.0004). Supplementation of QH to LPS stimulated neutrophils resulted in significantly decreased pro-inflammatory cytokines, IL-1b (p < 0.0001) and TNF-a gene expression (p = 0.004). Taken together, quercetin could exert its noteworthy actions, both at cellular and molecular levels, at a certain physiological concentration.
