Trim21 Regulates Nmi-Ifi35 Complex-Mediated Inhibition
Of Innate Antiviral Response,
2015
University of Nebraska–Lincoln
Trim21 Regulates Nmi-Ifi35 Complex-Mediated Inhibition Of Innate Antiviral Response, Anshuman Das, Phat X. Dinh, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
In this study, using an immunoprecipitation coupled with mass spectrometry approach, we have identified the E3 ubiquitin ligase Trim21 as an interacting partner of IFI35 and Nmi. We found that this interaction leads to K63-linked ubiquitination on K22 residue of Nmi, but not IFI35. Using domain deletion analysis, we found that the interaction is mediated via the coiled-coil domain of Nmi and the carboxyl-terminal SPRY domain of Trim21. Furthermore, we show that depletion of Trim21 leads to significantly reduced interaction of Nmi with IFI35, which results in the abrogation of the negative regulatory function of the Nmi-IFI35 complex on innate …
Complete Genome Sequence Of Noncytopathic Bovine Viral Diarrhea Virus 1 Contaminating A High-Passage Rk-13 Cell Line,
2015
University of Kentucky
Complete Genome Sequence Of Noncytopathic Bovine Viral Diarrhea Virus 1 Contaminating A High-Passage Rk-13 Cell Line, Bora Nam, Ganwu Li, Ying Zheng, Jianqiang Zhang, Kathleen M. Shuck, Peter J. Timoney, Udeni B. R. Balasuriya
Maxwell H. Gluck Equine Research Center Faculty Publications
A high-passage rabbit kidney RK-13 cell line (HP-RK-13[KY], originally derived from the ATCC CCL-37 cell line) used in certain laboratories worldwide is contaminated with noncytopathic bovine viral diarrhea virus (ncpBVDV). On complete genome sequence analysis, the virus strain was found to belong to BVDV group 1b.
Recurrent Streptococcus Equi Subsp. Zooepidemicus Bacteremia In An Infant,
2015
Ohio State University
Recurrent Streptococcus Equi Subsp. Zooepidemicus Bacteremia In An Infant, Joshua R. Watson, Amy Leber, Sridhar Velineni, John F. Timoney, Monica I. Ardura
Maxwell H. Gluck Equine Research Center Faculty Publications
We describe a case of an infant with recurrent bacteremia caused by Streptococcus equi subsp. zooepidemicus, likely transmitted from mother to infant. Our case highlights the importance of an epidemiological history and molecular diagnostics in ascertaining insights into transmission, pathogenesis, and optimal management.
Low-Dose Cyclophosphamide And Piroxicam Inhibit Growth, Migration, And Invasion Of Canine Oral Malignant Melanoma Cell Line,
2015
Chulalongkorn University
Low-Dose Cyclophosphamide And Piroxicam Inhibit Growth, Migration, And Invasion Of Canine Oral Malignant Melanoma Cell Line, Nan Choisunirachon, Kota Yoshida, Kohei Saeki, Yuiko Tanaka, Manabu Mochizuki, Ryohei Nishimura, Nobuo Sasaki, Takayuki Nakagawa
The Thai Journal of Veterinary Medicine
The effect of low-dose chemotherapy on tumor motility of canine oral malignant melanoma has been investigated on an in vitro model using low-dose cyclophosphamide and piroxicam. Cyclophosphamide, at a concentration of 0.1 mg/mL in culture medium, significantly inhibited tumor cell migration/invasion through trans-well assays and suppressed both ERK and NF-κB signaling, the latter of which was observed through IκB-α and P-IκBα expression. Furthermore, in combination with a COX-2 inhibitor, i.e. piroxicam (0.38 mg/mL in culture medium), cyclophosphamide revealed a potential anti-migration effect over the single piroxicam treatment on a wound assay. This result suggested that low-dose cyclophosphamide might have influence …
Prevalence Of Porcine Sapovirus In Suckling Piglets In Hunan Province, China,
2015
Chulalongkorn University
Prevalence Of Porcine Sapovirus In Suckling Piglets In Hunan Province, China, Sheng-Guo Tan, Na Liu, Ping-Yuan Xu
The Thai Journal of Veterinary Medicine
Sapoviruses (SaVs) are emerging enteric pathogens that cause diarrhea in humans and animals. In the current study, genetic identification of sapovirus (SaV) in suckling piglets was investigated in Hunan province, China between May 2013 and May 2014. Diarrheic fecal (n=300) samples collected from suckling piglets in Hunan province, China were evaluated using RT-PCR and then sequenced. Overall, 45 of the 300 (15%) samples were found to contain porcine SaV. In addition, phylogenetic analysis showed that all the porcine SaV isolates belonged to the porcine SaV genogroup III (GIII). The results of the present investigation have implications for the control of …
Case Report: Efficacy Of Combination Of Electroacupuncture And Aquapuncture Using Vitamin B Complex On Promotion Of Ambulation Perception In 15 Dogs With Hansen Type I Intervertebral Disc Disease Undergoing Hemilaminectomy,
2015
Chulalongkorn University
Case Report: Efficacy Of Combination Of Electroacupuncture And Aquapuncture Using Vitamin B Complex On Promotion Of Ambulation Perception In 15 Dogs With Hansen Type I Intervertebral Disc Disease Undergoing Hemilaminectomy, Yu-Chun Cheng, Jian-Liang Lin, Sophia Hsiao-Ching Su, Pin-Cheng Shih, Kuan-Sheng Chen, Hsien-Chi Wang, Wei-Ming Lee
The Thai Journal of Veterinary Medicine
Fifteen Dachshunds, 6 females and 9 males, aged from 3 to 7.1 years old and weighing from 8.9 to 18.4 kg, were presented with various degrees of paralysis with deep pain on both limbs. The existence of clinical signs was observed by the owners and ranged from 1 to over 7 days. These dogs showed grades of dysfunction ranging from 2 to 5 depending on neurological examination. Hansen type I IVDD was diagnosed in these dogs based on the neurological and imaging examination. Hemilaminectomy was performed on these dogs. Electroacupuncture (using 15-40 Hz on acupoints hua-tuo-jia-ji, GV-14, Bai-hui, KID-1 for …
Ecg Quiz,
2015
Chulalongkorn University
Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram, Nawat Sannamwong
The Thai Journal of Veterinary Medicine
No abstract provided.
Ultrasound Diagnosis,
2015
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Study Of Free Radical Scavenging Activity Of Antibiotic Growth Promoters Flavophospholipol And Avilamycin,
2015
Chulalongkorn University
Study Of Free Radical Scavenging Activity Of Antibiotic Growth Promoters Flavophospholipol And Avilamycin, Krittika Kabploy, Nuntavan Bunyapraphatsara, Noppawan Phumala Morales, Nuanchan Paraksa
The Thai Journal of Veterinary Medicine
Non-antibiotic feed additives have been developed to be used as alternatives, but none of them is effective as antibiotic growth promoters (AGPs). Understanding other mechanisms related to growth promoter of AGPs may be beneficial to the search for effective feed additives for animal production. In vitro and in vivo studies were conducted to evaluate the free radical scavenging activities of flavophospholipol and avilamycin. The in vitro study showed that flavophospholipol had a potent scavenging activity for DPPH, hydroxyl and nitric oxide radicals with the exception of superoxide anion radicals and their IC50 were 155.1, 62.6 and 105.6 mg/l, respectively. The …
Comparison Of Systolic Blood Pressure Between Normal And Asymptomatic Degenerative Mitral Valve Diseased In Small Breed Dogs,
2015
Chulalongkorn University
Comparison Of Systolic Blood Pressure Between Normal And Asymptomatic Degenerative Mitral Valve Diseased In Small Breed Dogs, Sirilak Disatian Surachetpong, Kamonwan Pradit, Panyarat Chongsawat, Salinrat Titada, Sasita Suwantarat
The Thai Journal of Veterinary Medicine
Degenerative mitral valve disease (DMVD) is the major cause of mitral valve regurgitation and heart failure in dogs. The regurgitation of mitral valve may affect blood pressure without clinical presentation. The aims of this study were to compare the systolic blood pressure between normal dogs and dogs affected with asymptomatic DMVD (class B ACVIM classification) and to determine the relationship between echocardiographic values and blood pressure levels. The systolic blood pressure of small breed dogs, weighing less than 10 kilogram, that were normal (n=22) and newly diagnosed with DMVD (n=21) was measured. The average of systolic blood pressure determined by …
Comparison Of The Efficacy Of Firocoxib And Carprofen In Clinical Use For Canine Coxofemoral Osteoarthritis,
2015
Chulalongkorn University
Comparison Of The Efficacy Of Firocoxib And Carprofen In Clinical Use For Canine Coxofemoral Osteoarthritis, Nattawan Tangmahakul, Kumpanart Soontornvipart, Korakot Nganvongpanit
The Thai Journal of Veterinary Medicine
Firocoxib and carprofen, non-steroidal anti-inflammatory drugs (NSAIDs), are used in the treatment of canine osteoarthritis. This study evaluated the efficacy and provided information of these NSAIDs in clinical practice. Sixteen healthy dogs of large breeds over 5 years old without systemic diseases and pregnancy that had coxofemoral osteoarthritis were divided into 2 groups, firocoxib group (n=9) and carprofen group (n=7). The study was conducted for 16 weeks. Firocoxib (5mg/kg body weight) or carprofen (4.4mg/kg body weight) was administered to all dogs once daily for 2 weeks, on alternate days for 6 weeks and every 2 days for 8 weeks. Of …
Study Of Morphological Characteristic And Prevalence Of Haemoproteus Blood Parasite In Passerines In Bung Boraphet,
2015
Chulalongkorn University
Study Of Morphological Characteristic And Prevalence Of Haemoproteus Blood Parasite In Passerines In Bung Boraphet, Phirom Prompiram, Smarn Kaewviyudth, Yaowalark Sukthana, Parntep Rattanakorn
The Thai Journal of Veterinary Medicine
Haemoproteus is one of the world’s widespread avian blood parasite. However, it is rarely reported in Thailand; even at Bung Boraphet, which is an important wetland bird area located in the central part of Thailand, it still has never been reported. Therefore, information on species of haemoproteid and their epidemiology is important for the health of wildlife in Thailand. Samples of blood smear were collected from 633 passerines consisting of 6 orders, 15 families, 25 genera, and 35 species in total from Bung Boraphet. This is the first study to report 8 haemoproteid blood parasite species including Haemoproteus herdiadis, H. …
Genomic Characterization Of A New Tembusu Flavivirus From Domestic Ducks In Thailand,
2015
Chulalongkorn University
Genomic Characterization Of A New Tembusu Flavivirus From Domestic Ducks In Thailand, Warunya Chakritbudsabong, Jarupha Taowan, Preeda Lertwatcharasarakul, Sakuna Phattanakunanan, Angkasiya Munkhong, Thaweesak Songserm, Kridsada Chaichoun
The Thai Journal of Veterinary Medicine
Tembusu virus (TMUV) namely KPS54A61 has spread in duck farms in many provinces in Thailand since 2010. The morbidity ranges from 15-30% and the mortality ranges from 10-15%. All infected ducks exhibit nervous signs including ataxia and paralysis. TMUV was successfully isolated from the brain and spinal cord of infected ducks by using C6/36 and Vero cells, but the typical cytopathic effect (CPE) was presented in the Vero cells only. Phylogenetic analysis of the whole genomic sequence of our TMUV-KPS54A61 virus showed that it belonged to the Ntaya virus group of Flaviviridae and its genomic sequence was similar to that …
Efficacy Of Intraarticular Administration Of Hyaluronic Acid In Osteoarthritis After Surgical Correction Of Canine Cranial Cruciate Ligament Rupture,
2015
Chulalongkorn University
Efficacy Of Intraarticular Administration Of Hyaluronic Acid In Osteoarthritis After Surgical Correction Of Canine Cranial Cruciate Ligament Rupture, Natee Techaarpornkul, Kumpanart Soontornvipart, Prach Maihasap
The Thai Journal of Veterinary Medicine
Canine osteoarthritis is a common disease in clinical practice. Causes of canine osteoarthritis are obscure. At present, nutraceuticals and hyaluronic acid are used for the treatment of canine osteoarthritis. This study assessed the efficacy of intraarticular administration of hyaluronic acid on the treatment of canine osteoarthritis. After undergoing surgical correction of cranial cruciate ligament rupture, 16 dogs were divided into 2 groups, HA and control groups. The dogs in the HA group (n=8) received intraarticular injection of hyaluronic acid. The control group (n=8) did not receive hyaluronic acid treatment after surgery. Lameness score, weight bearing score, radiographic examination and serum …
Efficacy Of Scalp Electroencephalography (Eeg) As A Supportive Diagnostic Tool In Canine Epilepsy Using Dexmedetomidine,
2015
Chulalongkorn University
Efficacy Of Scalp Electroencephalography (Eeg) As A Supportive Diagnostic Tool In Canine Epilepsy Using Dexmedetomidine, Pichayaporn Soison, Waraporn Aumarm, Bordin Tiraphut, Chalermpol Lekcharoensuk, Apasri Lusawat, Kanjana Unnwongse, Khwanrat Wangphonphatthanasiri, Ubol Paramee
The Thai Journal of Veterinary Medicine
The objectives of the present study were to evaluate the electroencephalography (EEG) patterns between healthy and epileptic dogs, to analyze the diagnostic value of scalp EEG for canine epilepsy under sedation with dexmedetomidine and to provide canine standard protocol. EEG of 29 epileptic dogs and 17 control dogs was recorded. Normal patterns, interictal epileptiform, and non-epileptiform abnormalities were interpreted using visual analysis by board-certified epileptologists. Results revealed that 34% of the dogs with epilepsy did not show discernible abnormalities on their EEG recordings. Approximately 38% of the epileptic dogs presented interictal epileptiform discharges, 28% had only non-epileptiform discharges, and 24% …
Prevalence And Distributions Of Feline Immunodeficiency Virus And Feline Leukemia Virus Infections In Bangkok And Its Vicinity, Thailand During 2013-2014,
2015
Chulalongkorn University
Prevalence And Distributions Of Feline Immunodeficiency Virus And Feline Leukemia Virus Infections In Bangkok And Its Vicinity, Thailand During 2013-2014, Teerawut Nedumpun, Patharapol Piamsomboon, Pattrarat Chanchaithong, Piyanan Taweethavonsawat, Sudchit Chungpivat, Sanipa Suradhat
The Thai Journal of Veterinary Medicine
Feline immunodeficiency virus (FIV) and Feline leukemia virus (FeLV) are known as pathogens associated with chronic diseases in cat. This present study determined the prevalence of FIV and FeLV infections in Bangkok and its vicinity, including Nonthaburi and Pathumthani, during April 2013-March 2014 in owned cats. A total of 777 serum or plasma samples were submitted to a private veterinary laboratory in Bangkok. The prevalence of FIV infection, FeLV infection and co-infection was 5.4% (42/777), 16.5% (128/777) and 3.5% (27/777), respectively. The infections were detected all year round, with higher prevalence of FeLV infection. In addition, geographical analyses revealed that …
In Vivo Activity Of Lactobacillus Plantarum On Reduction In Enterotoxigenic Escherichia Coli And Shiga Toxin-Producing E. Coli In Postweaning Pigs,
2015
Chulalongkorn University
In Vivo Activity Of Lactobacillus Plantarum On Reduction In Enterotoxigenic Escherichia Coli And Shiga Toxin-Producing E. Coli In Postweaning Pigs, Changhoon Park, Jiwoon Jeong, Kyuhyung Choi, Duck-Il Shin, A Rong Jeong, Sung-Hoon Kim, Chanhee Chae
The Thai Journal of Veterinary Medicine
The objective of this study was to determine the effect of Lactobacillus plantarum on enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) in order to reduce lesions and fecal shedding for the control of colibacillosis and edema disease in postweaning pigs. ETEC K88 colonized in greater abundance on the surface of the small intestinal tract of non-L. plantarum-fed pigs compared to L. plantarum-fed pigs. Reduced intestinal adherence and fecal shedding of ETEC K88 was observed in the L. plantarum-fed pigs, whereas brain lesions and fecal shedding of STEC F18 remained unchanged. The results of this study demonstrated that …
What Is Your Diagnosis?,
2015
Chulalongkorn University
What Is Your Diagnosis?, Nan Choisunirachon
The Thai Journal of Veterinary Medicine
No abstract provided.
Flash Visual Evoked Potentials In Normal Pomeranian Dogs And Those With Canine Cognitive Dysfunction,
2015
Chulalongkorn University
Flash Visual Evoked Potentials In Normal Pomeranian Dogs And Those With Canine Cognitive Dysfunction, Thanawut Hamnilrat, Chalermpol Lekcharoensuk, Piyathip Choochalermporn, Aree Thayananuphat
The Thai Journal of Veterinary Medicine
Visual evoked potential (VEP) is a useful technique for evaluation of optic nerve pathway and diagnosis of human Alzheimer’s disease. Canine cognitive dysfunction (CCD) is an age-related neurobehavioural syndrome which is commonly underdiagnosed in dogs. In the present study, VEPs were recorded from 28 Pomeranian dogs including 9 normal dogs aged between 1-8 years, 9 normal dogs aged over 8 years, and 10 dogs with signs of CCD. Brain atrophy in 8 CCD dogs was confirmed by CT scan. The other two dogs with signs of CCD had tumor which was confirmed by CT scan and cerebrospinal fluid. The VEP …
Response Of Gh, Acth, Tsh And Igf-I To Gonadotropin Releasing Hormone Stimulation In Ovariohysterectomized Rats,
2015
Chulalongkorn University
Response Of Gh, Acth, Tsh And Igf-I To Gonadotropin Releasing Hormone Stimulation In Ovariohysterectomized Rats, Jian-Liang Lin, Li-Shiue Shiu, Hsein-Chi Wang, Wei-Ming Lee
The Thai Journal of Veterinary Medicine
GnRH is not only a primary regulator of gonadal function but also a possible factor which affects other pituitary cells out of gonadotrophs. Recently, it has been reported that GnRH might induce the release of GH, but the effects on GH and other pituitary hormones of GnRH still remained controversial. In this study, OHE was performed in 30-week-old female Sprague Dawley rats as experimental groups. Intact female rats were taken as control group. GnRH stimulation at different weeks (20, 24, and 30) after OHE was examined in all rats. Evaluation of the circulating GH, IGF-I, ACTH, TSH concentrations at different …
