Detection Of The Contamination Sources Of Listeria Monocytogenes In Pickled White Cheese Production Process Line And Genotyping With The Pulsed-Field Gel Electrophoresis Method, Ni̇hat Telli̇, Ahmet Güner, Ferda Soyer Dönmez, Özgün Öykü Özdemi̇r
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the contamination sources, serotyping profiles, and antibiotic resistance patterns of Listeria monocytogenes isolated during the production of pickled white cheese. The genetic-relatedness of the isolates to EGD SLCC (5835) (1/2a, lineage II) and ATCC (13932) (4b, lineage I) reference strains was also determined with pulsed-field gel electrophoresis (PFGE) as a result of digestions with AscI and ApaI enzymes. Samples were collected from 16 different points in the production process of 4 different plants at 3 different times. Among the 192 samples examined, 17 (8.85%) were determined to be contaminated with Listeria spp. Three isolates …
Egg Production, Egg Quality, And Cecal Microbial Populations Of Layersfed Diets Supplemented With Fermented Phytogenic Feed Additive, Jae Hong Park, Tae Hwa Song, Inho Kim
Turkish Journal of Veterinary & Animal Sciences
This study was undertaken to examine the effect of fermented phytogenic feed additive (FPFA) containing Gynura procumbens and Rehmannia glutinosa on egg production, egg quality, and cecal microflora population in layers. In total, 288 Hy-Line brown layers were randomly divided into 4 dietary treatment groups with 6 replicate pens per treatment group for 5 weeks. The dietary treatments were the control group (CON; basal diet without FPFA), FPFA1 (CON + 0.5% FPFA), FPFA2 (CON + 1% FPFA), and FPFA3 (CON + 2% FPFA). Egg production and egg weight during the experiment (35-39 weeks) improved linearly with the supplementation of 1% …
Serological Survey For Antibodies Against Mycobacterium Avium Complexin Hunting Dogs In East-Central Portugal,
2016
TÜBİTAK
Serological Survey For Antibodies Against Mycobacterium Avium Complexin Hunting Dogs In East-Central Portugal, Ana Claudia Coelho, Manuela Matos, Luis Figueira, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Catarina Matos
Turkish Journal of Veterinary & Animal Sciences
The Mycobacterium avium complex (MAC) causes infections and diseases in humans, birds, livestock, and wildlife, among others. In dogs, MAC infection is not frequent; however, due to its potential zoonotic characteristics, mainly in immunocompromised patients, the study of MAC epidemiology is necessary. The aim of this work was to perform a study to detect the presence of antibodies against MAC in serum samples of 53 hunting dogs in the Idanha-a-Nova municipality of East-Central Portugal. A total of 23 dogs were seropositive by commercial ELISA test (43.4%, 95% CI: 30.1%-56.7%). Factors such as age, breed, and sex were not significant. The …
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes,
2016
TÜBİTAK
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Turkish Journal of Veterinary & Animal Sciences
Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …
Supplementation Effects Of Seleno-Compounds, Carnosic Acid, And Fish Oil Onconcentrations Of Fatty Acids, Tocopherols, Cholesterol, And Amino Acids In The Livers Of Lambs, Agnieszka Justyna Rozbicka-Wieczorek, Edyta Wiesyk, Katarzyna Agnieszka Krajewska-Bienias, Krzysztof Wereszka, Marian Czauderna
Turkish Journal of Veterinary & Animal Sciences
Lambs were fed a diet containing 3% rapeseed oil (RO) (the RO diet), a diet with 2% RO and 1% fish oil (FO) (the FO diet), or diets with combined additions of 2% RO, 1% FO, 0.1% carnosic acid (CA) (the CA diet), and 0.35 ppm Se as selenized yeast (SeY) (the CASeY diet) or selenate (SeVI) (the CASeVI diet). The CASeVI diet most effectively increased the body mass gain of lambs, while it most efficiently decreased the liver mass. The CASeVI diet most efficiently reduced the concentration of cholesterol and the lipid peroxidation in the liver. The CA diet …
Early Pregnancy Diagnosis Using A Commercial Elisa Test Based On Pregnancy-Associated Glycoproteins In Holstein-Friesian Heifers And Lactating Cows, Mehmet Sali̇h Kaya, Mehmet Köse, Faruk Bozkaya, Hasan Mutlu, Eyyüp Hakan Uçar, Mehmet Osman Atli
Turkish Journal of Veterinary & Animal Sciences
In this study, we aimed to investigate the efficacy of a commercial ELISA test kit for detecting pregnancy-associated glycoproteins (PAGs) in peripheral blood for early pregnancy diagnosis and compare plasma PAG levels during early pregnancy in both Holstein-Friesian heifers and lactating cows. A total of 231 plasma samples were collected from heifers and lactating cows on days 25, 28, and 32 after insemination. Pregnancies were confirmed 30 days after the collection of plasma samples. Plasma PAG levels were measured using a commercial ELISA test kit for diagnosing bovine pregnancy. The effects of examination date and animal status (heifers vs. lactating …
The Effect Of Feeding Rhizopus Oligosporus On Growth Performance, Nutrient Digestibility, Blood Profile, Fecal Microbiota, And Fecal Score In Weanling Pigs, Jae Hong Park, Hyeok Min Yun, In Ho Kim
Turkish Journal of Veterinary & Animal Sciences
Piglets were allocated to 1 of 3 treatments by body weight and sex, and fed different levels of 0%, 0.15%, and 0.30% of R. oligosporus 1.0 x 106 cfu/g, in a basal diet based on corn-soybean meal for 35 days. Piglets fed diets supplemented with R. oligosporus had higher average daily gain during days 15-35 and 1-35 (quadratic, P < 0.05). Feed conversion ratio during 1-35 days showed a significant improvement, as dietary R. oligosporus supplementation increased from 0.15% to 0.30% (linear, P < 0.05). Apparent total tract digestibility (ATTD) of crude protein (CP) was increased at 2 weeks, as dietary R. oligosporus supplementation increased significantly (linear and quadratic, P < 0.05), and ATTD of dry matter, CP, and energy were linearly increased at 5 weeks (P < 0.05). The serum IgG concentration was significantly increased in the R. oligosporus-fed groups (quadratic, P < 0.05). Fecal Lactobacillus counts were significantly increased quadratically and coliform bacteria counts were decreased linearly, as dietary R. oligosporus supplementation increased (P < 0.05). In conclusion, diets containing R. oligosporus may improve the growth performance, nutrient digestibility, IgG concentration, and intestinal environment in weanling pigs.
Occurrence And Antimicrobial Resistance Of Salmonella Enterica Subsp. Enterica Serovars Typhimurium, Enteritidis, And Typhi Isolated From Chicken Eggs And Poultry Products, Serhat Al, Harun Hizlisoy, Nurhan Ertaş Onmaz, Yeli̇z Yildirim, Zafer Gönülalan
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to detect the occurrence and antimicrobial resistance of Salmonella enterica subsp. enterica serovars Typhimurium, Enteritidis, and Typhi in 252 samples including 100 chicken eggs, 60 processed poultry products (20 nuggets, 20 salami samples, 20 sausages), and 92 poultry giblets (50 livers and 42 gizzards). Salmonella spp. and serovars were identified by PCR. The antimicrobial susceptibility testing was performed by disk diffusion method. Forty-seven (31%) of 152 poultry products and 5 (5%) of 100 egg samples were positive for Salmonella spp. Salmonella spp. was positive in 3.3%, 5%, 18%, and 27% of chicken nugget, sausage, gizzard, …
Molecular Identification Of Bovine Papillomaviruses In Dairy And Beef Cattle: First Description Of Xi- And Epsilonpapillomavirus In Turkey, Veysel Soydal Ataseven, Özgür Kanat, Yaşar Ergün
Turkish Journal of Veterinary & Animal Sciences
In the current study, 23 papilloma/tumor-like samples obtained from cattle having clinical lesions and 9 blood samples collected from healthy-appearing cattle in Turkey were examined for bovine papillomavirus (BPV) DNA using the degenerate primers FAP59/64, and the different types of BPV were distinguished by type-specific primer sets. Furthermore, histopathological studies of papillomavirus were performed. A total of 7 BPV types (BPVs 1, 2, 3, 4, 6, 8, 9), including genera Deltapapillomavirus, Xipapillomavirus, and Epsilonpapillomavirus, were identified. In all samples, BPV-1 was the most common genotype (90.6%). Overall, coinfections were determined in 26 of the examined samples (81.3%), and coinfection with …
Selecting Eimeria Tenella-Resistance Markers By Comparing Resistant And Susceptible Groups Of Jinghai Yellow Chickens,
2016
TÜBİTAK
Selecting Eimeria Tenella-Resistance Markers By Comparing Resistant And Susceptible Groups Of Jinghai Yellow Chickens, Jingjing Zhang, Yuxin Lin, Guojun Dai, Tingting An, Mingming Sun, Genxi Zhang, Kaizhou Xie, Jingyu Wang
Turkish Journal of Veterinary & Animal Sciences
Improving the genetic resistance of chickens to parasitic diseases is an alternative way to protect the poultry industry. To establish valid measurable in vivo parameters indicating coccidiosis resistance, 232 Jinghai Yellow chickens were randomly selected and orally infected with Eimeria tenella (1.5 x 104 sporulated oocysts per chicken). The resistant and susceptible chickens were classified based on their cecal lesion scores 8 days after infection. Ten plasma components, including antioxidant enzymes, interleukins (ILs), nitric oxide (NO), interferon-γ (IFN-γ), and β-carotene, were compared among the resistant, susceptible, and control chickens (uninfected with E. tenella). The NO, superoxide dismutase (SOD), IL17, and …
Bacteria Associated With Clinical Postpartum Dysgalactia Syndrome In Farmed Sows In The Republic Of Macedonia,
2016
TÜBİTAK
Bacteria Associated With Clinical Postpartum Dysgalactia Syndrome In Farmed Sows In The Republic Of Macedonia, Branko Angjelovski, Aleksandar Cvetkovikj, Slavcho Mrenoshki, Miroslav Radeski, Iskra Cvetkovikj, Marija Ratkova, Toni Dovenski
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to gather information about the prevalence of bacterial species in farmed sows, with special focus on the clinical manifestation of postpartum dysgalactia syndrome (PDS). One hundred and sixteen sows from 5 pig farms in the Republic of Macedonia were clinically examined for PDS 12?24 h after farrowing. Milk samples and vaginal swabs for bacteriological testing were taken from PDS-affected (PDSA, n = 30) and PDS-unaffected (PDSU, n = 86) sows. Escherichia coli, staphylococci, and streptococci were the predominant bacteria isolates. Escherichia coli was the most frequently found isolate, with a prevalence of 73.3% in …
Protective Effects Of S-Adenosylmethionine (Same) And Silybin On Hepatorenal And Hemostatic Functions In Dogs With Endotoxemia,
2016
TÜBİTAK
Protective Effects Of S-Adenosylmethionine (Same) And Silybin On Hepatorenal And Hemostatic Functions In Dogs With Endotoxemia, Meri̇ç Kocatürk, Oya Eralp İnan, Pinar Levent, Zeki̇ Yilmaz
Turkish Journal of Veterinary & Animal Sciences
We aimed to investigate the protective effects of S-adenosylmethionine (SAMe) and silybin treatment on hepatorenal and hemostatic functions in dogs with endotoxemia. Healthy dogs (n = 20) were divided equally into 4 groups as control, SAMe + silybin (SS), lipopolysaccharide (LPS), and LPS + SS. Controls received 0.9% NaCl intravenously. Animals were treated daily with 20 mg of SAMe and 1 mg of silybin per kilogram of body weight in SS and LPS + SS. LPS was injected (2 μg/kg, once intravenously) to dogs in LPS and LPS + SS. Hemostatic functions were assessed by thromboelastography (TEG). LPS-induced clinical and …
Influence Of Season On Seminal Antioxidant Enzymes In Karan Friesbulls Under Tropical Climatic Conditions,
2016
TÜBİTAK
Influence Of Season On Seminal Antioxidant Enzymes In Karan Friesbulls Under Tropical Climatic Conditions, Simson Soren, Sohan Vir Singh, Pawan Singh
Turkish Journal of Veterinary & Animal Sciences
Availability of good quality semen is very crucial for sustainable development of the dairy sector throughout the year. Seminal antioxidant status plays an important role for sperm viability, acrosomal integrity, and motility and emerges as a prognostic tool for assisted reproductive technology. The present study was designed to evaluate the seminal antioxidant enzyme concentrations in Karan Fries bulls during different seasons. Five adult healthy Karan Fries (Bos indicus x Bos taurus) bulls were selected from the the Animal Breeding Research Centre of the Indian Council of Agricultural Research-National Dairy Research Institute, Karnal. Weekly ejaculates were collected using an artificial vagina …
Effect Of Capacitating Agents On Sperm Pretreatment During In Vitrofertilization For Blastocyst Production In Caprines,
2016
TÜBİTAK
Effect Of Capacitating Agents On Sperm Pretreatment During In Vitrofertilization For Blastocyst Production In Caprines, Puja Goel, Anil Kumar Goel, Ashok Kumar Bhatiya, Suresh Dinkar Kharche
Turkish Journal of Veterinary & Animal Sciences
The present study aimed at comparing the relative efficacy of different capacitating agents used for sperm pretreatment during in vitro fertilization (IVF) and subsequent embryo development of caprine oocytes. In experiment 1, the effect of different concentrations of heparin (0, 20, and 50 μg/mL) on sperm pretreatment for different periods of time (30 and 60 min) was studied. In experiment 2, the dose-dependent effect of calcium ionophore (0, 0.1, 0.2, and 0.5 μM) on sperm pretreatment was assessed. Experiment 3 validated and compared the effects of both capacitating agents on sperm pretreatment for capacitation based on the results of the …
Seroprevalence Of Caseous Lymphadenitis In Kangal Akkaraman Sheep,
2016
TÜBİTAK
Seroprevalence Of Caseous Lymphadenitis In Kangal Akkaraman Sheep, Öznur Aslan, Kadi̇r Semi̇h Gümüşsoy, İlknur Karaca Bekdi̇k, Aytaç Akçay, Ömer Orkun Demi̇ral
Turkish Journal of Veterinary & Animal Sciences
In this study, the seroprevalence of Corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis (CLA) infection, in Kangal Akkaraman sheep flocks was examined. For this purpose, blood samples were collected from 25 different sheep flocks (n = 379). According to the ELISA results, the numbers of CLA-positive and CLA-negative samples were 138 (36.4%) and 241 (63.6%), respectively. CLA positivity was observed in 35.7% of the females (n = 241), 37.7% of the males (n = 138), 40.2% of the sheep with clinical symptoms (n = 184), and 32.8% of the sheep with no clinical symptoms (n = 195) (P > 0.05). …
The Ability Of Bull And Stallion Thawed Spermatozoa Refrozen Without Cryoprotectants To Activate Intra- And Interspecies Oocytes,
2016
Louisiana State University and Agricultural and Mechanical College
The Ability Of Bull And Stallion Thawed Spermatozoa Refrozen Without Cryoprotectants To Activate Intra- And Interspecies Oocytes, Jose Len Yin
LSU Doctoral Dissertations
Semen cryopreservation has allowed the establishment of genome banks and the large scale propagation of species. The development of simple techniques to cryopreserve semen or alternatives to efficiently use cryopreserved semen from males of valuable genetics that have become infertile will permit continuous propagation of the genetics from these males and may serve as a model for preservation and propagation of endangered species. Sperm cryopreservation without cryoprotectants is a simple process, and offspring have been produced following intracytoplasmic sperm injection (ICSI); however the ability of frozen-thawed sperm refrozen without the addition of cryoprotectants to activate oocytes following ICSI was unknown. …
Amblyomma Maculatum Immunomodulation In Mammalian Models Of Rickettsia Parkeri Rickettsiosis,
2016
Louisiana State University and Agricultural and Mechanical College
Amblyomma Maculatum Immunomodulation In Mammalian Models Of Rickettsia Parkeri Rickettsiosis, Kaikhushroo Hormazd Banajee
LSU Doctoral Dissertations
Rickettsia parkeri is an emerging human pathogen and spotted fever group Rickettsia that is transmitted via Amblyomma maculatum (the Gulf Coast tick) in the United States. Since these ticks must feed for several days in order to molt to the next life cycle, they must be able to counteract the host immune response. Despite this fact, there have been few studies that evaluate the immunomodulatory effect of this vector and the resultant influence on rickettsial disease. The hypothesis of this research is that, if A. maculatum feeding modifies the host immune response, this immunomodulation will enhance disease caused by R. …
Identification And Characterization Of A Relish-Type Nf-Kb, Dvrelish In Dermacentor Variabilis, The American Dog Tick,
2016
Louisiana State University and Agricultural and Mechanical College
Identification And Characterization Of A Relish-Type Nf-Kb, Dvrelish In Dermacentor Variabilis, The American Dog Tick, Victoria Irene Verhoeve
LSU Doctoral Dissertations
Ticks are important worldwide as vectors of bacteria, viruses, and parasites. Pathogenic and non-pathogenic Spotted fever group (SFG) Rickettsia are maintained and transmitted by ticks with specific hard tick-Rickettsia pairings evident in nature. The pathogenic SFG Rickettsia rickettsii is transmitted by the hard tick Dermacentor variabilis. In response to infection, D. variabilis is known differentially respond to SFG Rickettsia infection. The mechanisms of differential immune induction are currently unknown, and are likely involved in the establishment of specific tick-SFG Rickettsia pairings. It was hypothesized that the level of response by D. variabilis to SFG Rickettsia occurs in a species-specific manner, …
The Role Of Nadph Oxidase 2 (Nox2) In High-Fat Diet-Induced Adiposopathy And Brain Dysfunction In A Mouse Model,
2016
Louisiana State University and Agricultural and Mechanical College
The Role Of Nadph Oxidase 2 (Nox2) In High-Fat Diet-Induced Adiposopathy And Brain Dysfunction In A Mouse Model, Jennifer Kathleen Pepping
LSU Master's Theses
Obesity can have numerous detrimental consequences, namely metabolic syndrome, type 2 diabetes, cardiovascular disease, cancer, and Alzheimer’s disease. The pathogenesis and physiologic consequences of obesity are unknown, but they are often associated with increased inflammation and oxidative stress in both the body and in the brain. One factor that has been implicated in causing inflammation associated with a high fat diet is the enzyme NADPH oxidase, or NOX, specifically the subunit NOX2. Two studies were performed in order to assess the effects of a high fat diet in combination with a universal NOX2 deficiency and a NOX2 deficiency targeted to …
Chikungunya Virus Infection-Associated
Bone And Joint Disease,
2016
Louisiana State University and Agricultural and Mechanical College
Chikungunya Virus Infection-Associated Bone And Joint Disease, Brad A. Goupil
LSU Doctoral Dissertations
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that circulates predominantly in tropical and subtropical regions. Infection results in severe debilitating polyarthralgia during the acute phase of disease, and reports suggest that chronic arthralgia lasting months to years after the initial infection can occur. More severe and prolonged disease has been associated with pre-existing joint disease, though this has not been experimentally examined. In the research presented herein, two established mouse models (adult IRF 3/7 -/- -/- and wild-type C57BL/6J mice) were utilized to characterize CHIKV-associated bone and joint disease and evaluate its impact on the progression of pre-existing osteoarthritis (OA) …
