Veterinary Medicine Commons^™

Visualizing Herpesvirus Procapsids In Living Cells, Oana Maier, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

A complete understanding of herpesvirus morphogenesis requires studies of capsid assembly dynamics in living cells. Although fluorescent tags fused to the VP26 and pUL25 capsid proteins are available, neither of these components is present on the initial capsid assembly, the procapsid. To make procapsids accessible to live-cell imaging, we made a series of recombinant pseudorabies viruses that encoded green fluorescent protein (GFP) fused in frame to the internal capsid scaffold and maturation protease. One recombinant, a GFP-VP24 fusion, maintained wild-type propagation kinetics in vitro and approximated wild-type virulence in vivo. The fusion also proved to be well tolerated in …

Baylisascaris Larva Migrans, Kevin R. Kazacos

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Zoonotic diseases, such as baylisascariasis, are receiving increasing attention as components of disease emergence and resurgence (Kazacos, 2001). Baylisascariasis is caused by the roundworm Baylisascaris procyonis and is one of the more recent zoonotic disease developments. This disease remains one of the least known and poorly understood zoonotic diseases, yet over the past several decades it has become widespread. It originated in wildlife species and is now well established as a human malady. Baylisascariasis is transmitted to humans via consumption of contaminated feces, and the role of wildlife (primarily raccoons) in this transmission process is becoming more clearly known and …

In Vivo-Expressed Proteins Of Virulent Leptospira Interrogans Serovar Autumnalis N2 Elicit Strong Igm Responses Of Value In Conclusive Diagnosis, Veerapandian Raja, Santhanam Shanmughapriya, Murugesan Kanagavel, Sergey Artiushin, Sridhar Velineni, John F. Timoney, Kalimuthusamy Natarajaseenivasan

Maxwell H. Gluck Equine Research Center Faculty Publications

Leptospirosis is a serious zoonosis that is underdiagnosed because of limited access to laboratory facilities in Southeast Asia, Central and South America, and Oceania. Timely diagnosis of locally distributed serovars of high virulence is crucial for successful care and outbreak management. Using pooled patient sera, an expression gene library of a virulent Leptospira interrogans serovar Autumnalis strain N2 isolated in South India was screened. The identified genes were characterized, and the purified recombinant proteins were used as antigens in IgM enzyme-linked immunosorbent assay (ELISA) either singly or in combination. Sera (n = 118) from cases of acute leptospirosis along …

Growing South Dakota (Winter 2016), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2015 South Dakota Agricultural Experiment Station Annual Report.

[Page] 2 Director's Message
[Page] 3 Concentrating On Cattle: Researcher Studying Mechanics To Improve Reproduction Efficiency In Beef Cattle
[Page] 5 At The Forefront: SDSU Diagnostic Lab Scientists Sequence Avian Influenza Genome
[Page] 6 Seeking Superior Wheat: Breeder Using Extensive Genome Testing To Improve Winter Wheat Cultivars
[Page] 8 Profiles In Leadership: Plant And Fungi Interactions May Offer New Plant Production Possibilities
[Page] 9 Targeting Emerging Diseases: Plant Pathologists Working To Better Manage Soybean & Sunflower Diseases
[Page] 10 Compelling New Crop: Carinata Offers Potential For Crop Diversity, …

Circulation Of Schmallenberg Virus In Turkey, 2013, Şükrü Tonbak, Ahmet Kürşat Azkur, Züleyha Pesti̇l, Emel Biyikli, Hasan Abayli, Ersoy Baydar, Wim H.M. Van Der Poel, Hakan Bulut

Turkish Journal of Veterinary & Animal Sciences

Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3%) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, …

Estimation Of Stray Dog And Cat Populations In Metropolitan Ankara, Turkey, Doğukan Özen, Dankmar Böhning, İsmayi̇l Safa Gürcan

Turkish Journal of Veterinary & Animal Sciences

Stray dogs and cats contribute to serious health problems in human societies around the world. Before any necessary interventions to control the stray dogs and cats, an accurate estimate of their populations should be attained. Yet, there is a very limited number of methods for an estimation. Some of them depend on the identification of marked or counted animals. However, problems arise when it is not possible to identify a previously captured animal. In this paper, we used a different approach to estimate the lower bound for the total number of dogs and cats in consecutively visited settlements that might …

Comparative Evaluation Of Factors Affecting Hemagglutinatingactivity Of Avian Influenza (H9) Virus, Asfa Rasool, Sameera Akhtar, Khushi Muhammad, Masood Rabbani, Aftab Ahmed Anjum, Javed Muhammad, Arfan Ahmad, Ali Ahmad Sheikh, Fakhra Liaqat, Fariha Akhtar, Rabia Tahir

Turkish Journal of Veterinary & Animal Sciences

Influenza virus H9 was standardized for hemagglutination assay using different factors such as red blood cell (RBC) types, concentrations, diluent types, and storage times. Avian influenza virus H9 was grown in embryonated chicken eggs and confirmed by spot agglutination. A significant (P < 0.05) difference with the highest mean titer (9.00 ± 0.00) was observed using RBCs from different species. Nonsignificant differences (P > 0.05) were found between human blood type O, chicken, and dog RBCs, as well as among rabbit, pigeon, sheep, and parrot. The highest titer (9.00 ± 0.00) with a nonsignificant difference was found using virus stored at 4 °C and -20 °C while 37 °C showed the lowest significant mean hemagglutinin (HA) titer (11.08 ± 188.21). Nonsignificant differences …

Effects Of Mycobacterium Phlei On Intestinal Microecology Andserum Immunological Parameters Of Weaned Piglets, Jin-Feng Zhong, Xiao-Qing Zhang, Wei-Gao Wu, Wei Tu, Zhen-Xiang Liu, Re-Jun Fang

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of Mycobacterium phlei in diets (Experiment 1) and investigate the mode of action on the intestinal microecology of weaned piglets (Experiments 1 and 2 ). In Experiment 1, a total of 96 commercial cross-bred pigs [(Duroc × Yorkshire) × Landrace], weaned at 21 days, were assigned to two treatments in a completely randomized design with a single factorial arrangement of 40 days of supplementation (nonsupplemented vs. supplemented with 0.4% heat-killed M. Phlei). The results showed that M. Phlei has the capability to improve the intestinal microecology of weaned piglets. The …

Ultrasonographic Features Of Medial Iliac And Jejunal Lymph Nodesin Apparently Healthy Dogs, Shriram Ganesan, Jitender Mohindroo, Pallavi Verma, Narinder Singh Saini

Turkish Journal of Veterinary & Animal Sciences

Ultrasonographic examination of medial iliac and jejunal lymph nodes were performed in 36 dogs presented to the Department of Surgery and Radiology for spaying and castration. Ultrasonographic features such as length, height in longitudinal plane, shape, ease of scanning, echotexture, and echogenicity were recorded. The medial iliac and jejunal lymph nodes were scanned consistently in all 36 dogs. However, the presence of gas in the intestines affected the ease of scanning of the lymph nodes. The majority of these lymph nodes were easy to scan, and had homogeneous and hypoechoic echogenicity. The dogs were grouped based on body weight and …

Seroepidemiological Survey Of Bovine Tick-Borne Infections In Theblack Sea Region Of Turkey, Mustafa Açici, Cenk Soner Bölükbaş, Gökmen Zafer Pekmezci̇, Ali̇ Tümay Gürler, Şi̇nasi̇ Umur, Kadri̇ Zafer Karaer, Ayşe Çakmak, Ayşe Serpi̇l Nalbantoğlu, Cevat Ni̇sbet

Turkish Journal of Veterinary & Animal Sciences

Babesiosis, theileriosis, and anaplasmosis are the tick-borne diseases of cattle in most of the tropical areas and Turkey. A total of 270 cattle were randomly selected from 27 villages and 77 farms in the Black Sea Region of Turkey for the determination of infections by Babesia bigemina and Anaplasma marginale by using the indirect fluorescent antibody (IFA) test and the competitive enzyme-linked immunosorbent assay (cELISA). Of those, 242 and 256 sera were also analyzed for antibodies against Theileria annulata and Babesia bovis infections using the IFA test, respectively, during the period of June 2006 to June 2007. Of the 256 …

Lipid And Protein Metabolism Along With Oxidative Status Of Follicular Fluidthroughout The Estrous Cycle In Anatolian Water Buffalo, Oktay Yilmaz, Jerzy Jan Jaroszewski, Azi̇z Bülbül, Mehmet Uçar

Turkish Journal of Veterinary & Animal Sciences

This study was performed to determine the concentrations of creatinine (CRE), high-density lipoprotein (HDL), low-density lipoprotein (LDL), blood urea nitrogen (BUN), nitric oxide (NO), and malondialdehyde (MDA) in follicular fluid (FF) in relation to follicular size and/or stage of the estrous cycle [early I luteal (n = 14), early II luteal (n = 16), late luteal (n = 16), and follicular (n = 16) phases] in Anatolian water buffaloes. The concentrations of CRE (P < 0.001), HDL (P < 0.001), LDL (P < 0.05) BUN (P < 0.001), and NO (P < 0.01) in FF of large follicles (LFs, ?10 to

Atopic Dog Skin Shows Decrease Of Claudin-1 But Increase Of Atopic Signature Cytokines, Sirin Theerawatanasirikul, Pakawadee Pongket, Lawan Larsuprom, Amornrate Sastravaha

Turkish Journal of Veterinary & Animal Sciences

Canine atopic dermatitis is a common allergic skin disease in dogs caused by defects in immunological and epidermal barriers. Claudin-1, a protein present in tight junctions, is associated with many skin conditions. Our aim was to investigate expression levels of claudin-1 and atopic signature cytokines in atopic and normal dog skins. The affected skin showed significantly decreased intensity and epidermal distribution of claudin-1 compared to the normal control skin (P < 0.05). The CD3+/CD4+ and CD3+/CD8+ T cell subsets, eosinophils, and neutrophils infiltrated the affected epidermis and dermis. The CD3+/CD4+ T cell subsets in lesional skin were significantly higher than CD3+/CD8+ T cell subsets. CLDN-1 mRNA expression was markedly downregulated in the lesional skin, while IL-17A was upregulated in both lesional and nonlesional skin. IL-4 and IL-31 mRNA was upregulated in the lesional skin, and TNF-α mRNA was significantly upregulated in the nonlesional skin (P < 0.01 and P < 0.05, respectively). Correlation-based hierarchical clustering showed that CLDN-1 expression was closely clustered with IL-31 but loosely clustered with L-4, IL-10, IL-13, and IL-17A. We suggest that the alteration of claudin-1 in atopic dogs may disrupt the skin barrier and allow an influx of allergens, inciting inflammatory cytokine responses.

Comparative Study Between Formalin-Killed Vaccine And Developed Gammairradiation Vaccine Against Mannheimia Haemolytica In Rabbits, Sahar Ahmed, Basem Ahmed, Ghada Mahmoud, Waleed Nemr, Emam Abdel Rahim

Turkish Journal of Veterinary & Animal Sciences

Mannheimia haemolytica is responsible for considerable economic losses to sheep, goats, and cattle and other livestock industries in Egypt. This study aimed to evaluate the effectiveness of a newly developed gamma irradiation vaccine against Mannheimia haemolytica in comparison to a formalin-killed vaccine. Three groups of rabbits were used in this study. Group 1 animals were inoculated with 4 × 109 bacterial cells per dose of the formalin-killed vaccine. Group 2 was inoculated with 2 × 109 bacterial cells per dose of gamma-irradiated vaccine. Group 3 (control group) was injected with 2 mL of sterile PBS. The vaccines were injected subcutaneously …

High Level Fluoroquinolone Resistance In Escherichia Coli Isolatedfrom Animals In Turkey Is Due To Multiple Mechanisms, Pinar Şahi̇ntürk, Erdem Arslan, Esra Büyükcangaz, Songül Sonal, Ayşi̇n Şen, Fi̇gen Ersoy, Mark A. Webber, Laura Jv. Piddock, Murat Cengi̇z

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the molecular mechanisms of fluoroquinolone resistance in E. Coli isolated from cattle, goats, sheep, cats, and dogs in Turkey. Twenty nonreplicate E. Coli isolates (chosen on the basis of RAPD pattern) from food-producing animals were selected for the study. To identify phenotypic differences between isolates, the sum of the MIC values of 14 antimicrobials was calculated; values ranged from 565 to 2520 μg/mL, indicating the diversity in antimicrobial resistance present in the panel of isolates. PCR and qRT-PCR were used to characterize the presence and expression levels of known molecular mechanisms of …

Mycoflora And Chemotype Characterization Of Fusarium Graminearum Isolatesobtained From Corn And Barley Used As Feedstuffs In The Northeast Of Iran, Abdollah Salehi, Roya Rezaeian Doloei

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate the mycoflora and chemotyping of Fusarium graminearum isolates in corn and barley used in industrial dairy farms in Khorasan-Razavi Province, northeastern Iran. For this purpose, a total of 128 corn and barley samples were collected from 16 different large industrial dairy farms. Mean moisture of the samples collected in different seasons of the year was significantly different (P < 0.05). The most prevalent fungi isolated from corn samples were Penicillium spp. (90.62%) and Aspergillus spp. (82.81%), and that of barley samples was Penicillium spp. (89.06%). Frequency of Fusarium spp. İn corn and barley samples was 85.93% and 26.56%, respectively. All isolates (100%) were confirmed as Fusarium using an ITS primer. Eight (11.11%) out of 72 Fusarium spp. İsolates were confirmed as F. Graminearum using species-specific primers. Five out of 8 F. Graminearum isolates were positive in terms of the presence of the trichothecene-producing gene using a Tri5-specific primer. Three and 2 out of 5 toxin-producing F. Graminearum isolates were positive using Tri13DON- and Tri13NIV-specific primers, respectively. In conclusion, the presence of toxigenic Fusarium in corn and barley samples used in feedstuffs in Iran is a potential risk to animal and human health.

Rickettsia Felis, Transmission Mechanisms Of An Emerging Flea-Borne Rickettsiosis, Lisa Diane Brown

LSU Doctoral Dissertations

Rickettsia felis is an emerging insect-borne rickettsial pathogen and the causative agent of flea-borne spotted fever. First described as a human pathogen from the United States in 1991, R. felis is now identified throughout the world and considered a common cause of fever in Africa. The cosmopolitan distribution of this pathogen is credited to the equally widespread occurrence of cat fleas (Ctenocephalides felis), the primary vector and reservoir of R. felis. Additionally, R. felis has been identified in other hematophagous arthropods (including numerous species of fleas, ticks, mosquitoes, and mites). Most transmission cycles of pathogenic Rickettsia include transovarial and transstadial …

Characterization Of Rickettsial Infection Dynamics Within Dermacentor Variabilis And Amblyomma Maculatum, Emma Kate Harris

LSU Doctoral Dissertations

Spotted fever group (SFG) Rickettsia are primarily associated with their reservoir host and vector, the tick. Rickettsial colonization and maintenance within the arthropod is a key component of vector competence and pathogen transmission to the mammalian host. Contemporary detection of novel tick hosts for rickettsial species, combined with an unprecedented rise in human cases of SFG rickettsiosis, necessitates a deeper understanding of tick/Rickettsia interactions. The hypothesis for this work is that if primary tick/Rickettsia pairings do not exist then rickettsial determinants account for primary vector/pathogen relationships. To this end, Dermacentor variabilis and Amblyomma maculatum ticks were exposed to R. rickettsii, …

The Effects Of Varying Levels Of Dietary Starch On Reproductivetraits In Lactating Dairy Cows, Abdulkadi̇r Keski̇n, Hidir Gençoğlu, Gülnaz Meci̇toğlu, İsmai̇l Çeti̇n, Çağdaş Kara, Duygu Udum Küçükşen, Ebru Bi̇len, Bariş Güner, Abdülkadi̇r Orman, Ahmet Gümen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of three different dietary starch levels on postpartum milk yield, blood metabolites, and reproductive traits in cows. A total of 23 primiparous Holstein-Friesian cows were fed three different diets including different starch levels on a dry matter basis from parturition until day 80. Cows were randomly assigned into the three groups according to the starch levels: low (LS, 16%, n = 7), medium (MS, 20%, n = 8), and high (HS, 24%, n = 8). Milk yields were recorded daily, and body condition score (BCS) was determined every week. Transrectal …

Diagnostic And Therapeutic Approach To Cardiac Myxosarcoma In A Dog, Meri̇ç Kocatürk, Hakan Salci, Murat Bi̇çer, Ahmet Akkoç, Sai̇m Sağ, Zeki̇ Yilmaz

Turkish Journal of Veterinary & Animal Sciences

A 7-year-old Chow Pei was referred to an animal hospital for the evaluation of a 1-month history of abdominal distension and exercise intolerance. A systolic murmur was auscultated over the tricuspid valve (TV). Thoracic radiographs showed cardiomegaly, right atrial enlargement, and pulmonary edema. Electrocardiography revealed small QRS complexes and a right-bundle brunch block. Echocardiography also showed right atrial enlargement and tricuspid insufficiency due to the presence of a mass on the posterior leaflet of the TV. The mass was excised by intracardiac surgery and histological evaluation revealed myxosarcoma. This is a case report of a diagnostic and therapeutic approach to …

Evaluation Of Isoflurane Anesthesia After Xylazine/Ketamineadministration In Dromedary Camels, Fahd Abdullah Alsobayil, Ahmed Fathy Ahmed, Omar Salaheddin Eltookhy

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate isoflurane after premedication with xylazine and induction with ketamine in camels. Six healthy adult female dromedary camels were premedicated with xylazine (0.2 mg/kg, IV). Twenty minutes later, anesthesia was induced with ketamine (2 mg/kg, IV) and was maintained with isoflurane in 100% oxygen. Onset and duration of anesthesia were recorded. Rectal temperature, respiratory rate, heart rate, oxygen hemoglobin saturation, and blood pressure were measured before and 20 min after xylazine administration and every 10 min thereafter until recovery. Lead II electrocardiogram was used to monitor camels for the presence of arrhythmias. Venous …