Genetic Detection And Identification Of Chlamydophila Psittaci In Captive Psittacine Birds In Thailand,
2016
Chulalongkorn University
Genetic Detection And Identification Of Chlamydophila Psittaci In Captive Psittacine Birds In Thailand, Parut Suksai, Benchapol Lorsunyaluck, Patcharida Dittawong, Pornchai Sanyathitiseree, Preeda Lertwatcharasarakul
The Thai Journal of Veterinary Medicine
Chlamydophilosis is an important, worldwide zoonosis caused by Chlamydophila psittaci. The aim of this study was to detect C. psittaci on choanal and cloacal swabs which were collected from 178 captive psittacine birds (23 different species) from eight provinces in Thailand. ompA gene detection using nested PCR showed that 7.87% (n=14) were positive for C. psittaci. The C. psittaci detection from two site collections (choanal and cloacal swabs) using nested PCR was more sensitive than from one site collection. Most positive samples (n=11) were from asymptomatic adult birds and three other positive birds showing clinical signs were juvenile birds. As …
Influence Of Oil Or Fat Supplementation On Rumen Fermentation Characteristics And Ruminal Fluid Fatty Acid Profile In Brahman Crossbred Fattening Steers,
2016
Chulalongkorn University
Influence Of Oil Or Fat Supplementation On Rumen Fermentation Characteristics And Ruminal Fluid Fatty Acid Profile In Brahman Crossbred Fattening Steers, Pitunart Noosen, Pipat Lounglawan, Wisitiporn Suksombat
The Thai Journal of Veterinary Medicine
The aim of this experiment was to study the influence of oil or fat supplementation on rumen fermentation and ruminal fluid fatty acid profiles in Brahman crossbred steers. Twelve steers (87.5% Brahman crossbred) with average live weight (LW) of 337+54 kg and approximate age of 2 years old were stratified by their LW into 4 groups and each group was randomly assigned to four dietary treatments. All steers were fed 14% CP concentrate. The treatments included: 1) 7 kg/d concentrate, 2) 4 kg/d concentrate supplemented with 200 g/d palm oil (PO), 3) 4 kg/d concentrate supplemented with 100 g/d PO …
Effect Of Antibiotic Growth Promoters On Anti-Oxidative And Anti-Inflammatory Activities In Broiler Chickens,
2016
Chulalongkorn University
Effect Of Antibiotic Growth Promoters On Anti-Oxidative And Anti-Inflammatory Activities In Broiler Chickens, Krittika Kabploy, Nuntavan Bunyapraphatsara, Noppawan Phumala Morales, Nuanchan Paraksa
The Thai Journal of Veterinary Medicine
The current study was conducted to evaluate the effects of sub-therapeutic antibiotics (avilamycin and flavophospholipol) in animal feed on anti-oxidative and anti-inflammatory properties in broilers to understand the mechanisms of AGPs. Four hundred and fifty six 1-day-old broilers (ROSS 308) were divided into 3 groups with 4 replications. Three experimental diets composed of a corn-soybean meal based diet supplemented with 0, 10 mg/kg avilamycin and 5 mg/kg flavophospholipol were randomly provided to the broilers for 42 days, respectively. Results showed that the AGPs significantly decreased TBA-MDA adduct in serum as well as villi tissue of the broilers, especially flavophospholipol. However, …
Distribution Of Tem-1 Gene In Salmonella Enterica Isolated From Poultry Carcasses In Iran,
2016
Chulalongkorn University
Distribution Of Tem-1 Gene In Salmonella Enterica Isolated From Poultry Carcasses In Iran, Abbas Doosti, Ali Zohoor, Mohammad Chehelgerdi, Abbas Mokhtari-Farsani
The Thai Journal of Veterinary Medicine
Salmonella is one of the most important bacteria isolated from poultry and a major source of bacterial infection to human. The aim of present research was to study TEM-1 beta-lactamase gene in serovars of S. enterica subsp. enterica isolated from poultry carcasses samples in Iran. A total of 600 samples of poultry carcasses were collected and Salmonella was isolated from the samples using bacterial culture methods and genomic DNA was extracted using DNA extraction kit. For the final approval of Salmonella, identification of S. enterica subsp. enterica, S. enterica serovars and TEM-1 gene sequence-specific targets (16S rRNA, fliC, rfbJ, fljB …
Comparison Of Two Methods For The Recovery Of Campylobacter From Chicken Carcasses,
2016
Chulalongkorn University
Comparison Of Two Methods For The Recovery Of Campylobacter From Chicken Carcasses, Suthida Muangnoicharoen, Sakaoporn Prachantasena, Luck Hankla, Taradon Luangtongkum
The Thai Journal of Veterinary Medicine
Campylobacteriosis, one of the most common food-borne diseases in humans, is mainly caused by the consumption of chicken meat contaminated with Campylobacter. In this study, we examined the recovery rate of Campylobacter from carcass rinse using direct plating method and selective enrichment method. Campylobacter genotypes obtained from both methods were also determined using flaA-short variable region (flaA-SVR) sequencing. Results showed that Campylobacter recovery rates by the direct plating method and selective enrichment method were 40.00% and 46.86%, respectively. However, when a combination of the two methods was used, the recovery rate increased to 64.57%. For genetic characterization of Campylobacter, 9 …
Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study,
2016
Chulalongkorn University
Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta
The Thai Journal of Veterinary Medicine
Involucrin (INV) is a cornified envelope precursor protein that is commonly used as an early stage marker of human terminal keratinocyte differentiation. This research investigated the expression of INV in canine skin tumors using 56 formalin-fixed paraffin-embedding (FFPE) biopsied specimens. The expression of INV was investigated by immunohistochemistry (IHC) and RT-PCR. Correlation between INV and Ki-67 was also performed. The malignant tumor groups showed significantly different INV staining scores and Ki-67 positive cells than the normal skin group by IHC (p<0.05). The INV scores of moderately differentiated SCC (MSCC), trichoblastoma (TCB), basal cell carcinoma (BCC) and poorly differentiated SCC (PSCC) significantly decreased, with a statistically significant difference (p<0.05). Interestingly, the INV of poorly differentiated SCC disappeared in some specimens. The INV expression in most tumors was found to be inversely proportionate to Ki-67 positive cells. In addition, only SCC regardless of tumor grade without tumor-graded specimens showed an inversely statistically significant difference between the Ki-67 positive cells and INV expression (p<0.0001). In RT-PCR, mRNA expression of the FFPE specimens showed substantial similarity of INV expression as in IHC. It is suggested that the INV expression should be further studied in terms of clinical prognosis in canine cutaneous SCC.
Mechanical Conflict System: A Novel Operant Method For The Assessment Of Nociceptive Behavior,
2016
University of Michigan - Ann Arbor
Mechanical Conflict System: A Novel Operant Method For The Assessment Of Nociceptive Behavior, Steven E. Harte, Jessica B. Meyers, Renee R. Donahue, Bradley K. Taylor, Thomas J. Morrow
Physiology Faculty Publications
A new operant test for preclinical pain research, termed the Mechanical Conflict System (MCS), is presented. Rats were given a choice either to remain in a brightly lit compartment or to escape to a dark compartment by crossing an array of height-adjustable nociceptive probes. Latency to escape the light compartment was evaluated with varying probe heights (0, .5, 1, 2, 3, and 4 mm above compartment floor) in rats with neuropathic pain induced by constriction nerve injury (CCI) and in naive control rats. Escape responses in CCI rats were assessed following intraperitoneal administration of pregabalin (10 and 30 mg/kg), morphine …
Taylor, Charles Forman, Jr., 1862-1946 (Sc 2975),
2016
Western Kentucky University
Taylor, Charles Forman, Jr., 1862-1946 (Sc 2975), Manuscripts & Folklife Archives
Manuscript Collection Finding Aids
Finding aid and scan (Click on "Additional Files" below) for Manuscripts Small Collection 2975. Letter, 21 October 1939, written by druggist Charles Forman Taylor Jr., Bowling Green, Kentucky to Joe Werthan, Nashville, Tennessee, describing a cough syrup to be used on Mr. Werthan’s horses. The letter includes a list of Taylor’s other equine medications with prices.
Evaluation Of Calving Indicators Measured By Automated Monitoring Devices To Predict The Onset Of Calving In Holstein Dairy Cows,
2016
Université Laval
Evaluation Of Calving Indicators Measured By Automated Monitoring Devices To Predict The Onset Of Calving In Holstein Dairy Cows, V. Ouellet, E. Vasseur, W. Heuwieser, O. Burfeind, X. Maldague, É. Charbonneau
Physiology Collection
Dystocias are common in dairy cows and often adversely affect production, reproduction, animal welfare, labor, and economics within the dairy industry. An automated device that accurately predicts the onset of calving could potentially minimize the effect of dystocias by enabling producers to intervene early. Although many well-documented indicators can detect the imminence of calving, research is limited on their effectiveness to predict calving when measured by automated devices. The objective of this experiment was to determine if a decrease in vaginal temperature (VT), rumination (RT), and lying time (LT), or an increase in lying bouts (LB), as measured by …
The Effects Of Acidified Pepsin On Porcine Vocal Fold Tissue: Developing A Porcine Model Of Laryngopharyngeal Reflux Disease,
2016
Purdue University
The Effects Of Acidified Pepsin On Porcine Vocal Fold Tissue: Developing A Porcine Model Of Laryngopharyngeal Reflux Disease, Abigail Cox Durkes
Open Access Dissertations
Approximately 7.5 million Americans are affected by a voice disorder. In the last year, 7.2% of people missed one or more days of work due to a voice problem. For professional voice users, such as teachers, the rate increases to 20% and an annual cost of $2.5 billion. Voice disorders are complex and multi-faceted, as well as difficult to diagnose and treat. Prospective animal studies are necessary to study the pathophysiology of voice disorders, optimize our understanding of laryngeal pathology, and improve treatment outcomes. The pig provides a unique opportunity to test hypotheses relating to laryngeal disease because porcine vocal …
The Pseudorabies Virus Protein, Pul56, Enhances Virus Dissemination And Virulence But Is Dispensable For Axonal Transport,
2016
Northwestern University
The Pseudorabies Virus Protein, Pul56, Enhances Virus Dissemination And Virulence But Is Dispensable For Axonal Transport, Gina R. Daniel, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith
School of Veterinary and Biomedical Sciences: Faculty Publications
Neurotropic herpesviruses exit the peripheral nervous system and return to exposed body surfaces following reactivation from latency. The pUS9 protein is a critical viral effector of the anterograde axonal transport that underlies this process. We recently reported that while pUS9 increases the frequency of sorting of newly assembled pseudorabies virus particles to axons from the neural soma during egress, subsequent axonal transport of individual virus particles occurs with wild-type kinetics in the absence of the protein. Here, we examine the role of a related pseudorabies virus protein, pUL56, during neuronal infection. The findings indicate that pUL56 is a virulence factor …
Local Admixture Of Amplified And Diversified Secreted Pathogenesis Determinants Shapes Mosaic Toxoplasma Gondii Genomes,
2016
J. Craig Venter Institute
Local Admixture Of Amplified And Diversified Secreted Pathogenesis Determinants Shapes Mosaic Toxoplasma Gondii Genomes, Hernan Lorenzi, Asis Khan, Michael S. Behnke, Sivaranjani Namasivayam, Lakshmipuram S. Swapna, Michalis Hadjithomas, Svetlana Karamycheva, Deborah Pinney, Brian P. Brunk, James W. Ajioka, Daniel Ajzenberg, John C. Boothroyd, Jon P. Boyle, Marie L. Dardé, Maria A. Diaz-Miranda, Jitender P. Dubey, Heather M. Fritz, Solange M. Gennari, Brian D. Gregory, Kami Kim, Jeroen P. J. Saeij, Chunlei Su, Michael W. White, Xing-Quan Zhu, Daniel K. Howe, Benjamin M. Rosenthal, Michael E. Grigg, John Parkinson, Liang Liu, Jessica C. Kissinger
Veterinary Science Faculty Publications
Toxoplasma gondii is among the most prevalent parasites worldwide, infecting many wild and domestic animals and causing zoonotic infections in humans. T. gondii differs substantially in its broad distribution from closely related parasites that typically have narrow, specialized host ranges. To elucidate the genetic basis for these differences, we compared the genomes of 62 globally distributed T. gondii isolates to several closely related coccidian parasites. Our findings reveal that tandem amplification and diversification of secretory pathogenesis determinants is the primary feature that distinguishes the closely related genomes of these biologically diverse parasites. We further show that the unusual population structure …
Noninvasive Assessment Of Cardiac Abnormalities In Experimental Autoimmune Myocarditis By Magnetic Resonance Microscopy Imaging In The Mouse,
2016
University of Nebraska-Lincoln
Noninvasive Assessment Of Cardiac Abnormalities In Experimental Autoimmune Myocarditis By Magnetic Resonance Microscopy Imaging In The Mouse, Chandirasegaran Massilamany, Vahid Khalilzad-Sharghi, Arunakumar Gangaplara, David Steffen, Shadi F. Othman, Jay Reddy
Jay Reddy Publications
Myocarditis is an inflammation of the myocardium, but only ~10% of those affected show clinical manifestations of the disease. To study the immune events of myocardial injuries, various mouse models of myocarditis have been widely used. This study involved experimental autoimmune myocarditis (EAM) induced with cardiac myosin heavy chain (Myhc)-α 334-352 in A/J mice; the affected animals develop lymphocytic myocarditis but with no apparent clinical signs. In this model, the utility of magnetic resonance microscopy (MRM) as a non-invasive modality to determine the cardiac structural and functional changes in animals immunized with Myhc-α 334-352 is shown. EAM and healthy mice …
TÜBİTAK
Research Of The Effects Of Autologous Cancellous Bone Graft And Hyaluronic Acidon The Healing Of Bone Defects Experimentally Induced In Rabbits, Aydin Sağliyan, Mehmet Cengi̇z Han, Eni̇s Karabulut, Mustafa Özkaraca
Turkish Journal of Veterinary & Animal Sciences
The present study investigated the effect of autograft and hyaluronic acid (HA) in terms of clinical, radiological, and histopathological aspects on the healing of experimentally induced bone defects. The animals (n = 42) were assorted into 3 groups. After general anesthesia, a 3-mm standard defect was formed from the tibia of the experimental animals. The defects in Group I (n = 14) were left blank. Cancellous grafts in the proximal tibia were collected using a small curette to fill the defects in Group II (n = 14). The defects in Group III (n = 14) were filled by mixing with …
Exploring Attitudes Toward Euthanasia Among Shelter Workers And Volunteers In Japan And The U.S.,
2016
Humane Society University
Exploring Attitudes Toward Euthanasia Among Shelter Workers And Volunteers In Japan And The U.S., Maho Cavalier
HSU STUDENT THESES AND CAPSTONE PROJECTS
Conflicts between foreign and Japanese volunteers concerning decisions to euthanize animals that are in terminal condition were noted during anecdotal observation by this author at a Japanese animal rescue shelter. Thus, this research was undertaken to explore attitudes of shelter staff in Japan. As a point of comparison, U. S. shelter workers were also interviewed. Twenty current or former shelter workers (16 participants from Japan and 4 participants from the U.S.), ranging in age from 20 to 65, were interviewed. Face-to-face interviews were conducted with the Japanese participants; e-mail and/or Skype were used to conduct the interviews with the participants …
Dry Period Plane Of Energy: Effects On Glucose Tolerance In Transition Dairy Cows,
2016
Cornell University
Dry Period Plane Of Energy: Effects On Glucose Tolerance In Transition Dairy Cows, S. Mann, F. A. Leal Yepes, M. Duplessis, J. J. Wakshlag, T. R. Overton, B. P. Cummings, D. V. Nydam
Physiology Collection
Overfeeding energy in the dry period can affect glucose metabolism and the energy balance of transition dairy cows with potential detrimental effects on the ability to successfully adapt to early lactation. The objectives of this study were to investigate the effect of different dry cow feeding strategies on glucose tolerance and on resting concentrations of blood glucose, glucagon, insulin, nonesterified fatty acids (NEFA), and β-hydroxybutyrate (BHB) in the peripartum period. Cows entering second or greater lactation were enrolled at dry-off (57 d before expected parturition) into 1 of 3 treatment groups following a randomized block design: cows …
Body Condition Score And Plane Of Nutrition Prepartum Affect Adipose Tissue Transcriptome Regulators Of Metabolism And Inflammation In Grazing Dairy Cows During The Transition Period,
2016
University of Illinois at Urbana-Champaign
Body Condition Score And Plane Of Nutrition Prepartum Affect Adipose Tissue Transcriptome Regulators Of Metabolism And Inflammation In Grazing Dairy Cows During The Transition Period, M. Vailati-Riboni, M. Kanwal, O. Bulgari, S. Meier, N. V. Priest, C. R. Burke, J. K. Kay, S. Mcdougall, M. D. Mitchell, C. G. Walker, M. Crookenden, A. Heiser, J. R. Roche, J. J. Loor
Physiology Collection
Recent studies demonstrating a higher incidence of metabolic disorders after calving have challenged the management practice of increasing dietary energy density during the last ~3 wk prepartum. Despite our knowledge at the whole-animal level, the tissue-level mechanisms that are altered in response to feeding management prepartum remain unclear. Our hypothesis was that prepartum body condition score (BCS), in combination with feeding management, plays a central role in the peripartum changes associated with energy balance and inflammatory state. Twenty-eight mid-lactation grazing dairy cows of mixed age and breed were randomly allocated to 1 of 4 treatment groups in a 2 × …
Table Of Contents 2016 Nebraska Beef Cattle Report,
2016
University of Nebraska - Lincoln
Table Of Contents 2016 Nebraska Beef Cattle Report
Nebraska Beef Cattle Reports
No abstract provided.
Cobalt Use And Regulation In Horseracing: A Review,
2016
University of Florida
Cobalt Use And Regulation In Horseracing: A Review, K. Brewer, G. Maylin, C. K. Fenger, Thomas Tobin
Veterinary Science Faculty Publications
Cobalt, atomic weight 58.9, is a metallic element and environmental substance found in the animal in microgram quantities, predominantly as vitamin B12, but is also a component of at least one mammalian enzyme unassociated with B12. Cobalt is a required trace mineral and has long been administered as a dietary supplement to humans and animals. Cobalt deficiency outside of its requirement in vitamin B12 has not been reported in humans. The administration of cobalt salts was once standard treatment for anaemia in humans, owing to its ability to stimulate red blood cell synthesis. Elemental cobalt acts by stabilising hypoxia inducible …
Development And Characterization Of A Recombinant Orf Virus Vector Expressing The Spike Protein Of Porcine Epidemic Diarrhea Virus,
2016
South Dakota State University
Development And Characterization Of A Recombinant Orf Virus Vector Expressing The Spike Protein Of Porcine Epidemic Diarrhea Virus, Kyle Hain
Electronic Theses and Dissertations
Orf virus (ORFV), the type member of the genus Parapoxvirus of the family Poxviridae, causes orf or cutaneous pustular dermatitis in sheep and goats. ORFV is a ubiquitous virus capable of re-infecting its hosts multiple times over time. ORFV causes a non-systemic, self-limiting disease which is usually restricted to the skin surrounding the virus entry sites. ORFV has evolved several immunomodulatory proteins (IMPs) that evade and/or modulate host immune responses to infection and contribute to virus virulence and disease pathogenesis. Given biological properties and unique immunomodula tor y properties, ORFV has gained significant attention in recent years for its …
