Veterinary Medicine Commons^™

Comparison Of Protein Phosphatase Inhibition Assay With Lc-Ms/Ms For Diagnosis Of Microcystin Toxicosis In Veterinary Cases, Caroline E. Moore, Jeanette Juan, Yanping Lin, Cynthia L. Gaskill, Birgit Puschner

Veterinary Diagnostic Laboratory Faculty Publications

Microcystins are acute hepatotoxins of increasing global concern in drinking and recreational waters and are a major health risk to humans and animals. Produced by cyanobacteria, microcystins inhibit serine/threonine protein phosphatase 1 (PP1). A cost-effective PP1 assay using p-nitrophenyl phosphate was developed to quickly assess water and rumen content samples. Significant inhibition was determined via a linear model, which compared increasing volumes of sample to the log-transformed ratio of the exposed rate over the control rate of PP1 activity. To test the usefulness of this model in diagnostic case investigations, samples from two veterinary cases were tested. In August …

Analysis Of Johne's Disease Elisa Status And Associated Performance Parameters In Irish Dairy Cows, A. E. Kennedy, N. Byrne, A. B. Garcia, Jim O'Mahony, R. G. Sayers

Department of Biological Sciences Publications

Background

Infection with Mycobacterium avium subspecies paratuberculosis (MAP) has been associated with reductions in milk production in dairy cows and sub optimal fertility. The aim of this study was to highlight the production losses associated with testing MAP ELISA positive in Irish dairy cows. Secondary objectives included investigation of risk factors associated with testing MAP ELISA positive. A survey of management practices on study farms was also conducted, with examination of associations between management practices and herd MAP status.

Blood samples were collected from 4188 breeding animals on 22 farms. Samples were ELISA tested using the ID Screen Paratuberculosis Indirect …

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1 VBSD/ADRDL Researchers Benefit from new Oscar Olson Research Laboratories at SDSU
[Page] 2 Director's Message: The ADRDL in State Statute
[Page] 3 Darrell Johnson Honored by Department
[Page] 3 Pork Quality Assurance Advisor Training Scheduled at SDSU
[Page] 4 Specimen Receiving and Necropsy Sections at the ADRDL: Getting the Diagnostic Process off to a Great Start
[Page] 6 New Anaplasmosis PCR test online at ADRDL
[Page] 6 Laura Schanning Joins ADRDL in Specimen Receiving
[Page] 6 Reminder: Case History Helps Calf Scours Diagnosis
[Page] 6 Dr. Beverly Shelbourn Joins Veterinary and Biomedical Sciences Department as Instructor
[Page] 6 …

Circulating Amino Acids In Blood Plasma During The Peripartal Period In Dairy Cows With Different Liver Functionality Index, Z. Zhou, J. J. Loor, F. Piccioli-Cappelli, F. Librandi, G. E. Lobley, E. Trevisi

Physiology Collection

The liver functionality index (LFI) measures the changes of albumin, cholesterol, and bilirubin concentrations between 3 and 28 d postpartum. This composite index, based on variables with direct relevance to liver-specific plasma protein synthesis (albumin), hepatic/intestinal lipoprotein synthesis (cholesterol), and clearance of breakdown products of heme catabolism (bilirubin), provides a tool for evaluating manifestations of hepatic disease. Both energy and protein metabolism are likely to be affected by various physiological challenges in this period but have not been tested systematically. The present study was conducted to profile AA in cows with high or low LFI during the peripartal period …

The Effect Of Various Erythrocyte Species On The Detection Of Avian, Swine And Canine Influenza A Viruses Isolated In Thailand, Aunyaratana Thontiravong, Duangduean Prakairungnamthip, Sunicha Chanvatik, Nutthawan Nonthabenjawan, Wikanda Tunterak, Ratanaporn Tangwangvivat, Kanisak Oraveerakul, Alongkorn Amonsin

The Thai Journal of Veterinary Medicine

Hemagglutination (HA) and hemagglutination inhibition (HI) tests are conventional, well-known methods used for the detection of influenza A viruses (IAVs) and antibodies to IAVs, respectively. The sensitivity of HA and HI tests is usually affected by the compatibility of sialic acid (SA) receptors on red blood cells (RBCs) with the HA proteins of IAVs. In this study, the erythrocyte binding preferences of sixteen avian, swine and canine IAVs from Thailand were investigated using five erythrocyte sources: chicken, turkey, goose, guinea pig and horse RBCs. The results demonstrated that, turkey RBCs yielded the highest HA titers against most avian, swine and …

Control Of Estrus In Gilts And Primiparous Sows, Roy N. Kirkwood, Fabio De Rensis

The Thai Journal of Veterinary Medicine

The primary controller of weaned pig output is the successful breeding of enough females in the breeding week. The number of females available for breeding is dependent on the number of sows weaned sows and service-ready gilt availability. Primiparous (P1) sows often have longer wean-estrus intervals (WEI) and increased anestrus; ensuring sufficient gilts and sows are available may require control of estrus. Stimulating estrus requires appropriate boar contact or when this is not sufficient, administration of gonadotrophins. If gonadotrophins are used expect estrus 4 to 6 d later and it is usual to breed at the induced estrus. If there …

Comparison Of Effects Of Different Antioxidants Supplemented To Long-Term Extender On Boar Semen Quality Following Storage At 17ºc, Thevin Vongpralub, Pachara Thananurak, Chamaiporn Sittikasamkit, Phirawit Chuawongboon, Monchai Duangjinda, Wuttigrai Boonkum, Vibuntita Chankitisakul

The Thai Journal of Veterinary Medicine

The use of antioxidants as semen additives in the extender has been proposed to improve sperm function during cool storage. The objective of this study was to compare the effects of different antioxidant supplementation in extender on boar semen quality during storage for 10 days at 17ºC. Semen samples (n=18) were divided into 10 aliquots and diluted with Reading extender supplemented with three different concentrations of water-soluble glutathione, L-carnitine, and vitamin E analogue Trolox (0.1, 1 and 5mM). All samples were stored in a refrigerator at 17ºC for 10 days. Sperm motility was assessed at Days 1, 4, 7 and …

Evaluation Of Amelogenin And Zinc-Finger Loci For Sex Identification In Captive Felids, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

The amelogenin and zinc-finger regions were used for sex determination in six species of felids: Felis catus (Persian cat), Felis chaus (jungle cat), Prionailurus bengalensis (leopard cat), Pardofelis temminckii (Asiatic golden cat), Panthera tigris (tiger) and Prionailurus viverrinus (fishing cat). The amelogenin region in all six felids could be successfully amplified and gave a heterozygous genotype in males and a homozygous genotype in females, except for leopard cats which showed a heterozygous genotype in both sexes. The PCR products from the zinc-finger region could be amplified in Persian cats, Asiatic golden cats, tigers and fishing cats. In the Persian cat, …

Cisplatin Intralesional Chemotherapy Of Recurrence Schwannoma In Horse: A Case Report, Rungrueang Yodsheewan, Kanittha Phetudomsinsuk

The Thai Journal of Veterinary Medicine

A 7-year-old crossbred stallion had multiple solitary cutaneous masses at the right infraorbital area which were recurrent for two months. The biggest mass size was 5 x 7 x 1 cm3 and had superficial bleeding. This case was diagnosed as peripheral nerve sheath tumors based on cytological and histological examination which showed numerous spindle cells with cancerous characteristics, cell patterns of the tumor were interlacing bundle and a whirl. Most areas showed tense palisading nuclei of tumor cells (Verocay bodies) known as Antoni A area. The rest showed loose paucicellular tissue known as Antoni B area. Within histologic sections, the …

Effect Of Antibiotic Growth Promoters On Anti-Oxidative And Anti-Inflammatory Activities In Broiler Chickens, Krittika Kabploy, Nuntavan Bunyapraphatsara, Noppawan Phumala Morales, Nuanchan Paraksa

The Thai Journal of Veterinary Medicine

The current study was conducted to evaluate the effects of sub-therapeutic antibiotics (avilamycin and flavophospholipol) in animal feed on anti-oxidative and anti-inflammatory properties in broilers to understand the mechanisms of AGPs. Four hundred and fifty six 1-day-old broilers (ROSS 308) were divided into 3 groups with 4 replications. Three experimental diets composed of a corn-soybean meal based diet supplemented with 0, 10 mg/kg avilamycin and 5 mg/kg flavophospholipol were randomly provided to the broilers for 42 days, respectively. Results showed that the AGPs significantly decreased TBA-MDA adduct in serum as well as villi tissue of the broilers, especially flavophospholipol. However, …

Study Of Genetic Variation Of Captive Asiatic Golden Cat (Pardofelis Temminckii) In Thailand Using Domestic Cat (Felis Catus) Microsatellite Markers, Suwimon Phandee, Janjira Phavaphutanon, Kaikanoke Sirinarumitr, Sudtisa Laopiem, Theerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

Domestic cat (Felis catus) microsatellite markers were used to determine genetic diversity in 17 captive Asiatic golden cats (Pardofelis temminckii, syn. Catopuma temminckii). Allele numbers, genotype numbers, genetic diversity, heterozygosity, polymorphism information content (PIC) and inbreeding coefficient (f) were observed in each marker. The average values of genotype number, allele number, expected heterozygosity, observed heterozygosity, polymorphism information content (PIC) and inbreeding coefficient (f) were 6.12, 4.71, 0.70, 0.29, 0.65 and 0.62, respectively. These results revealed a reduction in the genetic diversity as shown by the lower value for the observed heterozygosity compared to the expected heterozygosity and the high value …

Aflatoxin Effect On Humoral And Mucosal Immune Responses Against Infectious Bronchitis Vaccine In Broilers, Babak Shahabi-Ghahfarokhi, Majid Gholami-Ahangaran, Mohsen Jafarian-Dehkordi

The Thai Journal of Veterinary Medicine

The aflatoxin effect on humoral and mucosal immunity against infectious bronchitis (IB) vaccine was studied in 225 one-day-old chicks. The chicks were divided in 3 equal groups. Treatment groups included group A: chickens fed a basal diet, and group B: chickens fed 3 ppm productive aflatoxin in a basal diet. The chickens in groups A and B were vaccinated against IB (at one and 10 days old) by H120 live attenuated vaccine. For group C, chickens did not receive aflatoxin and were not vaccinated against IB. All chickens received experimental diets from 3 to 28 days old, continuously. At 28 …

Foal Deciduous Teeth Stem Cells Enhance Wound Healing In Rabbit Wound Model, Nitipon Srionrod, Ratikorn Bootcha, Soontaree Petchdee

The Thai Journal of Veterinary Medicine

The purpose of this study was to evaluate the potential use of deciduous teeth stem cells from horses for wound healing in animal wound model. This study assessed the efficacy and safety of foal deciduous teeth stem cells (fDSCs) administration in rabbit excisional wound model. Deciduous teeth stem cells were harvested from horses and full thickness excisional wound was made on rabbits by surgical removal of epidermis and dermis to the depth of subcutaneous fat. New Zealand White rabbits (n=8) were divided into a control group (n=4) and a treatment group (n=4). Multiple injections of fDSCs suspension were intravenously administered …

Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Sirilak Disatian Surachetpong, Pakit Boonpala

The Thai Journal of Veterinary Medicine

No abstract provided.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Application Of Real-Time Pcr For Quantifying Gastrointestinal Microbiota In Weaning Pigs Influenced By Dietary Feed Additive Supplementation, Phattarapon Chaloemnon, Mullika Traidej Chomnawang, Wirawan Junlapho, Nuanchan Paraksa

The Thai Journal of Veterinary Medicine

To apply real-time polymerase chain reaction (PCR) to detect and quantify the gastrointestinal microbiota modulated by dietary feed additives was the objective of this study. Sixty-four three-crossbred weaning piglets at 24 days were used and randomly assigned to be fed one of the experimental diets supplemented with different feed additives (0.2% inulin, 0.1% fructooligosaccharide and sub-therapeutic antibiotics), and compared with the non-supplemented group. Comparison of microbiota enumeration by conventional culture and real-time PCR methods in response to feed additives was investigated. The microbiota enumeration by conventional culture and real-time PCR methods showed corresponding responses of selected bacterial counts (Lactobacilli, Bifidobacteria, …

Use Of Different Models For Estimation Of Genetic Parameters And Genetic Trends Of Performance Test Traits Of Gilts, Dragomir Lukač

The Thai Journal of Veterinary Medicine

The aims of this research were to compare estimates of variance components using different animal models and to determine the most suitable mixed model for estimating genetic parameters and genetic trends for traits in performance test of gilts of different breeds using REML. A total of 73129 gilts of four genotypes in the period of 2009 to 2013 were included in the analyses. Four mixed models were constructed. Information criterion of Akaike (AIC) and Bayesian information criterion (BIC) were used to suggest which model is an adequate model for evaluation of genetics parameters. With the introduction of certain factors in …

Prevalence Of Subclinical Laminitis And Its Effects On Reproductive Performance In Lactating Cows In Thailand, Suvaluk Seesupa, Kwankate Kanistanon, Rittichai Pilachai, Suneerat Aiumlamai

The Thai Journal of Veterinary Medicine

The objectives of this study were to investigate the prevalence of subclinical laminitis and to evaluate its effects on reproductive performances in dairy cows in Thailand. Ninety-eight lactating cows from 22 smallholder dairy farms and 138 lactating cows from one large scale dairy farm were assessed for subclinical laminitis by evaluating lesions after hoof trimming. Hemorrhagic lesions of the sole and white line area were evaluated. Any appearance of sole hemorrhage or white line hemorrhage scoring 2 on one or more claws was defined as a case of subclinical laminitis. Reproductive data were recorded and analyzed using subclinical laminitis as …

Activated Partial Thromboplastin Time (Aptt) In Dogs Tested By In-House Crude Cephalin From Canine Brain, Sarawut Yama, Am Chaimongkhol, Somsak Channoi, Sakhorn Pornprasert, Wanna Suriyasathaporn

The Thai Journal of Veterinary Medicine

This research aimed to identify the reference value of activated partial thromboplastin time (APTT) in normal dogs using crude cephalin prepared in-house from canine brain, and to examine its efficacy in detecting disorders of coagulation factors. A total of 105 blood samples were collected from normal dogs between January-May 2010. The APTT value was found to be 10.43-19.08 seconds and significantly correlated with the APTT value of a test using a commercial reagent (Pearson’s correlation, r = 0.9923, p < 0.001). On testing the reagent’s efficacy in detecting disorders of coagulation factors by using cryopoor plasma, it was discovered that the APTT value ranged from 24.50 to 33.44 seconds, and the mean value of the APTT by means of cryopoor plasma was significantly greater than that of normal dogs (p < 0.001). The aforementioned APTT value significantly correlated with that obtained by means of a commercial reagent (Pearson’s correlation, r = 0.9984, p < 0.001). From this research, it can be confirmed that in-house crude cephalin can be substituted for commercial crude cephalin. Besides, the obtained reference range can further be applied in veterinary hematology laboratory.

Comparison Of Two Methods For The Recovery Of Campylobacter From Chicken Carcasses, Suthida Muangnoicharoen, Sakaoporn Prachantasena, Luck Hankla, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

Campylobacteriosis, one of the most common food-borne diseases in humans, is mainly caused by the consumption of chicken meat contaminated with Campylobacter. In this study, we examined the recovery rate of Campylobacter from carcass rinse using direct plating method and selective enrichment method. Campylobacter genotypes obtained from both methods were also determined using flaA-short variable region (flaA-SVR) sequencing. Results showed that Campylobacter recovery rates by the direct plating method and selective enrichment method were 40.00% and 46.86%, respectively. However, when a combination of the two methods was used, the recovery rate increased to 64.57%. For genetic characterization of Campylobacter, 9 …