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Knowledge, Attitude And Practice Associated With Brucellosis In Occupational Risk Groups In China, Quangang Xu 2021 Faculty of Veterinary Science

Knowledge, Attitude And Practice Associated With Brucellosis In Occupational Risk Groups In China, Quangang Xu

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to analyze the situation of knowledge, attitude, and practice (KAP) of sheep and goat farmers and community animal health staff towards the prevention and control of brucellosis. A total of 1,067 sheep and goat farmers and 401 community animal health staff from seven provinces in China were participated in this study from 2017 to 2018. Two structured questionnaire were used to examine demographic characteristics and KAP related to brucellosis. Descriptive statistics were used for demographic characteristics and KAP. Mann-Whitney U test was used to analyze the potential risk factors associated with KAP among participants. The overall awareness …


Selection Of Candidate Probiotic Bacteria From Chickens With Antagonism Against Campylobacter, Sirawich Danwanichwong 2021 Faculty of Veterinary Science

Selection Of Candidate Probiotic Bacteria From Chickens With Antagonism Against Campylobacter, Sirawich Danwanichwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Campylobacter is one of the most common causes of bacterial foodborne disease in humans worldwide. The effect of probiotics on Campylobacter control in poultry is inconsistent or rarely observed. For this reason, research on probiotics for Campylobacter control in poultry is required. Therefore, the objective of this study was to identify lactobacilli, Bacillus or Enterococcus strains isolated from Campylobacter-negative chickens which have the ability to inhibit the growth of Campylobacter in vitro and demonstrate favorable probiotic characteristics. A total of 602 bacterial isolates from Campylobacter-negative broiler chickens were assessed for their probiotic properties including Campylobacter inhibiting activity test, hemolytic activity …


In Vitro Inhibitory Effect Of Lactic Acid Bacteria And Their Supernatants On Flavobacterium Columnare Biofilm, Farras Daffa Imtiyaz 2021 Faculty of Veterinary Science

In Vitro Inhibitory Effect Of Lactic Acid Bacteria And Their Supernatants On Flavobacterium Columnare Biofilm, Farras Daffa Imtiyaz

Chulalongkorn University Theses and Dissertations (Chula ETD)

Flavobacterium columnare is an opportunistic bacterium causing columnaris disease to more than 37 fish species. It is believed that the bacteria form biofilm on the fish skin/surfaces before the bacteria in the biofilm produce and release enzymes that degrade connective and other tissues resulting in lesions on the infected fish. Treatments to inhibit the bacterial biofilm without interfering with its planktonic cell growth can be an alternative method to control columnaris disease. This study aimed to evaluate the efficacy of lactic acid bacteria (LAB) isolated from white leg shrimp gut (Penaeus vannamei) and the root of Jerusalem artichoke (Helianthus tuberosus) …


Prevalence And Molecular Characteristics Of Antimicrobial Resistance Of Aeromonas Hydrophila, Salmonella Spp., Vibrio Cholerae, Fecal Coliform, And Escherichia Coli In Hybrid Red Tilapia And Cultured Water, Varangkana Thaotumpitak 2021 Faculty of Veterinary Science

Prevalence And Molecular Characteristics Of Antimicrobial Resistance Of Aeromonas Hydrophila, Salmonella Spp., Vibrio Cholerae, Fecal Coliform, And Escherichia Coli In Hybrid Red Tilapia And Cultured Water, Varangkana Thaotumpitak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Foodborne pathogens and antimicrobial resistant bacteria in tilapia and cultivation water has been emerged as a public health threat. This study aimed to determine the prevalence of indicator and pathogenic bacteria in hybrid red tilapia (Oreochromis spp.) and their cultivation water, and to characterize phenotypic and genotypic AMR, virulence genes, and extended-spectrum beta-lactamase (ESBL) production of Escherichia coli, Aeromonas hydrophila, Salmonella spp., and Vibrio cholerae. A total of 120 tilapia, which were comprised of carcass rinse (n=120), muscle (n=120), intestine (n=120), liver and kidney (n=120), and cultivation water (n=120) were collected from tilapia farms in Kanchanaburi province, Thailand during October …


Genetic Characterizations, Virulence, Antimicrobial Resistances And Resistome Analysis Of Vibrio Spp. Isolated From Farmed Asian Sea Bass (Lates Calcarifer) In Thailand, Hartanto Mulyo Raharjo 2021 Faculty of Veterinary Science

Genetic Characterizations, Virulence, Antimicrobial Resistances And Resistome Analysis Of Vibrio Spp. Isolated From Farmed Asian Sea Bass (Lates Calcarifer) In Thailand, Hartanto Mulyo Raharjo

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study describes the etiological agent of Vibriosis along with its distribution and antimicrobial resistance profiles among farmed Asian sea bass (Lates calcarifer) in Thailand. The study isolated 283 Vibrionaceae from 15 Asian sea bass farms located around the provinces of the Andaman Sea and Gulf of Thailand coasts to uncover the distribution and antimicrobial resistance profiles. Bacterial identification based on a combination of the biochemical characteristics, Matrix-assisted laser desorption/ ionization-time of flight (MALDI-TOF) mass spectrometry (MS) analysis, and the species-specific PCR demonstrated the predominant Vibrionaceae were Vibrio harveyi (n = 56), Photobacterium damselae (n = 35), and V. vulnificus …


Identification, Characterization And Pathogenicity Of Aeromonas Schubertii Isolated From Diseased Striped Snakehead Fish (Channa Striata), Hendri Budiyansah 2021 Faculty of Veterinary Science

Identification, Characterization And Pathogenicity Of Aeromonas Schubertii Isolated From Diseased Striped Snakehead Fish (Channa Striata), Hendri Budiyansah

Chulalongkorn University Theses and Dissertations (Chula ETD)

Striped snakehead fish (Channa striata) is one of the popular cultivated fish in Thailand. However, it is inevitable to be susceptible to abundant pathogens, especially Aeromonas schubertii. In July 2020, an unknown outbreak was occurred on striped snakehead fish nursery farm in Nakhon Pathom province, Thailand. Suspected six isolates were A. schubertii yielded from the fish as the main causative agent. This research aimed to characterize and examine pathogenicity of A. schubertii isolated from diseased striped snakehead fish. Characterization was performed based on combination of biochemical assay, PCR and DNA sequencing (based on 16S rRNA and gyrB), and MALDI-TOF MS. …


Genome Analysis Of Predominant Aeromonas Species Isolated From Diseased Freshwater Fishes And Their Virulence In Striped Snakehead Fish (Channa Striata)||การวิเคราะห์จีโนมเชื้อแอโรโมนาสสปีชีส์เด่นที่แยกได้จากปลาน้ำจืดป่วยในประเทศไทยและความรุนแรงของเชื้อในปลาช่อนนา, Muhammad Fadhlullah Mursalim 2021 Faculty of Veterinary Science

Genome Analysis Of Predominant Aeromonas Species Isolated From Diseased Freshwater Fishes And Their Virulence In Striped Snakehead Fish (Channa Striata)||การวิเคราะห์จีโนมเชื้อแอโรโมนาสสปีชีส์เด่นที่แยกได้จากปลาน้ำจืดป่วยในประเทศไทยและความรุนแรงของเชื้อในปลาช่อนนา, Muhammad Fadhlullah Mursalim

Chulalongkorn University Theses and Dissertations (Chula ETD)

Aeromonas is an important bacterial pathogen in freshwater fishes. This study aimed to investigate the distribution and diversity of Aeromonas spp. including their antimicrobial resistance patterns. 86 Aeromonas isolates were collected from diseased fish in 13 fish farms around Thailand. Biochemical tests, MALDI-TOF MS, PCR assays, and the gyrB gene sequence analysis were used to identify all isolates. MALDI-TOF MS confirmed 100% (86 isolates) at the genus level and 88.4% (76 isolates) at the species level. Six Aeromonas species were confirmed based on sequences of gyrB gene, including A. veronii (72.1%), A. jandaei (11.6%), A. schubertii (9.3%), A. diversa (3.5%), …


Monitoring And Characterization Of Colistin Resistant Escherichia Coli In Pig Farm And Environment Following The Cessation In Use Of Colistin, Nwai Oo Khine 2021 Faculty of Veterinary Science

Monitoring And Characterization Of Colistin Resistant Escherichia Coli In Pig Farm And Environment Following The Cessation In Use Of Colistin, Nwai Oo Khine

Chulalongkorn University Theses and Dissertations (Chula ETD)

The growing cases of colistin resistance due to plasmid mediated mcr genes family in Enterobacteriaceae is catastrophic to public health. In this study, our team performed the nation-wide surveillance of mcr-positive Escherichia coli (MCRPE) in healthy pigs across Thailand. Then we monitored longitudinally over the representative pig farm after colistin was withdrawn and genomic characterization was carried out. Among the 696 samples collected from 49 provinces of Thailand, the low carriage rate of mcr-1 or combination of mcr-1 and mcr-3 (4.45% and 0.43%) were detected. MCRPE isolates were multidrug-resistant (MDR) against 3–14 types of antimicrobial and enterotoxin genes were largely …


Genetic Diversity And Distribution Of Tilapia Lake Virus In Fish Polyculture System In Bangladesh, Partho Pratim Debnath 2021 Faculty of Veterinary Science

Genetic Diversity And Distribution Of Tilapia Lake Virus In Fish Polyculture System In Bangladesh, Partho Pratim Debnath

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tilapia is the world's second most important farmed fish species, following carp. Bangladesh is the world's fourth-largest tilapia producer and has largely adopted fish polyculture. Few research on factors associated with mortality and economic losses following such deaths have been undertaken in Bangladesh due of its perceived hardiness. Tilapia lake virus (TiLV) is an emerging pathogen in aquaculture, reportedly affecting farmed tilapia in 16 countries across multiple continents. Following an early warning in 2017 that TiLV could be widespread, we surveyed 565 tilapia farms in 15 of Bangladesh's most important tilapia-producing districts using online tilapia epidemiology and health economics survey …


Anti-Inflammatory Efficacy Of New Zealand Green-Lipped Mussel Extract In Canine Chronic Stifle Osteoarthritis Monitoring By Thermographic Imaging, Supakit Vilaisuntornkiat 2021 Faculty of Veterinary Science

Anti-Inflammatory Efficacy Of New Zealand Green-Lipped Mussel Extract In Canine Chronic Stifle Osteoarthritis Monitoring By Thermographic Imaging, Supakit Vilaisuntornkiat

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to compare the anti-inflammatory effect of the NZGLM and placebo in patients with cranial cruciate ligament disease using thermographic imaging. This study evaluated the temperature from thermographic imaging to measure the reduce of inflammation. This study included 52 dogs with cranial cruciate disease and medial patellar luxation. All dogs were divided into 2 group that was group A and group B. The temperature at day 0 were 99.60 °F in group A and 99.07 °F in group B. The temperature at 4th week were 97.09 °F in group A and 99.02 °F in group B. The result …


Extending Animal Welfare Science To Include Wild Animals, Walter Veit, Heather Browning 2021 University of Sydney, Australia

Extending Animal Welfare Science To Include Wild Animals, Walter Veit, Heather Browning

Animal Sentience

Ng’s (2016) target article built on his earlier work advocating a science of welfare biology (Ng 1995). Although there were problems with the models proposed in Ng’s original paper regarding the balance of pleasure and suffering for wild animals, his call for a science of wild animal welfare was a sound one. This does not require a new discipline but just an extension of the existing frameworks and methods of animal welfare science to include wild animals.


Text Mining To Identify The Origin Of Chronic Wasting Disease, G. Webb 2021 San Jose State University

Text Mining To Identify The Origin Of Chronic Wasting Disease, G. Webb

Faculty Research, Scholarly, and Creative Activity

Chronic wasting  disease (CWD)  is  a 100  percent  fatal,  prion  disease of  deer  that  has  the potential  to decimate  the  deer  population and  jump to  the  human  population.   A  simple  Google  search on  the  “origin of  chronic wasting  disease” yielded  a total  of  56  relevant  articles.   Of  these,  75  percent  report  that  the origin  is  unknown,  19.6  percent  report  that  the  disease  may  have  originated  in  a  Fort  Collins,  Colorado, government  research facility, and 5.4 percent  report  other  possible  origins.  Government  sources  reported the  Fort  Collins  theory  4.3 percent  of  the  time  while  non-governmental  sources,  such  as news articles, reported …


Oviductal Cells Adhesion Test For Bull Semen Quality Assessment And Fertility Prediction Ofendangered Breeds: Proposal Of A Simplified Method, CALOGERO STELLETTA, KORAY TEKİN, BESTE ÇİL, JURI VENCATO, GIULIA GOLLIN, GIANCARLO BERTOLI, LUCILLO CESTARO, STEFANO ROMAGNOLI, ALİ DAŞKIN 2021 TÜBİTAK

Oviductal Cells Adhesion Test For Bull Semen Quality Assessment And Fertility Prediction Ofendangered Breeds: Proposal Of A Simplified Method, Calogero Stelletta, Koray Teki̇n, Beste Çi̇l, Juri Vencato, Giulia Gollin, Giancarlo Bertoli, Lucillo Cestaro, Stefano Romagnoli, Ali̇ Daşkin

Turkish Journal of Veterinary & Animal Sciences

Classical procedures for semen evaluation in breeding soundness examination require time and are costly. The evaluation techniques with CASA are limited due to the lack of standardization among laboratories and data analysis methods. Recent studies have contradictory conclusions on the actual predictive ability of this method alone regarding fertility. Finding a simple and objective test that can give the maximum correlation between in vitro and in vivo results has been the main focus of novel research. In this study, we aimed to develop a repeatable protocol of sperm adhesion test using oviductal explant (AOC) and the results were compared with …


Canine Hypoadrenocorticism: A Retrospective Study Of 32 Cases From 2011 To 2018., CINTHIA PERES CAMILO, MAURO JOSE LAHM CARDOSO, PAULA NASSAR DE MARCHI, RAFAEL FAGNANI 2021 TÜBİTAK

Canine Hypoadrenocorticism: A Retrospective Study Of 32 Cases From 2011 To 2018., Cinthia Peres Camilo, Mauro Jose Lahm Cardoso, Paula Nassar De Marchi, Rafael Fagnani

Turkish Journal of Veterinary & Animal Sciences

Primary hypoadrenocorticism is characterized by insufficient adrenocortical hormonal secretion of glucocorticoids and mineralocorticoids, which causes unspecific signs and laboratory alterations such as hyponatremia and hyperkalemia, resulting in a low relation between these two electrolytes. The aim of this work was to carry out a retrospective study with data obtained from the medical records of 32 dogs with a proven diagnosis of hypoadrenocorticism between the years 2011 and 2018. The parameters weight, sex, reproductive condition, breed, and age were analyzed, as well as the results of laboratory tests of dogs with confirmed hypoadrenocorticism. There was no breed or sex predisposition. There …


Caprine Mhc Gene Polymorphism And Its Association With Endoparasitic Infestation(Haemonchus Contortus) In Indian Goat Breeds, THIRUNAVUKKARASU SBALAMURUGAN, PUSHPENDRA KUMAR, KUSH SHRIVASTAVA, CHINMOY MISHRA, OM PRAKASH, AMIT KUMAR, ANUJ CHAUHAN, NIHAR RANJAN SAHOO, MANJIT PANIGRAHI, BHARAT BHUSHAN, ARVIND PRASAD, ILAYAKUMAR KAVERIYAPPAN, SHARAVANAN VELUSAMY 2021 TÜBİTAK

Caprine Mhc Gene Polymorphism And Its Association With Endoparasitic Infestation(Haemonchus Contortus) In Indian Goat Breeds, Thirunavukkarasu Sbalamurugan, Pushpendra Kumar, Kush Shrivastava, Chinmoy Mishra, Om Prakash, Amit Kumar, Anuj Chauhan, Nihar Ranjan Sahoo, Manjit Panigrahi, Bharat Bhushan, Arvind Prasad, Ilayakumar Kaveriyappan, Sharavanan Velusamy

Turkish Journal of Veterinary & Animal Sciences

The present study examined the variability of MHC class II DRB exon 2 gene using restriction fragment length polymorphism analysis of PCR-amplified fragments PCR-(RFLP), and its association with Haemonchus contortus infestation in Salem Black and Tellicherry goat population. Animals were naturally exposed to mixed infestation of endoparasites, predominantly Haemonchus contortus. Pooled fecal coproculture and larval identification showed predominant presence of haemonchus (L3) larva. Fecal egg count (FEC) and packed cell volume (PCV) were used as indicator traits. All the three studied loci, TaqI, BsaI, and BsaHI, were polymorphic having two alleles and three genotypes. The loci showed low to moderate …


The Effect Of Ellagic Acid On Performance, Digestibility, Egg Quality, Cecal Bacterial Flora,Antioxidant Activity, And Some Blood Parameters In Laying Quails Reared At Differenttemperatures, SEDA İFLAZOĞLU MUTLU, TALAT GÜLER 2021 TÜBİTAK

The Effect Of Ellagic Acid On Performance, Digestibility, Egg Quality, Cecal Bacterial Flora,Antioxidant Activity, And Some Blood Parameters In Laying Quails Reared At Differenttemperatures, Seda İflazoğlu Mutlu, Talat Güler

Turkish Journal of Veterinary & Animal Sciences

In this study, the effect of ellagic acid on the performance, egg quality, digestibility, cecal microflora, serum malondialdehyde (MDA) level, and biochemical parameters in quails reared under normal conditions and at high environmental temperatures was investigated. The animals (n = 240; 5-weeks old) reared at 2 environmental temperatures [thermoneutral (TN) and heat stress (HS)] and in 4 diet groups containing different ellagic acid doses (0, 100, 200, and 400 mg/kg) were randomly assigned to 8 groups according to the 2 × 4 factorial design. Feed intake, egg weight, and egg production were higher (P < 0.001) and feed conversion (P < 0.01) was better in the TN groups compared to the HS groups. Egg (P < 0.01) and shell weights (P < 0.001) were higher in the TN groups compared to the HS groups. The effect of ellagic acid supplementation on shell weight was statistically significant in the TN and HS groups (P < 0.05). The effects of environmental temperature and the supplementation of ellagic acid on digestibility of dry matter (P < 0.05), ether extract (P < 0.01), and crude protein (P < 0.01) were statistically significant in the TN and HS groups. Supplementation of ellagic acid reduced the total coliform bacteria count (P < 0.01) and increased the total lactic acid bacteria count (P < 0.001) in the cecum. Serum MDA levels increased with heat stress (P < 0.001) and decreased with the supplementation of ellagic acid (P < 0.001). The effect of environmental temperature on serum glucose (P < 0.001), cholesterol (P < 0.05), total protein (P < 0.05), alanine aminotransferase (ALT) (P < 0.001), sodium (P < 0.05), and magnesium was significant. In conclusion, the supplementation of ellagic acid decreased oxidative stress and total coliform bacteria count and improved the feed conversion and egg quality in quails under heat stress. Moreover, ellagic acid increased the digestibility of nutrients and had a positive effect on lactic acid bacteria. It was observed in this study that these beneficial effects of ellagic acid increased with an increasing dose, and 400 mg/kg was the most effective dose.


Determination Of Slaughter And Carcass Traits In Male Turkish Anatolian Buffaloes Atdifferent Slaughter Weights, ZAFER ULUTAŞ, AZİZ ŞAHİN, YÜKSEL AKSOY, EMRE UĞURLUTEPE 2021 TÜBİTAK

Determination Of Slaughter And Carcass Traits In Male Turkish Anatolian Buffaloes Atdifferent Slaughter Weights, Zafer Ulutaş, Azi̇z Şahi̇n, Yüksel Aksoy, Emre Uğurlutepe

Turkish Journal of Veterinary & Animal Sciences

Nowadays, deficits in red meat resources in Turkey are caused by the import of red meat from different countries. Anatolian buffaloes used in this research are a species that can contribute to red meat production in Turkey. This study was performed to determine the slaughter and carcass traits of Anatolian buffaloes at different slaughter weights. Twenty weaned male Anatolian buffalo calves of about 5 months old, at an average live weight of 100 kg, were used as the animal specimen in the present study. The calves were fed with 30:70 roughage (alfalfa hay = 18.07% crude protein, 2186.90 kcal kg$^{-1}$ …


Effect Of Light Sources On Productivity, Welfare Aspects, And Economic Evaluation Ofcommercial Layers, FAZAL RAZIQ, JIBRAN HUSSAIN, ATHAR MAHMUD, KHALID JAVED 2021 TÜBİTAK

Effect Of Light Sources On Productivity, Welfare Aspects, And Economic Evaluation Ofcommercial Layers, Fazal Raziq, Jibran Hussain, Athar Mahmud, Khalid Javed

Turkish Journal of Veterinary & Animal Sciences

The present study was aimed to evaluate the influence of incandescent, fluorescent, and light emitting diode (LED) lights on the productive efficiency and economic aspects of laying hens. For this, 216 laying hens were distributed into three groups of light sources with six replicates each. Each replicate consisted of 12 birds. The data were analyzed in SAS 9.1 (SAS InstituteInc., Cary, NC, USA) by applying one-way ANOVA technique, and significant means were compared through least significant difference test. In the present study, LED light significantly improved the body weight, body weight gain, percentage of egg production, FCR per dozen and …


Immunoglobulins And Acute Phase Proteins In Van Cats - Associations With Sex, Age, Andeye Colour, PINAR COŞKUN, VAHDETTİN ALTUNOK, FİLİZ KAZAK, NAZMİ YÜKSEK 2021 TÜBİTAK

Immunoglobulins And Acute Phase Proteins In Van Cats - Associations With Sex, Age, Andeye Colour, Pinar Coşkun, Vahdetti̇n Altunok, Fi̇li̇z Kazak, Nazmi̇ Yüksek

Turkish Journal of Veterinary & Animal Sciences

It was aimed to determine concentrations of plasma immunoglobulins (IgG, IgA, IgM) and acute phase proteins (?-1 acid glycoprotein, serum amyloid A, ceruloplasmin) and the association of these parameters with age, sex, and eye colour in Van cats. Blood plasma of healthy, forty-seven Van cats (Van Cat Home) fed with standard cat food were involved in the study. Cats were divided into four groups based on age ( 5) and eye colour (amber-amber, amber-blue, blue-amber, and blue-blue eyes described from left to right), and two groups based on sex (male and female). Plasma IgG, IgA, IgM, ?-1 acid glycoprotein, serum …


Effects Of Leptin And Thyroglobulin Gene Polymorphisms On Beef Quality In Holsteinbreed Bulls In Turkey, SÜLEYMAN KÖK, GÜLDAN VAPUR 2021 TÜBİTAK

Effects Of Leptin And Thyroglobulin Gene Polymorphisms On Beef Quality In Holsteinbreed Bulls In Turkey, Süleyman Kök, Güldan Vapur

Turkish Journal of Veterinary & Animal Sciences

The relationship between three single-nucleotide polymorphisms (SNP) markers and the beef quality of Turkish Holstein bulls (THB) was investigated. The markers in leptin (LEP) gene (E2JW and E2FB) and thyroglobulin (TG) gene (C422T) were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in one hundred THB. Texture, pH, and marbling score (MS) are three of the most dominating determinants of meat quality. In THB, the relationship between 3 SNPs and beef quality characteristics of musculus longissimus dorsi (MLD) was investigated. The mean pH value of the raw beef samples with the TG G422T CC genotype was 5.33 ± 0.30 …


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