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Viability Of Human Periodontal Ligament Fibroblasts After Time In Tooth Storage Media, Scott MacDonald 2017 Marquette University

Viability Of Human Periodontal Ligament Fibroblasts After Time In Tooth Storage Media, Scott Macdonald

Master's Theses (2009 -)

Periodontal ligament cell viability at the time of tooth replantation is of utmost importance for proper repair of the damaged tissues (1). Multiple different options are currently available for storage of an avulsed tooth. The goal of this study was to investigate new storage media and evaluate their ability to maintain cell viability over time. Materials and Methods: Cultured human periodontal ligament fibroblasts (hPDLF) were seeded on 96-well plates. These cells were exposed to different storage media including: Hank’s Balanced Salt Solution (HBSS), EMT Toothsaver (EMT), Save-A-Tooth (SAT), trehalose (T300), cell culture media, water, and different formulations of HBSS combined …


Prevalence And Clinical Characteristics Of Teeth Extracted With A Diagnosis Of Cracked Tooth: A Retrospective Study, Riley B. Sturgill 2017 Virginia Commonwealth University

Prevalence And Clinical Characteristics Of Teeth Extracted With A Diagnosis Of Cracked Tooth: A Retrospective Study, Riley B. Sturgill

Theses and Dissertations

The body of knowledge that exists regarding cracked teeth is limited. The purpose of this study was to determine the prevalence of cracks among extracted teeth. This retrospective longitudinal cohort study included patients of the Virginia Commonwealth University School of Dentistry that underwent extraction procedures over a 6 year period. The sample consisted of 20,408 patients and 40,870 teeth. Statistical analysis software was used to identify diagnoses of a crack, fracture, or split tooth prior to extraction of the tooth by analyzing the Electronic Health Record (EHR) (axiUm™, Version 6.03.03.1035, Exan Corporation, Vancouver, BC, Canada). There were 3,228 teeth …


Evaluation Of Volumetric Change Of Periapical Lesions After Apicoectomy As A Measure Of Postsurgical Healing Utilizing Cone Beam Computed Tomography, Eshwar Arasu 2017 Virginia Commonwealth University

Evaluation Of Volumetric Change Of Periapical Lesions After Apicoectomy As A Measure Of Postsurgical Healing Utilizing Cone Beam Computed Tomography, Eshwar Arasu

Theses and Dissertations

The aim of this study was to evaluate whether volumetric changes in persistent periapical lesions can be detected in follow-ups six months to five years after apicoectomy using cone-beam computed tomography. Patients with a previous treatment history of apicoectomy and for whom a pre-surgical CBCT scan was taken between November 2010 and December 2015 were invited to participate in the study. A post-surgical CBCT image of the treated tooth was obtained at the recall visit. Volumetric and linear measurements of periapical lesions on initial and postoperative CBCT images were performed using DiThreshGUI software and two calibrated examiners—a board-certified endodontist and …


A Comparative Study Of Intraradicular Enterococcus Faecalis Biofilm Removal With Three Root Canal Treatment Systems: A Scanning Electron Microscopy Evaluation, Cyrous Ardalan 2017 Virginia Commonwealth University

A Comparative Study Of Intraradicular Enterococcus Faecalis Biofilm Removal With Three Root Canal Treatment Systems: A Scanning Electron Microscopy Evaluation, Cyrous Ardalan

Theses and Dissertations

The objective of this study was to evaluate the biofilm removal efficacy of three root canal treatment systems: ProUltra® PiezoFlow™, traditional needle irrigation, and the GentleWave® system in an ex-vivo benchtop study. Twenty-four extracted maxillary and mandibular molars were selected. Teeth were all instrumented to a master apical file size #25 with 4% taper. Teeth were then randomly divided into four experimental groups and two control groups. The root canals were inoculated with a culture of Enterococcus faecalis and incubated for five weeks to form a biofilm. Each group was then treated with one of the different root canal treatment …


The Prevalence, Predictive Factors, And Classification Of Intrapulpal Cracks In Maxillary Molars Requiring Endodontic Treatment, husain karashi 2017 Virginia Commonwealth University

The Prevalence, Predictive Factors, And Classification Of Intrapulpal Cracks In Maxillary Molars Requiring Endodontic Treatment, Husain Karashi

Theses and Dissertations

Diagnosis and treatment of teeth with longitudinal fractures is challenging. Cracks are usually not visible radiographically; they require a thorough evaluation to aid in diagnosis. Patients may be asymptomatic, demanding the dentist rely on clinical findings to make a diagnosis. Early diagnosis of the presence and extent of a crack is essential for the successful management of a cracked tooth. There is limited information in the literature regarding the prevalence or predictive factors of cracks extending into the pulp chamber of teeth. The purpose of this study was to determine the prevalence of intrapulpal cracks in maxillary molars and to …


A Study Of The Relationship Between Job Related Stress And Temporomandibular Disorders In Accountants Working In Jakarta, Cindy Maura Saputra, Laura Susanti Himawan, Ira Tanti 2016 Undergraduate Program, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia

A Study Of The Relationship Between Job Related Stress And Temporomandibular Disorders In Accountants Working In Jakarta, Cindy Maura Saputra, Laura Susanti Himawan, Ira Tanti

Journal of Dentistry Indonesia

Stress is a known risk factor for Temporomandibular Disorders (TMD), however there are few studies of TMD in people who work in high stress jobs. Accounting is a profession known for its high levels of stress and expectation. Objective: The aim of this study is to investigate the relationship between the intensity and frequency of job related stress and the occurrence of TMD in accountants in Jakarta. Methods: A cross-sectional study, with ethics approval from the Ethical Committee of the Faculty of Dentistry, University Indonesia, was performed on 116 accountants aged 21 to 50, living in Jakarta. Subjects were asked …


Torsion And Bending Properties Of Edgeendo Files, Chad Hansen 2016 Marquette University

Torsion And Bending Properties Of Edgeendo Files, Chad Hansen

Master's Theses (2009 -)

Introduction: One important step of root canal therapy is the process of cleaning and shaping each canal. This process involves using endodontic rotary files combined with chemical irrigants to remove pulpal remnants and infected dentin from the canal while eliminating pathogenic bacteria. It is essential to maintain proper canal anatomy while cleaning and shaping. The challenge for the practitioner is to select a rotary file system that will be flexible enough to maintain canal anatomy but strong enough to prevent breakage under normal use. File flexibility allows for better maintenance of canal anatomy. A file’s resistance to torsional fatigue reduces …


Outcomes Of Primary Endodontic Therapy Provided By Endodontic Specialists Compared To Other Providers, Jacob Burry 2016 Marquette University

Outcomes Of Primary Endodontic Therapy Provided By Endodontic Specialists Compared To Other Providers, Jacob Burry

Master's Theses (2009 -)

Introduction: The objective of this study was to compare the outcomes of initial non-surgical root canal therapy (NSRCT) for different tooth types provided by both endodontists and other providers. Methods: Using an insurance company database, 487,476 initial NSRCT procedures were followed from the time of treatment to the presence of an untoward event indicated by Current Dental Terminology (CDT) codes for retreatment, apical surgery, or extraction. Population demographics were computed for provider type and tooth location. Kaplan-Meier survival estimates were calculated for 1, 5, and 10 years. Hazard ratios for provider type and tooth location were calculated using the Cox …


Viability Of Human Periodontal Ligament Fibroblasts After Storage In Save-A-Tooth, Emt Toothsaver And Hank's Balanced Salt Solution, Wonhee Lee 2016 Marquette University

Viability Of Human Periodontal Ligament Fibroblasts After Storage In Save-A-Tooth, Emt Toothsaver And Hank's Balanced Salt Solution, Wonhee Lee

Master's Theses (2009 -)

Introduction: Viability of periodontal ligament (PDL) cells is a key prognostic factor fora replanted tooth following avulsion. Dehydration results death of PDL cells, which in turn causes ankylosis upon replantation, followed by replacement resorption. Hank’s Balanced Salt Solution (HBSS) has been recommended as the standard storage medium for an avulsed tooth. Save-A-Tooth and EMT Tooth saver (Dentosafe)are commercially available tooth storage media. With the best of our knowledge no study has yet compared these media for their effective tooth storage times. The aim of this invitro study is to evaluate Save-A-Tooth, EMT Toothsaver (Dentosafe), and HBSS for their effect on …


Evaluation Of Resin Sealer Penetration Of Dentin Following Different Final Rinses For Endodontic Irrigation Using Confocal Laser Scanning Microscopy, Omar Ezzaldin Abusteit 2016 Medical University of South Carolina

Evaluation Of Resin Sealer Penetration Of Dentin Following Different Final Rinses For Endodontic Irrigation Using Confocal Laser Scanning Microscopy, Omar Ezzaldin Abusteit

MUSC Theses and Dissertations

Introduction: The aim of the study was to directly assess the depth of sealer penetration into dentinal tubules following different final rinses and indirectly evaluate precipitation of irrigating solutions. Materials and Methods: 52 extracted maxillary incisors were prepared to size 40/04 crown down with 6% sodium hypochlorite irrigating solution. Teeth were randomly divided into 4 groups (n=13) according to the final rinse. Group 1- 17% EDTA, saline and 2% Chlorhexidine solution; Group 2- SmearOFFâ„¢; Group 3- QMix®; Group 4- control with saline. Each final rinse was 3 ml over 1 minute placed with a 30-gauge needle 3 mm short from …


Treatment Of A Periodontic-Endodontic Lesion In A Patient With Aggressive Periodontitis, Mina D. Fahmy, Paul G. Luepke, Mohamed Ibrahim, Arndt Guentsch 2016 Marquette University

Treatment Of A Periodontic-Endodontic Lesion In A Patient With Aggressive Periodontitis, Mina D. Fahmy, Paul G. Luepke, Mohamed Ibrahim, Arndt Guentsch

School of Dentistry Faculty Research and Publications

Case Description: This case report describes the successful management of a left mandibular first molar with a combined periodontic-endodontic lesion in a 35-year-old Caucasian woman with aggressive periodontitis using a concerted approach including endodontic treatment, periodontal therapy, and a periodontal regenerative procedure using an enamel matrix derivate. In spite of anticipated poor prognosis, the tooth lesion healed. This case report also discusses the rationale behind different treatment interventions. Practical Implication. Periodontic-endodontic lesions can be successfully treated if dental professionals follow a concerted treatment protocol that integrates endodontic and periodontic specialties. General dentists can be the gatekeepers in managing …


Comparison Of Treatment Planning Decisions When Combining Cbct And Digital Radiography Verses Digital Radiography Alone, McKay Barlow Packer 2016 Virginia Commonwealth University

Comparison Of Treatment Planning Decisions When Combining Cbct And Digital Radiography Verses Digital Radiography Alone, Mckay Barlow Packer

Theses and Dissertations

Cone beam computed tomography (CBCT) is the recommended imaging modality of choice for evaluating previous endodontic treatment (1). The aim of this study was to compare treatment planning decisions made when evaluating previous endodontic treatment to determine if treatment planned and projected prognosis differs when digital radiography is used alone or in combination with CBCT. A retrospective chart review was conducted. Patients for whom a CBCT was taken were included in the study. Twenty-eight patients qualified. Patient’s periapical digital radiographs (Dexis©) were evaluated by 2 calibrated endodontists, a treatment plan was identified and a prognosis was projected. Later the same …


Assessment Of Changes In The Size Of Periapical Radiolucencies 3-12 Months Post Non-Surgical Root Canal Treatment Using Cbct Imaging: A Pilot Study, Jeremy W. Fike DDS 2016 Virginia Commonwealth University

Assessment Of Changes In The Size Of Periapical Radiolucencies 3-12 Months Post Non-Surgical Root Canal Treatment Using Cbct Imaging: A Pilot Study, Jeremy W. Fike Dds

Theses and Dissertations

The purpose of this study was to assess the changes in size of periapical lesions 3-12 months following root canal treatment using CBCT. Patients who had non-surgical root canal therapy (NSRCT) or non-surgical retreatment (ReTx) from July 30,2014 to August 19, 2015 with a periapical lesion of endodontic origin and received NSRCT or ReTx and had a pre-treatment or intra-treatment CBCT were invited to participate. Volumetric and linear measurements of periapical lesions on initial and post- treatment CBCT images were performed. A total of 20 patients with 23 treated teeth with 30 separate periapical radiolucent lesions returned for follow up …


Analysis Of Radiographic Changes Associated With The Periradicular Diagnosis Of Symptomatic Apical Periodontitis, Preeti Batra 2016 VCU

Analysis Of Radiographic Changes Associated With The Periradicular Diagnosis Of Symptomatic Apical Periodontitis, Preeti Batra

Theses and Dissertations

Symptomatic apical periodontitis (SAP) is indicative of inflammation of the periodontal ligament. It may or may not be accompanied by radiographic changes and may occur with vital or necrotic pulpal diagnosis. Purpose of this study was to analyze clinical and radiographic presentations of SAP in a retrospective electronic dental chart and digital radiograph review utilizing the endodontic diagnostic template note of predoctoral dental school patients presenting with SAP. The aim was to determine prevalence of periapical radiolucencies (PARLs) in SAP, association of SAP to pulpal diagnosis, and define associated radiographic changes. Most prevalent pulpal diagnosis with SAP was symptomatic irreversible …


The Incidence Of Root Dentinal Micro-Cracks Caused By Reciprocating And Continuous Rotary Instrumentation, Stephen N. Schroeder 2016 Virginia Commonwealth University

The Incidence Of Root Dentinal Micro-Cracks Caused By Reciprocating And Continuous Rotary Instrumentation, Stephen N. Schroeder

Theses and Dissertations

The aim of this study was to compare the incidence of root canal dentinal micro-cracks after canal instrumentation using reciprocating files (WaveOne Gold® and Twisted Adaptive®) and continuous rotation files (Edge Evolve® and EndoSequence®) in an ex-vivo benchtop study. This project used a novel methodology of finding dentinal defects using the “K-cube”, which allows evaluators to visualize sectioned root surfaces before instrumentation and after instrumentation. Mesial roots from 40 human mandibular first molars were divided into 4 groups of 10 for each file type. Root section pictures were taken with a Zeiss Discovery V20 stereomicroscope before and after canal instrumentation. …


Comparison Of Three Different Nickel-Titanium Endodontic Rotary Systems In Shaping Simulated S-Shaped Canals, Elineida Perez 2015 Marquette University

Comparison Of Three Different Nickel-Titanium Endodontic Rotary Systems In Shaping Simulated S-Shaped Canals, Elineida Perez

Master's Theses (2009 -)

Introduction: Preparation error may result during mechanical instrumentation because of the complex anatomy of the root canal. Thus, flexibility and resistance to fracture are ideal properties that endodontic instruments should have. The aim of the study was to compare the shaping ability of three different nickel-titanium rotary instruments in simulated S-shape root canals by measuring transportation and canal volume change CVC. Materials and Methods: A total of 60 S-shaped canals in resin blocks were randomly allocated into 3 groups (n=20): Vortex Blue (Dentsply-Tulsa Dental Specialties), HyFlex CM (Coltène/Whaledent) and EndoSequence (Brasseler USA). Canals were filled with dye and secured in …


Differential Scanning Calorimetric (Dsc) Study Of New And Sterilized Nickel-Titanium Rotary Endodontic Instruments, Michael Dyriw 2015 Marquette University

Differential Scanning Calorimetric (Dsc) Study Of New And Sterilized Nickel-Titanium Rotary Endodontic Instruments, Michael Dyriw

Master's Theses (2009 -)

Introduction: Nickel-Titanium (NiTi) files have become popular in endodontic procedures compared to traditional stainless steel hand files owing to their increased flexibility which is especially advantageous in curved canals. Due to the associated costs, endodontic instruments are frequently re-used, which requires sterilization between uses. With the application of temperature NiTi has the ability to undergo phase transformations, altering the properties of the instruments. The purpose of this study was to investigate phase transformations of four brands of NiTi rotary endodontic instruments in the as-received condition and after multiple sterilization cycles under steam sterilization, using differential scanning calorimetry. Materials and Methods: …


Lowering The Number Of False Positive Responses To Electric Pulp Tests By Qualifying Patient Response, Katherine JS Lee 2015 Virginia Commonwealth University

Lowering The Number Of False Positive Responses To Electric Pulp Tests By Qualifying Patient Response, Katherine Js Lee

Theses and Dissertations

The electric pulp test (EPT) has been shown to be a valuable tool in assessing pulp vitality. One of its drawbacks, however, is its high number of false positives. The purpose of this study was to determine if qualifying the type of sensation elicited by the test could lower the percentage of false positive responses. A retrospective chart review of an eleven-month period was conducted, examining all patients with necrotic teeth tested with the EPT. Of 189 teeth, when the patient responded to EPT at first sensation 23.3% of teeth were incorrectly identified as vital. When the patient was asked …


Restorative Characteristics Of Intrapulpally Cracked Teeth, Charles M. Beavers 2015 VCU

Restorative Characteristics Of Intrapulpally Cracked Teeth, Charles M. Beavers

Theses and Dissertations

Cracked teeth have long presented a diagnostic challenge. Previous investigators have considered possible predictive factors, many of which revolve around the tooth’s restorative characteristics. Few have investigated the restorative status of teeth with cracks extending into the pulp chamber. The purpose of this investigation was to determine the prevalence of the different types of restorations present in intrapulpally cracked teeth and determine any other restorative factors that may aid in predicting the presence or extent of an intrapulpal crack. Intrapulpally cracked molars requiring endodontic therapy at the VCU Graduate Endodontic Practice were included in this study. For each tooth, the …


Treatment Decisions Involving Teeth With Intrapulpal Cracks: A Survey Of Endodontists, Sheldon M. Sealey 2015 Virginia Commonwealth University

Treatment Decisions Involving Teeth With Intrapulpal Cracks: A Survey Of Endodontists, Sheldon M. Sealey

Theses and Dissertations

There is no universal protocol for diagnosing, treating and managing cracked teeth. The purpose of this survey was to investigate the use of traditional methods of crack detection and to explore how treatment decisions were made using an intrapulpal crack classification. The electronic survey was sent to 1115 active members of the American Association of Endodontists (AAE) and The Digital Office (TDO™) community. Comparisons were assessed using logistic or repeated-measures regression. The most often used diagnostic method was probing. When the crack involved one wall, 85% of respondents would complete root canal therapy > 50% of the time or …


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