Plant Biology Commons^™

Herbaceous 2012 (April 9), Amy L. Myers, Julia I. Chapman, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

Herbaceous vegetation data collected in 2012 at Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.

Evidence Of Reduced Arbuscular Mycorrhizal Fungal Colonization In Multiple Lines Of Bt Maize, Tanya E. Cheeke, Todd N. Rosenstiel, Mitchell B. Cruzan

Biology Faculty Publications and Presentations

Premise of the Study: Insect-resistant Bacillus thuringiensis (Bt) maize is widely cultivated, yet few studies have examined the interaction of symbiotic arbuscular mycorrhizal fungi (AMF) with different lines of Bt maize. As obligate symbionts, AMF may be sensitive to genetic changes within a plant host. Previous evaluations of the impact of Bt crops on AMF have been inconsistent, and because most studies were conducted under disparate experimental conditions, the results are difficult to compare. Methods: We evaluate AMF colonization in nine Bt maize lines, differing in number and type of engineered trait, and five corresponding near-isogenic parental (P) base hybrids …

Water And Energy Balance Response Of A Riparian Wetland To The Removal Of Phragmites Australis, Phillip Mykleby

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Vegetation and climate both play integral roles in water availability, particularly for arid to semi-arid regions. Changes in these variables can lead to extreme shortages in water for regions that rely on water for crop irrigation (i.e., the Great Plains). The objective of this study is to evaluate the impacts of vegetation on water availability in the Republican River basin in central Nebraska. Decreases in streamflow have been observed in the river basin for many years and, as a result, an invasive riparian plant species (Phragmites australis) is being removed in an effort to reduce evapotranspiration and reclaim …

Synthesis Of Organoamine-Silica Hybrids Using Cashew Nut Shell Liquid Components As Templates For The Catalysis Of A Model Henry Reaction, Sc Msigala, Jeg Mdoe

Tanzania Journal of Science

Organoamine-silica hybrids were prepared by a co-condensation of tetraethoxysilane (TEOS) and different aminoalkoxysilanes, namely, 3-aminopropyltrimethoxysilane, N-(3(trimethoxysilyl)propyl)-ethylenediamine and 3[2(2-amino)ethylamino]propyl trimethoxysilane, at different ratios using cashew nut shell liquid (CNSL) components as templates. Of the major components of CNSL tested, only cardanol was found to be efficient as a template as it yielded 66% of the hybrid. The prepared materials were characterized by diffuse reflectance Fourier Transform Infrared (FTIR), Atomic Force Microscopy and acid titration. Results indicated that indeed the organoamine groups were attached onto the silica surface, with a maximum loading of 2.9 mmol organic group per g silica. The materials …

Improving Propagation Success Of Dalbegia Melanoxylon (African Blackwood) In Tanzania (I): Characterization Of Mycorrhizal Associated With D. Melanoxylon (African Blackwood) In Tanzania, Wba Washa, Ams Nyomora, Hvm Lyaruu

Tanzania Journal of Science

Dalbergia melanoxylon is a plant with valuable wood in the world and therefore is over harvested for timber while its regeneration is very low. The propagation efforts by techniques such as tissue culture or mycorrhiza have not been investigated which instigated conduction of this study. Soil and cuttings were collected from Kilwa, Kilosa and Babati for rooting test, potting media and soil characterization. The results showed that fresh soil improved rooting characteristics while sterilized soil did not due to the presence of mycorrhiza in fresh soil compared to none in sterilized media. The overall rooting in fresh soil in non-mist …

Improving Propagation Success Of D. Melanoxylon (African Blackwood) In Tanzania (Ii): Rooting Ability Of Stem And Root Cuttings Of Dalbergia Melanoxylon (African Blackwood) In Response To Rooting Media Sterilization In Tanzania, Wba Washa, Ams Nyomora, Hvm Lyaruu

Tanzania Journal of Science

Dalbergia melanoxylon is a plant with valuable wood in the world and therefore is over harvested for timber while its regeneration is very low. The propagation efforts by techniques such as tissue culture or mycorrhiza have not been investigated which instigated conduction of this study. Soil and cuttings were collected from Kilwa, Kilosa and Babati for rooting test, potting media and soil characterization. The results showed that fresh soil improved rooting characteristics while sterilized soil did not due to the presence of mycorrhiza in fresh soil compared to none in sterilized media. The overall rooting in fresh soil in non-mist …

Herbaceous 2012 (March 25), Amy L. Myers, Julia I. Chapman, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

Herbaceous vegetation data collected in 2012 at Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.

Connections Between Sphingosine Kinase And Phospholipase D In The Abscisic Acid Signaling Pathway In Arabidopsis, Liang Guo, Girish Mishra, Jennifer E. Markham, Maoyin Li, Amanda Tawfall, Ruth Welti, Xuemin Wang

Department of Biochemistry: Faculty Publications

Background: Sphingosine kinase (SPHK) and phospholipaseD(PLD) produce different lipid mediators involved in abscisic acid (ABA) response.

Results: Ablation of SPHKs and PLDα1 attenuates ABA-induced production of LCBPs and PA. Phyto-S1P closes stomata in sphk1, sphk2, but not in pldα1, whereas PA closes stomata in all mutants.

Conclusion: SPHK acts upstream of PLDα1, whereas PLDα1 promotes SPHK.

Significance: The roles of lipid messengers in the ABA signaling pathway are clarified.

Computer Simulation And Mathematical Models Of The Noncentrosomal Plant Cortical Microtubule Cytoskeleton, Ezgi Can Eren, Natarajan Gautam, Ram Dixit

Biology Faculty Research

There is rising interest in modeling the noncentrosomal cortical microtubule cytoskeleton of plant cells, particularly its organization into ordered arrays and the mechanisms that facilitate this organization. In this review, we discuss quantitative models of this highly complex and dynamic structure both at a cellular and molecular level. We report differences in methodologies and assumptions of different models as well as their controversial results. Our review provides insights for future studies to resolve these controversies, in addition to underlining the common results between various models. We also highlight the need to compare the results from simulation and mathematical models with …

Partridge Berry: Simple Beauty Belies Complexity, W. John Hayden

Biology Faculty Publications

Superficially, plants seem so simple. Rooted in place, they do not move around. And while plant growth is a dynamic process, without time-lapse photography, growth events are so imperceptibly slow that, to us impatient humans, plants seem both immobile and static. Nevertheless, there is a lot going on inside the plant body, and this is especially true for the events of reproduction that play out inside flowers and fruits. As one of my students recently commented, “I used to think it was just a matter of pollen plus stigma and, presto-change-o, seeds happen.” That student, I hope, learned otherwise, as …

Plant Wounding And Ophiostoma Mitovirus 3a (Omv3a) Influence Infection Of Creeping Bentgrass By Sclerotinia Homoeocarpa, Angela M. Orshinsky, Michael J. Boehm, Greg J. Boland

Department of Plant Pathology: Faculty Publications

Colonization and lesion development by virulent, asymptomatic and hypovirulent isolates of Sclerotinia homoeocarpa on nonwounded (NW) and wounded (W) leaves of creeping bentgrass were characterized. Hypovirulent and asymptomatic isolates contain the fungal virus, Ophiostoma mitovirus 3a, and virulent isolates are virus-free. On NW leaves, all isolates infected leaves with appressoria along cell walls and through stomata by 48 hours post-inoculation (hpi). Inter- and intracellular hyphae formed on heavily colonized, NW leaves at 96 hpi. Wound-inoculated grass had a colonization front characterized by inter- and intracellular hyphal colonization within nonsymptomatic tissues at 8 hpi by direct infection of the wound …

Evolution Of Plant Sucrose Uptake Transporters, Anke Reinders, Alicia B. Sivitz, John M. Ward

Dartmouth Scholarship

In angiosperms, sucrose uptake transporters (SUTs) have important functions especially in vascular tissue. Here we explore the evolutionary origins of SUTs by analysis of angiosperm SUTs and homologous transporters in a vascular early land plant, Selaginella moellendorffii, and a non-vascular plant, the bryophyte Physcomitrella patens, the charophyte algae Chlorokybus atmosphyticus, several red algae and fission yeast, Schizosaccharomyces pombe. Plant SUTs cluster into three types by phylogenetic analysis. Previous studies using angiosperms had shown that types I and II are localized to the plasma membrane while type III SUTs are associated with vacuolar membrane. SUT homologs were …

Vitamin Deficiencies In Humans: Can Plant Science Help?, Teresa B. Fitzpatrick, Gilles J. C. Basset, Patrick Borel, Fernando Carrari, Dean Dellapenna, Paul D. Fraser, Hanjo Hellmann, Sonia Osorio, Christophe Rothan, Victoriano Valpuesta, Catherine Caris-Veyrat, Alisdair R. Fernie

Center for Plant Science Innovation: Faculty Publications

The term vitamin describes a small group of organic compounds that are absolutely required in the human diet. Although for the most part, dependency criteria are met in developed countries through balanced diets, this is not the case for the five billion people in developing countries who depend predominantly on a single staple crop for survival. Thus, providing a more balanced vitamin intake from high-quality food remains one of the grandest challenges for global human nutrition in the coming decade(s). Here, we describe the known importance of vitamins in human health and current knowledge on their metabolism in plants. Deficits …

The Hormonal Characterization And Mapping Of A New Jasmonate Hypersensitive Mutant (Jah2) In Arabidopsis Thaliana, Hsin-Ho Wei

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Jasmonic acid (JA) is a phytohormone that plays important roles in growth, development, and defense in plants. Although many functions and mechanisms of JA are known, some pathways are still unclear. One of the simplest approaches to understanding JA signal transduction is isolating mutants that respond abnormally to JA. From the defects of such mutants we can determine the regulatory functions of JA. jasmonate hypersensitive 2 (jah2) is a JA hypersensitive mutant. JA normally inhibits the root growth of plants and the jah2 mutant has an even shorter root compared with wild-type Col-0 (wild type) under the same …

Single-Molecule Analysis Of The Microtubule Cross-Linking Protein Map65-1 Reveals A Molecular Mechanism For Contact-Angle-Dependent Microtubule Bundling, Amanda Tulin, Sheri Mcclerklin, Yue Huang, Ram Dixit

Biology Faculty Research

Bundling of microtubules (MTs) is critical for the formation of complex MT arrays. In land plants, the interphase cortical MTs form bundles specifically following shallow-angle encounters between them. To investigate how cells select particular MT contact angles for bundling, we used an in vitro reconstitution approach consisting of dynamic MTs and the MT-cross-linking protein MAP65-1. We found that MAP65-1 binds to MTs as monomers and inherently targets antiparallel MTs for bundling. Dwell-time analysis showed that the affinity of MAP65-1 for antiparallel overlapping MTs is about three times higher than its affinity for single MTs and parallel overlapping MTs. We also …

Herbivory Affects Patterns Of Plant Reproductive Effort And Seed Production, Natalie M. West

School of Biological Sciences: Dissertations, Theses, and Student Research

Insect herbivory can have a major influence on plant reproduction, and potentially drive selection for strategies that reduce or resist herbivore effects. I used a combination of field experiments and ecological modeling to examine how modifications in the patterns and timing of reproductive investment might ameliorate the consequences of herbivore damage for plant reproduction. I performed experiments to examine how changes in reproductive effort after apical damage and reduction of insect herbivory affected seed production in two thistles native to Nebraska. I then used field data to parameterize a life history model predicting the resource allocation among buds and size …

2011 Vadose Zone Nitrate Study At Hastings, Ne (Revised), Roy F. Spalding, Martin Toavs

Department of Agronomy and Horticulture: Faculty Publications

In an effort to assist Hastings Utilities in the management of their well-head protection area (WHP A) and to protect the groundwater from the impact of nitrate loading from potential nonpoint and point sources, Hastings Utilities subcontracted with the Department of Agronomy and Horticulture at the University of Nebraska for deep vadose zone coring. Continuous 60-ft soil cores were obtained at 36 sites within the apparent WHP A (Figure 1). The sites were selected by Marty Stange on the basis of availability for sampling, management, landuse , cropping history, and location within the WHPA. Nitrogen analyses of these deep soil …

Genome-Wide Analysis Of Syntenic Gene Deletion In The Grasses, James C. Schnable, Michael Freeling, Eric Lyons

Department of Agronomy and Horticulture: Faculty Publications

The grasses, Poaceae, are one of the largest and most successful angiosperm families. Like many radiations of flowering plants, the divergence of the major grass lineages was preceded by a whole-genome duplication (WGD), although these events are not rare for flowering plants. By combining identification of syntenic gene blocks with measures of gene pair divergence and different frequencies of ancient gene loss, we have separated the two subgenomes present in modern grasses. Reciprocal loss of duplicated genes or genomic regions has been hypothesized to reproductively isolate populations and, thus, speciation. However, in contrast to previous studies in yeast and teleost …

Soybean Disease Profiles I: Foliar Diseases, Loren J. Giesler, Robert M. Harveson, Tamra A. Jackson-Ziems, Kevin A. Korus, Bo Liu, Stephen N. Wegulo

Department of Plant Pathology: Faculty Publications

Extension Circular 1093 (EC1903).

Disease photographs and descriptions of Bacterial Blight, Bacterial Pustule, Brown Spot, Cercospora Leaf Blight and Purple Seed Stain, Downy Mildew, Frogeye Leaf Spot, Soybean Rust, Powdery Mildew, and Bean Pod mottle and Soybean Mosaic.

Effects Of Foliar Fungicides And Application Timing On Field Corn In Nebraska, 2011, C. M. Schleicher, T. A. Jackson

Department of Plant Pathology: Faculty Publications

A foliar fungicide efficacy timing trial was conducted at the University of Nebraska-Lincoln South Central Agricultural Laboratory near Clay Center, NE. DeKalb corn hybrid DKC 62-54, rating of “good” (6 out of 9) for gray leaf spot (GLS) and “excellent” (2 out of 9) for common rust (CR), was planted on 2 May in 30 in. rows with a target population of 32,000 plants/A. The trial area was disked with soybean as the previous year’s crop. On 9 May before plant emergence, a herbicide program of Roundup (1 pt/A), Verdict (16 fl oz/A) and Atrazine (1 lb/A) was applied to …