Plant Biology Commons^™

Intervention Of Climate-Smart Practices In Wheat Under Rice-Wheat Cropping System In Nepal, Janma Jaya Gairhe, Mandeep Adhikari, Deepak Ghimire, Arun Khatri-Chhetri, Dinesh Panday

Department of Agronomy and Horticulture: Faculty Publications

Besides a proper agronomic management followed by Nepalese farmers, wheat (Triticum aestivum L.) production has been severely affected by changing climate. There are many interventions, including climate-smart practices, to cope with this situation and possibly enhance crop and soil productivity. Field experiments were set up in a randomized complete block design with six treatments (TRT) with four replications in three locations (LOC) during wheat-growing seasons in Nepal from 2014 to 2016. Treatments included (i) Controlled Practice (CP), (ii) Improved Low (IL), (iii) Improved High (IH), (iv) Climate Smart Agriculture Low (CSAL), (v) Climate Smart Agriculture Medium (CSAM), and (vi) …

Effect Of Foliar Micronutrients (B, Mn, Fe, Zn) On Maize Grain Yield, Micronutrient Recovery, Uptake, And Partitioning, Zachary P. Stewart, Ellen T. Paparozzi, Charles S. Wortmann, Prakash Kumar Jha, Charles A. Shapiro

Department of Agronomy and Horticulture: Faculty Publications

Timing of micronutrient demand and acquisition by maize (Zea Mays L.) is nutrient spe‐ cific and associated with key vegetative and reproductive growth stages. The objective of this study was to determine the fate of foliar‐applied B, Fe, Mn, Zn, and Fe/Zn together, evaluate the effect of foliar micronutrients applied at multiple rates and growth stages on maize grain yield, and deter‐ mine their apparent nutrient recovery efficiency (ANR). Five Randomized Complete Block Design (RCBD) experiments were conducted in 2014 and 2015 at five locations across Nebraska. Total dry matter was collected at 5–6 stages, and separated into leaves, stalk, …

Genomic Resources In Plant Breeding For Sustainable Agriculture, Mahendar Thudi, Rajeev K. Varshney, James C. Schnable

Department of Agronomy and Horticulture: Faculty Publications

Climate change during the last 40 years has had a serious impact on agriculture and threatens global food and nutritional security. From over half a million plant species, cereals and legumes are the most important for food and nutritional security. Although systematic plant breeding has a relatively short history, con- ventional breeding coupled with advances in technology and crop management strategies has increased crop yields by 56 % globally between 1965− 85, referred to as the Green Revolution. Nevertheless, increased demand for food, feed, fiber, and fuel necessitates the need to break existing yield barriers in many crop plants. In …

Development Of Mping-Based Activation Tags For Crop Insertional Mutagenesis, Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacod Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh Nguyen, Adrian Stec, Lauren A.L. Mcdaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C. Nathan Hancock

Department of Agronomy and Horticulture: Faculty Publications

Modern plant breeding increasingly relies on genomic information to guide crop im- provement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to in- duce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. …

Responses Of Soil Surface Greenhouse Gas Emissions To Nitrogen And Sulfur Fertilizer Rates To Brassica Carinata Grown As A Bio-Jet Fuel, Dwarika Bhattarai, Gandura O. Abagandura, Thandiwe Nleya, Sandeep Kumar

Department of Agronomy and Horticulture: Faculty Publications

Carinata (Brassica carinata A. Braun), a non-food oilseed crop and an alternative bio-jet fuel feedstock, has received attention for its potential as a low-input option for production in the semi-arid region of the Northern Great Plains of the United States. Research addressing the impacts of nitrogen (N) and sulfur (S) fertilizers on soils and greenhouse gas (GHG; CO2, N2O, and CH4) emissions from carinata production are limited. Thus, objective of this study was to evaluate the impact of different rates of N and S fertilizers applied to carinata on soil properties and GHG emissions. Field experiments were conducted in 2017 …

Household Smoke-Exposure Risks Associated With Cooking Fuels And Cooking Places In Tanzania: A Cross-Sectional Analysis Of Demographic And Health Survey Data, Mazbahul G. Ahamad, Fahian Tanin, Nawaraji Shrestha

Department of Agronomy and Horticulture: Faculty Publications

Household smoke-exposure risk (SER) can be defined through the assessment of cooking fuels (smoke and no smoke-producing) and cooking places (indoor and outdoor) related information, which represent different levels of household air pollution. This study aimed to explore the prevalence and geographical variations in smoke-exposure risks (SERs) associated with indoor and outdoor cooking practices and use of smoke-producing and non-smoke-producing cooking fuels in Tanzania. We further investigated the social and spatial features associated with household SERs. We defined an indicator variable, the household SER, using country-level, cross-sectional data on cooking fuels and cooking places obtained from the 2015–2016 Tanzania Demographic …

Officially Confirmed Covid-19 And Unreported Covid-19–Like Illness Death Counts: An Assessment Of Reporting Discrepancy In Bangladesh, Mazbahul G. Ahamad, Fahian Tanin, Byomkesh Talukder, Monir Ahmed

Department of Agronomy and Horticulture: Faculty Publications

Reporting discrepancies between officially confirmed COVID-19 death counts and unreported COVID- 19–like illness (CLI) death counts have been evident across the world, including Bangladesh. Publicly available data were used to explore the differences between confirmed COVID-19 death counts and deaths with possible COVID-19 symptoms between March 2, 2020 and August 22, 2020. Unreported CLI death counts totaled more than half of the confirmed COVID-19 death counts during the study period. However, the reporting authority did not consider CLI deaths, which might produce incomplete and unreliable COVID-19 data and respective mortality rates. All deaths with possible COVID-19 symptoms need to be …

Soil Erosion Modelling: A Global Review And Statistical Analysis, Pasquale Borrelli, Panos Panagos, Dinesh Panday, 64 More Co-Authors

Department of Agronomy and Horticulture: Faculty Publications

To gain a better understanding of the global application of soil erosion prediction models, we comprehensively reviewed relevant peer-reviewed research literature on soil-erosion modelling published between 1994 and 2017.We aimed to identify (i) the processes and models most frequently addressed in the literature, (ii) the regions within which models are primarily applied, (iii) the regions which remain unaddressed and why, and (iv) how frequently studies are conducted to validate/evaluate model outcomes relative to measured data. To perform this task, we combined the collective knowledge of 67 soil-erosion scientists from 25 countries. The resulting database, named ‘Global Applications of Soil Erosion …

Potential Amendments For Improving Productivity Of Low Carbon Semiarid Soil, Bijesh Maharjan, Dinesh Panday, Humberto Blanco-Canqui, Maysoon M. Mikha

Department of Agronomy and Horticulture: Faculty Publications

Applying soil amendments with high C content can potentially improve soil properties and increase crop yields. The objective of this 3-yr field study was to evaluate the effects of organic amendments on soil organic C (SOC), chemical properties, crop nutrient uptake, and crop yields in a low C sandy loam soil near Scottsbluff, NE. The field was planted to dry bean (Phaseolus vulgaris L.) in 2017, maize (Zea mays L.) in 2018, and sugar beet (Beta vulgaris L.) in 2019. Char at 22.3, 44.6, 66.9, 89.2, and 133.8 Mg ha^–1; biochar at 5.6 and 11.2 …

Loss Of Comt Activity Reduces Lateral Root Formation And Alters The Response To Water Limitation In Sorghum Brown Midrib (Bmr) 12 Mutant, Manny Saluja, Feiyu Zhu, Hongfeng Yu, Harkamal Walia, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

Lignin is a key target for modifying lignocellulosic biomass for efficient biofuel production. Brown midrib 12 (bmr12) encodes the sorghum caffeic acid O-methyltransferase (COMT) and is one of the key enzymes in monolignol biosynthesis. Loss of function mutations in COMT reduces syringyl (S) lignin subunits and improves biofuel conversion rate. Although lignin plays an important role in maintaining cell wall integrity of xylem vessels, physiological and molecular consequences due to loss of COMT on root growth and adaptation to water deficit remain unexplored.

We addressed this gap by evaluating the root morphology, anatomy and transcriptome of bmr12 mutant. The mutant …

Improving Publicly Available Corn Nitrogen Rate Recommendation Tools With Soil And Weather Measurements, Curtis J. Ransom, Newell R. Kitchen, John E. Sawyer, James J. Camberato, Paul R. Carter, Richard Ferguson, Fabián G. Fernández, David W. Franzen, Carrie A.M. Laboski, D. Brenton Myers, Emerson D. Nafziger, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Improving corn (Zea mays L.) N fertilizer rate recommendation tools is necessary for improving farmers’ profits and minimizing N pollution. Research has repeatedly shown that weather and soil factors influence available N and crop N need. Adjusting available corn N recommendation tools with soil and weather measurements could improve farmers’ ability to manage N. The aim of this research was to improve publicly available N recommendation tools with site-specific soil and weather measurements. Information from49 site-years ofNresponse trials in theU.S. Midwest was used to evaluate 21 rate recommendation tools for a single (at-planting) and split (at-planting + sidedress) N …

Adaptation And Forage Productivity Of Cool-Season Grasses In The Central Usa, Kenneth P. Vogel, Robert B. Mitchell

Department of Agronomy and Horticulture: Faculty Publications

Cool-season grass species (18) and cultivars (85) were evaluated for use in seeded grasslands in the tallgrass prairie and shortgrass steppe ecoregions of the central United States at the test locations of Ithaca and Sidney, NE, respectively. Both native and introduced grasses were evaluated in sward trails. Significant differences existed among species and cultivars for all traits evaluated except for in vitro dry matter digestibility (IVDMD) among cultivars within species at Sidney. The grasses that had the best establishment, persistence, and forage yields in the Ithaca trial were introduced wheatgrass (Thinopyrum) and bromegrass (Bromus) species. At …

Sterol Biosynthesis In Four Green Algae: A Bioinformatic Analysis Of The Ergosterol Versus Phytosterol Decision Point, Adam Voshall, Nakeirah T.M. Christie, Suzanne L. Rose, Maya Khasin, James L. Van Etten, Jennifer E. Markham, Wayne R. Riekhof, Kenneth Nickerson

School of Biological Sciences: Faculty Publications

Animals and fungi produce cholesterol and ergosterol, respectively, while plants produce the phytosterols stigmasterol, campesterol, and bsitosterol in various combinations. The recent sequencing of many algal genomes allows the detailed reconstruction of the sterol metabolic pathways. Here, we characterized sterol synthesis in two sequenced Chlorella spp., the free-living C. sorokiniana, and symbiotic C. variabilis NC64A. Chlamydomonas reinhardtii was included as an internal control and Coccomyxa subellipsoidea as a plant-like outlier. We found that ergosterol was the major sterol produced by Chlorella spp. and C. reinhardtii, while C. subellipsoidea produced the three phytosterols found in plants. In silico analysis …

Root And Crown Rot Pathogens Found On Dry Beans Grown In Mozambique, Suzana Vanessa Ismael Fernandes, G. Godoy-Lutz, Celestina Nhagupana Jochua, Carlos A. Urrea, Kent M. Eskridge, James R, Steadman, Joshua Herr

Department of Plant Pathology: Faculty Publications

Dry edible beans are a vital food source in Mozambique, East Africa—one that alleviates hunger and malnutrition and adds value to the economy. In recent years, root/crown rot (RCR) pathogens have emerged as limiting constraints in dry bean production. Not much has been characterized concerning the causal agents of RCR in Mozambique. The purpose of this study was to identify the primary pathogen(s) associated with RCR dry bean samples collected at breeder nursery sites and farmer fields in Mozambique using molecular sequencing and culture-based methods. Sequencing revealed, not surprisingly, an increased diversity of fungal/oomycete operational taxonomic units when compared to …

Lichens And Biofilms: Common Collective Growth Imparts Similar Developmental Strategies, Erin C. Carr, Steven D. Harris, Joshua R. Herr, Wayne Riekhof

Department of Plant Pathology: Faculty Publications

Lichens are traditionally defined as a symbiotic relationship between fungi and algae and/or cyanobacteria. This union forms a unique structure called the thallus, which attaches to surfaces such as rocks and tree bark. Recent reports challenge the view that lichens are comprised of one fungus and one photobiont, and instead suggest that they are a consortium of microbes. Much of lichen biology remains unknown as most of our knowledge of lichens is limited to morphological characteristics with little to no functional analysis of lichen genes. However, lichens and biofilms share many similar physiological traits which when compared may assist in …

Wildlife Forage Recovery Following Boreal Wildfire, Alexis Jorgensen, Jennifer Baltzer

Theses and Dissertations (Comprehensive)

Climate change is altering the boreal wildfire regime through increases in the extent and severity of burning and reductions in fire return intervals. These changes can alter the regeneration trajectory of canopy species and ground vegetation, with implications for wildlife habitat. There is some uncertainty about the timelines of when different animal species will use burned areas as their preferred forage taxa recover following fire, and how such recovery is mediated by environmental factors. Here, we aim to address these knowledge gaps through the following questions: 1) What are the main forage types consumed by boreal wildlife and how much …

How Wetland Plants Deal With Stress, Taylor M. Sloey

Biological Sciences Faculty Publications

We all get stressed. To deal with that stress, some of us may exercise, take a bubble bath, cry, or simply leave the stressful situation. But how can you cope with stress if you are rooted in place? Plants that live in estuaries are exposed to many types of stresses from the environment, including flooding, high salt levels, low soil oxygen, and waves. Fortunately, wetland plants have developed ways to survive within these conditions, from excreting salt, to growing faster, to even breaking down cell walls to maximize air flow. Plants can tolerate different levels of stress depending on their …

Identification And Utilization Of Genetic Determinants Of Trait Measurement Errors In Image-Based, High-Throughput Phenotyping, Yan Zhou, Aaron Kusmec, Seyed Vahid Mirnezami, Lakshmi Attigala, Srikant Srinivasan, Talukder Zaki Jubery, James Schnable, Maria G. Salas Fernandez, Baskar Ganapathysubramanian, Patrick S. Schnable

Department of Agronomy and Horticulture: Faculty Publications

The accuracy of trait measurements greatly affects the quality of genetic analyses. During automated phenotyping, trait measurement errors, i.e., differences between automatically extracted trait values and ground truth, are often treated as random effects that can be controlled by increasing population sizes and/or replication number. By contrast, there is some evidence that trait measurement errors may be partially under genetic control. Consistent with this hypothesis, we observed substantial non-random, genetic contributions to trait measurement errors for five maize (Zea mays) tassel traits collected using an image-based phenotyping platform. The phenotyping accuracy varied according to whether a tassel exhibited …

Plant Physiological Responses To Environmental Change In A Marsh-Mangrove Ecotone, Matthew Sturchio

UNF Graduate Theses and Dissertations

Globally, photosynthesis (A) and autotrophic respiration (R) are the two largest physiological processes responsible for CO₂ flux. Coastal wetland ecosystems are responsible for some of the highest rates of C sequestration. Marsh grass and mangrove habitats responsible for this service are important in supporting biodiversity and preventing shoreline erosion, yet little is known about how this vegetation will respond physiologically to effects of climate and global change. In the first chapter a warming experiment was used to determine whether a C₄ marsh grass (Spartina alterniflora) and a C₃ mangrove (Avicennia …

Elucidation Of Multi-Origin Inula Helenium L. For Antimicrobial Activity And Heavy Metal Profiling As An Exemplar For The Use And Regulation Of Plants As Antibiotics., Ciara Ruth Kenny

Theses

An impending post-antibiotic era looms, thus new sources of therapeutic chemical entities are crucial to sustain effective antimicrobial chemotherapy worldwide. Plant natural products are a source of accessible, structurally diverse compounds that provide therapeutic potential. The pharmacological applications of plants in medicine can be guided by the attestation of traditional use or the application of this knowledge to uncover new leads in the drug discovery process. The range of modified in vitro methods commonly used to investigate preclinical antimicrobial efficacy of plant-derived natural products, and the associated limitations and challenges in the provision of new antimicrobial drugs from plants is …