Non-Destructive Classification And Quality Evaluation Of Proso Millet
Cultivars Using Nir Hyperspectral Imaging With Machine Learning,
2022
University of Kentucky
Non-Destructive Classification And Quality Evaluation Of Proso Millet Cultivars Using Nir Hyperspectral Imaging With Machine Learning, Laruen E. Doyle, Julia R. Loeb, Nader Ekramirad, Dipak K. Santra, Akinbode A. Adedeji
Department of Agronomy and Horticulture: Faculty Publications
Millet is a small-seeded cereal crop with big potential and remarkable characteristics such as high drought resistance, short growing time, low water footprint, and the ability to grow in acidic soil. There is a need to develop nondestructive methods for differentiation and evaluation of the quality attributes of different of proso millet cultivars grown in the U.S. Current methods of cultivar classification are either subjective or destructive, time consuming, not allowing for the whole population to be tested, and requiring trained operators and special equipment. In this study, the feasibility of using near-infrared (NIR) hyperspectral imaging (900-1700 nm) to predict …
Genetic And Biochemical Investigation Of Seed Fatty Acid Accumulation In Arabidopsis,
2022
Huazhong Agricultural University, University of Benin
Genetic And Biochemical Investigation Of Seed Fatty Acid Accumulation In Arabidopsis, Chinedu Charles Nwafor, Delin Li, Ping Qin, Long Li, Wei Zhang, Yuanwei Zhou, Jingjing Xu, Yongtai Yin, Jianbo Cao, Limin He, Fu Xiang, Chao Liu, Liang Guo, Yongming Zhou, Edgar B. Cahoon, Chunyu Zhang
Center for Plant Science Innovation: Faculty Publications
As a vegetable oil, consisting principally of triacylglycerols, is the major storage form of photosynthetically-fixed carbon in oilseeds which are of significant agricultural and industrial value. Photosynthesis in chlorophyll-containing green seeds, along with photosynthesis in leaves and other green organs, generates ATP and reductant (NADPH and NADH) needed for seed fatty acid production. However, contribution of seed photosynthesis to fatty acid accumulation in seeds have not been well-defined. Here, we report the contribution of seed-photosynthesis to fatty acid production by probing segregating green (photosynthetically-competent) and non-green or yellow (photosynthetically-non-competent) seeds in siliques of an Arabidopsis chlorophyll synthase mutant. Using this …
Insect Pest Management With Sex Pheromone
Precursors From Engineered Oilseed Plants,
2022
Lund University
Insect Pest Management With Sex Pheromone Precursors From Engineered Oilseed Plants, Hong-Lei Wang, Bao-Jian Ding, Jian-Qing Dai, Tara J. Nazarenus, Rafael Borges, Agenor Mafra-Neto, Edgar B. Cahoon, Per Hofvander, Sten Stymne, Christer Löfstedt
Center for Plant Science Innovation: Faculty Publications
Pheromones have become an environmentally friendly alternative to conventional insecticides for pest control. Most current pheromone-based pest control products target lepidopteran pests of high-value crops, as today’s manufacturing processes cannot yet produce pheromones at low enough costs to enable their use for lower-value crops, especially commodity crops. Camelina sativa seeds genetically modified to express (Z)-11-hexadecenoic acid, a sex pheromone precursor of several moth species, provided the oil from which the precursor was isolated, purified and transformed into the final pheromone. Trap lures containing this pheromone were then assessed for their capacity to manage moth pests in the field. Plant-derived pheromone …
The Importance Of Co2 Recapture In The Co2 Concentrating Mechanism Of Chlamydomonas Reinhardtii,
2022
Louisiana State University and Agricultural and Mechanical College
The Importance Of Co2 Recapture In The Co2 Concentrating Mechanism Of Chlamydomonas Reinhardtii, Ashwani Rai
LSU Doctoral Dissertations
The aim of this thesis is to investigate the CO2 concentrating mechanism (CCM) of Chlamydomonas reinhardtii and to develop a quick method for estimating the activity of carbonic anhydrases (CAs). The first project demonstrates that there are two almost identical mitochondrial CAs in C. reinhardtii, CAH4 and CAH5, that help to maintain photosynthesis and minimize the leak of CO2 generated by respiration and photorespiration. We used an RNAi approach to reduce the expression of CAH4 and CAH5 so that their physiological functions could be studied. RNAi mutants with low expression of CAH4 and CAH5 have impaired rates …
Optimization Of Nozzle, Application Height, And Speed For Uass Pesticide Applications,
2022
University of Nebraska-Lincoln
Optimization Of Nozzle, Application Height, And Speed For Uass Pesticide Applications, Trenton Houston
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Unmanned aerial spray systems (UASS) applications have the potential to be efficient pesticide application platforms under conditions that are not accessible or fit for typical pesticide application equipment. Although this type of application is still under development in the United States, UASS pesticide applications are common in Asia, as they have replaced backpack sprayers. There is limited literature on the optimization of UASS applications and many parameters need to be investigated to identify the best combination of application variables such as flight height, flight speed, and nozzle selection. The objectives were to identify the deposition patterns of a four rotor …
Neuropilin-2 Regulates Androgen-Receptor Transcriptional Activity In Advanced Prostate Cancer,
2022
University of Nebraska Medical Center
Neuropilin-2 Regulates Androgen-Receptor Transcriptional Activity In Advanced Prostate Cancer, Samikshan Dutta, Navatha Shree Polavaram, Ridwan Islam, Sreyashi Bhattacharya, Sanika Bodas, Thomas Mayr, Sohini Roy, Sophie Alvarez, Marieta I. Toma, Anza Darehshouri, Angelika Borkowetz, Stefanie Conrad, Susanne Fuessel, Manfred Wirth, Gustavo B. Baretton, Paramita Ghosh, Kenneth J. Pienta, David L. Klinkebiel, Surinder K. Batra, Michael H. Muders, Kaustubh Datta
Department of Agronomy and Horticulture: Faculty Publications
Aberrant transcriptional activity of androgen receptor (AR) is one of the dominant mechanisms for developing of castration-resistant prostate cancer (CRPC). Analyzing AR-transcriptional complex related to CRPC is therefore important towards understanding the mechanism of therapy-resistance. While studying its mechanism, we observed that a transmembrane protein called neuropilin-2 (NRP2) plays a contributory role in forming a novel AR-transcriptional complex containing nuclear pore proteins. Using immunogold electron microscopy, high-resolution confocal microscopy, chromatin immunoprecipitation, proteomics, and other biochemical techniques, we delineated the molecular mechanism of how a specific splice variant of NRP2 becomes sumoylated upon ligand stimulation and translocates to the inner nuclear …
Differential Regulation Of Flower Transpiration During Abiotic Stress In Annual Plants,
2022
Marshall University
Differential Regulation Of Flower Transpiration During Abiotic Stress In Annual Plants, Ranjita Sinha, Sara I. Zandalinas, Yosef Fichman, Sidharth Sen, Shuai Zeng, Aurelio Gómez-Cadenas, Trupti Joshi, Felix B. Fritschi, Ron Mittler
Biomedical Sciences
Heat waves occurring during droughts can have a devastating impact on yield, especially if they happen during the flowering and seed set stages of the crop cycle. Global warming and climate change are driving an alarming increase in the frequency and intensity of combined drought and heat stress episodes, critically threatening global food security. Because high temperature is detrimental to reproductive processes, essential for plant yield, we measured the inner temperature, transpiration, sepal stomatal aperture, hormone concentrations and transcriptomic response of closed soybean flowers developing on plants subjected to a combination of drought and heat stress. Here, we report that, …
Increased Signal-To-Noise Ratios Within Experimental Field Trials By Regressing Spatially Distributed Soil Properties As Principal Components,
2022
Donald Danforth Plant Science Center
Increased Signal-To-Noise Ratios Within Experimental Field Trials By Regressing Spatially Distributed Soil Properties As Principal Components, Jeffrey C. Berry, Mingsheng Qi, Balasaheb V. Sonawane, Amy Sheflin, Asaph Cousins, Jessica Prenni, Daniel P. Schachtman, Peng Liu, Rebecca S. Bart
Department of Agronomy and Horticulture: Faculty Publications
Environmental variability poses a major challenge to any field study. Researchers attempt to mitigate this challenge through replication. Thus, the ability to detect experimental signals is deter-mined by the degree of replication and the amount of environmental variation, noise, within the experimental system. A major source of noise in field studies comes from the natural heterogeneity of soil properties which create microtreatments throughout the field. In addition, the variation within different soil properties is often nonrandomly distributed across a field. We explore this challenge through a sorghum field trial dataset with accompanying plant, microbiome, and soil property data. Diverse sorghum …
Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism,
2022
University of North Texas
Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah Mchale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang
Department of Agronomy and Horticulture: Faculty Publications
Soybean oil is one of the most consumed vegetable oils worldwide. Genetic improvement of its concentration in seeds has been historically pursued due to its direct association with its market value. Engineering attempts aiming to increase soybean seed oil presented different degrees of success that varied with the genetic design and the specific variety considered. Understanding the embryo’s responses to the genetic modifications introduced, is a critical step to successful approaches. In this work, the metabolic and transcriptional responses to AtWRI1 and AtDGAT1 expression in soybean seeds were evaluated. AtWRI1 is a master regulator of fatty acid (FA) biosynthesis, and …
Effects Of Biochar On Soil Fertility And Crop Yields: Experience From The Southern Highlands Of Tanzania,
2022
Institute of Resource Assessment, University of Dar es Salaam, P.O. Box 35091 Dar es Salaam, Tanzania
Effects Of Biochar On Soil Fertility And Crop Yields: Experience From The Southern Highlands Of Tanzania, Peter M. Rogers, Pius Z. Yanda, Noah M. Pauline, Simon Haikola, Anders Hansson, Mathias Fridahl
Tanzania Journal of Science
The world’s agricultural production is declining due to severe loss of soil fertility through natural processes or because of human activities. Biochar has been identified as a potential soil amendment to regain its fertility and increase crop productivity. This study aimed to assess the effects of biochar on soil nutrients and crop yields in the southern highlands of Tanzania. Data were collected through key informant and household interviews, and from sampling of soils in coffee farms where biochar of maize cobs origin was incorporated at the rate of 3 t ha-1. Purposive sampling approach was deployed to identify the villages …
Comparison On Vegetation Composition And Structure In Fire Prone Forest Of Ilunde And Fire Suppressed Forest Of Kitwe In Tanzania,
2022
Department of Biology, University of Dodoma, P.O. Box 338, Dodoma, Tanzania
Comparison On Vegetation Composition And Structure In Fire Prone Forest Of Ilunde And Fire Suppressed Forest Of Kitwe In Tanzania, Nyatwere D Mganga
Tanzania Journal of Science
Fire is among the factors influencing survival and development of plants in ecosystems. This study was conducted in frequently burnt and fire suppressed miombo woodlands of Ilunde and Kitwe, respectively. Tree stem density, basal area and composition of plants were determined. The methods used involved recording the numbers of tree stems, diameter at breast height and classifying vegetation to species level. In Kitwe forest, mean stem density of 582.7 ± 52.90 stems ha– was higher than 356.8 ± 38.98 stems ha– which was recorded in Ilunde forest (t = 15.98, df = 29, p < 0.05). Similarly, a higher mean tree basal area of 16.14 ± 1.32 m2 ha– was recorded in Kitwe forest, while 8.54 ± 0.94 m2 ha– being recorded in Ilunde forest (t = 3.34, df = 14, p < 0.05). Czekanowski’s similarity coefficients for trees, shrubs and saplings, and herbs and grasses were 0.46, 0.34 and 0.22, respectively. Fire adapted species were recorded in Ilunde forest. Fire prone miombo woodland had lower tree stem density, basal area and dominated by fire adapted species. Similarity coefficient between the two forests was also low. Prescribed burning is recommended in miombo woodlands for sustainable species composition and tree structure. Keywords: vegetation; composition, structure, fire prone, miombo
The Reconstruction Of The Late Pleistocene And Holocene Vegetation Dynamics In Lake Eyasi Basin, Northern Tanzania,
2022
Department of Archaeology and Heritage Studies, College of Humanities, University of Dar es Salaam, P.O. Box 35051, Dar es Salaam, Tanzania
The Reconstruction Of The Late Pleistocene And Holocene Vegetation Dynamics In Lake Eyasi Basin, Northern Tanzania, Pastory M. Bushozi, Cassian T. Mumbi, Veronica M. Muiruri, Musa S. Mwitondi
Tanzania Journal of Science
Lake Eyasi Basin of northern Tanzania plays a pivotal role in the study of human-environment interactions and in understanding human flexibility and adaptability through technological innovations over time and space. In this study, phytoliths from ancient soils and fossil pollen proxies from radiocarbon-dated sequences from Kisimangeda on the north-eastern edge of the Lake Eyasi Basin, are used to interpret trends in climatic changes recorded since the Last Glacial Maximum (LGM) to the present. We used pollen and phytolith abundances from a core that was recovered on the northern margin of saline Lake Eyasi at the depth of five metres. The …
Chemical Constituents And Antimicrobial Evaluation Of Selected Aloe Vera Branded Commercial Products In Tanzania,
2022
Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, Dar es Salaam, Tanzania.
Chemical Constituents And Antimicrobial Evaluation Of Selected Aloe Vera Branded Commercial Products In Tanzania, Stephen S Nyandoro, Bahati F Kyando, Joan Je Munissi
Tanzania Journal of Science
Chemical compositions and antimicrobial activities of twenty-two Aloe vera branded commercial products in Tanzania, a case of marketed soaps, creams, lotions, petroleum jelly, toothpastes and hair conditioner products in Dar es Salaam, were investigated. Chemical compositions were analysed using gas chromatography-mass spectrometry (GC-MS) whereas antimicrobial activities were evaluated using agar dilution method against four bacteria species, namely Staphylococcus aureus, Escherichia coli, Klebsiella pneumoneae and Salmonella typhi) and two fungal species Candida albicans and Cryptococcus neoformans. The GC-MS analysis revealed the presence of seven non-polar constituents, namely methyl palmitate, 9-octadecenoic acid methyl ester, methyl stearate, tetratetracontane, hexacosane, and pentacosane and methyl …
Effects Of Feed Moisture Content, Soybean Ratio And Barrel Temperature On Physical And Functional Properties Of Extruded Maize-Soybean Flour Blends,
2022
Department of Food Technology and Nutrition, School of Food Technology, Nutrition and Bio-engineering, Makerere University, P.O. Box 7062, Kampala, Uganda
Effects Of Feed Moisture Content, Soybean Ratio And Barrel Temperature On Physical And Functional Properties Of Extruded Maize-Soybean Flour Blends, Robert Mugabi, Stellah Byakika, Ivan Muzira Mukisa
Tanzania Journal of Science
The effects moisture content, temperature and soybean ratio on expanded product characteristics of extruded maize-soybean blends were studied. Response surface methodology was used to study the effects of extrusion conditions on extrudate properties taking barrel temperature (BT), feed moisture content (FM) and soybean flour ratio (SR) as factors. Expansion ratio (ER), water absorption index (WAI), water solubility index (WSI), hardness and colour (b* - (+) yellowness) of the extrudates were determined. BT and FM had significant effects on ER (p < 0.05). At low FM levels, ER increased with BT. It was observed that both FM and BT significantly affected WAI and WSI. WAI increased as FM increased. Increasing FM to 20% resulted in a decrease in WSI, beyond which an increase was observed. Hardness increased significantly as feed moisture content increased. High SR content significantly increased the extrudate hardness. FM had no significant effect on colour. Colour increased as the SR and BT increased. Significant regression models explained the effects of SR, FM, and BT on all response variables. All of the response variables' R2, were higher than 0.89. Keywords: Maize, Soybean, moisture content, extrusion
Identification And Larvicidal Efficacy Of Mosquito-Repelling Plants Used In Malaria Vector Control In South-East Benin,
2022
Faculté des sciences et Techniques, Université d’Abomey-calavi (UAC), Centre de Recherches Entomologiques de Cotonou (CREC), 06 BP 2604, Bénin
Identification And Larvicidal Efficacy Of Mosquito-Repelling Plants Used In Malaria Vector Control In South-East Benin, Camille O Tante, Armel Djenontin, Saïd Chitou, Wilfried Sewade, Pierre Agbani, Martin Akogbeto, Daniel C Chougourou
Tanzania Journal of Science
After identifying issues posed by some synthetic insecticides, the scientific community is now looking for other natural products with insecticidal and/or insect repellent characteristics. This study aimed to identify the plants of the Beninese flora known for their insecticidal and/or insect repellent effects and the indigenous techniques of their use and to evaluate the combined efficacy of extracts of a few on mosquito larvae for the control of malaria vectors in the South-East of Benin. A survey was conducted and the insecticidal effects of three combinations made from Azadirachta indica, Cymbopogon nardus and Hyptis suaveolens oils on Anopheles gambiae larvae …
Avian Use Of Habitat Patches Dominated By Exotic Leucaena Leucocephala Or Native Tree Species In Urban Dar Es Salaam, Tanzania,
2022
Department of Zoology and Wildlife Conservation, University of Dar es Salaam, P.O. Box 35064, Dar es Salaam, Tanzania.
Avian Use Of Habitat Patches Dominated By Exotic Leucaena Leucocephala Or Native Tree Species In Urban Dar Es Salaam, Tanzania, Chacha Werema, Charles Wilson
Tanzania Journal of Science
There has been an incursion of an exotic Leucaena leucocephala in disturbed areas and into habitat patches of native tree species in some areas in Tanzania. However, the impacts of this exotic tree on native biodiversity are not well understood. We assessed bird use of habitat patches dominated by L. leucocephala in comparison to patches of native tree species at the University of Dar es Salaam main campus, Tanzania, by comparing bird species richness, abundance and diversity using mist netting. Based on the same mist netting efforts in each habitat patch type, patches of native trees had significantly higher overall …
Increasing The Resilience Of Plant Immunity To A Warming Climate,
2022
Duke University
Increasing The Resilience Of Plant Immunity To A Warming Climate, Jong Hum Kim, Christian Castroverde, Shuai Huang, Chao Li, Richard Hilleary, Adam Seroka, Reza Sohrabi, Diana Medina-Yerena, Bethany Huot, Jie Wang, Sharon Marr, Mary Wildermuth, Tao Chen, John Macmicking, Sheng Yang He
Biology Faculty Publications
Extreme weather conditions associated with climate change affect many aspects of plant and animal life, including the response to infectious diseases. Production of salicylic acid (SA), a central plant defence hormone, is particularly vulnerable to suppression by short periods of hot weather above the normal plant growth temperature range via an unknown mechanism. Here we show that suppression of SA production in Arabidopsis thaliana at 28 °C is independent of PHYTOCHROME B (phyB) and EARLY FLOWERING 3 (ELF3), which regulate thermo-responsive plant growth and development. Instead, we found that formation of GUANYLATE BINDING PROTEIN-LIKE 3 (GBPL3) defence-activated biomolecular condensates (GDACs) …
Identification Of Putative Snp Markers Associated With
Resistance To Egyptian Loose Smut Race(S) In Spring Barley,
2022
Assiut University
Identification Of Putative Snp Markers Associated With Resistance To Egyptian Loose Smut Race(S) In Spring Barley, Kamal A.M. Abo-Elyousr, Amira M.I. Mourad, P. Stephen Baenziger, Abdelaal H.A. Shehata, Peter E. Eckstein, Aaron D. Beattie, Ahmed Sallam
Department of Agronomy and Horticulture: Faculty Publications
Loose smut (LS) disease is a serious problem that affects barley yield. Breeding of resistant cultivars and identifying new genes controlling LS has received very little attention. Therefore, it is important to understand the genetic basis of LS control in order to genetically improve LS resistance. To address this challenge, a set of 57 highly diverse barley genotypes were inoculated with Egyptian loose smut race(s) and the infected seeds/plants were evaluated in two growing seasons. Loose smut resistance (%) was scored on each genotype. High genetic variation was found among all tested genotypes indicating considerable differences in LS resistance that …
The Pho1;2a'-M1.1 Allele Of Phosphate1 Conditions
Misregulation Of The Phosphorus Starvation Response In Maize
(Zea Mays Ssp. Mays L.),
2022
Unidad de Genómica Avanzada
The Pho1;2a'-M1.1 Allele Of Phosphate1 Conditions Misregulation Of The Phosphorus Starvation Response In Maize (Zea Mays Ssp. Mays L.), Ana Laura Alonso-Nieves, M. Nancy Salazar-Vidal, J. Vladimir Torres-Rodríguez, Leonardo M. Pérez-Vázquez, Julio A. Massange-Sánchez, C. Stewart Gillmor, Ruairidh J. H. Sawers
Center for Plant Science Innovation: Faculty Publications
Plant PHO1 proteins play a central role in the translocation and sensing of inorganic phosphate. The maize (Zea mays ssp. mays) genome encodes two co-orthologs of the Arabidopsis PHO1 gene, designated ZmPho1;2a and ZmPho1;2b. Here, we report the characterization of the transposon footprint allele Zmpho1;2a'-m1.1, which we refer to hereafter as pho1;2a. The pho1;2a allele is a stable derivative formed by excision of an Activator transposable element from the ZmPho1;2a gene. The pho1;2a allele contains an 8-bp insertion at the point of transposon excision that disrupts the reading frame and is predicted to …
Divergent Evolution Of Extreme Production Of Variant Plant
Monounsaturated Fatty Acids,
2022
University of Nebraska - Lincoln
Divergent Evolution Of Extreme Production Of Variant Plant Monounsaturated Fatty Acids, Lu Gan, Kiyoul Park, Jin Chai, Evan M. Updike, Hyojin Kim, Adam Voshall, Sairam Behera, Xiao-Hong Yu, Yuanheng Cai, Chunyu Zhang, Mark A. Wilson, Jeffrey P. Mower, Etsuko Moriyama, Chi Zhang, Sireewan Kaewsuwan, Qun Liu, John Shanklin, Edgar B. Cahoon
Center for Plant Science Innovation: Faculty Publications
Metabolic extremes provide opportunities to understand enzymatic and metabolic plasticity and biotechnological tools for novel biomaterial production. We discovered that seed oils of many Thunbergia species contain up to 92% of the unusual monounsaturated petroselinic acid (18:1Δ6), one of the highest reported levels for a single fatty acid in plants. Supporting the biosynthetic origin of petroselinic acid, we identified a Δ6-stearoyl-acyl carrier protein (18:0-ACP) desaturase from Thunbergia laurifolia, closely related to a previously identified Δ6-palmitoyl-ACP desaturase that produces sapienic acid (16:1Δ6)- rich oils in Thunbergia alata seeds. Guided by a T. laurifolia desaturase crystal structure obtained in this study, …
