Other Plant Sciences Commons^™

Sub1a-Mediated Submergence Tolerance Response In Rice Involves Differential Regulation Of The Brassinosteroid Pathway, Aaron J. Schmitz, Jing J. Folsom, Yusuke Jikamaru, Pamela C. Ronald, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Submergence 1A (SUB1A), is an ethylene response factor (ERF) that confers submergence tolerance in rice (Oryza sativa) via limiting shoot elongation during the inundation period. SUB1A has been proposed to restrict shoot growth by modulating gibberellic acid (GA) signaling.

Our transcriptome analysis indicated that SUB1A differentially regulates genes associated with brassinosteroid (BR) synthesis during submergence. Consistent with the gene expression data, the SUB1A genotype had higher brassinosteroid levels after submergence compared to the intolerant genotype. Tolerance to submergence can be activated in the intolerant genotype by pretreatment with exogenous brassinolide, which results in restricted shoot elongation …

Unleashing The Potential Of The Root Hair Cell As A Single Plant Cell Type Model In Root Systems Biology, Zhenzhen Qiao, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Plant root is an organ composed of multiple cell types with different functions.This multicellular complexity limits our understanding of root biology because -omics studies performed at the level of the entire root reflect the average responses of all cells composing the organ. To overcome this difficulty and allow a more comprehensive understanding of root cell biology, an approach is needed that would focus on one single cell type in the plant root. Because of its biological functions (i.e., uptake of water and various nutrients; primary site of infection by nitrogen-fixing bacteria in legumes), the root hair cell is an attractive …

Immunodetection Of Triticum Mosaic Virus By Das- And Dac-Elisa Using Antibodies Produced Against Coat Protein Expressed In Escherichia Coli: Potential For High-Throughput Diagnostic Methods, Satyanarayana Tatineni, Gautam Sarath, Dallas Seifers, Roy C. French

Department of Agronomy and Horticulture: Faculty Publications

Triticum mosaic virus (TriMV), an economically important virus infecting wheat in the Great Plains region of the USA, is the type species of the Poacevirus genus in the family Potyviridae. Sensitive and high-throughput serology-based detection methods are crucial for the management of TriMV and germplasm screening in wheat breeding programs. In this study, TriMV coat protein (CP) was expressed in Escherichia coli, and polyclonal antibodies were generated against purified soluble native form recombinant CP (rCP) in rabbits. Specificity and sensitivity of resulting antibodies were tested in Western immuno-blot and enzyme-linked immunosorbent assays (ELISA). In direct antigen coating (DAC)-ELISA, …

Enzyme Activity In Wheat Breeding Lines Derived From Matings Of Low Polyphenol Oxidase Parents, Somrudee Nilthong, Robert A. Graybosch, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Polyphenol oxidase (PPO) in grain plays a major role in time-dependent discoloration of wheat (Triticum aestivum L.) products, especially fresh noodles. Breeding wheat cultivars with low or nil PPO activity can reduce undesirable product darkening. The low PPO line PI 117635 was crossed to two low PPO wheats, IDO580 and ‘IDO377s’, to determine whether matings between wheats with low levels of grain PPO would result in complementation, such that lines with still lower or nil PPO would be generated. Progeny in a population derived from PI 117635/ IDO580 displayed no variation in PPO activity. In the F_3:4 populations …

Soil Sorptivity Enhancement With Crop Residue Accumulation In Semiarid Dryland No-Till Agroecosystems, T.M. Shaver, G. A. Peterson, Laj R. Ahuja, D.G. Westfall

Department of Agronomy and Horticulture: Faculty Publications

Water capture and precipitation use efficiency are of great importance in dryland cropping systems because the world's dependence on food produced in dryland areas continues to increase. Growing season evapotranspiration potential greatly exceeds growing season precipitation rates in dryland areas, creating a water deficit for crops. Management practices that positively impact soil physical properties increase the potential for soils to capture water. One way to assess the ability of soils to capture water is through the measurement of sorptivity. Sorptivity is defined as the cumulative infiltration proportionality constant and is governed by surface soil physical properties such as texture, degree …

Arbuscular Mycorrhizal Fungi Differ In Their Ability To Regulate The Expression Of Phosphate Transporters In Maize (Zea Mays L.), Hui Tian, Rhae A. Drijber, Xiaolin Li, Daniel N. Miller, Brian J. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Previous studies have found that some phosphate (Pi) starvation inducible transporter genes are downregulated and arbuscular mycorrhizal (AM) inducible Pi transporter genes are upregulated in maize roots associated with the fungus Glomus intraradices. However, little is known about the functional diversity of different AM fungal species in influencing the expression of Pi transporters in maize roots. Here, we studied the expression of two Pi transporter genes ZEAma:Pht1;3 (Pi starvation inducible) and ZEAma:Pht1;6 (AM inducible) in maize root colonized by different AM fungal inoculants. Non-mycorrhizal maize, maize colonized by Glomus deserticola (CA113), Glomus intraradices (IA506), Glomus mosseae (CA201), Gigaspora gigantea …

Functional Characterization Of Cinnamyl Alcohol Dehydrogenase And Caffeic Acid O-Methyltransferase In Brachypodium Distachyon, Gina M. Trabucco, Dominick A. Matos, Scott J. Lee, Aaron J. Saathoff, Henry D. Priest, Todd C. Mockler, Gautam Sarath, Samuel P. Hazen

Department of Agronomy and Horticulture: Faculty Publications

Background: Lignin is a significant barrier in the conversion of plant biomass to bioethanol. Cinnamyl alcohol dehydrogenase (CAD) and caffeic acid O-methyltransferase (COMT) catalyze key steps in the pathway of lignin monomer biosynthesis. Brown midrib mutants in Zea mays and Sorghum bicolor with impaired CAD or COMT activity have attracted considerable agronomic interest for their altered lignin composition and improved digestibility. Here, we identified and functionally characterized candidate genes encoding CAD and COMT enzymes in the grass model species Brachypodium distachyon with the aim of improving crops for efficient biofuel production.

Results: We developed transgenic plants overexpressing artificial microRNA designed …

Complete Plastid Genomes From Ophioglossum Californicum, Psilotum Nudum, And Equisetum Hyemale Reveal An Ancestral Land Plant Genome Structure And Resolve The Position Of Equisetales Among Monilophytes, Felix Grewe, Wenhu Guo, Emily A. Gubbels, A Katie Hansen, Jeffrey P. Mower

Department of Agronomy and Horticulture: Faculty Publications

Background: Plastid genome structure and content is remarkably conserved in land plants. This widespread conservation has facilitated taxon-rich phylogenetic analyses that have resolved organismal relationships among many land plant groups. However, the relationships among major fern lineages, especially the placement of Equisetales, remain enigmatic.

Results: In order to understand the evolution of plastid genomes and to establish phylogenetic relationships among ferns, we sequenced the plastid genomes from three early diverging species: Equisetum hyemale (Equisetales), Ophioglossum californicum (Ophioglossales), and Psilotum nudum (Psilotales). A comparison of fern plastid genomes showed that some lineages have retained inverted repeat (IR) boundaries originating …

Erratum To: Identification And Characterization Of Four Missense Mutations In Brown Midrib12 (Bmr12), The Caffeic Acid O-Methyltranferase (Comt) Of Sorghum, Scott E. Sattler, Nathan A. Palmer, Ana Saballos, Ann M. Greene, Zhanguo Xin, Gautam Sarath, Wilfred Vermerris, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

The original version of this article unfortunately contained some mistakes. The name of the enzyme “Caffeic O-methyltransferase” should be read as “Caffeic acid O-Methyltranferase” throughout the paper, including the title.

On Table 2, the fifth column should have been captured as part of the sub-heading “Unpretreated”. The corrected version is shown on the next page.

Table 2 Variation in Klason lignin content and glucose yields obtained after enzymatic saccharification at 50 °C at 60 FPU/ g cellulase of native (unpretreated) stover after 4, 20, and 96 h, and of pretreated stover after 24 h

Transcriptome Analysis Of Two Buffalograss Cultivars, Michael Wachholtz, Tiffany Heng-Moss, Paul Twigg, Lisa Baird, Guoqing Lu, Keenan Amundsen

Department of Agronomy and Horticulture: Faculty Publications

Background: Buffalograss [Buchloë dactyloides (Nutt.) Engel. syn. Bouteloua dactyloides (Nutt.) Columbus] is a United States native turfgrass species that requires less irrigation, fungicides and pesticides compared to more commonly used turfgrass species. In areas where water is limited, interest in this grass species for lawns is increasing. While several buffalograss cultivars have been developed through buffalograss breeding, the timeframe for new cultivar development is long and is limited by a lack of useful genetic resources. Two high throughput next-generation sequencing techniques were used to increase the genomic resources available for buffalograss.

Results: Total RNA was extracted and …

Genetic Dissection Of Yield And Its Component Traits Using High-Density Composite Map Of Wheat Chromosome 3a: Bridging Gaps Between Qtls And Underlying Genes, Sachin Rustgi, Mustafa N. Shafqat, Neeraj Kumar, P. Stephen Baenziger, M. Liakat Ali, Ismail M. Dweikat, B. Todd Campbell, Kulvinder Singh Gill

Department of Agronomy and Horticulture: Faculty Publications

Earlier we identified wheat (Triticum aestivum L.) chromosome 3A as a major determinant of grain yield and its component traits. In the present study, a high-density genetic linkage map of 81 chromosome 3A-specific markers was developed to increase the precision of previously identified yield component QTLs, and to map QTLs for biomass-related traits. Many of the previously identified QTLs for yield and its component traits were confirmed and were localized to narrower intervals. Four novel QTLs one each for shoot biomass (Xcfa2262-Xbcd366), total biomass (wPt2740-Xcfa2076), kernels/spike (KPS) (Xwmc664-Xbarc67), and Pseudocercosporella induced lodging ( …

Indoor Application Of Attractive Toxic Sugar Bait (Atsb) In Combination With Mosquito Nets For Control Of Pyrethroid-Resistant Mosquitoes, Zachary P. Stewart, Richard M. Oxborough, Patrick K. Tungu, Matthew J. Kirby, Mark W. Rowland, Seth R. Irish

Department of Agronomy and Horticulture: Faculty Publications

Background: Attractive toxic sugar bait (ATSB) sprayed onto vegetation has been successful in controlling Anopheles mosquitoes outdoors. Indoor application of ATSB has yet to be explored. The purpose of this study was to determine whether ATSB stations positioned indoors have the potential to kill host-seeking mosquitoes and constitute a new approach to control of mosquito-borne diseases.

Methods: Insecticides were mixed with dyed sugar solution and tested as toxic baits against Anopheles arabiensis, An. Gambiae s.s. and Culex quinquefasciatus in feeding bioassay tests to identify suitable attractant-insecticide combinations. The most promising ATSB candidates were then trialed in experimental …

Nutrient Partitioning And Grain Yield Of Tanam-Rnai Wheat Under Abiotic Stress, Mary J. Guttieri, Ricardo J. Stein, Brian M. Waters

Department of Agronomy and Horticulture: Faculty Publications

Aims — Decreased expression of TaNAM genes by RNAi results in delayed senescence and decreased grain protein, iron, and zinc concentrations. Here, we determined whether NAM expression level alters onset of senescence under stress conditions, whether delayed senescence in the TaNAM-RNAi line resulted in improved tolerance to post-anthesis abiotic stress, and determined the effects of post-anthesis abiotic stress on N and mineral remobilization and partitioning to grain.

Methods — Greenhouse-grown WT and TaNAM-RNAi wheat were characterized in two studies: three levels of N fertility or water limitation during grain fill. Studies were conducted under both optimal and heat stress …

Soybean Seed Amino Acid Content Qtl Detected Using The Universal Soy Linkage Panel 1.0 With 1,536 Snps, Benjamin D. Fallen, Catherine N. Hatcher, Fred L. Allen, Dean A. Kopsell, Arnold M. Saxton, Pengyin Chen, Stella K. Kantartzi, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is the primary source of meal used in animal feed in the U.S. However, few studies have been conducted to evaluate genomic regions controlling amino acid composition is soybean. Designing soybean seed compositions that will benefit animal production is essential. The objective of this study was to identify genomic regions controlling essential and non-essential amino acid composition in soybean seed proteins. To achieve this objective, 282 F_5:9 recombinant inbred lines (RILs) developed from a cross of Essex × Williams 82 were used. Ground soybean seed samples were analyzed for amino acids and statistically …

Development And Evaluation Of Soysnp50k, A High-Density Genotyping Array For Soybean, Qijian Song, D. L. Hyten, Gaofeng Jia, Charles V. Quigley, Edward W. Fickus, Randall L. Nelson, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

The objective of this research was to identify single nucleotide polymorphisms (SNPs) and to develop an Illumina Infinium BeadChip that contained over 50,000 SNPs from soybean (Glycine max L. Merr.). A total of 498,921,777 reads 35–45 bp in length were obtained from DNA sequence analysis of reduced representation libraries from several soybean accessions which included six cultivated and two wild soybean (G. soja Sieb. et Zucc.) genotypes. These reads were mapped to the soybean whole genome sequence and 209,903 SNPs were identified. After applying several filters, a total of 146,161 of the 209,903 SNPs were determined to be …

Mapping Soybean Aphid Resistance Genes In Pi 567598b, Carmille Bales, Guorong Zhang, Menghan Liu, Clarice Mensah, Cuihua Gu, Qijian Song, D. L. Hyten, P. B. Cregan, Dechun Wang

Department of Agronomy and Horticulture: Faculty Publications

The soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America since it was first reported in 2000. Our previous study revealed that the strong aphid resistance of plant introduction (PI) 567598B was controlled by two recessive genes. The objective of this study was to locate these two genes on the soybean genetic linkage map using molecular markers. A mapping population of 282 F_4:5 lines derived from IA2070 X E06902 was evaluated for aphid resistance in a field trial in 2009 and a greenhouse trial in 2010. Two …

Dna Fingerprinting And Anastomosis Grouping Reveal Similar Genetic Diversity In Rhizoctonia Species Infecting Turfgrasses In The Transition Zone Of Usa, B. S. Amaradasa, B. J. Horvath, D. K. Lakshman, S. E. Warnke

Department of Agronomy and Horticulture: Faculty Publications

Rhizoctonia blight is a common and serious disease of many turfgrass species. The most widespread causal agent, Thanatephorus cucumeris (anamorph: R. solani), consists of several genetically different subpopulations. In addition, Waitea circinata varieties zeae, oryzae and circinata (anamorph: Rhizoctonia spp.) also can cause the disease. Accurate identification of the causal pathogen is important for effective management of the disease. It is challenging to distinguish the specific causal pathogen based on disease symptoms or macroscopic and microscopic morphology. Traditional methods such as anastomosis reactions with tester isolates are time consuming and sometimes difficult to interpret. In the present study universally …

Chloroplast Division Protein Arc3 Regulates Chloroplast Ftsz-Ring Assembly And Positioning In Arabidopsis Through Interaction With Ftsz2, Min Zhang, Aaron J. Schmitz, Deena K. Kadirjan-Kalbach, Allan D. Terbush, Katherine W. Osteryoung

Department of Agronomy and Horticulture: Faculty Publications

Chloroplast division is initiated by assembly of a mid-chloroplast FtsZ (Z) ring comprising two cytoskeletal proteins, FtsZ1 and FtsZ2. The division-site regulators ACCUMULATION AND REPLICATION OF CHLOROPLASTS3 (ARC3), MinD1, and MinE1 restrict division to the mid-plastid, but their roles are poorly understood. Using genetic analyses in Arabidopsis thaliana, we show that ARC3 mediates division-site placement by inhibiting Z-ring assembly, and MinD1 and MinE1 function through ARC3. ftsZ1 null mutants exhibited some mid-plastid FtsZ2 rings and constrictions, whereas neither constrictions nor FtsZ1 rings were observed in mutants lacking FtsZ2, suggesting FtsZ2 is the primary determinant of Z-ring assembly in vivo. arc3 …

The Plant Science Decadal Vision: Response To The Martin Commentary, David Stern, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

The plant science community is being called upon as never before to carry out research that addresses challenges in food, climate, and health, to educate a populace inundated with science misinformation, and to excite and recruit a new generation of scientists from among that populace, all while investments in basic research are stagnant or even declining in many countries. The Decadal Vision (Plant Science Research Summit, 2013) represents a response from that community, one that launches a meaningful and ongoing dialogue about how best to accomplish these goals. In that spirit, we welcome this thoughtful commentary by Cathie Martin (Martin, …

Yield Gap Analysis—Rationale, Methods And Applications—Introduction To The Special Issue, M.K. Van Ittersum, Kenneth Cassman

Department of Agronomy and Horticulture: Faculty Publications

Yield gap analysis is an increasingly popular concept. It is a powerful method to reveal and understand the biophysical opportunities to meet the projected increase in demand for agricultural products towards 2050, and to support decision making on research, policies, development and investment that is needed. This Special Issue presents the state-of-the-art about concepts, methods and applications of yield gap analysis. The methodological papers emphasize the need for agronomically sound and relevant analyses, from local to global scales. The fourteen papers provide examples of applications to different crops, climate zones and production conditions, at various spatial extents and with different …