Mapping The Low Palmitate Fap1 Mutation And Validation
Of Its Effects In Soybean Oil And Agronomic Traits
In Three Soybean Populations,
2014
North Carolina State University
Mapping The Low Palmitate Fap1 Mutation And Validation Of Its Effects In Soybean Oil And Agronomic Traits In Three Soybean Populations, Andrea J. Cardinal, Rebecca Whetten, Sanbao Wang, Jérôme Auclair, D. L. Hyten, P. B. Cregan, Eleni Bachlava, Jason Gillman, Martha Ramirez, Ralph Dewey, Greg Upchurch, Lilian Miranda, Joesph W. Burton
Department of Agronomy and Horticulture: Faculty Publications
Soybean oil with reduced palmitic acid content is desirable to reduce the health risks associated with consumption of this fatty acid. The objectives of this study were: to identify the genomic location of the reduced palmitate fap1 mutation, determine its molecular basis, estimate the amount of phenotypic variation in fatty acid composition explained by this locus, determine if there are epistatic interactions between the fap1 and fapnc loci and, determine if the fap1 mutation has pleiotropic effects on seed yield, oil and protein content in three soybean populations. This study detected two major QTL for 16:0 content located in …
Native Insect Herbivory Limits Population Growth Rate Of A
Non-Native Thistle,
2014
University of Minnesota
Native Insect Herbivory Limits Population Growth Rate Of A Non-Native Thistle, James O. Eckberg, Brigitte Tenhumberg, Svata M. Louda
Brigitte Tenhumberg Papers
The influence of native fauna on non-native plant population growth, size, and distribution is not well documented. Previous studies have shown that native insects associated with tall thistle (Cirsium altissimum) also feed on the leaves, stems, and flower heads of the Eurasian congener Cirsium vulgare, thus limiting individual plant performance. In this study, we tested the effects of insect herbivores on the population growth rate of C. vulgare. We experimentally initiated invasions by adding seeds at four unoccupied grassland sites in eastern Nebraska, USA, and recorded plant establishment, survival, and reproduction. Cumulative foliage and floral herbivory …
Evaluation Of Soilless Media Sensors For Managing Winter-Time
Greenhouse Strawberry Production Using A Capmat System,
2014
University of Nebraska-Lincoln
Evaluation Of Soilless Media Sensors For Managing Winter-Time Greenhouse Strawberry Production Using A Capmat System, George E. Meyer, Ellen T. Paparozzi, Stacy A. Adams, Diego Scacalossi Voltan
Department of Agronomy and Horticulture: Faculty Publications
It is important for a greenhouse strawberry grower to know that their capillary mat (CapMat™) fertigation system is working correctly and that plants are receiving the correct amounts of water and fertilizer. Pots with soilless mix are not expected to hold more than 70% water on a volumetric basis. Pots with less than 40% water content continuously are not supplied enough water and nutrients to the plants. Typically, pots located near the manifold distribution system get a little more water than those at the other locations, but water use will really vary according to the factors listed above as well …
Implementing Green Roofs On Movie Theaters And Shopping Centers: Business Cases In Profitable Sustainability,
2014
Pomona College
Implementing Green Roofs On Movie Theaters And Shopping Centers: Business Cases In Profitable Sustainability, Ryan J. Miller
Pomona Senior Theses
This thesis presents the business case for installing green roofs on movie theaters and shopping centers. These businesses can then derive increased profits from the environmental benefits of reduced energy use and increased stormwater retention. After presenting the basic design and benefits of a green roof, the thesis develops stand-alone business plans for a movie theater and shopping center. The author finds that green roofs are a profitable sustainability solution for the commercial enterprise.
Quantitative Trait Loci (Qtl) That Underlie Scn Resistance In
Soybean [Glycine Max (L.) Merr.] Pi438489b By ‘Hamilton’ Recombinant Inbred Line (Ril) Population,
2014
Fayetteville State University
Quantitative Trait Loci (Qtl) That Underlie Scn Resistance In Soybean [Glycine Max (L.) Merr.] Pi438489b By ‘Hamilton’ Recombinant Inbred Line (Ril) Population, Kassem My Abdelmajid, Laura Ramos, D. L. Hyten, Jason Bond, Abdelhafid Bendahmane, Prakash R. Arelli, Victor N. Njiti, Silvia Cianzio, Stella K. Kantartzi, Khalid Meksem
Department of Agronomy and Horticulture: Faculty Publications
Soybean cyst nematode caused by Heterodera glycines Ichinohe is the most devastating pest in soybean [Glycine max (L.) Merr.]. Resistance to SCN is complex, polygenic, race and cultivar specific, and it is controlled by several quantitative trait loci (QTL). Our objective was to identify and map QTL for SCN resistance to races 3 (HG Type 0) and 5 (HG Type 2.5.7) using a high density SNP-based genetic linkage map based on the PI438489B by ‘Hamilton’ (PIxH, n=50) recombinant inbred line population. The PI438489B by Hamilton map contained 648 SNPs distributed on 31 LGs with coverage of 1,524.7 cM and …
Corn Disease Update,
2014
University of Nebraska-Lincoln
Corn Disease Update, Tamra A. Jackson-Ziems, Thomas W. Dorn
Department of Plant Pathology: Faculty Publications
The growing conditions during 2013 contributed to several disease problems in corn. Cold and wet conditions early led to development of seedling diseases. The hail-damaged corn in many areas of the state during the season led to ear rot diseases that were exacerbated by cooler conditions and increased grain moisture. Diseases have been a problem throughout the season and could extend beyond harvest into storage of some corn.
Eqtl Networks Reveal Complex Genetic Architecture In The Immature Soybean Seed,
2014
University of Minnesota
Eqtl Networks Reveal Complex Genetic Architecture In The Immature Soybean Seed, Yung-Tsi Bolon, D. Hyten, James H. Orf, Carroll P. Vance, Gary J. Muehlbauer
Department of Agronomy and Horticulture: Faculty Publications
The complex network of regulatory factors and interactions involved in transcriptional regulation within the seed is not well understood. To evaluate gene expression regulation in the immature seed, we utilized a genetical genomics approach on a soybean [Glycine max (L.) Merr.] recombinant inbred line (RIL) population and produced a genome-wide expression quantitative trait loci (eQTL) dataset. The validity of the dataset was confirmed by mapping the eQTL hotspot for flavonoid biosynthesis-related genes to a region containing repeats of chalcone synthase (CHS) genes known to correspond to the soybean inhibitor locus that regulates seed color. We then identified eQTL for …
Glyphosate-Resistant Giant Ragweed (Ambrosia Trifida) Control In Glufosinate-Resistant Soybean,
2014
University of Nebraska-Lincoln
Glyphosate-Resistant Giant Ragweed (Ambrosia Trifida) Control In Glufosinate-Resistant Soybean, Simranpreet Kaur, Lowell D. Sandell, John L. Lindquist, Amit J. Jhala
Department of Agronomy and Horticulture: Faculty Publications
Glyphosate-resistant giant ragweed is one of the most competitive weeds of agronomic crops in the United States. Early emergence and rapid growth rate makes giant ragweed a competitive weed early in the season and reduces crop yields. Therefore, early spring control of giant ragweed using a preplant herbicide is critical. Glufosinate is an alternative POST herbicide for weed control in glufosinate-resistant soybean. Field experiments were conducted at David City, NE, in 2012 and 2013 to evaluate the efficacy of preplant herbicides followed by glufosinate applied alone or in tank mixes for control of glyphosate-resistant giant ragweed in glufosinate-resistant soybean. Preplant …
Shattercane X Als-Tolerant Sorghum F1 Hybrid And Shattercane Interference In Als-Tolerant Sorghum,
2014
University of Nebraska-Lincoln
Shattercane X Als-Tolerant Sorghum F1 Hybrid And Shattercane Interference In Als-Tolerant Sorghum, Rodrigo Werle, Jared J. Schmidt, John Laborde, Angela Tran, Cody F. Creech, John L. Lindquist
Department of Agronomy and Horticulture: Faculty Publications
ALS-tolerant grain sorghum cultivars are expected to be available for farmers within the next few years. Knowing that: (i) crosses between sorghum and shattercane are likely to occur resulting in crop-to-weed gene flow; (ii) ALS-susceptible shattercane X ALS-tolerant grain sorghum F1 hybrids (hybrids) were ultimately resistant to ALS-herbicides under field conditions; and (iii) hybrid fitness is equal to, or greater than, the wild parent, we conducted a greenhouse study to compare the competitive effect of shattercane and hybrid on sorghum, and whether or not herbicide application would influence the competitive ability of the hybrid …
Corn (Zea Mays) Emergence And Early Growth As Influenced By Tansymustard (Descurainia Pinnata) Residue,
2014
University of Nebraska-Lincoln
Corn (Zea Mays) Emergence And Early Growth As Influenced By Tansymustard (Descurainia Pinnata) Residue, Rodrigo Werle, Leonardo L. Perez, Lowell D. Sandell, John L. Lindquist
Department of Agronomy and Horticulture: Faculty Publications
Farmers have observed reduced early growth of corn in the presence of tansymustard [Descurainia pinnata (Walt.) Britt.], a winter annual weed of the Brassicaceae family. A greenhouse study was conducted to evaluate the effect of tansymustard on corn (Zea mays L.) emergence and early growth. Treatments included corn growing in pots with only tansymustard root residue, with only tansymustard shoot residue, and with whole tansymustard plant residue (roots plus shoots); tansymustard plants treated with glyphosate 2 days after planting (DAP) corn seeds; and a control (no tansymustard residue). Corn emergence was recorded and biomass measured after 4 weeks of …
Phytophthora Root Rot Resistance In Soybean E00003,
2014
Michigan State University
Phytophthora Root Rot Resistance In Soybean E00003, Zhongnan Zhang, Jianjun Hao, Jiazheng Yuan, Qijian Song, D. L. Hyten, P. B. Cregan, Guorong Zhang, Cuihua Gu, Ming Li, Dechun Wang
Department of Agronomy and Horticulture: Faculty Publications
Phytophthora root rot (PRR) is a devastating disease in soybean [Glycine max (L.) Merr.] production. Michigan elite soybean E00003 is resistant to Phytophthora sojae and has been used as a resistance source in breeding. Genetic control of PRR resistance in this source is unknown. To facilitate marker-assisted selection (MAS), the PRR resistance loci in E00003 and their map locations need to be determined. In this study, a genetic mapping approach was used to identify major PRR -resistant loci in E00003. The mapping population consists of 240 F4–derived lines developed by crossing E00003 with the P. sojae susceptible …
Identification And Validation Of Quantitative Trait Loci For Seed
Yield, Oil And Protein Contents In Two Recombinant Inbred Line
Populations Of Soybean,
2014
South Dakota State University
Identification And Validation Of Quantitative Trait Loci For Seed Yield, Oil And Protein Contents In Two Recombinant Inbred Line Populations Of Soybean, Xianzhi Wang, Guo‑Liang Jiang, Marci Green, Roy A. Scott, Qijian Song, D. L. Hyten, P. B. Cregan
Department of Agronomy and Horticulture: Faculty Publications
Soybean seeds contain high levels of oil and protein, and are the important sources of vegetable oil and plant protein for human consumption and livestock feed. Increased seed yield, oil and protein contents are the main objectives of soybean breeding. The objectives of this study were to identify and validate quantitative trait loci (QTLs) associated with seed yield, oil and protein contents in two recombinant inbred line populations, and to evaluate the consistency of QTLs across different environments, studies and genetic backgrounds. Both the mapping population (SD02- 4-59 × A02-381100) and validation population (SD02- 911 × SD00-1501) were phenotyped for …
Detection And Confirmation Of Quantitative Trait Loci For Soybean Seed Isoflavones,
2014
University of Tennessee
Detection And Confirmation Of Quantitative Trait Loci For Soybean Seed Isoflavones, Christopher J. Smallwood, Catherine N. Nyinyi, Dean A. Kopsell, Carl E. Sams, Dennis R. West, Pengyin Chen, Stella K. Kantartzi, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone
Department of Agronomy and Horticulture: Faculty Publications
Interest in soybean [Glycine max (L.) Merr.] isoflavones has increased in recent years owing to numerous reported health benefits. Consequently, quantitative trait loci (QTL) detection for marker-assisted breeding for isoflavones is being examined for genetic gains. This study sought to detect QTL for soybean isoflavones in a population of 274 recombinant inbred lines derived from a cross between ‘Essex’ and ‘Williams 82’ that were subdivided and tested by maturity (early, mid, and late). The field tests were conducted in three environments in 2009 (Knoxville, TN; Harrisburg, IL; and Stuttgart, AR). The population was genotyped with 480 polymorphic single nucleotide …
A Reference Genome For Common Bean And Genome-Wide Analysis Of Dual Domestications,
2014
US Department of Energy Joint Genome Institute, Walnut Creek, California
A Reference Genome For Common Bean And Genome-Wide Analysis Of Dual Domestications, Jeremy Schmutz, Phillip E. Mcclean, Sujan Mamidi, G. Albert Wu, Steven B. Cannon, Jane Grimwood, Jerry Jenkins, Shengqiang Shu, Qijian Song, Carolina Chavarro, Mirayda Torres-Torres, Valerie Geffroy, Samira Mafi Moghaddam, Dongying Gao, Brian Abernathy, Kerrie Barry, Matthew Blair, Mark A. Brick, Mansi Chovatia, Paul Gepts, David M. Goodstein, Michael Gonzales, Uffe Hellsten, D. L. Hyten, Gaofeng Jia, James D. Kelly, Dave Kudrna, Rian Lee, Manon M.S. Richard, Phillip N. Miklas, Juan M. Osorno, Josiane Rodrigues, Vincent Thareau, Carlos A. Urrea Florez, Mei Wang, Yeisoo Yu, Ming Zhang, Rod A. Wing, P. B. Cregan, Daniel S. Rokhsar, Scott A. Jackson
Department of Agronomy and Horticulture: Faculty Publications
Common bean (Phaseolus vulgaris L.) is the most important grain legume for human consumption and has a role in sustainable agriculture owing to its ability to fix atmospheric nitrogen. We assembled 473 Mb of the 587-Mb genome and genetically anchored 98% of this sequence in 11 chromosome-scale pseudomolecules. We compared the genome for the common bean against the soybean genome to find changes in soybean resulting from polyploidy. Using resequencing of 60 wild individuals and 100 landraces from the genetically differentiated Mesoamerican and Andean gene pools, we confirmed 2 independent domestications from genetic pools that diverged before human colonization. …
Identification Of New Qtls For Seed Mineral, Cysteine,
And Methionine Concentrations In Soybean
[Glycine Max (L.) Merr.],
2014
University of Nebraska-Lincoln
Identification Of New Qtls For Seed Mineral, Cysteine, And Methionine Concentrations In Soybean [Glycine Max (L.) Merr.], Raghuprakash Kastoori Ramamurthy, Joseph Jedlicka, George L. Graef, Brian M. Waters
Department of Agronomy and Horticulture: Faculty Publications
Increased concentrations of important nutrients in edible parts of plants could result in biofortified foods. Soybean [Glycine max (L.) Merr.] is a major legume crop and an important source of certain nutrients, including protein and minerals, in human and animal diets. Understanding the underlying genetic basis of seed composition is crucial to improving seed nutrient composition. In this study we used three soybean recombinant inbred line mapping populations derived from the crosses Williams 82 × DSR-173, Williams 82 × NKS19-90 and Williams 82 × Vinton 81, and constructed a joint linkage map from these populations. Forty quantitative trait loci (QTLs) …
The Interaction Of Climate Change, Land Cover,
And Political Representation In The Usa,
2014
University of Montana
The Interaction Of Climate Change, Land Cover, And Political Representation In The Usa, Brady W. Allred, Dirac L. Twidwell, Samuel D. Fuhlendorf
Department of Agronomy and Horticulture: Faculty Publications
The difficulties in tackling climate change are inherently complex and primarily centered on political and social values. This is evident in the United States where political divisions and polarizations are fundamental barriers to advancing national policies, which in turn hinder international agreements, mitigation, and adaptation. Within the United States, the vast majority of agricultural and natural resource lands are projected to incur significant climate departures and are represented by the Republican Party. The resources and economic sectors that will be directly affected by climate change are represented by national leadership that is unlikely to accept policies to prevent or adapt …
Development Of Scar Markers And Up-Pcr
Cross-Hybridization Method For Specific Detection Of Four
Major Subgroups Of Rhizoctonia From Infected Turfgrasses,
2014
University of Nebraska-Lincoln
Development Of Scar Markers And Up-Pcr Cross-Hybridization Method For Specific Detection Of Four Major Subgroups Of Rhizoctonia From Infected Turfgrasses, Bimal S. Amaradasa, Dilip Lakshman, Brandon J. Horvath, Keenan Amundsen
Department of Agronomy and Horticulture: Faculty Publications
A rapid identification assay for Waitea circinata (anamorph: Rhizoctonia spp.) varieties zeae and circinata causing patch diseases on turfgrasses was developed based on the universally primed PCR (UPPCR) products cross-blot hybridization. Tester isolates belonging to the two varieties of W. circinata were amplified with a single UP primer L21, which generated multiple DNA fragments for each variety. Probes were prepared with UP-PCR products of each tester isolate by labeling with digoxigenin. Fieldcollected W. circinata isolates and representative isolates of different R. solani anastomosis groups (AG) and AG subgroups were amplified with L21, immobilized on nylon membrane and cross hybridized with …
Porocercospora Seminalis Gen. Et Comb. Nov.,
The Causal Organism Of Buffalograss False Smut,
2014
University of Nebraska-Lincoln
Porocercospora Seminalis Gen. Et Comb. Nov., The Causal Organism Of Buffalograss False Smut, Bimal S. Amaradasa, Hugo Madrid, Johannes Z. Groenewald, Pedro W. Crous, Keenan L. Amundsen
Department of Agronomy and Horticulture: Faculty Publications
False smut caused by Cercospora seminalis is an important disease of buffalograss (Buchloe dactyloides) affecting seed production. The pathogen prevents normal caryopsis development and causes considerable yield loss and reduced seed germination. The current taxonomic placement of the false-smut causal pathogen in the genus Cercospora is incorrect based on its morphological characteristics and DNA phylogeny. In the present study the phylogenetic position of C. seminalis is clarified based on DNA sequence analysis of three loci namely the internal transcribed spacer (ITS) region, partial nuclear ribosomal large subunit (LSU) and partial sequences of the RNA polymerase II second largest …
Drought Effects On Composition And Yield For Corn Stover,
Mixed Grasses, And Miscanthus As Bioenergy Feedstocks,
2014
Idaho National Laboratory
Drought Effects On Composition And Yield For Corn Stover, Mixed Grasses, And Miscanthus As Bioenergy Feedstocks, Rachel Emerson, Amber Hoover, Allison Ray, Jeffrey Lacey, Marnie Cortez, Courtney Payne, Douglas L. Karlen, Stuart Birrell, David Laird, Robert Kallenbach, Josh Egenolf, Matthew Sousek, Thomas Voigt
Department of Agronomy and Horticulture: Faculty Publications
Drought conditions in 2012 were some of the most severe in recent history. The purpose of this study is to examine the impact of drought on quality, quantity, and theoretical ethanol yield (TEY) of three bioenergy feedstocks, corn stover, mixed grasses from Conservation Reserve Program lands, and Miscanthus × giganteus. To assess drought effects on these feedstocks, samples from 2010 (minimal to no drought) and 2012 (severe drought) were compared from multiple locations in the US. In all feedstocks, drought significantly increased extractives and reduced structural sugars and lignin; subsequently, TEYs were reduced 10–15%. Biomass yields were significantly reduced …
Arabidopsis Lipins, Pdat1 Acyltransferase, And Sdp1
Triacylglycerol Lipase Synergistically Direct Fatty Acids
Toward Β-Oxidation, Thereby Maintaining Membrane
Lipid Homeostasis,
2014
Bioscience Department, Brookhaven National Laboratory
Arabidopsis Lipins, Pdat1 Acyltransferase, And Sdp1 Triacylglycerol Lipase Synergistically Direct Fatty Acids Toward Β-Oxidation, Thereby Maintaining Membrane Lipid Homeostasis, Jilian Fan, Chengshi Yan, Rebecca Roston, John Shanklin, Changcheng Xu
Department of Agronomy and Horticulture: Faculty Publications
Triacylglycerol (TAG) metabolism is a key aspect of intracellular lipid homeostasis in yeast and mammals, but its role in vegetative tissues of plants remains poorly defined. We previously reported that PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE1 (PDAT1) is crucial for diverting fatty acids (FAs) from membrane lipid synthesis to TAG and thereby protecting against FA-induced cell death in leaves. Here, we show that overexpression of PDAT1 enhances the turnover of FAs in leaf lipids. Using the trigalactosyldiacylglycerol1-1 (tgd1-1) mutant, which displays substantially enhanced PDAT1- mediated TAG synthesis, we demonstrate that disruption of SUGAR-DEPENDENT1 (SDP1) TAG lipase or PEROXISOMAL TRANSPORTER1 (PXA1) severely decreases …
