Horticulture Commons^™

Selection Of Bread Wheat For Low Grain Cadmium Concentration At The Seedling Stage Using Hydroponics Versus Molecular Markers, Caixia Liu, Mary J. Guttieri, Brian M. Waters, Kent M. Eskridge, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

The excessive accumulation of Cd in harvested crops grown on high-Cd soils has increased public concerns for food safety. Due to the high consumption of bread wheat (Triticum aestivum L.) per capita, high concentrations of Cd in wheat grain can significantly affect human health. Breeding is a promising way to reduce grain Cd concentration. However, a lack of efficient selection methods impedes breeding for low grain Cd concentration in bread wheat. In this study, a recombinant inbred population segregating for grain Cd concentration was used to assess the efficacy of two selection methods for decreasing grain Cd concentration in …

Optimum Droplet Size Using A Pulse-Width Modulation Sprayer For Applications Of 2,4-D Choline Plus Glyphosate, Thomas R. Butts, Chase A. Samples, Lucas X. Franca, Darrin M. Dodds, Daniel B. Reynolds, Jason W. Adams, Richard K. Zollinger, Kirk A. Howatt, Bradley K. Fritz, W. Clint Hoffmann, Joe D. Luck, Greg Kruger

Department of Agronomy and Horticulture: Faculty Publications

The delivery of an optimum herbicide droplet size using pulse-width modulation (PWM) sprayers can reduce potential environmental contamination, maintain efficacy, and provide more flexible options for pesticide applicators. Field research was conducted in 2016, 2017, and 2018 across three locations (Mississippi, Nebraska, and North Dakota) for a total of 6 site-years. The objectives were to evaluate the efficacy of a range of droplet sizes (150 μm [Fine] to 900 μm [Ultra Coarse]) using a 2,4-D choline plus glyphosate pre-mixture and to create novel weed management recommendations using PWM sprayer technology. A pooled site-year generalized additive model explained less than 5% …

Context-Specific Genomic Selection Strategies Outperform Phenotypic Selection For Soybean Quantitative Traits In The Progeny Row Stage, Christopher J. Smallwood, Arnold M. Saxton, Jason D. Gillman, Hem S. Bhandari, Phillip A. Wadl, Benjamin D. Fallen, David L. Hyten, Qijian Song, Vincent R. Pantalone

Department of Agronomy and Horticulture: Faculty Publications

Evaluating different breeding selection strategies for relative utility is necessary to choose those that maximize efficiency. Soybean [Glycine max (L.) Merr.] seed yield and fatty acid, protein, and oil contents are all commercially important traits that display complex quantitative inheritance. A soybean population consisting of 860 F₅–derived recombinant inbred lines (RILs), genotyped with 4867 polymorphic single nucleotide polymorphism (SNPs) was used to compare phenotypic and context specific genomic selection (GS) strategies. To simulate progeny rows, each RIL was grown in a single plot in 2010 in Knoxville, TN, and phenotype was recorded. A subset of 276 RILs …

Cover Crops And Corn Residue Removal: Impacts On Soil Hydraulic Properties And Their Relationships With Carbon, Michael Sindelar, Humberto Blanco-Canqui, Virginia L. Jin, Richard B. Ferguson

Department of Agronomy and Horticulture: Faculty Publications

Large-scale crop residue removal may negatively affect soil water dynamics. Integrating cover crop (CC) with crop residue management can be a strategy to offset potential adverse effects of residue removal. We studied: (i) the impact of corn (Zea mays L.) residue removal (56%) with and without the use of winter rye (Secale cereale L.) CC on soil hydraulic properties, (ii) whether CC would ameliorate residue removal effects on hydraulic properties, and (iii) relationships of hydraulic properties with soil organic C (SOC) and other properties under irrigated no-till continuous corn on a silt loam in south central Nebraska after …

Spatial Imaging And Screening For Regime Shifts, Daniel R. Uden, Dirac Twidwell, Craig R. Allen, Matthew O. Jones, David E. Naugle, Jeremy D. Maestas, Brady W. Allred

Department of Agronomy and Horticulture: Faculty Publications

Screening is a strategy for detecting undesirable change prior to manifestation of symptoms or adverse effects. Although the well-recognized utility of screening makes it commonplace in medicine, it has yet to be implemented in ecosystem management. Ecosystem management is in an era of diagnosis and treatment of undesirable change, and as a result, remains more reactive than proactive and unable to effectively deal with today’s plethora of non-stationary conditions. In this paper, we introduce spatial imaging-based screening to ecology. We link advancements in spatial resilience theory, data, and technological and computational capabilities and power to detect regime shifts (i.e., vegetation …

Estimating Percentages Of Fusarium-Damaged Kernels In Hard Wheat By Near-Infrared Hyperspectral Imaging, Stephen R. Delwiche, I. Torres Rodriguez, S. R. Rausch, R. A. Graybosch

Department of Agronomy and Horticulture: Faculty Publications

Fusarium head blight (FHB) is among the most common fungal diseases affecting wheat, resulting in decreased yield, low-density kernels, and production of the mycotoxin deoxynivalenol, a compound toxic to humans and livestock. Human visual analysis of representative wheat samples has been the traditional method for FHB assessment in both official inspection and plant breeding operations. While not requiring specialized equipment, visual analysis is dependent on a trained and consistent workforce, such that in the absence of these aspects, biases may arise among inspectors and evaluation dates. This research was intended to avoid such pitfalls by using longer wavelength radiation than …

Soil Fauna Accelerate Dung Pat Decomposition And Nutrient Cycling Into Grassland Soil, Kenneth S. Evans, Martha Mamo, Ana Wingeyer, Walter Schacht, Kent Eskridge, Jeff Bradshaw, Daniel Ginting

Department of Agronomy and Horticulture: Faculty Publications

Soil fauna play critical roles in various ecosystem functions and services, but empirical data measuring their impact on dung pat decomposition and subsequent nutrient cycling into rangeland soils are limited. The objective of this study was to quantify the effect of soil fauna, using dung beetle as an indicator, on dung decomposition and subsequent translocation of dung nutrients into grassland soil over time. A field experiment was conducted early in the summer season and late in the summer season of 2014 and 2015. In each season, dung beetle abundance, changes in dung properties, and subsequent translocation of dung nutrients into …

Utilizing Trait Networks And Structural Equation Models As Tools To Interpret Multi‑Trait Genome‑Wide Association Studies, Mehdi Momen, Malachy T. Campbell, Harkamal Walia, Gota Morota

Department of Agronomy and Horticulture: Faculty Publications

Background: Plant breeders seek to develop cultivars with maximal agronomic value, which is often assessed using numerous, often genetically correlated traits. As intervention on one trait will affect the value of another, breeding decisions should consider the relationships among traits in the context of putative causal structures (i.e., trait networks). While multi-trait genome-wide association studies (MTM-GWAS) can infer putative genetic signals at the multivariate scale, standard MTM-GWAS does not accommodate the network structure of phenotypes, and therefore does not address how the traits are interrelated. We extended the scope of MTM-GWAS by incorporating trait network structures into GWAS using structural …

The Mitochondrial Genome Of Eleusine Indica And Characterization Of Gene Content Within Poaceae, Nathan D. Hall, Hui Zhang, Jeffrey P. Mower, Joseph Scott Mcelroy, Leslie R. Goertzen

Department of Agronomy and Horticulture: Faculty Publications

Plant mitochondrial (mt) genome assembly provides baseline data on size, structure, and gene content, but resolving the sequence of these large and complex organelle genomes remains challenging due to fragmentation, frequent recombination, and transfers of DNA from neighboring plastids. Themt genome for Eleusine indica (Poaceae: goosegrass) is comprehensibly analyzed here, providing key reference data for an economically significant invasive species that is also the maternal parent of the allotetraploid crop Finger millet (Eleusine coracana). The assembled E. indica genome contains 33 protein coding genes, 6 rRNA subunits, 24 tRNA, 8 large repetitive regions 15 kb of transposable elements across a …

A Spatial Framework For Ex-Ante Impact Assessment Of Agricultural Technologies, José F. Andrade, Juan I. Rattalino Edreira, Andrew Farrow, Marloes P. Van Loon, Peter Q. Craufurd, Jairos Rurinda, Shamie Zingore, Jordan Chamberlin, Lieven Claessens, Julius Adewopo, Martin K. Van Ittersum, Kenneth G. Cassman, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Traditional agricultural research and extension relies on replicated field experiments, on-farm trials, and demonstration plots to evaluate and adapt agronomic technologies that aim to increase productivity, reduce risk, and protect the environment for a given biophysical and socio-economic context. To date, these efforts lack a generic and robust spatial framework for ex-ante assessment that: (i) provides strategic insight to guide decisions about the number and location of testing sites, (ii) define the target domain for scaling-out a given technology or technology package, and (iii) estimate potential impact from widespread adoption of the technology(ies) being evaluated. In this study, we developed …

Adaptation Of Photosynthesis To Water Deficit In The Reproductive Phase Of A Maize (Zea Mays L.) Inbred Line, H.F. Zheng, L.F. Xin, J.M. Guo, J. Mao, X.P. Han, L. Jia, B.Y. Zheng, C.G. Du, Roger W. Elmore, Q.H. Yang, R.X. Shao

Department of Agronomy and Horticulture: Faculty Publications

Photosynthesis is sensitive to water deficit (WD) stress. Maize (Zea mays L.) yield is vulnerable to water stress, especially if it occurs during the reproductive stage. In this study, the expression patterns of photosynthesis-related genes, together with photosynthetic gas-exchange and fluorescence parameters were investigated in a maize inbred line exposed to 50% of field water capacity (moderate WD) for 15 d after tassel emergence. The results demonstrated that WD down-regulated expression of psbA, psbB, psbC, psbP, psaA, psaB, and cab, especially at later periods of WD stress. Besides, with the increased WD stress, the steady decline in the value of …

Engineering Linear, Branched-Chain Triterpene Metabolism In Monocots, Chase Kempinski, Zuodong Jiang, Garrett Zinck, Shirley J. Sato, Zhengxiang Ge, Thomas E. Clemente, Joseph Chappell

Department of Agronomy and Horticulture: Faculty Publications

Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to yield farnesyl diphosphate (FPP), catalyzed by FPP synthase (FPS). Squalene synthase (SQS) condenses two molecules of FPP to generate the symmetrical product squalene, the first committed precursor to sterols and most other triterpenes. In the green algae Botryococcus braunii, two FPP molecules can also be condensed in an asymmetric manner yielding the more highly branched triterpene, botryococcene. Botryococcene …

Relationships Between Wildfire Burn Severity, Cavity-Nesting Bird Assemblages, And Habitat In An Eastern Ponderosa Pine Forest, Emma C. Keele, Victoria M. Donovan, Caleb P. Roberts, Sarah M. Nodskov, Carissa L. Wonkka, Craig R. Allen, Larkin A. Powell, David A. Wedin, David G. Angeler, Dirac Twidwell

Department of Agronomy and Horticulture: Faculty Publications

Historically, eastern ponderosa pine (Pinus ponderosa) forests were described as sparse patches of old-growth trees maintained by frequent, low-severity fires; however, in recent decades, there have been a number of large mixed-severity wildfires throughout the range of these forests. Wildlife responses to severe fire disturbance in eastern ponderosa pine forests are not well understood. Our study investigates how cavity-nesting bird species in an eastern ponderosa pine forest are impacted by burn severity. The objectives of our study were to: (1) identify the community composition of cavity-nesting birds in a 27 y old burn of mixed severity, (2) assess …

Cover Crop Mixture Diversity, Biomass Productivity, Weed Suppression, And Stability, A. M. Florence, Leon G. Higley, R. A. Drijber, Charles A. Francis, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

The diversity-productivity, diversity-invasibility, and diversity-stability hypotheses propose that increasing species diversity should lead, respectively, to increased average biomass productivity, invasion resistance, and stability. We tested these three hypotheses in the context of cover crop mixtures, evaluating the effects of increasing cover crop mixture diversity on above ground biomass, weed suppression, and biomass stability. Twenty to forty cover crop treatments were replicated three or four times at eleven sites using eighteen species representing three cover crop species each from six pre-defined functional groups: cool-season grasses, cool-season legumes, cool-season brassicas, warm-season grasses, warm-season legumes, and warm-season broadleaves. Each species was seeded as …

Winter Wheat Grain Yield Response To Fungicide Application Is Influenced By Cultivar And Rainfall, Emmanuel Byamukama, Shaukat Ali, Jonathan Kleinjan, Dalitso N. Yabwalo, Christopher Graham, Melanie Caffe-Treml, Nathan D. Mueller, John Rickertsen, William A. Berzonsky

Department of Agronomy and Horticulture: Faculty Publications

Winter wheat is susceptible to several fungal pathogens throughout the growing season and foliar fungicide application is one of the strategies used in the management of fungal diseases in winter wheat. However, for fungicides to be profitable, weather conditions conducive to fungal disease development should be present. To determine if winter wheat yield response to fungicide application at the flowering growth stage (Feekes 10.5.1) was related to the growing season precipitation, grain yield from fungicide treated plots was compared to non-treated plots for 19 to 30 hard red winter wheat cultivars planted at 8 site years from 2011 through 2015. …

Flue Gas Desulfurization Gypsum As Soil Amendment, Dinesh Panday, Richard Ferguson, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Flue gas desulfurization (FGD) gypsum is one of the by-products of a coal-fired power generation plant. Coal is the world’s most abundant and widely distributed fossil fuel. After natural gas, coal is the second primary source of energy to generate electricity globally (more than 25%) and remains a key component of the fuel mix for power generation to meet electricity demand in most of the developing countries. The U.S., China and India are the top coal producers and consumers (for production of electricity from coal sources) in the world (OECD/IEA, 2014; IEA, 2016). However, in the U.S., its contribution to …

A Key Variant In The Cis-Regulatory Element Of Flowering Gene Ghd8 Associated With Cold Tolerance In Rice, Peng Wang, Yin Xiong, Rong Gong, Ying Yang, Kai Fan, Sibin Yu

Department of Agronomy and Horticulture: Faculty Publications

Variations in the gene promoter play critical roles in the evolution of important adaptive traits in crops, but direct links of the regulatory mutation to the adaptive change are not well understood. Here, we examine the nucleotide variations in the promoter region of a transcription factor (Ghd8) that control grain number, plant height and heading date in rice. We find that a dominant promoter type of subspecies japonica displayed a high activity for Ghd8 expression in comparison with the one in indica. Transgenic analyses revealed that higher expression levels of Ghd8 delayed heading date and enhanced cold …

A Tmeff2-Regulated Cell Cycle Derived Gene Signature Is Prognostic Of Recurrence Risk In Prostate Cancer, Constantin Georgescu, Joshua M. Corbin, Sandra Thibivilliers, Zachary D. Webb, Yan D. Zhao, Jan Koster, Kar-Ming Fung, Adam S. Asch, Jonathan D. Wren, Maria J. Ruiz-Echevarria

Department of Agronomy and Horticulture: Faculty Publications

Background: The clinical behavior of prostate cancer (PCa) is variable, and while the majority of cases remain indolent, 10% of patients progress to deadly forms of the disease. Current clinical predictors used at the time of diagnosis have limitations to accurately establish progression risk. Here we describe the development of a tumor suppressor regulated, cell-cycle gene expression based prognostic signature for PCa, and validate its independent contribution to risk stratification in several radical prostatectomy (RP) patient cohorts.

Methods: We used RNA interference experiments in PCa cell lines to identify a gene expression based gene signature associated with Tmeff2, an …

Impact Of Hurricane Katrina On The Coastal Systems Of Southern Louisiana, Wen-Ching Chuang, Tarsha Eason, Ahjond Garmestani, Caleb Roberts

Department of Agronomy and Horticulture: Faculty Publications

Natural disasters, such as hurricanes and forest fires, could trigger collapse and reorganization of social-ecological systems. In the face of external perturbations, a resilient system would have capacity to absorb impacts, adapt to change, learn, and if needed, reorganize within the same regime. Within this context, we asked how human and natural systems in Louisiana responded to Hurricane Katrina, and how the natural disaster altered the status of these systems. This paper discusses community resilience to natural hazards and addresses the limitations for assessing disaster resilience. Furthermore, we assessed social and environmental change in New Orleans and southern Louisiana through …

Genome-Wide Association Study For Multiple Biotic Stress Resistance In Synthetic Hexaploid Wheat, Madhav Bhatta, Alexey Morgounov, Vikas Belamkar, Stephen N. Wegulo, Abdelfattah A. Dababat, Gul Erginbas-Orakci, Mustapha El Bouhssini, Pravin Gautam, Jesse Poland, Nilufer Akci, Lutfu Demir, Ruth Wanyera, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Genetic resistance against biotic stress is a major goal in many wheat breeding programs. However, modern wheat cultivars have a limited genetic variation for disease and pest resistance and there is always a possibility of the evolution of new diseases and pests to overcome previously identified resistance genes. A total of 125 synthetic hexaploid wheats (SHWs; 2n = 6x = 42, AABBDD, Triticum aestivum L.) were characterized for resistance to fungal pathogens that cause wheat rusts (leaf; Puccinia triticina, stem; P. graminis f.sp. tritici, and stripe; P. striiformis f.sp. tritici) and crown rot (Fusarium spp.); …