Horticulture Commons^™

Production Of Premium Waxflowers, Kevin Seaton, Nikki Poulish

Bulletins 4000 -

The cut flower trade is a highly competitive global market. Waxflowers are grown around the world—often in countries with lower cost structures or closer proximity to our main markets. Western Australian producers can only compete if their product stands out from the crowd in terms of quality, presentation and uniqueness, however, it still has to represent good value for money.

Safety Evaluations Of The Cry1ia1 Protein Found In The Transgenic Potato 'Spuntag2', Hector Quemada, Kelly Zarka, Walter Pett, Gurling Bothma

University Faculty Publications and Creative Works

The transgenic potato 'SpuntaG2' (Solanum tuberosum), which is resistant to potato tuber moth (Phthorimaea operculella), was subjected to protein safety evaluations including protein equivalency tests for the Cry1Ia1 protein from 'SpuntaG2' and bacterially produced Cry1Ia1, toxicity and allergenicity evaluations of Cry1Ia1 protein, and compositional equivalency of 'SpuntaG2' compared with non-transgenic 'Spunta'. Western blot analysis and biological activity assays showed molecular and functional equivalency between 'SpuntaG2'-derived Cry1Ia1 protein and bacteria-derived Cry1Ia1 protein. Comparison of the Cry1Ia1 amino acid sequence to known amino acid sequences revealed no significant homology to known toxins or known allergens. Acute toxicity studies using rodents were used …

An Integrative Approach To Genomic Introgression Mapping, Andrew J. Severin, Gregory A. Peiffer, Wayne W. Xu, D. L. Hyten, Bruna Bucciarelli, Jamie A. O'Rourke, Yung-Tsi Bolon, David Grant, Andrew Farmer, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker, Robert M. Stupar

Department of Agronomy and Horticulture: Faculty Publications

Near-isogenic lines (NILs) are valuable genetic resources for many crop species, including soybean (Glycine max). The development of new molecular platforms promises to accelerate the mapping of genetic introgressions in these materials. Here, we compare some existing and emerging methodologies for genetic introgression mapping: single-feature polymorphism analysis, Illumina GoldenGate single nucleotide polymorphism (SNP) genotyping, and de novo SNP discovery via RNA-Seq analysis of next-generation sequence data. We used these methods to map the introgressed regions in an iron-inefficient soybean NIL and found that the three mapping approaches are complementary when utilized in combination. The comparative RNA-Seq approach offers …

Soydb: A Knowledge Database Of Soybean Transcription Factors, Zheng Wang, Marc Libault, Trupti Joshi, Babu Valliyodan, Henry T. Nguyen, Dong Xu, Gary Stacey, Jianlin Cheng

Department of Agronomy and Horticulture: Faculty Publications

Background: Transcription factors play the crucial rule of regulating gene expression and influence almost all biological processes. Systematically identifying and annotating transcription factors can greatly aid further understanding their functions and mechanisms. In this article, we present SoyDB, a user friendly database containing comprehensive knowledge of soybean transcription factors.

Description: The soybean genome was recently sequenced by the Department of Energy-Joint Genome Institute (DOE-JGI) and is publicly available. Mining of this sequence identified 5,671 soybean genes as putative transcription factors. These genes were comprehensively annotated as an aid to the soybean research community. We developed SoyDB - a knowledge database …

Transgenic Maize Lines With Cell-Type Specific Expression Of Fluorescent Proteins In Plastids, Amir Sattarzadeh, Jonathan Fuller, Salvador Moguel, Katia Wostrikoff, Shirley Sato, Sarah Covshoff, Tom Clemente, Maureen Hanson, David Stern

Department of Agronomy and Horticulture: Faculty Publications

Plastid number and morphology vary dramatically between cell types and at different developmental stages. Furthermore, in C4 plants such as maize, chloroplast ultrastructure and biochemical functions are specialized in mesophyll and bundle sheath cells, which differentiate acropetally from the proplastid form in the leaf base. To develop visible markers for maize plastids, we have created a series of stable transgenics expressing fluorescent proteins fused to either the maize ubiquitin promoter, the mesophyll-specific phosphoenolpyruvate carboxylase (PepC) promoter, or the bundle sheath-specific Rubisco small subunit 1 (RbcS) promoter. Multiple independent events were examined and revealed that maize codon-optimized versions of YFP and …

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 11 2010, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Letter From The Dean, Michael Vayda

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

La Producción De Las Fresas Utilizando El Túnel Alto, Daniel Rowley, Brent Black, Dan Drost

All Current Publications

Las fresas locales y frescas son siempre un favorito en los mercados de productores y en los puestos ambulantes. Las fresas crecen mejor en las temperaturas alrededor de 70 a 75˚ F (21 a 24˚ C). Los inviernos fríos y los veranos calientes solo dejan una temporada de fresas muy corta. Se diseñan los invernaderos para mantener temperaturas óptimas para el crecimiento y se las puede usar para cultivar las fresas durante todo el año. Sin embargo, los invernaderos son muy costosos para construir y operar. Los túneles altos son relativamente baratos para construir, con algunos diseños costando menos de …

Presupuesto De Las Frambuesas Del Otoño En El Túnel Alto 2010, Utah State University

All Current Publications

No abstract provided.

Copy Number Variation Shapes Genome Diversity In Arabidopsis Over Immediate Family Generational Scales, Seth Debolt

Horticulture Faculty Publications

Arabidopsis thaliana is the model plant and is grown worldwide by plant biologists seeking to dissect the molecular underpinning of plant growth and development. Gene copy number variation (CNV) is a common form of genome natural diversity that is currently poorly studied in plants and may have broad implications for model organism research, evolutionary biology, and crop science. Herein, comparative genomic hybridization (CGH) was used to identify and interrogate regions of gene CNV across the A. thaliana genome. A common temperature condition used for growth of A. thaliana in our laboratory and many around the globe is 22 degrees C. …

Comparison Between In Vitro And In Vivo Antibacterial Activity Of Curcuma Zedoaria From Malaysia., Behrooz Banisalam

Student Works (2010-2019)

The rhizomes of the Zingiberaceae family are vegetables widely used in many Asian countries and their medicinal functions have been broadly discussed and accepted in many traditional recipes. In this study, the antimicrobial activity of extracts of Curcuma zedoaria from Malaysia was compared between two systems as in vitro and in vivo. The comparison was performed against four bacterial strains including two gram negative strains (E.coli and Pseudomonas aeruginosa) and two gram positive strains (Bacillus cereus and Staphylococcus aureus) using the agar well diffusion method. Extracts were made in three different solvents; petroleum ether, chloroform and methanol extracts exhibited antibacterial …

Development Of Protocols For Tissue Culture And Mutagenesis In Two Variegated Sansevieria Sp., Irmawati Akma Abdul Hapiz

Student Works (2010-2019)

Studies were conducted to develop the tissue culture protocol and mutagenesis through Gamma ray irradiation for two variegated Sansevieria species known as Sansevieria trifasciata var. Laurentii (goldband Sansevieria) and Sansevieria trifasciata var. Hahnii (birdnest Sansevieria). Seven combinations of BAP and NAA were chosen which are represented by letter A= 1.00 mg/I BAP, B = 1.00 mg/I BAP + 0.25 mg/I NAA, C = 2.00 mg/I BAP + 0.50 mg/I NAA, D = 2.00 mg/I BAP + 1.00 mg/I NAA, E = 3.00 mg/I BAP + 1.00 mg/I NAA, F = 4.00 mg/I BAP + 1.00 mg/I NAA and G = …

Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris, Jer-Young Lin, Robert M. Stupar, Christian Hans, D. L. Hyten, Scott A. Jackson

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max) has undergone at least two rounds of polyploidization, resulting in a paleopolyploid genome that is a mosaic of homoeologous regions. To determine the structural and functional impact of these duplications, we sequenced two ~1-Mb homoeologous regions of soybean, Gm8 and Gm15, derived from the most recent ~13 million year duplication event and the orthologous region from common bean (Phaseolus vulgaris), Pv5. We observed inversions leading to major structural variation and a bias between the two chromosome segments as Gm15 experienced more gene movement (gene retention rate of 81% in Gm15 versus 91% in …

Pumpkin Variety Performance With And Without Treatment For Powdery Mildew In Northern Indiana, 2009, Elizabeth T. Maynard

Midwest Vegetable Trial Reports

Pumpkins for decorative use are grown on more than 4,000 acres in Indiana. Combined acreage in Indiana, Michigan, Illinois, and Ohio represents about a quarter of pumpkins grown for decorative use in the United States. Successful pumpkin production requires the use of cultivars that yield well and produce pumpkins of the size, shape, color, and quality demanded by the market. Genetic resistance to the fungal disease powdery mildew is present in some varieties. This trial was designed to evaluate performance of pumpkin varieties in northern Indiana with and without treatment for powdery mildew. The trial included eight jack-o-lantern size pumpkins, …

Sugar-Enhanced Sweet Corn Cultivar Evaluation For Northern Indiana, 2009, Elizabeth T. Maynard

Midwest Vegetable Trial Reports

Indiana growers harvested sweet corn for fresh market sales from 5,400 acres in 2008, with an average yield of 70 cwt/A (166 crates or 3.5 tons per acre) and total value of $11.7 million (USDA-NASS Indiana Agricultural Statistics, 2008-2009). Indiana ranks 17th among states for production of fresh market sweet corn. The 2007 USDA Ag Census reported 603 Indiana farms producing sweet corn for fresh markets and 51 farms selling to processors. Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bicolor corn is most commonly grown. Varieties with improved eating quality are of …

Morphogenesis And Tissue Culture Studies Of Gerbera Jamesonii Bolus Ex. Hook F., Nor Azlina Hasbullah

Student Works (2010-2019)

In vitro regeneration of Gerbera jamesonii Bolus ex. Hook f. was successfully carried out in this study. Petiole and leaf explants from 8-week-old aseptic seedlings were used as source of explants. Different response was observed when different explants were cultured on the culture media. MS (Murashige and Skoog, 1962) supplemented with 2.0 mg/l BAP and 0.5 mg/l NAA was identified as the optimum medium for shoot regeneration, while petiole explant was identified as the most responsive explant in this study. Petiole explants regenerated shoots (94.3 } 2.5 %) and produced 9.3 } 0.6 shoots per explant. MS basal medium was …

Effects Of Plant Growth Regulators And Physiological Stresses On The Growth Of Bougainvillea Spp., Mohammed Saifuddin

Student Works (2010-2019)

This study was aimed to investigate the effects of physiological stresses such as pruning, shading, phloemic stress and plant hormones such as gibberellic acid (GA3) and naphthalene acetic acid (NAA) on the growth, development and flowering process of bougainvillea plants. The plants (Bougainvillea glabra and Bougainvillea spectabilis) were grown under prevailing conditions (temperature 21-32°C, maximum PAR 2100 μE m-2 s-1 and relative humidity of 60-90%) using completely randomized design. Field experiments as well as biochemical analysis were carried out at the Plant Physiology Garden and the Plant Physiology Laboratory, Institute of Biological Sciences, University of Malaya. Results on frequent pruning …

Sfa Gardens Newsletter, Winter 2010, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Newsletters

No abstract provided.