Qtl For Seed Protein And Amino Acids In The
Benning × Danbaekkong Soybean Population,
2015
University of Georgia
Qtl For Seed Protein And Amino Acids In The Benning × Danbaekkong Soybean Population, C. V. Warrington, H. Abdel‑Haleem, D. L. Hyten, P. B. Cregan, J. H. Orf, A. S. Killam, N. Bajjalieh, Z. Li, H. R. Boerma
Department of Agronomy and Horticulture: Faculty Publications
Soybean, rather than nitrogen-containing forages, is the primary source of quality protein in feed formulations for domestic swine, poultry, and dairy industries. As a sole dietary source of protein, soybean is deficient in the amino acids lysine (Lys), threonine (Thr), methionine (Met), and cysteine (Cys). Increasing these amino acids would benefit the feed industry. The objective of the present study was to identify quantitative trait loci (QTL) associated with crude protein (cp) and amino acids in the ‘Benning’ × ‘Danbaekkong’ population. The population was grown in five southern USA environments. Amino acid concentrations as a fraction of cp (Lys/cp, Thr/cp, …
First Approximations Of Prescribed Fire Risks
Relative To Other Management Techniques
Used On Private Lands,
2015
University of Nebraska-Lincoln
First Approximations Of Prescribed Fire Risks Relative To Other Management Techniques Used On Private Lands, Dirac L. Twidwell, Carissa L. Wonkka, Michael T. Sindelar, John R. Weir
Department of Agronomy and Horticulture: Faculty Publications
Fire is widely recognized as a critical ecological and evolutionary driver that needs to be at the forefront of land management actions if conservation targets are to be met. However, the prevailing view is that prescribed fire is riskier than other land management techniques. Perceived risks associated with the application of fire limits its use and reduces agency support for prescribed burning in the private sector. As a result, considerably less cost-share support is given for prescribed fire compared to mechanical techniques. This study tests the general perception that fire is a riskier technique relative to other land management options. …
A Genomic Selection Index Applied To Simulated
And Real Data,
2015
Biometrics and Statistics Unit, International Maize and Wheat Improvement Center (CIMMYT), Mexico
A Genomic Selection Index Applied To Simulated And Real Data, J. Jesus Ceron-Rojas, Jose Crossa, Vivi N. Arief, Kaye Basford, Jessica Rutkoski, Diego Jarquin, Gregorio Alvarado, Yoseph Beyene, Kassa Semagn, Ian Delacy
Department of Agronomy and Horticulture: Faculty Publications
A genomic selection index (GSI) is a linear combination of genomic estimated breeding values that uses genomic markers to predict the net genetic merit and select parents from a nonphenotyped testing population. Some authors have proposed a GSI; however, they have not used simulated or real data to validate the GSI theory and have not explained how to estimate the GSI selection response and the GSI expected genetic gain per selection cycle for the unobserved traits after the first selection cycle to obtain information about the genetic gains in each subsequent selection cycle. In this paper, we develop the theory …
Proteomic Profiling Of Maize Opaque Endosperm Mutants
Reveals Selective Accumulation Of Lysine-Enriched Proteins,
2015
University of Nebraska-Lincoln
Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding
Department of Agronomy and Horticulture: Faculty Publications
Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such as protein transport and folding, amino acid biosynthesis, …
Tonoplast Sugar Transporters (Sbtsts) Putatively Control
Sucrose Accumulation In Sweet Sorghum Stems,
2015
University of Missouri
Tonoplast Sugar Transporters (Sbtsts) Putatively Control Sucrose Accumulation In Sweet Sorghum Stems, Saadia Bihmidine, Benjamin T. Julius, Ismail M. Dweikat, David M. Braun
Department of Agronomy and Horticulture: Faculty Publications
Carbohydrates are differentially partitioned in sweet versus grain sorghums. While the latter preferentially accumulate starch in the grain, the former primarily store large amounts of sucrose in the stem. Previous work determined that neither sucrose metabolizing enzymes nor changes in Sucrose transporter (SUT) gene expression accounted for the carbohydrate partitioning differences. Recently, 2 additional classes of sucrose transport proteins, Tonoplast Sugar Transporters (TSTs) and SWEETs, were identified; thus, we examined whether their expression tracked sucrose accumulation in sweet sorghum stems. We determined 2 TSTs were differentially expressed in sweet vs. grain sorghum stems, likely underlying the massive difference in sucrose …
Differential Expression Analysis In Rna-Seq By
A Naive Bayes Classifier With Local Normalization,
2015
University of Nebraska-Lincoln
Differential Expression Analysis In Rna-Seq By A Naive Bayes Classifier With Local Normalization, Yongchao Dou, Xiaomei Guo, Lingling Yuan, David R. Holding, Chi Zhang
Department of Agronomy and Horticulture: Faculty Publications
To improve the applicability of RNA-seq technology, a large number of RNA-seq data analysis methods and correction algorithms have been developed. Although these new methods and algorithms have steadily improved transcriptome analysis, greater prediction accuracy is needed to better guide experimental designs with computational results. In this study, a new tool for the identification of differentially expressed genes with RNA-seq data, named GExposer, was developed. This tool introduces a local normalization algorithm to reduce the bias of nonrandomly positioned read depth. The naive Bayes classifier is employed to integrate fold change, transcript length, and GC content to identify differentially expressed …
The Effect Of Turning Frequency On In-Vessel Compost Processing And Quality,
2015
University of Arkansas, Fayetteville
The Effect Of Turning Frequency On In-Vessel Compost Processing And Quality, Paige E. Boyle, Mary C. Savin, Lisa S. Wood
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Composting can contribute to the zero waste initiative on the University of Arkansas (UA) campus. In-vessel systems like Earth Tubs™ are purported to provide better control of temperature and moisture during the composting process. Turning materials helps facilitate microbial activity and thermophilic composting. The goal of this research was to determine if turning frequency affects processing or final quality of compost made with pre- and post-consumer food waste feedstock and a wood chip bulking agent. Turning frequencies (treatment) of 3 days/week and 7 days/ week were evaluated simultaneously throughout three sequential runs. Temperature, pH, electrical conductivity (EC), and moisture content …
Dispersal: A Multidisciplinary Investigation Of Plant Life,
2015
Virginia Commonwealth University
Dispersal: A Multidisciplinary Investigation Of Plant Life, Alexandra E. Arzt
Theses and Dissertations
Using plants as a basis for exploring the interstices between the human and nonhuman, this thesis investigates ideas of awareness, intelligence, deep time, animism, and the fluctuating human perception of the agency of Nature. It outlines environmental art practices since the 1950s involving vegetal life. In addition, the paper provides a critical analysis of plant perception of Jakob von Uexküll’s work and theories of vital materialism and “critical plant studies” while noting recent studies in plant neurobiology. In my work, plants become active participants via their movement, seeding, and smell. This study takes the form of imitation, purposeful symbiosis, anthropomorphism, …
The Influence Of Production Practices, Tillage, And Endophytic Bacteria On Bell Pepper Productivity And Physiology Under Different Irrigation Regimes,
2015
University of Kentucky
The Influence Of Production Practices, Tillage, And Endophytic Bacteria On Bell Pepper Productivity And Physiology Under Different Irrigation Regimes, Zheng Wang
Theses and Dissertations--Plant and Soil Sciences
To evaluate the strip tillage in organic bell pepper (Capsicum annuum L.) production as an integrated system for sustainable vegetable cropping two-years of field trials were conducted in 2011 and 2012. The field trials were conducted to determine the viability of strip tillage in conventional and organic bell pepper production systems by comparing plant growth, water status, and fruit yield to plastic mulch grown plants application under different irrigation regimes. The two-year field data demonstrated that organic pepper with strip tillage application was a viable combination that produced comparable yield to conventional plastic mulch system and utilized water more …
Conservation Agriculture In Kentucky: Investigating Nitrogen Loss And Dynamics In Corn Systems Following Wheat And Hairy Vetch Cover Crops,
2015
University of Kentucky
Conservation Agriculture In Kentucky: Investigating Nitrogen Loss And Dynamics In Corn Systems Following Wheat And Hairy Vetch Cover Crops, Rebecca Erin Shelton
Theses and Dissertations--Plant and Soil Sciences
Unintentional nitrogen (N) loss from agroecosystems produces greenhouse gases, induces eutrophication, and is costly for farmers; therefore, adoption of conservation agricultural management practices, such as no-till and cover cropping, has increased. This study assessed N loss via leaching, NH3 volatilization, N2O emissions, and N retention in plant and soil pools of corn conservation agroecosystems across a year. Three systems were evaluated: 1) an unfertilized organic system with cover crops Vicia villosa, Triticum aestivum, or a mix of the two; 2) an organic system with a Vicia cover crop employing three fertilization schemes (0 N, organic N, …
An Assessment Of Biochar Amended Soilless Media For Nursery Propagation Of Northern Rocky Mountain Native Plants,
2015
The University Of Montana
An Assessment Of Biochar Amended Soilless Media For Nursery Propagation Of Northern Rocky Mountain Native Plants, Clarice P. Matt
Graduate Student Theses, Dissertations, & Professional Papers
A study was conducted to better inform nursery practitioners of the potential benefits of biochar application in common nursery media for native plant propagation. Biochar – a carbon-rich, recalcitrant charred organic co-product of the bioenergy pyrolysis process – has emerged as a promising potential replacement for various components of soilless media, namely peat, perlite, and vermiculite, in nursery seedling propagation. A strong conceptual basis exists for biochar as a nursery media amendment, but empirical data on biochar-based plant propagation is limited. This greenhouse study examined the effects of biochar displacement of standard soilless nursery media at rates of 0%, 15%, …
Assessment Of Rice Self-Sufficiency In 2025 In Eight African Countries,
2015
Wageningen University
Assessment Of Rice Self-Sufficiency In 2025 In Eight African Countries, P. A.J. Van Oort, K. Saito, E. Amovin-Assagba, Lenny G.J. Van Bussel, Justin Van Wart, Hugo De Groot, Martin K. Van Ittersum, Kenneth Cassman, M. C.S. Wopereis
Department of Agronomy and Horticulture: Faculty Publications
Most African countries are far from self-sufficient in meeting their rice consumption; in eight countries the production: consumption ratio, ranged from 0.16 to 1.18 in 2012. We show that for the year 2025, with population growth, diet change and yield increase on existing land (intensification), countries cannot become fully self-sufficient in rice. This implies that for the future, a mixture of area expansion and imports will be needed on top of yield gap closure. Further research is needed for identification of most suitable new land for rice area expansion and areas that should be protected.
Selective Genotyping For Marker Assisted Selection Strategies For Soybean Yield Improvement,
2015
Clemson University
Selective Genotyping For Marker Assisted Selection Strategies For Soybean Yield Improvement, Benjamin D. Fallen, Fred L. Allen, Dean A. Kopsell, Arnold M. Saxton, Leah Mchale, J. Grover Shannon, Stella K. Kantartzi, Andrea J. Cardinal, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone
Department of Agronomy and Horticulture: Faculty Publications
Using molecular markers in soybean [Glycine max (L.) Merr.] has lead to the identification of major loci controlling quantitative and qualitative traits that include: disease resistance, insect resistance and tolerance to abiotic stresses. Yield has been considered as one of the most important quantitative traits in soybean breeding. Unfortunately, yield is a very complex trait and most yield quantitative trait loci (QTL) that have been identified have had only limited success for marker assisted selection (MAS). The objective of this study was to identify QTL associated with soybean seed yield in preliminary yield trials grown in different environments and …
Using A Simple Leaf Color Chart To Estimate Leaf
And Canopy Chlorophyll A Content In Maize (Zea
Mays),
2015
University of Nebraska-Lincoln
Using A Simple Leaf Color Chart To Estimate Leaf And Canopy Chlorophyll A Content In Maize (Zea Mays), Anthony Nguy-Robertson, Yi Peng, Timothy Arkebauer, David Scoby, James Schepers, Anatoly Gitelson
Department of Agronomy and Horticulture: Faculty Publications
This study utilized a leaf color chart (LCC) to characterize the variation in leaf chlorophyll and estimate canopy chlorophyll in maize (Zea mays). The LCC consisted of four levels of greenness and was used to sort maize leaves in 2011 for three fields near Mead, Nebraska, USA. Leaf chlorophyll content for each color chart class was determined using two leaf-level sensors. The variation within each LCC class was reasonable (CV < 56%). The darkest color class predominated and indicated adequate fertilization rates using a SPAD. Canopy chlorophyll content was estimated using destructively measured leaf area index (LAI) and the LCC. This approach was verified with a method utilizing canopy reflectance collected by both satellite imagery and a four-band radiometer. The error between the two methods was reasonable (RMSE = 0.55-0.88 g m-2; CV = 25.6-50.4%), indicating that both leaf and canopy chlorophyll can be estimated cheaply without a wet lab or field-based sensors.
Transgenic Wheat Expressing
A Barley Udp-Glucosyltransferase Detoxifies
Deoxynivalenol And Provides High Levels
Of Resistance To Fusarium Graminearum,
2015
University of Minnesota
Transgenic Wheat Expressing A Barley Udp-Glucosyltransferase Detoxifies Deoxynivalenol And Provides High Levels Of Resistance To Fusarium Graminearum, Xi Li, Sanghyun Shin, Shane Heinen, Ruth Dill-Macky, Franz Berthiller, Natalya Nersesian, Thomas Clemente, Susan Mccormick, Gary J. Muehbauer
Department of Agronomy and Horticulture: Faculty Publications
Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a devastating disease of wheat that results in economic losses worldwide. During infection, F. graminearum produces trichothecene mycotoxins, including deoxynivalenol (DON), that increase fungal virulence and reduce grain quality. Transgenic wheat expressing a barley UDP-glucosyltransferase (HvUGT13248) were developed and evaluated for FHB resistance, DON accumulation, and the ability to metabolize DON to the less toxic DON-3-O-glucoside (D3G). Pointinoculation tests in the greenhouse showed that transgenic wheat carrying HvUGT13248 exhibited significantly higher resistance to disease spread in the spike (type II resistance) compared with nontransformed controls. Two transgenic …
Understanding Rice Adaptation To Varying
Agro-Ecosystems: Trait Interactions And
Quantitative Trait Loci,
2015
International Rice Research Institute
Understanding Rice Adaptation To Varying Agro-Ecosystems: Trait Interactions And Quantitative Trait Loci, Shalabh Dixit, Alexandre Grondin, Cheng-Ruei Lee, Amelia Henry, Thomas-Mitchell Olds, Arvind Kumar
Department of Agronomy and Horticulture: Faculty Publications
Background: Interaction and genetic control for traits influencing the adaptation of the rice crop to varying environments was studied in a mapping population derived from parents (Moroberekan and Swarna) contrasting for drought tolerance, yield potential, lodging resistance, and adaptation to dry direct seeding. A BC2F3-derived mapping population for traits related to these four trait groups was phenotyped to understand the interactions among traits and to map and align QTLs using composite interval mapping (CIM). The study also aimed to identify QTLs for the four trait groups as composite traits using multivariate least square interval mapping (MLSIM) …
Overexpression Of Sbmyb60 Impacts Phenylpropanoid
Biosynthesis And Alters Secondary Cell Wall Composition In
Sorghum Bicolor,
2015
University of Nebraska-Lincoln
Overexpression Of Sbmyb60 Impacts Phenylpropanoid Biosynthesis And Alters Secondary Cell Wall Composition In Sorghum Bicolor, Erin D. Scully, Tammy Gries, Gautam Sarath, Nathan A. Palmer, Lisa Baird, Michelle J. Serapiglia, Bruce S. Dien, Akwasi A. Boateng, Zhengxiang Ge, Deanna L. Funnell-Harris, Paul Twigg, Thomas E. Clemente, Scott E. Sattler
Department of Agronomy and Horticulture: Faculty Publications
The phenylpropanoid biosynthetic pathway that generates lignin subunits represents a significant target for altering the abundance and composition of lignin. The global regulators of phenylpropanoid metabolism may include MYB transcription factors, whose expression levels have been correlated with changes in secondary cell wall composition and the levels of several other aromatic compounds, including anthocyanins and flavonoids. While transcription factors correlated with downregulation of the phenylpropanoid biosynthesis pathway have been identified in several grass species, few transcription factors linked to activation of this pathway have been identified in C4 grasses, some of which are being developed as dedicated bioenergy feedstocks. In …
A Dedicated Type Ii Nadph Dehydrogenase Performs The
Penultimate Step In The Biosynthesis Of Vitamin K1 In
Synechocystis And Arabidopsis,
2015
University of Nebraska–Lincoln
A Dedicated Type Ii Nadph Dehydrogenase Performs The Penultimate Step In The Biosynthesis Of Vitamin K1 In Synechocystis And Arabidopsis, Abdelhak Fatihi, Scott Latimer, Stefan Schmollinger, Anna Block, Patrick Dussault, Wim F.J. Vermaas, Sabeeha S. Merchant, Gilles J. Basset
Department of Agronomy and Horticulture: Faculty Publications
Mutation of Arabidopsis thaliana NAD(P)H DEHYDROGENASE C1 (NDC1; At5g08740) results in the accumulation of demethylphylloquinone, a late biosynthetic intermediate of vitamin K1. Gene coexpression and phylogenomics analyses showed that conserved functional associations occur between vitamin K biosynthesis and NDC1 homologs throughout the prokaryotic and eukaryotic lineages. Deletion of Synechocystis ndbB, which encodes for one such homolog, resulted in the same defects as those observed in the cyanobacterial demethylnaphthoquinone methyltransferase knockout. Chemical modeling and assay of purified demethylnaphthoquinone methyltransferase demonstrated that, by virtue of the strong electrophilic nature of S-adenosyl-Lmethionine, the transmethylation of the demethylated precursor …
Aquaporins Contribute To Aba-Triggered Stomatal Closure
Through Ost1-Mediated Phosphorylation,
2015
University of Nebraska-Lincoln
Aquaporins Contribute To Aba-Triggered Stomatal Closure Through Ost1-Mediated Phosphorylation, Alexandre Grondin, Olivier Rodrigues, Lionel Verdoucq, Sylvain Merlot, Nathalie Leonhardt, Christophe Maurel
Department of Agronomy and Horticulture: Faculty Publications
Stomatal movements in response to environmental stimuli critically control the plant water status. Although these movements are governed by osmotically driven changes in guard cell volume, the role of membrane water channels (aquaporins) has remained hypothetical. Assays in epidermal peels showed that knockout Arabidopsis thaliana plants lacking the Plasma membrane Intrinsic Protein 2;1 (PIP2;1) aquaporin have a defect in stomatal closure, specifically in response to abscisic acid (ABA). ABA induced a 2-fold increase in osmotic water permeability (Pf) of guard cell protoplasts and an accumulation of reactive oxygen species in guard cells, which were both abrogated in …
Selection For Silage Yield And Composition Did Not
Affect Genomic Diversity Within The Wisconsin
Quality Synthetic Maize Population,
2015
University of Nebraska-Lincoln
Selection For Silage Yield And Composition Did Not Affect Genomic Diversity Within The Wisconsin Quality Synthetic Maize Population, Aaron Lorenz, Timothy M. Beissinger, Renato Rodrigues Silva, Natalia De Leon
Department of Agronomy and Horticulture: Faculty Publications
Maize silage is forage of high quality and yield, and represents the second most important use of maize in the United States. The Wisconsin Quality Synthetic (WQS) maize population has undergone five cycles of recurrent selection for silage yield and composition, resulting in a genetically improved population. The application of high-density molecular markers allows breeders and geneticists to identify important loci through association analysis and selection mapping, as well as to monitor changes in the distribution of genetic diversity across the genome. The objectives of this study were to identify loci controlling variation for maize silage traits through association analysis …
