Botany Commons^™

Physiological And Transcriptomic Responses Of Two Artemisia Californica Populations To Drought: Implications For Restoring Drought-Resilient Native Communities, Hagop S. Atamian Dr., Jennifer L. Funk

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

As climate change brings drier and more variable rainfall patterns to many arid and semi-arid regions, land managers must re-assemble appropriate plant communities for these conditions. Transcriptome sequencing can elucidate the molecular mechanisms underlying plant responses to changing environmental conditions, potentially enhancing our ability to screen suitable genotypes and species for restoration. We examined physiological and morphological traits and transcriptome sequences of coastal and inland populations of California sagebrush (Artemisia californica), a critical shrub used to restore coastal sage scrub vegetation communities, grown under low and high rainfall environments. The populations are located approximately 36 km apart but …

Late Postemergence Glufosinate-Based Programs For Glyphosate-Resistant Palmer Amaranth Control In Dicamba/Glufosinate/Glyphosate-Resistant Soybean, Vipan Kumar, Jatinder S. Aulakh, Rui Liu, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Glyphosate-resistant (GR) Palmer amaranth (Amaranthus palmeri S. Watson) is widespread in the Central Great Plains. Introduction of newly developed dicamba/glufosinate/glyphosate (DGG)-resistant soybean varieties allows postemergence (POST) applications of dicamba and glufosinate for in-season control of GR Palmer amaranth. Limited information exists on the effectiveness of glufosinate applied late-POST for tall (70–90 cm) GR Palmer amaranth control in DGG-resistant soybean. The objectives of this study were to (1) determine the effectiveness of late-POST glufosinate-based programs for GR Palmer amaranth control, and (2) determine the impact of those programs on soybeans grain yields. Ten glufosinatebased programs were tested in a field …

A Leaf-Level Spectral Library To Support High-Throughput Plant Phenotyping: Predictive Accuracy And Model Transfer, Nuwan K. Wijewardane, Huichun Zhang, Jinliang Yang, James C. Schnable, Daniel P. Schachtman, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

Leaf-level hyperspectral reflectance has become an effective tool for high-throughput phenotyping of plant leaf traits due to its rapid, low-cost, multi-sensing, and non-destructive nature. However, collecting samples for model calibration can still be expensive, and models show poor transferability among different datasets. This study had three specific objectives: first, to assemble a large library of leaf hyperspectral data (n=2460) from maize and sorghum; second, to evaluate two machine-learning approaches to estimate nine leaf properties (chlorophyll, thickness, water content, nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur); and third, to investigate the usefulness of this spectral library for predicting external datasets …

Decadal Patterns Of Adaptation And Trait Variation Of The Dominant Prairie Grass Across The Midwest Rainfall Gradient And Response To Drought, Jack Sytsma

2023 Midwest Ecology & Evolution Conference

Frequent droughts are predicted to impact grasslands but intraspecific variation in response to drought is not well-known. Big bluestem (Andropogon gerardii) is a grass distributed across the Midwest rainfall gradient (MAP500-1200mm/yr) giving rise to climate-adapted ecotypes. This dominant grass is important for cattle forage and conservation. Reciprocal gardens were established in 2009 in ecological communities (driest to wettest: Colby, Hays, Manhattan, KS to Carbondale IL) and measured for over a decade. Rainfall was reduced using rainout shelters in three sites. Cover and biomass were monitored in plots over time, and height, width, photosynthetic rate, SPAD, and reproductive phenology …

Documenting Plant Biodiversity At The Eastern Michigan University Owned Parsons Center For Arts And Sciences, Emily R. Edwards Mrs.

2023 Midwest Ecology & Evolution Conference

Eastern Michigan University (EMU) owns an 86-acre property in northwest Michigan called the Parsons Center for Arts and Sciences. No botanical research has been done at the Center. A comprehensive plant species list will provide important resources for EMU students, all Parsons users (e.g., artists and community members), and county biologists. This research aims to collect and identify as many plant species at the site as possible and create two online, publicly available databases of the plant list and collection. Such a collection will bring awareness to the Parsons Center and add to its botanical value.

Initial field-work was conducted …

A Tale Of Two Scents: Scent Preferences Of Naïve And Experienced Pollinators, Scout N. Steinhiser

2023 Midwest Ecology & Evolution Conference

Mounting evidence of pollinator decline and its repercussions on agriculture and plant diversity means that scientists must work to understand the details of plant-pollinator interactions. Though there are many facets to the relationship between plants and pollinators, one major mediator of this relationship is scent. As much is already known about the scent make-up of Narcissus and its pollinator partners, it is a great model system for additional research. This is also true for the insect species Vanessa cardui, a generalist pollinator found worldwide that has most likely come into contact with Narcissus and its scent. The objective of this …

Trait Variation In Bryophytes At Karst Cave Entrances, Chenoa Briggs

2023 Midwest Ecology & Evolution Conference

Cave entrances are critical ecosystems in their role as ecotones between the interior cave environment and the surrounding external environment. Bryophytes are highly sensitive to environmental changes and many species found at caves are specialists to those habitats. This study aims to identify how functional traits of bryophytes are affected by the cave environment. Bryophytes and environmental variables were sampled from six cave entrances. Functional trait data was collected from literature. The data from this study supplies information on the functional traits of bryophytes and can provide insight into the effects of environmental pressures on community assembly.

Geographic Analysis Of Sex Chromosome Polymorphism In The Virginian Wild Strawberry, Trezalka A. Budinsky

2023 Midwest Ecology & Evolution Conference

The evolution of sex chromosomes is a ubiquitous and essential diversifying mechanism in eukaryotes. Yet early stages of sex-chromosome differentiation are poorly understood. The subdioecious octoploid, Fragaria virginiana, provides a rare opportunity to study the beginnings of sex chromosome evolution due to its young, polymorphic ZW sex system. Females are ZW and males/hermaphrodites are ZZ. F. virginiana has a small sex-determining region (SDR) located on the female W chromosome that has undergone three transposition events between the strawberry's four subgenomes, resulting in three SDR haplotypes: alpha (the result of a first transposition and closest to a pre-sex chromosome ancestral …

The Influence Of Cover Crops On Plant-Soil Microbe Interactions Of Native Prairie Forbs, Josh C. Poland

2023 Midwest Ecology & Evolution Conference

Tallgrass prairies are abundant and rich habitats where soil microbial communities frequently interact with native grass and forb species. Cover crops increase available soil nutrients and promote healthy soil microbial communities in agriculture, and therefore may provide similar benefits to restoration. A prairie restoration in Madison Co, IL was initiated in autumn 2021 using areas with two different cover crop types, NLS Soil Health Mix and Winter Oat, as well as areas with no cover crops. A greenhouse project exposed five native forb species (Monarda fistulosa, Rudbeckia hirta, Pycnanthemum virginianum, Desmodium canadensis (legume), and Asclepias incarnata …

Bryophytes At Cave Entrances Across A Range Of Anthropogenic Disturbance, Morghan Mccool

2023 Midwest Ecology & Evolution Conference

Cave entrances are critical ecosystems in their role as ecotones between the interior cave environment and the surrounding external environment. Bryophytes serve as ecosystem engineers of cave entrance microhabitats. This study piloted a method to quantify anthropogenic disturbance unique to cave entrances and characterize the bryophyte assemblages at caves in urban and other natural areas. Bryophytes and environmental variables were sampled from cave entrances; bryophytes were identified, alpha and beta diversity values were computed. So far, 144 species of bryophytes, 36 of which were state records, have been identified across 6 sites. By improving knowledge of bryophyte communities, park managers …

A Phylogenetic Evaluation Of Proposed Segregates Of A. Canadense, Brett Lambert

2023 Midwest Ecology & Evolution Conference

Brett Lambert- 1^st Year Master’s Student at The Ohio State University

Department of Evolution, Ecology, and Organismal Biology (Freudenstein Lab)

Title: A phylogenetic evaluation of proposed segregates of Asarum canadense

Abstract: The wild gingers (Asarum: Aristolochiaceae) are rhizomatous forbs native to Europe, Asia, and North America. A member of subgenus Asarum (A. canadense) is currently circumscribed as containing two varieties, A. canadense var. reflexum and A. canadense var. acuminatum. These varieties have previously been recognized as distinct species based on morphological variation. This project will evaluate variation across A. canadense using molecular (maximum likelihood and …

Calceolaria Nivalis Subsp. Lanatifolia, A New Subspecies Of Calceolariaceae From Northern Peru, Pamela Puppo

Biological Sciences Faculty Research

A new subspecies of Calceolaria nivalis, C. nivalis subsp. lanatifolia is described and illustrated. The new subspecies was collected in Uchumarca district, department of La Libertad, Peru, between 2300–3850 m elevation. Calceolaria nivalis subsp. lanatifolia is characterized by the lanate indumentum in the lower surface of the leaves, villous petioles, peduncles, and pedicels, and sepals with a strigose margin internally.

Effects Of Heavy Metal Uptake On Growth, Chlorophyll Content, And Calcium Oxalate Crystals In Lemna Minor (Duckweed), Olivia F. Miller

Undergraduate Honors Thesis Projects

Lemna minor L., duckweed, is a common aquatic plant used for the phytoremediation of wastewater. Heavy metal contamination poses a significant issue, and numerous studies have been conducted on the efficiency of L. minor’s hyperaccumulation ability of these metals. Calcium oxalate crystals are an important aspect of the growth and development of L. minor, but how they are influenced by the uptake of heavy metal has not been extensively studied. This study aims to determine the effects that lead, cadmium, and copper have on the formation of calcium oxalate crystals, vegetative growth, and chlorophyll a and b content on L. …

Mycorrhizal Fungi And Reforestation In An Eastern Lowland Rainforest Of Madagascar, Sophia Gibby

Independent Study Project (ISP) Collection

Most terrestrial plants worldwide make associations with mycorrhizal fungi, yet the fungal diversity of Madagascar is vastly unknown. This research project is a preliminary investigation into the mycorrhizal status of 19 tree species in and near Ranomafana National Park (RNP), a lowland tropical forest on the southeast coast of Madagascar. Arbuscular mycorrhizal (AM) colonization in these species was studied in root samples under the microscope in the laboratory of Centre ValBio (CVB). The degree of AM infection was assessed for three study areas: one directly adjacent to a protected area and two reforestation sites supported by CVB. For the 19 …

Understory Epiphyte Hydrology: Analyzing Water Storage Capacity Of Epiphytes Along An Elevational Gradient In Western Ecuadorian Cloud Forest, Angelina Dodge

Independent Study Project (ISP) Collection

Epiphytes are defined as plants that grow on a host, often another plant, and acquire their nutrients and water from the atmosphere. As such is true, epiphyte composition is largely dependent upon atmospheric and thus climactic conditions. Due to high levels of atmospheric water availability that results from the frequent presence of mist, clouds, and high humidity, epiphytic plants grow and thus store water within the above ground region of the Andean cloud forest at levels higher than in any other ecosystem found in Ecuador. This study was done in hopes of revealing any trends of epiphytic water storage capacity …

Variation In The Growth Parameters And Biomass Of Rhizophora Mangle Seedlings With Distances From Playa Estrella, Bocas Del Toro, Panama, Thiny Tep

Independent Study Project (ISP) Collection

Mangrove is a salt-tolerant, intertidal, tropical tree or shrub and make up a rich community of various organism. On the Caribbean coast of Panama, in Bocas del Toro, mangrove forests cover 28 km² and are dominated by R. mangle, followed by L. racemosa and A. germinans. Simultaneously, Isla Colón, the most populated and developed among all islands in the Bocas del Toro Archipelago, is a tourist center. Unfortunately, tourism comes at the price of environmental degradation via alteration of natural habitats, solid and wastewater pollution. Therefore, this study aims to gain a preliminary understanding on how the …

Experimentation On Nebraska Farms For Sustaining Soil Health Management, Fernanda Souza Krupek

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Soil health management practices have increasingly been promoted across US agroecosystems to address many interrelated environmental and economic food system challenges. Sustaining conservation behavior – through farmer’s adoption and continued use of practices – is key for achieving many soil health-related intended social-ecological benefits. Using a range of scientific methods, from lab-based experiments to on-farm research to farmer interviews, the overall objective of this dissertation research was to explore soil and human dimension considerations to design farming and knowledge transfer systems for sustaining soil health management in the US Midwest. In a multivariate analysis of ten on-farm research sites, we …

Arkansas Cotton Variety Test 2022, F. Bourland, A. Beach, B. Milano, B. Guest, C. Kennedy, L. Martin, B. Robertson

Arkansas Agricultural Experiment Station Research Series

The primary goal of the Arkansas Cotton Variety Test is to provide unbiased data regarding the agronomic performance of cotton varieties and advanced breeding lines in the major cotton-growing areas of Arkansas. This information helps seed companies establish marketing strategies and assists producers in choosing varieties to plant. These annual evaluations will then facilitate the inclusion of new, improved genetic material in Arkansas cotton production. Adaptation of varieties is determined by evaluating the lines at five University of Arkansas System Division of Agriculture research sites (Manila, Keiser, Judd Hill, Marianna, and Rohwer). The 2022 tests at Rohwer were adversely affected …

Biocultural Diversity Of Medicine In Tsum Valley, Ashira Weinreich

Independent Study Project (ISP) Collection

Health is intrinsically connected to biodiversity in the Himalayan mountains of Tsum Valley. Medicinal plants have historically been integral to primary health care in this region. The main objectives of this paper are to explore the role of biocultural diversity on perceptions of health and to demonstrate that medicinal plant knowledge contributes to health sovereignty in times of cultural and environmental change. The nutritional and medicinal significance of plants were examined in the daily lives of villagers. Semi-structured interviews were conducted from April to May 2023. Preliminary results are analyzed ethnographically. Interviews and participant observation will provide a rich understanding …

Evaluation Of Free Radical Scavenging  Activity Of Sauce And Formulation Of Tamarindus Indica (L.) And Prunus Domestica (L.) Fruits, Zulnoreen Sarfaraz, Mehmooda Munazir, Mubashrah Munir, Asma Ahmed, Rehana Badar, Ayesha Tahir

Journal of Bioresource Management

The formulation and sauce of Tamarindus indica  (L.) and Prusnus  dometica (L.) are very popular recipes across globe. But, not even a single study existed on free-radical scavenging activity (FRSA) of afore-mentioned recipes. So, the present study was designed to bridge this gap of information. The FRSA of individual samples were also determined and compared with that of combinations of both. The highest value for FRSA among all samples was that of T. indica (158.9732 ± 12.658 % at 100 μg ml^-1). It was followed by that of individual extract of P. domestica fruit (96.647 ± 0.554 %). …