Phylloplanins: Novel Antifungal Proteins On Plant Leaf Surfaces,
2010
University of Kentucky
Phylloplanins: Novel Antifungal Proteins On Plant Leaf Surfaces, Ryan William Shepherd
University of Kentucky Doctoral Dissertations
Secreted surface proteins are an innate immune defense component employed by animals to inhibit invading microbes. Surface proteins have not been documented in plants, even though the aerial leaf surface, or phylloplane, is a major site of pathogen ingress. We have discovered novel proteins, termed phylloplanins, which accumulate on leaf surfaces of Nicotiana tabacum, and we have isolated the gene Phylloplanin that is unique in gene databases. Natural and E. coli-expressed phylloplanins inhibit spore germination and limit leaf infection by the oomycete pathogen Peronospora tabacina.
We investigated the site of phylloplanin biosynthesis using biochemical techniques. These techniques …
Whole Genome Wide Expression Profiles Of Vitis Amurensis Grape Responding To Downy Mildew By Using Solexa Sequencing Technology,
2010
China Agricultural University
Whole Genome Wide Expression Profiles Of Vitis Amurensis Grape Responding To Downy Mildew By Using Solexa Sequencing Technology, Jiao Wu, Yali Zhang, Huiqin Zhang, Hong Huang, Kevin M. Folta, Jiang Lu
School of Information Faculty Publications
Background: Downy mildew (DM), caused by pathogen Plasmopara viticola (PV) is the single most damaging disease of grapes (Vitis L.) worldwide. However, the mechanisms of the disease development in grapes are poorly understood. A method for estimating gene expression levels using Solexa sequencing of Type I restriction-endonuclease-generated cDNA fragments was used for deep sequencing the transcriptomes resulting from PV infected leaves of Vitis amurensis Rupr. cv. Zuoshan-1. Our goal is to identify genes that are involved in resistance to grape DM disease.
Results: Approximately 8.5 million (M) 21-nt cDNA tags were sequenced in the cDNA library derived from PV pathogen-infected …
