Agricultural Science Commons^™

Superweeds And Suspect Seeds: Does The Genetically-Engineered Crop Deregulation Process Put American Agriculture At Risk, Margaret Sova Mccabe

Law Faculty Scholarship

The federal government’s regulatory approach to genetically engineered (GE) crops, known as “The Framework”, is now twenty-five years old. Despite two and half decades of a consistent regulatory regime, GE crop and food regulation remains controversial. This article suggests that regulatory science and its tenets of independence, transparency, and public science should guide reforms of The Framework so that it is an efficient and reliable regulatory system. The article has four parts: 1) it provides a brief overview of the history of GE crop regulation; 2)it describes the key attributes of The Framework and related regulatory documents, with particular focus …

Attraction Of Stomoxys Sp. To Various Fruits And Flowers In Mali, Günter Müller, Jerome Hogsette, University Of Miami Beier, University Of Bamako, M.B. Toure, M.M. Traore, S. Bah, S. Doumbia, Y. Schlein

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The attraction of three Stomoxys species to 26 fruits and 26 flowers of different plant species was investigated in two different sites in Mali during 2008. Stomoxys niger bilineatus Grunberg (Diptera: Muscidae) was attracted to a wider spectrum of species, significantly attracted by four fruits and eight flowers compared with control traps, whereas S. sitiens Rondani (Diptera: Muscidae) was attracted to six fruits and seven flowers of different plants, and S. calcitrans L. (Diptera: Muscidae) was only attracted to one fruit and three flowers. Cold anthrone assays showed a significantly higher prevalence of sugar feeding amongst all three species at …

Germination And Emergence Tests For Predicting Switchgrass Field Establishment, Robert B. Mitchell, K. P. Vogel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The effect of switchgrass (Panicum virgatum L.) seed quality tests on field establishment has been addressed inadequately. Our objective was to evaluate the ability of seed quality tests to predict field establishment. Standard Association of Official Seed Analysts (AOSA) tests are based on the percentage of seeds in a seedlot that germinate under standard laboratory conditions, whereas the seedlot establishment tests (SETs) are based on the number of seeds that emerge per gram of seed using different germination or stress test conditions. The SET was determined using six laboratory tests and the results validated in three replicated field trials. …

Opportunities And Challenges From The Use Of Genomic Selection For Beef Cattle Breeding In Latin America, Hugo H. Montaldo, Eduardo Casas, José Bento Sterman Ferraz, Vicente E. Vega-Murillo, Sergio Iván Román-Ponce

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In 2009, Latin American countries had approximately 401 million cattle (29% of the world’s total cattle population) and produced 8.2 million tonnes of beef, equivalent to 29% of the world’s total production (FAO, 2011). Beef in Latin American countries is produced under widely differing climates (ranging from tropical to temperate), resources available (vegetation, food), types of markets, and genetic backgrounds of the animals. The main production systems are classified as beef and dual-purpose cattle. The genetic backgrounds of animals vary from purebred European (Bos taurus taurus) or Zebu (Bos taurus indicus) to crossbreeds (Figures 1 and …

Feasibility Of Near Infrared Spectroscopy For Analyzing Corn Kernel Damage And Viability Of Soybean And Corn Kernels, Lidia Esteve Agelet, David D. Ellis, Susan Duvick, A. J. Susana Goggi, Charles R. Hurburgh, Candice A. Gardner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The current US corn grading system accounts for the portion of damaged kernels, measured by timeconsuming and inaccurate visual inspection. Near infrared spectroscopy (NIRS), a non-destructive and fast analytical method, was tested as a tool for discriminating corn kernels with heat and frost damage. Four classification algorithms were utilized: Partial least squares discriminant analysis (PLS-DA), soft independent modeling of class analogy (SIMCA), k-nearest neighbors (K-NN), and least-squares support vector machines (LS-SVM). The feasibility of NIRS for discriminating normal or viable-germinating corn kernels and soybean seeds from abnormal or dead seeds was also tested. This application could be highly valuable for …

Water Use And Yields Of No-Till Managed Dryland Grasspea And Yellow Pea Under Different Planting Configurations, F.J. Calderón, M.F. Vigil, D.C. Nielsen, J.G. Benjamin, D.J. Poss

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Grasspea (GP) (Lathyrus sativus) is a drought-tolerant legume that can be grown for forage and grain. It has potential value to be used as a nitrogen-fixing crop in dryland rotations with non-legume grain crops. However, the agronomy of GP for the Central Great Plains region have not been investigated. The objective of this research was to compare the grain and biomass yield, as well as N accumulation of GP relative to field pea (FP) in two planting configurations. We carried out a 3-year field experiment to compare dryland GP with Admiral yellow field pea (Pisum sativum) …

Molecular Crosstalk Between A Chemical And A Biological Stressor And Consequences On Disease Manifestation In Rainbow Trout, Richard Burki, Aleksei Krasnov, Kathrin Bettge, Caird E. Rexroad Iii, Sergey Afanasyev, Miia Antikainen, Patricia Burkhardt-Holm, Thomas Wahli, Helmut Segner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The aim of the present study was to examine the molecular and organism reaction of rainbow trout, Oncorhynchus mykiss, to the combined impact of two environmental stressors. The two stressors were the myxozoan parasite, Tetracapsuloides bryosalmonae, which is the etiological agent of proliferative kidney disease (PKD) and a natural stressor to salmonid populations, and 17β-estradiol (E2) as prototype of estrogen-active chemical stressors in the aquatic environment. Both stressors, the parasite and estrogenic contaminants, co-exist in Swiss rivers and are discussed as factors contributing to the decline of Swiss brown trout populations over the last decades. Using a microarray …

The Effect Of Soil Surface Litter Residue On Energy And Carbon Fluxes In A Deciduous Forest, T.B. Wilson, T.P. Meyers, J. Kochendorfer, M. C. Anderson, M. Heuer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Atmosphere–Land Exchange Surface Energy (ALEX) balance model is an analytical formulation of the energy and mass transport within the soil and the vegetation canopy used for simulating energy, evapotranspiration, and CO2 fluxes in a wide range of vegetation environments. The objective of this study was to evaluate the ability of ALEX to simulate the effect of soil-surface leaf litter residue on soil heat conduction (G), sensible heat (H), evapotranspiration (ET) (or latent heat (LE) when expressed as rate of energy loss) and CO₂ fluxes in a deciduous forest. The model was evaluated in a deciduous forest in Oak …

An Annotated Checklist Of The Horse Flies (Diptera: Tabanidae) Of Lebanon With Remarks On Ecology And Zoogeography: Pangoniinae And Chrysopsinae, Günter Müller, Theo Zeegers, Jerome Hogsette, Edita E. Revay, Vasiliy Kravchenko, Andrey Leshvanov, Yosef Schlein

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Knowledge of the horse fly fauna (Diptera: Tabanidae) of Lebanon is fragmentary, while the local fauna of most neighboring countries has been fairly well researched. Within the framework of the 20-year project “The ecology and zoogeography of the Lepidoptera of the Near East,” we regularly collected biting flies in the whole region, including Lebanon. During this time we recorded 14 horse fly species for two subfamilies in Lebanon: four Pangoniinae and ten Chrysopsinae. Only a single species, Chrysops flavipes Meigen, 1804, was known previously in Lebanon, but the following four Pangoniinae: Pangonius haustellatus (Fabricius, 1781), Pangonius obscuratus Loew, 1859, Pangonius …

The Application Of A Visual Data Mining Framework To Determine Soil, Climate And Land-Use Relationships, Yunous Vagh

Research outputs 2012

In this research study, the methodology of action research dynamics and a case study was employed in constructing a visual data mining framework for the processing and analysis of geographic land-use data in an agricultural context. The geographic data was made up of a digital elevation model (DEM), soil and land use profiles that were juxtaposed with previously captured climatic data from fixed weather stations in Australia. In this pilot study, monthly rainfall profiles for a selected study area were used to identify areas of soil variability. The rainfall was sampled for the beginning (April) of the rainy season for …

Using An Active Optical Sensor To Improve Nitrogen Management In Corn Production, Donato Titolo

Theses and Dissertations--Plant and Soil Sciences

Corn nitrogen (N) applications are still done on a field basis in Kentucky, according to previous crop, soil tillage management and soil drainage. Soil tests, as well as plant analysis for N, are not very useful in making N fertilizer rate recommendations for corn. Recommended rates assume that only 1/3 to 2/3 of applied N is recovered, variability largely due to the strong affect of weather on the release of soil N and fertilizer N fate. Many attempts have been made to apply N in a more precise and efficient way. Two experiments were conducted at Spindeltop, the University of …

Greenhouse Production Of Strawberries During The Winter, Ellen T. Paparozzi, George E. Meyer, Stacy A. Adams, M. Elizabeth Conley, Benjamin A. Loseke, Paul E. Read

Department of Agronomy and Horticulture: Faculty Publications

Strawberries are one of America’s favorite fruits and are available in grocery stores year round. Given increased shipping and other associated costs as well as the opportunity to provide a fresh, nutritious, local product, our research team is exploring the feasibility of growing strawberries during the winter in Nebraska.

Productivity Of Field Pea (Pisumsativum L.) And Spring Oat (Avena Sativa L.) Grown As Sole And Intercrops Under Different Nitrogen Levels, Chengchou Han, Christopher Borman, Dori Osantowski, Jeremy Wagnitz, Katja Koehler-Cole, Kevin Korus, Evan Sonderegger, Rodrigo Werle, Tara Wood, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Intercropping cereal grains and legumes has potential as an alternative cool-season forage crop in low-input farming systems. The objectives of this study were to quantify the effects of density, species proportion, and nitrogen (N) supply on the biomass accumulation of field pea and spring oat grown as sole or intercrops. Greenhouse experiments were conducted using an additive series experimental design. Treatments included three fertilizer N rates and 20 density/proportion combinations in a randomized complete block with four replications. Sole plant densities included 2, 3, 4, 5 plants of each species pot-1 (16 cm in diameter, 16 cm in height). The …

Separation Of Alcohol Soluble Sorghum Proteins Using Non-Porous Cation-Exchange Columns, Deidre L. Blackwell, Scott R. Bean

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Kafirins, the storage proteins and major protein of the cereal grain sorghum, play an important nutritional role for millions of people in parts of Africa and Asia. Kafirins are non-water soluble, being soluble only in the presence of detergents or aqueous alcohol mixtures and are among the most hydrophobic of the cereal proteins. Limited M_w heterogeneity of kafirins reduces their resolution when separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Charge based separation techniques have been shown to have improved resolution of kafirins, but due to the nature of their solubility, ion-exchange (IE)-HPLC has not been widely used to …

Spillover Of Functionally Important Organisms Between Managed And Natural Habitats, Eleanor J. Blitzer, Carsten F. Dormann, Andrea Holzschuh, Alexandra-Maria Klein, Tatyana A. Rand, Teja Tscharntke

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Land-use intensification has led to a landscape mosaic that juxtaposes human-managed and natural areas. In such human-dominated and heterogeneous landscapes, spillover across habitat types, especially in systems that differ in resource availability, may be an important ecological process structuring communities. While there is much evidence for spillover from natural habitats to managed areas, little attention has been given to flow in the opposite direction. This paper synthesizes studies published to date from five functionally important trophic groups, herbivores, pathogens, pollinators, predators, and seed dispersers, and discusses evidence for spillover from managed to natural systems in all five groups. For each …

Evidence For A Major Qtl Associated With Host Response To Porcine Reproductive And Respiratory Syndrome Virus Challenge, N. Boddicker, E. H. Waide, R. R. R. Rowland, J. K. Lunney, D. J. Garrick, J. M. Reecy, J. C. M. Dekkers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Porcine reproductive and respiratory syndrome (PRRS) causes decreased reproductive performance in breeding animals and increased respiratory problems in growing animals, which result in significant economic losses in the swine industry. Vaccination has generally not been effective in the prevention of PRRS, partially because of the rapid mutation rate and evolution of the virus. The objective of the current study was to discover the genetic basis of host resistance or susceptibility to the PRRS virus through a genome-wide association study using data from the PRRS Host Genetics Consortium PRRS-CAP project.

Three groups of approximately 190 commercial crossbred pigs from 1 breeding …

Influence Of Environmental Temperature On The Physiological, Endocrine, And Immune Responses In Livestock Exposed To A Provocative Immune Challenge, J.A. Carroll, N.C. Burdick, C. C. Chase Jr, S. W. Coleman, D. E. Spiers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Although livestock experience many stressors throughout their life, one of the most commonly experienced, and most difficult to control, is stress caused by fluctuations in environmental temperatures that extend beyond the thermoneutral (TN) zone for an animal. In swine, cold stress has long been recognized as a main cause of neonatal morbidity and mortality. A possible explanation for this increased morbidity and mortality may be related to their inability to generate a febrile response. Previously, we reported that the acute phase immune response, including the generation of fever, after exposure to lipopolysaccharide (LPS; Escherichia coli O111: B4; Sigma-Aldrich, St Louis, …

Animal Foraging As A Mechanism For Sediment Movement And Soil Nutrient Development: Evidence From The Semi-Arid Australian Woodlands And The Chihuahuan Desert, David J. Eldridge, Terry B. Koen, Aaron Killgore, Niki Huang, Walter G. Whitford

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

An emerging area of interest in geomorphology over the past two decades has been the effects of biota on ecosystemprocesses.We examinedthe roles of a rangeof vertebrates onsoildisturbance in twomarkedlydifferent environments, the semi-arid woodland of eastern Australia and a Chihuahuan Desert grassland–shrubland in the south-westernUnited States. Foraging pits of soil-disturbingvertebrates variedmarkedly fromsmall scratchings of heteromyid (mainly Dipodomys spp.) rodents (1.8×10⁻⁴m³) to deep (1.0×10⁻² m³) excavations of the burrowing bettong (Bettongia leuseur) and greater bilby (Macrotis lagotis). Vertebratesmoved substantial volumes of soil in both environments, and activity was highly temporally and …

Potential Causes And Consequences Of Decreased Body Size In Field Populations Of Coccinella Novemnotata, John Losey, Jordan Perlman, James Kopco, Samuel Ramsey, Louis Hesler, Edward Evans, Leslie Allee, Rebecca Smyth

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Coccinella novemnotata, the nine-spotted lady beetle, was historically one of the most common lady beetles across the US and southern Canada. In the 1980s it became extremely rare and has remained rare. In 2008 adult C. novemnotata were collected from field populations in Oregon and South Dakota and initial observations suggested that these individuals seemed smaller than the mean size of the species historically. These observations led to a series of experiments to determine if there had been significant decrease in size and if any decrease found was due to a genetic change or to environmental factors. In the …

Transcriptome And Gene Expression Analysis In Cold-Acclimated Guayule (Parthenium Argentatum) Rubber-Producing Tissue, Grisel Ponciano, Colleen M. Mcmahan, Wenshuang Xie, Gerard R. Lazo, Terry A. Coffelt, Jillian Collins-Silva, Aise Nural-Taban, Martin Gollery, David K. Shintani, Maureen C. Whalen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Natural rubber biosynthesis in guayule (Parthenium argentatum Gray) is associated with moderately cold night temperatures. To begin to dissect the molecular events triggered by cold temperatures that govern rubber synthesis induction in guayule, the transcriptome of bark tissue, where rubber is produced, was investigated. A total of 11,748 quality expressed sequence tags (ESTs) were obtained. The vast majority of ESTs encoded proteins that are similar to stress-related proteins, whereas those encoding rubber biosynthesis- related proteins comprised just over one percent of the ESTs. Sequence information derived from the ESTs was used to design primers for quantitative analysis of the …