Agricultural Science Commons^™

Introgression Breeding For Improvement Of Winter Hardiness In Lolium /Festuca Complex Using Androgenenesis, T. Yamada, Y. D. Guo, Y. Mizukami

IGC Proceedings (1977-2023)

Intergeneric hybrids between closely related Lolium and Festuca species are used to broaden the gene pool and provide plant breeders with options to combine complementary traits to develop robust but high quality grass varieties. Androgenesis was found to be an effective procedure for selecting Lolium-Festuca genotypes comprising gene combinations rarely or never recovered by conventional backcross breeding programs. Here we describe the optimisation of androgenesis in Lolium perenne x Festuca pratensis. The male fertility and freezing tolerance of the Festulolium microspore-derived progenies were analysed and these progenies were also analysed by using genomic in situ hybridisation (GISH). The object …

A New Napier Grass Stunting Disease In Kenya Associated With Phytoplasma, A. B. Orodho, S. I. Ajanga, P. Jones, P. O. Mudavadi

IGC Proceedings (1977-2023)

Napier grass (Pennisetum purpureum Schum) is a cultivated elephant grass native to Eastern and Central Africa forming the major livestock feed on East African smallholder dairy farms (Valk, 1990) as it is suitable for cut and carry zero-grazing management systems. Although several plant pathogens have been described historically they were seldom severe. However, in the 1970s there was an outbreak of snow mould fungal disease caused by Beniowskia spheroidea that attacked most varieties of napier grass. A napier grass variety (clone 13) was bred which is resistant to the disease. In the 1990s two major outbreaks of napier grass …

Studies Of Seed Characteristics Of Ecotypes Of Lucerne, Bromus And Agropyron In Response To Fusarium Oxysporum And F. Solani, M. A. Alizadeh

IGC Proceedings (1977-2023)

Vigorous seeds and seedlings are more resistant to pathogens than non-vigorous seeds and seedlings (Kim, 1994). Therefore, it is necessary to assess seed and seedling performance in response to seed borne fungi.

Genetic Analysis Of The Interaction Between The Host Perennial Ryegrass And The Crown Rust Pathogen (Puccinia Coronata F.Sp. Lolii), P. M. Dracatos, J. L. Dumsday, R. S. Olle, N. O. I. Cogan, M. P. Dobrowolski, K. F. Smith, J. W. Forster

IGC Proceedings (1977-2023)

Crown rust (Puccinia coronata f.sp lolii) is the most important fungal pathogen of perennial ryegrass (L.perenne L.). The physiological effects associated with infection include reduction of water soluble carbohydrate (WSC) reserves, causing decreased dry matter yield, digestibility and palatability for herbivores reared for meat, milk and wool production. Phenotypic variability of rust-infection in perennial ryegrass is likely to be due to environmental effects, as well as the interaction of defence and resistance genes in the grass and virulence genes in the pathogen. Classical and molecular genetic marker-based studies have previously detected both qualitative and quantitative resistance, due respectively …

Molecular Characterisation Of Bacterial Wilt Resistance In Lolium Multiflorum Lam., B. Studer, B. Boller, F. Windmer, U. K. Posselt, E. Bauer, R. Kölliker

IGC Proceedings (1977-2023)

Italian ryegrass (Lolium multiflorum Lam.), a forage grass of prime importance throughout the world, is adversely affected by the pathogen Xanthomonas translucens pv graminis. Breeding for resistant cultivars is the only practicable means of disease control. However, the inheritance of bacterial wilt resistance is largely unknown. The aim of our research is to elucidate genetic control of bacterial wilt resistance using molecular technologies such as genetic linkage mapping and the analysis of quantitative trait loci (QTL).

Ear Emergence Of Different Grass Species Under Finnish Growing Conditions, M. Niskanen, O. Niemeläinen, L. Jauhiainen

IGC Proceedings (1977-2023)

Timothy is the most commonly cultivated grass species in Finland. Swards cultivated for silage of hay are of pure timothy or timothy is the dominant species of the mixture. Successful timing of harvesting of the primary growth is very critical in Northern latitudes where the stand develops very rapidly at daylengths of above 18 hours with a daily mean temperature close to 20 °C. The primary growth has to be harvested within a very short period to obtain a yield of high and uniform quality. Short harvesting periods require high capacity harvest machinery which lead to high costs. If it …

The Effect Of Harvest Management On Forage Production And Self-Reseeding Potential Of Italian Ryegrass (Lolium Multiflorum L.), P. W. Bartholomew, R. D. Williams

IGC Proceedings (1977-2023)

Italian ryegrass (Lolium multiflorum L.) (IRG) can be managed to produce a seed output sufficient for effective re-establishment, without compromising forage yield, it may provide an alternative to perennial cool-season grasses in the Southern Great Plains of the U.S.A. The reduction in cost of replanting and avoidance of cultivation offered by a self-seeding crop may be particularly useful in low-input production systems. We examined the effect of dates of initial harvest in spring and of partial harvests on forage yield, seed output and re-establishment of Italian ryegrass.

The Influence Of Fertiliser Application To Strip-Sown Grasslands On Herbage Production And Quality, A. Kohoutek, P. Komárek, V. Odstrčilová, P. Nerušil

IGC Proceedings (1977-2023)

To increase the productivity of dairy cattle in the Czech Republic requires an improvement in herbage quality and an increase in the net energy of herbage. The decrease in cattle numbers by 50 % and the expansion of the grassland area both result in a surplus of feedstuffs of low quality. Introduction of strip-seeding of legumes and grasses into grasslands in interaction with fertilisation provides a possible solution to this problem.

Constraints On Dairy Cattle Production From Locally Available Forages In Bangladesh, M. A. S. Khan

IGC Proceedings (1977-2023)

The productivity of milk producing animals in Bangladesh is low because of low individual yield and poor fertility. The reasons for the low productivity are complex but, in order of priority, appear to be (a) the imbalanced nature of the nutrients that arise from the digestion of the forage resources, (b) the incidence of disease/parasitism, and (c) the often harsh climatic circumstances. Thus, the purpose of this study was to find out the practical constraints on dairy cattle production from locally available forages under small holding village conditions of Bangladesh.

Pasture Management In Deer Farms In Mauritius, P. Grimaud, P. Thomas, J. Sauzier

IGC Proceedings (1977-2023)

Rusa deer (Cervus timorensis russa) were introduced into Mauritius in 1639, and with a population of 65,000 deer now provide the first source of red meat in the island. From June to September, the hunting period, extensive ranches ensure the venison market, while intensive farms provide it for the rest of the year. A pasture survey was carried out over 3 years to advise deer farmers on their sward management.

Large And Stable Genome Edits At The Sorghum Alpha Kafirin Locus Result In Changes In Chromatin Accessibility And Globally Increased Expression Of Genes Encoding Lysine Enrichment, J. Preston Hurst, Abou Yobi, Aixia Li, Shirley Sato, Thomas E. Clemente, Ruthie Angelovici

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking lysine. It has been observed that reductions in alpha-kafirin protein results in rebalancing of the seed proteome and a corresponding increase in non-kafirin proteins which leads to an increased lysine content. However, the mechanisms underlying proteome rebalancing are unclear. This study characterizes a previously developed gene edited sorghum line, with deletions at the alpha kafirin locus.

Methods: A single consensus …

Large And Stable Genome Edits At The Sorghum Alpha Kafirin Locus Result In Changes In Chromatin Accessibility And Globally Increased Expression Of Genes Encoding Lysine Enrichment, J. Preston Hurst, Abou Yobi, Aixia Li, Shirley Sato, Thomas E. Clemente, Ruthie Angelovici, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking lysine. It has been observed that reductions in alpha-kafirin protein results in rebalancing of the seed proteome and a corresponding increase in non-kafirin proteins which leads to an increased lysine content. However, the mechanisms underlying proteome rebalancing are unclear. This study characterizes a previously developed gene edited sorghum line, with deletions at the alpha kafirin locus.

Methods: A single consensus …

Discriminating Stay-Green Grasses Using Hyperspectral Imaging And Chemometrics, J. Taylor, B. Moore, J. J. Rowland, H. Thomas, H. J. Ougham

IGC Proceedings (1977-2023)

Screening of plant collections for traits can be expensive, in terms of the number of plants to be screened, the duration of the plant lifecycle and the required observations. This study describes the application of a non-invasive method, hyperspectral imaging, combined with multivariate analysis, to distinguish between homozygous wild-type (YY) Lolium multiflorum and Lolium multiflorum F2 back cross plants heterozygous for y, a recessive Festuca pratensis stay-green gene (Thomas et al., 1997).

Non-Destructive Assessment Of Quality And Yield For Grass-Breeding, A. G. T. Schut, M. J. J. Pustjens, P. W. Wilkins, J. Meuleman, P. Reyns, A. Lovatt, G. W. A. M. Van Der Heijden

IGC Proceedings (1977-2023)

Selection of cultivars has, until now, been based mainly on dry matter (DM) yields because of the high costs of sampling and chemical analysis. Imaging spectroscopy could reduce costs by limiting sampling and harvesting of individual plots to reference samples (Schut et al., accepted). In this study, the prediction accuracy of DM yields and chemical composition with imaging spectroscopy is evaluated for cultivar selection purposes.

Root Senescence In Red Clover (Trifolium Pratense L.), K. J. Webb, E. Tuck, S. Heywood

IGC Proceedings (1977-2023)

Legume root systems form a mosaic of living, ageing and dead roots and nodules. The balance between these stages alters during plant development. Stressful events (drought, temperature change, reduced carbon supply, etc.) disturb the balance (Butler et al., 1959). Effects of root and nodule death on soil structure, composition and leaching and on plant persistency are understood poorly. Plants with differing senescence patterns are useful tools to study these effects. Molecular studies of root senescence need detailed knowledge of the process and timing of root senescence and death. Biochemical and histochemical markers of senescence were used to generate preliminary …

Development Of Simple Sequence Repeat (Ssr) Markers And Their Use To Assess Genetic Diversity In Apomictic Guineagrass (Panicum Maximum Jacq.), M. Ebina, Kaori Kouki, S. Tsuruta, M. Takahara, M. Kobayashi, T. Yamamoto, K. Nakajima, H. Nakagawa

IGC Proceedings (1977-2023)

Guineagrass is an important and widely grown tropical forage grass. Despite its importance and increasing popularity, only little is known about its genetic diversity (Ebina et al., 2001). Such information is useful for the selection of diverse parents in breeding programmes. Moreover, no simple sequence repeat (SSR) markers have been reported in any apomixis species. In this study SSR markers were developed and used to investigate genetic diversity in germplasm of apomictic guineagrass.

Construction Of Microsatellite-Enriched Libraries For Tropical Forage Species And Characterization Of The Repetitive Sequences Found In Brachiaria Brizantha, Letícia Jungmann, C. B. Do Valle, P. R. Laborda, Rosangela M. S. Resende, Liana Jank, A. P. De Souza

IGC Proceedings (1977-2023)

The Brazilian cattle herd comprises 185 million animals fed with about 177 million hectares of native and cultivated pastures (IBGE, 2002). Of the grass species used for forage in Brazil, the African genus Brachiaria is the most widely planted, followed by Panicum, which also has an African origin. Legumes of the Stylosanthes genus, native to the South America, have emerged in the last few years as potential forage species for use with the grasses. These forage species have been bred at Embrapa Beef Cattle and the breeding programs have shown the need for more genetic information including the use …

Development Of Ssr Markers For Variety Identification In Italian Ryegrass (Lolium Multiflorum Lam.), M. Inoue, N. Yuyama, H. Cai

IGC Proceedings (1977-2023)

Italian ryegrass (IRG, Lolium multiflorum Lam.) is one of the most important cool-season forage grasses in the world, and is the most widely cultivated annual forage grass in Japan. Simple sequence repeat (SSR) markers have the advantages of being PCR-based, multiallelic and possessing high levels of polymorphism. They are very suitable for variety identification, especially for out-crossing species including IRG. The objective of this study was the development of SSR markers for variety identification in IRG.

Development Of A Microsatellite Library In Lolium Perenne, J. King, I. P. King, D. Thorogood, L. Roberts, K. Skøt, K. Elborough

IGC Proceedings (1977-2023)

Lolium perenne, as one of the most important forage grasses of temperate regions, combines a number of very useful characteristics, e.g., good seedling establishment, with a low resistance to drought and limited winter hardiness. Trait selection and introgression can be greatly enhanced by the use of molecular markers in a genetic linkage map. The aim of this project was the generation of a genomic microsatellite library which when combined with microsatellites developed from a Genethresher database would give good genome coverage coupled to high levels of marker polymorphism.

Isolation Of Ssr Markers From Zoysiagrass, H. Cai, N. Yuyama, M. Inoue

IGC Proceedings (1977-2023)

The genus Zoysia consists of 16 species that are naturally distributed on sea coasts and grasslands around the Pacific. In Japan, five species of natural zoysiagrasses have been identified from southern Hokkaido to the southwest islands. Of these, Z. japonica Steud. and Z. matrella Merr. have been utilized extensively as turf in Japan and other countries in East Asia. Linkage maps based on RFLP and AFLP markers have been reported in Zoysia (Yaneshita et al., 1999, Cai et al., 2004). Simple sequence repeat (SSR) markers have the advantages of being PCR-based and multiallelic. They are highly polymorphic compared …