Agricultural Science Commons^™

Overexpression Of Msdreb1c Modulates Growth And Improves Forage Quality In Tetraploid Alfalfa (Medicago Sativa L.), Yangyang Zhang, Zhen Wang, Fan Zhang, Xue Wang, Yajing Li, Ruicai Long, Mingna Li, Xianyang Li, Quanzhen Wang, Qingchuan Yang, Junmei Kang

Department of Agronomy and Horticulture: Faculty Publications

DREB has been reported to be involved in plant growth and response to environmental factors. However, the function of DREB in growth and development has not been elucidated in alfalfa (Medicago sativa L.), a perennial tetraploid forage cultivated worldwide. In this study, an ortholog of MtDREB1C was characterized from alfalfa and named MsDREB1C accordingly. MsDREB1C was significantly induced by abiotic stress. The transcription factor MsDREB1C resided in the nucleus and had self-transactivation activity. The MsDREB1C overexpression (OE) alfalfa displayed growth retardation under both long-day and short-day conditions, which was supported by decreased MsGA20ox and upregulated MsGA2ox in the OE …

Evaluating Consistency Across Multiple Neospectra (Compact Fourier Transform Near-Infrared) Spectrometers For Estimating Common Soil Properties, Sadia M. Mitu, Colleen Smith, Jonathan Sanderman, Richard R. Ferguson, Keith Shepherd, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

Rapid and cost-effective techniques for soil analysis are essential to guide sustainable land management and production agriculture. This study aimed at evaluating the performance and consistency of portable handheld Fourier-transform near-infrared spectrometers and the NeoSpectra scanners in estimating 12 common soil physical and chemical properties including pH; organic carbon (OC); inorganic carbon (IC); total nitrogen (TN); cation exchange capacity (CEC); clay, silt, and sand fractions; and exchangeable potassium (K), phosphorus (P), calcium (Ca), and magnesium (Mg). A diverse set of samples (n = 600) were retrieved from a national-scale soil archive of the Kellogg Soil Survey Laboratory of USDA-NRCS …

Arkansas Soil Erosion And Conservation Methods In Ornamental Landscapes, Abby J. Cutsinger

ATU Scholars Symposium

This study will attempt to identify the best soil erosion mitigation methods for Arkansas ornamental landscape settings by comparing the strengths and weaknesses of known techniques. Since soil is not a renewable resource, we must apply conservation practices wherever possible, not just in agricultural settings. To determine the most effective erosion prevention techniques, this study will review soil studies conducted by the NRCS and NASA, as well as articles on best practices observed in ornamental landscape and agricultural industries. These are analyzed by comparing and contrasting techniques against known problems with Arkansas soil to determine which methods are most effective. …

Accurate Measurement Of Field Size Is Essential For Analysis Of Smallholder Survey Data, Rica Joy Flor, Shen Yuan, Gonzalo Rizzo, Fahmuddin Agus, Anny Ruth P. Pame, Akhara Ouk, Rathmuny Then, Sokheng Keo, Virender Kumar, Particio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Context: Agronomic surveys play a vital role in identifying on-farm yield constraints and opportunities to increase productivity, input-use efficiency, and farmer profit. These surveys typically rely on farmer-reported data, where crop yield and applied inputs per hectare are estimated based on farmer-reported total harvested output and applied inputs per field and associated field size. At question is how accurate field size is, which is vital in the case of smallholders as small inaccuracies can lead to large biases in estimated yield and inputs.

Objectives: The goals of this study are to assess current approaches to determine field size …

Phenotypic Diversity For Morpho-Agronomic Traits In The Us Proso Millet (Panicum Miliaceum L.) Core Collection, Santosh G. Rajput, Rituraj Khound, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Proso millet (Panicum miliaceum L.) is a short-season annual crop known for high water-use efficiency and drought tolerance. The low water requirement makes this ancient grain an excellent rotational crop for the winter wheat-based dryland cropping system in theHigh Plains of theUnited States. The genetic base of the commonly grown US cultivars is very narrow. Assessment of proso millet germplasm for agronomic traits is essential for its efficient utilization in the genetic improvement of this crop. The objectives of this study were to (1) characterize the US proso millet germplasm based on nine important morpho-agronomic traits and (2) classify …

Influence Of Low-Level Condensed Tannins Concentrations In Temperate Forages On Sheep Performace, F Montossi, F Liu, J Hodgson, S T. Morris, T N. Barry, D F. Risso

IGC Proceedings (1977-2023)

Results from a series of six comparative lamb grazing trials with temperate forages are summarised, and compared with results from the literature. Condensed tannin (CT) concentrations in the diet ranged from 0.12% to 0.47% on a dry matter (DM) basis. Values were generally similar for Yorkshire fog (Holcus lanatus), perennial ryegrass (Lolium perenne) and annual ryegrass (L. multiflorum), each grown with white clover (Trifolium repens), in similar circumstances. Concentrations for tall fescue (Festuca arundinacea)/white clover pastures were relatively low, but may have been artefacts. Drenching with polyethylene glycol (PEG) to bind …

Tannin Contents Of Some Indigenous Browse Plants Of Botswana, A A. Aganga, C M. Tsopito, K M. Morake

IGC Proceedings (1977-2023)

A study was carried out to evaluate the tannin content and crude protein degradation of leaves and twigs from indigenous browsable trees which provide feed for grazing livestock on natural rangelands of Botswana. These browses include the families Leguminosae (Acacias), Capparidaceae (Boscias), Combretaceae (Combretum) and Tiliaceae (Grewias). These were found to be of good nutritive value, with the leaves having a high content of crude protein (9 - 16%) and a wide range of dry matter digestibility (37 - 69.82%). The correlation between crude protein disappearance and tannin content was found to be negative (-0.582) and significant (P<0.05).

Effect Of Tannin In Animal Diet On Nitrification Rate Of Pasture Soil Under Dung Patches, J R. Crush

IGC Proceedings (1977-2023)

The rate of formation of nitrate and ammonium in the soil under dung patches was compared for dung from animals fed either ryegrass (Lolium perenne)/white clover (Trifolium repens) or ryegrass/Lotus pedunculatus forage. Tannin derived from the Lotus did not inhibit ammonification in the soil under dung but nitrate formation was strongly inhibited. The results support the theory that polyphenolics may bind to nitrite in acid soils and cause immobilisation of mineral nitrogen. Apart from their effect of reducing the proportion of dietary nitrogen excreted in urine, tannins in animal diets may make a further contribution to …

Condensed Tannins In Tropical Legumes: Concentration, Astringency And Effects On The Nutrition Of Ruminants, R Barahona, C E. Lascano, R C. Cochran, J L. Morrill, E C. Titgemeyer

IGC Proceedings (1977-2023)

A feeding trial was carried out to determine the effect of extractable condensed tannins (ECT) concentration and tannin astringency in tropical legumes on nitrogen (N) digestion by sheep. Test legumes were Desmodium ovalifolium (Do) and Flemingia macrophylla (Fm) which had similar concentrations of Extractable CT (9% DM) but tannins with different degree of astringency (Do, 0.6 and Fm, 0.3 g protein bound/g of ECT). Chopped sun-dried forage of each legume was sprayed with either water (control) or polyethylene glycol (PEG, 3.5% DM) to reduce ECT and fed to 8 sheep with ruminal and duodenal canulas arranged in a replicated 4 …

Effect Of Condensed Tannin In Lotus Corniculatus And Lotus Pedunculatus On Digestion Of Rubisco In The Rumen, W C. Mcnabb, R J. Aerts, A Brand, J S. Peters, Y L. Foo, G C. Waghorn

IGC Proceedings (1977-2023)

The in vitro precipitation of ribulose-1,5-bisphosphate carboxylase (Rubisco) by condensed tannin (CT) extracted from Lotus corniculatus and Lotus pedunculatus and the effect of these CT on the in vitro rumen degradation of Rubisco was used to compare the reactivity of these CT. The chemical structure of CT from Lotus corniculatus was homogenous with epicatechin stereochemistry and mostly procyanidin units. The CT from Lotus pedunculatus was heterogenous with mostly prodelphinidin units. The amount of CT required to precipitate all the Rubisco when total soluble leaf protein was incubated with CT from Lotus corniculatus and Lotus pedunculatus was similar. Although CT from …

Nitrogen And Fiber Digestion In Sheep Fed Fresh-Frozen And Field-Dried High And Low Tannin Sericea Lespedeza, T H. Terrill, W R. Windham, C S. Hoveland, H E. Amos, B Kouakou, S Gelaye

IGC Proceedings (1977-2023)

Field-drying reduces extractable condensed tannin (CT) of sericea lespedeza [Lespedeza cuneata (Dum-Cours) G. Don], but effects on specific bound and unbound forms of CT and the relationship to protein and fiber digestion in ruminants are unclear. Intake and digestibility of fresh-frozen and field-dried high- and low-tannin sericea were investigated in a feeding trial with sheep (Ovis spp.). Field-drying had no effect on crude protein (CP) and fiber content of sericea, but shifted CT from extractable to bound forms. Digestion coefficients for CP, neutral detergent fiber (NDF), and acid detergent fiber (ADF) were lower for high-tannin sericea than the low-tannin …

Alteration Of Condensed Tannin Sythesis In Transgenic Forage Legumes, F Paolocci, G Tanner, S Arcioni, F Damiani

IGC Proceedings (1977-2023)

The transformation of Lotus corniculatus plants with the maize gene Sn, reorganizes the tissue specificity of condensed tannins accumulation. In particular the transformed plants show an increase of tannin content in roots and a decrease in leaves. Molecular and enzymatic analyses suggest that the transgene can functionally substitute an endogenous unknown gene not expressed in roots and induces its silencing when it is expressed. These findings could have applications for reducing tannin content in unpalatable plants and for cloning genes involved in tannin synthesis.

Comparison Of The In Vitro Fermentation Characteristic Of Fractionated Alfalfa And Sainfoin, L R. Mcmahon, M Goto, T A. Mcallister, G A. Jones, K J. Cheng

IGC Proceedings (1977-2023)

Alfalfa and sainfoin leaves were lyophilized and ground (A, S, respectively) or were fractionated into soluble (A_SOL, S_SOL) and insoluble (A_INS, S_INS)components and lyophilized and incubated in vitro with diluted ruminal fluid for 24h. Whole leaf and soluble fractions were also incubated with polyethylene glycol 8000 (PEG). Gas production (GP), ammonia concentration [NH3] and volatile fatty acid (VFA) production were lower (P< 0.05) from S and SSOL than from A and ASOL Insoluble fractions differed only in [NH3] (SINS < AINS, P < 0.05). Inclusion of PEG increased (P < 0.05) GP, [NH3] and VFA production from whole leaf and soluble fractions of sainfoin, but not alfalfa. Inactivating the condensed tannins in sainfoin with PEG overcame differences in degradability between these two forages.

Differences In Chemical Composition Among Provenances Of Tree Fodder Species In A Subhumid Environment: Relation To Use As Supplements, B H. Dzowela, L Hove, P L. Mafongoya

IGC Proceedings (1977-2023)

Studies were initiated in a subhumid Southern African environment to assess the chemical composition and nutritive value of Acacia angustissima (Mill.) Kuntze, Calliandra calothyrsus Meiss. and Leucaena species. The objective was to determine variability in crude protein (CP) concentration, acid detergent fibre (ADF), neutral detergent fibre (NDF), and soluble and insoluble proanthocyanidins from fodder samples of species, subspecies and provenances. A wide range was found in these parameters. The implications of these chemical composition factors, especially proanthocyanidins, on the use of these browse fodders in livestock feeding systems are discussed.

Nutritional Implications Of Bound Proanthocyanidins, H P.S Makkar, K Becker

IGC Proceedings (1977-2023)

The correlation between protein binding capacity of proanthocyanidin-rich neutral detergent fiber (NDF) and their proanthocyanidin (PAs) content was very low (r = -0.21; n = 6). These PAs-rich NDF did not affect growth of a tannin-sensitive strain of Clostridium perfringens. A substantial amount of bound PAs (75 to 92 %) disappeared from the NDF in 24 h of fermentation in an in vitro incubation medium containing rumen microbes. Addition of a tannin-binding agent, polyethylene glycol to the incubation medium containing rumen microbes and browses made free of extractable tannins or NDF rich in bound PAs increased the gas production up …

Environmental Effect On New Released Genotypes Of Graminaceous And Leguminous Forage Perennial Crops, P Martiniello, G Genier, R Allerit

IGC Proceedings (1977-2023)

Genotypes of perennial leguminous (alfalfa) and graminaceous (cocksfoot and tall fescue) were evaluated for adaptability in a typical Mediterranean environment (Foggia, southern Italy). The species were field evaluated to detect biomass yield potential under different types of watering. Irrigations were applied considering rainfall and FAO cultural growth coefficient for reintegrating the 75% of the evaporated water from Class A water pan. The forage harvest was applied when the shoots of the varieties reached the 10 and 50% of flowering and heading stage for leguminous and graminaceous, respectively. A split-plot experimental design, with irrigation in the main plot, was adopted. The …

Plant Tannins- Their Role In Forage Legume Quality, J A. Alokan, V A. Aletor

IGC Proceedings (1977-2023)

Forage legumes, especially browse plants, are important in the maintenance and survival of ruminant animals. However many browse species contain high levels of polyphenolic compounds including tannins. The effects of tannins on the quality of forages are reviewed. These include reduction in palatability and intake of feeds, growth rate, digestion of fibre fractions and nitrogen utilization by animals.

Genetic Manipulation Of Sainfoin Leaf Tannins, M Y. Gruber, P Auser, G Lees, A D. Muir, M Wood

IGC Proceedings (1977-2023)

An isogenic population of sainfoin (Onobrychis viciifolia Scop.) plants was developed by two methods; regeneration after long term tissue culture and plant transformation with an antisense chalcone synthase gene. Tannin (proanthocyanidin) content could be dramatically increased in untransformed callus by long term culture, but was not significantly altered in regenerating plantlets. Some of the mature transgenic plants had dramatically reduced levels of leaf tannin. The antisense gene was eliminated from all transgenic plants later in development.

Induction Of Mutants With Ectopic Expression Of Condensed Tannins, M Y. Gruber, B Skadhauge, P Auser, A D. Muir, K K. Thomsen, J Stougaard, B Coulman, D Von Wettstein

IGC Proceedings (1977-2023)

Leaves of 47,000 Lotus japonicus plants were screened using a butanol:HCl histochemical test to select “gain of function” mutants. These plants were progeny from L. japonicus lines which were transformed with T-DNA constructs containing either the maize Ds or Ac transposon (Thykjaer et al., 1995). Among 21 putative leaf tannin mutants, five (tan1-5) were characterized for synthesis of condensed tannins, leucocyanidin reductase activity and the presence of Ac and the selectable marker gene, nptII . A range of leaf tannin content among other Lotus species was also characterized.

A Revised Method Of Condensed Tannin Analysis In Leucaena Spp., S A. Dalzell, G L. Kerven

IGC Proceedings (1977-2023)

The proanthocyanidin (butanol-HCl) assay was used to measure condensed tannin (CT) in extracts from Leucaena spp. at the University of Queensland. Inconsistent results were found to be caused by the iron catalyst in the butanol/HCl reagent and the presence of ascorbic acid in the sample matrix which enhanced anthocyanidin development. In addition, preparation of sample extracts by back extraction with organic solvents reduced CT recoveries. An accurate and rapid technique was developed that measures CT directly in 70% aqueous acetone 0.1% (w/v) sodium metabisulphite plant extracts.