Conducting Polymer-Based Sensors For Food And Drug Analysis,
2021
Department of Materials Science and Engineering, National Taiwan University, Taiwan
Conducting Polymer-Based Sensors For Food And Drug Analysis, Chia-Hsin Lin, Jia-Hui Lin, Chien-Fu Chen, Yoshihiro Ito, Shyh-Chyang Luo
Journal of Food and Drug Analysis
Conducting polymers (CPs) are a category of polymeric materials with conjugated main chains. The characteristic electrical and optical properties of CPs can be fine-tuned through controlling the doping states of CPs. Because of their long-term stability in water, CPs have been demonstrated as electroactive biointerfaces and electrode materials especially in aqueous environments. Serving as multifunctional interfaces and organic electrodes for the integration bioelectronics and devices, CPs have been studied and applied in various biological applications. This paper provides a review of conducting polymer–based electrochemical sensors, particularly those used in biological fields. General conducting polymers and derivatives and their main electrochemical …
Recent Developments In Sensing Of Oversulfated Chondroitin Sulfate In Heparin. A Review .,
2021
Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan
Recent Developments In Sensing Of Oversulfated Chondroitin Sulfate In Heparin. A Review ., Manivannan Madhu, Wei-Lung Tseng
Journal of Food and Drug Analysis
Oversulfated chondroitin sulfate (OSCS), a non-natural sulfated glycosaminoglycan, recognizes as a significant containment in the pharmaceutical heparin, and it could trigger adverse reactions. Chromatography-, electrophoresis-, electrochemistry-, and spectroscopy-related techniques are currently available for accurate and precise analysis of a trace amount of OSCS in heparin. Recently, emerging studies focus on developing colorimetric and fluorescent probes to monitor OSCS containments in heparin. Therefore, this current review aims to describe the sensing principle and procedure of the reported probes that are sensitive and selective toward OSCS in heparin without the interferences of other sulfated glycosaminoglycans. The reported OSCS-specific probes are comprehensively discussed …
Comparative Toxicity, Phytochemistry, And Use Of 53 Philippine Medicinal Plants,
2021
Ateneo de Manila University
Comparative Toxicity, Phytochemistry, And Use Of 53 Philippine Medicinal Plants, Lydia M. Clemen-Pascual, Rene Angelo S. Macahig, Nina Rosario L. Rojas
Chemistry Faculty Publications
The study compares the toxicity of 53 selected medicinal plants commonly used in the Philippines to treat various diseases. It uses as a benchmark Vitex negundo L., which was approved by the Philippine Food and Drug Administration as an herbal drug for cough and asthma after passing clinical trials for safety and efficacy. The methods were chosen for their simplicity and accessibility even for resource-limited laboratories. Extracts (95 % ethanol) of the medicinal parts of the plants were (1) chemically profiled using qualitative phytochemical tests that detect the presence of key classes of bioactive compounds; and (2) evaluated for toxicity …
Translating Particulate Hexavalent Chomium-Induced Chromosome Instability, Loss Of Homologous Recombination Repair And Targeting Of Rad51 From Human Lung Fibroblasts To Human Bronchial Epithelial Cells.,
2021
University of Louisville
Translating Particulate Hexavalent Chomium-Induced Chromosome Instability, Loss Of Homologous Recombination Repair And Targeting Of Rad51 From Human Lung Fibroblasts To Human Bronchial Epithelial Cells., Idoia Meaza Isusi
Electronic Theses and Dissertations
Particulate hexavalent chromium [Cr(VI)] is a well-established human lung carcinogen. RAD51, a key protein in homologous recombination repair pathway, is inhibited after prolonged exposure to Cr(VI), leading to an increase in chromosome instability after prolonged exposures in human lung fibroblasts. chromosome instability is the proposed driver of Cr(VI) carcinogenesis. Since tumors from chromate workers develop from epithelial cells, we sought to translate these findings from human bronchial fibroblasts to human bronchial epithelial cells. We hypothesized Cr(VI) inhibits RAD51 after prolonged exposure leading to an increase in chromosome instability in human bronchial epithelial cells (BEP2D). We characterized the cytotoxicity and measured …
Toxicity And Effects Of Tire Crumb Rubber In The Aquatic Environment,
2021
Clemson University
Toxicity And Effects Of Tire Crumb Rubber In The Aquatic Environment, Stephanie Laplaca
All Dissertations
Plastic materials have provided innovative solutions to society’s evolving needs and challenges. Due to their durability and resistance to degradation, plastics remain in the environment for long periods of time and can therefore be transported to many environmental compartments such as water, sediment, and biota. Microplastics (MPs) have been defined as synthetic plastic particles that have at least one dimension less than 5 mm and are insoluble in water. Microrubber (MR), a recently acknowledged sub-group of MPs, has been documented in environmental samples more recently, frequently comprising a large portion of total MPs of various samples. In South Carolina specifically, …
Characterizing The Role Of Macrophages In Cisplatin-Induced Kidney Injury And Progression To Chronic Kidney Disease.,
2021
University of Louisville
Characterizing The Role Of Macrophages In Cisplatin-Induced Kidney Injury And Progression To Chronic Kidney Disease., Sophia M. Sears
Electronic Theses and Dissertations
Cisplatin is a commonly used chemotherapeutic for treatment of many solid-organ cancers. Unfortunately, 30% of patients treated with cisplatin develop acute kidney injury (AKI), and even patients who do not develop AKI are at risk for long term declines in kidney function and development of chronic kidney disease (CKD). While traditional rodent toxicity studies have utilized a single, lethal dose of cisplatin, new models of cisplatin-induced kidney injury have revealed that repeated, low doses of cisplatin lead to development of kidney fibrosis. This model can be used to examine AKI-to-CKD transition processes. C57BL/6 mice are one of the most used …
Development And Validation Of A Method For The Determination Of Designer Benzodiazepines In Hair By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms),
2021
CUNY John Jay College
Development And Validation Of A Method For The Determination Of Designer Benzodiazepines In Hair By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Laura C. Defreitas
Student Theses
In recent years, new designer benzodiazepines have become a challenge in forensic toxicology. These substances are analogues of the classic benzodiazepines, but their pharmacology is not well known, and many of them have been associated with overdoses and deaths. As a result, there has been a surge in efforts to develop ways to accurately test for these compounds in different biological matrices. This study focused to develop and validate a method for determining 17 new designer benzodiazepines in hair by liquid chromatography tandem mass spectrometry (LC-MS/MS). Hair samples were decontaminated, pulverized, and 20 mg of the sample was incubated in …
Investigation Of Toxic Chemicals In Plant-Based Milk Alternatives,
2021
Texas Southern University
Investigation Of Toxic Chemicals In Plant-Based Milk Alternatives, Chukwunonso A. Anakwue
Dissertations (2016-Present)
Milk is a dietary component consumed all over the world and is known to be essential for development in children. Despite all the nutritional advantages associated with milk consumption, several issues like lactose intolerance, and high lipid content, etc., negatively affects the perception of cow milk consumption, and has grown into general health concerns. Issues with cow milk consumption drove for a healthier alternative and this led to plant-based milk alternatives (PBMA). Several studies have linked consumption of these PBMAs with other negative effects such as: allergy, poor nutrient profile and the presence of other foreign chemicals. This study’s main …
A Study On The Efficacy Of A Naloxone Training Program,
2021
Lehigh Valley Health Network
A Study On The Efficacy Of A Naloxone Training Program, Gillian A. Beauchamp Md, Hoonani M. Cuadrado, Seth A. Campbell Pa-C, Bennie Eliason Chw, Chase Jones Do, Aaron Fedor Do, Lauren Grantz Pharmd, Csp, Paige Roth Lsw, Marna Greenberg
Department of Emergency Medicine
Introduction: The use of naloxone to reverse a potentially fatal opioid overdose is a harm reduction strategy that reduces mortality and increases the potential for referral to substance use treatment for affected individuals. In the setting of outreach performed by a street medicine team, we aimed to determine the effectiveness of an educational intervention involving distribution of naloxone accompanied by a brief instructive session about opioids, opioid overdose, and medication administration. Methods: Our street medicine outreach team distributed 200 naloxone kits to clinicians and volunteers involved in caring for patients on ‘street rounds,’ as well as in shelters, soup kitchens, …
Characterization Of A Fluorescent Glucose Derivative 1-Nbdg And Its Application In The Identification Of Natural Sglt1/2 Inhibitors,
2021
School of Pharmacy, National Taiwan University, Taipei, Taiwan
Characterization Of A Fluorescent Glucose Derivative 1-Nbdg And Its Application In The Identification Of Natural Sglt1/2 Inhibitors, Ting-Yu Kao, Hwa-Wei Wu, Shoei-Sheng Lee, Pi-Hui Liang, Jih-Hwa Guh, Lih-Ching Hsu
Journal of Food and Drug Analysis
Glucose is an important energy source for cells. Glucose transport is mediated by two types of glucose transporters: the active sodium-coupled glucose cotransporters (SGLTs), and the passive glucose transporters (GLUTs). Development of an easy way to detect glucose uptake by the cell can be valuable for research. 1-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-1-deoxy-D-glucose (1-NBDG) is a newly synthesized fluorescent glucose analogue. Unlike 2-NBDG, which is a good substrate of GLUTs but not SGLTs, 1-NBDG can be transported by both GLUTs and SGLTs. Thus, 1-NBDG is useful for the screening of SGLT1 and SGLT2 inhibitors. Here we further characterized 1-NBDG and compared it …
Lucigenin-Pyrogallol Chemiluminescence For The Multiple Detection Of Pyrogallol, Cobalt Ion, And Tyrosinase,
2021
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun, Jilin, PR China
Lucigenin-Pyrogallol Chemiluminescence For The Multiple Detection Of Pyrogallol, Cobalt Ion, And Tyrosinase, Islam M. Mostafa, Muhammad Rehan Hasan Shah Gilani, Yequan Chen, Baohua Lou, Jianping Li, Guobao Xu
Journal of Food and Drug Analysis
Developing new, cheap, sensitive and selective chemiluminescence (CL) systems with multiple detection properties is still a big challenge for biological and environmental applications. Here, we report a new CL system having multiple detection applications (environmental and biological). The developed lucigenin-pyrogallol system gave an enhancement (190 times) over the conventional lucigenin-H2O2 CL system. Cobalt ion and tyrosinase can inhibit lucigenin-PG CL intensity. Based on these phenomena, we have developed new CL methods for the detection of pyrogallol (LOD = 0.94 μM), lucigenin (LOD = 0.42 μM), cobalt (LOD = 68 μM), and tyrosinase (LOD = 1.8 μg/mL). Furthermore, …
Determination Of Cannabinoids In Hemp Oil Based Cosmetic Products By Lc-Tandem Ms,
2021
Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan
Determination Of Cannabinoids In Hemp Oil Based Cosmetic Products By Lc-Tandem Ms, Yun-Hua Hsu, Ming-Chih Fang, Shou-Chieh Huang, Ya-Min Kao, Su-Hsiang Tseng, Der-Yuan Wang
Journal of Food and Drug Analysis
Cosmetic products containing hemp seed oil as permitted raw materials required the specific compound delta-9-tetrahydrocannabinol (THC) below 10 µg/g. THC was the main psychoactive constituent of cannabis. Since hemp seed oil became an increasingly popular ingredient in cosmetics over the last few years, an efficient and reliable analytical method for THC and other cannabinoids in cannabis-infused cosmetic products was in need. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of delta-9-tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabinol (CBN) in hemp seed oil based cosmetic products was developed. Method validation was performed by fertilizing blank samples with analytes and internal …
Comprehensive Multiplexed Analysis Of Risky Drugs In Eggs Based On Magnetic Zeolitic Imidazolate Frameworks And Uhplc Q-Orbitrap Hrms,
2021
Shaanxi University of Science and Technology, China
Comprehensive Multiplexed Analysis Of Risky Drugs In Eggs Based On Magnetic Zeolitic Imidazolate Frameworks And Uhplc Q-Orbitrap Hrms, Wei Jia, Yuyang Liu, Xi Xu, Yanxi Zhang, Lin Shi
Journal of Food and Drug Analysis
A novel magnetic adsorbent solid-phase Fe3O4@ZIF-8 extraction method coupled with UHPLC Q-Orbitrap high resolution mass spectrometry for the sensitive and high efficiency detection of fluoroquinolones, tetracyclines, dyes and amphenicols in eggs is described. Under optimum conditions, CCβ and CCα of 0.62 to 26.97 μg.kg–1 and 0.36 to 16.37 μg.kg–1 was obtained, which is much better than that obtained by conventional methods. The accuracy, expressed as recovery, were achieved falling between 75% and 104%. The proposed strategy provides several advantages of simplicity and low energy consumption, thus retaining potential for the screening of …
An Extractive Nanoelectrospray Ionization-Mass Spectrometry Method For Chinese Herbal Medicine Authentication,
2021
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
An Extractive Nanoelectrospray Ionization-Mass Spectrometry Method For Chinese Herbal Medicine Authentication, Chia-Hsin Lee, Hui-Chi Huang, Mei-Chun Tseng, Chao-Jung Chen
Journal of Food and Drug Analysis
Herein, we describe a rapid, sensitive, and nondestructive method—extractive nanoelectrospray ionization-mass spectrometry (EnESI-MS)—for traditional Chinese medicine (TCM) authentication. The mass-spectral fingerprints of volatile compounds released from various TCMs can be rapidly acquired using EnESI-MS without sample pretreatment. EnESI-MS was applied to successfully differentiate between two commonly used medicinal herbs, Schisandra Chinensis and Schisandra sphenanthera, which are morphologically similar but exhibit different therapeutic effects. Specific volatile compounds of each herb in a ten-component Chinese herbal product, Jia Wei Xiao Yao San, were also identified, and the method was applied to discriminate between the commercial product and a substandard version.
Mesonosides A-H, Primeverose Derivatives From Mesona Procumbens Suppress Adipogenesis By Downregulating Pparγ And C/Ebpa In 3t3-L1 Cells,
2021
Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei, Taiwan
Mesonosides A-H, Primeverose Derivatives From Mesona Procumbens Suppress Adipogenesis By Downregulating Pparγ And C/Ebpa In 3t3-L1 Cells, Hung-Tse Huang, Chia-Ching Liaw, Chun-Tang Chiou, Kung-Ta Lee, Yao-Haur Kuo
Journal of Food and Drug Analysis
Obesity is becoming a worldwide epidemic, especially in industrialized countries. We hereby report a methanolic extract of Mesona procumbens (known as Hsian-tsao in Taiwan) significantly inhibits lipid accumulation in 3T3-L1 adipocytes, and eight new primeverose derivatives, mesonosides A-H (1-8), were isolated from the methanolic extract of M. procumbens. Structural elucidation of 1-8 was established by spectroscopic methods, especially 2D NMR techniques (1H-1H COSY, HSQC, HMBC, and NOESY) and HRESIMS. Anti-obesity evaluation revealed that isolates 1-5, 7, and 8 showed inhibitory effects on lipid accumulation and protein levels of adipogenic transcription factor, PPARγ and C/EBPa in 3T3-L1 …
Induction Of Mitochondrial-Dependent Apoptosis By Essential Oil Of Toona Sinensis Root Through Akt, Mtor And Nf-Κb Signalling Pathways In Human Renal Cell Carcinoma Cells,
2021
Department of Urology, E-Da Cancer Hospital, Kaohsiung, Taiwan
Induction Of Mitochondrial-Dependent Apoptosis By Essential Oil Of Toona Sinensis Root Through Akt, Mtor And Nf-Κb Signalling Pathways In Human Renal Cell Carcinoma Cells, Yu-Chi Chen, Chiao-Ling Hsieh, Bu-Miin Huang, Yungchia Chen
Journal of Food and Drug Analysis
Natural products have long been considered as a kind of complementary medicine. In this study, we investigate the apoptotic effect of essential oils of Toona sinensis roots (TSR) on human clear cell renal cell carcinomas (ccRCC). The sesquiterpene content of TSR essential oil was determined via GC/MS analysis. TSR decreased ccRCC cell viabilities, inducing ROS generation and reduction of the mitochondrial membrane potential. Moreover, TSR inhibited Bcl-2 and Hsp90 expression but increased PARP-1 cleavage and cytochrome c release. Akt, mTOR and NF-kB phosphorylation and HIF-a expression were all inhibited, which likely contributed to the anti-proliferative and anti-adhesive effects of TSR.
Comprehensive Detection Of 120 Additives In Food Using Nontargeted Ms Data Acquisition,
2021
Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan
Comprehensive Detection Of 120 Additives In Food Using Nontargeted Ms Data Acquisition, Che-Lun Hsu, Mingchih Fang, Hui-Chun Tseng, Ya-Tze Lin, Su-Hsiang Tseng, Der-Yuan Wang, Deng-Fwu Hwang
Journal of Food and Drug Analysis
The compliance assessment on the labeling of food additives is a hard job, because there are nearly thousand legal food additives can be used in food, and countless illegal additives must also deal with. This study developed a nontargeted data acquisition screening method based on liquid chromatography high resolution mass spectrometry (HRMS) in which a precursor ion and two product ions of each analyte are able to be recorded. The high throughput screening method worked as foodomics that characterized and identified every food components as long as they were ionized in terms of theory. The data acquisition method called data …
Transcriptome Analysis Of Lactobacillus Rhamnosus Gg Strain Treated With Prebiotic - Bovine Lactoferrin Under A Cold Environment,
2021
Department of Safety, Health and Environmental Engineering, Ming Chi University of Technology, New Taipei City, Taiwan
Transcriptome Analysis Of Lactobacillus Rhamnosus Gg Strain Treated With Prebiotic - Bovine Lactoferrin Under A Cold Environment, Zhen-Shu Liu, Chuen-Fu Lin, Po-Wen Chen
Journal of Food and Drug Analysis
Lactoferrin (LF) is secreted by mammals and displays extensive biological effects. We previously reported that bovine LF (BLF) can boost the cold tolerance of a well-applied probiotic strain, Lactobacillus rhamnosus GG (LGG), to grow robustly under a cold environment, but the molecular mechanism is not clear. Here, RNA-seq analysis was conducted to ascertain molecular pathways underlying cold tolerance exerted by BLF. LGG was cultured in a cold environment (22°C) in the presence or absence of BLF. Transcriptome analysis indicated that BLF significantly elicited 1.2 to 3.2 fold (log2 Fold change) higher expression levels of genes related to stress, defense, cell …
Polydopamine And Silica Nanoparticles Magnetic Solid Phase Extraction Coupled With Liquid Chromatography-Tandem Mass Spectrometry To Determine Phenolic Acids And Flavonoids In Fruit Wine,
2021
Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan
Polydopamine And Silica Nanoparticles Magnetic Solid Phase Extraction Coupled With Liquid Chromatography-Tandem Mass Spectrometry To Determine Phenolic Acids And Flavonoids In Fruit Wine, Hui-Ling Lee, Chih-Yuan Kang, Yen-Jung Kuo, Shan-Ni Tseng
Journal of Food and Drug Analysis
Magnetic solid phase extraction (MSPE) have been widely applied in a variety of sample preparation techniques. Herein, Fe3O4@pDA as the sorbents for MSPE, were developed for the determination of phenolic acids and flavonoids in fruit wine samples in combination with LC-MS/MS. The Fe3O4@pDA were characterized by Fourier transform infrared spectroscopy (FT-IR), powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), Superconducting Quantum Interference Device Magnetometer (SQUID) and thermogravimetric analysis (TGA) in detail. In the present study, a new, rapid, and efficient MSPE by LC-MS/MS was established for the extraction and sensitive detection of phenolic acids and flavonoids. Under the optimized condition …
Microcystis Sp. Co-Producing Microcystin And Saxitoxin From Songkhla Lake Basin, Thailand,
2021
Prince of Songkla University
Microcystis Sp. Co-Producing Microcystin And Saxitoxin From Songkhla Lake Basin, Thailand, Ampapan Naknaen, Waraporn Ratsameepakai, Oramas Suttinun, Yaowapa Sukpondma, Eakalak Khan, Rattanaruji Pomwised
Civil and Environmental Engineering and Construction Faculty Research
The Songkhla Lake Basin (SLB) located in Southern Thailand, has been increasingly polluted by urban and industrial wastewater, while the lake water has been intensively used. Here, we aimed to investigate cyanobacteria and cyanotoxins in the SLB. Ten cyanobacteria isolates were identified as Microcystis genus based on16S rDNA analysis. All isolates harbored microcystin genes, while five of them carried saxitoxin genes. On day 15 of culturing, the specific growth rate and Chl-a content were 0.2–0.3 per day and 4 µg/mL. The total extracellular polymeric substances (EPS) content was 0.37–0.49 µg/mL. The concentration of soluble EPS (sEPS) was 2 times higher …
