Comprehensive Multiplexed Analysis Of Risky Drugs In Eggs Based On Magnetic Zeolitic Imidazolate Frameworks And Uhplc Q-Orbitrap Hrms,
2021
Shaanxi University of Science and Technology, China
Comprehensive Multiplexed Analysis Of Risky Drugs In Eggs Based On Magnetic Zeolitic Imidazolate Frameworks And Uhplc Q-Orbitrap Hrms, Wei Jia, Yuyang Liu, Xi Xu, Yanxi Zhang, Lin Shi
Journal of Food and Drug Analysis
A novel magnetic adsorbent solid-phase Fe3O4@ZIF-8 extraction method coupled with UHPLC Q-Orbitrap high resolution mass spectrometry for the sensitive and high efficiency detection of fluoroquinolones, tetracyclines, dyes and amphenicols in eggs is described. Under optimum conditions, CCβ and CCα of 0.62 to 26.97 μg.kg–1 and 0.36 to 16.37 μg.kg–1 was obtained, which is much better than that obtained by conventional methods. The accuracy, expressed as recovery, were achieved falling between 75% and 104%. The proposed strategy provides several advantages of simplicity and low energy consumption, thus retaining potential for the screening of …
An Extractive Nanoelectrospray Ionization-Mass Spectrometry Method For Chinese Herbal Medicine Authentication,
2021
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
An Extractive Nanoelectrospray Ionization-Mass Spectrometry Method For Chinese Herbal Medicine Authentication, Chia-Hsin Lee, Hui-Chi Huang, Mei-Chun Tseng, Chao-Jung Chen
Journal of Food and Drug Analysis
Herein, we describe a rapid, sensitive, and nondestructive method—extractive nanoelectrospray ionization-mass spectrometry (EnESI-MS)—for traditional Chinese medicine (TCM) authentication. The mass-spectral fingerprints of volatile compounds released from various TCMs can be rapidly acquired using EnESI-MS without sample pretreatment. EnESI-MS was applied to successfully differentiate between two commonly used medicinal herbs, Schisandra Chinensis and Schisandra sphenanthera, which are morphologically similar but exhibit different therapeutic effects. Specific volatile compounds of each herb in a ten-component Chinese herbal product, Jia Wei Xiao Yao San, were also identified, and the method was applied to discriminate between the commercial product and a substandard version.
Mesonosides A-H, Primeverose Derivatives From Mesona Procumbens Suppress Adipogenesis By Downregulating Pparγ And C/Ebpa In 3t3-L1 Cells,
2021
Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei, Taiwan
Mesonosides A-H, Primeverose Derivatives From Mesona Procumbens Suppress Adipogenesis By Downregulating Pparγ And C/Ebpa In 3t3-L1 Cells, Hung-Tse Huang, Chia-Ching Liaw, Chun-Tang Chiou, Kung-Ta Lee, Yao-Haur Kuo
Journal of Food and Drug Analysis
Obesity is becoming a worldwide epidemic, especially in industrialized countries. We hereby report a methanolic extract of Mesona procumbens (known as Hsian-tsao in Taiwan) significantly inhibits lipid accumulation in 3T3-L1 adipocytes, and eight new primeverose derivatives, mesonosides A-H (1-8), were isolated from the methanolic extract of M. procumbens. Structural elucidation of 1-8 was established by spectroscopic methods, especially 2D NMR techniques (1H-1H COSY, HSQC, HMBC, and NOESY) and HRESIMS. Anti-obesity evaluation revealed that isolates 1-5, 7, and 8 showed inhibitory effects on lipid accumulation and protein levels of adipogenic transcription factor, PPARγ and C/EBPa in 3T3-L1 …
Induction Of Mitochondrial-Dependent Apoptosis By Essential Oil Of Toona Sinensis Root Through Akt, Mtor And Nf-Κb Signalling Pathways In Human Renal Cell Carcinoma Cells,
2021
Department of Urology, E-Da Cancer Hospital, Kaohsiung, Taiwan
Induction Of Mitochondrial-Dependent Apoptosis By Essential Oil Of Toona Sinensis Root Through Akt, Mtor And Nf-Κb Signalling Pathways In Human Renal Cell Carcinoma Cells, Yu-Chi Chen, Chiao-Ling Hsieh, Bu-Miin Huang, Yungchia Chen
Journal of Food and Drug Analysis
Natural products have long been considered as a kind of complementary medicine. In this study, we investigate the apoptotic effect of essential oils of Toona sinensis roots (TSR) on human clear cell renal cell carcinomas (ccRCC). The sesquiterpene content of TSR essential oil was determined via GC/MS analysis. TSR decreased ccRCC cell viabilities, inducing ROS generation and reduction of the mitochondrial membrane potential. Moreover, TSR inhibited Bcl-2 and Hsp90 expression but increased PARP-1 cleavage and cytochrome c release. Akt, mTOR and NF-kB phosphorylation and HIF-a expression were all inhibited, which likely contributed to the anti-proliferative and anti-adhesive effects of TSR.
Comprehensive Detection Of 120 Additives In Food Using Nontargeted Ms Data Acquisition,
2021
Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan
Comprehensive Detection Of 120 Additives In Food Using Nontargeted Ms Data Acquisition, Che-Lun Hsu, Mingchih Fang, Hui-Chun Tseng, Ya-Tze Lin, Su-Hsiang Tseng, Der-Yuan Wang, Deng-Fwu Hwang
Journal of Food and Drug Analysis
The compliance assessment on the labeling of food additives is a hard job, because there are nearly thousand legal food additives can be used in food, and countless illegal additives must also deal with. This study developed a nontargeted data acquisition screening method based on liquid chromatography high resolution mass spectrometry (HRMS) in which a precursor ion and two product ions of each analyte are able to be recorded. The high throughput screening method worked as foodomics that characterized and identified every food components as long as they were ionized in terms of theory. The data acquisition method called data …
Transcriptome Analysis Of Lactobacillus Rhamnosus Gg Strain Treated With Prebiotic - Bovine Lactoferrin Under A Cold Environment,
2021
Department of Safety, Health and Environmental Engineering, Ming Chi University of Technology, New Taipei City, Taiwan
Transcriptome Analysis Of Lactobacillus Rhamnosus Gg Strain Treated With Prebiotic - Bovine Lactoferrin Under A Cold Environment, Zhen-Shu Liu, Chuen-Fu Lin, Po-Wen Chen
Journal of Food and Drug Analysis
Lactoferrin (LF) is secreted by mammals and displays extensive biological effects. We previously reported that bovine LF (BLF) can boost the cold tolerance of a well-applied probiotic strain, Lactobacillus rhamnosus GG (LGG), to grow robustly under a cold environment, but the molecular mechanism is not clear. Here, RNA-seq analysis was conducted to ascertain molecular pathways underlying cold tolerance exerted by BLF. LGG was cultured in a cold environment (22°C) in the presence or absence of BLF. Transcriptome analysis indicated that BLF significantly elicited 1.2 to 3.2 fold (log2 Fold change) higher expression levels of genes related to stress, defense, cell …
Polydopamine And Silica Nanoparticles Magnetic Solid Phase Extraction Coupled With Liquid Chromatography-Tandem Mass Spectrometry To Determine Phenolic Acids And Flavonoids In Fruit Wine,
2021
Department of Chemistry, Fu Jen Catholic University, New Taipei City, Taiwan
Polydopamine And Silica Nanoparticles Magnetic Solid Phase Extraction Coupled With Liquid Chromatography-Tandem Mass Spectrometry To Determine Phenolic Acids And Flavonoids In Fruit Wine, Hui-Ling Lee, Chih-Yuan Kang, Yen-Jung Kuo, Shan-Ni Tseng
Journal of Food and Drug Analysis
Magnetic solid phase extraction (MSPE) have been widely applied in a variety of sample preparation techniques. Herein, Fe3O4@pDA as the sorbents for MSPE, were developed for the determination of phenolic acids and flavonoids in fruit wine samples in combination with LC-MS/MS. The Fe3O4@pDA were characterized by Fourier transform infrared spectroscopy (FT-IR), powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), Superconducting Quantum Interference Device Magnetometer (SQUID) and thermogravimetric analysis (TGA) in detail. In the present study, a new, rapid, and efficient MSPE by LC-MS/MS was established for the extraction and sensitive detection of phenolic acids and flavonoids. Under the optimized condition …
Microcystis Sp. Co-Producing Microcystin And Saxitoxin From Songkhla Lake Basin, Thailand,
2021
Prince of Songkla University
Microcystis Sp. Co-Producing Microcystin And Saxitoxin From Songkhla Lake Basin, Thailand, Ampapan Naknaen, Waraporn Ratsameepakai, Oramas Suttinun, Yaowapa Sukpondma, Eakalak Khan, Rattanaruji Pomwised
Civil and Environmental Engineering and Construction Faculty Research
The Songkhla Lake Basin (SLB) located in Southern Thailand, has been increasingly polluted by urban and industrial wastewater, while the lake water has been intensively used. Here, we aimed to investigate cyanobacteria and cyanotoxins in the SLB. Ten cyanobacteria isolates were identified as Microcystis genus based on16S rDNA analysis. All isolates harbored microcystin genes, while five of them carried saxitoxin genes. On day 15 of culturing, the specific growth rate and Chl-a content were 0.2–0.3 per day and 4 µg/mL. The total extracellular polymeric substances (EPS) content was 0.37–0.49 µg/mL. The concentration of soluble EPS (sEPS) was 2 times higher …
Impact Of Sodium Dichloroacetate Alone And In Combination Therapies On Lung Tumor Growth And Metastasis,
2021
United Arab Emirates University
Impact Of Sodium Dichloroacetate Alone And In Combination Therapies On Lung Tumor Growth And Metastasis, Aya Mudhafar A. Al-Azawi
Theses
Lung cancer is the second most common form of cancer with the highest mortality rate worldwide in 2020 despite the advances in targeted- and immuno-therapies. Metabolic reprogramming has been recognized as an essential emerging cancer hallmark in which altered metabolic pathways represent an attractive therapeutic target. Sodium Dichloroacetate (DCA), a pyruvate dehydrogenase kinase (PDK) inhibitor, effect has been investigated in various tumors. Building on the already published data, this pre-clinical study aims to explore the anticancer potential of DCA in lung cancer alone and in combination with chemo- and targeted therapies using two non-small cell lung cancer (NSCLC) cell lines …
Do Environmental Toxins Predict Violent Crimes?,
2021
Kennesaw State University
Do Environmental Toxins Predict Violent Crimes?, Tyler Stahl
Symposium of Student Scholars
Do chemical pollutants that persistent in the environment and bioaccumulate in the body affect human health and behavior? Could these Persistent, Bioaccumulative, and Toxic (PBT) chemicals play a role in the cause of violent crimes due to deterioration of mental and cognitive functions? In the past, Mercury, a PBT chemical, has been shown in salmon to be associated with aggression. Could similar aggression occur in humans exposed to mercury through a toxic spill? Two sources of data are utilized in this analysis. The Environmental Protection Agency’s (EPA) Annual Toxic Release Inventory publishes data on toxic releases into the environment and …
In Vivo Antidiabetic Activities Of Polyherbal Extracts Against Streptozotocin-Nicotinamide Induced Type 2 Diabetic Mice Model,
2021
PDEA’S Seth Govind Raghunath Sable College of Pharmacy, Saswad, Maharashtra, India
In Vivo Antidiabetic Activities Of Polyherbal Extracts Against Streptozotocin-Nicotinamide Induced Type 2 Diabetic Mice Model, Pradnya Jagtap, Yashashri Nimbalkar, Vidya Patil, Ravindra Y. Patil
Tanzania Journal of Science
Diabetes mellitus is a chronic metabolic disorder and rapidly increasing cases of diabetes throughout the world are major concerns in both developed and developing countries. Currently available oral antidiabetic medicines have limitations in efficacy and safety, which in conjunction with the emergence of diabetes mellitus as a global epidemic have aided the popularity of alternative therapies to manage the disease safely and more efficiently. Herbal medicines are accepted as alternative therapies for blood sugar control. Gymnema sylvestre, Boerhavia diffusa, Tinospora cordifolia, and Argyreia nervosa are some potent herbs used for the management of diabetes. Mixing of these plants in different …
Prunus Africanus Herbal Extracts Reverse And Ameliorate The Histological And Histomorphometric Changes In Testosterone-Induced Benign Prostate Hyperplasia Rat Models,
2021
Department of Anatomy and Histology, School of Medicine, Muhimbili University of Health and Allied Sciences, P. O. Box 65001, Dar es Salaam, Tanzania.
Prunus Africanus Herbal Extracts Reverse And Ameliorate The Histological And Histomorphometric Changes In Testosterone-Induced Benign Prostate Hyperplasia Rat Models, Felix P. William, Denis A. Russa
Tanzania Journal of Science
Benign prostatic hyperplasia (BPH) is the most common benign proliferative disease among men during aging. The herbal extract of P. africanus has been used for the treatment of BPH since time immemorial. However microstructural changes of this extract to the prostate of animal or human models are still elusive. Therefore, histological and histomorphometric changes in rats with testosterone-induced BPH due to P. africanus herbal extracts were investigated. Twenty-eight male Wistar rats (200 ± 50 g) were divided into four groups each with 7 rats. Group 1 (Negative control) was given 2 ml/day of olive oil subcutaneously and 2 ml/day of …
Childhood Exposure To Per- And Polyfluoroalkyl Substances (Pfas) And Neurobehavioral Domains In Children At Age 8 Years,
2021
University of Nevada, Las Vegas
Childhood Exposure To Per- And Polyfluoroalkyl Substances (Pfas) And Neurobehavioral Domains In Children At Age 8 Years, Ann M. Vuong, Kimberly Yolton, Changchun Xie, Kim N. Dietrich, Joseph M. Braun, Glenys M. Webster, Antonia M. Calafat, Bruce P. Lanphear, Aimin Chen
Environmental & Global Health Faculty Research
Background: Toxicological studies have raised concerns regarding the neurotoxic effects of per- and polyfluoroalkyl substances (PFAS). However, observational evidence from human studies investigating the association between childhood PFAS and neurobehavior is limited and remains unclear. Objectives: To examine whether childhood PFAS concentrations are associated with neurobehavior in children at age 8 years and whether child sex modifies this relationship. Methods: We used data from 208 mother-child dyads in the Health Outcomes and Measures of the Environment (HOME) Study, a prospective pregnancy and birth cohort (Cincinnati, OH, USA). We quantified PFAS in child serum at 3 and 8 years. We assessed …
Cocaine, Cocaethylene And Ethanol In Postmortem Blood Submitted To The New York City Office Of Chief Medical Examiner,
2021
CUNY John Jay College
Cocaine, Cocaethylene And Ethanol In Postmortem Blood Submitted To The New York City Office Of Chief Medical Examiner, Jada T. Alexis
Student Theses
Cocaine overdose deaths are on the rise in the United States. This increase may be due to the co-consumption of cocaine with other drugs. When cocaine is ingested in conjunction with alcohol, a specific metabolite called cocaethylene forms. Information regarding cocaethylene, specifically its interpretive value in post-mortem cases, has not been widely studied. Data from the Office of the Chief Medical Examiner in New York City revealed that there were 195 cases in 2017 positive for cocaethylene. These cases were most likely to involve black (42.1%) males (79.5%) in Manhattan (28.2%) aged 50 - 59 (34.4%). Most of the cases …
Innate Immune Activation By Checkpoint Inhibition In Human Patient-Derived Lung Cancer Tissues,
2021
University of Kentucky
Innate Immune Activation By Checkpoint Inhibition In Human Patient-Derived Lung Cancer Tissues, Teresa W. M. Fan, Richard M. Higashi, Huan Song, Saeed Daneshmandi, Angela L. Mahan, Matthew S. Purdom, Therese J. Bocklage, Thomas A. Pittman, Daheng He, Chi Wang, Andrew N. Lane
Center for Environmental and Systems Biochemistry Faculty Publications
Although Pembrolizumab-based immunotherapy has significantly improved lung cancer patient survival, many patients show variable efficacy and resistance development. A better understanding of the drug’s action is needed to improve patient outcomes. Functional heterogeneity of the tumor microenvironment (TME) is crucial to modulating drug resistance; understanding of individual patients’ TME that impacts drug response is hampered by lack of appropriate models. Lung organotypic tissue slice cultures (OTC) with patients’ native TME procured from primary and brain-metastasized (BM) non-small cell lung cancer (NSCLC) patients were treated with Pembrolizumab and/or beta-glucan (WGP, an innate immune activator). Metabolic tracing with 13C6-Glc/ …
Neonicotinoids Can Cause Arrested Pupal Ecdysis In Lepidoptera,
2021
Missouri University of Science and Technology
Neonicotinoids Can Cause Arrested Pupal Ecdysis In Lepidoptera, Niranjana Krishnan, Russell A. Jurenka, Steven P. Bradbury
Biological Sciences Faculty Research & Creative Works
Recently, we reported a novel mode of action in monarch butterfly (Danaus plexippus) larvae exposed to neonicotinoid insecticides: arrest in pupal ecdysis following successful larval ecdysis. In this paper, we explore arrested pupal ecdysis in greater detail and propose adverse outcome pathways to explain how neonicotinoids cause this effect. Using imidacloprid as a model compound, we determined that final-instar monarchs, corn earworms (Helicoverpa zea), and wax moths (Galleria mellonella) showed high susceptibility to arrested pupal ecdysis while painted ladies (Vanessa cardui) and red admirals (Vanessa atalanta) showed low susceptibility. Fall …
Bacteria Assessment Of Soil Samples In Houston Watersheilds; Impact Of Heavy Metal, And Stress Responses In An Eukaryotic Co-Culture System.,
2021
Texas Southern University
Bacteria Assessment Of Soil Samples In Houston Watersheilds; Impact Of Heavy Metal, And Stress Responses In An Eukaryotic Co-Culture System., Folasade Adedoyin
Dissertations (2016-Present)
Houston has a complex watershed in which bayous intersect one another making the city prone to flooding, as evidenced by the 2017 Hurricane Harvey flood. We sought to evaluate bacterial population dynamics in Houston watershed soils pre- and post-Hurricane Harvey; additionally, we evaluated population dynamics in neighboring, downstream bayous ~ 1 year later in the summer and winter of 2018. This study quantified bacterial loads for pre-Hurricane Harvey (June 2017) and post-hurricane Harvey (November 2017) soil samples, as well as competitive samples from one year later [summer (June 2018) and winter (November) 2018]. Unexpectedly, bayous closer to Houston’s densely populated …
Investigating The Effects Of Perfluorooctanoic Sulfonate (Pfos) And Ethanol On Fatty Liver Disease Using A Modified Niaaa Model.,
2021
University of Louisville
Investigating The Effects Of Perfluorooctanoic Sulfonate (Pfos) And Ethanol On Fatty Liver Disease Using A Modified Niaaa Model., Tyler Charles Gripshover
Electronic Theses and Dissertations
Perfluoroalkyl Substances (PFAS) are a family of man-made, surfactant-like compounds that are a major environmental contaminant. A multitude of studies have indicated that PFAS are able to induce fatty liver disease and modulate lipid metabolism. However, the distinct mechanism of PFAS influence on the liver and metabolism disruption remains to be elucidated. On the other hand, it is well documented that alcohol consumption has various adverse health impacts including fatty liver disease and subsequent progression to more adverse liver states. To date, there are no published studies on whether PFAS and alcohol can jointly exacerbate fatty liver progression or interact …
Toxicology Of Chemical Stress To Monarch Butterflies (Danaus Plexippus L.),
2021
University of Nebraska-Lincoln
Toxicology Of Chemical Stress To Monarch Butterflies (Danaus Plexippus L.), Annie Krueger
Department of Entomology: Dissertations, Theses, and Student Research
Monarch butterfly (Danaus plexippus L.) population declines have caught the attention of the country and prompted nationwide conservation initiatives. The United States Fish and Wildlife Service has identified insecticide exposure and loss of milkweed (Asclepias spp.) reproductive habitat as primary threats to the monarch. In the Midwestern US, milkweed largely occurs around cropland borders where there may be a spatial and temporal overlap of monarch larvae, insecticide usage, and fertilizer applications. In this study, the acute toxicity and sub-lethal effects on growth and diet consumption of two commonly used pyrethroid insecticides, bifenthrin and beta-cyfluthrin, were characterized …
Cellular Toxicity Of Malonato (Ethylenediamine) Platinum (Ii) In Models Of Cancer,
2021
Western Kentucky University
Cellular Toxicity Of Malonato (Ethylenediamine) Platinum (Ii) In Models Of Cancer, Sidikat Olanrewaju Olajuwon
Masters Theses & Specialist Projects
Platinum(II) compounds including the three FDA approved drugs, cisplatin, carboplatin, and oxaliplatin, are composed of two structural components, a leaving ligand, and a non-leaving ligand, each attached to a central platinum(II) atom. All bifunctional platinum compounds studied have similar mechanisms of initiating cell death. However, their efficacy as chemotherapeutics depends on the tissues in which the cancer originates. No model has explained these tissue-specific efficacies. We hypothesized that the efficacies of these platinum compounds vary due to structural differences in the leaving ligands. To test the influence of the leaving ligand on cellular survival, novel platinum compounds were synthesized, and …
