Toxicology Commons^™

Impact Of Sodium Dichloroacetate Alone And In Combination Therapies On Lung Tumor Growth And Metastasis, Aya Mudhafar A. Al-Azawi

Theses

Lung cancer is the second most common form of cancer with the highest mortality rate worldwide in 2020 despite the advances in targeted- and immuno-therapies. Metabolic reprogramming has been recognized as an essential emerging cancer hallmark in which altered metabolic pathways represent an attractive therapeutic target. Sodium Dichloroacetate (DCA), a pyruvate dehydrogenase kinase (PDK) inhibitor, effect has been investigated in various tumors. Building on the already published data, this pre-clinical study aims to explore the anticancer potential of DCA in lung cancer alone and in combination with chemo- and targeted therapies using two non-small cell lung cancer (NSCLC) cell lines …

Do Environmental Toxins Predict Violent Crimes?, Tyler Stahl

Symposium of Student Scholars

Do chemical pollutants that persistent in the environment and bioaccumulate in the body affect human health and behavior? Could these Persistent, Bioaccumulative, and Toxic (PBT) chemicals play a role in the cause of violent crimes due to deterioration of mental and cognitive functions? In the past, Mercury, a PBT chemical, has been shown in salmon to be associated with aggression. Could similar aggression occur in humans exposed to mercury through a toxic spill? Two sources of data are utilized in this analysis. The Environmental Protection Agency’s (EPA) Annual Toxic Release Inventory publishes data on toxic releases into the environment and …

In Vivo Antidiabetic Activities Of Polyherbal Extracts Against Streptozotocin-Nicotinamide Induced Type 2 Diabetic Mice Model, Pradnya Jagtap, Yashashri Nimbalkar, Vidya Patil, Ravindra Y. Patil

Tanzania Journal of Science

Diabetes mellitus is a chronic metabolic disorder and rapidly increasing cases of diabetes throughout the world are major concerns in both developed and developing countries. Currently available oral antidiabetic medicines have limitations in efficacy and safety, which in conjunction with the emergence of diabetes mellitus as a global epidemic have aided the popularity of alternative therapies to manage the disease safely and more efficiently. Herbal medicines are accepted as alternative therapies for blood sugar control. Gymnema sylvestre, Boerhavia diffusa, Tinospora cordifolia, and Argyreia nervosa are some potent herbs used for the management of diabetes. Mixing of these plants in different …

Prunus Africanus Herbal Extracts Reverse And Ameliorate The Histological And Histomorphometric Changes In Testosterone-Induced Benign Prostate Hyperplasia Rat Models, Felix P. William, Denis A. Russa

Tanzania Journal of Science

Benign prostatic hyperplasia (BPH) is the most common benign proliferative disease among men during aging. The herbal extract of P. africanus has been used for the treatment of BPH since time immemorial. However microstructural changes of this extract to the prostate of animal or human models are still elusive. Therefore, histological and histomorphometric changes in rats with testosterone-induced BPH due to P. africanus herbal extracts were investigated. Twenty-eight male Wistar rats (200 ± 50 g) were divided into four groups each with 7 rats. Group 1 (Negative control) was given 2 ml/day of olive oil subcutaneously and 2 ml/day of …

Childhood Exposure To Per- And Polyfluoroalkyl Substances (Pfas) And Neurobehavioral Domains In Children At Age 8 Years, Ann M. Vuong, Kimberly Yolton, Changchun Xie, Kim N. Dietrich, Joseph M. Braun, Glenys M. Webster, Antonia M. Calafat, Bruce P. Lanphear, Aimin Chen

Environmental & Global Health Faculty Research

Background: Toxicological studies have raised concerns regarding the neurotoxic effects of per- and polyfluoroalkyl substances (PFAS). However, observational evidence from human studies investigating the association between childhood PFAS and neurobehavior is limited and remains unclear. Objectives: To examine whether childhood PFAS concentrations are associated with neurobehavior in children at age 8 years and whether child sex modifies this relationship. Methods: We used data from 208 mother-child dyads in the Health Outcomes and Measures of the Environment (HOME) Study, a prospective pregnancy and birth cohort (Cincinnati, OH, USA). We quantified PFAS in child serum at 3 and 8 years. We assessed …

Cocaine, Cocaethylene And Ethanol In Postmortem Blood Submitted To The New York City Office Of Chief Medical Examiner, Jada T. Alexis

Student Theses

Cocaine overdose deaths are on the rise in the United States. This increase may be due to the co-consumption of cocaine with other drugs. When cocaine is ingested in conjunction with alcohol, a specific metabolite called cocaethylene forms. Information regarding cocaethylene, specifically its interpretive value in post-mortem cases, has not been widely studied. Data from the Office of the Chief Medical Examiner in New York City revealed that there were 195 cases in 2017 positive for cocaethylene. These cases were most likely to involve black (42.1%) males (79.5%) in Manhattan (28.2%) aged 50 - 59 (34.4%). Most of the cases …

Innate Immune Activation By Checkpoint Inhibition In Human Patient-Derived Lung Cancer Tissues, Teresa W. M. Fan, Richard M. Higashi, Huan Song, Saeed Daneshmandi, Angela L. Mahan, Matthew S. Purdom, Therese J. Bocklage, Thomas A. Pittman, Daheng He, Chi Wang, Andrew N. Lane

Center for Environmental and Systems Biochemistry Faculty Publications

Although Pembrolizumab-based immunotherapy has significantly improved lung cancer patient survival, many patients show variable efficacy and resistance development. A better understanding of the drug’s action is needed to improve patient outcomes. Functional heterogeneity of the tumor microenvironment (TME) is crucial to modulating drug resistance; understanding of individual patients’ TME that impacts drug response is hampered by lack of appropriate models. Lung organotypic tissue slice cultures (OTC) with patients’ native TME procured from primary and brain-metastasized (BM) non-small cell lung cancer (NSCLC) patients were treated with Pembrolizumab and/or beta-glucan (WGP, an innate immune activator). Metabolic tracing with ¹³C₆-Glc/ …

Neonicotinoids Can Cause Arrested Pupal Ecdysis In Lepidoptera, Niranjana Krishnan, Russell A. Jurenka, Steven P. Bradbury

Biological Sciences Faculty Research & Creative Works

Recently, we reported a novel mode of action in monarch butterfly (Danaus plexippus) larvae exposed to neonicotinoid insecticides: arrest in pupal ecdysis following successful larval ecdysis. In this paper, we explore arrested pupal ecdysis in greater detail and propose adverse outcome pathways to explain how neonicotinoids cause this effect. Using imidacloprid as a model compound, we determined that final-instar monarchs, corn earworms (Helicoverpa zea), and wax moths (Galleria mellonella) showed high susceptibility to arrested pupal ecdysis while painted ladies (Vanessa cardui) and red admirals (Vanessa atalanta) showed low susceptibility. Fall …

Investigating The Effects Of Perfluorooctanoic Sulfonate (Pfos) And Ethanol On Fatty Liver Disease Using A Modified Niaaa Model., Tyler Charles Gripshover

Electronic Theses and Dissertations

Perfluoroalkyl Substances (PFAS) are a family of man-made, surfactant-like compounds that are a major environmental contaminant. A multitude of studies have indicated that PFAS are able to induce fatty liver disease and modulate lipid metabolism. However, the distinct mechanism of PFAS influence on the liver and metabolism disruption remains to be elucidated. On the other hand, it is well documented that alcohol consumption has various adverse health impacts including fatty liver disease and subsequent progression to more adverse liver states. To date, there are no published studies on whether PFAS and alcohol can jointly exacerbate fatty liver progression or interact …

Toxicology Of Chemical Stress To Monarch Butterflies (Danaus Plexippus L.), Annie Krueger

Department of Entomology: Dissertations, Theses, and Student Research

Monarch butterfly (Danaus plexippus L.) population declines have caught the attention of the country and prompted nationwide conservation initiatives. The United States Fish and Wildlife Service has identified insecticide exposure and loss of milkweed (Asclepias spp.) reproductive habitat as primary threats to the monarch. In the Midwestern US, milkweed largely occurs around cropland borders where there may be a spatial and temporal overlap of monarch larvae, insecticide usage, and fertilizer applications. In this study, the acute toxicity and sub-lethal effects on growth and diet consumption of two commonly used pyrethroid insecticides, bifenthrin and beta-cyfluthrin, were characterized …

Bacteria Assessment Of Soil Samples In Houston Watersheilds; Impact Of Heavy Metal, And Stress Responses In An Eukaryotic Co-Culture System., Folasade Adedoyin

Dissertations (2016-Present)

Houston has a complex watershed in which bayous intersect one another making the city prone to flooding, as evidenced by the 2017 Hurricane Harvey flood. We sought to evaluate bacterial population dynamics in Houston watershed soils pre- and post-Hurricane Harvey; additionally, we evaluated population dynamics in neighboring, downstream bayous ~ 1 year later in the summer and winter of 2018. This study quantified bacterial loads for pre-Hurricane Harvey (June 2017) and post-hurricane Harvey (November 2017) soil samples, as well as competitive samples from one year later [summer (June 2018) and winter (November) 2018]. Unexpectedly, bayous closer to Houston’s densely populated …

Cellular Toxicity Of Malonato (Ethylenediamine) Platinum (Ii) In Models Of Cancer, Sidikat Olanrewaju Olajuwon

Masters Theses & Specialist Projects

Platinum(II) compounds including the three FDA approved drugs, cisplatin, carboplatin, and oxaliplatin, are composed of two structural components, a leaving ligand, and a non-leaving ligand, each attached to a central platinum(II) atom. All bifunctional platinum compounds studied have similar mechanisms of initiating cell death. However, their efficacy as chemotherapeutics depends on the tissues in which the cancer originates. No model has explained these tissue-specific efficacies. We hypothesized that the efficacies of these platinum compounds vary due to structural differences in the leaving ligands. To test the influence of the leaving ligand on cellular survival, novel platinum compounds were synthesized, and …

Computational Analysis Of Type 3 Iodothyronine Deiodinase: Potential Inhibitors, Substrate Binding, And Dimer Structure, Eric Scott Marsan

Chemistry & Biochemistry Theses & Dissertations

Thyroid hormones (THs) in mammalian tissues are crucial for development and maintaining metabolic homeostasis. Iodothyronine deiodinases (Dios) remove iodines from THs by a selenocysteine (Sec) residue, which either activates or inactivates them. Halogen bonding (XB) has been proposed to describe the interaction between the Se and I atoms of the T4-Dio complex. Disruption of TH homeostasis by xenobiotics, such as polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) can cause deleterious effects on the endocrine system. Experimental studies have indicated that PBDEs and PCBs could disrupt TH homeostasis by inhibiting Dio through XB formation. However, no current quantitative study exists …

The Effects Of Agrochemical Pesticide Applications On Target And Off-Target Species In Aquatic Ecosystems, Bryan K. Delius

USF Tampa Graduate Theses and Dissertations

The increase in the global human population is driving agricultural expansion, which increases the likelihood and degree by which agrochemicals impact aquatic systems. It is projected that by 2100 the global human population will exceed 11 billion, and to feed the increased human population, the necessary increase in agricultural infrastructure is estimated to double irrigation, nearly triple fertilizer applications, and increase pesticide applications by tenfold. It stands to reason that wetlands impacts from agriculture are also likely to increase as the industry expands its operation to meet the globally growing demand for food.

Wetlands are highly productive habitats that not …

Corrigendum To “Novel Uplc-Ms/Ms Method For Standardization Of Niazimicin Content In Edible Seeds And Leaves Of Moringa Oleifera Lam” [J Food Drug Anal 29 (2021) 128-143]

Journal of Food and Drug Analysis

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Corrigendum To “Determination Of Glycidyl Esters And 3-Mcpd Esters In Edible Oils By Sample Pretreatment With The Combination Of Lipase Hydrolysis And Modified Quechers For Gc-Ms Analysis” [J Food Drug Anal 29 (2021) 153-167]

Journal of Food and Drug Analysis

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Pepsin-Digested Chicken-Liver Hydrolysate Attenuates Hepatosteatosis By Relieving Hepatic And Peripheral Insulin Resistance In Long-Term High-Fat Dietary Habit, Yi-Hsieng Samuel Wu, Yi-Ling Lin, Wen-Yuan Yang, Sheng-Yao Wang, Yi-Chen Chen

Journal of Food and Drug Analysis

This study aims to clarify the effects of chicken liver hydrolysates (CLHs) on long-term high-fat diet (HFD)-induced insulin resistance (IR) and hepatosteatosis in mice. In vitro, the 400 mM oleic acid (OA)-added medium successfully stimulated the cellular steatosis on FL83B cells, and the cellular steatosis was attenuated ( p < 0.05) by supplementing with CLHs (4 mg/L). In vivo, the effects of CLHs on IR and hepatosteatosis development were tested in 20-week HFD-fed mice. HFD-induced increases in final body weight, but body weight gains of mice were decreased ( p < 0.05) by supplementing CLHs. Elevated ( p < 0.05) serum aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), free fatty acids (FFAs), triglyceride (TG), total cholesterol (TC), and fasted glucose values in HFD-fed mice decreased ( p < 0.05) by supplementing CLHs. Both results of hepatic steatosis and fibrotic scores also indicated the retardation ( p < 0.05) of the hepatosteatosis in cotreated groups. Moreover, the CLH supplementation sustained ( p < 0.05) hepatic and peripheral insulin signal sensitivity in HFD-fed mice. CLH supplementation could ameliorate hepatic lipid deposition, hepatic/peripheral IR in a long-term high-fat dietary habit, and also improve the universal glucose homeostasis by upregulating hepatic and peripheral insulin sensitivities.

Adverse Effects On Birth Weight Of Parental Illegal Drug Use During Pregnancy And Within Two Years Before Pregnancy, Ching-Heng Lin, Wei-Szu Lin, I-An Wang, Jui Hsu, Shiow-Ing Wu, Chuan-Yu Chen

Journal of Food and Drug Analysis

This study investigates possible links between maternal illegal drug use during pregnancy and up to two years before pregnancy with birth weight (BW), and explores the potential role of paternal illegal drug use on low birth weight. A population-based retrospective cohort study was conducted that linked four national databases in Taiwan. A total of 1,698 subjects with a criminal record of schedule I or II illegal drug use within two years before pregnancy were enrolled as the drug-exposed group, and 16,980 matched subjects were enrolled as the unexposed group. Multivariate analysis of BW found a decrease of 108.63 g (95% …

Simultaneously Qualitative And Quantitative Analysis Of The Representative Components In Kadsura Heteroclita Stem By Uhplc-Q-Orbitrap Hrms, Qian-Qian Liu, Bin Li, Kang Sun, Cai-Hong Li, Nuzhat Shehla, Yu-Pei Yang, Liang Cao, Wei Wang, Rong-Xia Liu

Journal of Food and Drug Analysis

Kadsura heteroclita (Roxb) Craib stem (KHS) is a medicinal plant used for the treatment of rheumatism arthritis diseases in Tujia ethnomedicine. Thus far, the complex chemical compositions in KHS are not clear, and the levels of the major compounds in KHS are not well understood. In this study, a novel UHPLC-Q-Orbitrap HRMS method was established for the simultaneous quali-quantitative analysis of KHS. A total of 204 compounds were identified, including triterpenoids, lignans, sesquiterpenes, fatty acids, phenolic acids, and flavonoids, more than 100 of which were first discovered in KHS. Using the same method, 12 representative bioactive components were successfully quantified. …

Characterization Of L-Theanine In Tea Extracts And Synthetic Products Using Stable Isotope Ratio Analysis, Matteo Perini, Silvia Pianezze, Luca Ziller, Federica Camin

Journal of Food and Drug Analysis

L-theanine involves a great number of health benefits and dietary supplements containing this molecule are becoming increasingly popular. There is, therefore, a growing need to find ways to discriminate between natural L-theanine extracted from tea leaves and the cheaper, synthetic one obtained using specific bacterial enzymes. A first attempt of stable isotope ratio analysis characterization of the possible synthetic adulterant L-theanine (δ¹³C of -14.3±1.5‰), obtained from vegetable substrates with C4 photosynthetic cycle, and of the more expensive natural L-theanine (δ¹³C of -24.4±1.3‰), extracted from Camellia sinensis plants with C3 photosynthetic cycle, is reported here .