Pharmacology, Toxicology and Environmental Health Commons^™

Analytical Method Development And Validation For Iron And Ascorbic Acid In Ferrous Bisglyciante Effervescent Tablets By Colorimetry And Rp-Hplc., Vasantharaju Sg

The Thai Journal of Pharmaceutical Sciences

A sensitive colorimetric and reverse phase high performance liquid chromatographic method was developed for estimation of total iron and ascorbic acid in ferrous bisglycinate effervescent tablets. A colorimetric method was developed for total iron us...

The Reform Of Drug Patent System In Thailand, Wanna Sriviriyanupap

The Thai Journal of Pharmaceutical Sciences

Drug patent system has a direct impact on the health care cost and the access of the drugs of people. Previously, the drug patent system in Thailand encounters the problems both efficiency and quality of patent approval. This study aims to analyze the pr.

Effects Of The Standardized Extract Of Centella Asiatica Eca233 On Hepatic Phase Ii Drug-Metabolizing Enzymes In Rats, Pitchayapa Seeka, Nuansri Niwattisaiwong, Mayuree H. Tantisira, Songpol Chevapat, Kridsada Anuntawuttikul, Khemchat Apipalakul, Somsong Lawanprasert

The Thai Journal of Pharmaceutical Sciences

The standardized extract of C. asiatica ECa233 (ECa 233) was shown to possess neuroprotective and wound healing effects. The purpose of this study was to investigate effects ECa 233 on the activities of phase II drug metabolizing enzymes including UDP-gl.

Application Of Ion-Pair Complexation Reaction For The Spectrophotometric Determination Of Clarithromycin In Pharmaceutical Formulations, Sameer A. M. Abdulrahman

The Thai Journal of Pharmaceutical Sciences

Two simple, rapid, sensitive, selective and extraction-free spectrophotometric methods have been developed for the determination of clarithromycin (CLR) both in pure form and in pharmaceutical formulations. The developed methods are based on ion-pair com.

High-Performance Thin-Layer Chromatography Analysis Of (E)-4-(3?,4?-Dimethoxyphenyl)But-3-En-1-Ol (Compound D) In Zingiber Cassumunar Roxb Rhizome Extract: An Application On Genetic Differentiation, Prasan Tangyuenyongwatana, Piyarat Parinyapong Chareonsap, Wandee Gritsanapan

The Thai Journal of Pharmaceutical Sciences

Objectives: This study focused on developing high performance thin-layer chromatography (HPTLC) quantitative analysis method for compound D, a major anti-inflammatory compound in Zingiber cassumunar extract. In addition, the amplified fragment length pol.

Hepatoprotective And Hepatoregenerative Therapeutic Effects Of Polyherbal Medicine Heparmin Tm On Rattus Norvegicus Wistar With Liver Fibrosis, Diana Lyrawati, Amalia Ghassani Muslimah, Dewi Laksmita, Dian Indrawati Santoso, Erlien Lindawati Poernomo, Karina Larasati, Lina Zahrotus Sajidah, Oktavia Rahayu Adianingsih, Rosida Dewi Agusningtyas, Mudjiwijono Handaru Eko, Bogi Pratomo Wibowo

The Thai Journal of Pharmaceutical Sciences

Chronic injury severely impairs liver regeneration through excess inflammation, scarring and epithelial abnormalities, since injured cells may shift response from pro-regeneration into pro-fibrosis which leads to cirrhosis and total liver failure. An Ind.

Deubiquitinases In Ubiquitin Homeostasis And Disease, Gorica Ristic

Wayne State University Dissertations

Protein quality control (PQC) is indispensable for normal cellular functions, ensuring proteins are properly folded and removing those proteins that are functioning aberrantly. Perturbations in PQC can lead to various malignancies, neurodegeneration and neurological diseases. The Ubiquitin-Proteasome Pathway (UPP) is one important pathway in PQC, it relies on ubiquitination-dependent post-translational modification to selectively degrade misfolded or short-lived protein. Ubiquitination is reversed by the action of proteases known as deubiquitinating enzymes (DUBs). By hydrolyzing ubiquitin linkages, DUBs are responsible for cleaving and activating newly produced ubiquitin molecules, editing poly-Ub chains, removing ubiquitin from a substrate protein and recycling mono-Ub. Here, I …

Protein Phosphatase 2a Function And Regulation In Insulin Resistant Human Skeletal Muscle Cells, Shukurat Sulaiman

Wayne State University Theses

Protein Phosphatase 2A (PP2A), a major serine/threonine phosphatase involved in insulin signaling pathway, plays a critical role in the development of insulin resistance and type 2 diabetes (T2D), which is characterized with hyperinsulinemic hyperglycemic condition. Moreover, majority of the glucose disposal takes place in the skeletal muscle which is also the main tissue responsible for insulin resistance. The catalytic subunit of PP2A (PP2Ac) can interact with multiple regulatory subunits and other regulatory proteins as well as substrates. These interactions are important for maintain normal PP2A function and subsequent cell signaling. We hypothesized that hyperinsulinemic hyperglycemic condition and insulin stimulation would …

Aluminum And Phthalates In Calcium Gluconate: Contribution From Glass And Plastic Packaging, Robert A. Yokel, Jason M. Unrine

Pharmaceutical Sciences Faculty Publications

Introduction: Aluminum contamination of parenteral nutrition solutions has been documented for three decades. It can result in elevated blood, bone, and whole body aluminum levels associated with neurotoxicity, reduced bone mass and mineral content, and perhaps hepatotoxicity. The primary aluminum source among parenteral nutrition components is glass-packaged calcium gluconate, in which aluminum concentration the past three decades has averaged~ 4000 [mu]g/L, compared to < 200 [mu]g/L in plastic container-packaged calcium gluconate. A concern about plastic packaging is leaching of plasticizers, including phthalates, which have the potential to cause endocrine (male reproductive system) disruption and neurotoxicity.

Methods: Aluminum was quantified in samples collected periodically over more than two years from three calcium gluconate sources used to prepare parenteral nutrition solutions; two packaged in glass (from France and the US) and one in plastic …

Organ-Specific Contribution Of P450 Enzymes To Bioactivation And Acute Respiratory Tract Toxicity Of Naphthalene, Nataliia Kovalchuk

Legacy Theses & Dissertations (2009 - 2024)

Naphthalene (NA) is an omnipresent air pollutant and respiratory toxicant and a possible human lung carcinogen. NA induces cytotoxicity in airways following bioactivation by CYP (P450) enzymes. The overall goal of this study was to examine the relative contributions of hepatic and pulmonary CYPs to bioactivation, disposition and NA-induced pneumotoxicity in vivo. Substantial differences in the expression and enzymatic activity of P450s occur in various organs in human populations, which could modify susceptibility to NA-induced airway damage. The central hypothesis for this study was that NA has the potential to cause lung toxicity in humans and that the metabolism of …

Biomarkers And Metabolic Mechanisms Of Tobacco Smoke-Induced Lung Disease, Matthew Alan Hartog

Legacy Theses & Dissertations (2009 - 2024)

The overall goal of the research conducted in this dissertation is to determine the role of cytochrome (CYP) P450-mediated xenobiotic bioactivation in the development of tobacco smoke-induced lung toxicity. The central hypothesis of this work is that CYP-mediated bioactivation of specific tobacco smoke constituents is an important contributor to the generation of tobacco smoke-induced lung inflammation and injury. The specific aims are 1) to demonstrate that CYP-mediated metabolism contributes to the onset of lung inflammation following tobacco smoke inhalation; 2) to demonstrate that specific tobacco smoke constituents undergo CYP-mediated bioactivation in vivo; 3) to demonstrate that CYP-mediated bioactivation of tobacco …

Wheezing & Food Allergies In Infants Delivered Via Cesarean Section : The Upstate Kids Study, Temilayo Elizabeth Adeyeye

Legacy Theses & Dissertations (2009 - 2024)

This dissertation sought to assess the effect of cesarean delivery (CD) on the risk of developing wheezing or food allergies in early childhood; and whether breastfeeding mediates the association between CD and the outcomes. We also aimed to highlight the differences in immunoglobulin concentrations by mode of delivery (MOD) in newborn dried blood spots (DBS), and the subsequent development of wheezing and food allergies in infants during the first three years of life. Data from the Upstate KIDS cohort was analyzed. Modified Poisson regression was used to compare the risks for wheezing and food allergies by MOD. We used a …

Understanding The Genotoxicity Of Silver Nanoparticles And The Chemoprevention Of Pomegranate Extract, Sameera Nallanthighal

Legacy Theses & Dissertations (2009 - 2024)

The use of silver nanoparticles (AgNPs) in a wide variety of consumer products (i.e. toothpastes, food containers, dietary supplements and garments) for their antimicrobial properties can lead to potential oral exposure in humans. To enhance their stability, AgNPs are coated with capping agents such as citrate and polyvinylpyrrolidone (PVP). Despite the lack of significant general toxicity based on hematology, blood chemistry and histology evaluations, the potential genotoxic effects of AgNPs cannot be ruled out and have to be addressed. Studies examining the genotoxic risks of AgNPs are needed because genotoxicity is a strong indicator of cancer risk. Here we examined …

Effects Of Antibiotic Treatment On The Expression Of Hepatic And Intestinal Cytochrome P450, Bruno Pereira De Carvalho

Legacy Theses & Dissertations (2009 - 2024)

Expression of metabolic enzymes Cytochrome P450 (CYP) is highly affected by drugs, diseases, age and gender. The Intestinal microflora has been suggested to play a role in regulating CYP expression and antibiotic treatments would lead to changes in level and composition of microbiota in mouse gut. This study aims to analyze CYP expression changes in the liver and small intestine resulted from the reduction of intestinal microflora by antibiotics. Impacts on bile acid signaling were also analyzed for a better comprehension of the mechanisms involved in this modulation. Female C57BL/6J mice (9- to 13-week old) were treated with ampicillin (AMP) …

Sources, Pathways, And Distribution Of Bisphenol A Diglycidyl Ether (Badge) And Its Derivatives In The Environment, Jingchuan Xue

Legacy Theses & Dissertations (2009 - 2024)

BADGE (bisphenol A diglycidyl ether) based epoxy resins have been used in a variety of applications since the 1940s. However, limited studies are available about the occurrence of BADGE and its derivatives (BADGEs) in the environment. The aims of this thesis were to determine the concentrations of BADGE and its derivatives in dental sealants and textile products from the U.S. to characterize the sources of these substances. Furthermore, concentrations of BADGEs in samples from various stages of waste water treatment plants (WWTPs) in the U.S. were also determined to investigate the environmental pathway and fate of these substances. BADGEs were …

The Effects Of Metolachlor Exposure On The Thp-1 Alveolar Monocyte And Macrophage Function, Jared S. Parmater

Dissertations and Theses @ UNI

The use of additive chemicals such as pesticides has become commonplace in agricultural practices in the Midwestern United States. Metolachlor, an herbicide used to regulate broadleaf weeds in corn and soybeans, is a widely utilized pesticide in the United States. This herbicide is effective in killing broadleaf weeds but has detrimental effects and consequences in non-target organisms. It is a frequent contaminant of ground water due to post application run-off and the air.

Inhalation constitutes one route of exposure, in addition to ingestion, adding to the list of unintended targets and consequences. This route of exposure creates an argument for …

Valproic Acid: A Neural Outgrowth Model For The Autism Spectrum Disorder, Hannah M. Bergan

Dissertations and Theses @ UNI

Autism spectrum disorder has been dramatically on the rise since the early 1990s, with one in forty-five children being diagnosed in 2014. It is known that contributing factors to autism spectrum disorder may have genetic and epigenetic origins, as well as environmental triggers. What is still unclear, however, is the impact of teratogenic drugs, consumed by the mother, have on a developing fetus in utero. How can they lead to the child developing autistic-like behaviors? The current study documents the effects of one teratogenic drug Valproic acid, a known histone deacetylase inhibitor. It establishes the role it plays on developing …

Short Term Effects On Symptoms Of Atopic Dermatitis In Children By Weather And Polution, Paolo Kinsella

Natural Sciences Student Research Presentations

This slide presentation for the Natural Sciences Poster Session at Parkland College summarizes a Korean study (Kim et al) on the effects of weather and air pollution on the severity and persistence of atopic dermatitis, a prevalent condition in Korea.

Biochemical, Structural, And Drug Design Studies Of Norovirus And Zika Virus Proteases, Ben Kuiper

Wayne State University Dissertations

Noroviruses, which are the leading cause of acute gastroenteritis, cause an estimated 677 million infections and 213,000 deaths each year worldwide. Noroviruses are classified into seven genogroups (GI-GVII); GI, GII, and GIV have been shown to be infectious in humans. However, GII noroviruses cause the majority of outbreaks (89%). No pharmacologic treatment or vaccine currently exists to treat or prevent norovirus infections.

Recently, the development of a norovirus replicon system, a murine model of norovirus infection, and the development of a biochemical protease assay have allowed for the design and development of norovirus inhibitors. However, the replicon and biochemical assay …

Patholigical And Prognostic Role Of Mdig In Pancreatic Cancer, Srinivas Ashok Kumar

Wayne State University Theses

Pancreatic cancer is a highly aggressive malignant disease having very limited therapeutic options that ultimately results in its poor prognosis. It is still elusive on the etiology and tumorigenic mechanisms of pancreatic cancer. In the present report, we provide evidence showing involvement of the mineral dust-induced gene (mdig) in the pathogenesis and prognosis of the pancreatic cancer. Using immunohistochemistry approach on human pancreatic cancer tissue microarray, we found differential expression of mdig in pancreatic adenocarcinoma and normal pancreas. Based on the staining intensities of mdig in these tissue samples, we found that 12% of the cancer tissues were strongly positive …