Preparation, Evaluation And Metabolites Study In Rats Of Novel Isoginkgetin-Loaded Tpgs/Soluplus Mixed Nanomicelles,
2020
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University,China
Preparation, Evaluation And Metabolites Study In Rats Of Novel Isoginkgetin-Loaded Tpgs/Soluplus Mixed Nanomicelles, Xue Feng, Yu-Ting Chen, Lu-Ya Li, Yu-Peng Sun, Hai-Rong Wang, Lan-Tong Zhang
Journal of Food and Drug Analysis
At present, cancer is one of the most lethal diseases in the world, and researchers are committed to developing effective anticancer drugs. Isoginkgetin (IGG) is a kind of biflavone with the potential to treat cancer due to the features of altering the cell cycle and inhibiting tumor cell infiltration. However, its solubility, absorbability and bioavailability are poor, so in this study, IGG was prepared into mixed nanomicelles and evaluated in vitro and in vivo. After condition optimization, IGG-loaded TPGS/soluplus mixed nanomicelles with particle size of 62.34 ± 1.10 nm, entrapment efficiency of 96.92 ± 0.66% and drug loading of 2.42 …
Rapid Identification Of Nicotine In Electronic Cigarette Liquids Based On Surface-Enhanced Raman Scattering,
2020
Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan
Rapid Identification Of Nicotine In Electronic Cigarette Liquids Based On Surface-Enhanced Raman Scattering, Jun-Yi Chien, Yong-Chun Gu, Hsin-Mei Tsai, Chun-Hao Liu, Chia-Yuan Yen, Yuh-Lin Wang, Juen-Kai Wang, Chi-Hung Lin
Journal of Food and Drug Analysis
Nicotine-containing electronic cigarette liquid (e-liquid) is prohibited in many countries, creating requirements for rapid detection approaches for on-site inspection or screening for large amounts of samples. Here, we demonstrate a simple way to identify nicotine using surface-enhanced Raman scattering (SERS) with substrates made of silver nanoparticle arrays imbedded in anodic aluminum oxide nanochannels (Ag/AAO). Compared with the reported colloidal nanoparticle-based SERS, that required serial dilutions to enable colloid aggregation in the viscous e-liquid, a small amount of undiluted e-liquid sample can be directly added onto our solid-phase Ag/AAO substrate without any pre-treatment. The sensitivity of our SERS measurements is 2e3 …
A Multi-Analyte Lc-Ms/Ms Method For Screening And Quantification Of Nitrosamines In Sartans,
2020
Food and Drug Administration, Ministry of Health and Welfare, Taipei,Taiwan
A Multi-Analyte Lc-Ms/Ms Method For Screening And Quantification Of Nitrosamines In Sartans, Shu-Han Chang, Ching-Chia Chang, Li-Jing Wang, Wei-Ching Chen, Shu-Yu Fan, Chi-Zong Zang, Ya-Hui Hsu, Mei-Chih Lin, Su-Hsiang Tseng, Der-Yuan Wang
Journal of Food and Drug Analysis
An incident of sartan medicine contamination was notified by Europe in June 2018. The contaminant was identified as a probable carcinogenic nitrosamine and the recalls of sartan medicines were soon made. Since then, more nitrosamine contaminants in sartan medicines were reported. To broaden the applicability and variety in nitrosamine determination, a multi-analyte method is required. In this study, a feasible and sensitive multi-analyte LC-MS/MS method for determination of 12 nitrosamines in sartans was established, where the active pharmaceutical ingredients and final products merchandised in Taiwan were also examined. Chromatographic separation was achieved on an Xselect® HSS T3 column (15 cm …
Anti-Inflammatory Effects Of Peptides From A Marine Algicolous Fungus Acremonium Sp. Ntu492 In Bv-2 Microglial Cells,
2020
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan
Anti-Inflammatory Effects Of Peptides From A Marine Algicolous Fungus Acremonium Sp. Ntu492 In Bv-2 Microglial Cells, George Hsiao, Shih-Wei Wang, Yin-Ru Chiang, Wei-Chiung Chi, Yueh-Hsiung Kuo, Do Anh Phong, Chia-Yu Chen, Tzong-Huei Lee
Journal of Food and Drug Analysis
Located in tropical and subtropical region, Taiwan is an island with high algal species diversity. In this study, a number of fungal strains were isolated from marine macroalgae collected from northeastern intertidal zone of Taiwan. Preliminary anti-inflammatory screening has shown that the methanolic extracts of solid fermented products of the red alga Mastophora rosea-derived fungal strain Acremonium sp. NTU492 exhibited significant bioactivity. In an attempt to disclose the active principles from this fungal strain, a series of separation and purification was thus undertaken, which has led to the isolation and characterization of seven compounds including four new peptides, namely acrepeptins …
A Rapid Strategy For Screening High-Efficiency Pcsk9 Inhibitors From Ginkgo Biloba Leaves By Ligand Fishing, Hplc-Q-Tof-Ms And Interdisciplinary Assay,
2020
Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China
A Rapid Strategy For Screening High-Efficiency Pcsk9 Inhibitors From Ginkgo Biloba Leaves By Ligand Fishing, Hplc-Q-Tof-Ms And Interdisciplinary Assay, Li Li, Meng-Lin Fan, Ya-Nan Li, Ya-Xuan Huang, Xiu-Feng Liu, Ji-Hua Liu
Journal of Food and Drug Analysis
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an attractive target for new cholesterol-lowering drug development. Here, we developed a method integrating ligand fishing, HPLC-Q-TOF-MS and interdisciplinary assay, aiming to explore potential PCSK9 inhibitors from mixtures rapidly and accurately. PCSK9 was expressed and purified firstly, and then the recombined PCSK9 was coated on the surface of magnetic beads (MBs). The PCSK9-immobilized MBs (PCSK9-MBs) were used for ligand fishing combined with HPLC and Q-TOF-MS/MS. Ginkgo biloba leaves (GBL), an herbal medicine widely used in Asia and Europe with good efficacy in treatment of hypercholesterolemia, were chosen as an illustration for ligand fishing. …
Analysis Of Flavor And Other Metabolites In Lemon Juice (Citrus Limon) From Huanglongbing-Affected Trees Grafted On Different Rootstocks,
2020
Citrus Research and Education Center, Food Science and Human Nutrition, University of Florida, Lake Alfred, Florida, USA
Analysis Of Flavor And Other Metabolites In Lemon Juice (Citrus Limon) From Huanglongbing-Affected Trees Grafted On Different Rootstocks, Laura Reuss, Shi Feng, Wei-Lun Hung, Qibin Yu, Frederick G. Gmitter Jr, Yu Wang
Journal of Food and Drug Analysis
Flavor perception integrates sensory input from chemical receptors triggered by taste- and aroma-active metabolites to contribute to food flavor quality. Many factors alter flavor quality. Disease affects aroma and flavonoid constituents, causing off-flavors in plants. Huanglongbing (HLB) disease negatively affects citrus, although lemons are more tolerant. Lemon juice quality of HLB-affected fruits is not well studied. Lemon juice aroma-active compounds were profiled in this study using gas chromatography/mass spectrometry-olfactometry, and other metabolites contributing to overall flavor quality were investigated. Lemon juice from different rootstocks was discriminated using the metabolic profile. Flavor and other lemon juice metabolites also distinguished symptomatic from …
Chemometric Authentication Of Pu'er Teas In Terms Of Multielement Stable Isotope Ratios Analysis By Ea-Irms And Icp-Ms,
2020
College of Food Science, Southwest University, Chongqing, China
Chemometric Authentication Of Pu'er Teas In Terms Of Multielement Stable Isotope Ratios Analysis By Ea-Irms And Icp-Ms, Honglin Liu, Yitao Zeng, Xin Zhao, Huarong Tong
Journal of Food and Drug Analysis
In this work, the stable isotope ratios of carbon, nitrogen, hydrogen, oxygen, and mineral elements and their stoichiometric methods were examined as possible factors that could certify Chinese tea based on its production years. A total of 43 multi-element stable isotope ratios of Xiangzhujing Pu'er tea in five production years were determined through inductively coupled plasma mass spectrometry (ICP-MS) and elemental analyzer-isotope ratio mass spectrometry (EA-IRMS) methods. Two unsupervised learning techniques (principal component analysis and hierarchical clustering analysis) and three supervised learning techniques (partial least squares discriminant analysis [PLS-DA], back-propagation artificial neural network [BP-ANN], and linear discriminant analysis [LDA]) were …
The Chemical Aspects Of Raman Spectroscopy: Statistical Structure-Spectrum Relationship In The Analyses Of Bioflavonoids,
2020
National Chiayi University, Taiwan
The Chemical Aspects Of Raman Spectroscopy: Statistical Structure-Spectrum Relationship In The Analyses Of Bioflavonoids, Chih-Hsien Wang, Chia-Chi Huang, Wenlung Chen, Yen-Shi Lai
Journal of Food and Drug Analysis
Raman spectroscopy has been accepted as a useful tool for the characterization of natural products. However, to identify a specific compound in a mixture sample of natural products using Raman spectra alone is highly challenging if not impossible. We demonstrated an effective solution to such issues using a method combining statistical Raman spectroscopy and Mass spectrometry. The method was validated with a successful application to the identification of the major anthocyanin components in a purple yam (Dioscorea purpurea) extract. Of particular interest is that statistical grouping of the bioflavonoid standards that formed the database of this study was found to …
Screening For Malachite Green Contamination On Live Fish Skin With Chewing Gum Based Viscoelastic Sers Sensor,
2020
Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China
Screening For Malachite Green Contamination On Live Fish Skin With Chewing Gum Based Viscoelastic Sers Sensor, Wan-Li Fan, Shi-Wei Yang, Dong-Mei Wang, Zheng-Jun Gong, Mei-Kun Fan
Journal of Food and Drug Analysis
Malachite green (MG), a prohibited but still found antimicrobial in aquafarm and during live fish shipping, is a hot target in food safety screening. Herein, a novel chewing gum based flexible SERS (G-SERS) sensor was proposed for rapid sampling and detection of MG on live fish skin. The whole analysis takesmin, while the limit of detection for MG is 0.73 pg. Different from other reports, MG contaminated live fish was monitored daily with the G-SERS sensor, during which the fish was firstly raised in 0.5 ppm MG solution for one day, followed by freshwater for a week. It was found …
Uplc-Qtof-Ms Fingerprinting Combined With Chemometrics To Assess The Solvent Extraction Efficiency, Phytochemical Variation, And Antioxidant Activities Of Beta Vulgaris L.,
2020
Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, USA
Uplc-Qtof-Ms Fingerprinting Combined With Chemometrics To Assess The Solvent Extraction Efficiency, Phytochemical Variation, And Antioxidant Activities Of Beta Vulgaris L., Junyi Wang, Guddadarangavvanahally Krishnareddy Jayaprakasha, Bhimanagouda S. Patil
Journal of Food and Drug Analysis
Accurate assays of plant antioxidants and other phytochemicals require efficient extraction conditions and enable rigorous assessments of crop varieties and production systems. This study assessed the extraction of phytochemicals and antioxidants from conventionally or organically grown red and golden beets (Beta vulgaris L.), using twenty solvent (S1eS20) mixtures containing water, methanol, and ethanol alone or with acids (ascorbic, formic, acetic). Red beetroot extracted with methanol with or without acid had the highest betanin content (2791.0 mg/g and 8222.3 mg/g of fresh weight [FW], respectively) and golden beetroot extracted with methanol/ascorbic acid/water had the highest vulgaxanthin I (193.7 mg/g and 15.0 …
Food Preservative Sorbic Acid Deregulates Hepatic Fatty Acid Metabolism,
2020
Graduate Institute and Department of Physiology, College of Medicine, National Taiwan University, Taipei, Taiwan
Food Preservative Sorbic Acid Deregulates Hepatic Fatty Acid Metabolism, Chia-Hui Chen, Sin-Ni Ho, Po-An Hu, Yu Ru Kou, Tzong-Shyuan Lee
Journal of Food and Drug Analysis
Sorbic acid (SA) is one of the most commonly used food preservatives worldwide. Despite SA having no hepatotoxicity at legal dosages, its effect on hepatic lipid metabolism is still unclear. We investigated the effect of SA on hepatic lipid metabolism and its mechanism of action in C57BL/6 mice. Daily treatment with SA (1 g/kg in diet) for 4 weeks did not alter the body weight, organ weight, and blood lipids in mice. However, hepatic lipid accumulation, particularly that of triglycerides, fatty acids, and glycerol, but not cholesteryl ester and free cholesterol, was increased with SA treatment. Mechanistically, SA decreased the …
The Potential Role Of Phenolic Compounds On Modulating Gut Microbiota In Obesity,
2020
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
The Potential Role Of Phenolic Compounds On Modulating Gut Microbiota In Obesity, Gow-Chin Yen, Hsin-Lin Cheng, Li-Yu Lin, Shiuan-Chih Chen, Chin Lin Hsu
Journal of Food and Drug Analysis
Obesity is a rising public health issue and challenge which is tightly correlated with socio-economic development paralleled with increased energy intake and sedentary behavior that subsequently cause adipose tissue accumulation. Physiological and metabolic status changes during obesity development have been suggested with low grade inflammation of gastrointestinal tract. The gut microbiota plays an essential role in regulating whole body energy metabolism and also lipid accumulation, and immunity of host. However, the detail mechanism of which the gut microbiota composition influence obesity development in humans still need deeper investigation owing to the complex pathophysiology of such disease. Recently, the consumption of …
Transcriptome Studies On The Toxicity Of Silica Nanoparticles,
2020
University of South Florida
Transcriptome Studies On The Toxicity Of Silica Nanoparticles, Shih-Yi Hsu
USF Tampa Graduate Theses and Dissertations
Nanotechnology enables more precise harmony in health condition via reducing the dosage amount, improving the delivery of hydrophobic drugs, more specific targeting to the cancerous sites, and so on. Nevertheless, issues regarding the toxicity of nanotechnology have begun to call for attention several decades later after the innovation of nanotechnology. Tools about risk management of nanotechnology have been developed, but recently not much evidence recognizes the toxicity of nanoparticles (NPs) except for some animal studies, which demonstrated organ damage after the exposure to NPs. Toxicogenomic approach refers to the method utilizing gene expression to evaluate the chemical toxicity. Databases as …
Development Of A Pharmaceutical Tablet Authentication System Using Spectroscopic Techniques In Combination With Multivariate Chemometric Methods,
2020
Duquesne University
Development Of A Pharmaceutical Tablet Authentication System Using Spectroscopic Techniques In Combination With Multivariate Chemometric Methods, Md Nayeem Hossain
Electronic Theses and Dissertations
The spread of falsified drugs is increasing worldwide. Currently, 10%-30% of drugs in the world are falsified. Unfortunately, the supply chain system of developing countries (from manufacturers to customers) is not well monitored and suffers the highest rates of fraudulent activities. Also, the rise of product procurement from the internet increases the chance of American consumers' exposure to poor quality drugs. To combat this horrendous activity, surveillance of pharmaceutical materials is required in the supply chain system. Spectroscopic techniques (e.g., Near-Infrared and Raman spectroscopies) can be a potential solution to authenticate samples in different locations; they are non-destructive, safe, rapid, …
Study Of Pharmaceutical Tablets Using Raman Mapping,
2020
University of Mississippi
Study Of Pharmaceutical Tablets Using Raman Mapping, Kyle Joseph Pauly
Honors Theses
Covalent bonds are the strongest type of bonds holding molecules together. Based on the pattern of bonding of the molecule, the atoms associated with the bond will vibrate at a specific frequency. Utilizing vibrational spectroscopy, such as Raman spectroscopy, these unique vibrational frequencies can be used to detect the presence of analytes over a selected area. Furthermore, the intensities of the vibrational modes can be tracked to comparatively quantify the concentration of analytes at various locations. This is a method of great importance due to its ability to compare pharmaceutical tablets synthesized with different techniques. Here, the presence and concentration …
Long-Distance Phasing Of A Tentative "Enhancer" Single-Nucleotide Polymorphism With Yp2d6 Star Allele Definitions,
2020
Children's Mercy Hospital
Long-Distance Phasing Of A Tentative "Enhancer" Single-Nucleotide Polymorphism With Yp2d6 Star Allele Definitions, Erin C. Boone, Wendy Y. Wang, R Gaedigk, Mariana Cherner, Anick Bérard, J Steven Leeder, Neil A. Miller, Andrea Gaedigk
Manuscripts, Articles, Book Chapters and Other Papers
Background: The CYP2D6 gene locus has been extensively studied over decades, yet a portion of variability in CYP2D6 activity cannot be explained by known sequence variations within the gene, copy number variation, or structural rearrangements. It was proposed that rs5758550, located 116 kb downstream of the CYP2D6 gene locus, increases gene expression and thus contributes to variability in CYP2D6 activity. This finding has, however, not been validated. The purpose of the study was to address a major technological barrier, i.e., experimentally linking rs5758550, also referred to as the "enhancer" single-nucleotide polymorphism (SNP), to CYP2D6 haplotypes >100 kb away. To overcome …
Design And Synthesis Of Novel Analogs As Potential Antitubercular Agents,
2020
University of Mississippi
Design And Synthesis Of Novel Analogs As Potential Antitubercular Agents, Peggy Mccluggage
Honors Theses
Tuberculosis (TB) is an infectious, airborne disease which primarily infects the lungs. One-third of the world’s population is currently estimated to be infected with Mycobacterium tuberculosis (Mtb), the causative agent for TB [1]. Current treatment for this disease requires at least six months of taking multiple antibiotics with undesirable side effects [2]. Difficulty in complying to this regimen as well as the prevalence of HIV/AIDS has led to antimicrobial resistance seen in Mtb. In order to combat the Multi-Drug Resistant and Extensively-Drug Resistant strains of the disease-causing bacteria, preventative care and novel antibiotics are urgently needed [3]. The purpose for …
Therapeutic Drug Monitoring Of Apixaban Using Chromogenic Kits,
2020
East Tennessee State University
Therapeutic Drug Monitoring Of Apixaban Using Chromogenic Kits, Brooke Vogel
Undergraduate Honors Theses
Apixaban is a novel oral anticoagulant that prevents clotting by directly inhibiting Factor Xa in the coagulation cascade. Due to its different pharmacokinetics, previous standards for testing anticoagulant concentrations are ineffective at measuring apixaban. In this study, Hyphen Biomed Biophen Direct Xa Inhibitor and Biophen Heparin chromogenic kits from Aniara Diagnostica were used along with a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer to see if either of these kits provide acceptable precision and accuracy for the quantification of apixaban in plasma samples, as well as if there is a significant difference in these two kits at varying concentrations of apixaban. …
Antidepressants Versus Music Therapy: Which Treatment Is Better For Treating Depression In Parkinson's Disease?,
2020
Virginia Commonwealth University
Antidepressants Versus Music Therapy: Which Treatment Is Better For Treating Depression In Parkinson's Disease?, Emaan Dawood
Virginias Collegiate Honors Council Conference
Music and medicine are merging to treat depression in Parkinson’s disease (PD). Antidepressants, the current standard, demonstrate mixed effects in clinical trials, warranting other treatment options. Here, I compare music therapy to antidepressants in the treatment of depression in PD via literature review. Studies of depressed PD patients undergoing music therapy and/or antidepressants were reviewed with attention to variables including depression (on a patient-reported outcome) that were measured pre- and post- therapy. In the music studies, improvements were seen in depression. Methodological inconsistencies across these studies, a paucity of studies, and small sample sizes, however, necessitate more research to reach …
Orally Administered Nanoscale Drug Delivery System For Colorectal Cancer,
2020
University of South Florida
Orally Administered Nanoscale Drug Delivery System For Colorectal Cancer, Bhavya Shah
USF Tampa Graduate Theses and Dissertations
Mithramycin-A (Mit-A) is a US Food and Drug Administration (FDA) approved chemotherapeutic agent that is used for the treatment of testicular cancer. According to previous studies, Mit-A has shown promising results in targeting colorectal cancer (CRC) and cancer stem cells (CSCs) which are responsible for a highly invasive and metastatic forms of CRC. The use of Mit-A as chemotherapy is limited in CRC due to its toxicity. To minimize its toxicity, we have developed an orally delivered nanoscale drug delivery system by which Mit-A can be delivered using biocompatible and biodegradable materials. Mit-A nanoparticles were prepared using Poly(lactic-co-glycolic acid) as …
