Stability-Indicating Spectrofluorimetric Method With Enhanced Sensitivity For Determination Of Vancomycin Hydrochloride In Pharmaceuticals And Spiked Human Plasma: Application To Degradation Kinetics,
2020
Taiwan Food and Drug Administration
Stability-Indicating Spectrofluorimetric Method With Enhanced Sensitivity For Determination Of Vancomycin Hydrochloride In Pharmaceuticals And Spiked Human Plasma: Application To Degradation Kinetics, El-Din M.K. Sharaf, F. Ibrahim, A.K. El-Deen, K. Shimizu
Journal of Food and Drug Analysis
Based on investigating the relative fluorescence intensity of vancomycin hydrochloride (VCM) in methanol, a simple, highly sensitive, time-saving and specific spectrofluorimetric method was developed and validated. VCM fluorescence was measured at 335 nm when excited at 268 nm. Excellent linearity is obeyed in the concentration range 1–100 ng/mL with a detection limit of 5.94 pg/mL, a quantitation limit of 18.03 pg/mL and a very good correlation coefficient (r = 0.9999). Our method was applied to analyze VCM in pharmaceuticals as well as spiked human plasma. Moreover, VCM stability was studied when exposed to various degradation conditions such as oxidative, alkaline …
Cytotoxic Protobassic Acid Saponins From The Kernels Of Palaquium Formosanum,
2020
Taiwan Food and Drug Administration
Cytotoxic Protobassic Acid Saponins From The Kernels Of Palaquium Formosanum, H.-Y. Chen, J.-F. Liu, S.-F. Tsai, Y.-L. Lin, S.-S. Lee
Journal of Food and Drug Analysis
Bioassay guided fractionation and separation of the EtOH extract of the kernels of Palaquium formosanum against PC-3 cells via Sephadex LH-20 and reverse phase C-18 columns led to the isolation of 13 protobassic saponins. One of these saponins is new and was characterized as 3‴-O-rhamnopyranosyl-arganin C, a bisdesmoside of 16α-hydroxyprotobassic acid at the C-3 and C-28 positions. The structures of these compounds were determined on the basis of 1D NMR ( 1 H, 13 C), 2D NMR ( 1 H– 1 H COSY, HSQC, HMBC, and NOESY), and selectively excited 1D TOCSY spectroscopic analyses and MS data, and comparison with …
Gallic Acid Inhibits Bladder Cancer Cell Proliferation And Migration Via Regulating Fatty Acid Synthase (Fas),
2020
Taiwan Food and Drug Administration
Gallic Acid Inhibits Bladder Cancer Cell Proliferation And Migration Via Regulating Fatty Acid Synthase (Fas), C.-C. Liao, S.-C. Chen, H.-P. Huang, C.-J. Wang
Journal of Food and Drug Analysis
Bladder cancer is known as the world's ninth most prevalent cancer in 2012. New cytotoxic drugs have created considerable progress in the treatment. Gallic acid (GA) has been shown to inhibit carcinogenesis in animal models and various cancer cell lines. The aim of the present study was to evaluate the effect of GA on proliferation and migration inhibition of a bladder cancer cell line. The results showed that GA inhibited fatty acid synthase (FAS) activity and increased ER alpha level of TSGH-8301 bladder cancer cell. GA regulated the cell proliferation via the PI3K/AKT and MAPK/ERK pathway. Immunoprecipitation assay demonstrated that …
An Investigation Of The Olive Phenols Activity As A Natural Medicine,
2020
Taiwan Food and Drug Administration
An Investigation Of The Olive Phenols Activity As A Natural Medicine, F. Gökalp
Journal of Food and Drug Analysis
The natural antioxidants of olive oil have phenolic structure and their activities are related to the formation of stable derivatives. In this study, the single components of the phenolic fraction of olive oil (1,4-hydroquinone, Semiquinone and 1,4-benzoquinone) have been studied as theoretical by using DFT (Density functional Theory). The behaviors of phenolic compounds of olive against to the alkyl peroxy radicals were investigated. Our data show that 1,4-benzoquinone is the best electron transfer agent in primary metabolic processes to human life. The frontier orbital gap, namely HOMO (highest occupied molecular orbital)–LUMO (lowest unoccupied molecular orbital) gap is the smallest for …
A Simple And Robust Quantitative Analysis Of Retinol And Retinyl Palmitate Using A Liquid Chromatographic Isocratic Method,
2020
Taiwan Food and Drug Administration
A Simple And Robust Quantitative Analysis Of Retinol And Retinyl Palmitate Using A Liquid Chromatographic Isocratic Method, S. Yokota, S. Oshio
Journal of Food and Drug Analysis
Vitamin A is a vital nutritional substances that regulates biological activities including development, but is also associated with disease onset. The extent of vitamin A intake influences the retinoid content in the liver, the most important organ for the storage of vitamin A. Measurement of endogenous retinoid in biological samples is important to understand retinoid homeostasis. Here we present a reliable, highly sensitive, and robust method for the quantification of retinol and retinyl palmitate using a reverse-phase HPLC/UV isocratic method. We determined the impact of chronic dietary vitamin A on retinoid levels in livers of mice fed an AIN-93G semi-purified …
Antioxidant Activity And Calcium Binding Of Isomeric Hydroxybenzoates,
2020
Taiwan Food and Drug Administration
Antioxidant Activity And Calcium Binding Of Isomeric Hydroxybenzoates, Z. Zhao, M. Vavrusova, L.H. Skibsted
Journal of Food and Drug Analysis
The association constant for calcium binding to hydroxybenzoates in aqueous 0.16 M NaCl at 25 °C was found electrochemically to have the value K ass = 280 mol L −1 with ΔH o = −51 kJ mol −1 , ΔS o = −122 J mol −1 K −1 for the 2-isomer (salicylate), K ass = 7 mol L −1 with ΔH o = −39 kJ mol −1 , ΔS o = −116 J mol −1 K −1 for the 3-isomer, and K ass = 8 mol L −1 with ΔH o = −51 kJ mol −1 , ΔS o = …
Systematic Study On Active Compounds As Antibacterial And Antibiofilm Agent In Aging Onions,
2020
Taiwan Food and Drug Administration
Systematic Study On Active Compounds As Antibacterial And Antibiofilm Agent In Aging Onions, K. Sharma, N. Mahato, Y.R. Lee
Journal of Food and Drug Analysis
Quantitative investigation and systematic studies of quercetin, total phenolics, flavonoids, antioxidants, antibacterial and antibiofilm or antibiofouling properties of methanolic extracts of onions obtained from six different varieties have been carried out to explore their relative merits in terms of biological activities of fresh and aging onions. Total phenolic content in the extracts was examined spectrophotometrically using Folin–Ciocalteau's phenol reagent and total antioxidant activity was studied by FRAP and DPPH methods. In vitro antibacterial activity of the extracts was investigated on Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and Bacillus cereus) respectively, by using a modified Kirby–Bauer disc …
Compositional Analyses Of White, Brown And Germinated Forms Of Popular Malaysian Rice To Offer Insight Into The Growing Diet-Related Diseases,
2020
Taiwan Food and Drug Administration
Compositional Analyses Of White, Brown And Germinated Forms Of Popular Malaysian Rice To Offer Insight Into The Growing Diet-Related Diseases, B. Abubakar, H.M. Yakasai, N. Zawawi, M. Ismail
Journal of Food and Drug Analysis
Diet-related metabolic diseases, and especially obesity, are metabolic disorders with multifactorial aetiologies. Diet has been a cornerstone in both the aetiology and management of this metabolic disorders. Rice, a staple food for over half of the world's population, could be exploited as part of the solution to check this menace which has been skyrocketing in the last decade. The present study investigated nine forms of rice from three widely grown Malaysian rice cultivars for in vitro and in vivo (glycaemic index and load) properties that could translate clinically into a lower predisposition to diet-related diseases. The germinated brown forms of …
Effects Of Electrode Gap And Electric Current On Chlorine Generation Of Electrolyzed Deep Ocean Water,
2020
Taiwan Food and Drug Administration
Effects Of Electrode Gap And Electric Current On Chlorine Generation Of Electrolyzed Deep Ocean Water, G.-S.W. Hsu, S.-Y. Hsu
Journal of Food and Drug Analysis
Electrolyzed water is a sustainable disinfectant, which can comply with food safety regulations and is environmental friendly. A two-factor central composite design was adopted for studying the effects of electrode gap and electric current on chlorine generation efficiency of electrolyzed deep ocean water. Deep ocean water was electrolyzed in a glass electrolyzing cell equipped with platinum-plated titanium anode and cathode in a constant-current operation mode. Results showed that current density, chlorine concentration, and electrolyte temperature increased with electric current, while electric efficiency decreased with electric current and electrode gap. An electrode gap of less than 11.7 mm, and a low …
Characterization Of Key Aroma-Active Compounds In Lychee (Litchi Chinensis Sonn.),
2020
Taiwan Food and Drug Administration
Characterization Of Key Aroma-Active Compounds In Lychee (Litchi Chinensis Sonn.), S. Feng, M. Huang, J.H. Crane, Y. Wang
Journal of Food and Drug Analysis
Volatile compounds in ‘Sweetheart’ lychee were examined using gas chromatography-olfactometry/mass spectrometry (GC-O/MS). Solvent assisted flavor evaporation (SAFE) technique was used to identify the aroma-active compounds in lychee. Further characterization of the most important odorants in ‘Sweetheart’ lychee was achieved using aroma extract dilution analysis (AEDA). Thirty-one key aroma-active odorants were identified in the flavor dilution (FD) factor range of 2–1024. Methional (cooked potato) and geraniol (sweet, floral) exhibited the highest FD factors of 1024 and 512, respectively, these were followed by furaneol (sweet, caramel), nerol (floral, sweet), dimethyl trisulfide (DMTS) (preserved vegetable, sulfury), linalool (floral), (E,Z)-2,6 nonadienal (cucumber) and nerolidol …
Hptlc-Fld-Sers As A Facile And Reliable Screening Tool: Exemplarily Shown With Tyramine In Cheese,
2020
Taiwan Food and Drug Administration
Hptlc-Fld-Sers As A Facile And Reliable Screening Tool: Exemplarily Shown With Tyramine In Cheese, L. Wang, X.-M. Xu, Y.-S. Chen, J. Ren, Y.-T. Liu
Journal of Food and Drug Analysis
The serious cytotoxicity of tyramine attracted marked attention as it induced necrosis of human intestinal cells. This paper presented a novel and facile high performance thin-layer chromatography (HPTLC) method tailored for screening tyramine in cheese. Separation was performed on glass backed silica gel plates, using methanol/ethyl acetate/ammonia (6/4/1 v/v/v) as the mobile phase. Special efforts were focused on optimizing conditions (substrate preparation, laser wavelength, salt types and concentrations) of surface enhanced Raman spectroscopy (SERS) measurements directly on plates after derivatization, which enabled molecule-specific identification of targeted bands. In parallel, fluorescent densitometry (FLD) scanning at 380
Water-Soluble Polysaccharides From Ulva Intestinalis: Molecular Properties, Structural Elucidation And Immunomodulatory Activities,
2020
Taiwan Food and Drug Administration
Water-Soluble Polysaccharides From Ulva Intestinalis: Molecular Properties, Structural Elucidation And Immunomodulatory Activities, M. Tabarsa, S. You, E.H. Dabaghian, U. Surayot
Journal of Food and Drug Analysis
Water-soluble sulfated polysaccharides extracted from Ulva intestinalis and fractionated using DEAE Sepharose fast flow column to identify their molecular properties and macrophage cells stimulating activities. Crude and fractions (F 1 and F 2 ) were formed of neutral sugars (58.7–74.7%), sulfates (6.2–24.5%), uronic acids (4.9–5.9%) and proteins (3.2–10.4%). Different levels of sugar constituents including rhamnose (30.1–39.1%), glucose (39.0–48.4%), galactose (0.0–15.8%), xylose (8.5–11.3) and arabinose (0.0–5.1%). The molecular weight (M w ) of crude and fractionated polysaccharides ranged from 87.1 × 10 3 to 194.1 × 10 3 (g/mol). Crude polysaccharides were not toxic to RAW264.7 cells and fractions induced cell …
Relative Content Of Gallic Acid Over 5-Galloylquinic Acid As An Index For The Baking Intensity Of Oolong Teas,
2020
Taiwan Food and Drug Administration
Relative Content Of Gallic Acid Over 5-Galloylquinic Acid As An Index For The Baking Intensity Of Oolong Teas, M.M.-C. Wang, Y. Yeh, Y.-E. Shih, J.T.-C. Tzen
Journal of Food and Drug Analysis
Phenolic compounds in a series of old oolong teas prepared by baking annually were monitored and compared. The results showed that the relative content of gallic acid over 5-galloylquinic acid was subsequently elevated during this preparatory process. To reveal the effect was mainly resulted from baking or aging, two sets of oolong teas were collected and examined; one set was generated from fresh oolong tea via continually daily baking and the other set was composed of aged oolong teas with no or light baking in the storage period. The relative content of gallic acid over 5-galloylquinic acid was observed to …
Production Of Antioxidant And Ace-Inhibitory Peptides From Kluyveromyces Marxianus Protein Hydrolysates: Purification And Molecular Docking,
2020
Taiwan Food and Drug Administration
Production Of Antioxidant And Ace-Inhibitory Peptides From Kluyveromyces Marxianus Protein Hydrolysates: Purification And Molecular Docking, M. Mirzaei, S. Mirdamadi, M.R. Ehsani, M. Aminlari
Journal of Food and Drug Analysis
Kluyveromyces marxianus protein hydrolysates were prepared by two different sonicated-enzymatic (trypsin and chymotrypsin) hydrolysis treatments to obtain antioxidant and ACE-inhibitory peptides. Trypsin and chymotrypsin hydrolysates obtained by 5 h, exhibited the highest antioxidant and ACE-inhibitory activities. After fractionation using ultrafiltration and reverse phase high performance liquid chromatography (RP-HPLC) techniques, two new peptides were identified. One fragment (LL-9, MW = 1180 Da) with the amino acid sequence of Leu-Pro-Glu-Ser-Val-His-Leu-Asp-Lys showed significant ACE inhibitory activity (IC 50 = 22.88 μM) while another peptide fragment (VL-9, MW = 1118 Da) with the amino acid sequence of Val-Leu-Ser-Thr-Ser-Phe-Pro-Pro-Lys showed the highest antioxidant and ACE …
Exploration Of Wedelia Chinensis Leaf-Assisted Silver Nanoparticles For Antioxidant, Antibacterial And In Vitro Cytotoxic Applications,
2020
Taiwan Food and Drug Administration
Exploration Of Wedelia Chinensis Leaf-Assisted Silver Nanoparticles For Antioxidant, Antibacterial And In Vitro Cytotoxic Applications, Das M. Paul, Livingstone J. Rebecca, P. Veluswamy, J. Das
Journal of Food and Drug Analysis
Green synthetic route of silver nanoparticles (AgNPs) has already been proved to be an advantageous over other physico-chemical approaches due to its simplicity, cost effectiveness, ecofriendly and nontoxicity. In this finding, aqueous Wedelia chinensis leaf extract (WLE) mediated synthesis of AgNPs was approached. Surface plasmon resonance (SPR) band at 408 nm preliminary indicated the formation of AgNPs, while TEM and XRD characterization confirmed the formation of spherically shaped and crystalline AgNPs with an average size of 31.68 nm, respectively. The plausible biomolecules in the aqueous leaf extract responsible for the reduction and stabilization of AgNPs were identified by FTIR analysis …
Characteristics Of Fucose-Containing Polysaccharides From Submerged Fermentation Of Agaricus Blazei Murill,
2020
Taiwan Food and Drug Administration
Characteristics Of Fucose-Containing Polysaccharides From Submerged Fermentation Of Agaricus Blazei Murill, H.-T. Wang, L.-C. Yang, H.-C. Yu, M.-L. Chen, H.-J. Wang, T.-J. Lu
Journal of Food and Drug Analysis
Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. We obtained the polysaccharides through a cell-based activity-guided strategy, and used carbohydrate recognition monoclonal antibodies based Enzyme-Linked Immuno Sorbent Assay (ELISA) along with methylation and NMR analyses to investigate the structural characteristics of the polysaccharides. The polysaccharides had Mw of 3.5 × 10 5 Da. The major sugars were L-fucose, L-arabinose, D-galactose, D-xylose, and D-galacturonic acid in the molar ratio of 6.4, 15.5, 28.5, 14.7, and 25.0% with a small amount …
Determination Of Total Mercury In Seafood By Ion-Selective Electrodes Based On A Thiol Functionalized Ionic Liquid,
2020
Taiwan Food and Drug Administration
Determination Of Total Mercury In Seafood By Ion-Selective Electrodes Based On A Thiol Functionalized Ionic Liquid, J. Miao, X. Wang, Y. Fan, J. Li, L. Zhang, G. Hu, C. He, C. Jin
Journal of Food and Drug Analysis
A mercury(II) ion-selective electrode with an ionic liquid (IL), 1-methyl-2-butylthioimidazolium bis(trifluoromethanesulphonyl)imide ([C 1 C 4 Sim]NTf 2 ) as active material was constructed. Parameters affecting the performance of the electrodes such as the dosages of the IL and carbon nanotubes and the aqueous pH values were investigated. Experimental results indicated that the optimal composition of the electrode filling material was 47.6% [C 1 C 4 Sim]NTf 2 , 47.6% tetrabutylphosphonium bis(trifluoromethanesulphonyl)imide (TBPNTf 2 ) and 4.8% carboxylic multi-walled carbon nanotubes (MWCNTs-COOH). Under the selected conditions, the proposed electrodes showed a good linear response in the concentration range of 10 −10 …
Analysis Of Veterinary Drug Residue Monitoring Results For Commercial Livestock Products In Taiwan Between 2011 And 2015,
2020
Taiwan Food and Drug Administration
Analysis Of Veterinary Drug Residue Monitoring Results For Commercial Livestock Products In Taiwan Between 2011 And 2015, H.-C. Lee, C.-M. Chen, J.-T. Wei, H.-Y. Chiu
Journal of Food and Drug Analysis
Antibiotics have been widely used in the treatment of livestock diseases. However, the emergence of issues related to drug resistance prompted governments to enact a series of laws regulating the use of antibiotics in livestock. Following control of the problem of drug resistant bacteria, public attention has shifted to the recurring incidence of human health and safety issues caused by residual veterinary drugs in livestock products. To guarantee the safety and hygiene of meat, milk, and eggs from food-producing animals, governments and relevant agencies established laws and regulations for the use of veterinary drugs. It is, therefore, necessary to monitor …
A Validated Capillary Electrophoretic Method For The Determination Of Indacaterol And Its Application To A Pharmaceutical Preparation,
2020
Taiwan Food and Drug Administration
A Validated Capillary Electrophoretic Method For The Determination Of Indacaterol And Its Application To A Pharmaceutical Preparation, T. Güray, M. Tunçel, U.D. Uysal
Journal of Food and Drug Analysis
Indacaterol is a new inhaled ultra-long acting β 2 -agonist. It has been recently approved in the European Union for the treatment of chronic obstructive pulmonary disease. This paper reports, for the first time, a method for the determination and validation of Indacaterol (IND) using an internal standard in capsules. Capillary electrophoretic separation was performed on an uncoated fused-silica capillary (50 cm effective length, 75 μm i.d.) and background electrolyte composed of 20 mmol L −1 of sodium tetraborate buffer, 15% (v/v) methanol (pH = 10.0) with the application of 20 kV of potential; 10 s at 5 × 10 …
Sialoglycoprotein Isolated From Eggs Of Carassius Auratus Promotes Fracture Healing In Osteoporotic Mice,
2020
Taiwan Food and Drug Administration
Sialoglycoprotein Isolated From Eggs Of Carassius Auratus Promotes Fracture Healing In Osteoporotic Mice, F. Wang, L. Han, X. Wang, Y. Li, Y. Zhu, J. Wang, C. Xue
Journal of Food and Drug Analysis
In this study, open tibial fracture surgery was performed on mice with ovariectomy induced osteoporosis to investigate the effect of a treatment with sialoglycoprotein isolated from Carassius auratus eggs (Ca-SGP) on fracture healing. Dynamic histological analysis showed that Ca-SGP promoted the generation of cartilage callus on day 5 post-surgery, then facilitated the transformation of the cartilage callus to bony callus on days 11 and 24 post-surgery, and enhanced the remodeling of bony callus on 35 day post-surgery. Moreover, Ca-SGP significantly decreased the secretion of TNF-α and IL-1β in serum on day 5 post-surgery, thus inhibiting the negative spread of the …
