Concentrations Of Perfluoroalkyl Substances In Foods And The Dietary Exposure Among Taiwan General Population And Pregnant Women,
2020
Taiwan Food and Drug Administration
Concentrations Of Perfluoroalkyl Substances In Foods And The Dietary Exposure Among Taiwan General Population And Pregnant Women, W.-L. Chen, F.-Y. Bai, Y.-C. Chang, P.-C. Chen, C.-Y. Chen
Journal of Food and Drug Analysis
This study quantified five perfluorocarboxylic acids (PFCAs) and two perfluorosulfonic acids in cereals, meats, seafood, eggs, pork liver, and milk in Taiwan using ultra-performance liquid chromatography-tandem mass spectrometry and evaluated the dietary exposure of the general population and pregnant women using per capita consumption and a questionnaire, respectively. Perfluorooctanoic acid (PFOA) and PFCAs of 10–12 carbons were found in almost all of the samples in considerable concentrations in rice and pork liver, reaching as high as 283 ng/g (PFOA in pork liver); the levels are two to three orders of magnitude higher than previous reports. Perfluorooctane sulfonate (PFOS), the most …
Application Of Thermal Stability Difference To Remove Flammutoxin In Fungal Immunomodulatory Protein, Fip-Fve, Extract From Flammulina Velutipes,
2020
Taiwan Food and Drug Administration
Application Of Thermal Stability Difference To Remove Flammutoxin In Fungal Immunomodulatory Protein, Fip-Fve, Extract From Flammulina Velutipes, C.-H. Tung, C.-C. Lin, H.-J. Wang, S.-F. Chen, F. Sheu, T.-J. Lu
Journal of Food and Drug Analysis
Fungal immunomodulatory protein (FIP-fve) is a potential functional food ingredient. However, undesirable component flammutoxin (FTX) would occur in the extracted fraction of FIP-fve. In this paper, an application of heating processing instead of the intensive separation process was employed in fractionation of FIP-fve, meanwhile, exclusion of FTX was reached. Contents of FIP-fve and FTX were monitored by HPLC-UV-ESI-MS. Both FIP-fve and FTX had higher thermal stability in a lower concentration solution. Cold water could effectively extract FIP-fve and FTX from fresh mushroom without acetic acid and disulfide-bond breaking agent β-mercaptoethanol commonly used in biochemical studies. Heating cold water extract contained …
Combination Of On-Line Desalting And Hplc-Uv-Esi-Ms For Simultaneous Detection And Identification Of Fip-Fve And Flammutoxin In Flammulina Velutipes,
2020
Taiwan Food and Drug Administration
Combination Of On-Line Desalting And Hplc-Uv-Esi-Ms For Simultaneous Detection And Identification Of Fip-Fve And Flammutoxin In Flammulina Velutipes, C.-H. Tung, C.-C. Lin, C.-C. Tung, S.-F. Chen, F. Sheu, T.-J. Lu
Journal of Food and Drug Analysis
A rapid analytical approach, on-line desalting HPLC-UV-ESI-MS method, for the analysis of FIP-fve and flammutoxin (FTX), two important bioactive proteins in the fruiting bodies of Flammulina velutipes, was developed. In this study, a highly efficient desalting method is provided using molecular weight cut-off centrifugal filtration and on-line desalting. Sample preparation followed by an on-line desalting HPLC-UV-ESI-MS system was employed for simultaneous desalting and detection and identification of FIP-fve and FTX. Results indicated that using trifluoroacetic acid as a modifier on a C18 reversed-phase column renders effective separation. ESI-MS revealed that the apparent molecular masses of FIP-fve and FTX were 12,749.1 …
A Feasible And Practical 1h Nmr Analytical Method For The Quality Control And Quantification Of Bioactive Principles In Lycii Fructus,
2020
Taiwan Food and Drug Administration
A Feasible And Practical 1h Nmr Analytical Method For The Quality Control And Quantification Of Bioactive Principles In Lycii Fructus, L.-Y. Hsieh, H.-H. Chan, P.-C. Kuo, H.-Y. Hung, Y.-C. Li, C.-L. Kuo, Y. Peng, Z.-Z. Zhao, D.-H. Kuo, I.-W. Sun, T.-S. Wu
Journal of Food and Drug Analysis
Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid …
Comparative Study Of Two Different Chromatographic Approaches For Quantitation Of Hydrocortisone Acetate And Pramoxine Hydrochloride In Presence Of Their Impurities,
2020
Taiwan Food and Drug Administration
Comparative Study Of Two Different Chromatographic Approaches For Quantitation Of Hydrocortisone Acetate And Pramoxine Hydrochloride In Presence Of Their Impurities, F. Ibrahim, A.K. El-Deen, K. Shimizu
Journal of Food and Drug Analysis
In the present study, we compare the performance of two reversed-phase liquid chromatographic approaches using different eluents either conventional hydro-organic eluent or micellar one for simultaneous estimation of hydrocortisone acetate and pramoxine hydrochloride in presence of their degradants and process-related impurities; hydrocortisone and 4-butoxyphenol, respectively. For conventional reversed-phase liquid chromatography (RPLC), separation of the studied compounds was completed on an Inertsil ODS 3-C18 column (150 mm × 4.6 mm, 5 μm particle size) with a mobile phase consists of 50 mM phosphate buffer (pH 5.0): acetonitrile (50: 50, v/v). For micellar liquid chromatography (MLC), an Eclipse XDB-C8 column (150 mm …
Improvement Of Nutritional Components And In Vitro Antioxidative Properties Of Soy-Powder Yogurts Using Lactobacillus Plantarum,
2020
Taiwan Food and Drug Administration
Improvement Of Nutritional Components And In Vitro Antioxidative Properties Of Soy-Powder Yogurts Using Lactobacillus Plantarum, J.H. Lee, C.E. Hwang, E.J. Cho, Y.H. Song, S.C. Kim, K.M. Cho
Journal of Food and Drug Analysis
This research was the first to demonstrate changes in nutritional compositions (isoflavone and CLA) from the 50% methanol extracts of soy-powder milk (SPM) and soy-powder yogurt (SPY) through fermentation using Lactobacillus plantarum S48 and P1201 strains. The radical scavenging activities and protective effects against oxidative stress in LLC-PK1 cells were also investigated. The average physicochemical characteristics including acidity and viable cell number as well as β-glucosidase activity increased with 0.2 → 0.7%, 7.5 → 9.8 log cfu/mL, and 0.0 3 → 1.75 U/g in SPYs. Total average isoflavones were considerably reduced (3180.3 → 2018.3 μg/g) with the increase of aglycone …
Synergic Effect Of Curcumin And Its Structural Analogue (Monoacetylcurcumin) On Anti-Influenza Virus Infection,
2020
Taiwan Food and Drug Administration
Synergic Effect Of Curcumin And Its Structural Analogue (Monoacetylcurcumin) On Anti-Influenza Virus Infection, S.M. Richart, Y.-L. Li, Y. Mizushina, Y.-Y. Chang, T.-Y. Chung, G.-H. Chen, J.T.-C. Tzen, K.-S. Shia, W.-L. Hsu
Journal of Food and Drug Analysis
Curcumin (Cur), a polyphenolic compound extracted from spice and common food colourant turmeric, contains versatile bio-activities. Monoacetylcurcumin (MAC), a structural analogue of Cur, differs from Cur by acetyl modification, but retains enone groups. Comparative analysis revealed MAC effectively inhibited influenza virus infection (IAV) to a similar extent as, if not superior to, curcumin. Both compounds mildly reduced viral NA activity. Surprisingly, unlike Cur, the MAC inhibition of IAV did not occur through the blocking of HA activity. However, MAC strongly dampened Akt phosphorylation, the prerequisite signalling for efficient IAV propagation. A much stronger inhibition effect on IAV infection was observed …
Resveratrol Protects Muscle Cells Against Palmitate-Induced Cellular Senescence And Insulin Resistance Through Ameliorating Autophagic Flux,
2020
Taiwan Food and Drug Administration
Resveratrol Protects Muscle Cells Against Palmitate-Induced Cellular Senescence And Insulin Resistance Through Ameliorating Autophagic Flux, Y.-C. Chang, H.-W. Liu, Y.-T. Chen, Y.-A. Chen, Y.-J. Chen, S.-J. Chang
Journal of Food and Drug Analysis
Skeletal muscle, a highly metabolic tissue, is particularly vulnerable to increased levels of saturated free fatty acids (FFAs). The role of autophagy in saturated FFAs-induced cellular senescence and insulin resistance in skeletal muscle remains unclear. Therefore, the present study was aimed to explore autophagic flux in cellular senescence and insulin resistance induced by palmitate in muscle cells, and whether resveratrol limited these responses. Our results showed that palmitate induced cellular senescence in both myoblasts and myotubes. In addition, palmitate delayed differentiation in myoblasts and inhibited expression of insulin-stimulated p-AKTSer473 in myotubes. The accumulations of autophagosome assessed by tandem fluorescent-tagged LC3 …
Effects Of Vitamin B-6 Supplementation On Oxidative Stress And Inflammatory Response In Neonatal Rats Receiving Hyperoxia Therapy,
2020
Taiwan Food and Drug Administration
Effects Of Vitamin B-6 Supplementation On Oxidative Stress And Inflammatory Response In Neonatal Rats Receiving Hyperoxia Therapy, M.-S. Lee, T.-C. Su, Y.-C. Huang, R.C. Yang, J.-K. Kao, C.-H. Lee, J.-J. Tseng, C.-S. Hsu, C.-L. Hsu
Journal of Food and Drug Analysis
Hyperoxia is often used in the treatment of neonates. However, protracted use of hyperoxia leads to significant morbidity. The purpose of this study was to evaluate the effects of vitamin B-6 supplementation on oxidative stress and inflammatory responses in neonatal rats undergoing hyperoxia therapy. The study consisted of 2 parts: a survival study and a vitamin B-6 efficacy study for 16 days. Neonatal rats were randomly divided into either the control group, B-6 group (subcutaneously injected with 90 mg/kg/d of pyridoxal 5′-phosphate [PLP]), O2 group (treated with 85% oxygen), or O2 + B-6 group (simultaneously treated with 85% oxygen and …
Quantitative And Qualitative Analyses Of Cytotoxic Triterpenoids In The Rhizomes Of Anemone Raddeana Using Hplc And Hplc-Esi-Q/Tof-Ms,
2020
Taiwan Food and Drug Administration
Quantitative And Qualitative Analyses Of Cytotoxic Triterpenoids In The Rhizomes Of Anemone Raddeana Using Hplc And Hplc-Esi-Q/Tof-Ms, Y. Zhao, X. Zhang, C. Lv, Y. Yu, Y. Zhang, J. Lu
Journal of Food and Drug Analysis
Anemone raddeana Regel, a Traditional Chinese Medicine, has been demonstrated to possess cytotoxicity and anti-inflammatory activities. The purpose of this study is to establish analytical methods to identify and quantify the major active constituents in Anemone raddeana. A high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-Q/TOF-MS) was used to identify the components in the title plant material. To quantify the major components, a HPLC-UV method was developed and validated. The results showed that 37 compounds were identified based on the MS data and retention times. The contents of eight main bioactive compounds were determined by …
Investigation Of Aflatoxins Contamination In Herbal Materia Medica In A Taiwan Pharmaceutical Factory,
2020
Taiwan Food and Drug Administration
Investigation Of Aflatoxins Contamination In Herbal Materia Medica In A Taiwan Pharmaceutical Factory, M.-Y. Chien, C.-M. Yang, C.-M. Huang, C.-H. Chen
Journal of Food and Drug Analysis
During the years 2005–2016, a total of 1067 samples for 24 types of herbal materia medica were investigated for the presence of aflatoxins (AFs) using immunoaffinity column cleanup and HPLC-coupled to a fluorescence detector after post-column derivatization. AFs were detected in 373 (35%) out of the total samples. Among them, Platycladi Semen (65% for total AFs and 79% for AFB1), Corydalis Rhizoma (53% for total AFs and 32% for AFB1), Corni Fructus (3% for total AFs), Coicis Semen (3% for total AFs and AFB1), Nelumbinis Semen (6% for total AFs and 9% for AFB1), Arecae Semen (18% for AFB1), Polygalae …
Characterization Of Titanium Dioxide And Zinc Oxide Nanoparticles In Sunscreen Powder By Comparing Different Measurement Methods,
2020
Taiwan Food and Drug Administration
Characterization Of Titanium Dioxide And Zinc Oxide Nanoparticles In Sunscreen Powder By Comparing Different Measurement Methods, P.J. Lu, S.W. Fang, W.L. Cheng, S.C. Huang, M.C. Huang, H.F. Cheng
Journal of Food and Drug Analysis
Numerous consumer products, such as cosmetics, contain nanoparticles (NPs) of titanium dioxide (TiO2) or zinc oxide (ZnO); however, this raises questions concerning the safety of such additives. Most of these products do not indicate whether the product includes NPs. In this study, we characterized metal oxide NPs according to size, shape, and composition as well as their aggregation/agglomeration characteristics. In order to comprehend quickly the characterization of metal oxide NPs, we employed single particle inductively coupled plasma (SP-ICPMS) to help quantify the size of metal oxide NPs; then, we use transmission electron microscopy (TEM) to corroborate the results. The crystal …
Flavonoids Luteolin And Quercetin Inhibit Rps19 And Contributes To Metastasis Of Cancer Cells Through C-Myc Reduction,
2020
Taiwan Food and Drug Administration
Flavonoids Luteolin And Quercetin Inhibit Rps19 And Contributes To Metastasis Of Cancer Cells Through C-Myc Reduction, K.-C. Chen, W.-H. Hsu, J.-Y. Ho, C.-W. Lin, C.-Y. Chu, C.C. Kandaswami, M.-T. Lee, C.-H. Cheng
Journal of Food and Drug Analysis
Flavonoids luteolin and quercetin can inhibit growth and metastasis of cancer cells. In our previous report, luteolin and quercetin was shown to block Akt/mTOR/c-Myc signaling. Here, we found luteolin and quercetin reduced protein level and transactivation activity of RPS19 in A431-III cells, which is isolated from parental A431 (A431-P) cell line. Further investigation the inhibitory mechanism of luteolin and quercetin on RPS19, we found c-Myc binding sites on RPS19 promoter. The Akt inhibitor LY294002, mTOR inhibitor rapamycin and c-Myc inhibitor 10058-F4 significantly suppressed RPS19 expression and transactivation activities. Overexpression and knockdown of c-Myc in cancer cells show RPS19 expression was …
Production Of Functional Peptides With Inhibition Ability Against Angiotensin I-Converting Enzyme Using P. Pastoris Expression System,
2020
Taiwan Food and Drug Administration
Production Of Functional Peptides With Inhibition Ability Against Angiotensin I-Converting Enzyme Using P. Pastoris Expression System, H.-M. Tai, C.-C. Li, C.-Y. Hung, L.-J. Yin
Journal of Food and Drug Analysis
To obtain the angiotension-I converting enzyme inhibitor (ACEI), a fusion ACEI polypeptide encoded with 8 DNA sequences of GPL, GPM, IKW, IVY, IRPVQ, IWHHT, IYPRY and IAPG, which were selected and designed and cloned into pGAPZαC and then transformed into Pichia pastoris SMD1168H. After 3 days induction, the fraction with highest ACEI activity was expressed and purified using a Ni Sepharose™ 6 Fast Flow. The IC50 of recombinant ACEI polypeptide was 88.2 μM. A 128-fold increase of ACEI activity (0.69 μM) was obtained after pepsin digestion, which was equivalent to 0.022 μM of captopril. Reverse phase HPLC indicated all the …
Comprehensive Quality Evaluation And Comparison Of Angelica Sinensis Radix And Angelica Acutiloba Radix By Integrated Metabolomics And Glycomics,
2020
Taiwan Food and Drug Administration
Comprehensive Quality Evaluation And Comparison Of Angelica Sinensis Radix And Angelica Acutiloba Radix By Integrated Metabolomics And Glycomics, S.-S. Zhou, J. Xu, C.-K. Tsang, K.-M. Yip, W.-P. Yeung, Z.-Z. Zhao, S. Zhu, H. Fushimi, H.-Y. Chang, H.-B. Chen
Journal of Food and Drug Analysis
Angelica radix (Danggui in Chinese) used in China and Japan is derived from two species of Angelica, namely Angelica sinensis and Angelica acutiloba, respectively. The differences in quality between A. sinensis radix (ASR) and A. acutiloba radix (AAR) should be therefore investigated to guide the medicinal and dietary applications of these two species. Secondary metabolites and carbohydrates have been demonstrated to be the two major kinds of bioactive components of Danggui. However, previously, quality comparison between ASR and AAR intensively concerned secondary metabolites but largely overlooked carbohydrates, thus failing to include or take into consideration an important aspect of the …
Benefaction Of Probiotics For Human Health: A Review,
2020
Taiwan Food and Drug Administration
Benefaction Of Probiotics For Human Health: A Review, Kerry R. George, J.K. Patra, S. Gouda, Y. Park, H.-S. Shin, G. Das
Journal of Food and Drug Analysis
Humans are a unique reservoir of heterogeneous and vivacious group of microbes, which together forms the human-microbiome superorganism. Human gut serves as a home to over 100–1000 microbial species, which primarily modulate the host internal environment and thereby, play a major role in host health. This spectacular symbiotic relationship has attracted extensive research in this field. More specifically, these organisms play key roles in defense function, eupepsia along with catabolism and anabolism, and impact brain-gut responses. The emergence of microbiota with resistance and tolerance to existing conventional drugs and antibiotics has decreased the drug efficacies. Furthermore, the modern biotechnology mediated …
Tetrahydrocurcumin Ameliorates Free Fatty Acid-Induced Hepatic Steatosis And Improves Insulin Resistance In Hepg2 Cells,
2020
Taiwan Food and Drug Administration
Tetrahydrocurcumin Ameliorates Free Fatty Acid-Induced Hepatic Steatosis And Improves Insulin Resistance In Hepg2 Cells, J.-W. Chen, Z.-L. Kong, M.-L. Tsai, C.-Y. Lo, C.-T. Ho, C.-S. Lai
Journal of Food and Drug Analysis
Elevated levels of free fatty acids (FFAs) in the liver, resulting from either increased lipolysis or imbalanced FFAs flux, is a key pathogenic factor of hepatic steatosis. This study was conducted to examine the therapeutic effect of tetrahydrocurcumin (THC), a naturally occurring curcuminoid and a metabolite of curcumin, on oleic acid (OA)-induced steatosis in human hepatocellular carcinoma cells and to elucidate the underlying mechanism. HepG2 cells were incubated with OA to induce steatosis, and then treated with various concentrations of THC. The results showed that THC treatment significantly decreased lipid accumulation in OA-treated HepG2 cells, possibly, by inhibiting the expression …
Pyrrole-Protein Adducts – A Biomarker Of Pyrrolizidine Alkaloid-Induced Hepatotoxicity,
2020
Taiwan Food and Drug Administration
Pyrrole-Protein Adducts – A Biomarker Of Pyrrolizidine Alkaloid-Induced Hepatotoxicity, J. Ma, Q. Xia, P.P. Fu, G. Lin
Journal of Food and Drug Analysis
Pyrrolizidine alkaloids (PAs) are phytotoxins identified in over 6000 plant species worldwide. Approximately 600 toxic PAs and PA N-oxides have been identified in about 3% flowering plants. PAs can cause toxicities in different organs particularly in the liver. The metabolic activation of PAs is catalyzed by hepatic cytochrome P450 and generates reactive pyrrolic metabolites that bind to cellular proteins to form pyrrole-protein adducts leading to PA-induced hepatotoxicity. The mechanisms that pyrrole-protein adducts induce toxicities have not been fully characterized. Methods for qualitative and quantitative detection of pyrrole-protein adducts have been developed and applied for the clinical diagnosis of PA exposure …
Recent Progress In Natural Dietary Non-Phenolic Bioactives On Cancers Metastasis,
2020
Taiwan Food and Drug Administration
Recent Progress In Natural Dietary Non-Phenolic Bioactives On Cancers Metastasis, G.-C. Yen, C.-M. Tsai, C.-C. Lu, C.-J. Weng
Journal of Food and Drug Analysis
From several decades ago to now, cancer continues to be the leading cause of death worldwide, and metastasis is the major cause of cancer-related deaths. For health benefits, there is a great desire to use non-chemical therapy such as nutraceutical supplementation to prevent pathology development. Over 10,000 different natural bioactives or phytochemicals have been known that possessing potential preventive or supplementary effects for various diseases including cancer. Previously, the in vitro and in vivo anti-invasive and anti-metastatic activities of phenolic acids, monophenol, polyphenol and their derivatives and flavonoids and their derivatives have been reviewed. However, a vast number of natural …
Simultaneous Analysis Of 23 Illegal Adulterated Aphrodisiac Chemical Ingredients In Health Foods And Chinese Traditional Patent Medicines By Ultrahigh Performance Liquid Chromatography Coupled With Quadrupole Time-Of-Flight Mass Spectrometry,
2020
Taiwan Food and Drug Administration
Simultaneous Analysis Of 23 Illegal Adulterated Aphrodisiac Chemical Ingredients In Health Foods And Chinese Traditional Patent Medicines By Ultrahigh Performance Liquid Chromatography Coupled With Quadrupole Time-Of-Flight Mass Spectrometry, X.-B. Wang, J. Zheng, J.-J. Li, H.-Y. Yu, Q.-Y. Li, L.-H. Xu, M.-J. Liu, R.-Q. Xian, Y.-E. Sun, B.-J. Liu
Journal of Food and Drug Analysis
This paper presents an application of ultra high-performance liquid-chromatography-quadrupole-TOF high resolution mass spectrometry (UHPLC-Q-TOF HRMS) for simultaneous analysis of 23 illegal adulterated aphrodisiac type chemical ingredients in health foods and Chinese Traditional Patent Medicines (CTPMs). The mass spectrometer was operated in Information Dependent Acquisition (IDA) mode, which provides crucial information for the elemental composition analysis, structure elucidation and quantitative analysis simultaneously. Quantitative analysis was performed using the peak areas of the precursor ions in the XICs. The method validation included assessment of selectivity, sensitivity, calibration curve, accuracy, precision, recovery, matrix effect and stability. The results show good linear relationship with …
