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Nutrition-Related Practices And Attitudes Of Kansas Skipped-Generation(S) Caregivers And Their Grandchildren, Mary Meck Higgins, Bethany J. Murray 2010 Kansas State University

Nutrition-Related Practices And Attitudes Of Kansas Skipped-Generation(S) Caregivers And Their Grandchildren, Mary Meck Higgins, Bethany J. Murray

Nutrition and Health Sciences -- Faculty Publications

Despite growing numbers, the nutrition practices and attitudes of skipped-generation(s) kinship caregivers regarding feeding the dependent children in their care have not been examined. In this qualitative study, transcriptions of semi-structured interviews with 19 female and four male skipped-generation(s) Kansas caregivers (ages 47 to 80, 92% non-Hispanic whites, 83% female, 78% grandparents and 22% great-aunt or great-grandparent caregivers; caring for a range of one to four children, ages three to 18, for an average of nine years) were content analyzed for how their nutrition-related practices and attitudes had changed since parenting the first time. Sub-themes regarding practices included ...


Liver Injury In Acute Fatty Liver Of Pregnancy: Possible Link To Placental Mitochondrial Dysfunction And Oxidative Stress, Sathish Kumar Natarajan, Kavitha R. Thangaraj, C. E. Eapen, Anup Ramachandran, Ashis Mukhopadhya, Mathews Mathai, Lakshmi Seshadri, Abraham Peedikayil, Banumathi Ramakrishna, Kunnissery A. Balasubramanian 2010 Christian Medical College

Liver Injury In Acute Fatty Liver Of Pregnancy: Possible Link To Placental Mitochondrial Dysfunction And Oxidative Stress, Sathish Kumar Natarajan, Kavitha R. Thangaraj, C. E. Eapen, Anup Ramachandran, Ashis Mukhopadhya, Mathews Mathai, Lakshmi Seshadri, Abraham Peedikayil, Banumathi Ramakrishna, Kunnissery A. Balasubramanian

Nutrition and Health Sciences -- Faculty Publications

Acute fatty liver of pregnancy (AFLP) is a rare disorder which is fatal if not recognized and treated early. Delivery of the feto-placental unit results in dramatic improvement in maternal liver function, suggesting a role for the placenta. However, the mechanisms by which defects in the fetus or placenta lead to maternal liver damage are not well understood and form the focus of this study. Placenta and serum were obtained at delivery from patients with AFLP, and placental mitochondria and peroxisomes were isolated. Placental mitochondrial function, oxidative stress, and fatty acid composition as well asserumantioxidants, oxidativeandnitrosative stress markers,andfatty acid ...


Predicting Successful Aging In A Population-Based Sample Of Georgia Centenarians, Jonathan Arnold, Jianliang Dai, Lusine Nahapetyan, Ankit Arte, Mary Ann Johnson, Dorothy Hausman, Willard L. Rodgers, Robert Hensley, Peter Martin, Maurice MacDonald, Adam Davey, Ilene C. Siegler, S. Michal Jazwinski, Leonard W. Poon 2010 University of Georgia

Predicting Successful Aging In A Population-Based Sample Of Georgia Centenarians, Jonathan Arnold, Jianliang Dai, Lusine Nahapetyan, Ankit Arte, Mary Ann Johnson, Dorothy Hausman, Willard L. Rodgers, Robert Hensley, Peter Martin, Maurice Macdonald, Adam Davey, Ilene C. Siegler, S. Michal Jazwinski, Leonard W. Poon

Nutrition and Health Sciences -- Faculty Publications

Used a population-based sample (Georgia Centenarian Study, GCS), to determine proportions of centenarians reaching 100 years as (1) survivors (43%) of chronic diseases first experienced between 0–80 years of age, (2) delayers (36%) with chronic diseases first experienced between 80–98 years of age, or (3) escapers (17%) with chronic diseases only at 98 years of age or older. Diseases fall into two morbidity profiles of 11 chronic diseases; one including cardiovascular disease, cancer, anemia, and osteoporosis, and another including dementia. Centenarians at risk for cancer in their lifetime tended to be escapers (73%), while those at risk for ...


Genistein Mediates Perturbations In One-Carbon Metabolism During Diet-Induced Folate Deficiency, Eric Nonnecke 2010 Iowa State University

Genistein Mediates Perturbations In One-Carbon Metabolism During Diet-Induced Folate Deficiency, Eric Nonnecke

Graduate Theses and Dissertations

Homeostasic balance of one-carbon metabolism is highly dependent upon derivatives of folate and is vital for the prevention of multiple disease states, including vascular disease, birth defects, neurological disorders, and cancer. Homocysteine, a non-protein forming amino acid, accumulates in the absence of 5-methyltetrahydrofolate and represents both a marker for reduced cellular methylation potential and elicitor of pathogenesis (Cattaneo, 1999). In addition to cofactors like folate, vitamin B6, B12, and B2, various bioactive compounds are believed to influence the same cellular processes either directly or indirectly (Strain et al., 2004). The isoflavone genistein exhibits estrogen-like properties and has been implicated in ...


Utility Of The Sensewear Pro 3 Armband Monitor And The Weight Management System For Evaluating Energy Balance In Adults, Annette Sarah Mcguire 2010 Iowa State University

Utility Of The Sensewear Pro 3 Armband Monitor And The Weight Management System For Evaluating Energy Balance In Adults, Annette Sarah Mcguire

Graduate Theses and Dissertations

The progression of the obesity epidemic stems directly from the inability of individuals to achieve a balance between energy expenditure (EE) and energy intake (EI). To enhance research on obesity prevention it is important to develop and validate instruments that can facilitate monitoring of energy balance (EB) under free-living conditions. The SenseWear Pro 3 armband (SWA) monitor and associated weight management system (WMS) offer promise as a non-invasive method suitable for energy balance research. The primary purpose of the study was to evaluate the utility of the SWA and WMS to assess EB during a week of free-living activity. A ...


Exercise Prevents Hyperhomocysteinemia In A Folate-Deficient Mouse Model, Joshua Charles Neuman 2010 Iowa State University

Exercise Prevents Hyperhomocysteinemia In A Folate-Deficient Mouse Model, Joshua Charles Neuman

Graduate Theses and Dissertations

Normal folate, homocysteine, and methyl group metabolism is critical for maintaining optimum health, and perturbations in these mechanisms may lead to a number of pathological conditions such as diabetes, cardiovascular disease, neural tube defects, and cancer. Hyperhomocysteinemia is a condition that occurs when blood plasma concentrations of homocysteine exceed 15μM and has been implicated as an independent risk factor in cardiovascular disease. Specifically, populations with genetic polymorphisms in folate-dependent remethylation or poor B-vitamin status are at risk for this condition. This study was conducted in order to determine the influence of exercise on homocysteine and methyl group metabolism in a ...


Promoting Health Literacy Through The School Nutrition Environment, Amber Ann Appleton 2010 Iowa State University

Promoting Health Literacy Through The School Nutrition Environment, Amber Ann Appleton

Graduate Theses and Dissertations

Background: Health literacy and food perceptions influence health knowledge, behaviors, and subsequent health status. Improving health literacy and modifying food perceptions through social marketing nutrition messaging may prove beneficial, particularly in youth. Presently, schools are sending youth mixed messages. Healthful eating behaviors are taught and promoted in the classroom, but not modeled in the school nutrition environment; items sold in competitive food venues (i.e. vending, ala carte, school stores) are typically energy dense, nutrient poor. Thus, the purpose of this study was to improve student health literacy and food perceptions through social marketing nutrition messaging and improving the quality ...


Beta-Glucan Effects On Pasting Properties And Potential Health Benefits Of Flours From Different Oat Lines, Yanjun Liu 2010 Iowa State University

Beta-Glucan Effects On Pasting Properties And Potential Health Benefits Of Flours From Different Oat Lines, Yanjun Liu

Graduate Theses and Dissertations

Oats (Avena sativa), a multifunctional crop, is nutritionally superior to many other unfortified cereals. Whole oat grains are good source of dietary fiber, especially soluble beta-glucans, protein, vitamins, minerals, and antioxidants.

Numerous laboratory and clinical studies have demonstrated that consumption of oat-based products can lower serum cholesterol levels, reduce glucose uptake, decrease plasma insulin response, and control weight through prolonged satiety (Davidson and others 1991; Braaten and others 1994; Mylkki and Virtanen 2001; Wood 2007). These physiological effects of oats are primarily attributable to the elevation of viscosity in the gastrointestinal tract (Wood 2007), caused mainly by the beta-glucans. The ...


Estimating Intake Of Omega-3 Fatty Acids During Pregnancy, Rebecca Me Filipowicz 2010 Iowa State University

Estimating Intake Of Omega-3 Fatty Acids During Pregnancy, Rebecca Me Filipowicz

Graduate Theses and Dissertations

Estimating intake of omega-3 fatty acids during pregnancy

by

Rebecca Mary Elizabeth Filipowicz

The essential omega 3 (n-3) fatty acid alpha-linoleic acid (ALA) and the n-3 fatty acids eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA) are important structural components of cell membranes. During pregnancy a steady supply of n-3 fatty acids is required to support positive fetal development (1-4) and maternal health (5-9). Over the past decade, extensive clinical research has focused on the role of DHA during pregnancy because it is incorporated into fetal brain grey matter and retina cell membranes more than any other n-3 ...


Resistant-Starch Formation In High-Amylose Maize Starch, Hongxin Jiang 2010 Iowa State University

Resistant-Starch Formation In High-Amylose Maize Starch, Hongxin Jiang

Graduate Theses and Dissertations

A new public high-amylose maize line, GEMS-0067, has been developed by USDA-ARS Germplasm Enhancement of Maize (GEM) Project. GEMS-0067 maize line is a homozygous mutant of amylose-extender (ae) gene and high-amylose modifier (HAM) gene(s). GEMS-0067 starches consist of 83.1-85.6% apparent amylose and 39.4%-43.2% resistant starch (RS), which are larger than the ae single-mutant starches (61.7-67.7% and 11.5%-19.1%, respectively) and normal maize starch (~30% and <1%, respectively). Maize ae-mutant starches consisted of spherical and elongated granules (up to 32%), which differed from normal maize starch, consisting of spherical and angular granules. The objectives of this study were to understand the mechanism of the RS formation in the maize ae-mutant starches, the crystalline structures of the maize ae-mutant starches, the formation of RS and elongated starch granules during the kernel development, and how dosage of the HAM gene(s) affected the structures and properties of endosperm starch of maize ae-mutant. We analyzed the structures and properties of the RS residues that remained after enzymatic hydrolysis of the maize ae-mutant starches at 95-100C (AOAC method 991.43). The RS residues consisted of mainly partially hydrolyzed amylose and intermediate component (IC) and were more concentrated in the elongated starch granules and the outer layer of the spherical granules. The RS residues displayed the B-type polymorph and had gelatinization temperatures above 100C. These results suggested that long-chain double-helical crystallites of amylose/IC were present in the native maize ae-mutant starches. The amylose/IC crystallites maintained the semi-crystalline structures at 95-100C and were resistant to enzymatic hydrolysis at 95-100C.

To understand the crystalline structures of the maize ae-mutant starches, we prepared Naegeli dextrins of the starches using sulfuric acid (15.3%, v/v) hydrolysis ...


Reduction In Mucosal Barrier Markers With Soy Protein Diet But Not Lactobacillus Rhamnosus Gg In Dss-Treated Mice, Huanyi Jiang 2010 Iowa State University

Reduction In Mucosal Barrier Markers With Soy Protein Diet But Not Lactobacillus Rhamnosus Gg In Dss-Treated Mice, Huanyi Jiang

Graduate Theses and Dissertations

The incidence of inflammatory bowel diseases (IBD) increased recently. Within the colon, the family of mucins and trefoil factors (TFF) facilitate mucosal protection. Our objective was to determine if diet and/or administration of LGG would attenuate DSS-induced colitis by altering expression of the MUC and TFF genes. C57BL/6 mice were fed diets containing casein, soy, or whey protein with or without LGG for 12 days. Seven days after LGG, the mice were given 2% DSS for 4 days. One day after DSS treatment the colonic and cecal tissues and fecal samples were collected and analyzed by quantitative RT-PCR ...


Deactivation Of Soybean Agglutinin By Enzyme Hydrolysis And Identification Of Active Peptides From Soy Proteins, Yating Ma 2010 Iowa State University

Deactivation Of Soybean Agglutinin By Enzyme Hydrolysis And Identification Of Active Peptides From Soy Proteins, Yating Ma

Graduate Theses and Dissertations

A series of experiments was conducted to eliminate activity of SBA. Deglycosylation decreased activity of SBA by 21%, but not as much as denaturation (23-53%). Single enzymes did not hydrolyze native SBA, but hydrolyzed heat- or organic solutes-denatured SBA. Even after hydrolysis, activity of SBA still was not fully eliminated (44-62% residual). A combination of multiple enzymes with thermolysin fully deactivated heat- or organic solutes-treated SBA. Tea polyphenols, metal ions, chelating agents were also tested and had no significant effect on deactivating SBA. N-acetyl-galactosamine (GalNAc)-agrose beads specifically removed SBA from a protein mixture, but not fully and activity of ...


31p Nmr Phospholipid Profiling Of Soybean Emulsion Recovered From Aqueous Extraction, Linxing Yao, Stéphanie Jung 2010 Iowa State University

31p Nmr Phospholipid Profiling Of Soybean Emulsion Recovered From Aqueous Extraction, Linxing Yao, Stéphanie Jung

Food Science and Human Nutrition Publications

The quantity and composition of phospholipids in full-fat soybean flour, flakes, and extruded flakes and in the cream fraction recovered after aqueous extraction (AEP) and enzyme-assisted aqueous extraction (EAEP) of these substrates were studied with 31P NMR. Extruded flakes had significantly more phosphatidic acid (PA) than flakes and flour prior to aqueous extraction. The PA content of the cream recovered after AEP and EAEP of extruded flakes was similar to that of the starting material, whereas the PA content of the creams from flour and flakes significantly increased. Changes in the PA content could be explained by the action ...


Enrichment Of Echinacea Angustifolia With Bauer Alkylamide 11 And Bauer Ketone 23 Increased Anti-Inflammatory Potential Through Interference With Cox-2 Enzyme Activity, Carlie A. LaLone, Nan Huang, Ludmila Rizshsky, Man-Yu Yum, Navrozedeep Singh, Cathy Hauck, Basil J. Nikolau, Eve S. Wurtele, Marian L. Kohut, Patricia A. Murphy, Diane F. Birt 2010 Iowa State University

Enrichment Of Echinacea Angustifolia With Bauer Alkylamide 11 And Bauer Ketone 23 Increased Anti-Inflammatory Potential Through Interference With Cox-2 Enzyme Activity, Carlie A. Lalone, Nan Huang, Ludmila Rizshsky, Man-Yu Yum, Navrozedeep Singh, Cathy Hauck, Basil J. Nikolau, Eve S. Wurtele, Marian L. Kohut, Patricia A. Murphy, Diane F. Birt

Food Science and Human Nutrition Publications

Bauer alkylamide 11 and Bauer ketone 23 were previously found to be partially responsible forEchinacea angustifolia anti-inflammatory properties. This study further tested their importance using the inhibition of prostaglandin E2 (PGE2) and nitric oxide (NO) production by RAW264.7 mouse macrophages in the absence and presence of lipopolysaccharide (LPS) and E. angustifolia extracts, phytochemical enriched fractions, or pure synthesized standards. Molecular targets were probed using microarray, qRT-PCR, Western blot, and enzyme assays. Fractions with these phytochemicals were more potent inhibitors of LPS-induced PGE2 production than E. angustifolia extracts. Microarray did not detect changes in transcripts with ...


Multidisciplinary Survivorship Care, Sue N. Gardner RN, MSN, CRNP, AOCNP, Gregory Harper MD, PhD, Dorothy Morrone RNC, MS, OCN, Jennifer Brennan RD, CSO, Andrea Geshan RN, MSW, Diane Brong LCSW, Jennifer Roeder MSPT, MBA 2010 Lehigh Valley Health Network

Multidisciplinary Survivorship Care, Sue N. Gardner Rn, Msn, Crnp, Aocnp, Gregory Harper Md, Phd, Dorothy Morrone Rnc, Ms, Ocn, Jennifer Brennan Rd, Cso, Andrea Geshan Rn, Msw, Diane Brong Lcsw, Jennifer Roeder Mspt, Mba

Department of Medicine

No abstract provided.


Beliefs And Attitudes Toward Vegetarian Lifestyle Across Generations, Peter Pribis, Rose C. Pencak, Tevni Grajales 2010 Andrews University

Beliefs And Attitudes Toward Vegetarian Lifestyle Across Generations, Peter Pribis, Rose C. Pencak, Tevni Grajales

Faculty Publications

The objective of the study was to examine whether reasons to adopt vegetarian lifestyle differ significantly among generations. Using a Food Frequency Questionnaire (FFQ), we identified that 4% of the participants were vegans, 25% lacto-ovo-vegetarians, 4% pesco-vegetarians and 67% non-vegetarian. Younger people significantly agreed more with the moral reason and with the environmental reason. People ages 41-60 significantly agreed more with the health reason. There are significant differences across generations as to why people choose to live a vegetarian lifestyle. © 2010 by the authors; licensee MDPI, Basel, Switzerland.


Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek 2009 The University of Tennessee

Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek

Seung J Baek

Expression of cyclooxygenases (COX) and lipoxygenases (LOX) has been linked to many pathophysiological phenotypes, including cell adhesion. However, many current approaches to measure cellular changes are performed only in a fixed-time point. Since cells dynamically move in conjunction with the cell matrix, there is a pressing need for dynamic or time-dependent methods for the investigation of cell properties. In the presented study, we used stable human colorectal cancer cell lines ectopically expressing COX-1, COX-2, and 15LOX-1, to investigate whether expression of COX-1, COX-2, or 15LOX-1 would affect cell adhesion using our opto-electric methodology. In a fixed-time point experiment, only COX-1- ...


Nsaid-Activated Gene-1 As A Molecular Target For Capsaicin-Induced Apoptosis Through A Novel Molecular Mechanism Involving Gsk3beta, C/Ebpbeta And Atf3, Seung Baek, Seong-Ho Lee, Chutwadee Krisanapun 2009 The University of Tennessee

Nsaid-Activated Gene-1 As A Molecular Target For Capsaicin-Induced Apoptosis Through A Novel Molecular Mechanism Involving Gsk3beta, C/Ebpbeta And Atf3, Seung Baek, Seong-Ho Lee, Chutwadee Krisanapun

Seung J Baek

Capsaicin, a natural product of the Capsicum species of red peppers, is known to induce apoptosis and suppress growth. Non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) is a cytokine associated with pro-apoptotic and antitumorigenic property in colorectal and lung cancer. Our data demonstrate that capsaicin leads to induction of apoptosis and up-regulates NAG-1 gene expression at the transcriptional level. Overexpression of CCAAT/enhancer binding protein beta (C/EBPbeta) caused a significant increase of basal and capsaicin-induced NAG-1 promoter activity. We subsequently identified C/EBPbeta binding sites in the NAG-1 promoter responsible for capsaicin-induced NAG-1 transactivation. Electrophoretic mobility shift assay and chromatin immunoprecipitation ...


A Potential Proliferative Gene, Nudt6, Is Down-Regulated By Green Tea Catechins At The Posttranscriptional Level, Seung Baek, Mugdha Sukthankar, CK Choi, A English, JS Kim 2009 The University of Tennessee

A Potential Proliferative Gene, Nudt6, Is Down-Regulated By Green Tea Catechins At The Posttranscriptional Level, Seung Baek, Mugdha Sukthankar, Ck Choi, A English, Js Kim

Seung J Baek

The main aims of this study were to elucidate the effect of green tea catechins on Nudix-type motif 6 (NUDT6) suppression and to characterize NUDT6's biological activity. Our microarray data showed that the green tea component epicatechin-3-gallate suppressed NUDT6 expression, and this was confirmed by RT-PCR. Subsequently, the use of different catechins showed that the effect of epigallocatechin-3-gallate (EGCG) was stronger than that of other catechins. At the posttranscriptional level, EGCG decreased the RNA stability of NUDT6, indicating it as a potential mechanism of NUDT6 suppression. Further cloning of the 3' untranslated region of human NUDT6 mRNA resulted in ...


Screen-Related Sedentary Behaviors: Children's And Parents' Attitudes, Motivations, And Practices, Meizi He, Leonard Piche, Charlene Beynon, Stewart Harris 2009 The University of Western Ontario - Brescia University College

Screen-Related Sedentary Behaviors: Children's And Parents' Attitudes, Motivations, And Practices, Meizi He, Leonard Piche, Charlene Beynon, Stewart Harris

Leonard Piché

OBJECTIVE: To investigate school-aged children's and parents' attitudes, social influences, and intentions toward excessive screen-related sedentary behavior (S-RSB).

DESIGN: A cross-sectional study using a survey methodology.

SETTING: Elementary schools in London, Ontario, Canada.

PARTICIPANTS: All grades 5 and 6 students, their parents, and their teachers in the participating schools were invited to voluntarily participate; 508 student-parent pairs completed the surveys.

MAIN OUTCOME MEASURE: Children's screen-related behaviors.

ANALYSIS: Data were analyzed using the Independent Student t test to compare differences of continuous variables and the chi-square test to test for differences of categorical variables.

RESULTS: Children spent 3.3 ...


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