Neuroscience and Neurobiology Commons^™

Ciliary Arl14b In Vision, Alexis Breann Crockett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Primary cilia are highly specialized organelles that regulate signaling, trafficking, and sensory perception across multiple tissues, including the retina. The small GTPase ADP Ribosylation Factor-Like Protein 13B (ARL13B) is an atypical ARF-family protein enriched in cilia. ARL13B acts as a guanine exchange factor (GEF) for another small GTPase ARF-like Protein 3 (ARL3). Mutations in ARL13B cause Joubert syndrome, a ciliopathy characterized by brain malformation, developmental delays, and retinal degeneration. Despite ARL13B’s known ciliary localization and clinical relevance, ARL13B’s precise functions in cilia remain poorly defined. This dissertation investigates the cilia-specific roles and GEF-specific roles of ARL13B in photoreceptors using two …

Sleep, Endocannabinoid Signaling, And Cortical Processing In Mouse Models Of Autism Spectrum Disorder, Bryan Rodriguez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sleep plays a crucial role in memory consolidation, neuronal plasticity, and cognitive function. Individuals with autism spectrum disorder (ASD) often experience sleep disturbances, which are positively correlated with symptom severity. However, on the surface, sleep architecture is remarkably normal under baseline conditions in many rodent models of ASD. The goals if this dissertation were to investigate whether regulation of sleep, biochemical signaling during sleep, and downstream consequences of sleep on behavior are altered in two mouse models of ASD. In Aim 1, we tested the hypothesis that sleep phenotypes emerge in ASD models under conditions of high sleep pressure. We …

Hippocampal Insulin Exists! In Vivo Sampling Methods, Basal Concentrations, Recurrent Hypoglycemia, And Behavioral Extracellular Insulin Responses, Starlette R. Douglass

Electronic Theses & Dissertations (2024 - present)

Insulin has widespread actions within the brain, including energy homeostasis, modulation of feeding, and as a key component of memory processes. Further, brain insulin dysfunction correlates with Alzheimer's disease and other neurodegenerative disorders. To this point, measurement of hippocampal insulin has been limited. Determining in vivo hippocampal insulin levels both at a healthy baseline and after, e.g., induction of a disease state or in response to cognitive challenge, is essential to advance our understanding of both insulin's procognitive actions and to guide potential therapeutic interventions.

We first sought to establish a sampling technique for insulin within awake and moving rats …

The Multifaceted Role Of Glutamate At Excitatory And Inhibitory Synapses, Maurice A. Petroccione

Electronic Theses & Dissertations (2024 - present)

Our thoughts, memories, and actions are finely controlled by the coordinated activity of neural circuits in the brain. Prior work has identified a multitude of molecular mechanisms that contribute to the generation of these higher order functions, yet we know very little about how our ability to learn and perform habitual actions change depending on how far neurotransmitters released from neurons can travel away from their point of release. This is the main focus of my work, which I carried out using a diverse toolbox and focusing on two brain regions: the hippocampus and the striatum. The hippocampus controls learning …

Technological Development Of In-Cell Nmr: Microfluidic, Metabolomic, And Fluxomic Approaches, Nicholas Sciolino

Electronic Theses & Dissertations (2024 - present)

In the fields of structural biology, metabolomics, and fluxomics, there is a driving, technique-based concern when it comes to high-resolution spectrometry and its marriage to biological and physiological relevance. Sample preparation often involves cellular perturbation, lysate separation schemes, combinations with non-biological reagents, and timeline slack. Conceptually, the farther we get from the cell, the greater the potential disparity between what exists in vivo and our in vitro manipulations. This is particularly true when seeking to collect data on transfected targets of interest, and on sensitive metabolic equilibria that occur on the order of minutes. The question remains: if we are …

Integrated Multi-Omics Analyses Of Synaptosomes Revealed Synapse-Associated Novel Targets In Alzheimer’S Disease, Subodh Kumar, Enrique Ramos, Axel Hidalgo, Daniela Rodarte, Bhupender Sharma, Melissa M. Torres, Davin Devara, Shrikanth Gadad

School of Medicine Publications

Synapse dysfunction is an early event in Alzheimer’s disease (AD) caused by various factors, including amyloid beta, p-tau, inflammation, and aging. However, the precise molecular mechanism underlying synapse dysfunction in AD remains largely unknown. To understand this, we comprehensively analyzed the synaptosomes fraction in post-mortem brain samples from AD patients and cognitively normal individuals. We conducted high-throughput transcriptomic analyses to identify changes in microRNA (miRNA) and mRNA levels in synaptosomes extracted from the brains of unaffected individuals and those with AD. Additionally, we performed mass spectrometry analysis of synaptosomal proteins in the same sample group. These analyses revealed significant differences …

Determination Of Electrochemical Parameters For Predicting Reaction Mechanism And Algorithmic Approaches To Pain Assessment, Huize Xue

Dissertations

This dissertation introduces novel advancements in electrochemical kinetics and pain assessment, structured into two main parts. The first part focuses on the comprehensive analysis of the kinetic and mechanistic aspects of electrochemical reactions, utilizing a combination of experimental techniques and simulation methods. A new software tool, Envismetrics, was developed using Python to facilitate the analysis of complex electrochemical data, including cyclic voltammetry (CV), chronoamperometry (CA), and hydrodynamic voltammetry (HDV). The software was rigorously tested and validated with well-characterized redox systems such as the ferricyanide/ferrocyanide couple, dimethylamine borane (DMAB), and Per- and Polyfluoroalkyl Substances (PFAS). It was successfully used to determine …

Can Animal Consciousness Be Resolved Scientifically?, Yoram Gutfreund

Animal Sentience

Carolyn Ristau's biography of Donald Griffin is a comprehensive work that sheds light on a seminal chapter in the history of ethology—the ambitious attempt to establish a research field dedicated to animal consciousness. Although Donald Griffin's endeavor ultimately fell short of its revolutionary goals, it catalyzed significant progress in understanding evolution and mechanisms of animal cognition.

Regulation And Function Of Mec-2 Alternative Splicing In Single Cells, Canyon Calovich-Benne

Biological Sciences Theses and Dissertations

One mechanism for generating diversity at the single cell level is selective expression of a unique set of transcription factors (TFs) and RNA binding proteins (RBPs). These regulatory factors contribute to functions of cells by controlling cell fate, turning genes on and off, and processing of transcripts either co- or post-transcriptionally. Many genes, especially neuronal genes, have multiple different splicing events, promoters, and/or polyadenylation sites. Post-transcriptional coordination is in the infancy of being studied and the functional consequences of this coupling at the single cell level has yet to be explored. Here we show, mec-2 coordination of alternative transcription start …

Novel Digital Anomalies, Hippocampal Atrophy, And Mutations Expand The Genotypic And Phenotypic Spectra Of Cnksr2 In The Houge Type Of X-Linked Syndromic Intellectual Development Disorder (Mrxshg), Mohammad Reza Ghasemi, Sahand Tehrani Fateh, Afif Ben-Mahmoud, Vijay Gupta, Lara G. Stühn, Gaetan Lesca, Nicolas Chatron, Konrad Platzer, Patrick Edery, Hossein Sadeghi, Bertrand Isidor, Benjamin Cogné, Heidi L. Schulz, Ilona Krauspe-Stübecke, Radhakrishnan Periyasamy, Sheela Nampoothiri, Reza Mirfakhraie, Sahar Alijanpour, Steffen Syrbe, Ulrich Pfeifer, Stephanie Spranger, Kathrin Grundmann-Hauser, Tobias B. Haack, Maria T. Papadopoulou, Tayrine Da Silva Gonçalves, Eleni Panagiotakaki, Alexis Arzimanoglou, Seyed Hassan Tonekaboni, Yves Lacassie, Et Al

School of Medicine Faculty Publications

The Houge type of X-linked syndromic intellectual developmental disorder (MRXSHG) encompasses a spectrum of neurodevelopmental disorders characterized by intellectual disability (ID), language/speech delay, attention issues, and epilepsy. These conditions arise from hemizygous or heterozygous deletions, along with point mutations, affecting CNKSR2, a gene located at Xp22.12. CNKSR2, also known as CNK2 or MAGUIN, functions as a synaptic scaffolding molecule within the neuronal postsynaptic density (PSD) of the central nervous system. It acts as a link connecting postsynaptic structural proteins, such as PSD95 and S-SCAM, by employing multiple functional domains crucial for synaptic signaling and protein–protein interactions. Predominantly expressed in dendrites, …

How Do Anxiety, Stress, Impulsiveness, And Sleep Quality Affect Memory, Hodavia Tshiya Kaseya

Online Theses and Dissertations

Memory is a critical cognitive function that enables individuals to retain, recall, and apply knowledge across various contexts. Different memory systems, including working memory and long-term memory, collaborate to process and store information. Factors such as stress, sleep disturbances, anxiety, and impulsiveness can impact memory encoding and retrieval, especially in college students, whose academic success depends heavily on memory performance. This study examined the relationship between these everyday factors and memory performance by analyzing brain activity during memory encoding using Electroencephalography (EEG) and the Difference due to memory (DM) event-related potential (ERP) effect, to understand how stress, sleep, anxiety, and …

Investigating Tumor Growth And Regulation In Supratentorial Ependymomas; The Impact Of Dlk1 And Egr1 Knockout, Om Sinojia

Honors Scholar Theses

Ependymomas (EPNs) are primary brain tumors that often arise from radial glial cells lining the ventricular system. Supratentorial ependymomas (ST-EPNs) are particularly aggressive, and understanding the molecular factors driving their growth is critical for developing targeted therapies. This study investigates the roles of DLK1 and EGR1, key regulators in cellular differentiation and tumorigenesis, in the development of ST-EPNs. We utilized genetically engineered mouse models to induce postnatal knockouts of DLK1 and EGR1 and evaluated tumor growth using histological and imaging techniques. Tumor area was quantified across multiple brain sections from both male and female mice. DLK1 knockout brains exhibited consistent …

Conceptual Curiosity: Sense(Un)Making, Rebekah L. Cowell

Proceedings from the Document Academy

Across disciplines, sensemaking is a theoretical and methodological research program, over forty years in development, that has been treated as a framework for understanding how humans behaviorally respond to external information sources and interpret to construct and actualize actionable outcomes; however, sensemaking is an interlinking process that does not operate in singularity–behaviorally or actionably–it is neurobiological (autonomous drive), cognitive (perceptual and predictive), and psychological (congruency in meaning).

This paper enlarges the conceptual framework for studying sensemaking and redefines it by addressing what sensemaking is, does, and needs. At the core of this conversation is addressing the absence of knowledge and …

Are There Neurological Impacts Of Exposure To A Burned Wildfire Site As It Recovers?, Rubye Strickland

Undergraduate Honors Theses

Wildfires are increasing with climate change, making the human health implications of exposure to burned soils an essential area of research. Metals associated with fire-impacted soils can be inhaled or accidentally ingested by surrounding communities, causing health risks. Several studies have evaluated the health impact of wildfires, but few have done so from a neurological perspective. This study explores metal changes in burned soils over time as a site naturally recovers from wildfire and how these changes may impact human neurological health. Soil samples were collected from the burned area 0, 3, 6, 9, and 12 months after the fire. …

Sk609, A Novel Dopamine D3 Receptor Agonist And Norepinephrine Transporter Blocker With Putative Pro-Cognitive Actions, Does Not Induce Psychostimulant-Like Increases In Risky Choice During Probabilistic Discounting, Christopher P. Knapp, Brooke Fallon, Sandhya Kortagere, Barry D. Waterhouse, Stan B Floresco, Rachel L Navarra

Rowan-Virtua School of Osteopathic Medicine Departmental Research

RATIONALE: Psychostimulants, such as amphetamine (AMPH) and methylphenidate (MPH), non-selectively elevate extracellular concentrations of the catecholamine neurotransmitters, dopamine (DA) and norepinephrine (NE), and are common pharmacological strategies used to improve prefrontal cortex (PFC)-dependent cognitive dysfunction. However, this approach can be problematic given AMPH has been shown to increase preference for risky choices in a rodent assay of risk/reward decision making. SK609 is a novel NE reuptake blocker that selectively activates DA D3 receptors without affinity for the DA transporter. SK609 has been shown to improve cognitive performance without increasing psychostimulant-like spontaneous locomotor activity, suggesting SK609 may benefit neurocognitive function without …

Sympathetic Innervation Of Interscapular Brown Adipose Tissue Is Not A Predominant Mediator Of Ot-Elicited Reductions Of Body Weight Gain And Adiposity In Male Diet-Induced Obese Rats, Melise Edwards, Ha Nguyen, Andrew Dodson, Adam Herbertson, Mackenzie Honeycutt, Jared Slattery, June Rambousek, Edison Tsui, Tami Wolden-Hansen, Tomasz Wietecha, James Graham, Geronimo Tapia, Carl Sikkema, Kevin O'Brien, Thomas Mundinger, Elaine Peskind, Vitaly Ryu, Peter Havel, Arshad Khan, Gerald Taborsky, James Blevins

Selected Works Temporary Series

Recent studies indicate that central administration of oxytocin (OT) reduces body weight (BW) in high fat diet-induced obese (DIO) rodents by reducing energy intake and increasing energy expenditure (EE). Previous studies in our lab have shown that administration of OT into the fourth ventricle (4V; hindbrain) elicits weight loss and stimulates interscapular brown adipose tissue temperature (TIBAT) in DIO rats. We hypothesized that OT-elicited stimulation of sympathetic nervous system (SNS) activation of IBAT contributes to its ability to activate BAT and reduce BW in DIO rats. To test this, we determined the effect of disrupting SNS activation of IBAT on …

Sympathetic Innervation Of Interscapular Brown Adipose Tissue Is Not A Predominant Mediator Of Ot-Elicited Reductions Of Body Weight Gain And Adiposity In Male Diet-Induced Obese Rats, Edwards, Ha Nguyen, Andrew Dodson, Adam Herbertson, Mackenzie Honeycutt, Jared Slattery, June Rambousek, Edison Tsui, Tami Wolden-Hansen, Tomasz Wietecha, James Graham, Geronimo Tapia, Carl Sikkema, Kevin O'Brien, Thomas Mundinger, Elaine Peskind, Vitaly Ryu, Peter Havel, Arshad Khan, Gerald Taborsky, James Blevins

Departmental Papers (Biology)

Recent studies indicate that central administration of oxytocin (OT) reduces body weight (BW) in high fat diet-induced obese (DIO) rodents by reducing energy intake and increasing energy expenditure (EE). Previous studies in our lab have shown that  administration of OT into the fourth ventricle (4V; hindbrain) elicits weight loss and stimulates interscapular brown adipose tissue temperature (TIBAT) in DIO rats. We hypothesized that OT-elicited stimulation of sympathetic nervous system (SNS) activation of IBAT contributes to its ability to activate BAT and reduce BW in DIO rats. To test this, we determined the effect of disrupting SNS activation of IBAT on …

Neuroplasticity And Creativity: Transformative Potential Of Fibre Arts For Growth And Well-Being, Piper Hutson, James Hutson

Faculty Scholarship

This article explores the transformative potential of fibre arts—such as quilting, weaving, and embroidery—as tools for personal growth, neuroplasticity, and well-being. Drawing on neuroscience and psychology, it demonstrates how engaging with fibre arts stimulates brain regions associated with problem-solving, sensory integration, and adaptability, fostering neural growth and enhancing cognitive flexibility. Fibre arts, through their inherent challenges and opportunities for play, provide a perfect medium for stepping beyond familiar boundaries, cultivating resilience, and reducing stress. This interplay of creativity and tactile engagement is particularly effective due to the historical undervaluation of fibre arts, which makes them novel and accessible mediums that …

Beyond Pathology: Inclusive Mentorship, Accommodations, And Policy Reforms For Autistic Women In The Medical Profession, Piper Hutson, James Hutson

Faculty Scholarship

This article addresses the unique challenges faced by autistic women in the medical profession, particularly in navigating traditional medical frameworks that often marginalize neurodiverse individuals. Drawing from a targeted literature review, this paper highlights the importance of adapting medical curricula, establishing mentorship programs, and reforming policies to foster inclusivity. Autistic women in medicine encounter barriers such as a lack of mentorship tailored to their needs, inadequate accommodations, and systemic bias rooted in the pathologization of autism. The article synthesizes current research to provide evidence-based recommendations that address these barriers. Specific examples of successful mentorship programs demonstrate the critical role of …

Expanding Sensory Kit Utilization Across Age And Contexts: Bridging Gaps For Inclusive Accessibility, Piper Hutson, James Hutson

Faculty Scholarship

Sensory kits, initially developed for neurodivergent children, have seen growing integration into various educational, cultural, and therapeutic contexts. However, their broader applicability, particularly for adults and diverse populations, remains underexplored. This study examines existing practices in the design and use of sensory kits within academic, museum, and inclusion frameworks, highlighting their role in promoting focus, reducing anxiety, and supporting self-regulation. Using case studies from institutions such as the Smithsonian and the Metropolitan Museum of Art, alongside a review of evidence-based interventions like weighted items and fidget tools, the research underscores the potential of these tools in diverse environments. The methodology …