Microbiology Commons^™

High Level Of Persister Frequency In Clinical Staphylococcal Isolates, Sarita Manandhar, Anjana Singh, Ajit Varma, Shanti Pandey, Neeraj Shrivastava

Faculty Publications

Staphylococcus aureus is a notorious human pathogen that causes often lethal systemic conditions that are mostly medical device associated biofilm infections. Similarly, coagulase negative staphylococci are emerging as leading pathogen for nosocomial infections owing to their ability to form biofilm on implanted medical equipment. Chronic in nature, these infections are difficult to treat. Such recalcitrance of these infections is caused mainly due to the presence of persister cells, which exhibit transient yet extreme tolerance to antibiotics. Despite tremendous clinical significance, there is lack of studies on persister cells formation among clinical bacterial isolates. Considering the importance of factors influencing persister …

Computational Analysis Of Myxococcus Xanthus Gliding Motility With Varying Cellular Growth Rate, Laura Batista, Akeisha Belgrave

Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity

This project focuses on determining the effects of varying growth rates on bacteria motility. Cell growth has been shown to affect peptidoglycan biosynthesis, interacting indirectly with the motility complex that spans across the bacteria. This complex adheres to the external surface via focal adhesion complexes that exert a mechanical force to push the cell forward. Affecting bacteria growth rate, affects peptidoglycan biosynthesis, & should therefore affect M. xanthus motility. (Independent Research)

Annual Faculty Research Symposium 2022, Oakwood University

Proceedings

No abstract provided.

Composting During Winter Versus Summer: A Microbial Analysis, Madison Ochs

Honors Theses

Pre-consumer food waste, hay, and woodchips were composted using two different methods of static composting: forced aeration (FA) and natural aeration (NA). The composting process was performed during the winter and summer for 106 and 124 days, respectively. The composting process for both winter and summer processes did not reach the temperature above the 40°C threshold of the thermophilic stage in composting. The microbial communities present during different timepoints (start, peak, and final) along the composting process were analyzed with DNA sequencing techniques and visualized using QIIME 2, an open-source bioinformatics platform. The microbial communities of the winter and summer …

Pseudomonas Aeruginosa Evades Predation By Myxococcus Xanthus, Sarah Joie Beauvais

Symposium of Student Scholars

Multidrug-resistant Pseudomonas aeruginosa causes an estimated 32,600 hospital-acquired-infections and 2,700 estimated deaths in the US in 2017(CDC). Myxococcus xanthus is a strain of myxobacteria that preys on Pseudomonas sp, through the production of antibiotics and digestive enzymes. In previous experiments, P. aeruginosa evaded predation, through an unknown mechanism. Using confocal microscopy, this research investigates if quorum sensing, a cell density-dependent signaling pathway, could be a factor in motility and evasion of M. xanthus. Liquid suspensions of log-phase PA01 were dyed with two fluorescent dyes: SYTO 9 for marking live cells and Propidium iodide (PI) for marking dead cells. …

Microbial Diversity And Community Structure In Sediments Associated With The Seagrass (Thallassia Testudinum) In Apalachicola Bay, Florida, Rahma Ahmed, Thomas Mcelroy, Troy Mutchler

Symposium of Student Scholars

Seagrass is an angiosperm which provides many ecosystem services in coastal areas, such as providing food, shelter and nurseries for many species, and decreasing the impact of waves on shorelines. A global assessment reported that 29% of known seagrass meadows are in a state of decline due to the effects of human activity. Seagrass is commonly found in shallow marine waters where they form meadows containing a microbiome that plays an important role in providing nutrients for seagrass growth, though little is known about the microorganisms within the seagrass meadow sediments. Our project collected sediments from seagrass meadows and adjacent …

Pengaruh Rizobakteri Indigenous Terhadap Serangan Penyakit Budok, Enzim Peroksidase, Dan Pertumbuhan Setek Nilam Aceh, Halimursyadah, Siti Hafsah, Siti Nurminah Nasution, Zuraida

Jurnal Kultivasi

Rizobakteri adalah kelompok mikroba yang aktif dan agressif mengkolonisasi area rizosfir. Mikroba ini mampu berperan sebagai biofertilizer, biostimulan, dan bioprotektan. Tujuan penelitian ini adalah untuk mengetahui jenis isolat rizobakteri indigenous yang berpotensi sebagai agen biokontrol pada patogen Synchytrium pogostemonis penyebab penyakit budok pada tanaman nilam Aceh (Pogostemon cablin Benth.). Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala dan Kebun Percobaan Sektor Timur Fakultas Pertanian, Darussalam Banda Aceh. Waktu pelaksanaan penelitian dimulai pada bulan Januari sampai dengan Mei 2021. Percobaan ini disusun berdasarkan Rancangan Acak Lengkap dengan 15 perlakuan yang terdiri atas 14 perlakuan …

Analysis And Identification Of Lipolytic Bacterial Species For The Degradation Of Wastewater Lipids, Lindsay C. Smoak

Honors College Theses

The aim of this study was to isolate native bacterial strains from the wastewater treatment facilities of Statesboro, GA to identify their lipolytic activities. Obtained bacterial strains were further assessed via morphological and biochemical methods to determine their enzymatic capabilities. Use of the detergent Tween-20 in growth mediums was the first criteria to assess lipase activity, and these isolates were further investigated to quantitatively measure lipase presence and activity. Lipase protein was precipitated and dialyzed to perform a lipase activity assay, followed by Sodium Dodecyl Sulphate–Polyacrylamide Gel Electrophoresis (SDS-PAGE) to confirm the presence of the lipolytic enzymes. Sanger sequencing was …

Pemilihan Teknik Aplikasi Dan Dosis Pupuk Hayati Pelarut Kalium Untuk Meningkatkan Serapan Kalium Dan Pertumbuhan Tanaman Jagung Pada Tanah Inceptisols, Diyan Herdiyantoro, Tualar Simarmata, Mieke Rochimi Setiawati, Nenny Nurlaeny, Benny Joy, Mahfud Arifin, Jajang Sauman Hamdani, Iin Handayani

Jurnal Kultivasi

Salah satu strategi yang diterapkan pada pupuk hayati untuk menunjukkan efek positif pada tanaman yang diinokulasi adalah pemilihan teknik aplikasi dan dosis yang tepat, baik pada tanah, benih, atau kombinasi keduanya. Tujuan dari percobaan ini adalah mendapatkan teknik aplikasi dan dosis pupuk hayati pelarut K yang memberikan hasil terbaik terhadap penyerapan K dan pertumbuhan tanaman jagung (Zea mays L.) pada Inceptisols Jatinangor. Percobaan dilaksanakan pada November 2018-Januari 2019 di rumah kaca Fakultas Pertanian Universitas Padjadjaran. Percobaan menggunakan rancangan acak kelompok dengan 9 perlakuan dan 3 kali ulangan. Perlakuan terdiri dari tanpa perlakuan (kontrol), aplikasi pada benih 400 g.ha-1 dan 800 …

Viability Test Of Halotolerant Nitrogen-Fixing Rhizobacteria On Different Carrier Composition And Application Dosage Of Nitrogen Biofertilizer To Increase Rice Growth On Saline Ecosystems, Fiqriah Hanum Khumairah, Fachruddin Azwari, Mieke Rochimi Setiawati, Betty Natalie Fitriatin, Tualar Simarmata

Jurnal Kultivasi

The use of saline soils as productive agricultural land poses major challenges. The utilization of nitrogen biofertilizer with halotolerant N-fixing rhizobacteria as the active material at the right dosage can increase soil productivity and support plant growth. The aim of this study was to obtain the composition of the carrier material that can maintain rhizobacteria viability, water content, and pH of nitrogen biofertilizer and to obtain the right dosage to increase the growth of rice plants in saline ecosystems. The research location was at Microbiology Laboratory of CV Bintang Asri Arthauly, Bandung and greenhouse of Jayamukti Village, Banyusari District, Karawang …

Investigating Alternative Induction Of The S. Aureus 80a Prophage, Jody Caretti

Student Scholar Showcase

Widespread antibiotic resistance has quickly become one of the most concerning crises affecting modern medicine, especially in cases of Staphylococcal infections, which have become resistant to all ��-lactam antibiotics. As a result, research into alternative forms of treatment for bacterial infections is a top priority. Bacteriophage therapy is a popular candidate for replacing antibiotics, due to the way the viruses interact with the bacteria. The 80�� prophage integrates its DNA into Staphylococcus aureus strain 10616 via the lysogenic cycle, and replicates with the bacterial cell. When stressed, the bacteria stop replicating and the prophage stimulates the lytic cycle, activating transcription …

Exploring The Functionality Of Putative Bop3 Post-Translational Modifications, Liliya Tkachuk

Honors Scholars Collaborative Projects

All eukaryotic cells require that transcribed mRNAs undergo export form the nucleus to the cytoplasm where they can be translated into proteins. This process requires a host of proteins which are conserved between the unicellular budding yeast, S. cerevisiae, and humans. During this process, Mex67 and other associated proteins facilitate the mRNA to travel across the nuclear pore complex (NPC), doorways embedded in the nuclear envelope. Upon the exit of mRNA, Mex67 is released and recycled back into the nucleus to facilitate the export of more mRNA. This occurs through the action of Dbp5, whose activity is regulated through …

Developing An Electroporation Method For Transforming Streptomyces Nymphaeiformis, Heather Knott, Stephen Baron

Honors Projects

Streptomyces species are notoriously difficult to transform. Streptomyces nymphaeiformis is no different, so a method of electroporation was used to attempt to transform the cells. Multiple growth stages were used in order to alter the degree of development of the cell wall. The procedure did not kill the cells, but the cells were not transformed. Due to the lack of transformation with S. nymphaeiformis, transformation was attempted on two other Streptomyces strains, S. lividans and S. coelicolor. Neither was successfully transformed to thiostrepton (tsr) resistance, nor did they grow on a plate lacking thiostrepton. One possibility for the …

Sars-Cov-2 Reveals That Chimeric Agents Are The Bioweapons Of The Future, Rachel Craig

Senior Honors Theses

Bioweapons programs have existed since their development during the Cold War. These biowarfare programs initially utilized naturally occurring pathogens capable of infecting crops, livestock populations, and human populations. Anthrax is a widely exploited bioagent responsible for attacks ranging from the Germans’ deployment in World War I to the mailing of anthrax through the postal service in attempts on U.S. senators’ lives. With the development of genetic manipulations, the Soviet Union began modifying anthrax to resist detection and treatment. With the continued advancement of science and technology, a new bioagent has entered the scene – the man-made chimeric virus. Chimeric viruses …

Microbial Contamination On Ambulance Surfaces: A Systematic Literature Review, Adam Obenza, Patricia Cruz, Mark Buttner, D. Woodard

Environmental & Global Health Faculty Research

Healthcare-associated infections (HAIs) are infections that patients acquire while receiving medical treatment in a healthcare facility. During ambulatory transport, the patient may be exposed to pathogens transmitted from emergency medical service (EMS) personnel or EMS surfaces. The aim of this study was to determine whether organisms commonly associated with HAIs have been detected on surfaces in the patient-care compartment of ambulances. Five electronic databases – PubMed, Scopus, Web of Science, Embase and Google Scholar were used to search for articles using inclusion and exclusion criteria following the PRISMA checklist. Inclusion criteria consisted of articles published in English, between 2009 and …

Life Line Spring 2022, Otterbein Biology And Earth Science Department

Life Line - The Biology Department Newsletter

In this Issue:

A CURE for the Typical STEM Classroom; Attracting Cardinals; Svitana Provides Training to IHS; The Cardinal Science Scholar Program; Mornings with Manatees; Viewing the Department from a New Perspective; Science Lecture Series Features Dr. Tyrone Hayes; Internship with Beinhower Brothers Drilling Company; New Student Chapter: The Botanical Society of America; STEM Art Show 2021; Got Bones?; The Robert and Ann Harting Endowed Award; Grossology Returns; Class Flocks to National Aviary

Characterization Of A Medium-Dependent Streptomyces Developmental Mutant, Dylan Gray, Jennifer A. Bennett

Undergraduate Distinction Papers

Streptomyces coelicolor is a soil-dwelling, Gram-positive Actinobacteria. Streptomyces species produce thousands of secondary metabolites which include many antibiotics used in medicine today. In Streptomyces development, as colonies grow, secondary metabolites are produced, and ultimately mature spores are dispersed. The production of each of these secondary metabolites in wild-type Streptomyces is determined by a distinct biosynthetic pathway. In order to identify new genes for antibiotic production and other aspects of development, an engineered Tn5-derived transposon was used to mutagenize wild-type cells in vivo and produce single, stable insertions.

Colonies that were visually distinct from the wild type were selected for further …

Recently Evolved Francisella-Like Endosymbiont Outcompetes An Ancient And Evolutionarily Associated Coxiella-Like Endosymbiont In The Lone Star Tick (Amblyomma Americanum) Linked To The Alpha-Gal Syndrome, Deepak Kumar, Surendra Raj Sharma, Abdulsalam Adegoke, Ashley Kennedy, Holly C. Tuten, Andrew Y. Li, Shahid Karim

Faculty Publications

Background: Ticks are hematophagous arthropods that transmit various bacterial, viral, and protozoan pathogens of public health significance. The lone star tick (Amblyomma americanum) is an aggressive human-biting tick that transmits bacterial and viral pathogens, and its bites are suspected of eliciting the alpha-gal syndrome, a newly emerged delayed hypersensitivity following consumption of red meat in the United States. While ongoing studies have attempted to investigate the contribution of different tick-inherent factors to the induction of alpha-gal syndrome, an otherwise understudied aspect is the contribution of the tick microbiome and specifically obligate endosymbionts to the establishment of the alpha-gal …

College Of Natural Sciences Newsletter, March & April 2022, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 3, Issue 3

Page 1 Dean's Message
Page 2-3 Awards and Recognition
Page 4 2022 URSCAD - CNS Snaps
Page 5 Geography Convention Recap
Page 6 Attention Students!
Page 7-8 Media Coverage of CNS
Page 9 Spring Break Outreach, Adopt the Pantry,
Page 10 Discovery on Tap Event
Page 11 CNS Students Visit State Capital
Page 12 Celebrating 50 Years of Sally
Page 13 Open PRAIRIE Data
Page 14 Grants Awarded and Bio-Micro Day of Scholars
Page 15 -17 2022 Geography Convention Snaps
Page 18 Sally Krueger's 50th Work Anniversary

Prevalence Of Bacillus Licheniformis On Northern Saw-Whet Owls (Aegolius Acadicus), Lauren Benedict

Senior Honors Theses

Birds are hosts to a variety of bacteria and fungi including some that are keratinolytic. These feather degrading bacteria, including Bacillus licheniformis, are capable of degrading β-keratin which is a major component of feathers. While there is little evidence to indicate that feather degrading bacteria are capable of degrading feathers on live birds, there is ample evidence to indicate that many bird species experience altered feather coloration. There is significant variation in the reported prevalence of B. licheniformis on birds with percentages ranging from 6.7% to 99%. This study sought to provide further support for an overall prevalence of …