Evaluation Of Physiological Quality Of Seed On Yield Of Yellow And Black Soybean Treated With Biological Seed Coating,
2023
Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran
Evaluation Of Physiological Quality Of Seed On Yield Of Yellow And Black Soybean Treated With Biological Seed Coating, Muhamad Kadapi, Priscilla Olga Salim, Sumadi
Jurnal Kultivasi
The demand for soybeans as one of cereal commodities increases every year, however, to meet the production and demand level is still challenging in Indonesia. One of the causes of low soybean production is stress during cultivation, lack of nutrients, or poor quality of soybean seeds. One solution is to increase seed protection by coating the seeds with an environmentally friendly biological seed coating. This study aims to obtain the best biological seed coating for increasing soybean seed viability, vigor, and yield. The research was conducted at the Faculty of Agriculture Experimental Station of Universitas Padjadjaran, Jatinangor from August to …
Increased Coding Potential Of Bovine Herpesvirus 1,
2023
Mississippi State University
Increased Coding Potential Of Bovine Herpesvirus 1, Victoria Jefferson
Theses and Dissertations
Bovine respiratory disease (BRD) costs the cattle industry millions of dollars in costs in treatment and loss every year in the United States. A significant pathogen often contributes to BRD is Bovine Herpesvirus 1 (BoHV-1), a double stranded DNA virus with the ability to establish latency in the trigeminal ganglia and neurons. Primary infection with BoHV-1 results in immunosuppression that increases the risk of secondary bacterial infection and pneumonia. Because herpesviruses infect their hosts for life and can be reactivated in times of stress, BoHV-1 can present a recurring risk of BRD. The following research aims to expand the knowledge …
Allergic Bronchopulmonary Aspergillosis: Radiological And Microbiological Profile Of Patients Presented In An Outpatient Pulmonary Clinic In A Developing Country,
2023
Aga Khan University
Allergic Bronchopulmonary Aspergillosis: Radiological And Microbiological Profile Of Patients Presented In An Outpatient Pulmonary Clinic In A Developing Country, Nousheen Iqbal, Muhammad Irfan, Mustafa Bin Ali Zubairi, Maaha Ayub, Safia Awan, Kauser Jabeen, Ali Bin Sarwar Zubairi
Section of Pulmonary & Critical Care
There is limited data available about allergic bronchopulmonary aspergillosis (ABPA) in Pakistan. The aim of the study was to describe the radiological and microbiological profile of ABPA patients presenting to the outpatient pulmonary clinic of a tertiary care hospital in Karachi, Pakistan. A retrospective study was conducted on ABPA patients who presented to the pulmonary outpatient clinic at Aga Khan University Hospital, Karachi, Pakistan, from January 2017 to December 2019. Data was collected on microbiology and radiology features on predesigned proforma. A total of 7759 asthmatic patients presented at the outpatient pulmonology clinic during the study period. Of the 245 …
Complete Genome Sequences Of Cluster F1 And Cluster B1 Mycobacterium Smegmatis Phages Karhdo And Basato,
2023
University of Nevada, Las Vegas
Complete Genome Sequences Of Cluster F1 And Cluster B1 Mycobacterium Smegmatis Phages Karhdo And Basato, Jireh Kim, Carlos Herrera, Wai Yan Aung, Gino Pablo Gonzales Boyles, Carmina Chavez, Mona Cibulka, Emma Foley, Jose Guerra, Disha Byadarahalli Mohan Kumar, Willow Levrant, Lewis Lim, Jose Llanes, Zachary Kamuela O'Brian, Art Pagaduan, Justin Aaron Richardson, Khristian Rosales, James Schrecengost, Tommy Shin, Gabriel Strong-Lundquist, Winnie Tat, Fritz Vanderford, Isabelle Vrinceanu, Vicky Wang, Stephanie Yang, Christy Strong, Philippos K. Tsourkas, Kurt Regner
Life Sciences Faculty Research
We present the complete genome sequences of Mycobacterium smegmatis phages Karhdo and Basato, isolated in Clark County, Nevada. The phages were isolated and annotated by students enrolled in undergraduate research courses over two semesters at the University of Nevada, Las Vegas.
Developing Disinfection Strategies For Controlling Human Norovirus, Sars-Cov-2, And Clostridioides Difficile Endospores In Long-Term Care Facilities,
2023
Clemson University
Developing Disinfection Strategies For Controlling Human Norovirus, Sars-Cov-2, And Clostridioides Difficile Endospores In Long-Term Care Facilities, Jinge Huang
All Dissertations
Long-term care facilities (LTCFs) provide an environment favorable for the transmission of three critical human pathogens: human norovirus (HuNoV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and Clostridioides difficile. Given residents in LTCFs are susceptible to infections due to their advanced ages and compromised immune systems, effective environmental surface disinfection plays a crucial role in controlling the spread of human pathogens within these settings and, therefore, mitigates the risk of infections caused by these pathogens. This dissertation aimed to assess the efficacy of various types of disinfectants against two HuNoV surrogates [feline calicivirus (FCV) and Tulane virus (TuV)], two …
Ecohiv-Infected Mice Show No Signs Of Platelet Activation,
2023
University of Kentucky
Ecohiv-Infected Mice Show No Signs Of Platelet Activation, Hammodah Rawhi Hammodah Alfar, Dominic Ngima Nthenge-Ngumbau, Kathryn E. Saatman, Sidney W. Whiteheart
Saha Cardiovascular Research Center Faculty Publications
Platelets express several surface receptors that could interact with different viruses. To understand the mechanisms of HIV-1′s interaction with platelets, we chose the EcoHIV model. While EcoHIV is an established model for neuroAIDS, its effects on platelets are ill-defined. Our results indicate that EcoHIV behaves differently from HIV-1 and is cleared from circulation after 48 h post- infection. The EcoHIV course of infection resembles an HIV-1 infection under the effects of combined antiretroviral therapy (cART) since infected mice stayed immunocompetent and the virus was readily detected in the spleen. EcoHIV-infected mice failed to become thrombocytopenic and showed no signs of …
Exploring The Potential Of Tesla Valve For Filtering And Sorting Microscale Active Swimmers: A Computational Study,
2023
University of Arkansas, Fayetteville
Exploring The Potential Of Tesla Valve For Filtering And Sorting Microscale Active Swimmers: A Computational Study, Ariel Rogers, Zhiyao He, Yong Wang
Physics Faculty Publications and Presentations
It is well-known that a Tesla valve allows fluids to flow unidirectionally without moving parts; however, how Tesla valves interact with active matters and the potential applications of Tesla valves in biology remain largely unexplored. Here, we present a computational study on the potential use of Tesla valves for filtering and sorting microscale active swimmers such as bacteria. We investigated the behavior of microscale swimmers passing through the Tesla valve at different linear and angular velocities using numerical simulations and quantified the diodicity of the Tesla valve for active swimmers. Our results demonstrate that the Tesla valve can effectively filter …
Characterizing The Regulation Of Candida Albicans Hyphal Morphogenesis In The Context Of Host Macrophages,
2023
The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
Characterizing The Regulation Of Candida Albicans Hyphal Morphogenesis In The Context Of Host Macrophages, Hannah Wilson
Dissertations and Theses (Open Access)
Candida albicans is a commensal fungus that resides on the skin, mucosal surfaces, and in the gut of an estimated 80% of individuals. Though generally harmless, C. albicans is capable of causing uncomfortable mucosal infections as well as deadly disseminated infections depending on the immune status of the host. Although antifungal therapeutics exist, the mortality rate associated with disseminated disease still lingers around 50%. Further, C. albicans is the fourth most common cause of all bloodstream infections and continues to present as a major clinical issue with less than satisfactory treatment options. For this reason, it is imperative to understand …
Enhancing Urban Water Quality Through Biological-Chemical Treatment: Aquatic Macroinvertebrate Community And Temporal Chlorophyll-A Response,
2023
University of Nebraska-Lincoln
Enhancing Urban Water Quality Through Biological-Chemical Treatment: Aquatic Macroinvertebrate Community And Temporal Chlorophyll-A Response, Matthew Chaffee
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
With a growing human population, urbanization is impeding a plethora of natural waterways. Of these, urban ponds play a vital role in nutrient sequestration, flood prevention, and habitat sanctuaries. However, nutrient loading can reduce habitat effectiveness and promote harmful algae blooms. To reduce internal nutrient loads, a biological-chemical treatment strategy consisting of floating treatment wetlands (FTWs) and lanthanum were applied to two urban retention ponds, Densmore and Wilderness Ridge Ponds. To measure effectiveness, chlorophyll-a samples were collected and correlated with Sentinel-2. A novel band algorithm termed 3BR1 produced a strong correlation (R2 = 0.72) to physical chlorophyll-a …
Intestinal Microbiome, Fecal Fermentation Profile, And Health Indices In Hiv Infected Men Versus Non-Infected Controls,
2023
East Tennessee State University
Intestinal Microbiome, Fecal Fermentation Profile, And Health Indices In Hiv Infected Men Versus Non-Infected Controls, Mary Andreae, Mary C. Andreae Mrs
Electronic Theses and Dissertations
Many HIV-positive (HIV+) males on Highly Active Anti-Retroviral Therapy (HAART) experience metabolic abnormalities, including Non-Alcoholic Fatty Liver Disease (NAFLD) and lipodystrophy. The intestinal microbiota and short chain fatty acids (SCFA), participate in bidirectional communication with their host. Dysbiosis in HIV+ males on HAART demonstrate a Prevotella-rich enterotype shaped by multiple factors including, medications, adiposity, diet, intestinal permeability, and lifestyle; our objective was to investigate these factors. 19 HIV+ and 21 HIV- males were enrolled. BMI and hip-to-waist ratio (H:W) were obtained, and FibroScan for liver health. Intestinal permeability markers Claudin-21, flagellin, and intestinal fatty acid binding protein (IFABP) in …
Immunization With The Amino-Terminus Region Of Dense Granule Protein 6 (Gra6) Of Toxoplasma Gondii Activates Cd8+ Cytotoxic T Cells Capable Of Removing Tissue Cysts Of The Parasite Through Antigen Presentation By Human Hla-A2.1,
2023
University of Kentucky
Immunization With The Amino-Terminus Region Of Dense Granule Protein 6 (Gra6) Of Toxoplasma Gondii Activates Cd8+ Cytotoxic T Cells Capable Of Removing Tissue Cysts Of The Parasite Through Antigen Presentation By Human Hla-A2.1, Rajesh Mani, Corinne Mercier, Marie-France Delauw, Yasuhiro Suzuki
Markey Cancer Center Faculty Publications
CD8þ T cells from HLA-A2.1-transgenic mice, but not wild-type mice, immunized with the amino- terminus region (aa 41e152) of dense granule protein 6 (GRA6Nt) of Toxoplasma gondii secreted large amounts of perforin and granzyme B in response to GRA6Nt through antigen presentation by HLA-A2.1 in vitro. When those CD8þ T cells were transferred into chronically infected HLA-A2.1-expressing NSG mice deficient in T cells, cerebral cyst burden of the recipients of HLA-A2.1-transgenic T cells, but not of WT T cells, became significantly less than that of control mice with no cell transfer. Furthermore, the significant reduction of the cyst burden by …
Development Of Novel Methods To Study Host-Microbe Interactions In The Larval Zebrafish Gastrointestinal Tract,
2023
The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
Development Of Novel Methods To Study Host-Microbe Interactions In The Larval Zebrafish Gastrointestinal Tract, Anh K. Trinh Nguyen
Dissertations and Theses (Open Access)
The dynamic nature and inaccessible location of the intestine pose significant challenges to the study of intestinal physiology and pathology. Zebrafish larvae, possessing optical transparency and genetic tractability, offer an accessible and clinically relevant model for investigating dynamic events in the intestine via time-lapse imaging. In the first part of this work, I discuss our efforts to optimize the parameters of a foodborne infection assay using paramecia as a vehicle. This method provides an effective, high-throughput alternative to infection via immersion or oral gavage, and replicates the most common route of transmission of gastrointestinal (GI) infection in humans. The foodborne …
Detection Of Dsrab Operon Expression In Desulfotalea Psychrophila Cells Subjected To Simulated Martian Conditions Of Temperature And Regolith's Sulphate Minerals Composition,
2023
University of Arkansas, Fayetteville
Detection Of Dsrab Operon Expression In Desulfotalea Psychrophila Cells Subjected To Simulated Martian Conditions Of Temperature And Regolith's Sulphate Minerals Composition, Sergio Mosquera, Mack Ivey, Vincent F. Chevrier
Biological Sciences Faculty Publications and Presentations
Discoveries of transient liquid water in the Martian polar caps and the presence of liquid lakes and subsurface oceans in icy satellites have increased the interest of scientists in the capabilities of terrestrial extremophiles to grow and remain metabolically active in these extreme environments. The principal goal of this research is to understand the metabolic capacity of the anaerobic psychrophile, Desulfotalea psychrophila, cultured at subfreezing temperatures in media containing various concentrations of sulphate minerals. In this regard, our experiments focused on the detection of D. psychrophila survival and active metabolism, employing a biochamber that can recreate Martian temperatures. Using …
The Developmental Process For Adenoviral Vectored Canine Influenza Vaccines,
2023
University of Nebraska-Lincoln
The Developmental Process For Adenoviral Vectored Canine Influenza Vaccines, Nicholas Jeanjaquet
School of Biological Sciences: Dissertations, Theses, and Student Research
Canine Influenza Virus (CIV) is a recently emerged branch of Influenza A virus that is highly infectious in dogs. The first strain was isolated in 2003, and it has quickly become endemic in areas with dense dog populations, most notably in Asia. The proximity of dogs to humans, along with their potential to serve as mixing vessels for reassortment, raises concern for possible zoonotic transmission and a potential human pandemic. Available vaccines are not frequently updated and struggle to prevent the spread of currently circulating strains. This highlights the need for a new and more effective vaccine. We outline the …
Breaking Virulent: The Coincidental Evolution Of Virulence Factors In Bacteria.,
2023
University of Louisville
Breaking Virulent: The Coincidental Evolution Of Virulence Factors In Bacteria., Rhiannon Emmanuelle Cecil
Electronic Theses and Dissertations
Understanding how innocuous organisms can evolve to be pathogenic to humans is of increasing global concern. Further, understanding how existing pathogens may evolved to be more virulent is also vital to our ability to provide healthcare to people afflicted with diseases that promote chronic bacterial infections, such as cystic fibrosis. With the rise of antibiotic resistance in both bacteria and fungi it is paramount that new therapeutics are identified. Understanding what mutations occur that result in increased virulence in microbes can potentially provide new targets for antimicrobial drugs to combat antibiotic resistance. The Coincidental Evolution Hypothesis is a fundamental hypothesis …
Identification And Characterization Of Geotrichum Candidum Isolates From Peach With Reduced Sensitivity To Propiconazole,
2023
Clemson University
Identification And Characterization Of Geotrichum Candidum Isolates From Peach With Reduced Sensitivity To Propiconazole, Sara Price
All Theses
Geotrichum candidum Link (1809) is a yeast-like fungus that causes sour rot of stone fruits, including peach (Prunus persica). The disease caused damage between 2021 and 2023 to the peach crop kept in cold storage at a commercial farm in South Carolina despite preharvest and postharvest applications of fungicides. Isolates from 41 symptomatic fruit, from a South Carolina (SC) commercial packing house, as well as other isolates obtained from South Carolina were identified to be G. candidum based on species-specific PCR amplifications of the β tubulin gene. Commonly used postharvest fungicide fludioxonil had no effect on G. candidum mycelial growth …
Lactic Acid Bacterial Surface
Display Of Scytovirin Inhibitors For
Anti-Ebolavirus Infection,
2023
University of Nebraska - Lincoln
Lactic Acid Bacterial Surface Display Of Scytovirin Inhibitors For Anti-Ebolavirus Infection, Joshua Wiggins, Ngan Nguyen, Wenzhong Wei, Leah Liu Wang, Haley Hollingsead Olson, Shi-Hua Xiang
Nebraska Center for Virology: Faculty Publications
Scytovirin (SVN) is a lectin from cyanobacteria which has a strong inhibitory activity against Ebola virus infection. We engineered scytovirin as the inhibitor for surface display of lactic acid bacteria to block Ebola virus infection. Two different bacterial strains (Lactobacillus casei and Lactococcus lactis) were successfully engineered for scytovirin expression on the bacterial surface. These bacteria were found to be effective at neutralizing pseudotyped Ebolavirus in a cell-based assay. This approach can be utilized for prophylactic prevention, as well as for treatment. Since lactic acid bacteria can colonize the human body, a long-term efficacy could be achieved. Furthermore, …
A Novel Subcluster Of Closely Related Bacillus Phages With Distinct Tail Fiber/Lysin Gene Combinations,
2023
Duke University
A Novel Subcluster Of Closely Related Bacillus Phages With Distinct Tail Fiber/Lysin Gene Combinations, Rachel E. Loney, Veronique A. Delesalle, Brianne E. Chaudhry, Megan Czerpak, Alexandra A. Guffey, Leo Goubet-Mccall, Michael Mccarty, Madison S. Strine, Natalie T. Tanke, Albert C. Vill, Gregory P. Krukonis
Biology Faculty Publications
Bacteriophages (phages) are the most numerous entities on Earth, but we have only scratched the surface of describing phage diversity. We isolated seven Bacillus subtilis phages from desert soil in the southwest United States and then sequenced and characterized their genomes. Comparative analyses revealed high nucleotide and amino acid similarity between these seven phages, which constitute a novel subcluster. Interestingly, the tail fiber and lysin genes of these phages seem to come from different origins and carry out slightly different functions. These genes were likely acquired by this subcluster of phages via horizontal gene transfer. In conjunction with host range …
Investigating The Role Of The Metabolite Je As An Inflammatory Modulator,
2023
Loyola University of Chicago Graduate School
Investigating The Role Of The Metabolite Je As An Inflammatory Modulator, Rim Ahmed Noufal
Master's Theses
Innate immunity represents the first line of defense against pathogens that invade our bodies. It greatly relies on a family of abundant receptors called pathogen recognition receptors (PRRs). These are germ-line encoded proteins abundant in immune cells like macrophages, dendritic cells, and natural killer cells. PRRs encompass five categories, one of which includes toll-like receptors (TLRs). TLRs could either be located on the surface of the cells or intracellularly. The mechanism by which TLRs act is through recognizing a myriad of foreign structures derived from microbes, such as proteins, lipids, and carbohydrates as well as their genetic material. Generally, these …
Investigations Of Emerging Zoonotic And Vector-Borne Viruses At The Juncture Of Environmental, Human, And Animal Health,
2023
Louisiana State University and Agricultural and Mechanical College
Investigations Of Emerging Zoonotic And Vector-Borne Viruses At The Juncture Of Environmental, Human, And Animal Health, Erik A. Turner
LSU Doctoral Dissertations
Reemerging and emerging zoonotic viruses are some of the most important public and One Health threats facing the world. Understanding the transmission dynamics of these viruses is vital to reducing the disease burden, and one of the key aspects to these transmission systems is the environmental vertex of the One Health triangle. SARS-CoV-2 has only recently emerged as a global public health threat, and within 4 years, over 700 million cases have been reported. As a novel pathogen, many aspects of transmission, including environmental fomite transmission and disinfection, were initially a glaring gap in knowledge. Additionally, while chikungunya virus (CHIKV) …
