Microbiology Commons^™

Exploring Pristine Microbial Ecosystems From The Uninhabited Volcanic Selvagens Islands, Pedro N. Jiménez-Morillo, Sara Gutiérrez-Patricio, Vera Palma, Ana Teresa Caldeira, Nicasio Tomás Jiménez-Morillo, Ana Z. Miller

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Recent Advances In The Microbiology Of Permanently Stratified, Hypersaline Lakes Of Transylvanian Basin (Central Romania), Horia Leonard Banciu, Ionuț Mădălin Gridan, Adrian Vasile Zety, Ioana Drăghici, Cristina Mircea, Michalis Daniel Lazăr, Andreea Melisa Tripon, Doriana Mădălina Buda, Adorján Cristea

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Given To Salt: Novel Insights Into The Diversity Of Halophilic Microorganisms And Their Functioning In Hypersaline Ecosystems, Aharon Oren

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Microbial Colonization Of New Lava Tubes Formed By The Fagradalsfjall Volcano’S Eruption (Iceland), Ettore Lopo, Francesco Sauro, Ana Zelia Miller, Bogdan Petroniu Onac, Martina Cappelletti

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Onsite Microbial Analyses In One Of The Most Remote Quartzite Subterranean Environment On Earth: Imawarì Yeuta On Venezuelan Tepuis, Martina Cappelletti

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

First Biogeochemical Study Of Mira De Aire Cave: The Biggest Karstic System In Portugal, Alba Gómez-Arias, Julio Castillo, Nicasio Tomás Jiménez-Morillo, Beatriz Cubero, Sara Gutiérrez-Patricio, Pedro N. Jimenez-Morillo, José A. Gonzalez-Perez, José M. De La Rosa, Ana Z. Miller

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Sampling And Dna Analysis Of Extreme Environments, Scott Tighe

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

The Ecosystem Of A Remarkable Glaciovolcanic Cave At Mount Meager, British Columbia, Canada, Christian Stenner, Kathleen Graham, Morgan Cable, Glyn Williams-Jones, Jared J. Clance, Jacob Shaffer, Jill Mikucki, Anna Szynkiewicz, Olivia Vinnes, Michael Paton, Jeremy Nash

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Cave Ice Microbiome – Reservoir For New Biomolecules In Medicine And Biotechnologies, Victoria Ioana Paun, Corina Itcus, Carmen Mariana Chifiriuc, Paris Lavin, Cristina Purcarea

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Antarctic Glacier Melting Modeling Distribution Of Bacterial Community Structure, Corina Itcus, Constantin Marin, Soon Gyu Hong, Manuela Elisabeta Sidoroff, Cristian Coman, Cristina Purcarea

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Molecular Detection Of Mycoplasma Spp. And Its Associated Risk Factors In Domestic Cats In The City Of Ibagué, Tolima, Karen D. Osorio Muñoz Dr., Iang S. Rondon Barragan Prof.

The Thai Journal of Veterinary Medicine

Feline hemoplasmosis is a disease with worldwide distribution, mainly in tropical countries, where its transmission is favored by the presence of vectors such as fleas and ticks. In Colombia, the diagnosis of the disease in small animals has been limited to the identification of the pathogen in blood smears, which have low sensitivity and specificity, demanding methods of higher diagnostic value, such as those based on molecular techniques. The objective of the present study was to investigate the prevalence of Mycoplasma spp. in felines from the city of Ibagué using the PCR and the associated risk factors. Briefly, blood samples …

Genomic Analysis Of Enterobacter Species Isolated From Patients In United States Hospitals, Fred C. Tenover, Isabella A. Tickler

Guidelines, Recommendations, and Other Resources on Antimicrobial Resistance

Abstract: We analyzed the whole genome sequences (WGS) and antibiograms of 35 Enterobacter isolates, including E. hormaechei and E. asburiae, and the recently described E. bugandensis, E. kobei, E. ludwigii, and E. roggenkampii species. Isolates were obtained from human blood and urinary tract infections in patients in the United States. Our goal was to understand the genetic diversity of antimicrobial resistance genes and virulence factors among the various species. Thirty-four of 35 isolates contained an AmpC class blaACT allele; however, the E. roggenkampii isolate contained blaMIR-5. Of the six Enterobacter isolates resistant to ertapenem, imipenem, and meropenem, …

The Roles Of Cd4+ T Cell Help, Sex, And Dose In The Induction Of Protective Cd8+ T Cells Against A Lethal Poxvirus By Mrna-Lnp Vaccines, Samita Kafle, Brian Montoya, Lingjuan Tang, Ying Tam, Hiromi Muramatsu, Norbert Pardi, Luis Sigal

Department of Microbiology and Immunology Faculty Papers

The role of CD4⁺ T cells in the induction of protective CD8⁺ T cells by mRNA lipid nanoparticle (LNP) vaccines is unknown. We used B6 or Tlr9^−/− mice depleted or not of CD4⁺ T cells and LNP vaccines loaded with mRNAs encoding the ectromelia virus (ECTV) MHC class I H-2 K^b-restricted immunodominant CD8⁺ T cell epitope TSYKFESV (TSYKFESV mRNA-LNPs) or the ECTV EVM158 protein, which contains TSYKFESV (EVM-158 mRNA-LNPs). Following prime and boost with 10 μg of either vaccine, K^b-TSYKFESV-specific CD8⁺ T cells fully protected male and female mice from …

Final Outcomes From A Phase 2 Trial Of Posoleucel In Allogeneic Hematopoietic Cell Transplant Recipients, Sanjeet S Dadwal, Rajat Bansal, Michael W Schuster, Jean A Yared, Gary Douglas Myers, Michelle Matzko, Sama Adnan, David Mcneel, Julie Ma, Sarah A Gilmore, Spyridoula Vasileiou, Ann M Leen, Joshua A Hill, Jo-Anne H Young

Faculty, Staff and Students Publications

Allogeneic hematopoietic cell transplantation (allo-HCT) recipients are susceptible to viral infections. We conducted a phase 2 trial evaluating the safety and rate of clinically significant infections (CSIs; viremia requiring treatment or end-organ disease) after infusion of posoleucel, a partially HLA-matched, allogeneic, off-the-shelf, multivirus-specific T-cell investigational product for preventing CSIs with adenovirus, BK virus, cytomegalovirus, Epstein-Barr virus, human herpesvirus-6, or JC virus. This open-label trial enrolled allo-HCT recipients at high risk based on receiving grafts from umbilical cord blood, haploidentical, mismatched, or matched unrelated donors; post-HCT lymphocytes of < 180/mm3; or use of T-cell depletion. Posoleucel dosing was initiated within 15 to 49 days of allo-HCT and subsequently every 14 days for up to 7 doses. The primary end point was the number of CSIs due to the 6 target viruses by week 14. Of the 26 patients enrolled, only 3 (12%) had a CSI by week 14, each with a single target virus. In vivo expansion of functional virus-specific T cells detected via interferon-γ enzyme-linked immunosorbent spot assay was associated with viral control. Persistence of posoleucel-derived T-cell clones for up to 14 weeks after the last infusion was confirmed by T-cell–receptor deep sequencing. Five patients (19%) had acute graft-versus-host disease grade 2 to 4. No patient experienced cytokine release syndrome. All 6 deaths were due to relapse or disease progression. allo-HCT recipients at high risk who received posoleucel had low rates of CSIs from 6 targeted viruses. Repeat posoleucel dosing was generally safe and well tolerated and associated with functional immune reconstitution.

Disruption Of Cochlear Development By Murine Cytomegalovirus Infection And Therapeutic Approaches That Prevent Hearing Loss, Matthew Smith

All ETDs from UAB

Human cytomegalovirus (HCMV) is a ubiquitous virus, infecting greater than 50% of most populations worldwide. Although rarely causing symptomatic infection in immunocompetent individuals, HCMV can cause life-threatening and severe end-organ disease in immunocompromised hosts including the developing fetus. HCMV can cross the placenta and is the most common viral infection of the developing fetus. Most newborn infants with congenital HCMV infection (cCMV) exhibit no clinical evidence of infection; however, up to 15% will develop long term neurodevelopmental sequelae including sensorineural hearing loss (SNHL). No prophylactic vaccines or effective antiviral treatments exist for cCMV infection, which may in part be secondary …

Pseudomonas Aeruginosa And Tobramycin Effects On Aspergillus Fumigatus Infection In Cystic Fibrosis Airway, Tuan Anh Nguyen

All ETDs from UAB

Aspergillus fumigatus (Af) is one of the most common molds in the airways of people with Cystic Fibrosis (pwCF). The infection of Af is often preceded by Pseudomonas aeruginosa (PsA) infection and its correlated treatment. PsA is considered a highly prevalent bacteria in the CF lung. PwCF suffers from complex and sophisticated coinfections within the airway that negatively impact the health outcomes of patients. Despite this, why fungal and bacterial coinfection occurs in pwCF is still being determined. However, abnormal mucus production and its characteristics are suspected of creating an environment that promotes Af germination and disease progression. This study …

Investigating The Impacts Of Staphylococcus Aureus Small Colony Variant Infection In The Cystic Fibrosis Rat Lung, Gretchen E. Bollar

All ETDs from UAB

Cystic fibrosis (CF) is an autosomal-recessive genetic disease characterized by recurrent and chronic airway infections. Staphylococcus aureus is currently the most common organism isolated from the CF lung, but its role as a pathogen has been historically difficult to characterize due to a broad range of genetic diversity and subtypes seen across infectious strains. Small colony variants (SCVs) are a subpopulation of S. aureus marked by slow growth rate and impaired metabolism that have been associated with worse respiratory outcomes in people with CF (pwCF). SCVs also exhibit altered colony morphology and can confound the results of traditional diagnostic assays, …

Fcrider: A Recombinant Fc Nanoparticle With Endogenous Adjuvant Activities For Hybrid Immunization, Changchuin Mao, Karen Eberle, Xiaojie Chen, Yiming Zhou, Jun Li, Hong Xin, Wenda Gao

School of Graduate Studies Faculty Publications

Active immunization (vaccination) induces long-lasting immunity with memory, which takes weeks to months to develop. Passive immunization (transfer of neutralizing antibodies) provides immediate protection, yet with high cost and effects being comparatively short-lived. No currently approved adjuvants are compatible with formulations to combine active and passive immunizations, not to mention their huge disparities in administration routes and dosage. To solve this, we engineered the Fc fragment of human IgG1 into a hexamer nanoparticle and expressed its afucosylated form in Fut8−/− CHO cells, naming it “FcRider.” FcRider is highly soluble with long-term stability, easily produced at high levels equivalent to those …

Cognition And Inflammation In Youth With Perinatal Hiv, Megan Niehaus, Julie Mannarino, Jacob Bolzenius, Tori Frerichs, Robert Paul

Undergraduate Research Symposium

Background: Perinatally acquired HIV (PHIV) remains a major global health challenge. Approximately 90% of the global population of PHIV reside in low- or middle-income countries (LMICs) where access to antiretroviral therapy (ART) remains incomplete, particularly for PHIV. With PHIV, the brain is exposed to the virus during critical periods of brain development without the immunological benefits from ART. Unlike adults with horizontally acquired HIV whose nadir CD4+ Tcell count is a strong correlate of cognitive difficulties that persist after sustained use of ART, cognitive symptoms among PHIV youth are independent of traditional HIV disease metrics. Prior studies indicate that PHIV …

Bean Yellow Mosaic Virus And Its Management In Lupins, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Bean yellow mosaic virus (BYMV) is an aphid-transmitted virus that commonly causes necrotic symptoms that kills lupin plants when infected early before pod set. When plants are infected after pod set, black pods develop (known as black pod syndrome).

BYMV is found predominantly in high rainfall areas of the Western Australian (WA) grainbelt. This page outlines the symptoms, diagnosis, factors favouring disease risk and spread, yield and quality impacts, and management for BYMV in lupin crops in WA