Diatom Voucher Flora And Comparison Of Collection Methods For Biodiversity Hotspot Upper Three Runs Creek,
2020
Georgia College & State University
Diatom Voucher Flora And Comparison Of Collection Methods For Biodiversity Hotspot Upper Three Runs Creek, Katie M. Johnson
Biology Theses
Surface freshwater is a scarce resource. Due to the scarcity and necessity of this resource, it is imperative that its quality is routinely monitored. One way of monitoring water quality is through biological assessments, which include examining algal assemblages. Regionally, little remains known of algal taxa in the southeastern United States. In the past, the Academy of Natural Sciences of Philadelphia (ANSP) conducted biological assessments along the Savannah River finding Gomphonema parvulum (Kützing) Kützing 1849 to be a dominant taxon rendering assessments inconclusive. Recent studies have provided evidence that species complexes and semi-cryptic taxa have been identified within the Gomphonema …
Quantifying Nutrient Throughput And Dom Production By Algae In Continuous Culture,
2020
Case Western Reserve University
Quantifying Nutrient Throughput And Dom Production By Algae In Continuous Culture, Anne Willem Omta
Faculty Scholarship
Freshwater and marine algae can balance nutrient demand and availability by regulating uptake, accumulation and exudation. To obtain insight into these processes under nitrogen (N) and phosphorus (P) limitation, we reanalyze published data from continuous cultures of the chlorophyte Selenastrum minutum . Based on mass budgets, we argue that much of the non-limiting N and P had passed through the organisms and was present as dissolved organic phosphorus or nitrogen (DOP or DON). We construct a model that describes the production of biomass and dissolved organic matter (DOM) as a function of the growth rate. A fit of this model …
Development Of Pcr-Based Detection System For Soft Rot Pectobacteriaceae Pathogens Using Molecular Signatures,
2020
Tennessee State University
Development Of Pcr-Based Detection System For Soft Rot Pectobacteriaceae Pathogens Using Molecular Signatures, Md Niamul Kabir, Ali Taheri, C. Korsi Dumenyo
Agricultural and Environmental Sciences Faculty Research
Pectobacterium and Dickeya species, usually referred to as soft rot Enterobacteriaceae, are phytopathogenic genera of bacteria that cause soft rot and blackleg diseases and are responsible for significant yield losses in many crops across the globe. Diagnosis of soft rot disease is difficult through visual disease symptoms. Pathogen detection and identification methods based on cultural and morphological identification are time-consuming and not always reliable. A polymerase chain reaction (PCR)-based detection method with the species-specific primers is fast and reliable for detecting soft rot pathogens. We have developed a specific and sensitive detection system for some species of soft rot Pectobacteriaceae …
Life Line March 2020,
2020
Otterbein University
Life Line March 2020, Otterbein Biology And Earth Science Department
Life Line - The Biology Department Newsletter
The Department Welcomes Dr. Sinn; Modeling to Help with Global Research; The Paradise of Bombs Revisited; The End of an Era in Conesville Ohio; Reports from two recipients of the Melinda Phinney Award for Pre-Med Travel Experiences; Preparing Skins for Study; Genomics in the Jungle; Zoo Program Gets New T -Shirt and Sweatshirt Designs; Aquarium Track Dives Right In; Dr. Svitana’s Sabbatical Accomplishments;Biology Behind Bars; Grossology: The Science of Icky, Sticky, Disgusting Things.
A Positive Feedback Loop Of Hippo- And C-Jun-Amino-Terminal Kinase Signaling Pathways Regulates Amyloid-Beta-Mediated Neurodegeneration,
2020
University of Dayton
A Positive Feedback Loop Of Hippo- And C-Jun-Amino-Terminal Kinase Signaling Pathways Regulates Amyloid-Beta-Mediated Neurodegeneration, Madison Irwin, Meghana Tare, Aditi Singh, Oorvashi Roy Puli, Neha Gogia, Matthew Riccetti, Prajakta Deshpande, Madhuri Kango-Singh, Amit Singh
Biology Faculty Publications
Alzheimer's disease (AD, OMIM: 104300) is an age-related disorder that affects millions of people. One of the underlying causes of AD is generation of hydrophobic amyloid-beta 42 (Aβ42) peptides that accumulate to form amyloid plaques. These plaques induce oxidative stress and aberrant signaling, which result in the death of neurons and other pathologies linked to neurodegeneration. We have developed a Drosophila eye model of AD by targeted misexpression of human Aβ42 in the differentiating retinal neurons, where an accumulation of Aβ42 triggers a characteristic neurodegenerative phenotype. In a forward deficiency screen to look for genetic modifiers, we identified a molecularly …
The Gut Microbiota Of A Wild American Black Bear (Ursus Americanus) Population,
2020
Northern Michigan University
The Gut Microbiota Of A Wild American Black Bear (Ursus Americanus) Population, Sierra Gillman
All NMU Master's Theses
The gut microbiome (GMB), the mutualistic microbial communities located in the gastrointestinal tract (GIT), has co-evolved in vertebrates to perform micro-ecosystem services to facilitate physiological functions. Despite the key role of the GMB in host health, wildlife managers have been slow to consider the ramifications of anthropogenic pressures to wildlife-GMB diversity. For example, although diet is one of the most influential determinants of a host’s GMB, many wildlife agencies allow baiting with human-provisioned foods to facilitate the harvest of wildlife such as American black bear (Ursus americanus). Additionally, much of our knowledge of wildlife-GMB relationships is based on …
Non-Tuberculous Mycobacterial Infections-A Neglected And Emerging Problem,
2020
Aga Khan University
Non-Tuberculous Mycobacterial Infections-A Neglected And Emerging Problem, Imran Ahmed, Simon Tiberi, Joveria Farooqi, Kauser Jabeen, Dorothy Yeboah-Manu, Giovanni Battista Migliori, Rumina Hasan
Department of Pathology and Laboratory Medicine
Non-tuberculous mycobacteria (NTM) are ubiquitous dwellers of environmental niches and are an established cause of natural and nosocomial infections. The incidence of NTM infections is rising owing to a growing population of immunocompromised and vulnerable individuals, complex medical and surgical procedures, as well as increased awareness and diagnostic capabilities. The prevalence of different NTM varies between continents, regions, and countries. The true global burden of pulmonary and extrapulmonary disease is unknown and estimates are subject to under and/or over-estimation. Diagnosis requires confirmation by isolation of NTM along with clinical and radiological criteria, which may be suboptimal at all levels. Susceptibility …
Gene Expression Analysis Of Effectors Involved In Early Interaction Of Banana And Fusarium Oxysporum F. Sp. Cubense,
2020
Universiti Malaya
Gene Expression Analysis Of Effectors Involved In Early Interaction Of Banana And Fusarium Oxysporum F. Sp. Cubense, Nee Kiew Poon
Student Works (2020-2029)
Banana is a popular but disease-laden fruit crop. It is very susceptible to various phytopathogens such as viruses, bacteria, fungi and nematodes. A particularly infectious and economically important fungal disease in banana is Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc). The disease has been plaguing the banana industry since late 19th century. Despite the early discovery of this phytopathogen of banana, little is known on its the mechanism of pathogenicity. In other plants, fungal effectors have been found to function in manipulating plant mechanisms during infection and many have been identified and characterized. In this study, the …
Transposon Mutagenesis In Mycobacterium Kansasii Links A Small Rna Gene To Colony Morphology And Biofilm Formation And Identifies 9,885 Intragenic Insertions That Do Not Compromise Colony Outgrowth,
2020
CUNY Graduate Center
Transposon Mutagenesis In Mycobacterium Kansasii Links A Small Rna Gene To Colony Morphology And Biofilm Formation And Identifies 9,885 Intragenic Insertions That Do Not Compromise Colony Outgrowth, William C. Budell, Gabrielle A. Germain, Niklas Janisch, Zaid Mckie-Krisberg, Anitha D. Jayaprakash, Andrew E. Resnick, Luis E. N. Quadri
Publications and Research
Mycobacterium kansasii (Mk) is a resilient opportunistic human pathogen that causes tuberculosis-like chronic pulmonary disease and mortality stemming from comorbidities and treatment failure. The standard treatment of Mk infections requires costly, long-term, multidrug courses with adverse side effects. The emergence of drug-resistant isolates further complicates the already challenging drug therapy regimens and threatens to compromise the future control of Mk infections. Despite the increasingly recognized global burden of Mk infections, the biology of this opportunistic pathogen remains essentially unexplored. In particular, studies reporting gene function or generation of defined mutants are scarce. Moreover, no transposon (Tn) mutagenesis tool has been …
Invasive Vegetation Affects Amphibian Skin Microbiota And Body Condition,
2020
Department of Environmental Science, Policy, and Management, University of California, Berkeley
Invasive Vegetation Affects Amphibian Skin Microbiota And Body Condition, Obed Hernandez-Gomez, Allison Q. Byrne, Alex R. Gunderson, Thomas S. Jenkinson, Clay F. Noss, Andrew P. Rothstein, Molly C. Womack, Erica B. Rosenblum
Natural Sciences and Mathematics | Faculty Scholarship
Invasive plants are major drivers of habitat modification and the scale of their impact is increasing globally as anthropogenic activities facilitate their spread. In California, an invasive plant genus of great concern is Eucalyptus. Eucalyptus leaves can alter soil chemistry and negatively affect underground macro- and microbial communities. Amphibians serve as excellent models to evaluate the effect of Eucalyptus invasion on ground-dwelling species as they predate on soil arthropods and incorporate soil microbes into their microbiotas. The skin microbiota is particularly important to amphibian health, suggesting that invasive plant species could ultimately affect amphibian populations. To investigate the potential for …
Emerging Priorities For Microbiome Research,
2020
Drexel University
Emerging Priorities For Microbiome Research, Chad M. Cullen, Kawalpreet K. Aneja, Sinem Beyhan, Clara E. Cho, Stephen Woloszynek, Matteo Convertino, Sophie J. Mccoy, Yanyan Zhang, Matthew Z. Anderson, David Alvarez-Ponce, Ekaterina Smirnova, Lisa Karstens, Pieter C. Dorrestein, Hongzhe Li, Ananya Sen Gupta, Et Al.
Nutrition, Dietetics, and Food Sciences Faculty Publications
Microbiome research has increased dramatically in recent years, driven by advances in technology and significant reductions in the cost of analysis. Such research has unlocked a wealth of data, which has yielded tremendous insight into the nature of the microbial communities, including their interactions and effects, both within a host and in an external environment as part of an ecological community. Understanding the role of microbiota, including their dynamic interactions with their hosts and other microbes, can enable the engineering of new diagnostic techniques and interventional strategies that can be used in a diverse spectrum of fields, spanning from ecology …
Ancyronyx Clisteri, A New Spider Riffle Beetle Species From Borneo, Redescription Of A. Sarawacensis Jäch Including A Description Of The Larva And New Distribution Data For A. Procerus Jäch Using Dna Barcodes (Coleoptera, Elmidae),
2020
Ateneo de Manila University
Ancyronyx Clisteri, A New Spider Riffle Beetle Species From Borneo, Redescription Of A. Sarawacensis Jäch Including A Description Of The Larva And New Distribution Data For A. Procerus Jäch Using Dna Barcodes (Coleoptera, Elmidae), Jan Kodada, Manfred A. Jach, Hendrik Freitag, Zuzana Čiamporová-Zaťovičová, Katarína Goffová, Dávid Selnekovič, Fedor Čiampor Jr
Biology Faculty Publications
Ancyronyx clisteri sp. nov. (Coleoptera, Elmidae) a new spider riffle beetle discovered from northern Borneo (Brunei; Sabah and Sarawak, Malaysia) and the larva of Ancyronyx sarawacensis Jäch are described. Illustrations of the habitus and diagnostic characters of the new species and the similar and highly variable A. sarawacensis are presented. Differences to closely related species, based on DNA barcodes and morphological characters, are discussed. Association of the larva and the imago of A. sarawacensis, and the occurrence of Ancyronyx procerus Jäch in Peninsular Malaysia and Sabah are confirmed by using COI mtDNA sequences.
Beneficial Endophytic Bacterial Populations Associated With Medicinal Plant Thymus Vulgaris Alleviate Salt Stress And Confer Resistance To Fusarium Oxysporum,
2020
Xinjiang Institute of Ecology and Geography
Beneficial Endophytic Bacterial Populations Associated With Medicinal Plant Thymus Vulgaris Alleviate Salt Stress And Confer Resistance To Fusarium Oxysporum, Osama Aballa Abdelshafy Mohamad, Jin-Bao Ma, Yong-Hong Liu, Daoyuan Zhang, Shao Hua, Shirkant Bhute, Brian P. Hedlund, Wen-Jun Li, Li Li
Life Sciences Faculty Research
As a result of climate change, salinity has become a major abiotic stress that reduces plant growth and crop productivity worldwide. A variety of endophytic bacteria alleviate salt stress; however, their ecology and biotechnological potential has not been fully realized. To address this gap, a collection of 117 endophytic bacteria were isolated from wild populations of the herb Thymus vulgaris in Sheikh Zuweid and Rafah of North Sinai Province, Egypt, and identified based on their 16S rRNA gene sequences. The endophytes were highly diverse, including 17 genera and 30 species. The number of bacterial species obtained from root tissues was …
A High-Fat/High-Protein, Atkins-Type Diet Exacerbates Clostridioides (Clostridium) Difficile Infection In Mice, Whereas A High-Carbohydrate Diet Protects,
2020
University of Nevada, Las Vegas
A High-Fat/High-Protein, Atkins-Type Diet Exacerbates Clostridioides (Clostridium) Difficile Infection In Mice, Whereas A High-Carbohydrate Diet Protects, Chrisabelle C. Mefferd, Shrikant S. Bhute, Jacqueline R. Phan, Jacob V. Villarama, Dung M. Do, Stephanie Alarcia, Ernesto Abel-Santos, Brian P. Hedlund
Chemistry and Biochemistry Faculty Research
Clostridioides difficile (formerly Clostridium difficile) infection (CDI) can result from the disruption of the resident gut microbiota. Western diets and popular weight-loss diets drive large changes in the gut microbiome; however, the literature is conflicted with regard to the effect of diet on CDI. Using the hypervirulent strain C. difficile R20291 (RT027) in a mouse model of antibiotic-induced CDI, we assessed disease outcome and microbial community dynamics in mice fed two high-fat diets in comparison with a high-carbohydrate diet and a standard rodent diet. The two high-fat diets exacerbated CDI, with a high-fat/high-protein, Atkins-like diet leading to severe CDI and …
Characterization Of A Species E Adenovirus Vector As A Zika Virus Vaccine,
2020
University of Nebraska - Lincoln
Characterization Of A Species E Adenovirus Vector As A Zika Virus Vaccine, Brianna L. Bullard, Brigette N. Corder, David N. Gordon, Theodore C. Pierson, Eric A. Weaver
Nebraska Center for Virology: Faculty Publications
The development of a safe and efficacious Zika virus (ZIKV) vaccine remains a global health priority. In our previous work, we developed an Adenovirus vectored ZIKV vaccine using a low-seroprevalent human Adenovirus type 4 (Ad4-prM-E) and compared it to an Ad5 vector (Ad5-prM-E). We found that vaccination with Ad4-prM-E leads to the development of a strong anti-ZIKV T-cell response without eliciting significant anti-ZIKV antibodies, while vaccination with Ad5-prM-E leads to the development of both anti-ZIKV antibody and T-cell responses in C57BL/6 mice. However, both vectors conferred protection against ZIKV infection in a lethal challenge model. Here we continued to characterize …
Surveillance Of Root-Associated Microbiome Of Oxalogenic Colocasia Esculenta (Linn) Plant Reveals Distinct Bacterial Species Diversity,
2020
National Centre for Cell Science
Surveillance Of Root-Associated Microbiome Of Oxalogenic Colocasia Esculenta (Linn) Plant Reveals Distinct Bacterial Species Diversity, Mangesh Vasant Suryavanshi, Nidhi Bharti, Shrikant Subhash Bhute, Vipin Chembili, Pooja Chavan, Yogesh Shripad Shouche
Life Sciences Faculty Research
Oxalic acids are widely distributed in tissues of various plants that can exacerbate the effect on other plant-grazing animals including humans. Bacterial communities had been demonstrated to specify with the rhizosphere of host type and which can differ with oxalogenic plant. The present study has been conducted with the primary objective of understanding the root-associated microbial communities in Colocasia esculenta, an oxalogenic plant and to recognize possible bacterial species that present the potential of having their capability to metabolize oxalates. Of the 852 sequences obtained, 311 corresponded to rhizosphere (S), 250 to rhizoplane (P) and 291 were from non-rhizospheric (NS) …
Through The Back Door: Proteins Escape Cells Without Conventional Permission,
2020
CUNY Graduate Center
Through The Back Door: Proteins Escape Cells Without Conventional Permission, Michael J. Cohen
Dissertations, Theses, and Capstone Projects
Proteins secreted to the extracellular environment play a fundamental role as signals, in metabolism, and a variety of other processes. The process of secretion through the endoplasmic reticulum and Golgi to the plasma membrane is well documented, and all cargo in this pathway contains a signal peptide. However, a variety of proteins secreted from eukaryotes lack a signal peptide and are called unconventionally secreted proteins. Here we discuss known mechanisms of unconventional protein secretion, as well as model proteins which follow characterized pathways. Additionally, we summarize the roles various unconventionally secreted proteins play outside of cells and suggest criteria for …
In Vitro Regeneration Of Dioscorea Alata With Anthocyanin And Antimicrobial Activity,
2020
Universiti Malaya
In Vitro Regeneration Of Dioscorea Alata With Anthocyanin And Antimicrobial Activity, Abdullah Sakinah
Student Works (2020-2029)
An efficient protocol was developed for rapid propagation and regeneration of the Dioscorea alata L. locally known as ‘ubi badak’ because of their rhinoceros-shaped tubers. In the present study, direct and indirect regeneration were induced to produce plantlets of Dioscorea alata L. For direct regeneration explants (leave, stem and node) were cultured on MS media supplemented with various combinations and concentrations of BAP and NAA to induce microshoots and roots formation. The optimum medium for microshoots formation was MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l NAA with the number of microshoot is 23.07±0.44 from node explant. While …
Hydrolysis Of Palm Oil Mill Effluent Using Enzymes From Spent Mushroom Substrate Of Pleurotus Pulmonarius (Fr.) Quel,
2020
Universiti Malaya
Hydrolysis Of Palm Oil Mill Effluent Using Enzymes From Spent Mushroom Substrate Of Pleurotus Pulmonarius (Fr.) Quel, Mat Yunan Nurul Anisa
Student Works (2020-2029)
Mushroom cultivation and palm oil industry in Malaysia have contributed to the large volumes of lignocellulosic residues. Lignocellulolytic enzymes profiles (lignin peroxidase, laccase, xylanase, endoglucanase, and β-glucosidase) of Pleurotus pulmonarius (parents strains UMP001 and UMP 002, and the hybrid UMH004) during growth in sawdust substrate bags were investigated. The enzymes extracted from the spent mushroom substrate (SMS) of the strain with the highest enzymes productivity was used to hydrolyse palm oil mill effluent (POME). Optimization of parameters for POME hydrolysis to release reducing sugar was conducted using central composite design (CCD) of response surface methodology (RSM). The potential of hydrolysed …
Abundance And Distribution Of Microplastics And Persistent Organic Pollutants In Selected Rivers Of Peninsular Malaysia,
2020
Universiti Malaya
Abundance And Distribution Of Microplastics And Persistent Organic Pollutants In Selected Rivers Of Peninsular Malaysia, Anuar Norkhairiyah
Student Works (2020-2029)
Nearly 70 to 80 percent of microplastics are believed to be transported to the sea through rivers. Yet, less attention has been given to the accumulation of microplastics in the freshwater environment. They are emerging anthropogenic contaminants on account of their potential to adsorb and/or release POPs from and/or to the surrounding environment, and become the vector transferring these pollutants from water to the food web. This study is aimed to explore the abundance and distribution of microplastics and POPs in six rivers of Peninsular Malaysia, which comprised of three rivers from the West Coast (Sepetang River, Serkam River, and …
